Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,483,814	C→T	H20Y (CAC→TAC)	crp →	cyclic AMP receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,483,814	0	C	T	90.7%	720.3 / 36.1	237	H20Y (CAC→TAC)	crp	cyclic AMP receptor protein
Reads supporting (aligned to +/- strand): ref base C (12/10); new base T (107/108); total (119/118)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 241 total aligned reads displayed.

TGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTG  >  NC_000913/3483725‑3483906

tGGAGAAAGCTTCTACCCGAGGATAACCGCGCATGGTGCTTCGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCtaca                                                                                           <  2:505807/93‑1 (MQ=255)

 ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCCTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAt                                                                                          >  2:623388/1‑93 (MQ=255)

  gagaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAtt                                                                                         <  1:2315502/93‑1 (MQ=255)

  gagaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAtt                                                                                         >  1:3234959/1‑93 (MQ=255)

   agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTc                                                                                        <  2:1860218/93‑1 (MQ=255)

    gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCa                                                                                       >  1:2407146/1‑93 (MQ=255)

     aaaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCAt                                                                                      <  1:158552/93‑1 (MQ=255)

      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCTACCTTCAtc                                                                                     >  1:3949387/1‑92 (MQ=255)

      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATa                                                                                     >  2:4547504/1‑93 (MQ=255)

      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATa                                                                                     <  2:3445539/93‑1 (MQ=255)

      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATa                                                                                     <  2:2917220/93‑1 (MQ=255)

       aGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATaa                                                                                    <  1:4031992/93‑1 (MQ=255)

        gCTTATAGCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   <  2:683257/93‑1 (MQ=255)

        gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   <  2:1241989/93‑1 (MQ=255)

        gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   >  1:544429/1‑93 (MQ=255)

        gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   >  2:3833075/1‑93 (MQ=255)

          ttATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTa                                                                                 >  1:1176577/1‑93 (MQ=255)

           tataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTAc                                                                                >  2:2393685/1‑93 (MQ=255)

           tataACAGAGGATAACCGCACATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTAc                                                                                >  2:2495468/1‑93 (MQ=255)

              aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCa                                                                             <  2:4363728/93‑1 (MQ=255)

              aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCa                                                                             <  2:4483823/93‑1 (MQ=255)

               aCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCAt                                                                            >  2:4657013/1‑93 (MQ=255)

                 cgcggATAACGGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATcc                                                                          <  1:43990/90‑1 (MQ=255)

                  gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCa                                                                         >  1:3825385/1‑93 (MQ=255)

                     gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAaga                                                                      >  2:1442740/1‑93 (MQ=255)

                      aTAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAagag                                                                     >  2:4563842/1‑93 (MQ=255)

                        aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCa                                                                   <  1:1013249/93‑1 (MQ=255)

                        aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCa                                                                   >  1:2973231/1‑93 (MQ=255)

                         aCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCAc                                                                  <  1:2730623/93‑1 (MQ=255)

                           cgcgCCTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGc                                                                <  1:321325/93‑1 (MQ=255)

                           cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGc                                                                >  1:2223593/1‑93 (MQ=255)

                           cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGc                                                                <  1:1018274/93‑1 (MQ=255)

                            gcgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCt                                                               >  1:4331587/1‑93 (MQ=255)

                              gcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTa                                                             <  2:4208618/93‑1 (MQ=255)

                              gcATGGTGCTTGGAAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCttt                                                             >  1:3694879/1‑92 (MQ=255)

                               cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTAt                                                            <  2:4147197/93‑1 (MQ=255)

                                 tGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTc                                                          <  2:3395664/93‑1 (MQ=255)

                                  ggTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCa                                                         >  1:1450290/1‑93 (MQ=255)

                                     gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa                                                      >  1:2148273/1‑93 (MQ=255)

                                     gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGGTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa                                                      >  1:1050165/1‑93 (MQ=255)

                                      cTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                     <  2:1035637/93‑1 (MQ=255)

                                       ttGTCAAACCGCAAACAGACCCGACTCTCGAATAGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                    <  1:4587643/93‑1 (MQ=255)

                                       ttGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                    <  2:3619886/93‑1 (MQ=255)

