Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,345,161	T→C	C759C (TGT→TGC)	nrdA →	ribonucleoside diphosphate reductase 1 alpha subunit, B1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,345,161	0	T	C	100.0%	913.2 / NA	273	C759C (TGT→TGC)	nrdA	ribonucleoside diphosphate reductase 1 alpha subunit, B1
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (134/139); total (134/139)

Only 100 of 277 total aligned reads displayed.

GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250

gTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCaa                                                                                         <  1:2185246/93‑1 (MQ=255)

    tatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATc                                                                                     >  2:6482166/1‑93 (MQ=255)

       cAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGa                                                                                  <  2:2847798/93‑1 (MQ=255)

         gAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGata                                                                                >  2:4970682/1‑93 (MQ=255)

         gAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGata                                                                                >  2:2241430/1‑93 (MQ=255)

          aaCACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGatat                                                                               >  1:5938049/1‑93 (MQ=255)

           acacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAtt                                                                              >  1:2122622/1‑93 (MQ=255)

           acacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAtt                                                                              <  1:883014/93‑1 (MQ=255)

           acacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAtt                                                                              >  2:5128605/1‑93 (MQ=255)

           acacCCGTGACGGCGCTAAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAtt                                                                              <  1:648993/93‑1 (MQ=255)

            cacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTg                                                                             <  2:3524925/93‑1 (MQ=255)

             acCCGTGACGCCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAttca                                                                            <  1:3468636/93‑3 (MQ=255)

              cccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgag                                                                           >  2:3807740/1‑93 (MQ=255)

              cccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgag                                                                           <  1:5193158/93‑1 (MQ=255)

              cccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgag                                                                           >  1:3179596/1‑93 (MQ=255)

                cGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAt                                                                         <  1:5062486/93‑1 (MQ=255)

                 gTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATg                                                                        <  2:3681163/93‑1 (MQ=255)

                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       <  2:1356169/93‑1 (MQ=255)

                     cGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCgg                                                                    >  1:458061/1‑93 (MQ=255)

                      ggCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGa                                                                   <  2:5647385/93‑1 (MQ=255)

                      ggCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGa                                                                   <  2:6300603/93‑1 (MQ=255)

                       gcgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAt                                                                  <  2:4052840/93‑1 (MQ=255)

                       gcgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAt                                                                  <  1:4361335/93‑1 (MQ=255)

                        cgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATg                                                                 >  1:3071017/1‑93 (MQ=255)

                        cgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATg                                                                 <  2:2165792/93‑1 (MQ=255)

                        cgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATg                                                                 <  1:998415/93‑1 (MQ=255)

                          cTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcg                                                               <  2:3055782/93‑1 (MQ=255)

                           tGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGATGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcgg                                                              >  2:5413269/1‑93 (MQ=255)

                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             >  2:3458051/1‑93 (MQ=255)

                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             >  1:4435521/1‑93 (MQ=255)

                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             <  2:3336101/93‑1 (MQ=255)

                             aaGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggcg                                                            <  2:4554511/93‑1 (MQ=255)

                             aaGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggcg                                                            >  1:3885690/1‑93 (MQ=255)

                             aaGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggcg                                                            <  1:2558058/93‑1 (MQ=255)

                              aGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGt                                                           <  2:2992324/93‑1 (MQ=255)

                              aGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGt                                                           >  2:762638/1‑93 (MQ=255)

                              aGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGt                                                           >  2:241075/1‑93 (MQ=255)

                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaac                                                        >  2:1593046/1‑92 (MQ=255)

                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        <  2:2550669/93‑1 (MQ=255)

                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        <  2:2600196/93‑1 (MQ=255)

                                   cacaAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACg                                                      >  1:5003834/1‑93 (MQ=255)

                                    acaAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGc                                                     >  2:1409270/1‑93 (MQ=255)

                                      aaGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCt                                                   >  2:4849526/1‑93 (MQ=255)

