Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,871	T→C	N470S (AAC→AGC)	menD ←	2‑oxoglutarate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,871	0	T	C	100.0%	689.4 / NA	213	N470S (AAC→AGC)	menD	2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (101/112); total (101/112)

Only 100 of 238 total aligned reads displayed.

GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960

gcgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctg                                                                                         <  2:2714553/93‑1 (MQ=255)

gcgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctg                                                                                         <  1:3973941/93‑1 (MQ=255)

  gTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgtt                                                                                       <  1:6097838/93‑1 (MQ=255)

    gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgt                                                                                     >  2:2092202/1‑93 (MQ=255)

    gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgt                                                                                     >  2:5542507/1‑93 (MQ=255)

     cTCAAAATGGACGTTTTGCGGCATCAGATAGAACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgtt                                                                                    <  2:4109726/93‑1 (MQ=255)

     cTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgtt                                                                                    >  2:2509343/1‑93 (MQ=255)

      tCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTc                                                                                   >  2:4946303/1‑93 (MQ=255)

      tCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTc                                                                                   >  1:4209126/1‑93 (MQ=255)

        aaaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcac                                                                                 >  2:1620159/1‑93 (MQ=255)

         aaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcacc                                                                                <  2:3779202/93‑1 (MQ=255)

           aTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcaccac                                                                              >  1:1007996/1‑93 (MQ=255)

           aTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcaccac                                                                              >  2:1600900/1‑93 (MQ=255)

           aTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcaccac                                                                              <  2:6508464/93‑1 (MQ=255)

             ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACaa                                                                            <  2:5910424/93‑1 (MQ=255)

               aCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAtt                                                                          >  1:5813946/1‑93 (MQ=255)

               aCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAtt                                                                          >  2:885578/1‑93 (MQ=255)

                 gTTTTGCGGCATCAGATAGAATCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTaa                                                                        <  1:3514165/93‑1 (MQ=255)

                  ttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAt                                                                       >  1:1599504/1‑93 (MQ=255)

                   tttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATa                                                                      <  2:6320494/93‑1 (MQ=255)

                   tttGCGGCATCAGATAGAAACGCCCACGCTCGCTTTGCGGCGTGGGCAAAAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATa                                                                      <  2:1841178/93‑1 (MQ=255)

                   tttGCGGCACCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATa                                                                      <  1:797238/93‑1 (MQ=255)

                     tGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATAcc                                                                    <  2:5141599/93‑1 (MQ=255)

                      gCGGCATCAGATAGAACCGCTCACGCTCGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCa                                                                   <  2:3667555/93‑1 (MQ=255)

                        ggCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgc                                                                 >  2:671858/1‑93 (MQ=255)

                         gCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcg                                                                <  2:2362914/93‑1 (MQ=255)

                         gCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcg                                                                <  2:5684761/93‑1 (MQ=255)

                          cATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcgg                                                               >  2:6102200/1‑93 (MQ=255)

                          cATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcgg                                                               >  1:5187194/1‑93 (MQ=255)

                           aTCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcggc                                                              >  1:4922419/1‑93 (MQ=255)

                            tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAgcggcg                                                             >  2:1042146/1‑93 (MQ=255)

                             cAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGgcgc                                                            <  1:5062630/93‑1 (MQ=255)

                              aGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCa                                                           >  1:1378077/1‑93 (MQ=255)

                                  aGAACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  2:5957/93‑1 (MQ=255)

                                  aGAACCCCTCACGCTCGCTTGGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  1:1255210/93‑1 (MQ=255)

                                  aGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGTCCGCCGCTGTTGTTCACCACAATTCATACCAGCGGCGCAGaaa                                                       >  1:3260172/1‑93 (MQ=255)

                                  aGAAACGCTCACGCTCCCTTTGTGGCGTAGGCAACAGCGATAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                       <  1:528537/93‑1 (MQ=255)

