Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,362,769 G→A E66K (GAA→AAA)  yfaO → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,362,7690GA99.5% 713.3 / ‑5.3 201E66K (GAA→AAA) yfaOhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (117/83);  total (118/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 209 total aligned reads displayed.

CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362680‑2362858
                                                                                         |                                                                                         
cGGGAGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGCACTGGAAGAACAGCTCCTTTTGACAaaaa                                                                                        <  2:924596/93‑1 (MQ=255)
 gggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAt                                                                                       >  1:3930897/1‑93 (MQ=255)
 gggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAt                                                                                       >  1:3193005/1‑93 (MQ=255)
 gggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAt                                                                                       <  2:4632591/93‑1 (MQ=255)
 gggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAt                                                                                       >  1:3378584/1‑93 (MQ=255)
 gggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAt                                                                                       <  2:2140/93‑1 (MQ=255)
    tGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCAc                                                                                    <  2:2380289/93‑1 (MQ=255)
     ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   <  1:1044082/93‑1 (MQ=255)
      gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  1:1277592/93‑1 (MQ=255)
       cggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAAAAGCTGCTTTTGACAAAAATCACGcc                                                                                 >  2:2185068/1‑93 (MQ=255)
        ggcgTGGAGCGTGGCGAACGAATTGAAGAGTCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCg                                                                                <  2:1762745/93‑1 (MQ=255)
        ggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCg                                                                                >  2:297781/1‑93 (MQ=255)
        ggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCg                                                                                <  1:2876600/93‑1 (MQ=255)
         gcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGt                                                                               >  1:3438836/1‑93 (MQ=255)
           gTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTgg                                                                             >  2:4276470/1‑93 (MQ=255)
            tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            <  2:3333466/93‑1 (MQ=255)
            tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            >  1:646228/1‑93 (MQ=255)
               aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         >  1:3517736/1‑93 (MQ=255)
               aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         >  1:2439552/1‑93 (MQ=255)
                gCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCtt                                                                        >  2:1930589/1‑93 (MQ=255)
                  cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCa                                                                      <  1:56088/93‑1 (MQ=255)
                  cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCa                                                                      <  1:3228514/93‑1 (MQ=255)
                  cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCa                                                                      >  2:2887019/1‑93 (MQ=255)
                    ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    <  1:3039829/93‑1 (MQ=255)
                    ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    >  2:1257149/1‑93 (MQ=255)
                     gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCg                                                                   >  1:4138112/1‑93 (MQ=255)
                     gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   >  1:90349/1‑93 (MQ=255)
                     gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   >  2:2687243/1‑93 (MQ=255)
                      cgaacggaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCga                                                                  >  1:3163036/1‑93 (MQ=255)
                        aacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatg                                                                >  1:388647/1‑93 (MQ=255)
                          cgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatgat                                                              >  2:4376903/1‑93 (MQ=255)
                           gaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGTTGata                                                             >  2:1629968/1‑93 (MQ=255)
                           gaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGata                                                             >  1:750454/1‑93 (MQ=255)
                            aaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGGGGACCTTCAGCGATGatat                                                            >  1:4615807/1‑93 (MQ=255)
                             aTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAtt                                                           <  2:4680514/93‑1 (MQ=255)
                              ttGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTc                                                          >  2:2938889/1‑93 (MQ=255)
                                     ggCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCaa                                                   >  1:364786/1‑93 (MQ=255)
                                       cACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGa                                                 <  1:1379606/93‑1 (MQ=255)
                                       cACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGa                                                 >  2:3637823/1‑93 (MQ=255)
                                       cACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGa                                                 <  1:2034830/93‑1 (MQ=255)
                                       cACTACGCAGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCCCGACGTGGACCTTCAGCGATGATATTCGCACCAAGa                                                 <  1:1201437/93‑1 (MQ=255)
                                        aCTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAc                                                <  1:3799966/93‑1 (MQ=255)
                                          tACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGt                                              >  1:3784089/1‑93 (MQ=255)
