Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,484,054 T→C Y100H (TAC→CAC)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,484,0540TC98.6% 668.1 / ‑1.4 209Y100H (TAC→CAC) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (91/115);  total (91/118)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 216 total aligned reads displayed.

TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143
                                                                                          |                                                                                         
tttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAc                                                                                         <  1:2428581/93‑1 (MQ=255)
tttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAc                                                                                         >  1:1114373/1‑93 (MQ=255)
 ttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGcaca                                                                                        <  1:1001723/93‑1 (MQ=255)
  tATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaa                                                                                       >  2:493081/1‑93 (MQ=255)
    ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaa                                                                                     >  2:1670568/1‑93 (MQ=255)
      ggCGAACTGCACCTGTTTGAAGAGGTCCATGACCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaaaa                                                                                   <  2:2671653/93‑1 (MQ=255)
        cGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAtt                                                                                 >  2:3916571/1‑93 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                >  1:554407/1‑93 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                >  2:4581142/1‑93 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                >  1:1624061/1‑93 (MQ=255)
             tGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            >  1:2888393/1‑93 (MQ=255)
               ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCaa                                                                          <  1:4391340/93‑1 (MQ=255)
                gCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAt                                                                         <  2:3491398/93‑1 (MQ=255)
                  cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTg                                                                       >  2:2005856/1‑93 (MQ=255)
                     tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAtt                                                                    <  2:1158602/93‑1 (MQ=255)
                      tttaaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTc                                                                   >  1:2007413/4‑93 (MQ=255)
                      ttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTc                                                                   <  1:3233765/93‑1 (MQ=255)
                        gAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAg                                                                 <  2:3254558/93‑1 (MQ=255)
                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                <  1:2663519/93‑1 (MQ=255)
                          agagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGt                                                               <  1:2306768/93‑1 (MQ=255)
                          agagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGt                                                               <  1:4142371/93‑1 (MQ=255)
                          agagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGt                                                               <  1:4657013/93‑1 (MQ=255)
                           gagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTa                                                              >  2:1039323/1‑93 (MQ=255)
                           gagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTa                                                              <  2:2148273/93‑1 (MQ=255)
                            agGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTaa                                                             >  1:1177922/1‑93 (MQ=255)
                             gggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                            >  1:1897426/1‑93 (MQ=255)
                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  1:3992504/93‑1 (MQ=255)
                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  2:713426/93‑1 (MQ=255)
                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  1:3631359/93‑1 (MQ=255)
                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                         >  2:132769/1‑93 (MQ=255)
                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                         >  1:1015477/1‑93 (MQ=255)
                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                         <  1:1725243/93‑1 (MQ=255)
                                   ggAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGa                                                      >  1:1052786/1‑93 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     >  2:3728836/1‑93 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  1:2098007/93‑1 (MQ=255)
                                     aaCGTAGCGAATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                    >  1:1560809/1‑93 (MQ=255)
                                      aCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                   <  1:3574198/93‑1 (MQ=255)
                                      aCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                   <  1:2753376/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAACCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  2:2630124/93‑1 (MQ=255)
                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                <  1:2240456/93‑1 (MQ=255)
                                           gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                              <  1:2511787/93‑1 (MQ=255)
                                           gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGGAAACCCGGACATTCTGa                                              >  2:793985/1‑93 (MQ=255)
                                             gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                            <  1:182718/93‑1 (MQ=255)
                                             gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                            >  2:2853932/1‑93 (MQ=255)
                                              cATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                           <  1:4260545/93‑1 (MQ=255)
                                               aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCg                                          >  2:4234202/1‑93 (MQ=255)
                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  1:623294/93‑1 (MQ=255)
                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         >  1:3167959/1‑93 (MQ=255)
                                                 gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        <  1:4547406/93‑1 (MQ=255)
                                                 gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        <  1:1097002/93‑1 (MQ=255)
                                                 gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        >  1:4360552/1‑93 (MQ=255)
                                                  ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       <  1:4324248/93‑1 (MQ=255)
                                                   gTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTg                                      <  2:4500518/93‑1 (MQ=255)
                                                    gaCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                     <  2:229188/92‑1 (MQ=255)
                                                     aCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                    <  1:4173876/93‑1 (MQ=255)
                                                      cGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCAAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCt                                   <  2:3741701/93‑1 (MQ=255)
                                                      agggCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCt                                   <  1:2313984/90‑1 (MQ=255)
                                                       gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                  >  1:2975407/1‑93 (MQ=255)
                                                        tGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                 >  2:2381618/1‑93 (MQ=255)
                                                        tGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                 >  2:332154/1‑93 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              >  2:1253173/1‑93 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              <  2:3124061/93‑1 (MQ=255)
