Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,360,682 T→C A324A (GCA→GCG)  b2249 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,360,6820TC100.0% 325.7 / NA 103A324A (GCA→GCG) b2249hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (62/41);  total (62/41)

Only 100 of 137 total aligned reads displayed.

AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGTGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACA  >  NC_000913/2360592‑2360771
                                                                                           |                                                                                          
aGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAgcgc                                                                                          >  2:1244473/1‑93 (MQ=255)
 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGcc                                                                                         >  1:158338/1‑93 (MQ=255)
 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGcc                                                                                         >  1:1406355/1‑93 (MQ=255)
  tGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCa                                                                                        <  2:4439737/93‑1 (MQ=255)
    cTGAAACGCACACGCAGAGCGAAAGTGCCGGCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAAAAgccagcgccagc                                                                                      >  1:102702/1‑93 (MQ=255)
     tGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGcc                                                                                     <  2:1864821/93‑1 (MQ=255)
     tGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGcc                                                                                     <  2:2724260/93‑1 (MQ=255)
       aaaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑g                                                                                  >  2:2538372/1‑93 (MQ=255)
        aaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑gg                                                                                 >  2:4090687/1‑93 (MQ=255)
        aaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑gg                                                                                 >  1:4032972/1‑93 (MQ=255)
         aCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGc                                                                                <  1:67747/93‑1 (MQ=255)
         aCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGc                                                                                >  2:633333/1‑93 (MQ=255)
           gCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCaa                                                                              >  1:3547512/1‑93 (MQ=255)
             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTATCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCaaaa                                                                            <  1:2907600/93‑1 (MQ=255)
             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCaaaa                                                                            <  1:4207310/93‑1 (MQ=255)
             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCaaaa                                                                            <  1:2099085/93‑1 (MQ=255)
                 gCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGt                                                                        <  2:2810858/93‑1 (MQ=255)
                 gCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGt                                                                        <  2:2810859/93‑1 (MQ=255)
                 gCAGAGCGAAAGTGCCGTCTGGCGTGGCTAACGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGt                                                                        <  2:838808/93‑1 (MQ=255)
                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGtt                                                                       <  2:809567/93‑1 (MQ=255)
                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGtt                                                                       <  2:1813442/93‑1 (MQ=255)
                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGtt                                                                       <  2:1155858/93‑1 (MQ=255)
                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGtt                                                                       <  1:2086074/93‑1 (MQ=255)
                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGtt                                                                       <  1:2623519/93‑1 (MQ=255)
                    gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTgg                                                                     >  1:2234781/1‑93 (MQ=255)
                     agCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGc                                                                    >  1:3049535/1‑93 (MQ=255)
                     agCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGc                                                                    >  1:68855/1‑93 (MQ=255)
                      gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGcc                                                                   >  1:4647821/1‑93 (MQ=255)
                      gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGcc                                                                   <  1:3202570/93‑1 (MQ=255)
                        gAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCccg                                                                 >  2:868472/1‑93 (MQ=255)
                         aaaGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGccccc                                                                >  2:1286918/1‑91 (MQ=255)
                         aaaGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCccgc                                                                >  2:3572161/1‑93 (MQ=255)
                           aGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCccgccg                                                              >  1:2440246/1‑93 (MQ=255)
                            gTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGt                                                             <  2:4340718/93‑1 (MQ=255)
                            gTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGt                                                             >  2:657890/1‑93 (MQ=255)
