Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,324,469 (G)6→7 coding (341/1185 nt) atoB → acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,324,4631.G98.9% 556.4 / ‑1.4 174coding (335/1185 nt)atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (49/123);  total (49/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 228 total aligned reads displayed.

ATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324371‑2324550
                                                                                             |                                                                                        
aTTCACGGTCAATAAAGTATCTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGc                                                                                           <  1:2983289/93‑1 (MQ=255)
 ttCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑g                                                                                         >  1:4713870/1‑93 (MQ=255)
 ttCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑g                                                                                         <  1:5947650/93‑1 (MQ=255)
 ttCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑g                                                                                         >  2:3456047/1‑93 (MQ=255)
  tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑gg                                                                                        <  2:6521073/93‑1 (MQ=255)
   cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg                                                                                       <  1:1708106/93‑1 (MQ=255)
   cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg                                                                                       <  2:5086280/93‑1 (MQ=255)
    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑g                                                                                     >  1:3656157/1‑93 (MQ=255)
    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑g                                                                                     <  2:1821454/93‑1 (MQ=255)
     cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑gg                                                                                    >  2:5617956/1‑93 (MQ=255)
      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑ggg                                                                                   <  1:3482528/93‑1 (MQ=255)
       gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑gggg                                                                                  >  2:4818004/1‑92 (MQ=255)
        tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑ggggg                                                                                 >  1:3303008/1‑91 (MQ=255)
        tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTCCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑ggggt                                                                                 <  1:1250162/93‑3 (MQ=255)
         cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑ggggta                                                                                <  2:2140062/93‑4 (MQ=255)
         cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑ggggga                                                                                >  1:3969517/1‑90 (MQ=255)
          aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg‑ggggtat                                                                               <  1:163154/93‑5 (MQ=255)
            tAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATgg                                                                              <  2:1123942/93‑1 (MQ=255)
                gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGaaaa                                                                          <  2:5394611/93‑1 (MQ=255)
                gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCAAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGaaaa                                                                          <  1:4246486/93‑1 (MQ=255)
                  aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGGAAata                                                                        >  2:2537569/1‑93 (MQ=255)
                   tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAatat                                                                       <  1:518463/93‑1 (MQ=255)
                     tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGTGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGa                                                                     <  1:4781518/93‑1 (MQ=255)
                     tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGa                                                                     <  1:6332348/93‑1 (MQ=255)
                      ggTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGGAAAAATGAg                                                                    >  1:1484376/1‑93 (MQ=255)
                       gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGt                                                                   <  2:5808780/93‑1 (MQ=255)
                        ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGtt                                                                  <  2:6678657/93‑1 (MQ=255)
                          cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTa                                                                <  2:2433479/93‑1 (MQ=255)
                          cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTa                                                                <  1:5670160/93‑1 (MQ=255)
                          cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTa                                                                <  2:3299714/93‑1 (MQ=255)
                          cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTa                                                                <  1:515220/93‑1 (MQ=255)
                          cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTa                                                                >  2:531292/1‑93 (MQ=255)
                          cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCACGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGGAAATATGAGGTTa                                                                >  1:302099/1‑93 (MQ=255)
                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAg                                                               <  1:1536428/93‑1 (MQ=255)
                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAg                                                               >  1:1033081/1‑93 (MQ=255)
                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAg                                                               >  1:1148934/1‑93 (MQ=255)
                            ggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGc                                                              <  2:3397348/93‑1 (MQ=255)
                            ggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGc                                                              <  2:3681509/93‑1 (MQ=255)
