Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,362,769 G→A E66K (GAA→AAA)  yfaO → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,362,7690GA99.6% 911.5 / ‑5.3 244E66K (GAA→AAA) yfaOhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (117/126);  total (117/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 251 total aligned reads displayed.

TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858
                                                                                          |                                                                                         
    gTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCa                                                                                     <  1:6037344/93‑1 (MQ=255)
     tGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCAc                                                                                    <  1:1971882/93‑1 (MQ=255)
       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:665898/93‑1 (MQ=255)
       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  1:466138/93‑1 (MQ=255)
       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:2663571/93‑1 (MQ=255)
       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:1775786/93‑1 (MQ=255)
        cggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGcc                                                                                 <  1:1151342/93‑1 (MQ=255)
        cggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGcc                                                                                 <  2:6549567/93‑1 (MQ=255)
          gcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGt                                                                               >  1:5395079/1‑93 (MQ=255)
            gTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAAATGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTgg                                                                             >  2:1190242/1‑93 (MQ=255)
             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            >  2:3927955/1‑93 (MQ=255)
             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            <  2:2196929/93‑1 (MQ=255)
              ggAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAc                                                                           <  2:2293470/93‑1 (MQ=255)
              ggAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAc                                                                           >  1:5765240/1‑93 (MQ=255)
               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          >  2:862200/1‑93 (MQ=255)
               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          <  2:3667090/93‑1 (MQ=255)
               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          <  2:5943743/93‑1 (MQ=255)
               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          >  1:4089224/1‑93 (MQ=255)
                aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         <  2:2723405/93‑1 (MQ=255)
                aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         <  1:4311216/93‑1 (MQ=255)
                  ccTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTc                                                                       <  2:3795951/93‑1 (MQ=255)
                  ccTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTc                                                                       <  1:1580059/93‑1 (MQ=255)
                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  2:5375920/1‑93 (MQ=255)
                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     <  1:2645729/93‑1 (MQ=255)
                     ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGACCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    >  2:1446769/1‑93 (MQ=255)
                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   <  2:3411298/93‑1 (MQ=255)
                       cgaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCga                                                                  <  2:2014982/93‑1 (MQ=255)
                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 <  1:4025528/93‑1 (MQ=255)
                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 >  1:481294/1‑93 (MQ=255)
                           cgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatgat                                                              <  1:4277858/93‑1 (MQ=255)
                            gaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGata                                                             <  1:5107730/93‑1 (MQ=255)
                            gaaTTCAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGata                                                             >  1:4332532/1‑93 (MQ=255)
                              aTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAAGAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAtt                                                           <  2:4997646/93‑1 (MQ=255)
                               ttGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTc                                                          >  2:2534180/1‑93 (MQ=255)
                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        >  1:1459298/1‑93 (MQ=255)
                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        <  2:3839339/93‑1 (MQ=255)
                                  aaGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCa                                                       >  2:3136751/1‑93 (MQ=255)
                                   agagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAc                                                      >  1:2716695/1‑93 (MQ=255)
                                    gagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAcc                                                     >  1:5176704/1‑93 (MQ=255)
                                      ggCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCaa                                                   >  1:3749793/1‑93 (MQ=255)
                                      ggCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCaa                                                   <  1:3238909/93‑1 (MQ=255)
                                       gCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAg                                                  <  1:351385/93‑1 (MQ=255)
                                         aCTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAc                                                >  1:6462700/1‑93 (MQ=255)
                                         aCTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAc                                                >  2:4407511/1‑93 (MQ=255)
                                           tACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGt                                              <  2:2477115/93‑1 (MQ=255)
                                            aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             >  2:1869856/1‑93 (MQ=255)
                                             cgccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAt                                            <  1:3086168/93‑1 (MQ=255)
                                              gccgcGAAATTCGCGAAGAGCTGGAAGAACAGCTGCTTTTGACAAAAATCCCGCCGTAGACCTTCAGCGATGATATTCGCACCAAGGCGTATg                                           <  2:6719223/93‑1 (MQ=255)
                                               ccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGc                                          >  2:3106511/1‑93 (MQ=255)