                                    aaccgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAcc                                                     <  1:2426223/90‑1 (MQ=255)

                                     accgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCt                                                    <  1:605801/91‑1 (MQ=255)

                                     aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCt                                                    >  2:6265534/1‑93 (MQ=255)

                                     aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCt                                                    <  1:1941581/93‑1 (MQ=255)

                                         ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACg                                                >  1:1086448/1‑93 (MQ=255)

                                          tcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGc                                               >  2:2194028/1‑93 (MQ=255)

                                             cgctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAGTACCAGCGGGGCAGAAACCTGAGGCAAt                                            >  1:5309717/1‑93 (MQ=255)

                                             cgctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAAt                                            <  1:2799977/93‑1 (MQ=255)

                                              gctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATa                                           >  2:5549235/1‑93 (MQ=255)

                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  2:1408971/93‑1 (MQ=255)

                                               ctcgctTGGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  1:3542807/93‑1 (MQ=255)

                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                         >  1:5090979/1‑93 (MQ=255)

                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                         >  1:1157803/1‑93 (MQ=255)

                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                         <  1:3107300/93‑1 (MQ=255)

                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTAACCACAATTAATACCAGCGGCGACGAAACCTGACGCAATAAc                                         >  1:3402484/1‑93 (MQ=255)

                                                  gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGc                                       >  2:5608445/1‑93 (MQ=255)

                                                   ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATCACGcc                                      >  1:1946613/1‑93 (MQ=255)

                                                    tttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATACCGCCa                                     >  2:814675/1‑93 (MQ=255)

                                                    tttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa                                     >  1:2262475/1‑93 (MQ=255)

                                                     ttGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                    <  2:90017/93‑1 (MQ=255)

                                                          gcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt                               >  1:1033925/1‑93 (MQ=255)

                                                          gcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt                               <  1:843058/93‑1 (MQ=255)

                                                           ggTTGGAAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              <  2:4247919/92‑1 (MQ=255)

                                                           cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              <  2:1934989/93‑1 (MQ=255)

                                                           cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              <  1:2702279/93‑1 (MQ=255)

                                                            gTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTg                             <  1:5621285/93‑1 (MQ=255)

                                                             ttGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                            >  1:4444515/1‑93 (MQ=255)

                                                              tGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgag                           >  2:4735698/1‑93 (MQ=255)

                                                               ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          <  2:4385231/93‑1 (MQ=255)

                                                               ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          >  1:2947351/1‑93 (MQ=255)

                                                               ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          >  2:2849196/1‑93 (MQ=255)

                                                               ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          <  2:5403325/93‑1 (MQ=255)

                                                                gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgagac                         >  2:4482649/1‑92 (MQ=255)

                                                                  aaCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCg                       <  2:638728/93‑1 (MQ=255)

                                                                  aaCAGAGAAAAAATTTGCCCGCCGCTTTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCg                       <  2:1353651/93‑1 (MQ=255)

                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      >  2:3433463/1‑93 (MQ=255)

                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      >  2:3237790/1‑93 (MQ=255)

                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      >  2:2409892/1‑93 (MQ=255)

                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      >  2:4409687/1‑93 (MQ=255)

                                                                     aGCGAGAAACTTTCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaa                    <  2:2117180/93‑1 (MQ=255)

                                                                     aGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaa                    <  2:5063344/93‑1 (MQ=255)

                                                                     aGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaa                    >  1:2149825/1‑93 (MQ=255)

                                                                      gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                   >  1:6323045/1‑93 (MQ=255)

                                                                       gaagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  <  2:3889245/91‑1 (MQ=255)

                                                                       cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  <  2:1333695/93‑1 (MQ=255)

                                                                        gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                 >  2:1495518/1‑93 (MQ=255)

                                                                         agaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTg                <  1:1996800/93‑1 (MQ=255)

                                                                         agaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTg                <  1:3719725/93‑1 (MQ=255)