                                      aaGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCt                                                   <  2:3582345/93‑1 (MQ=255)

                                       aGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCtt                                                  >  1:1065734/1‑93 (MQ=255)

                                       aGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCtt                                                  <  2:5186643/93‑1 (MQ=255)

                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  1:752317/93‑1 (MQ=255)

                                            aTCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCg                                             >  1:614560/1‑93 (MQ=255)

                                               tGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                          <  1:670185/93‑1 (MQ=255)

                                               tGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                          <  1:1678210/93‑1 (MQ=255)

                                                ggTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                         <  1:2475381/93‑1 (MQ=255)

                                                ggTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                         <  2:6378739/93‑1 (MQ=255)

                                                 gTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                        >  2:4447830/1‑93 (MQ=255)

                                                 gTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                        >  2:5352806/1‑93 (MQ=255)

                                                   gCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                      <  2:2353515/93‑1 (MQ=255)

                                                    ccGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgg                                     >  1:3002150/1‑93 (MQ=255)

                                                      gTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCt                                   <  1:5823617/93‑1 (MQ=255)

                                                      gTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCt                                   <  1:5675169/93‑1 (MQ=255)

                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTc                                  >  1:3377283/1‑93 (MQ=255)

                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTc                                  >  1:6065104/1‑93 (MQ=255)

                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTc                                  <  1:4406176/93‑1 (MQ=255)

                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTc                                  >  1:4776404/1‑93 (MQ=255)

                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTc                                  <  1:1319341/93‑1 (MQ=255)

                                                         aaTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCgg                                >  1:1100971/1‑93 (MQ=255)

                                                          aTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGt                               >  1:3281257/1‑93 (MQ=255)

                                                          aTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGt                               >  2:4236343/1‑93 (MQ=255)

                                                           tCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGtt                              <  2:4638220/93‑1 (MQ=255)

                                                           accAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGtt                              <  1:5452240/92‑1 (MQ=255)

                                                             cAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTg                            >  2:3128814/1‑93 (MQ=255)

                                                             cAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTg                            >  1:2526951/1‑93 (MQ=255)

                                                              aGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGt                           <  2:2710564/93‑1 (MQ=255)

                                                              aGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGt                           >  2:1886211/1‑93 (MQ=255)

                                                               ggACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTa                          >  1:3632180/1‑93 (MQ=255)

                                                               ggACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTa                          <  1:4860092/93‑1 (MQ=255)

                                                                 aCGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAgg                        >  1:5627915/1‑93 (MQ=255)

                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTTACCGGCCTACGGCTCGGTTTGTAggtc                      >  1:5390833/1‑91 (MQ=38)

                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTTTAGGcc                      >  1:2756193/1‑93 (MQ=255)

                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGcc                      <  1:6342557/93‑1 (MQ=255)

                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGcc                      <  1:4001351/93‑1 (MQ=255)

                                                                    aTGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCt                     <  2:5864194/93‑1 (MQ=255)

                                                                    aTGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAAGCCt                     >  1:2914965/1‑93 (MQ=255)

                                                                     tGGCTGCGAAGGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCC‑TATCCGGCCTACGGCTCGGTTTGTAGGCCTGa                   <  1:424526/93‑1 (MQ=37)

                                                                     tGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTg                    >  1:1472915/1‑93 (MQ=255)

                                                                      ggCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGa                   <  2:5742009/93‑1 (MQ=255)

                                                                       gCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAt                  >  2:5503759/1‑93 (MQ=255)

                                                                          gCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAg               <  1:1789680/93‑1 (MQ=255)

                                                                           cGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGa              <  2:6606125/93‑1 (MQ=255)

                                                                            gAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAc             >  2:4369410/1‑93 (MQ=255)

                                                                            gAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAc             <  2:6687599/93‑1 (MQ=255)