                                                 gcgAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAg                                        >  2:1028640/1‑93 (MQ=255)
                                                  cgAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGa                                       >  2:1596585/1‑93 (MQ=255)
                                                  cgAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGa                                       >  1:4711132/1‑93 (MQ=255)
                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  1:289610/93‑1 (MQ=255)
                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  1:5546479/93‑1 (MQ=255)
                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  2:5738581/93‑1 (MQ=255)
                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     >  2:3016356/1‑93 (MQ=255)
                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     >  1:1703243/1‑93 (MQ=255)
                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     >  2:75598/1‑93 (MQ=255)
                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   >  1:5645885/1‑93 (MQ=255)
                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   >  2:6612185/1‑93 (MQ=255)
                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  <  2:4742995/93‑1 (MQ=255)
                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  >  1:2324152/1‑93 (MQ=255)
                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  <  2:3055790/93‑1 (MQ=255)
                                                          gcgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCa                               >  2:1937953/1‑93 (MQ=255)
                                                          gcgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCa                               >  2:2571881/1‑93 (MQ=255)
                                                           cgAAGAACTGGGAGATCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:6713297/93‑1 (MQ=255)
                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:812914/93‑1 (MQ=255)
                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:1537903/93‑1 (MQ=255)
                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:492100/93‑1 (MQ=255)
                                                            gaagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAg                             <  1:1040727/93‑1 (MQ=255)
                                                             aagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAgg                            >  2:1061331/1‑93 (MQ=255)
                                                              agaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGa                           >  2:2039688/1‑93 (MQ=255)
                                                               gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGaa                          <  2:5550539/93‑1 (MQ=255)
                                                               gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGaa                          <  2:4350097/93‑1 (MQ=255)
                                                                 aCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAaga                        <  1:423645/93‑1 (MQ=255)
                                                                  cTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAagag                       >  2:3544045/1‑93 (MQ=255)
                                                                   tGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAgaga                      <  1:5444773/93‑1 (MQ=255)
                                                                    gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     >  1:5705769/1‑93 (MQ=255)
                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGGCCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  1:2969042/93‑1 (MQ=255)
                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  1:4995102/93‑1 (MQ=255)
                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    >  1:3421386/1‑93 (MQ=255)
                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  1:2571718/1‑93 (MQ=255)
                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  1:5924963/1‑93 (MQ=255)
                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                >  1:62993/1‑93 (MQ=255)
                                                                          aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               <  2:3240417/93‑1 (MQ=255)
                                                                          aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               >  1:3401901/1‑93 (MQ=255)
                                                                          aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               >  2:6087393/1‑93 (MQ=255)
                                                                           cAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATg              <  1:2852281/93‑1 (MQ=255)
                                                                           cAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATg              <  2:5054027/93‑1 (MQ=255)
                                                                            aGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGa             >  1:2170113/1‑93 (MQ=255)
                                                                             gctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAt            <  2:815698/93‑1 (MQ=255)
                                                                             gctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAt            <  1:6141174/93‑1 (MQ=255)
                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  2:62959/1‑93 (MQ=255)
                                                                                 ctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAc        <  1:729398/93‑1 (MQ=255)
                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:2248965/1‑93 (MQ=255)
                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAAAGATTTATATGATTTACCt      <  1:6564324/93‑1 (MQ=255)
                                                                                     tGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGa    <  1:4613887/93‑1 (MQ=255)
                                                                                      gACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAt   <  2:6143537/93‑1 (MQ=255)
                                                                                       aCAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAtt  <  1:1359898/93‑1 (MQ=255)
                                                                                       aCAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAtt  <  2:5088258/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  NC_000913/2362680‑2362862
                                                                                         |                                                                                             
CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAT                                                                                           <  SRR4302536.5749278/94‑1 (MQ=60)
   GTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCAC                                                                                        <  SRR4302536.6217390/94‑1 (MQ=60)
    TGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       <  SRR4302536.3612601/94‑1 (MQ=60)
    TGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       <  SRR4302536.2031175/94‑1 (MQ=60)
      GCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302536.2763935/94‑1 (MQ=60)
      GCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302536.478307/94‑1 (MQ=60)
       CGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCG                                                                                    <  SRR4302536.1186000/94‑1 (MQ=60)
        GGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGT                                                                                   >  SRR4302536.5556174/1‑94 (MQ=60)
          CGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGG                                                                                 >  SRR4302536.4817335/1‑94 (MQ=60)
          CGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGG                                                                                 >  SRR4302536.3146905/1‑94 (MQ=60)
            TGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAC                                                                               >  SRR4302536.5936764/1‑94 (MQ=60)
             GGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGCACAGCTGCTTTTGACAAAAATCACGCCGTGGACC                                                                              >  SRR4302536.6510732/1‑94 (MQ=60)
             GGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACC                                                                              >  SRR4302536.4210454/1‑94 (MQ=60)
              GAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCT                                                                             <  SRR4302536.516027/94‑1 (MQ=60)
               AGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTT                                                                            <  SRR4302536.4440226/94‑1 (MQ=60)
                 CCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCA                                                                          <  SRR4302536.1626344/94‑1 (MQ=60)
                 CCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCA                                                                          >  SRR4302536.5462420/1‑94 (MQ=60)
                   TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        <  SRR4302536.2725001/94‑1 (MQ=60)
                   TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        >  SRR4302536.721152/1‑94 (MQ=60)
                    GGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCG                                                                       <  SRR4302536.2199414/94‑1 (MQ=60)
                      CGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGAT                                                                     >  SRR4302536.493806/1‑94 (MQ=60)
                      CGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGACGTGGACCTTCAGCGAT                                                                     >  SRR4302536.5886846/1‑94 (MQ=60)
                       GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    >  SRR4302536.4437630/1‑94 (MQ=60)
                       GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    <  SRR4302536.4144940/94‑1 (MQ=60)
                        AACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGA                                                                   <  SRR4302536.2526220/94‑1 (MQ=60)
                        AACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGA                                                                   >  SRR4302536.5838461/1‑94 (MQ=60)
                          CGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATA                                                                 <  SRR4302536.243112/94‑1 (MQ=60)
                          CGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATA                                                                 <  SRR4302536.4405403/94‑1 (MQ=60)
                          CGAATTCAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATA                                                                 >  SRR4302536.4462293/1‑94 (MQ=60)
                           GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                <  SRR4302536.5260381/94‑1 (MQ=60)
                           GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                >  SRR4302536.3810878/1‑94 (MQ=60)
                            AATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATT                                                               >  SRR4302536.3057799/1‑94 (MQ=60)
                               TGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGC                                                            >  SRR4302536.1502489/1‑94 (MQ=60)
                               TGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGC                                                            >  SRR4302536.3483552/1‑94 (MQ=60)
                                 AAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAC                                                          <  SRR4302536.2941128/94‑1 (MQ=60)
                                 AAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAC                                                          >  SRR4302536.2797724/1‑94 (MQ=60)
                                 AAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAC                                                          >  SRR4302536.4401756/1‑94 (MQ=60)
                                  TGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACC                                                         >  SRR4302536.1749609/1‑94 (MQ=60)
                                  AGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACC                                                         >  SRR4302536.5331131/1‑94 (MQ=60)
                                    AGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAA                                                       >  SRR4302536.3861873/1‑94 (MQ=60)
                                    AGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGAAAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAA                                                       >  SRR4302536.5967490/1‑94 (MQ=60)
                                     GGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAG                                                      <  SRR4302536.2952754/94‑1 (MQ=60)
                                     GGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAG                                                      <  SRR4302536.5570246/94‑1 (MQ=60)
                                     GGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAG                                                      <  SRR4302536.3333838/94‑1 (MQ=60)
                                      GCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGA                                                     <  SRR4302536.360694/94‑1 (MQ=60)
                                      GCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGA                                                     <  SRR4302536.2349266/94‑1 (MQ=60)
                                       CACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAC                                                    >  SRR4302536.6655618/1‑94 (MQ=60)
                                       CACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAC                                                    >  SRR4302536.4025977/1‑94 (MQ=60)
                                        ACTCCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACG                                                   <  SRR4302536.6066187/94‑1 (MQ=60)