                                       ttGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                    <  2:3242608/93‑1 (MQ=255)

                                        tGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg                                                   <  1:4156533/93‑1 (MQ=255)

                                        tGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg                                                   <  1:888863/93‑1 (MQ=255)

                                         ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGt                                                  <  1:2047412/93‑1 (MQ=255)

                                          gCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTg                                                 <  2:3930445/93‑1 (MQ=255)

                                             aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                              >  1:3974097/1‑93 (MQ=255)

                                             aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                              <  1:2354833/93‑1 (MQ=255)

                                             aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                              >  2:2313984/1‑93 (MQ=255)

                                               ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                            <  2:2086805/93‑1 (MQ=255)

                                               ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                            <  2:2732829/93‑1 (MQ=255)

                                                cGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAg                                           <  2:2829105/93‑1 (MQ=255)

                                                   aaaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCgg                                        >  2:886004/1‑93 (MQ=255)

                                                    aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                       <  1:2551575/93‑1 (MQ=255)

                                                    aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                       >  1:2807288/1‑93 (MQ=255)

                                                     aCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                      <  1:1331271/93‑1 (MQ=255)

                                                      cggACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCGCGCTTATTCACCAGGGTGAAAAAGCGGaaa                                     <  1:2283368/91‑1 (MQ=255)

                                                      cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa                                     <  1:1980769/93‑1 (MQ=255)

                                                       aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                    <  1:290350/93‑1 (MQ=255)

                                                       aGACCCGACTCTCGAATGGATCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                    >  1:3703379/1‑93 (MQ=255)

                                                          cccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                 <  2:433216/93‑1 (MQ=255)

                                                          cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGAGAAAAAGCGGCAACGCt                                 >  2:4035139/1‑93 (MQ=255)

                                                           ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                >  2:4082860/1‑93 (MQ=255)

                                                           ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                <  2:736522/93‑1 (MQ=255)

                                                           ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                <  1:2605998/93‑1 (MQ=255)

                                                            cGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                               >  2:3903706/1‑93 (MQ=255)

                                                             gACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCATATTCACCAGGGTGAAAAAGCGGAAACGCTGta                              >  2:4391340/1‑93 (MQ=255)

                                                              aCTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtac                             >  2:3570610/1‑93 (MQ=255)

                                                                 ctcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtactac                          >  1:339525/1‑93 (MQ=255)

                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTCCa                         >  2:1584203/1‑93 (MQ=255)

                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         <  1:659671/93‑1 (MQ=255)

                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         <  2:3295798/93‑1 (MQ=255)

                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         >  2:3684583/1‑93 (MQ=255)

                                                                  tcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         >  1:1873773/1‑93 (MQ=255)

                                                                    gAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATc                       >  2:279047/1‑93 (MQ=255)

                                                                      aTGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                     <  1:2175265/93‑1 (MQ=255)

                                                                        ggTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                   <  2:2101890/93‑1 (MQ=255)

                                                                          ttCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa                 <  2:101242/93‑1 (MQ=255)

                                                                           tCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGCAAAAGCGGCAACGCTGTACTACATCGTTAAAg                >  2:2421870/1‑93 (MQ=255)

                                                                             ttGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc              >  1:3254558/1‑93 (MQ=255)

                                                                             ttGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc              <  2:2587283/93‑1 (MQ=255)

                                                                               gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc            <  1:1226374/93‑1 (MQ=255)

                                                                               gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc            >  1:3001272/1‑93 (MQ=255)

                                                                               gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGCGAAAAAGCGGAACCGCTGTACTACATCGTTAAAGActc            >  2:3713463/1‑93 (MQ=255)

                                                                               gTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc            >  2:3583988/1‑93 (MQ=255)

                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           <  1:1427610/93‑1 (MQ=255)

                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           >  2:4173876/1‑93 (MQ=255)

                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           >  2:613318/1‑93 (MQ=255)

                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           >  1:4107757/1‑93 (MQ=255)

                                                                                 ctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtg          >  1:170473/1‑93 (MQ=255)

                                                                                 ctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtg          <  2:984431/93‑1 (MQ=255)

                                                                                  tcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt         >  2:2288518/1‑93 (MQ=255)

                                                                                  tcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt         <  1:721199/93‑1 (MQ=255)