                                                                               aGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGGCTGATAAGAcgcg          >  1:4855480/1‑93 (MQ=255)

                                                                               aGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcgcg          >  2:489397/1‑93 (MQ=255)

                                                                               aGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAagcg          >  2:1176325/1‑93 (MQ=255)

                                                                                gcggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACgcgc         >  2:812695/1‑93 (MQ=255)

                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCa       >  1:3839359/1‑93 (MQ=255)

                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAAGACTGATAAGAAGCGCCa       >  1:2844979/1‑93 (MQ=21)

                                                                                   gcgcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAg      <  1:5154765/93‑1 (MQ=255)

                                                                                   gcgcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAg      <  2:2054664/93‑1 (MQ=255)

                                                                                    cgcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGc     <  1:6174941/93‑1 (MQ=255)

                                                                                       aTGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTc  >  2:4251146/1‑93 (MQ=255)

                                                                                       aTGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTc  >  2:4083860/1‑93 (MQ=255)

GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345069‑2345254

CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              >  SRR4302536.1279136/1‑94 (MQ=60)

CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              >  SRR4302536.2161728/1‑94 (MQ=60)

CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              <  SRR4302536.5627262/94‑1 (MQ=60)

  GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            >  SRR4302536.904756/1‑94 (MQ=60)

  GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            <  SRR4302536.6336828/94‑1 (MQ=60)

  GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            <  SRR4302536.2251119/94‑1 (MQ=60)

  GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            >  SRR4302536.5815984/1‑94 (MQ=60)

    ATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGAT                                                                                          >  SRR4302536.3002664/1‑94 (MQ=60)

         CAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     <  SRR4302536.6679634/94‑1 (MQ=60)

         CAGAACACCCGTGACGGCGCTGAAGACCCACAAGACGATCTGGTCCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     <  SRR4302536.508433/94‑1 (MQ=60)

          AGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATA                                                                                    <  SRR4302536.2819145/94‑1 (MQ=60)

           GAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAT                                                                                   >  SRR4302536.6114941/1‑94 (MQ=60)

           GAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAT                                                                                   >  SRR4302536.6384046/1‑94 (MQ=60)

            AACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCTGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATT                                                                                  <  SRR4302536.5601391/94‑1 (MQ=60)

            AACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATT                                                                                  <  SRR4302536.2223144/94‑1 (MQ=60)

            AACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATT                                                                                  >  SRR4302536.2186399/1‑94 (MQ=60)

            AACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATT                                                                                  >  SRR4302536.5031985/1‑94 (MQ=60)

             ACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTG                                                                                 <  SRR4302536.909001/94‑1 (MQ=60)

             ACACCCGTGACGGCGCTAAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTG                                                                                 <  SRR4302536.668066/94‑1 (MQ=60)

               CCCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAG                                                                               <  SRR4302536.1598826/94‑1 (MQ=60)

               ACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAG                                                                               >  SRR4302536.3272821/1‑94 (MQ=60)

               ACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAC                                                                               <  SRR4302536.3539433/94‑1 (MQ=60)

               ACCCGTGACGCCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTCAG                                                                               <  SRR4302536.3572018/94‑1 (MQ=60)

                CCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGA                                                                              <  SRR4302536.5348011/94‑1 (MQ=60)

                CCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATACAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGT                                                                              >  SRR4302536.3407590/1‑94 (MQ=60)

                 CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302536.6312599/94‑1 (MQ=60)

                  CGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATG                                                                            <  SRR4302536.4936756/94‑1 (MQ=60)

                  CGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATG                                                                            <  SRR4302536.5214120/94‑1 (MQ=60)

                  CGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATG                                                                            >  SRR4302536.4427691/1‑94 (MQ=60)

                    TGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCC                                                                          >  SRR4302536.5184598/1‑94 (MQ=60)

                     GACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCG                                                                         <  SRR4302536.1327676/94‑1 (MQ=60)