                                        ACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACG                                                   <  SRR4302536.3525750/94‑1 (MQ=60)
                                         CTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGT                                                  >  SRR4302536.5610284/1‑94 (MQ=60)
                                          TACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTA                                                 <  SRR4302536.762561/94‑1 (MQ=60)
                                          TACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTA                                                 >  SRR4302536.989009/1‑94 (MQ=60)
                                           ACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATTATATTCGCACCAAGACGTAT                                                <  SRR4302536.6734819/94‑1 (MQ=60)
                                           ACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAT                                                <  SRR4302536.272197/94‑1 (MQ=60)
                                           ACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAT                                                <  SRR4302536.3446235/94‑1 (MQ=60)
                                            CGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATG                                               <  SRR4302536.3176434/94‑1 (MQ=60)
                                               CGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAG                                            >  SRR4302536.1539267/1‑94 (MQ=60)
                                                GCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGA                                           >  SRR4302536.4852842/1‑94 (MQ=60)
                                                 CGAAATTCGCGAAGACCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          <  SRR4302536.629032/94‑1 (MQ=60)
                                                 CGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          >  SRR4302536.4496692/1‑94 (MQ=60)
                                                  GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         >  SRR4302536.1752527/1‑94 (MQ=60)
                                                  GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         <  SRR4302536.297307/94‑1 (MQ=60)
                                                  GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         <  SRR4302536.5711737/94‑1 (MQ=60)
                                                    AATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGT                                       >  SRR4302536.5813648/1‑94 (MQ=60)
                                                    AATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGT                                       <  SRR4302536.4209872/94‑1 (MQ=60)
                                                     ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      >  SRR4302536.2394681/1‑94 (MQ=60)
                                                     ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      >  SRR4302536.3512503/1‑94 (MQ=60)
                                                        CGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCA                                   <  SRR4302536.5638293/94‑1 (MQ=60)
                                                         GCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAA                                  <  SRR4302536.5375269/94‑1 (MQ=60)
                                                          CGAAGAACTGGGAGATCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAG                                 <  SRR4302536.6912603/94‑1 (MQ=60)
                                                          CGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAG                                 <  SRR4302536.1583128/94‑1 (MQ=60)
                                                          CGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAG                                 <  SRR4302536.6912602/94‑1 (MQ=60)
                                                          CGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAG                                 >  SRR4302536.5085489/1‑94 (MQ=60)
                                                          CGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAG                                 <  SRR4302536.504836/94‑1 (MQ=60)
                                                          CGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAG                                 <  SRR4302536.836761/94‑1 (MQ=60)
                                                           GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                <  SRR4302536.1071650/94‑1 (MQ=60)
                                                            AAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGA                               >  SRR4302536.1618894/1‑94 (MQ=60)
                                                            AAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGA                               >  SRR4302536.3234393/1‑94 (MQ=60)
                                                             AGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAA                              <  SRR4302536.4218168/94‑1 (MQ=60)
                                                             AGAACGGGGAGAACAGCTGCTTTTGACAAAAACCCCGCCGTGGCCCTTCAGCGATGATATTCGCACCAAGACGTATCCAGATGGTCGCAAGGAA                              <  SRR4302536.1149199/94‑1 (MQ=60)
                                                              GAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAG                             <  SRR4302536.4986205/94‑1 (MQ=60)
                                                              GAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAG                             <  SRR4302536.6403013/94‑1 (MQ=60)
                                                              GAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAG                             <  SRR4302536.1933572/94‑1 (MQ=60)
                                                               AACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGA                            >  SRR4302536.2081398/1‑94 (MQ=60)
                                                                ACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAG                           <  SRR4302536.434809/94‑1 (MQ=60)
                                                                ACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAG                           <  SRR4302536.2655833/94‑1 (MQ=60)
                                                                ACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAG                           <  SRR4302536.6884535/94‑1 (MQ=60)
                                                                 CTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGA                          <  SRR4302536.3039481/94‑1 (MQ=60)
                                                                  TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         <  SRR4302536.5607642/94‑1 (MQ=60)
                                                                  TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         <  SRR4302536.149301/94‑1 (MQ=60)
                                                                  TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         <  SRR4302536.1643043/94‑1 (MQ=60)
                                                                  TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         <  SRR4302536.6131106/94‑1 (MQ=60)