                               ccGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCt                                                          >  1:4348761/1‑93 (MQ=255)
                               ccGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCt                                                          >  1:4604086/1‑93 (MQ=255)
                                cGTCTGGCGTGGCTAGCGCAAAGTTGAGATCCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTg                                                         <  2:131249/93‑1 (MQ=255)
                                cGTCGGGCGTGGTTGCCGAAATGTTGAGATTCT‑GTTCTTAAAACCCGGAAACAGCCGGCGCCAGCCC‑GGAAAATTGGTTGGCCCGCCGTTCTg                                                         <  2:3409283/93‑1 (MQ=255)
                                   cgggCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTaa                                                      <  1:3285288/91‑1 (MQ=255)
                                   cTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTaa                                                      <  1:1425020/93‑1 (MQ=255)
                                    tGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAAt                                                     >  2:1920786/1‑93 (MQ=255)
                                    tGGCGTGGCTAGCGAAAAGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAAt                                                     <  2:229554/93‑1 (MQ=255)
                                      gCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATcc                                                   >  1:51412/1‑93 (MQ=255)
                                       cGTGGCTAGCGCAAAGTTGAGAAGCTCGTTCTCGAACCCG‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCa                                                  <  1:4205998/93‑1 (MQ=255)
                                        gTGGCTAGCGAAAAGTTGAGATTCTCGTCCCCGAACCCC‑AAAACACCCAGCGCCGGCCC‑GGCAAAATGTTGGCCCCGCCGTTCTGTAATCCAg                                                 <  2:4140704/93‑1 (MQ=255)
                                         tGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGt                                                <  2:2734079/93‑1 (MQ=255)
                                         tGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGt                                                >  2:72396/1‑93 (MQ=255)
                                         tGGCTAGCGCAAAGTTGAGATCCTCGTTCTCAAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGt                                                <  2:2940028/93‑1 (MQ=255)
                                          ggCTAGCGCAAAGTTGAGATCCTCGTTCTCGAACCCG‑GAAACAGCCAGCGCCAGCCC‑GGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTg                                               <  1:3073194/93‑1 (MQ=255)
                                             tAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATGCAGTgcgc                                            >  2:623900/1‑93 (MQ=255)
                                              aGCGCAAAGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCa                                           <  1:104002/93‑1 (MQ=255)
                                              aGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCACAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCa                                           >  2:1705391/1‑93 (MQ=255)
                                               gcgcAAGGTTGAGATGCTCGTTCGCGAACCCG‑GAACCAGCCAGCGACAGCCC‑GGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAg                                          <  1:4476979/93‑1 (MQ=255)
                                               gcgcAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAg                                          >  2:4297589/1‑93 (MQ=255)
                                                 gcAAAGTTGAGATGCTCGTCCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGtt                                        <  2:90836/93‑1 (MQ=255)
                                                 gaaaaGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGtt                                        <  2:3239037/91‑1 (MQ=255)
                                                    aaGTTGAGATGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTgc                                     <  1:3797904/93‑1 (MQ=255)
                                                     aGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTgcg                                    >  1:1515812/1‑93 (MQ=255)
                                                      gTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTgcgc                                   >  1:662069/1‑93 (MQ=255)
                                                        tGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCa                                 >  2:2805381/1‑93 (MQ=255)
                                                           gaTGCTCGTTCTCGAACCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAggg                              <  1:4443286/93‑1 (MQ=255)
                                                           gaTGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAggg                              >  1:2150529/1‑93 (MQ=255)
                                                            aTGCTCATCCTCGACACCC‑GAACCCGCCCG‑GCCTTCCCAGGTAAAATGGTTGACCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCTAGGGt                             <  2:2329354/93‑1 (MQ=255)
                                                             tGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGtt                            >  1:3859582/1‑93 (MQ=255)
                                                             tGCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGCTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGtt                            <  2:3910261/93‑1 (MQ=255)
                                                              gCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGttt                           >  2:4120912/1‑93 (MQ=255)
                                                              gCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGttt                           >  2:4120911/1‑93 (MQ=255)
                                                              gCTCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGttt                           <  2:4076787/93‑1 (MQ=255)
                                                                tCGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcc                         >  1:1600963/1‑93 (MQ=255)
                                                                ccGTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcc                         <  1:3257563/92‑1 (MQ=255)
                                                                  gTTCTCGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcctc                       >  2:2178407/1‑93 (MQ=255)