                      ACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGG                                                                        >  SRR4302536.470048/1‑94 (MQ=60)

                      ACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGG                                                                        <  SRR4302536.1784803/94‑1 (MQ=60)

                        GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      >  SRR4302536.3712435/1‑94 (MQ=60)

                        GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      >  SRR4302536.5551267/1‑94 (MQ=60)

                        GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      >  SRR4302536.5944330/1‑94 (MQ=60)

                         GCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATG                                                                     <  SRR4302536.4491706/94‑1 (MQ=60)

                         GCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATG                                                                     <  SRR4302536.3538506/94‑1 (MQ=60)

                         GCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATG                                                                     >  SRR4302536.3160997/1‑94 (MQ=60)

                          CGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGC                                                                    <  SRR4302536.1027867/94‑1 (MQ=60)

                          CGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGC                                                                    <  SRR4302536.2131064/94‑1 (MQ=60)

                           GCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCG                                                                   >  SRR4302536.4010575/1‑94 (MQ=60)

                            CTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGG                                                                  >  SRR4302536.6078799/1‑94 (MQ=60)

                            CTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGG                                                                  >  SRR4302536.2223686/1‑94 (MQ=60)

                             TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGC                                                                 >  SRR4302536.4568605/1‑94 (MQ=60)

                              GAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCG                                                                <  SRR4302536.6200780/94‑1 (MQ=60)

                              GAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCG                                                                >  SRR4302536.4001257/1‑94 (MQ=60)

                              GAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCG                                                                <  SRR4302536.3866442/94‑1 (MQ=60)

                               AAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGT                                                               <  SRR4302536.2635055/94‑1 (MQ=60)

                               AAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGT                                                               >  SRR4302536.3700642/1‑94 (MQ=60)

                                AGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTA                                                              <  SRR4302536.3196928/94‑1 (MQ=60)

                                    GCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACG                                                          <  SRR4302536.2951917/94‑1 (MQ=60)

                                    GCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACG                                                          >  SRR4302536.5153969/1‑94 (MQ=60)

                                     CACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGC                                                         >  SRR4302536.6826222/1‑94 (MQ=60)

                                     CACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGC                                                         >  SRR4302536.6884965/1‑94 (MQ=60)

                                     CACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGC                                                         >  SRR4302536.5313402/1‑94 (MQ=60)

                                        AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      <  SRR4302536.4359007/94‑1 (MQ=60)

                                        AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      <  SRR4302536.4510938/94‑1 (MQ=60)

                                        AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      <  SRR4302536.2624891/94‑1 (MQ=60)

                                        AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      >  SRR4302536.1097596/1‑94 (MQ=60)

                                           ACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATC                                                   >  SRR4302536.2853253/1‑94 (MQ=60)

                                            CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302536.774401/94‑1 (MQ=60)

                                            CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302536.1619074/94‑1 (MQ=60)

                                            CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  >  SRR4302536.877262/1‑94 (MQ=60)

                                            CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302536.1006468/94‑1 (MQ=60)

                                             GATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCG                                                 >  SRR4302536.632881/1‑94 (MQ=60)

                                                CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              <  SRR4302536.5203453/94‑1 (MQ=60)

                                                CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              <  SRR4302536.6018791/94‑1 (MQ=60)

                                                 tgaagccgaCAATCCAGGACGATGGCTGCGAAAGCGGCACATGCAAGATCTAATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCT                                             <  SRR4302536.6284887/85‑1 (MQ=60)

                                                 TGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCT                                             <  SRR4302536.1726858/94‑1 (MQ=60)

                                                 TGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCT                                             <  SRR4302536.689829/94‑1 (MQ=60)

                                                  GGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTA                                            <  SRR4302536.2550102/94‑1 (MQ=60)

                                                    TGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACG                                          >  SRR4302536.2117759/1‑94 (MQ=60)