                                                             aacccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  1:989204/90‑1 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  2:3876219/93‑1 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  1:647097/93‑1 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  1:949194/93‑1 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           <  1:68501/93‑1 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           >  1:3307511/1‑93 (MQ=255)
                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          >  2:3135709/1‑93 (MQ=255)
                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          <  1:4587403/93‑1 (MQ=255)
                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACCGATg                          >  2:2299172/1‑93 (MQ=255)
                                                                ccGCTTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         <  2:3014904/93‑1 (MQ=255)
                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         <  1:3684583/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  1:4472452/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  2:4382354/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  2:4272777/93‑1 (MQ=255)
                                                                  gCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                       >  1:1713963/1‑93 (MQ=255)
                                                                  gCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                       >  1:2234310/1‑93 (MQ=255)
                                                                   ccTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcgc                      >  1:2955424/1‑93 (MQ=255)
                                                                    cTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                     >  1:3583449/1‑93 (MQ=255)
                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    <  2:614122/93‑1 (MQ=255)
                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    >  1:1214616/1‑93 (MQ=255)
                                                                      gtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                   <  2:1940912/93‑1 (MQ=255)
                                                                      gtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                   >  1:4040450/1‑93 (MQ=255)
                                                                        gAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcgt                 >  2:1134717/1‑93 (MQ=255)
                                                                         aaGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                <  1:3020714/93‑1 (MQ=255)
                                                                         aaGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                >  1:1311440/1‑93 (MQ=255)
                                                                         aaGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                <  1:343719/93‑1 (MQ=255)
                                                                          aGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt               <  2:3164539/93‑1 (MQ=255)
                                                                             ggCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa            <  2:3364944/93‑1 (MQ=255)
                                                                             ggCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa            >  1:1380330/1‑93 (MQ=255)
                                                                               cTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAg          >  1:77478/1‑93 (MQ=255)
                                                                                tGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt         <  1:613318/93‑1 (MQ=255)
                                                                                tGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt         >  2:109024/1‑93 (MQ=255)
                                                                                  aaaTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa       >  2:2746600/1‑93 (MQ=255)
                                                                                  aaaTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa       >  2:1542828/1‑93 (MQ=255)
                                                                                  aaaTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa       >  2:907950/1‑93 (MQ=255)
                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     <  2:1884069/93‑1 (MQ=255)
                                                                                      ttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTc   <  2:4107930/93‑1 (MQ=255)
                                                                                       tCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCCCTTCa  <  2:860555/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483961‑3484147
                                                                                             |                                                                                             
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGC                                                                                               >  SRR4302544.1625805/1‑94 (MQ=60)
  TTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAC                                                                                             <  SRR4302544.4144989/94‑1 (MQ=60)
  TTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAC                                                                                             >  SRR4302544.1125491/1‑94 (MQ=60)
   TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACA                                                                                            <  SRR4302544.2451698/94‑1 (MQ=60)
    TTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAA                                                                                           <  SRR4302544.1011658/94‑1 (MQ=60)
    TTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAA                                                                                           <  SRR4302544.1011657/94‑1 (MQ=60)
         GGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGTAATTTCGCACAAAAAAT                                                                                      <  SRR4302544.2310091/94‑1 (MQ=60)
         GGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAT                                                                                      <  SRR4302544.1593202/94‑1 (MQ=60)
          GCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATT                                                                                     <  SRR4302544.4609348/94‑1 (MQ=60)
           CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    >  SRR4302544.559829/1‑94 (MQ=60)
           CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    >  SRR4302544.1640064/1‑94 (MQ=60)
             AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  >  SRR4302544.442175/1‑94 (MQ=60)
             AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  <  SRR4302544.894777/94‑1 (MQ=60)
               CTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCC                                                                                >  SRR4302544.597942/1‑94 (MQ=60)
               CTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCC                                                                                >  SRR4302544.2916238/1‑94 (MQ=60)
               CTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCC                                                                                >  SRR4302544.2916239/1‑94 (MQ=60)
                 GGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTATGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCTA                                                                              >  SRR4302544.3720547/1‑94 (MQ=60)
                  GGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAT                                                                             <  SRR4302544.4435769/94‑1 (MQ=60)
                   GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            <  SRR4302544.1198960/94‑1 (MQ=60)
                    CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           <  SRR4302544.4343650/94‑1 (MQ=60)
                    CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           <  SRR4302544.1094363/94‑1 (MQ=60)
                    CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           >  SRR4302544.339058/1‑94 (MQ=60)
                     CTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGA                                                                          <  SRR4302544.1659699/94‑1 (MQ=60)
