Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,345,161 T→C C759C (TGT→TGC)  nrdA → ribonucleoside diphosphate reductase 1 alpha subunit, B1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,345,1610TC96.8% 488.5 / 2.2 156C759C (TGT→TGC) nrdAribonucleoside diphosphate reductase 1 alpha subunit, B1
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (71/80);  total (74/82)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 159 total aligned reads displayed.

GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250
                                                                                          |                                                                                         
gTATTATCAGAACACCCGTGGCGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCaa                                                                                         <  1:2898538/93‑1 (MQ=255)
gTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCaa                                                                                         <  1:843177/93‑1 (MQ=255)
  attatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGa                                                                                       >  2:4288160/1‑93 (MQ=255)
   ttatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGAt                                                                                      >  2:1571344/1‑93 (MQ=255)
   ttatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGAt                                                                                      <  1:3091054/93‑1 (MQ=255)
    tatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATc                                                                                     >  1:2940207/1‑93 (MQ=255)
     atCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCt                                                                                    <  1:1673438/93‑1 (MQ=255)
      tCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTg                                                                                   <  1:4333057/93‑1 (MQ=255)
      tCAGAACACCCGTGACGGCGCTGAAGACCCACAAGACGATCTGGTGCCGTCAATCCAGAACGATGGCTGCGAAAGCGGCGCATGCAAGATCTg                                                                                   >  2:3939759/1‑93 (MQ=255)
       cAGAACACCCGTGACGGCGCTGAAGACGCACAATACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGa                                                                                  <  1:4567496/93‑1 (MQ=255)
       cAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGa                                                                                  <  2:651265/93‑1 (MQ=255)
        aGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGat                                                                                 <  1:4516673/93‑1 (MQ=255)
        aGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGat                                                                                 <  2:4469282/93‑1 (MQ=255)
         gAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGata                                                                                >  1:2376467/1‑93 (MQ=255)
         gAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGata                                                                                <  1:1969551/93‑1 (MQ=255)
           acacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAtt                                                                              <  1:4182938/93‑1 (MQ=255)
             acCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTga                                                                            >  2:1263324/1‑93 (MQ=255)
             acCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTga                                                                            >  1:1176049/1‑93 (MQ=255)
               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  2:4020106/93‑1 (MQ=255)
               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  2:220795/93‑1 (MQ=255)
                cGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAt                                                                         <  1:2559096/93‑1 (MQ=255)
                cGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAt                                                                         <  1:3693163/93‑1 (MQ=255)
                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       <  1:461166/93‑1 (MQ=255)
                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       <  1:2424046/93‑1 (MQ=255)
                   gACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGcc                                                                      <  2:2355071/93‑1 (MQ=255)
                      ggCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGa                                                                   <  1:4338349/93‑1 (MQ=255)
                       gcgcGGAAGACCCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAt                                                                  <  2:1971016/93‑1 (MQ=255)
                         gcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgc                                                                <  1:2543858/93‑1 (MQ=255)
                         gcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgc                                                                <  2:2337507/93‑1 (MQ=255)
                         gcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgc                                                                <  2:2456441/93‑1 (MQ=255)
                          cTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcg                                                               <  1:2046484/93‑1 (MQ=255)
                           tGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcgg                                                              >  1:2710007/1‑93 (MQ=255)
                            gAAGCCGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             <  2:3411637/93‑1 (MQ=255)
                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             >  2:4525885/1‑93 (MQ=255)
                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             <  1:2085319/93‑1 (MQ=255)
                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             >  2:1214371/1‑93 (MQ=255)
                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        >  2:4524435/1‑93 (MQ=255)
                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        <  2:781467/93‑1 (MQ=255)
                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        >  1:1852381/1‑93 (MQ=255)
                                  gCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAc                                                       <  2:2986222/93‑1 (MQ=255)
                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    >  1:2773139/1‑93 (MQ=255)
                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    >  1:4458423/1‑93 (MQ=255)
                                       aGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCtt                                                  >  1:1512856/1‑93 (MQ=255)
                                        gACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTa                                                 <  2:1302306/93‑1 (MQ=255)
                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                <  2:256882/93‑1 (MQ=255)
                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                >  1:2511913/1‑93 (MQ=255)
                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                <  2:3574955/93‑1 (MQ=255)