                                          tACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGt                                              >  2:3079465/1‑93 (MQ=255)
                                           aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             <  2:2542622/93‑1 (MQ=255)
                                           aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             >  2:2744036/1‑93 (MQ=255)
                                            cgccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAt                                            <  1:1250065/93‑1 (MQ=255)
                                            cgccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAt                                            <  2:1145025/93‑1 (MQ=255)
                                             gccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATg                                           >  2:2242878/1‑93 (MQ=255)
                                             gccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATCCGCACCAAGACGTATg                                           >  2:1565948/1‑93 (MQ=255)
                                              ccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGc                                          >  2:4230796/1‑93 (MQ=255)
                                                 cgAAATTCGCGAAGAACTGGGAGAACAGTGACTGTGGACAAAGATTACGCCGTGGACTGTCAGCGATGATATTCGATTCAGGATGGATGCAGa                                       <  2:2717647/93‑1 (MQ=255)
                                                 cgAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGa                                       <  1:1589767/93‑1 (MQ=255)
                                                  gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCTCACCAAGACGTATGCAGAt                                      >  2:605989/1‑93 (MQ=255)
                                                  gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  1:2793765/93‑1 (MQ=255)
                                                  gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  2:3885029/93‑1 (MQ=255)
                                                  gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  2:4719756/93‑1 (MQ=255)
                                                  gAAATTCGAGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      >  1:488579/1‑93 (MQ=255)
                                                  gAAAATGGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  1:2134938/93‑1 (MQ=255)
                                                   aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     >  2:1215626/1‑93 (MQ=255)
                                                    aaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATgg                                    >  2:4615647/1‑93 (MQ=255)
                                                     aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   >  1:4542641/1‑93 (MQ=255)
                                                      ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  <  2:475193/93‑1 (MQ=255)
                                                       tCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCg                                 >  1:2674054/1‑93 (MQ=255)
                                                          cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              >  1:2715821/1‑93 (MQ=255)
                                                          cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              >  1:2808630/1‑93 (MQ=255)
                                                           gaagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATCTTCGCACCAAGACGTATGCAGATGGTCGCAAg                             >  2:1644407/1‑93 (MQ=255)
                                                           gaagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAg                             <  1:3391266/93‑1 (MQ=255)
                                                            aagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAgg                            >  2:3493814/1‑93 (MQ=255)
                                                            aagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAgg                            >  1:560009/1‑93 (MQ=255)
                                                             agaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGa                           >  2:3422492/1‑93 (MQ=255)
                                                              gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGaa                          >  2:1284740/1‑93 (MQ=255)
                                                              gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGaa                          >  1:2407863/1‑93 (MQ=255)
                                                              gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGaa                          >  2:1783940/1‑93 (MQ=255)
                                                               aaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAag                         >  1:398798/1‑93 (MQ=255)
                                                                aCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAaga                        <  1:2395607/93‑1 (MQ=255)
                                                                  tGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAgaga                      >  2:1929484/1‑93 (MQ=255)
                                                                   gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     >  2:1464017/1‑93 (MQ=255)
                                                                   gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     >  2:3595965/1‑93 (MQ=255)
                                                                   gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     >  1:3916317/1‑93 (MQ=255)
                                                                      agaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTta                  <  2:950049/93‑1 (MQ=255)
                                                                       gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  1:2957595/1‑93 (MQ=255)
                                                                        aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                <  2:2830695/93‑1 (MQ=255)
                                                                        aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                >  1:1644677/1‑93 (MQ=255)
                                                                        aaCAGCTGCTTTTGACAAAAATCACACCGTGGACCTTCAGCGATGATATTCGCACCCAGACGTATGCAGATGGTCGCCAGGAAGAGATTtaga                >  2:53490/1‑91 (MQ=255)
                                                                        aaCAGCTGCTTTTGACAAAAATCACACCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                >  2:53492/1‑93 (MQ=255)
                                                                         aCAGCTGCTTTTGACAAAAATCATGCCGTGGACCTTTAGCGATGATATTCGCACCATGACGTCTGCAGAAGGACGCTAGGAAGAGACatatat               >  2:2352895/1‑93 (MQ=255)
                                                                         aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               >  1:4121205/1‑93 (MQ=255)
                                                                         aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               >  2:768013/1‑93 (MQ=255)
