Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,375,871 T→C N470S (AAC→AGC)  menD ← 2‑oxoglutarate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,8710TC99.2% 398.9 / ‑4.4 126N470S (AAC→AGC) menD2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (62/63);  total (63/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 141 total aligned reads displayed.

GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960
                                                                                          |                                                                                         
 cgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgt                                                                                        >  2:2334316/1‑93 (MQ=255)
  gTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgtt                                                                                       >  2:1286661/1‑93 (MQ=255)
   tGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttg                                                                                      >  1:820904/1‑93 (MQ=255)
   tGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttg                                                                                      >  1:333127/1‑93 (MQ=255)
   tGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttg                                                                                      >  1:3608663/1‑93 (MQ=255)
    gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgt                                                                                     >  1:4249211/1‑93 (MQ=255)
    gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgt                                                                                     <  2:4585942/93‑1 (MQ=255)
     cTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgtt                                                                                    <  1:1635382/93‑1 (MQ=255)
      tCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTc                                                                                   >  1:1061074/1‑93 (MQ=255)
       cAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGATAAAATTTGCCCGCCGCTGTTGTTca                                                                                  <  1:2706341/93‑1 (MQ=255)
         aaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcacc                                                                                >  2:239111/1‑93 (MQ=255)
         aaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcacc                                                                                >  1:1685654/1‑93 (MQ=255)
         aaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcacc                                                                                >  1:3543901/1‑93 (MQ=255)
             ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACaa                                                                            >  1:2005710/1‑93 (MQ=255)
             ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACaa                                                                            <  2:3975646/93‑1 (MQ=255)
              gACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAt                                                                           >  2:549769/1‑93 (MQ=255)
              gACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAt                                                                           >  2:3681531/1‑93 (MQ=255)
               aCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAtt                                                                          >  1:3594588/1‑93 (MQ=255)
               aCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAtt                                                                          >  1:562514/1‑93 (MQ=255)
                cGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTa                                                                         >  1:759481/1‑93 (MQ=255)
                 gTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTaa                                                                        <  1:1096373/93‑1 (MQ=255)
                  ttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAt                                                                       <  2:2490469/93‑1 (MQ=255)
                  ttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAt                                                                       <  2:1136508/93‑1 (MQ=255)
                  ttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAt                                                                       >  1:887728/1‑93 (MQ=255)
                   tttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATa                                                                      <  1:2465298/93‑1 (MQ=255)
                      gCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCa                                                                   <  1:2285673/93‑1 (MQ=255)
                        ggCATCAAATAGAAACGCTCACGCTCGCTTTGCGTCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgc                                                                 <  1:3239410/93‑1 (MQ=255)
                         gCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcg                                                                >  1:4414411/1‑93 (MQ=255)
                           aTCAGATAGAACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcggc                                                              <  1:884846/93‑1 (MQ=255)
                            tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcggcg                                                             <  1:798441/93‑1 (MQ=255)
                             cAGATAGAAACCCTCCCGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGgcgc                                                            <  1:908694/93‑1 (MQ=255)
                              aGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCa                                                           >  1:2673877/1‑93 (MQ=255)
                               gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCATCACAATTAATACCAGCGGCGCAg                                                          >  2:2584311/1‑93 (MQ=255)
                               gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCaa                                                          >  1:3360787/1‑92 (MQ=255)
                               gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAg                                                          <  1:2476366/93‑1 (MQ=255)
                               gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAg                                                          >  1:750829/1‑93 (MQ=255)
                                aTAGAAACGCGCACGCCCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGa                                                         <  2:4377433/93‑1 (MQ=255)
                                  aGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  1:1738209/93‑1 (MQ=255)
                                  aGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  2:1335163/93‑1 (MQ=255)
                                  aGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  1:449155/93‑1 (MQ=255)
                                   gAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAc                                                      >  2:2638568/1‑93 (MQ=255)
                                    aaaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAcc                                                     <  2:2534531/93‑1 (MQ=255)
                                    aaaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAcc                                                     >  2:2155153/1‑93 (MQ=255)