                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                <  1:2413666/93‑1 (MQ=255)
                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  2:1898568/93‑1 (MQ=255)
                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  2:1715060/93‑1 (MQ=255)
                                           gATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATcc                                              >  1:289740/1‑93 (MQ=255)
                                           gATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATcc                                              <  2:2255557/93‑1 (MQ=255)
                                           gATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATcc                                              >  2:2888945/1‑93 (MQ=255)
                                            aTCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCg                                             >  1:799299/1‑93 (MQ=255)
                                            aTCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCg                                             >  2:2640407/1‑93 (MQ=255)
                                            aTCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCg                                             <  1:3878007/93‑1 (MQ=255)
                                               tGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                          <  2:3823921/93‑1 (MQ=255)
                                               tGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                          <  1:3204293/93‑1 (MQ=255)
                                               tGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                          <  2:1323841/93‑1 (MQ=255)
                                                 gTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                        <  1:2650081/93‑1 (MQ=255)
                                                  tGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                       >  2:1840354/1‑93 (MQ=255)
                                                   gCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                      <  2:3574239/93‑1 (MQ=255)
                                                   gCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                      <  2:2424368/93‑1 (MQ=255)
                                                      ggcAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCt                                   <  2:4380638/91‑1 (MQ=255)
                                                      gTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCt                                   >  2:580556/1‑93 (MQ=255)
                                                      gTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCt                                   >  2:2256649/1‑93 (MQ=255)
                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTc                                  >  1:4692276/1‑93 (MQ=255)
                                                        cAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCg                                 >  1:3476521/1‑93 (MQ=255)
                                                        cAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGAGGCGTAAACGCCTTATCCGGCCTACGGCTCg                                 >  2:615163/1‑93 (MQ=255)
                                                         aaTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCgg                                <  1:1154849/93‑1 (MQ=255)
                                                          aTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGt                               >  2:462406/1‑93 (MQ=255)
                                                          aTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGt                               >  2:2483336/1‑93 (MQ=255)
                                                           tCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGtt                              <  2:808875/93‑1 (MQ=255)
                                                            ccAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGttt                             <  1:3321455/93‑1 (MQ=255)
                                                            ccAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGttt                             >  1:2409763/1‑93 (MQ=255)
                                                            ccAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGttt                             <  1:4000709/93‑1 (MQ=255)
                                                              aGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGt                           >  1:646650/1‑93 (MQ=255)
                                                               ggACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTa                          >  2:1982522/1‑93 (MQ=255)
                                                               ggACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTa                          >  1:1984070/1‑93 (MQ=255)
                                                                  cGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGc                       <  1:822380/93‑1 (MQ=255)
                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGcc                      <  2:2959018/93‑1 (MQ=255)
                                                                    aTGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCt                     <  2:366131/93‑1 (MQ=255)
                                                                      ggCTGCGAAAGCGGGGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGa                   <  2:4064767/93‑1 (MQ=255)
                                                                      ggCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGa                   >  1:3991789/1‑93 (MQ=255)
                                                                       gCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAt                  >  2:1133841/1‑93 (MQ=255)
                                                                         tGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATaa                <  1:1802822/93‑1 (MQ=255)
                                                                          gCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAg               <  1:2370574/93‑1 (MQ=255)
                                                                           cGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGa              >  2:1723033/1‑93 (MQ=255)
                                                                            gAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAc             >  2:2168445/1‑93 (MQ=255)
                                                                            gAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAc             >  1:1605135/1‑93 (MQ=255)
                                                                             aaaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcg            >  2:3162255/1‑93 (MQ=255)
                                                                             aaaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcg            <  1:1307173/93‑1 (MQ=255)
                                                                               aGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcgcg          >  1:2493950/1‑93 (MQ=255)
                                                                                 cggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGcc        <  2:3669214/93‑1 (MQ=255)
                                                                                 cggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGcc        <  1:2075704/93‑1 (MQ=255)