                                                                          gaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc               >  1:6356988/1‑93 (MQ=255)

                                                                            aattttGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg             <  1:1496741/90‑1 (MQ=255)

                                                                            aaaTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg             >  1:5539075/1‑93 (MQ=255)

                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTCACGCAATAACGCCAGCGCGCTGAGATCGTAAAGTGCGgaga          >  1:4516750/1‑93 (MQ=255)

                                                                                 tGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga        <  2:6349674/93‑1 (MQ=255)

                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       <  1:4304884/93‑1 (MQ=255)

                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       >  2:6627780/1‑93 (MQ=255)

                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       <  2:3456376/93‑1 (MQ=255)

                                                                                  gCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt       >  1:6417807/1‑93 (MQ=255)

                                                                                   cccGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc      <  2:1201/93‑1 (MQ=255)

                                                                                    ccgccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCg     <  1:5540986/93‑1 (MQ=255)

                                                                                    ccgccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCg     >  2:5102243/1‑93 (MQ=255)

                                                                                       ccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGccc  >  1:3796701/1‑93 (MQ=255)

                                                                                       ccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGccc  <  1:4391868/93‑1 (MQ=255)

                                                                                       ccgCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGccc  >  1:4552318/1‑93 (MQ=255)

GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACA  >  NC_000913/2375778‑2375963

GCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGC                                                                                              <  SRR4302536.1957864/94‑1 (MQ=60)

GCGG‑GTGCTCAAAATGGACGTTTTGCGGAATCAGATAGAACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCT                                                                                             <  SRR4302536.4167402/94‑1 (MQ=60)

 GGGCGTGCTCAAAATGGACTTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCT                                                                                             <  SRR4302536.2701284/94‑1 (MQ=60)

   GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGT                                                                                           <  SRR4302536.4091899/94‑1 (MQ=60)

    CGTGCTCAAATTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTT                                                                                          <  SRR4302536.4677202/94‑1 (MQ=60)

    CGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTT                                                                                          <  SRR4302536.4380580/94‑1 (MQ=60)

     GTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTG                                                                                         <  SRR4302536.6279663/94‑1 (MQ=60)

      TGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGT                                                                                        <  SRR4302536.6148503/94‑1 (MQ=60)

       CCTCAAAATGGCCTTTTTGCGGCATCAGATAGAAACCCTCCCGCTCGCTTCGCGCAGTTGCCAACAGCGAGAAAT‑TTTGCCCGCCGCTGTTGTT                                                                                       <  SRR4302536.38968/94‑1 (MQ=40)

        CTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTC                                                                                      >  SRR4302536.4334239/1‑94 (MQ=60)

         TCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCA                                                                                     >  SRR4302536.6550708/1‑94 (MQ=60)

         CCAAAATGGCCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGGGAAAA‑TTTGCCCGCCGCTGTTGTTCA                                                                                     <  SRR4302536.1534243/94‑1 (MQ=60)

          CAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCAC                                                                                    >  SRR4302536.3623393/1‑94 (MQ=60)

             AATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCAC                                                                                 >  SRR4302536.1037725/1‑94 (MQ=60)

              ATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACA                                                                                <  SRR4302536.5467640/94‑1 (MQ=60)

              ATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACA                                                                                >  SRR4302536.1137453/1‑94 (MQ=60)

              ATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACA                                                                                <  SRR4302536.3464269/94‑1 (MQ=60)

                GGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAAT                                                                              <  SRR4302536.2951634/94‑1 (MQ=60)

                GGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAAT                                                                              >  SRR4302536.1004969/1‑94 (MQ=60)

                GCACGTTTTGCGCCATCAGATAGAATCGCTCACCCTCGCTTTGCTGCGTTGTCACCGGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAAT                                                                              <  SRR4302536.3275718/94‑1 (MQ=60)

                 GACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATT                                                                             >  SRR4302536.5987138/1‑94 (MQ=60)