                            ggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGGTGGAAAAAATGAGTTTTGc                                                              >  2:2563189/1‑93 (MQ=255)
                             gTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGcc                                                             <  1:3077300/93‑1 (MQ=255)
                             gTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGcc                                                             <  2:6022647/93‑1 (MQ=255)
                             gTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAAAATGAGGTTAggc                                                             >  2:393933/1‑91 (MQ=255)
                              tCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGGAAATATGAGTTTAGccc                                                            >  1:5809529/1‑93 (MQ=255)
                               cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGcccc                                                           <  2:1445620/93‑1 (MQ=255)
                               cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGcccc                                                           <  2:3126247/93‑1 (MQ=255)
                               cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGcccc                                                           >  2:5006732/1‑93 (MQ=255)
                                ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGccccc                                                          <  1:2034196/93‑1 (MQ=255)
                                  aaaaGTGTGGCGCTTGCCGCCGAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGGTGGGTATGGAAAATATGAGTTTAGCCCCCta                                                        <  1:2799833/93‑1 (MQ=255)
                                   aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGGTTAGCCCCCtac                                                       >  1:1010785/1‑93 (MQ=255)
                                   aaaGTGTGGCGCTTGCCGCCCAGGCCATGCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCcac                                                       <  1:3527621/93‑1 (MQ=255)
                                    aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCtact                                                      <  2:1965826/93‑1 (MQ=255)
                                           gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGa                                               >  2:4176084/1‑93 (MQ=255)
                                            cgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGAt                                              <  1:5411577/93‑1 (MQ=255)
                                             gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATg                                             >  1:3719669/1‑93 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGGAAATATGAGCGTAGACCCCTCCTTACTCCATGc                                            >  1:4172036/1‑93 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGc                                            <  1:5709488/93‑1 (MQ=255)
                                               ttGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCa                                           <  2:4465194/93‑1 (MQ=255)
                                               ctGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCa                                           <  2:1264763/92‑1 (MQ=255)
                                                 gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaa                                         <  1:2201786/93‑1 (MQ=255)
                                                 gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaa                                         <  1:1893802/93‑1 (MQ=255)
                                                  ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGACCCCTACTTACTCGATGCaaaa                                        >  1:4887561/1‑93 (MQ=255)
                                                   cgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAg                                       <  2:1018142/93‑1 (MQ=255)
                                                   cgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAg                                       >  2:6644999/1‑93 (MQ=255)
                                                     cccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCa                                     <  2:2316/93‑1 (MQ=255)
                                                     cccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAACa                                     >  2:1994907/1‑93 (MQ=255)
                                                      ccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGACCCCTACTTACTCGATGCAAAAGCAc                                    >  1:2594681/1‑93 (MQ=255)
                                                        aGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                  <  2:6264330/93‑1 (MQ=255)
                                                        aGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                  <  1:6685433/93‑1 (MQ=255)
                                                         ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACCct                                 >  2:4165549/1‑93 (MQ=255)
                                                          gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                <  2:3899931/93‑1 (MQ=255)
                                                                 aggcaggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGGAAAAGCACGCTCTGGTTAt                         >  2:4259917/1‑93 (MQ=255)
                                                                  ggcaggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                        <  2:6330867/93‑1 (MQ=255)
                                                                  ggcaggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                        >  1:414071/1‑93 (MQ=255)
                                                                    caggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGt                      <  2:1668053/93‑1 (MQ=255)
                                                                     aggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                     <  2:2026299/93‑1 (MQ=255)
                                                                     aggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                     <  2:4887693/93‑1 (MQ=255)
                                                                     aggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                     <  1:5568697/93‑1 (MQ=255)
                                                                     aggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                     <  2:5109127/93‑1 (MQ=255)
                                                                     aggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                     >  1:5207172/1‑93 (MQ=255)
                                                                      ggTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCt                    <  2:281229/93‑1 (MQ=255)
                                                                       gTCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGGACGCTCTGGTTATCGTCtt                   <  1:6474143/93‑1 (MQ=255)