                                     aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCt                                                    <  1:4131038/93‑1 (MQ=255)
                                      acgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTg                                                   <  2:3660352/93‑1 (MQ=255)
                                       cgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGa                                                  >  2:4414378/1‑93 (MQ=255)
                                        gctcccgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                 <  2:605005/93‑1 (MQ=255)
                                        gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                 >  2:2019117/1‑93 (MQ=255)
                                        gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                 <  1:2371604/93‑1 (MQ=255)
                                        gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                 >  1:136148/1‑93 (MQ=255)
                                          tcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGc                                               >  2:2602322/1‑93 (MQ=255)
                                            ccgctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCaa                                             <  2:125475/92‑1 (MQ=255)
                                            acgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCaa                                             >  2:2066594/1‑93 (MQ=255)
                                             cgctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAAt                                            >  2:3033722/1‑93 (MQ=255)
                                              gctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATa                                           >  1:4170893/1‑93 (MQ=255)
                                              gctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATa                                           >  1:3533376/1‑93 (MQ=255)
                                              gctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATa                                           <  1:3475108/93‑1 (MQ=255)
                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  2:4567108/93‑1 (MQ=255)
                                               ctcgctTTGAGGCGTGGCCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  2:2587737/93‑1 (MQ=255)
                                               cgcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  2:1910305/91‑1 (MQ=255)
                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                         >  1:3182776/1‑93 (MQ=255)
                                                  gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGc                                       >  2:4423918/1‑93 (MQ=255)
                                                   ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGcc                                      <  2:1476771/93‑1 (MQ=255)
                                                    tttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCCCAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa                                     >  1:1008597/1‑93 (MQ=255)
                                                    tttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa                                     >  2:3127353/1‑93 (MQ=255)
                                                    tttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa                                     >  1:1008598/1‑93 (MQ=255)
                                                    tttGCGGCGTTGGAAACGGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa                                     <  2:1710534/93‑1 (MQ=255)
                                                      tGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgc                                   >  2:3192298/1‑93 (MQ=255)
                                                      tGCGGCGTTGGCAACAGCGAGAAAATTGGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgc                                   <  2:4352283/93‑1 (MQ=255)
                                                       gcggcgTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcg                                  <  2:418032/93‑1 (MQ=255)
                                                        cggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATCCCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc                                 <  2:466143/93‑1 (MQ=255)
                                                        cggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc                                 >  1:2568341/1‑93 (MQ=255)
                                                          gcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt                               <  2:4458966/93‑1 (MQ=255)
                                                           cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              <  2:2332437/93‑1 (MQ=255)
                                                               ggCAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          <  2:3746253/93‑1 (MQ=255)
                                                                gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                         <  1:3589317/93‑1 (MQ=255)
                                                                 cAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATc                        <  1:1727721/93‑1 (MQ=255)
                                                                  aaCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCg                       >  2:3205735/1‑93 (MQ=255)
                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      >  1:4738635/1‑93 (MQ=255)
                                                                     aGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaa                    >  2:2858257/1‑93 (MQ=255)
                                                                      gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                   <  2:279394/93‑1 (MQ=255)
                                                                        gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAgg                 >  1:330863/1‑92 (MQ=255)
                                                                            aaaTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg             >  2:2292348/1‑93 (MQ=255)
                                                                             aaTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGga            >  1:3735948/1‑93 (MQ=255)
                                                                              aTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGGAAAGTGCGgag           >  2:4322059/1‑93 (MQ=255)
                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgaga          >  2:1013903/1‑93 (MQ=255)
                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAGCGTAAAGTGCGgagg          >  2:604304/1‑92 (MQ=255)
                                                                                tttcccGCCGCTGTTGTTCAACACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagag         <  1:303975/90‑1 (MQ=255)
                                                                                 tGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga        <  2:2709303/93‑1 (MQ=255)
                                                                                 tGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga        <  2:880569/93‑1 (MQ=255)
                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       <  1:1173526/93‑1 (MQ=255)