                                                                           aGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGa             <  1:1903303/93‑1 (MQ=255)
                                                                            gctgcgTTTGACAAAAATCACGCCGTGGACCTGCAGCGATGATATTCGCACCAAGACGTATGCAGATGGGCGCAAGGAAGAGATTTATATGAt            >  1:4203968/1‑93 (MQ=255)
                                                                             ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           <  1:2296341/93‑1 (MQ=255)
                                                                              tgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCGAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAttt          >  1:1766394/1‑93 (MQ=255)
                                                                              tgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAttt          <  1:3262088/93‑1 (MQ=255)
                                                                                 ttttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAcc       <  2:4183479/93‑1 (MQ=255)
                                                                                  tgtGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      <  2:3343168/91‑1 (MQ=255)
                                                                                   ttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTg     <  1:804469/93‑1 (MQ=255)
                                                                                     gACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAt   <  1:117570/93‑1 (MQ=255)
                                                                                     gACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAt   >  1:4400971/1‑93 (MQ=255)
                                                                                      aCAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAtt  >  2:3061045/1‑93 (MQ=255)
                                                                                         |                                                                                         
CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362680‑2362858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  NC_000913/2362676‑2362862
                                                                                             |                                                                                             
ATTTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAA                                                                                               >  SRR4302544.4442866/1‑94 (MQ=60)
    CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAT                                                                                           <  SRR4302544.1190998/94‑1 (MQ=60)
    CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAT                                                                                           >  SRR4302544.3969940/1‑94 (MQ=60)
    CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAT                                                                                           >  SRR4302544.3410835/1‑94 (MQ=60)
    CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAT                                                                                           >  SRR4302544.3410834/1‑94 (MQ=60)
    CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAT                                                                                           >  SRR4302544.3223435/1‑94 (MQ=60)
      GGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCA                                                                                         >  SRR4302544.717125/1‑94 (MQ=60)
         GGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGC                                                                                      <  SRR4302544.1054441/94‑1 (MQ=60)
          GCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302544.1290173/94‑1 (MQ=60)
          GCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACCGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302544.617840/94‑1 (MQ=60)
          GCGGCGTGGAGCATGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302544.1240396/94‑1 (MQ=60)
            GGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGT                                                                                   <  SRR4302544.2904363/94‑1 (MQ=60)
            GGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGT                                                                                   >  SRR4302544.3471711/1‑94 (MQ=60)
               GTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGA                                                                                >  SRR4302544.652609/1‑94 (MQ=60)
                  GAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCT                                                                             >  SRR4302544.4481948/1‑94 (MQ=60)
                  GAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCT                                                                             >  SRR4302544.3551328/1‑94 (MQ=60)
                  GAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCT                                                                             >  SRR4302544.2462808/1‑94 (MQ=60)
                     CCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCA                                                                          <  SRR4302544.758662/94‑1 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         <  SRR4302544.3259398/94‑1 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         <  SRR4302544.56653/94‑1 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         <  SRR4302544.3259397/94‑1 (MQ=60)
                       TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        >  SRR4302544.2332296/1‑94 (MQ=60)
                       TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        <  SRR4302544.24213/94‑1 (MQ=60)
                        GGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCG                                                                       >  SRR4302544.4179094/1‑94 (MQ=60)
                        GGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCG                                                                       <  SRR4302544.3068922/94‑1 (MQ=60)
                        GGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCG                                                                       >  SRR4302544.91200/1‑94 (MQ=60)
                         GCGAACGGATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGA                                                                      >  SRR4302544.3193125/1‑94 (MQ=60)
                         GCGAACGGATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGA                                                                      >  SRR4302544.3193124/1‑94 (MQ=60)
                          CGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGAT                                                                     <  SRR4302544.4169966/94‑1 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    >  SRR4302544.392595/1‑94 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    <  SRR4302544.2730954/94‑1 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    >  SRR4302544.1266629/1‑94 (MQ=60)