                                                                        tCAGGCGCAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTg                  <  1:2942649/93‑1 (MQ=255)
                                                                            gcgcAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga              <  1:2448711/93‑1 (MQ=255)
                                                                            gcgcAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga              <  1:6475707/93‑1 (MQ=255)
                                                                            gcgcAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga              <  2:5327189/93‑1 (MQ=255)
                                                                             cgcAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAc             <  1:4413438/93‑1 (MQ=255)
                                                                              gcAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg            <  2:2800685/93‑1 (MQ=255)
                                                                              gcAGAGCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGTGACg            >  1:1437095/1‑93 (MQ=255)
                                                                               cAGAGCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACgg           <  1:6632112/93‑1 (MQ=255)
                                                                                agagCATTGTGGCGGGG‑GGGCATGGAAAATATGAGTTTAGCCCCCTACTTACTCGACGCAAAAGCCCGCTCTGGTTATCGTCTTGAAGACGGa          >  2:5528460/1‑93 (MQ=255)
                                                                                 gagCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc         <  1:5053479/93‑1 (MQ=255)
                                                                                 gagCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGAc         >  1:3997251/1‑93 (MQ=255)
                                                                                 gagCATTGTGGCGGGG‑GGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAAGCTCTGGTTATCGTCTTGGAGAAGGAc         >  1:3568515/1‑93 (MQ=255)
                                                                                 gagCATTGTGGCGGGG‑GGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACgggc         >  1:4165220/1‑91 (MQ=255)
                                                                                   gCATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAg       <  2:5995181/93‑1 (MQ=255)
                                                                                    cATTGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAgg      <  2:4539932/93‑1 (MQ=255)
                                                                                     aTTGTGGCGGGG‑GGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGt     >  2:4744676/1‑93 (MQ=255)
                                                                                      ttGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGtt    <  1:2156531/93‑1 (MQ=255)
                                                                                      ttGTGGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGtt    <  1:1660433/93‑1 (MQ=255)
                                                                                       tgtgGCGGGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGttt   <  2:5241095/93‑1 (MQ=255)
                                                                                             |                                                                                        
ATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324371‑2324550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324376‑2324562
                                                                                               |                                                                                              
CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGG                                                                                                <  SRR4302536.3486008/94‑1 (MQ=60)
CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGG                                                                                                <  SRR4302536.292881/94‑1 (MQ=60)
 GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGG‑G                                                                                              <  SRR4302536.6437575/94‑1 (MQ=60)
 GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGG‑G                                                                                              <  SRR4302536.3586369/94‑1 (MQ=60)
  GTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgg                                                                                              >  SRR4302536.3400461/1‑92 (MQ=60)
   TCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgga                                                                                             >  SRR4302536.4087359/1‑91 (MQ=60)
   TCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTCCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgta                                                                                             <  SRR4302536.1287695/94‑4 (MQ=60)
    CAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgggt                                                                                            >  SRR4302536.104699/1‑90 (MQ=60)
     AATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATG                                                                                           <  SRR4302536.167398/94‑1 (MQ=60)
     AATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGggatg                                                                                           >  SRR4302536.1260719/1‑89 (MQ=60)
       TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgggtggg                                                                                         >  SRR4302536.1227256/1‑87 (MQ=60)
       TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGggaatgg                                                                                         >  SRR4302536.995049/1‑87 (MQ=60)
         AAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAA                                                                                       <  SRR4302536.1508395/94‑1 (MQ=60)
         AAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAA                                                                                       <  SRR4302536.2290351/94‑1 (MQ=60)
          AGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAA                                                                                      >  SRR4302536.4363579/1‑94 (MQ=60)
          AGTATGTGGTTCGGGGCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGGGGC‑GGG‑GGGgggagggaaa                                                                                      >  SRR4302536.627710/1‑84 (MQ=60)
           GTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGggatggcaaaa                                                                                     >  SRR4302536.101443/1‑83 (MQ=60)
           GTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCAAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAAT                                                                                     <  SRR4302536.4372965/94‑1 (MQ=60)
              TGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATG                                                                                  <  SRR4302536.531755/94‑1 (MQ=60)
               GTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgggtggggaatatgg                                                                                 >  SRR4302536.182426/1‑79 (MQ=60)
                TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGTGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAG                                                                                <  SRR4302536.4925485/94‑1 (MQ=60)
                TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAG                                                                                <  SRR4302536.6520935/94‑1 (MQ=60)
                TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgggtgggaaataagag                                                                                >  SRR4302536.6222675/1‑78 (MQ=60)
                TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGggatgggaaaaatgag                                                                                >  SRR4302536.1528229/1‑78 (MQ=60)
                 GTTTCGGTTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGT                                                                               <  SRR4302536.5902932/94‑1 (MQ=60)
                 GCTTCGGGTCTTAAAACTGTGCCACTTGCCGCCCAGGCAAGTCAGGCAGGTCAGGGGCAGAGCTTTGTGGCGGGG‑GGG‑TATGGAAAATATGAGT                                                                               <  SRR4302536.3525622/94‑1 (MQ=53)
                    TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGgggtgggaaatattagtttt                                                                            >  SRR4302536.6023455/1‑74 (MQ=60)
                    TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCACGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGGTTA                                                                            >  SRR4302536.310124/1‑94 (MQ=60)
                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAG                                                                           <  SRR4302536.3098140/94‑1 (MQ=60)
                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAG                                                                           <  SRR4302536.528439/94‑1 (MQ=60)
                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAG                                                                           <  SRR4302536.5838705/94‑1 (MQ=60)
                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAG                                                                           >  SRR4302536.1063704/1‑94 (MQ=60)
                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAG                                                                           >  SRR4302536.1183485/1‑94 (MQ=60)
                     CGGGTCTGAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAG                                                                           <  SRR4302536.5164436/94‑1 (MQ=60)
                      GGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGC                                                                          <  SRR4302536.1581625/94‑1 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCC                                                                        <  SRR4302536.3167406/94‑1 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCC                                                                        <  SRR4302536.2260681/94‑1 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATTGAAAATATGAAGTTAGCCC                                                                        >  SRR4302536.3373348/1‑94 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGTTTAGCCC                                                                        >  SRR4302536.5982573/1‑94 (MQ=60)
                         TCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCC                                                                       <  SRR4302536.3122705/94‑1 (MQ=60)
                         TCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGTTTAGCCCC                                                                       >  SRR4302536.379340/1‑94 (MQ=60)
                          CTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGTTTAGACCCC                                                                      >  SRR4302536.5852819/1‑94 (MQ=60)
                          CTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCC                                                                      >  SRR4302536.3455378/1‑94 (MQ=60)
                           TTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCT                                                                     <  SRR4302536.2095439/94‑1 (MQ=60)
                            TAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TGTGGAAAATATGAGTTTtggcccccc                                                                    >  SRR4302536.1909332/1‑85 (MQ=60)
                             AAAAGTGTGGCGCTTGCCGCCGAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGGTGGG‑TATGGAAAATATGAGTTTAGCCCCCTAC                                                                   <  SRR4302536.2882875/94‑1 (MQ=60)
                             AAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GGTGGGAAATATGAGTTTTGCCCCCTAC                                                                   >  SRR4302536.4092766/1‑94 (MQ=60)
                             AAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGGTTAGCCCCCTAC                                                                   >  SRR4302536.1040603/1‑94 (MQ=60)
                              AAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACT                                                                  <  SRR4302536.4994283/94‑1 (MQ=60)
                              AAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGGTTAGCACCCTCCT                                                                  >  SRR4302536.5777071/1‑94 (MQ=60)
                              AAAGTGTGGCGCTTGCCGCCCAGGCCATGCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCCACT                                                                  <  SRR4302536.3633021/94‑1 (MQ=60)
                                AGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTA                                                                <  SRR4302536.4290058/94‑1 (MQ=60)
                                      GCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGAT                                                          <  SRR4302536.486513/94‑1 (MQ=60)
                                      GCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGAT                                                          <  SRR4302536.4485921/94‑1 (MQ=60)
                                      GCGCCTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTCCTTACTCGAT                                                          <  SRR4302536.3043102/94‑1 (MQ=60)
                                       CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATG                                                         <  SRR4302536.5573288/94‑1 (MQ=60)
                                       CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATG                                                         >  SRR4302536.3830858/1‑94 (MQ=60)