                                                                  TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         >  SRR4302536.4251164/1‑94 (MQ=60)
                                                                  TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         >  SRR4302536.5875440/1‑94 (MQ=60)
                                                                  TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         >  SRR4302536.5851489/1‑94 (MQ=60)
                                                                   TGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATT                        >  SRR4302536.3523207/1‑94 (MQ=60)
                                                                   GGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGTACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATT                        <  SRR4302536.1116166/94‑1 (MQ=60)
                                                                   GGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATT                        >  SRR4302536.44782/1‑94 (MQ=60)
                                                                    GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGGCCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       <  SRR4302536.3056498/94‑1 (MQ=60)
                                                                    GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       <  SRR4302536.6316875/94‑1 (MQ=60)
                                                                    GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       <  SRR4302536.1253575/94‑1 (MQ=60)
                                                                    GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       <  SRR4302536.1561714/94‑1 (MQ=60)
                                                                    GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       <  SRR4302536.5145032/94‑1 (MQ=60)
                                                                      AGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTAT                     >  SRR4302536.2649051/1‑94 (MQ=60)
                                                                      AGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTAT                     >  SRR4302536.835839/1‑94 (MQ=60)
                                                                      AGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTAT                     >  SRR4302536.6101458/1‑94 (MQ=60)
                                                                       GAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATA                    >  SRR4302536.64715/1‑94 (MQ=60)
                                                                        AACAGCTGCTTTTGACAAAAATCACGCCGTGGCCCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGTAAGAGATTTATAT                   <  SRR4302536.4456883/94‑1 (MQ=60)
                                                                        AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   >  SRR4302536.3503077/1‑94 (MQ=60)
                                                                        AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   >  SRR4302536.5321954/1‑94 (MQ=60)
                                                                          CAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGA                 >  SRR4302536.2235449/1‑94 (MQ=60)
                                                                          CAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGA                 >  SRR4302536.2918586/1‑94 (MQ=60)
                                                                          CAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGA                 <  SRR4302536.2936594/94‑1 (MQ=60)
                                                                           AGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAT                >  SRR4302536.4755104/1‑94 (MQ=60)
                                                                           AGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAT                <  SRR4302536.5149139/94‑1 (MQ=60)
                                                                           AGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAT                <  SRR4302536.6622401/94‑1 (MQ=60)
                                                                            GCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATT               <  SRR4302536.6324180/94‑1 (MQ=60)
                                                                            GCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATT               >  SRR4302536.4826083/1‑94 (MQ=60)
                                                                             CTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTT              <  SRR4302536.4913487/94‑1 (MQ=60)
                                                                              TGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTA             >  SRR4302536.5038037/1‑94 (MQ=60)
                                                                                CTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACC           <  SRR4302536.750770/94‑1 (MQ=60)
                                                                                  TTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTG         <  SRR4302536.132151/94‑1 (MQ=60)
                                                                                  TTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTG         >  SRR4302536.397512/1‑94 (MQ=60)
                                                                                  TTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAAAGATTTATATGATTTACCTG         <  SRR4302536.6759719/94‑1 (MQ=60)
                                                                                    TGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       <  SRR4302536.4752486/94‑1 (MQ=60)
                                                                                     GACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT      <  SRR4302536.863061/94‑1 (MQ=60)
                                                                                     GACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT      <  SRR4302536.4421450/94‑1 (MQ=60)
                                                                                      ACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTT     <  SRR4302536.1400423/94‑1 (MQ=60)
                                                                                       CAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTT    <  SRR4302536.5238559/94‑1 (MQ=60)
                                                                                        AAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   <  SRR4302536.1404090/94‑1 (MQ=60)
                                                                                        AAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   >  SRR4302536.76719/1‑94 (MQ=60)
                                                                                         AAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  SRR4302536.6358961/1‑94 (MQ=60)
                                                                                         AAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  SRR4302536.5567230/1‑94 (MQ=60)
                                                                                         |                                                                                             
CGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  NC_000913/2362680‑2362862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