                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       <  2:924333/93‑1 (MQ=255)
                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       <  2:3867941/93‑1 (MQ=255)
                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       >  1:573279/1‑93 (MQ=255)
                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       <  2:2814543/93‑1 (MQ=255)
                                                                                   cccGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc      <  1:4021207/93‑1 (MQ=255)
                                                                                   cccGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc      <  2:1936542/93‑1 (MQ=255)
                                                                                    ccgccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCg     >  2:1965471/1‑93 (MQ=255)
                                                                                     cgccgcTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAGGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGc    >  2:3599825/1‑93 (MQ=255)
                                                                                     cgccgcTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGc    >  2:736242/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACA  >  NC_000913/2375779‑2375963
                                                                                            |                                                                                            
CGGGGTGCTCAAAAGGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCT                                                                                             <  SRR4302544.4137666/94‑1 (MQ=60)
CGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCT                                                                                             >  SRR4302544.276567/1‑94 (MQ=60)
  TCGTGCTAAAAATGGAGGTTGTCCGGCATCAGAGAGAAACGTGCACGCTCGCTGTCCGGCGTTGCAAACAGCGAGAAAATTTGCCCGCCGCTGT                                                                                           <  SRR4302544.8906/94‑1 (MQ=23)
  GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGT                                                                                           <  SRR4302544.8907/94‑1 (MQ=60)
    GTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTG                                                                                         >  SRR4302544.3643826/1‑94 (MQ=60)
    GTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTG                                                                                         >  SRR4302544.336590/1‑94 (MQ=60)
    GTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTG                                                                                         >  SRR4302544.828931/1‑94 (MQ=60)
     TGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGT                                                                                        >  SRR4302544.4292534/1‑94 (MQ=60)
      GCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTT                                                                                       <  SRR4302544.596319/94‑1 (MQ=60)
       CTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTC                                                                                      >  SRR4302544.1071613/1‑94 (MQ=60)
       CTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTC                                                                                      <  SRR4302544.1651480/94‑1 (MQ=60)
         CAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGATAAAATTTGCCCGCCGCTGTTGTTCAC                                                                                    <  SRR4302544.2732364/94‑1 (MQ=60)
          AAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACC                                                                                   >  SRR4302544.3577832/1‑94 (MQ=60)
          AAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACC                                                                                   >  SRR4302544.1702268/1‑94 (MQ=60)
          AAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACC                                                                                   >  SRR4302544.3537996/1‑94 (MQ=60)
              TGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAA                                                                               >  SRR4302544.2025194/1‑94 (MQ=60)
                GACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATT                                                                             >  SRR4302544.568014/1‑94 (MQ=60)
                GACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATT                                                                             >  SRR4302544.3629131/1‑94 (MQ=60)
                 ACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTA                                                                            >  SRR4302544.766890/1‑94 (MQ=60)
                  CGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAA                                                                           <  SRR4302544.4404765/94‑1 (MQ=60)
                   GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAT                                                                          <  SRR4302544.1107281/94‑1 (MQ=60)
                   GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAT                                                                          >  SRR4302544.896528/1‑94 (MQ=60)
                     TTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATAC                                                                        <  SRR4302544.2488844/94‑1 (MQ=60)
                        GCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAG                                                                     <  SRR4302544.2307218/94‑1 (MQ=60)
                         CGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGC                                                                    <  SRR4302544.584728/94‑1 (MQ=60)
                          GGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCG                                                                   >  SRR4302544.4458979/1‑94 (MQ=60)
                          GGCATCAAATAGAAACGCTCACGCTCGCTTTGCGTCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCG                                                                   <  SRR4302544.3270416/94‑1 (MQ=60)
                            CATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGC                                                                 <  SRR4302544.3216416/94‑1 (MQ=60)
                             gtcacatagaagcgctcaggctccggttgggcacTTGGCAACCGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCG                                                                <  SRR4302544.616297/60‑1 (MQ=60)
                             ATCAGATAGAACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCG                                                                <  SRR4302544.893612/94‑1 (MQ=60)
                              TCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGC                                                               <  SRR4302544.806183/94‑1 (MQ=60)
                               CAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCA                                                              >  SRR4302544.2699509/1‑94 (MQ=60)
                               CAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCA                                                              <  SRR4302544.781950/94‑1 (MQ=60)
                               CAGATAGAAACCCTCCCGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCA                                                              <  SRR4302544.917675/94‑1 (MQ=60)
                                AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAG                                                             >  SRR4302544.758163/1‑94 (MQ=60)
                                AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAA                                                             >  SRR4302544.3392861/1‑94 (MQ=60)
                                 GATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGA                                                            >  SRR4302544.3351371/1‑94 (MQ=60)
                                 GATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGA                                                            <  SRR4302544.2500052/94‑1 (MQ=60)
                                    AGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAC                                                         <  SRR4302544.453617/94‑1 (MQ=60)
                                    AGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAC                                                         <  SRR4302544.1755231/94‑1 (MQ=60)
                                      AAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCT                                                       <  SRR4302544.2010947/94‑1 (MQ=60)
                                       AACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTG                                                      <  SRR4302544.4171988/94‑1 (MQ=60)
                                         CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAC                                                    >  SRR4302544.137434/1‑94 (MQ=60)
                                          GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACG                                                   <  SRR4302544.251602/94‑1 (MQ=60)
                                          GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACG                                                   <  SRR4302544.2394048/94‑1 (MQ=60)
                                           CTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGC                                                  >  SRR4302544.2896882/1‑94 (MQ=60)
                                            TCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCA                                                 <  SRR4302544.245131/94‑1 (MQ=60)
                                               CGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATA                                              >  SRR4302544.3567176/1‑94 (MQ=60)
                                               CGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATA                                              >  SRR4302544.4212211/1‑94 (MQ=60)
                                                GCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAA                                             <  SRR4302544.3508250/94‑1 (MQ=60)
                                                 CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAC                                            >  SRR4302544.3213065/1‑94 (MQ=60)
                                                 CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAC                                            <  SRR4302544.1009869/94‑1 (MQ=60)
                                                  TCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCTGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACG                                           <  SRR4302544.4717994/94‑1 (MQ=60)
                                                   CGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGC                                          >  SRR4302544.1732268/1‑94 (MQ=60)
                                                     CTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCCCAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                        >  SRR4302544.1018630/1‑94 (MQ=60)
                                                     CTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                        >  SRR4302544.3754340/1‑94 (MQ=60)
                                                     CTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                        >  SRR4302544.1018631/1‑94 (MQ=60)
                                                         GCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGC                                    >  SRR4302544.2593015/1‑94 (MQ=60)
                                                                  GCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATC                           <  SRR4302544.3623784/94‑1 (MQ=60)
                                                                   CAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCG                          <  SRR4302544.1744664/94‑1 (MQ=60)
                                                                    AACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGT                         >  SRR4302544.4785340/1‑94 (MQ=60)
                                                                      CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                       >  SRR4302544.1091366/1‑94 (MQ=60)
                                                                      CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACCCAATAACGCCAACGCGTTGAGATCGTAA                       >  SRR4302544.1326457/1‑94 (MQ=60)
                                                                         CGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGT                    >  SRR4302544.3621850/1‑94 (MQ=60)
                                                                         CGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGG                    >  SRR4302544.334305/1‑94 (MQ=60)
                                                                              AAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGA               >  SRR4302544.3773087/1‑94 (MQ=60)
                                                                                  TTTCCCGCCGCTGTTGTTCAACACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGA           <  SRR4302544.307128/94‑1 (MQ=60)
                                                                                   TGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAT          >  SRR4302544.578886/1‑94 (MQ=60)
                                                                                    GCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC         <  SRR4302544.1185235/94‑1 (MQ=60)
                                                                                     CCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCG        <  SRR4302544.4061231/94‑1 (MQ=60)
                                                                                         CCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCA    <  SRR4302544.409250/94‑1 (MQ=60)
                                                                                          CGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCAC   >  SRR4302544.3914545/1‑94 (MQ=60)
                                                                                          CGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCAC   >  SRR4302544.379633/1‑94 (MQ=60)
                                                                                           GCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCGACA  >  SRR4302544.4289348/1‑94 (MQ=60)
                                                                                            |                                                                                            
CGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACA  >  NC_000913/2375779‑2375963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