                                                                                   cATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg        <  2:1313562/93‑1 (MQ=255)

                                                                                   cATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg        <  2:1204595/93‑1 (MQ=255)

                                                                                   cATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGGGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg        >  1:3560985/1‑93 (MQ=255)

                                                                                     ttGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc      >  2:3020139/1‑93 (MQ=255)

                                                                                         cacaTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg  <  2:3165709/93‑1 (MQ=255)

TGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTG  >  NC_000913/3483725‑3483906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTG  >  NC_000913/3483721‑3483906

GCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCT                                                                                              <  SRR4302544.1456785/94‑1 (MQ=60)

 CTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTA                                                                                             >  SRR4302544.2002723/1‑94 (MQ=60)

  TCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTAC                                                                                            <  SRR4302544.712244/94‑1 (MQ=60)

    TGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAT                                                                                          >  SRR4302544.1477379/1‑94 (MQ=60)

    TGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACAT                                                                                          >  SRR4302544.2093833/1‑94 (MQ=60)

     GGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATT                                                                                         >  SRR4302544.3265926/1‑94 (MQ=60)

      GAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTC                                                                                        <  SRR4302544.2337417/94‑1 (MQ=60)

       AGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCA                                                                                       >  SRR4302544.2430009/1‑94 (MQ=60)

         AAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATA                                                                                     >  SRR4302544.3988635/1‑94 (MQ=60)

         AAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATA                                                                                     <  SRR4302544.160103/94‑1 (MQ=60)

         AAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGCTCTTGTCTCATTGCTACATTCATA                                                                                     >  SRR4302544.204499/1‑94 (MQ=60)

           AGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAG                                                                                   <  SRR4302544.4072155/94‑1 (MQ=60)

           AGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAG                                                                                   >  SRR4302544.549782/1‑94 (MQ=60)

             CTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTC                                                                                 >  SRR4302544.4426741/1‑94 (MQ=60)

             CTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTA                                                                                 >  SRR4302544.1188304/1‑94 (MQ=60)

                 TAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCA                                                                             >  SRR4302544.2229838/1‑94 (MQ=60)

                  AACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCAT                                                                            >  SRR4302544.2661109/1‑94 (MQ=60)

                   ACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATC                                                                           >  SRR4302544.3153757/1‑94 (MQ=60)

                     cgcGGATAACGGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCA                                                                         <  SRR4302544.44421/91‑1 (MQ=60)

                     AGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCA                                                                         >  SRR4302544.3863319/1‑94 (MQ=60)

                      GAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAA                                                                        >  SRR4302544.11959/1‑94 (MQ=60)

                        GGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGA                                                                      >  SRR4302544.2580882/1‑94 (MQ=60)

                         GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAG                                                                     <  SRR4302544.1120009/94‑1 (MQ=60)

                          ATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGC                                                                    >  SRR4302544.3117254/1‑94 (MQ=60)

                           TAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCA                                                                   >  SRR4302544.3001747/1‑94 (MQ=60)

                            AACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCAC                                                                  <  SRR4302544.1023347/94‑1 (MQ=60)

                            AACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCAC                                                                  <  SRR4302544.1851387/94‑1 (MQ=60)

                             ACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACG                                                                 <  SRR4302544.2756941/94‑1 (MQ=60)

                              CCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGC                                                                >  SRR4302544.2244514/1‑94 (MQ=60)

                               CGCGCCTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCT                                                               <  SRR4302544.324665/94‑1 (MQ=60)

                               CGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCT                                                               <  SRR4302544.2674014/94‑1 (MQ=60)

                               CGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCT                                                               <  SRR4302544.324666/94‑1 (MQ=60)

                               CGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCT                                                               >  SRR4302544.4375689/1‑94 (MQ=60)

                               CGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCT                                                               <  SRR4302544.1028439/94‑1 (MQ=60)

                               CGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCT                                                               <  SRR4302544.1028440/94‑1 (MQ=60)

                                GCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTT                                                              >  SRR4302544.3902572/1‑94 (MQ=60)

                                 CGCATGGTGCTTGGAAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTT                                                             >  SRR4302544.3731685/1‑94 (MQ=60)