                   CGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAA                                                                           >  SRR4302536.1118977/1‑94 (MQ=60)

                    GTTTTGCGGCATCAGATAGAATCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAAT                                                                          <  SRR4302536.3619101/94‑1 (MQ=60)

                    GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAAT                                                                          >  SRR4302536.1646207/1‑94 (MQ=60)

                    GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAAT                                                                          >  SRR4302536.658483/1‑94 (MQ=60)

                      TTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATAC                                                                        <  SRR4302536.2833250/94‑1 (MQ=60)

                      TTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATAC                                                                        <  SRR4302536.5917363/94‑1 (MQ=60)

                      TTTGCGGCACCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATAC                                                                        <  SRR4302536.820604/94‑1 (MQ=60)

                       TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACC                                                                       <  SRR4302536.3305044/94‑1 (MQ=60)

                       TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACC                                                                       <  SRR4302536.2403793/94‑1 (MQ=60)

                         GCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAG                                                                     <  SRR4302536.4184169/94‑1 (MQ=60)

                          CGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGC                                                                    <  SRR4302536.2520539/94‑1 (MQ=60)

                           GGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCG                                                                   >  SRR4302536.5754368/1‑94 (MQ=60)

                            GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGG                                                                  <  SRR4302536.5633283/94‑1 (MQ=60)

                            GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGG                                                                  >  SRR4302536.5341909/1‑94 (MQ=60)

                             CATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGC                                                                 >  SRR4302536.5070339/1‑94 (MQ=60)

                             CATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGC                                                                 >  SRR4302536.3891205/1‑94 (MQ=60)

                              ATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCG                                                                >  SRR4302536.2995989/1‑94 (MQ=60)

                               TCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCCGCGC                                                               >  SRR4302536.1492716/1‑94 (MQ=60)

                                CAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCGACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCA                                                              >  SRR4302536.4509879/1‑94 (MQ=60)

                                CAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCA                                                              >  SRR4302536.1419131/1‑94 (MQ=60)

                                CAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCA                                                              <  SRR4302536.5214267/94‑1 (MQ=60)

                                 AGATAGAAACGCTCTCGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAT‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAG                                                             <  SRR4302536.3707843/94‑1 (MQ=60)

                                 AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAG                                                             >  SRR4302536.3950929/1‑94 (MQ=60)

                                    TAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGTCCGCCGCTGTTGTTCACCACAATTCATACCAGCGGCGCAGAAA                                                          >  SRR4302536.3355758/1‑94 (MQ=60)

                                    TAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAA                                                          >  SRR4302536.2112212/1‑94 (MQ=60)

                                     AGAACCCCTCACGCTCGCTTGGCGGCGTTGGCAACAGCGAAAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAC                                                         <  SRR4302536.1292884/94‑1 (MQ=60)

                                     AGAAACGCTCACGCTCGCTTTGCGTCGTTGGCGACAGCGAGAAAA‑TTTGCCCGCCGCTGTTCTTCACCACAATTAATACCACGGGCGCAGAACC                                                         >  SRR4302536.4618583/1‑94 (MQ=60)

                                     AGAAACGCTCACGCTCCCTTTGTGGCGTAGGCAACAGCGATAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAAC                                                         <  SRR4302536.542057/94‑1 (MQ=60)

                                       AACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCT                                                       <  SRR4302536.2499583/94‑1 (MQ=60)

                                       AAACGCTCCCGCTCGCTTTGCGGCGTTGGCAACAGCGATAAAA‑TTTTCCCGCCGCTGTTGTTCACCCCAATTAATACCAGCGGCGCCGAAACCT                                                       >  SRR4302536.2778092/1‑94 (MQ=60)

                                       AAACGCTCCCCCTCGCTTTGCGCCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCT                                                       <  SRR4302536.3719289/94‑1 (MQ=60)