                            AACGAATTTAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGA                                                                   >  SRR4302544.2099485/1‑94 (MQ=60)
                            AACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGA                                                                   >  SRR4302544.2756656/1‑94 (MQ=60)
                              CGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATA                                                                 >  SRR4302544.757787/1‑94 (MQ=60)
                               GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                <  SRR4302544.3838185/94‑1 (MQ=60)
                               GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                >  SRR4302544.4425555/1‑94 (MQ=60)
                               GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGGGGACCTTCAGCGATGATAT                                                                >  SRR4302544.4661631/1‑94 (MQ=60)
                                AATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATT                                                               >  SRR4302544.2874912/1‑94 (MQ=60)
                                 ATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTC                                                              >  SRR4302544.3332943/1‑94 (MQ=60)
                                 ATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTC                                                              >  SRR4302544.3116563/1‑94 (MQ=60)
                                   TGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGC                                                            >  SRR4302544.3910094/1‑94 (MQ=60)
                                      AGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACC                                                         <  SRR4302544.2234588/94‑1 (MQ=60)
                                       GAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCA                                                        >  SRR4302544.4000928/1‑94 (MQ=60)
                                        AGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAA                                                       >  SRR4302544.368515/1‑94 (MQ=60)
                                           CACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAC                                                    <  SRR4302544.3011716/94‑1 (MQ=60)
                                           CACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAC                                                    <  SRR4302544.1393381/94‑1 (MQ=60)
                                           CACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAC                                                    <  SRR4302544.2054501/94‑1 (MQ=60)
                                           CACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAC                                                    <  SRR4302544.4597858/94‑1 (MQ=60)
                                           CACTACGCAGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCCCGACGTGGACCTTCAGCGATGATATTCGCACCAAGAC                                                    <  SRR4302544.1213339/94‑1 (MQ=60)
                                            ACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACG                                                   <  SRR4302544.3837604/94‑1 (MQ=60)
                                             CTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGT                                                  >  SRR4302544.2825901/1‑94 (MQ=60)
                                             CTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGT                                                  >  SRR4302544.3821558/1‑94 (MQ=60)
                                              TACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAGCTTCAGCGATGATATTCGCACCAAGACGTA                                                 >  SRR4302544.1394001/1‑94 (MQ=60)
                                               tcgcatctAAATTCTCGAAGAACTGGGAGAACATCTGCTTTTGACAAATATCACTCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAT                                                <  SRR4302544.4622128/86‑1 (MQ=60)
                                                CGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATG                                               <  SRR4302544.1262367/94‑1 (MQ=60)
                                                CGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCCGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATG                                               <  SRR4302544.2190521/94‑1 (MQ=60)
                                                   CGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAG                                            >  SRR4302544.2964926/1‑94 (MQ=60)
                                                   CGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAG                                            >  SRR4302544.2446730/1‑94 (MQ=60)
                                                     CGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          <  SRR4302544.1605449/94‑1 (MQ=60)
                                                     CGAAATTCGAGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          >  SRR4302544.493368/1‑94 (MQ=60)
                                                      GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         >  SRR4302544.4086096/1‑94 (MQ=60)
                                                      GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         <  SRR4302544.828785/94‑1 (MQ=60)
                                                      GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         <  SRR4302544.2820665/94‑1 (MQ=60)
                                                      GAAAATGGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         <  SRR4302544.2155355/94‑1 (MQ=60)
                                                       agaggcgagaaggactagaAGAACAGCTGTTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGG                                        <  SRR4302544.4235328/75‑1 (MQ=60)
                                                       AAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGG                                        <  SRR4302544.1882083/94‑1 (MQ=60)
                                                        AATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGT                                       >  SRR4302544.4588031/1‑94 (MQ=60)
                                                         ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      <  SRR4302544.2549327/94‑1 (MQ=60)
                                                          TTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCG                                     >  SRR4302544.2699689/1‑94 (MQ=60)
                                                             GCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAA                                  >  SRR4302544.2835720/1‑94 (MQ=60)
                                                             GCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAA                                  >  SRR4302544.2741961/1‑94 (MQ=60)
                                                               GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                <  SRR4302544.3423684/94‑1 (MQ=60)