                     CTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGA                                                                          >  SRR4302544.4297271/1‑94 (MQ=60)
                      TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAT                                                                         >  SRR4302544.193936/1‑94 (MQ=60)
                       GTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATT                                                                        >  SRR4302544.1798209/1‑94 (MQ=60)
                        TTTTAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTC                                                                       >  SRR4302544.2026921/1‑94 (MQ=60)
                        TTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTC                                                                       <  SRR4302544.1043810/94‑1 (MQ=60)
                         TTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCA                                                                      <  SRR4302544.3264717/94‑1 (MQ=60)
                           GAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGG                                                                    <  SRR4302544.1893877/94‑1 (MQ=60)
                            AAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGT                                                                   <  SRR4302544.2689053/94‑1 (MQ=60)
                            AAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGT                                                                   <  SRR4302544.2689054/94‑1 (MQ=60)
                             AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  <  SRR4302544.4183377/94‑1 (MQ=60)
                             AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  <  SRR4302544.2328567/94‑1 (MQ=60)
                             AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  <  SRR4302544.1355910/94‑1 (MQ=60)
                             AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  <  SRR4302544.4703091/94‑1 (MQ=60)
                              GAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAA                                                                 >  SRR4302544.1189661/1‑94 (MQ=60)
                               AGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAA                                                                >  SRR4302544.1915767/1‑94 (MQ=60)
                                 GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              <  SRR4302544.3667540/94‑1 (MQ=60)
                                 GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              <  SRR4302544.4032245/94‑1 (MQ=60)
                                  GCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCC                                                             >  SRR4302544.1025606/1‑94 (MQ=60)
                                   CCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCG                                                            <  SRR4302544.1300863/94‑1 (MQ=60)
                                   CCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCG                                                            <  SRR4302544.1742174/94‑1 (MQ=60)
                                    CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGG                                                           <  SRR4302544.63069/94‑1 (MQ=60)
                                     AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302544.3567125/1‑94 (MQ=60)
                                     AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302544.4668748/1‑94 (MQ=60)
                                     AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302544.1063227/1‑94 (MQ=60)
                                     AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302544.1739398/1‑94 (MQ=60)
                                      GGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAC                                                         <  SRR4302544.1759682/94‑1 (MQ=60)
                                       GAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACA                                                        <  SRR4302544.2118193/94‑1 (MQ=60)
                                       GAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACA                                                        <  SRR4302544.2099756/94‑1 (MQ=60)
                                       GAACGTAGCGAATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACA                                                        >  SRR4302544.1576313/1‑94 (MQ=60)
                                         ACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATT                                                      <  SRR4302544.3608475/94‑1 (MQ=60)
                                         ACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATT                                                      <  SRR4302544.2779884/94‑1 (MQ=60)
                                           GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    >  SRR4302544.3650364/1‑94 (MQ=60)
                                            TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   <  SRR4302544.3297632/94‑1 (MQ=60)
                                            TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   <  SRR4302544.2261535/94‑1 (MQ=60)
                                              GCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAT                                                 <  SRR4302544.4416642/94‑1 (MQ=60)
                                              GCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAT                                                 <  SRR4302544.2535893/94‑1 (MQ=60)
                                               CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                <  SRR4302544.4392484/94‑1 (MQ=60)
                                                GCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGC                                               <  SRR4302544.184525/94‑1 (MQ=60)
                                                 CATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCG                                              >  SRR4302544.2988234/1‑94 (MQ=60)
                                                 CATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCG                                              <  SRR4302544.4303995/94‑1 (MQ=60)
                                                  ATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGT                                             >  SRR4302544.3198108/1‑94 (MQ=60)
                                                   TGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            >  SRR4302544.4404802/1‑94 (MQ=60)
                                                   TGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            <  SRR4302544.629414/94‑1 (MQ=60)
                                                    GGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTT                                           <  SRR4302544.1107912/94‑1 (MQ=60)
                                                    GGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTT                                           <  SRR4302544.4592821/94‑1 (MQ=60)
                                                     GGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          <  SRR4302544.4074663/94‑1 (MQ=60)
                                                     GGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          >  SRR4302544.453827/1‑94 (MQ=60)
                                                     GGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          <  SRR4302544.4368285/94‑1 (MQ=60)
                                                      GTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGT                                         >  SRR4302544.1258681/1‑94 (MQ=60)
                                                        ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302544.4215217/94‑1 (MQ=60)
                                                        ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302544.4215218/94‑1 (MQ=60)
                                                        ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302544.1427557/94‑1 (MQ=60)
                                                         aggGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTG                                      <  SRR4302544.2335880/91‑1 (MQ=60)
                                                         CGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTG                                      >  SRR4302544.2144984/1‑94 (MQ=60)
                                                         CGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTG                                      >  SRR4302544.3003948/1‑94 (MQ=60)
                                                            GCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCAC                                   <  SRR4302544.4075330/94‑1 (MQ=60)
                                                            GCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCCGACATTCTGATGCGTTTGTCTGCAC                                   <  SRR4302544.653971/94‑1 (MQ=60)