                                       AAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCT                                                       <  SRR4302536.1649291/94‑1 (MQ=60)

                                       AAACGCTCACGCTCGCTTTGAGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACAAGAGACGCAGAAACCA                                                       >  SRR4302536.1050067/1‑94 (MQ=60)

                                        ACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTG                                                      <  SRR4302536.623833/94‑1 (MQ=60)

                                        AACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTG                                                      <  SRR4302536.1999962/94‑1 (MQ=60)

                                         ACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGA                                                     <  SRR4302536.1264154/94‑1 (MQ=60)

                                          CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAC                                                    <  SRR4302536.3459709/94‑1 (MQ=60)

                                           GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACG                                                   >  SRR4302536.1119014/1‑94 (MQ=60)

                                           GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACG                                                   <  SRR4302536.2301163/94‑1 (MQ=60)

                                            CTCACGCTCGCTTTGCGGCGGTGGCAACAGCGAGGAAA‑TTTGGCGGCCGCTGTTGTTCACCACAAACAATACCAGCGGCGCAGTCACCTGACGC                                                  >  SRR4302536.1607818/1‑94 (MQ=53)

                                             TCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCA                                                 >  SRR4302536.5346301/1‑94 (MQ=60)

                                              CACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAA                                                >  SRR4302536.6278924/1‑94 (MQ=60)

                                               ACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAGTACCAGCGGGGCAGAAACCTGAGGCAAT                                               >  SRR4302536.5468191/1‑94 (MQ=60)

                                                CGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATA                                              <  SRR4302536.2883027/94‑1 (MQ=60)

                                                  CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAC                                            >  SRR4302536.1192646/1‑94 (MQ=60)

                                                  CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAC                                            >  SRR4302536.5406981/1‑94 (MQ=60)

                                                  CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAC                                            >  SRR4302536.5243236/1‑94 (MQ=60)

                                                  CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTAACCACAATTAATACCAGCGGCGACGAAACCTGACGCAATAAC                                            >  SRR4302536.3503679/1‑94 (MQ=60)

                                                  CTCGCTTGGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAC                                            <  SRR4302536.3648739/94‑1 (MQ=60)

                                                   TCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACG                                           <  SRR4302536.3198015/94‑1 (MQ=60)

                                                   TCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACG                                           <  SRR4302536.5162279/94‑1 (MQ=60)

                                                   CCGCTTGGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACG                                           <  SRR4302536.6442875/94‑1 (MQ=60)

                                                    CGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGC                                          <  SRR4302536.1369405/94‑1 (MQ=60)

                                                     GCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATCACGCC                                         >  SRR4302536.2005148/1‑94 (MQ=60)

                                                      CTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                        >  SRR4302536.2331003/1‑94 (MQ=60)

                                                       TTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCCCAATTAAAACCAGCGGCGCAGAAAaccgacgccaaaaagacaa                                       >  SRR4302536.3765400/1‑75 (MQ=60)

                                                        TTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGC                                      <  SRR4302536.6381001/94‑1 (MQ=60)

                                                            GGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGT                                  >  SRR4302536.1064579/1‑94 (MQ=60)

                                                             GCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTT                                 <  SRR4302536.867843/94‑1 (MQ=60)

                                                             GCGTGGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTT                                 <  SRR4302536.6918215/94‑1 (MQ=60)

                                                              CGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTG                                <  SRR4302536.459215/94‑1 (MQ=60)

                                                              CGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTG                                <  SRR4302536.2782890/94‑1 (MQ=60)

                                                               GTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA                               <  SRR4302536.5788260/94‑1 (MQ=60)

                                                               GTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA                               >  SRR4302536.5141548/1‑94 (MQ=60)

                                                               GTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA                               >  SRR4302536.4577975/1‑94 (MQ=60)

                                                                TTGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAG                              <  SRR4302536.4468316/94‑1 (MQ=60)