                                                               GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                <  SRR4302544.3755703/94‑1 (MQ=60)
                                                               GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                >  SRR4302544.565481/1‑94 (MQ=60)
                                                               GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                >  SRR4302544.565482/1‑94 (MQ=60)
                                                               GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                >  SRR4302544.1939697/1‑94 (MQ=60)
                                                                 AGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAA                              >  SRR4302544.2430735/1‑94 (MQ=60)
                                                                 AGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAA                              >  SRR4302544.3441630/1‑94 (MQ=60)
                                                                  GAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAG                             <  SRR4302544.383803/94‑1 (MQ=60)
                                                                  GAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAG                             <  SRR4302544.394094/94‑1 (MQ=60)
                                                                  GAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAG                             >  SRR4302544.402839/1‑94 (MQ=60)
                                                                  GAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAG                             <  SRR4302544.3119310/94‑1 (MQ=60)
                                                                    ACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAG                           >  SRR4302544.3247210/1‑94 (MQ=60)
                                                                    ACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAG                           <  SRR4302544.2418319/94‑1 (MQ=60)
                                                                     CTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGA                          <  SRR4302544.520504/94‑1 (MQ=60)
                                                                      TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         >  SRR4302544.3955217/1‑94 (MQ=60)
                                                                      TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         <  SRR4302544.1610917/94‑1 (MQ=60)
                                                                      TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         >  SRR4302544.3955216/1‑94 (MQ=60)
                                                                         GAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTA                      >  SRR4302544.1307542/1‑94 (MQ=60)
                                                                          AGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTAT                     >  SRR4302544.2985994/1‑94 (MQ=60)
                                                                           GAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATA                    >  SRR4302544.1660891/1‑94 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   >  SRR4302544.1750475/1‑94 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   >  SRR4302544.4780278/1‑94 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   >  SRR4302544.4162110/1‑94 (MQ=60)
                                                                               AGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAT                <  SRR4302544.1921701/94‑1 (MQ=60)
                                                                               AGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAT                <  SRR4302544.362248/94‑1 (MQ=60)
                                                                               AGCTGCGTTTGACAAAAATCACGCCGTGGACCTGCAGCGATGATATTCGCACCAAGACGTATGCAGATGGGCGCAAGGAAGAGATTTATATGAT                >  SRR4302544.4246073/1‑94 (MQ=60)
                                                                                 gttCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTT              <  SRR4302544.4224447/91‑1 (MQ=60)
                                                                                 CTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCGAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTT              >  SRR4302544.1783680/1‑94 (MQ=60)
                                                                                 CTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTT              <  SRR4302544.2317996/94‑1 (MQ=60)
                                                                                 CTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTT              >  SRR4302544.543002/1‑94 (MQ=60)
                                                                                 CTGCTTTTGACAAAAATCACGCCGGGGACCTTCAGCGATGATATTCGCTCCAATGCGTATGCAGATagccggacggaagaagtttatctacttt              >  SRR4302544.1783681/1‑66 (MQ=50)
                                                                                  TGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTA             <  SRR4302544.3293242/94‑1 (MQ=60)
                                                                                   GCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAC            <  SRR4302544.1146883/94‑1 (MQ=60)
                                                                                    CTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACC           >  SRR4302544.3494524/1‑94 (MQ=60)
                                                                                       TTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGA        <  SRR4302544.812307/94‑1 (MQ=60)
                                                                                        TGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       >  SRR4302544.4445444/1‑94 (MQ=60)
                                                                                         GACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT      <  SRR4302544.118638/94‑1 (MQ=60)
                                                                                           CAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTT    >  SRR4302544.1639212/1‑94 (MQ=60)
                                                                                            AAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   <  SRR4302544.948880/94‑1 (MQ=60)
                                                                                            AAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   <  SRR4302544.971252/94‑1 (MQ=60)
                                                                                             AAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  <  SRR4302544.3087260/94‑1 (MQ=60)
                                                                                             AAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  SRR4302544.1725598/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  NC_000913/2362676‑2362862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