                                        GCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGGAAATATGAGCGTAGACCCCTCCTTACTCCATGC                                                        >  SRR4302536.4295976/1‑94 (MQ=60)
                                        GCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTTGCCCCCTACTTACTCGATGC                                                        <  SRR4302536.6755046/94‑1 (MQ=60)
                                         CTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCA                                                       <  SRR4302536.1065646/94‑1 (MQ=60)
                                         CTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCA                                                       <  SRR4302536.5879282/94‑1 (MQ=60)
                                         CTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCA                                                       >  SRR4302536.5514171/1‑94 (MQ=60)
                                            GCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAA                                                    <  SRR4302536.1210286/94‑1 (MQ=60)
                                            GCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAA                                                    <  SRR4302536.1950774/94‑1 (MQ=60)
                                            GCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAA                                                    <  SRR4302536.2268254/94‑1 (MQ=60)
                                            GCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGACCCCTACTTACTCGATGCAAAA                                                    >  SRR4302536.5034579/1‑94 (MQ=60)
                                              CGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGC                                                  <  SRR4302536.533028/94‑1 (MQ=60)
                                              CGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGC                                                  >  SRR4302536.2561996/1‑94 (MQ=60)
                                                CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAC                                                <  SRR4302536.6678994/94‑1 (MQ=60)
                                                CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAC                                                <  SRR4302536.574859/94‑1 (MQ=60)
                                                CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACAC                                                >  SRR4302536.3657461/1‑94 (MQ=60)
                                                CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGACCCCTACTTACTCGATGCAAAAGCAC                                                >  SRR4302536.2672589/1‑94 (MQ=60)
                                                 CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACG                                               >  SRR4302536.1695919/1‑94 (MQ=60)
                                                  CAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATGTGAGTTTAGCCCCCTACTTTCTCGATtccacaacacac                                              >  SRR4302536.3859234/1‑82 (MQ=60)
                                                  CAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGATTAGCCCCCTACTTACTTGATGCAAAAGCACGC                                              >  SRR4302536.1695511/1‑94 (MQ=60)
                                                   AGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCT                                             <  SRR4302536.6883991/94‑1 (MQ=60)
                                                   AGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCT                                             >  SRR4302536.3692056/1‑94 (MQ=60)
                                                   AGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCT                                             >  SRR4302536.5326757/1‑94 (MQ=60)
                                                   AGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGGTTAGCCCCCTACTTACTCCATGCAAAAcgacgcc                                             >  SRR4302536.2120414/1‑87 (MQ=60)
                                                    GGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGAAAGCTC                                            >  SRR4302536.5361693/1‑94 (MQ=60)
                                                     GCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCT                                           <  SRR4302536.155226/94‑1 (MQ=60)
                                                      CCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCCCCTTACTCGATGCAAAAGAACGCTCTG                                          >  SRR4302536.1981080/1‑94 (MQ=60)
                                                       CATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TGTGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         >  SRR4302536.5240762/1‑94 (MQ=60)
                                                       CATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         >  SRR4302536.6841979/1‑94 (MQ=60)
                                                         TTCAGGCGGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGGATATGTAAAATATGAGTTTAGCCCCCTGCTTACTCGATGCAAAAGCACGCTCTGGTT                                       <  SRR4302536.6174674/94‑1 (MQ=60)
                                                         TTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTT                                       <  SRR4302536.2081273/94‑1 (MQ=60)
                                                            AGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC                                    >  SRR4302536.424998/1‑94 (MQ=60)
                                                            AGGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC                                    >  SRR4302536.4153321/1‑94 (MQ=60)
                                                             GGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCG                                   <  SRR4302536.5593089/94‑1 (MQ=60)
                                                             GGCAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTAACG                                   <  SRR4302536.6605159/94‑1 (MQ=60)
                                                               CAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATTGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCcctgggtttccgc                                 >  SRR4302536.5849705/1‑81 (MQ=60)
                                                               CAGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTC                                 >  SRR4302536.5362383/1‑94 (MQ=60)
                                                                AGGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCT                                <  SRR4302536.5734486/94‑1 (MQ=60)
                                                                 GGTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTT                               >  SRR4302536.2093924/1‑94 (MQ=60)
                                                                  GTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGTACGCTCTGGTTATCGTCTTG                              <  SRR4302536.6667355/94‑1 (MQ=60)