                                                             CGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACA                                  >  SRR4302544.287846/1‑94 (MQ=60)
                                                               AAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGA                                >  SRR4302544.1135626/1‑94 (MQ=60)
                                                                AACCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302544.998932/94‑1 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302544.958532/94‑1 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302544.653486/94‑1 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               >  SRR4302544.3339008/1‑94 (MQ=60)
                                                                 AACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATG                              <  SRR4302544.69176/94‑1 (MQ=60)
                                                                  ACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGG                             <  SRR4302544.4633067/94‑1 (MQ=60)
                                                                   CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302544.3721320/94‑1 (MQ=60)
                                                                    CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           <  SRR4302544.111431/94‑1 (MQ=60)
                                                                    CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           >  SRR4302544.1730822/1‑94 (MQ=60)
                                                                    CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           >  SRR4302544.2255327/1‑94 (MQ=60)
                                                                    CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           >  SRR4302544.2255328/1‑94 (MQ=60)
                                                                    CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           <  SRR4302544.4517432/94‑1 (MQ=60)
                                                                     GCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGC                          <  SRR4302544.30583/94‑1 (MQ=60)
                                                                     GCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGC                          >  SRR4302544.2983806/1‑94 (MQ=60)
                                                                      CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302544.2330052/1‑94 (MQ=60)
                                                                      CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302544.971981/1‑94 (MQ=60)
                                                                      CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302544.3617836/1‑94 (MQ=60)
                                                                      CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302544.3617837/1‑94 (MQ=60)
                                                                       CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGCCATTCTGATGCGTTTGTCTGCCACGATGGCGCGT                        >  SRR4302544.3459607/1‑94 (MQ=60)
                                                                       CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        >  SRR4302544.1226639/1‑94 (MQ=60)
                                                                       CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        <  SRR4302544.3217602/94‑1 (MQ=60)
                                                                        TGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTC                       >  SRR4302544.4080685/1‑94 (MQ=60)
                                                                           GAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTC                    >  SRR4302544.1324338/1‑94 (MQ=60)
                                                                            AAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCT                   <  SRR4302544.3049665/94‑1 (MQ=60)
                                                                            AAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCT                   <  SRR4302544.347265/94‑1 (MQ=60)
                                                                              GTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGC                 >  SRR4302544.3006478/1‑94 (MQ=60)
                                                                               TGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCA                >  SRR4302544.1394111/1‑94 (MQ=60)
                                                                               GGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCA                <  SRR4302544.578497/94‑1 (MQ=60)
                                                                                GGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAA               >  SRR4302544.240855/1‑94 (MQ=60)
                                                                                 GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              >  SRR4302544.78228/1‑94 (MQ=60)
                                                                                  CTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGT             >  SRR4302544.2473579/1‑94 (MQ=60)
                                                                                   TGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            <  SRR4302544.619334/94‑1 (MQ=60)
                                                                                   TGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            <  SRR4302544.619335/94‑1 (MQ=60)
                                                                                    GAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCA           <  SRR4302544.4488970/94‑1 (MQ=60)
                                                                                      AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         <  SRR4302544.3764580/94‑1 (MQ=60)
                                                                                      AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         >  SRR4302544.2908234/1‑94 (MQ=60)
                                                                                      AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         >  SRR4302544.2307508/1‑94 (MQ=60)
                                                                                       ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        >  SRR4302544.549942/1‑94 (MQ=60)
                                                                                       ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        >  SRR4302544.2659982/1‑94 (MQ=60)
                                                                                        TTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTC       >  SRR4302544.4137127/1‑94 (MQ=60)
                                                                                        TTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTC       <  SRR4302544.735023/94‑1 (MQ=60)
                                                                                          TCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAG     >  SRR4302544.433601/1‑94 (MQ=60)
                                                                                           CGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGA    >  SRR4302544.2190439/1‑94 (MQ=60)
                                                                                           CGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGA    >  SRR4302544.852700/1‑94 (MQ=60)
                                                                                           CGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGA    >  SRR4302544.4207399/1‑94 (MQ=60)
                                                                                            GCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAG   <  SRR4302544.1411131/94‑1 (MQ=60)
                                                                                            GCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAG   >  SRR4302544.165039/1‑94 (MQ=60)
                                                                                             CACAAAAAATTTCTCCAATTGATTCATGTAAAGCCGGACATTCTGAGGCGTTTGTCTGCACAGATGTCGCGTCGTCGGCAAGTCACTTCAGAGA  >  SRR4302544.4672326/1‑94 (MQ=60)
                                                                                             CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  SRR4302544.3229775/1‑94 (MQ=60)
                                                                                             CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  <  SRR4302544.239098/94‑1 (MQ=60)
                                                                                             |                                                                                             
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483961‑3484147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