                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCa       <  2:1881387/93‑1 (MQ=255)
                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTCATAAGACGCGCCa       >  1:3929206/1‑93 (MQ=255)
                                                                                     gcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCg    >  2:3500562/1‑93 (MQ=255)
                                                                                       aTGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTc  >  2:4178441/1‑93 (MQ=255)
                                                                                       aTGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTc  >  1:1733528/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345070‑2345254
                                                                                           |                                                                                             
TGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAA                                                                                             >  SRR4302544.3840030/1‑94 (MQ=60)
 GTATTATCAGAACACCCGTGGCGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            <  SRR4302544.2926455/94‑1 (MQ=60)
 GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            <  SRR4302544.851506/94‑1 (MQ=60)
    TTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATC                                                                                         >  SRR4302544.2968444/1‑94 (MQ=60)
    TTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATC                                                                                         <  SRR4302544.3120501/94‑1 (MQ=60)
      ATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTG                                                                                       >  SRR4302544.2834793/1‑94 (MQ=60)
      ATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTG                                                                                       <  SRR4302544.1689923/94‑1 (MQ=60)
       TCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGA                                                                                      <  SRR4302544.4377171/94‑1 (MQ=60)
        CAGAACACCCGTGACGGCGCTGAAGACGCACAATACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     <  SRR4302544.4613032/94‑1 (MQ=60)
         AGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATA                                                                                    <  SRR4302544.2045350/94‑1 (MQ=60)
         AGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATA                                                                                    >  SRR4302544.2398967/1‑94 (MQ=60)
         AGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATA                                                                                    <  SRR4302544.4561904/94‑1 (MQ=60)
          aaaccCCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAT                                                                                   <  SRR4302544.1559340/89‑1 (MQ=60)
          GAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAT                                                                                   <  SRR4302544.1988681/94‑1 (MQ=60)
            ACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTG                                                                                 <  SRR4302544.4224362/94‑1 (MQ=60)
             CACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGA                                                                                >  SRR4302544.4417865/1‑94 (MQ=60)
             CACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGA                                                                                >  SRR4302544.1187775/1‑94 (MQ=60)
              ACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAG                                                                               >  SRR4302544.2415587/1‑94 (MQ=60)
              ACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAG                                                                               <  SRR4302544.4769060/94‑1 (MQ=60)
               CCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGA                                                                              >  SRR4302544.1705991/1‑94 (MQ=60)
                CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             >  SRR4302544.3642548/1‑94 (MQ=60)
                CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             >  SRR4302544.2706884/1‑94 (MQ=60)
                 CGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATG                                                                            <  SRR4302544.3729959/94‑1 (MQ=60)
                 CGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATG                                                                            <  SRR4302544.2583675/94‑1 (MQ=60)
                  GTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGC                                                                           >  SRR4302544.2955669/1‑94 (MQ=60)
                   TGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCC                                                                          <  SRR4302544.465699/94‑1 (MQ=60)
                   TGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCC                                                                          <  SRR4302544.2447119/94‑1 (MQ=60)
                    GACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCG                                                                         <  SRR4302544.216480/94‑1 (MQ=60)
                       GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      <  SRR4302544.4382500/94‑1 (MQ=60)
                          GCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCG                                                                   <  SRR4302544.2568279/94‑1 (MQ=60)
                           CTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGG                                                                  <  SRR4302544.2066208/94‑1 (MQ=60)
                           CTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGG                                                                  >  SRR4302544.4342540/1‑94 (MQ=60)
                           CTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGG                                                                  >  SRR4302544.2736070/1‑94 (MQ=60)
                            TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATCCCCGATGCGGC                                                                 >  SRR4302544.2548453/1‑94 (MQ=60)
                             GAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCG                                                                <  SRR4302544.2105374/94‑1 (MQ=60)
                                 ACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAA                                                            >  SRR4302544.1870302/1‑94 (MQ=60)
                                    CACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGC                                                         >  SRR4302544.1762948/1‑94 (MQ=60)
                                     ACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCC                                                        >  SRR4302544.4503308/1‑94 (MQ=60)
                                     ACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCC                                                        >  SRR4302544.2799810/1‑94 (MQ=60)
                                      CAAGACGATCTGGTGCCGTAAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCT                                                       >  SRR4302544.4675065/1‑94 (MQ=60)