                                                     GCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGG                                         >  SRR4302536.3090435/1‑94 (MQ=60)

                                                     GCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGG                                         <  SRR4302536.3835164/94‑1 (MQ=60)

                                                      CCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGC                                        >  SRR4302536.4141360/1‑94 (MQ=60)

                                                      CCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGC                                        >  SRR4302536.3985173/1‑94 (MQ=60)

                                                       CGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCT                                       <  SRR4302536.2561317/94‑1 (MQ=60)

                                                        GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302536.3477640/1‑94 (MQ=60)

                                                        GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302536.4920219/1‑94 (MQ=60)

                                                        GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302536.4920218/1‑94 (MQ=60)

                                                        GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      <  SRR4302536.5843887/94‑1 (MQ=60)

                                                        GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      <  SRR4302536.5997120/94‑1 (MQ=60)

                                                        GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302536.6246044/1‑94 (MQ=60)

                                                         TCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCG                                     <  SRR4302536.4537950/94‑1 (MQ=60)

                                                         TCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCG                                     <  SRR4302536.1358726/94‑1 (MQ=60)

                                                          CAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG                                    <  SRR4302536.5651220/94‑1 (MQ=60)

                                                          CAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG                                    >  SRR4302536.1134010/1‑94 (MQ=60)

                                                          CAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG                                    <  SRR4302536.6360613/94‑1 (MQ=60)

                                                           AATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGT                                   >  SRR4302536.3377752/1‑94 (MQ=60)

                                                            ATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTT                                  <  SRR4302536.2542043/94‑1 (MQ=60)

                                                             TCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTT                                 <  SRR4302536.4502977/94‑1 (MQ=60)

                                                             TCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTT                                 <  SRR4302536.520691/94‑1 (MQ=60)

                                                             ACCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTT                                 <  SRR4302536.5615317/94‑1 (MQ=60)

                                                              CCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTG                                >  SRR4302536.3187335/1‑94 (MQ=60)

                                                              CCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTG                                >  SRR4302536.2603123/1‑94 (MQ=60)

                                                               CAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGT                               >  SRR4302536.5003519/1‑94 (MQ=60)

                                                                AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              >  SRR4302536.3740827/1‑94 (MQ=60)

                                                                 GGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAG                             <  SRR4302536.5006351/94‑1 (MQ=60)

                                                                  GACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGG                            >  SRR4302536.3283552/1‑94 (MQ=60)

                                                                  GACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGG                            >  SRR4302536.5795104/1‑94 (MQ=60)

                                                                    CGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTTACCGGCCTACGGCTCGGTTTGTAGGTC                          >  SRR4302536.5551771/1‑94 (MQ=60)

                                                                    CGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTTTAGGCC                          >  SRR4302536.2838231/1‑94 (MQ=60)

                                                                     GATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCT                         <  SRR4302536.6531515/94‑1 (MQ=60)

                                                                     GATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCT                         <  SRR4302536.4120078/94‑1 (MQ=60)

                                                                     GATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAAGCCT                         >  SRR4302536.3001005/1‑94 (MQ=60)

                                                                      ATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTG                        >  SRR4302536.1516471/1‑94 (MQ=60)

                                                                       TGGCTGCGAAGGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCC‑TATCCGGCCTACGGCTCGGTTTGTAGGCCTGAT                      <  SRR4302536.435707/94‑1 (MQ=60)

                                                                         GCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATCGGTTTGTAGGCCTGATA                     <  SRR4302536.2958102/94‑1 (MQ=60)

                                                                          CTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAA                    >  SRR4302536.4064994/1‑94 (MQ=60)

                                                                           TGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAG                   <  SRR4302536.4784082/94‑1 (MQ=60)

                                                                            GCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGA                  <  SRR4302536.1843741/94‑1 (MQ=60)

                                                                             CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCcggttgcatggctggtaaaac                 >  SRR4302536.748953/1‑73 (MQ=60)