                                                                   ttCTCGAACCCC‑GAAACAGCCAGCGCCAGCCG‑GGCAAAATGTTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcctcc                      <  1:1069382/93‑1 (MQ=255)
                                                                    tctcGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCAGCCGTTCTGTAATCCAGTGAGCAGTTTGCGCCAGGGTTTcctcct                     >  1:2215928/1‑93 (MQ=255)
                                                                     ctcAAACCCC‑GAAACAGCCAGCGCCAGCCG‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtg                    <  1:2904785/93‑1 (MQ=255)
                                                                     ctcAAAACCA‑GAAACAGCCAGCCCCAGCCA‑GAAAAAAGGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtg                    <  1:3392958/93‑1 (MQ=255)
                                                                      tcGAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtgt                   >  2:92924/1‑93 (MQ=255)
                                                                      cggAACCCC‑GAAACCGCACGCGCAAGCCC‑GCCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtgt                   <  2:1223881/91‑1 (MQ=255)
                                                                        gAAACCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGa                 >  1:4562831/1‑93 (MQ=255)
                                                                         agcccc‑AAAACAGACAGCGCCACCCC‑GGCAAAGTGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGaa                <  1:2274910/90‑1 (MQ=255)
                                                                         aaaCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGaa                <  1:3802065/93‑1 (MQ=255)
                                                                          aaCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAg               >  2:1426997/1‑93 (MQ=255)
                                                                           aCCC‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAgg              >  1:634987/1‑93 (MQ=255)
                                                                            ccc‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGa             >  2:328064/1‑93 (MQ=255)
                                                                            ccc‑GAAACAGCCAGCCCCACCCC‑GGCAAATGTTTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGa             <  1:1749333/93‑1 (MQ=255)
                                                                              c‑GAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc           >  2:290604/1‑93 (MQ=255)
                                                                                gAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAcc          >  1:3596935/1‑93 (MQ=255)
                                                                                gAAACAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAcc          >  2:1008992/1‑93 (MQ=255)
                                                                                aaaaCAGCCAGCGCCAGCCC‑GGAAAAAGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAcc          <  1:4147773/92‑1 (MQ=255)
                                                                                 aaaCAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCa         >  1:1634054/1‑93 (MQ=255)
                                                                                 aaaCAGCCAGCGCCAGCCC‑GGAAAAATGGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCa         <  2:1437952/93‑1 (MQ=255)
                                                                                 aaaCAGCCAGCCCCGGCCC‑GGAAAAATGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCa         <  2:34116/93‑1 (MQ=255)
                                                                                  aaCAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCAc        >  2:3077391/1‑93 (MQ=255)
                                                                                   aCAGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACt       >  2:4046517/1‑93 (MQ=255)
                                                                                   aCAGCCAGCCCCAGCCC‑GGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACt       <  2:720505/93‑1 (MQ=255)
                                                                                    cagccagcGCCCGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACtt      <  1:3986316/93‑1 (MQ=255)
                                                                                     aGCCAGCGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTc     >  1:2736696/1‑93 (MQ=255)
                                                                                       ccAGCGCCCGCCG‑GACAAAAGGGTGGTCCCGCCGTTCTTTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTcac   <  2:3837062/93‑1 (MQ=255)
                                                                                       ccAGCGCCACCCC‑GGCAAAATGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTcac   <  2:1606182/93‑1 (MQ=255)
                                                                                        cAGCGCCAGCCC‑GGCAAAATGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTcaca  <  1:1289319/93‑1 (MQ=255)
                                                                                           |                                                                                          
AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCC‑GAAACAGCCAGTGCCAGCCC‑GGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACA  >  NC_000913/2360592‑2360771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC  >  NC_000913/2360589‑2360775
                                                                                             |                                                                                             
CGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGC                                                                                               >  SRR4302544.1860639/1‑94 (MQ=60)
   AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCC                                                                                            >  SRR4302544.1420359/1‑94 (MQ=60)
   AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCC                                                                                            >  SRR4302544.159886/1‑94 (MQ=60)
    GTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCTAAACCCGAAACAGCCAGCGCCA                                                                                           >  SRR4302544.1488508/1‑94 (MQ=60)
      GCTGAAACGCACACGCAGAGCGAAAGTGCCGGCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAAAAGCCAGCGCCAGC                                                                                         >  SRR4302544.103642/1‑94 (MQ=60)
         GAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCG                                                                                      >  SRR4302544.2432171/1‑94 (MQ=60)
         GAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCG                                                                                      <  SRR4302544.4571678/94‑1 (MQ=60)
          AAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGG                                                                                     >  SRR4302544.4073145/1‑94 (MQ=60)
           AACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGC                                                                                    >  SRR4302544.2229376/1‑94 (MQ=60)
            ACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCA                                                                                   <  SRR4302544.68419/94‑1 (MQ=60)
             CGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAA                                                                                  >  SRR4302544.3581486/1‑94 (MQ=60)
               CACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAA                                                                                >  SRR4302544.1295270/1‑94 (MQ=60)
                ACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTATCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAAT                                                                               <  SRR4302544.2935581/94‑1 (MQ=60)
                ACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAAT                                                                               <  SRR4302544.4249555/94‑1 (MQ=60)
                ACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAAT                                                                               <  SRR4302544.2119284/94‑1 (MQ=60)
                     CAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTG                                                                          <  SRR4302544.2648676/94‑1 (MQ=60)
                     CAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTG                                                                          <  SRR4302544.2106142/94‑1 (MQ=60)
                      AGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGG                                                                         >  SRR4302544.2255800/1‑94 (MQ=60)
                       GAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGC                                                                        >  SRR4302544.3078677/1‑94 (MQ=60)
                       GAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGC                                                                        >  SRR4302544.69533/1‑94 (MQ=60)
                        AGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCC                                                                       >  SRR4302544.4693834/1‑94 (MQ=60)
                         GCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCC                                                                      <  SRR4302544.3233092/94‑1 (MQ=60)
                             AAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCG                                                                  <  SRR4302544.2978340/94‑1 (MQ=60)
                             AAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCG                                                                  >  SRR4302544.2463513/1‑94 (MQ=60)
                              AGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGT                                                                 <  SRR4302544.695445/94‑1 (MQ=60)
                               GTGCCGTCTGGCGTGGCTAGCGCAAATTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTT                                                                <  SRR4302544.3553572/94‑1 (MQ=60)
                                 GCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCT                                                              >  SRR4302544.4392958/1‑94 (MQ=60)
                                 GCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCT                                                              >  SRR4302544.4392957/1‑94 (MQ=60)
                                 GCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCT                                                              >  SRR4302544.4649844/1‑94 (MQ=60)
                                    GTCTGGCGTGGCTAGCCCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACACCCAGCCCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTA                                                           <  SRR4302544.567094/94‑1 (MQ=60)
                                    GTCGGGCTTGGCTAGCGCAAAGTTGAGTTGCTCGTCCCCGAACCCCGAAACAGCCGGCCCCAGCCGGGCAAAATGTTTGGCCCGCCGTTCTGTA                                                           <  SRR4302544.1519351/94‑1 (MQ=36)
                                      CTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAAT                                                         <  SRR4302544.1439200/94‑1 (MQ=60)
                                      CGGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAAT                                                         <  SRR4302544.3316596/94‑1 (MQ=60)
                                        GGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCC                                                       >  SRR4302544.51935/1‑94 (MQ=60)
                                          CGTGGCTAGCGCAAAGTTGAGAAGCTCGTTCTCGAACCCGGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAG                                                     <  SRR4302544.4248191/94‑1 (MQ=60)
                                             GGCTAGCGCAAAGTTGAGATGCTCTTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGC                                                  <  SRR4302544.3287002/94‑1 (MQ=60)
                                             GGCTAGCGCAAAGTTGAGATCCTCGTTCTCGAACCCGGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGC                                                  <  SRR4302544.3102485/94‑1 (MQ=60)
                                               CTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGC                                                <  SRR4302544.2759257/94‑1 (MQ=60)
                                                 AGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAG                                              <  SRR4302544.104958/94‑1 (MQ=60)
                                                  GCGCAAGGTTGAGATGCTCGTTCGCGAACCCGGAACCAGCCAGCGACAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGT                                             <  SRR4302544.4521994/94‑1 (MQ=60)