                                                                  GTCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGGACGCTCTGGTTATCGTCTTG                              <  SRR4302536.6667354/94‑1 (MQ=60)
                                                                   TCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGG                             <  SRR4302536.3029412/94‑1 (MQ=60)
                                                                   TCAGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGG                             <  SRR4302536.3029411/94‑1 (MQ=60)
                                                                     AGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGGG                           >  SRR4302536.2786211/1‑94 (MQ=60)
                                                                     AGGCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG                           <  SRR4302536.3711410/94‑1 (MQ=60)
                                                                       GCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAC                         <  SRR4302536.2522689/94‑1 (MQ=60)
                                                                       GCGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAC                         <  SRR4302536.6668938/94‑1 (MQ=60)
                                                                        CGCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACG                        <  SRR4302536.4545520/94‑1 (MQ=60)
                                                                        CGCAGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGTGACG                        >  SRR4302536.1479732/1‑94 (MQ=60)
                                                                         GCAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGG                       <  SRR4302536.1671253/94‑1 (MQ=60)
                                                                          CAGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGA                      <  SRR4302536.6829385/94‑1 (MQ=60)
                                                                           AGAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC                     <  SRR4302536.3989278/94‑1 (MQ=60)
                                                                           AGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGAC                     >  SRR4302536.4115866/1‑94 (MQ=60)
                                                                           AGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAAGCTCTGGTTATCGTCTTGGAGAAGGAC                     >  SRR4302536.3675204/1‑94 (MQ=60)
                                                                           AGAGCATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGGC                     >  SRR4302536.4288968/1‑94 (MQ=60)
                                                                            GAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA                    <  SRR4302536.6454379/94‑1 (MQ=60)
                                                                            GAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA                    <  SRR4302536.1410173/94‑1 (MQ=60)
                                                                            GAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA                    <  SRR4302536.1736698/94‑1 (MQ=60)
                                                                            GAGCATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA                    <  SRR4302536.5204879/94‑1 (MQ=60)
                                                                            GAGCATTGTGGCGGGG‑GGG‑GATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATTGTCTTGGAGACGGACA                    >  SRR4302536.2041258/1‑94 (MQ=60)
                                                                               CATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCCACTTACTCCATGCAAAAGCACGCTCTGGTTATCCTCTTGGGGACGGGCACGT                 >  SRR4302536.1364464/1‑94 (MQ=60)
                                                                                ATTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTT                <  SRR4302536.810320/94‑1 (MQ=60)
                                                                                ATTGTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGACAGGGT                >  SRR4302536.2634141/1‑94 (MQ=60)
                                                                                 TTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTT               <  SRR4302536.4270834/94‑1 (MQ=60)
                                                                                 TTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTT               <  SRR4302536.1708633/94‑1 (MQ=60)
                                                                                 TTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTT               <  SRR4302536.494991/94‑1 (MQ=60)
                                                                                 TTGTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTT               <  SRR4302536.2221362/94‑1 (MQ=60)
                                                                                   GTGGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAT             <  SRR4302536.412366/94‑1 (MQ=60)
                                                                                   GTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAT             >  SRR4302536.5656774/1‑94 (MQ=60)
                                                                                   GTGGCGGGG‑GGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAT             >  SRR4302536.547041/1‑94 (MQ=60)
                                                                                     GGCGGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGA           <  SRR4302536.6547195/94‑1 (MQ=60)
                                                                                      gcgGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGAC          <  SRR4302536.4834821/91‑1 (MQ=60)
                                                                                       cgGGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACG         <  SRR4302536.4005223/92‑1 (MQ=60)
                                                                                           G‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAAT     >  SRR4302536.5452529/1‑94 (MQ=60)
                                                                                           G‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAAT     >  SRR4302536.6252912/1‑94 (MQ=60)
                                                                                              GG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCC   <  SRR4302536.43343/94‑1 (MQ=60)
                                                                                               G‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  <  SRR4302536.4746672/94‑1 (MQ=60)
                                                                                               |                                                                                              
CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGG‑GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324376‑2324562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