                                   CATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATT                                                           <  SRR4302544.2512189/94‑1 (MQ=60)

                                   CATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATT                                                           <  SRR4302544.2512188/94‑1 (MQ=60)

                                    ATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTC                                                          <  SRR4302544.1247283/94‑1 (MQ=60)

                                     TGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCA                                                         >  SRR4302544.1464702/1‑94 (MQ=60)

                                      GGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAC                                                        >  SRR4302544.2240698/1‑94 (MQ=60)

                                        TGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCA                                                      >  SRR4302544.2168731/1‑94 (MQ=60)

                                        TGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGGTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCA                                                      >  SRR4302544.1060569/1‑94 (MQ=60)

                                          CTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGG                                                    >  SRR4302544.454212/1‑94 (MQ=60)

                                          CTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGG                                                    <  SRR4302544.617826/94‑1 (MQ=60)

                                           TTGTCAAACCGCAAACAGACCCGACTCTCGAATAGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGG                                                   <  SRR4302544.4633308/94‑1 (MQ=60)

                                            TGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGT                                                  <  SRR4302544.4197702/94‑1 (MQ=60)

                                            TGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGT                                                  <  SRR4302544.1738189/94‑1 (MQ=60)

                                            TGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGT                                                  <  SRR4302544.897672/94‑1 (MQ=60)

                                             GGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTG                                                 <  SRR4302544.599876/94‑1 (MQ=60)

                                             GGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTG                                                 <  SRR4302544.2067141/94‑1 (MQ=60)

                                               CAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAA                                               <  SRR4302544.1713145/94‑1 (MQ=60)

                                                AAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAA                                              >  SRR4302544.4013641/1‑94 (MQ=60)

                                                 AACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAA                                             <  SRR4302544.2377142/94‑1 (MQ=60)

                                                  ACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAA                                            <  SRR4302544.371824/94‑1 (MQ=60)

                                                   CCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAG                                           <  SRR4302544.968541/94‑1 (MQ=60)

                                                     GCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAGCAGGGTGAAAAAGCG                                         <  SRR4302544.2180055/94‑1 (MQ=60)

                                                     CCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCG                                         <  SRR4302544.2429109/94‑1 (MQ=60)

                                                       AAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGA                                       >  SRR4302544.2834365/1‑94 (MQ=60)

                                                       AAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGA                                       >  SRR4302544.2867117/1‑94 (MQ=60)

                                                        AACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAA                                      <  SRR4302544.2576070/94‑1 (MQ=60)

                                                         ACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAA                                     >  SRR4302544.1917474/1‑94 (MQ=60)

                                                         ACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAA                                     <  SRR4302544.1344370/94‑1 (MQ=60)

                                                          CGGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCGCGCTTATTCACCAGGGTGAAAAAGCGGAAAC                                    <  SRR4302544.2304885/94‑1 (MQ=60)

                                                          CAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAC                                    <  SRR4302544.2356969/94‑1 (MQ=60)

                                                          CAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAC                                    <  SRR4302544.2000011/94‑1 (MQ=60)

                                                          CAGACCCGACTCTCGAATGGATCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAC                                    >  SRR4302544.3740237/1‑94 (MQ=60)

                                                           AGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACG                                   <  SRR4302544.293354/94‑1 (MQ=60)

                                                            GACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGC                                  >  SRR4302544.3020191/1‑94 (MQ=60)

                                                               CCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGT                               <  SRR4302544.2631029/94‑1 (MQ=60)

                                                                CGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTA                              <  SRR4302544.4116129/94‑1 (MQ=60)

                                                                CGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTA                              >  SRR4302544.3735803/1‑94 (MQ=60)

                                                                  ACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACT                            <  SRR4302544.426219/94‑1 (MQ=60)

                                                                  ACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACT                            <  SRR4302544.3854024/94‑1 (MQ=60)

                                                                    TCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTAC                          >  SRR4302544.343041/1‑94 (MQ=60)

                                                                     CTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACA                         <  SRR4302544.418221/94‑1 (MQ=60)

                                                                     CTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACA                         >  SRR4302544.1891882/1‑94 (MQ=60)

                                                                      TCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAT                        <  SRR4302544.666194/94‑1 (MQ=60)