                                                   ggtAAAGTTGAGATGCGCTTTCTCGAAACCCAAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTT                                            <  SRR4302544.3726623/91‑1 (MQ=60)
                                                    GAAAATTTGAAATGCTTGTTCTCAAACCCCGAAACAGCCACCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTT                                           <  SRR4302544.1443859/94‑1 (MQ=60)
                                                       AAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCG                                        <  SRR4302544.3835517/94‑1 (MQ=60)
                                                       AAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCG                                        >  SRR4302544.1530845/1‑94 (MQ=60)
                                                        AGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGC                                       >  SRR4302544.668612/1‑94 (MQ=60)
                                                             AGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGG                                  >  SRR4302544.2170997/1‑94 (MQ=60)
                                                              GATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGT                                 <  SRR4302544.4488074/94‑1 (MQ=60)
                                                               ATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTT                                >  SRR4302544.3897900/1‑94 (MQ=60)
                                                                  CTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCC                             >  SRR4302544.1648570/1‑94 (MQ=60)
                                                                  CTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCC                             >  SRR4302544.1616752/1‑94 (MQ=60)
                                                                   CCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCT                            <  SRR4302544.3288699/94‑1 (MQ=60)
                                                                      TTCTCGAACCCCGAAACAGCCAGCGCCAGCCGGGCAAAATGTTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCT                         <  SRR4302544.1080009/94‑1 (MQ=60)
                                                                      TTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCT                         <  SRR4302544.653000/94‑1 (MQ=60)
                                                                      TTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCAGCCGTTCTGTAATCCAGTGAGCAGTTTGCGCCAGGGTTTCCTCCT                         >  SRR4302544.2236772/1‑94 (MQ=60)
                                                                        CTCAAACCCCGAAACAGCCAGCGCCAGCCGGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGT                       <  SRR4302544.2932753/94‑1 (MQ=60)
                                                                         ccgaaccccaAAACAGCCAGCCCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTG                      <  SRR4302544.1812027/84‑1 (MQ=60)
                                                                          CGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGA                     >  SRR4302544.4608327/1‑94 (MQ=60)
                                                                           GAAACCCGAAACAGCCAGCGCCAGCCCGGCATAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAA                    >  SRR4302544.493079/1‑94 (MQ=60)
                                                                            agccccaAAACAGACAGCGCCACCCCGGCAAAGTGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAG                   <  SRR4302544.2296325/87‑1 (MQ=60)
                                                                            aacccaaAAACAGCCAGCGCCAGCCGGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAG                   <  SRR4302544.1024866/87‑1 (MQ=60)
                                                                            AAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGTCATGGTTTCCTCCTGTGATG                   >  SRR4302544.4247612/1‑94 (MQ=60)
                                                                            AAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAG                   <  SRR4302544.3839733/94‑1 (MQ=60)
                                                                             AACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGG                  >  SRR4302544.641248/1‑94 (MQ=60)
                                                                               CCCGAAACAGCCAGCCCCACCCCGGCAAATGTTTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAA                <  SRR4302544.1766458/94‑1 (MQ=60)
                                                                                cgaAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAC               <  SRR4302544.3928137/91‑1 (MQ=60)
                                                                                 CGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACC              >  SRR4302544.3436150/1‑94 (MQ=60)
                                                                                 CGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACC              >  SRR4302544.3631565/1‑94 (MQ=60)
                                                                                  GAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCA             >  SRR4302544.1650132/1‑94 (MQ=60)
                                                                                  AAAACAGCCAGCGCCAGCCCGGAAAAAGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCA             <  SRR4302544.4188842/94‑1 (MQ=60)
                                                                                      CAGCCAGCGCCCGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTC         <  SRR4302544.4026000/94‑1 (MQ=60)
                                                                                      CAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTC         >  SRR4302544.2763076/1‑94 (MQ=60)
                                                                                          CAGCGCCAGCCCGGCAAAATGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACAC     <  SRR4302544.1302016/94‑1 (MQ=60)
                                                                                             CGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC  >  SRR4302544.1644076/1‑94 (MQ=60)
                                                                                             CGCCAGCCCGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC  <  SRR4302544.4404216/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC  >  NC_000913/2360589‑2360775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