                                       AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      >  SRR4302544.1527848/1‑94 (MQ=60)
                                        AGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTA                                                     <  SRR4302544.2635877/94‑1 (MQ=60)
                                         GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAT                                                    >  SRR4302544.2536021/1‑94 (MQ=60)
                                          ACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATC                                                   <  SRR4302544.2436623/94‑1 (MQ=60)
                                           CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  >  SRR4302544.292739/1‑94 (MQ=60)
                                            GATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCG                                                 <  SRR4302544.2463823/94‑1 (MQ=60)
                                            GATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCG                                                 >  SRR4302544.807049/1‑94 (MQ=60)
                                             ATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGG                                                <  SRR4302544.3916509/94‑1 (MQ=60)
                                                TGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCT                                             <  SRR4302544.3000067/94‑1 (MQ=60)
                                                TGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCT                                             <  SRR4302544.3234845/94‑1 (MQ=60)
                                                  GTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC                                           <  SRR4302544.2675498/94‑1 (MQ=60)
                                                   TGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACG                                          <  SRR4302544.193487/94‑1 (MQ=60)
                                                       GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302544.4738623/1‑94 (MQ=60)
                                                        TCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCG                                     >  SRR4302544.3509673/1‑94 (MQ=60)
                                                        TCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCG                                     >  SRR4302544.4120378/1‑94 (MQ=60)
                                                          AATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGT                                   <  SRR4302544.1166337/94‑1 (MQ=60)
                                                           ATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCcgct                                  >  SRR4302544.3999914/1‑90 (MQ=60)
                                                            TCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTT                                 >  SRR4302544.2432663/1‑94 (MQ=60)
                                                            TCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTGT                                 >  SRR4302544.2432664/1‑94 (MQ=60)
                                                             CCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTG                                <  SRR4302544.3353095/94‑1 (MQ=60)
                                                             CCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTG                                <  SRR4302544.4040529/94‑1 (MQ=60)
                                                              CAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGT                               >  SRR4302544.653037/1‑94 (MQ=60)
                                                               AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              >  SRR4302544.2003336/1‑94 (MQ=60)
                                                                  ACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGC                           <  SRR4302544.2085084/94‑1 (MQ=60)
                                                                   CGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCC                          <  SRR4302544.830419/94‑1 (MQ=60)
                                                                      TGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGA                       >  SRR4302544.4031526/1‑94 (MQ=60)
                                                                       GGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGGCTGAT                      >  SRR4302544.119174/1‑94 (MQ=60)
                                                                          TGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAG                   <  SRR4302544.1820435/94‑1 (MQ=60)
                                                                          TGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAG                   <  SRR4302544.4608292/94‑1 (MQ=60)
                                                                           GCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGA                  <  SRR4302544.2393006/94‑1 (MQ=60)
                                                                            CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 >  SRR4302544.1620961/1‑94 (MQ=60)
                                                                              AAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               <  SRR4302544.1320026/94‑1 (MQ=60)
                                                                               AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCG              >  SRR4302544.2517844/1‑94 (MQ=60)
                                                                                AGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGC             >  SRR4302544.883888/1‑94 (MQ=60)
                                                                                  CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           <  SRR4302544.2095651/94‑1 (MQ=60)
                                                                                  CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTCATAAGACGCGCCA           >  SRR4302544.3968226/1‑94 (MQ=60)
                                                                                       CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      >  SRR4302544.1750519/1‑94 (MQ=60)
                                                                                       CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      >  SRR4302544.3277056/1‑94 (MQ=60)
                                                                                       CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      <  SRR4302544.807875/94‑1 (MQ=60)
                                                                                         TGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGC    >  SRR4302544.383253/1‑94 (MQ=60)
                                                                                           CAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAAACGCGCCAGCGTCacac  >  SRR4302544.980696/1‑90 (MQ=60)
                                                                                           CAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  SRR4302544.980695/1‑94 (MQ=60)
                                                                                           |                                                                                             
TGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345070‑2345254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