                                                                TGGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAG                              <  SRR4302536.5276873/94‑1 (MQ=60)

                                                                 TGGCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGA                             >  SRR4302536.3034240/1‑94 (MQ=60)

                                                                  GGCAACAGCGAGAAAA‑TTTCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                            <  SRR4302536.5911896/94‑1 (MQ=60)

                                                                   GCAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATC                           <  SRR4302536.2175216/94‑1 (MQ=60)

                                                                    CAACAGGGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCG                          <  SRR4302536.1785718/94‑1 (MQ=60)

                                                                    CAACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCG                          >  SRR4302536.5236648/1‑94 (MQ=60)

                                                                     AACAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGT                         >  SRR4302536.1558078/1‑94 (MQ=60)

                                                                       CAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                       >  SRR4302536.6517131/1‑94 (MQ=60)

                                                                       CAGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                       >  SRR4302536.2214385/1‑94 (MQ=60)

                                                                        AGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAA                      >  SRR4302536.1152503/1‑94 (MQ=60)

                                                                        AGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAA                      >  SRR4302536.6511375/1‑94 (MQ=60)

                                                                        AGCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAA                      <  SRR4302536.4526833/94‑1 (MQ=60)

                                                                         GCGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAG                     <  SRR4302536.2041225/94‑1 (MQ=60)

                                                                         GCGAGAAAA‑TTTCCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAG                     <  SRR4302536.3143468/94‑1 (MQ=60)

                                                                          CGAGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGT                    <  SRR4302536.5470119/94‑1 (MQ=60)

                                                                            AGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGC                  >  SRR4302536.6546369/1‑94 (MQ=60)

                                                                            AGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGC                  <  SRR4302536.3830916/94‑1 (MQ=60)

                                                                            AGAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGC                  <  SRR4302536.2056887/94‑1 (MQ=60)

                                                                             GAAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCG                 <  SRR4302536.2001471/94‑1 (MQ=60)

                                                                              AAAAGTTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCG                 <  SRR4302536.3990984/94‑1 (MQ=60)

                                                                              AAAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGG                >  SRR4302536.5704151/1‑94 (MQ=60)

                                                                               AAT‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGA               <  SRR4302536.1540920/94‑1 (MQ=60)

                                                                               AAA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGA               >  SRR4302536.3567035/1‑94 (MQ=60)

                                                                                AA‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAG              >  SRR4302536.5035544/1‑94 (MQ=60)

                                                                                 A‑TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTCACGCAATAACGCCAGCGCGCTGAGATCGTAAAGTGCGGAGA             >  SRR4302536.4652227/1‑94 (MQ=60)

                                                                                   TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAG            <  SRR4302536.1457873/94‑1 (MQ=60)

                                                                                   TTTGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAG            <  SRR4302536.835108/94‑1 (MQ=60)

                                                                                     TGCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAT          >  SRR4302536.6609588/1‑94 (MQ=60)

                                                                                      GCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC         >  SRR4302536.4624077/1‑94 (MQ=60)

                                                                                      GCCCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC         <  SRR4302536.4433627/94‑1 (MQ=60)

                                                                                        CCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGC       <  SRR4302536.5706111/94‑1 (MQ=60)

                                                                                        CCGCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGC       <  SRR4302536.2758658/94‑1 (MQ=60)

                                                                                          GCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC     >  SRR4302536.4688859/1‑94 (MQ=60)

                                                                                          GCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC     >  SRR4302536.3909938/1‑94 (MQ=60)

                                                                                          GCCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC     >  SRR4302536.928505/1‑94 (MQ=60)

                                                                                           CCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCA    <  SRR4302536.5705835/94‑1 (MQ=60)

                                                                                           CCGCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCA    <  SRR4302536.4523057/94‑1 (MQ=60)

                                                                                             GCTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACA  >  SRR4302536.3032601/1‑94 (MQ=60)

GCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAA‑TTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACA  >  NC_000913/2375778‑2375963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`