                                                                             CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 >  SRR4302536.1319413/1‑94 (MQ=60)

                                                                             CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 <  SRR4302536.4573190/94‑1 (MQ=60)

                                                                             CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAACAC                 <  SRR4302536.6601857/94‑1 (MQ=60)

                                                                              GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                >  SRR4302536.2610764/1‑94 (MQ=60)

                                                                              GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                <  SRR4302536.145109/94‑1 (MQ=60)

                                                                              GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                <  SRR4302536.6206576/94‑1 (MQ=60)

                                                                              GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                >  SRR4302536.5514067/1‑94 (MQ=60)

                                                                              GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAAG                >  SRR4302536.4999284/1‑94 (MQ=60)

                                                                               AAATCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               >  SRR4302536.2515552/1‑94 (MQ=60)

                                                                               AAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               <  SRR4302536.4556648/94‑1 (MQ=60)

                                                                                AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGGCTGATAAGACGCG              >  SRR4302536.5001592/1‑94 (MQ=60)

                                                                                AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCG              <  SRR4302536.5307301/94‑1 (MQ=60)

                                                                                AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCG              >  SRR4302536.1128797/1‑94 (MQ=60)

                                                                                AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCG              <  SRR4302536.4467118/94‑1 (MQ=60)

                                                                                AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGACGCG              >  SRR4302536.1009877/1‑94 (MQ=60)

                                                                                 AGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGC             >  SRR4302536.2869655/1‑94 (MQ=60)

                                                                                   CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           <  SRR4302536.4676158/94‑1 (MQ=60)

                                                                                   CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           <  SRR4302536.3311652/94‑1 (MQ=60)

                                                                                   CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           >  SRR4302536.3953692/1‑94 (MQ=60)

                                                                                   CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAAGACTGATAAGAAGCGCCA           >  SRR4302536.2929119/1‑94 (MQ=60)

                                                                                    GTCGCATGCAAGATCTGATATTGAGATGCCGGAAGCAGCGTAAACTCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAG          <  SRR4302536.6478526/94‑1 (MQ=56)

                                                                                    GGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGACGCGCCAG          >  SRR4302536.5767690/1‑94 (MQ=60)

                                                                                     GCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGC         >  SRR4302536.2801080/1‑94 (MQ=60)

                                                                                     GCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGC         <  SRR4302536.5308561/94‑1 (MQ=60)

                                                                                      CGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCG        <  SRR4302536.3144348/94‑1 (MQ=60)

                                                                                      CGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCG        <  SRR4302536.6358862/94‑1 (MQ=60)

                                                                                      CGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTTATAAGACGCGCCAGCG        >  SRR4302536.554473/1‑94 (MQ=60)

                                                                                        CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      <  SRR4302536.5795652/94‑1 (MQ=60)

                                                                                        CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      >  SRR4302536.3109139/1‑94 (MQ=60)

                                                                                        CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAAGCGCCcaactc      >  SRR4302536.28004/1‑88 (MQ=60)

                                                                                        CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAAGCGCCCGCGCC      >  SRR4302536.1402605/1‑94 (MQ=60)

                                                                                         ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     >  SRR4302536.1134964/1‑94 (MQ=60)

                                                                                         ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     >  SRR4302536.6224264/1‑94 (MQ=60)

                                                                                         AGGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     <  SRR4302536.2279336/94‑1 (MQ=60)

                                                                                          TGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGC    >  SRR4302536.853846/1‑94 (MQ=60)

                                                                                           GCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCA   <  SRR4302536.5741244/94‑1 (MQ=60)

                                                                                           GCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCA   >  SRR4302536.1113143/1‑94 (MQ=60)

                                                                                           GCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCA   >  SRR4302536.5320550/1‑94 (MQ=60)

                                                                                            CAAGATCTGCTATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGGCTGATAAGACGCGCCAGCGTCGCAT  >  SRR4302536.6739518/1‑94 (MQ=60)

CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345069‑2345254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`