                                                                       CGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATC                       >  SRR4302544.4747691/1‑94 (MQ=60)

                                                                       CGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATC                       >  SRR4302544.1033337/1‑94 (MQ=60)

                                                                         AATGGTTCTTGTCTCATTGCTTCATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAGAAGCGGAAACGCTGTACTACCTCGT                     >  SRR4302544.3397064/1‑94 (MQ=60)

                                                                         AATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGT                     >  SRR4302544.958034/1‑94 (MQ=60)

                                                                          ATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTT                    <  SRR4302544.2771786/94‑1 (MQ=60)

                                                                          ATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTT                    <  SRR4302544.2195875/94‑1 (MQ=60)

                                                                          ATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTT                    <  SRR4302544.1118338/94‑1 (MQ=60)

                                                                          ATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCAAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACaaaatagta                    >  SRR4302544.1692617/1‑85 (MQ=60)

                                                                           TGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTA                   >  SRR4302544.713688/1‑94 (MQ=60)

                                                                            GGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAA                  >  SRR4302544.795797/1‑94 (MQ=60)

                                                                             GTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA                 >  SRR4302544.4495612/1‑94 (MQ=60)

                                                                             GTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA                 <  SRR4302544.4055211/94‑1 (MQ=60)

                                                                              TTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAG                <  SRR4302544.216290/94‑1 (MQ=60)

                                                                              TTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAG                <  SRR4302544.3441544/94‑1 (MQ=60)

                                                                              GTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAG                <  SRR4302544.1284185/94‑1 (MQ=60)

                                                                               TCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG               <  SRR4302544.3403945/94‑1 (MQ=60)

                                                                                CTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGC              >  SRR4302544.3285681/1‑94 (MQ=60)

                                                                                CTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGC              >  SRR4302544.4051700/1‑94 (MQ=60)

                                                                                CTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGC              >  SRR4302544.3285679/1‑94 (MQ=60)

                                                                                 TTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCT             <  SRR4302544.2955986/94‑1 (MQ=60)

                                                                                 TTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCT             <  SRR4302544.2954218/94‑1 (MQ=60)

                                                                                 TTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCT             <  SRR4302544.2372535/94‑1 (MQ=60)

                                                                                  TGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTC            >  SRR4302544.3030045/1‑94 (MQ=60)

                                                                                   GTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCT           <  SRR4302544.1238504/94‑1 (MQ=60)

                                                                                   GTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCT           <  SRR4302544.2244898/94‑1 (MQ=60)

                                                                                   GTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCT           >  SRR4302544.4148596/1‑94 (MQ=60)

                                                                                    TCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTG          <  SRR4302544.1441812/94‑1 (MQ=60)

                                                                                    TCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTG          <  SRR4302544.1441811/94‑1 (MQ=60)

                                                                                    TCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTG          <  SRR4302544.1402862/94‑1 (MQ=60)

                                                                                    TCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTG          >  SRR4302544.172153/1‑94 (MQ=60)

                                                                                    TCTCATTGCCACATTCATAAGTACTCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTG          <  SRR4302544.3702959/94‑1 (MQ=60)

                                                                                     CTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGT         >  SRR4302544.1498849/1‑94 (MQ=60)

                                                                                      TCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTG        <  SRR4302544.728307/94‑1 (MQ=60)

                                                                                      TCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGGGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTG        >  SRR4302544.3595123/1‑94 (MQ=60)

                                                                                       CATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGG       >  SRR4302544.4316398/1‑94 (MQ=60)

                                                                                        ATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGC      <  SRR4302544.4013446/94‑1 (MQ=60)

                                                                                         TTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTTGCA     >  SRR4302544.2335154/1‑94 (MQ=60)

                                                                                           GCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGT   <  SRR4302544.2350741/94‑1 (MQ=60)

                                                                                           GCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGT   >  SRR4302544.1158998/1‑94 (MQ=60)

                                                                                           GCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGT   <  SRR4302544.3873669/94‑1 (MQ=60)

                                                                                           GCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGT   <  SRR4302544.3438551/94‑1 (MQ=60)

                                                                                            CTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTG  <  SRR4302544.897555/94‑1 (MQ=60)

GCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTG  >  NC_000913/3483721‑3483906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`