Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,484,054 T→C Y100H (TAC→CAC)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,484,0540TC100.0% 878.5 / NA 268Y100H (TAC→CAC) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (121/147);  total (121/147)

Only 100 of 273 total aligned reads displayed.

TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143
                                                                                          |                                                                                         
   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      >  2:4756941/1‑93 (MQ=255)
   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      <  2:4683282/93‑1 (MQ=255)
    ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaa                                                                                     <  1:2760786/93‑1 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                <  1:5579913/93‑1 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                >  2:3085171/1‑93 (MQ=255)
          aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTc                                                                               <  1:349372/93‑1 (MQ=255)
          aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTc                                                                               <  1:2661653/93‑1 (MQ=255)
            cTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGc                                                                             <  2:5804609/93‑1 (MQ=255)
             tGGTCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            <  1:5172928/93‑1 (MQ=255)
             tGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            >  2:147476/1‑93 (MQ=255)
             tGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            <  1:4859521/93‑1 (MQ=255)
              gggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCa                                                                           <  2:2297869/93‑1 (MQ=255)
               ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCaa                                                                          <  2:2589539/93‑1 (MQ=255)
               ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCaa                                                                          >  2:5422763/1‑93 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  2:6657573/93‑1 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  1:3374705/93‑1 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  2:4516085/93‑1 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGCACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        >  2:3917391/1‑93 (MQ=255)
                  cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTg                                                                       <  2:2966277/93‑1 (MQ=255)
                  cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTg                                                                       >  2:1372712/1‑93 (MQ=255)
                   tGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAttca                                                                      <  1:6617708/93‑3 (MQ=255)
                   tGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGa                                                                      <  1:4365041/93‑1 (MQ=255)
                    gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAt                                                                     >  2:6329419/1‑93 (MQ=255)
                     tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAtt                                                                    <  1:4201653/93‑1 (MQ=255)
                       tGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCa                                                                  <  1:2178146/93‑1 (MQ=255)
                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                >  2:6570893/1‑93 (MQ=255)
                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                <  2:2499542/93‑1 (MQ=255)
                            agGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTaa                                                             >  2:4299492/1‑93 (MQ=255)
                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  1:3229999/93‑1 (MQ=255)
                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                         >  2:32801/1‑93 (MQ=255)
                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        >  1:4524529/1‑93 (MQ=255)
                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        <  1:2820332/93‑1 (MQ=255)
                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        <  2:6461773/93‑1 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAGCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  2:5580388/93‑1 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  1:3966677/93‑1 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  1:78219/93‑1 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  1:5259550/93‑1 (MQ=255)
                                     aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                    <  2:1635291/93‑1 (MQ=255)
                                     aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                    <  1:4671374/93‑1 (MQ=255)
                                      aCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                   >  2:112134/1‑93 (MQ=255)
                                       cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                  <  2:5857236/93‑1 (MQ=255)
                                       cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                  <  2:287038/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  2:865005/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  1:2104753/93‑1 (MQ=255)
                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                >  2:6094085/1‑93 (MQ=255)
                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                <  1:1316309/93‑1 (MQ=255)
                                           gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                              <  2:2445715/93‑1 (MQ=255)
                                             gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCCGATg                                            <  2:5097410/93‑1 (MQ=255)
                                              cATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                           >  1:4961386/1‑93 (MQ=255)
                                               aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCg                                          <  1:3033213/93‑1 (MQ=255)
                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  1:1027015/93‑1 (MQ=255)
                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  1:5868874/93‑1 (MQ=255)
                                                agggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  1:6395944/92‑1 (MQ=255)
                                                 gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        >  1:1830297/1‑93 (MQ=255)
                                                 gggTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        <  1:115836/93‑1 (MQ=255)
                                                  ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       >  2:3493481/1‑93 (MQ=255)
                                                     aCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                    <  1:2459363/93‑1 (MQ=255)
                                                       gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                  >  2:1497114/1‑93 (MQ=255)
                                                       gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                  <  1:6000612/93‑1 (MQ=255)
                                                        tGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                 >  1:4468896/1‑93 (MQ=255)
                                                        tGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                 <  1:6478910/93‑1 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              >  1:6560695/1‑93 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              <  1:1488301/93‑1 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           >  1:1687228/1‑93 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           >  2:3320027/1‑93 (MQ=255)
                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          <  1:251504/93‑1 (MQ=255)
                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          >  2:797310/1‑93 (MQ=255)
                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:5950866/1‑93 (MQ=255)
                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:5017333/1‑93 (MQ=255)
                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:1312971/1‑93 (MQ=255)
                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:1994334/1‑93 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        >  2:3109564/1‑93 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  1:970984/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  2:1293924/93‑1 (MQ=255)
                                                                  gCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                       <  2:3999511/93‑1 (MQ=255)
                                                                   ccTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcgc                      <  1:5946542/93‑1 (MQ=255)
                                                                    cTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                     <  1:2117306/93‑1 (MQ=255)
                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    <  2:171011/93‑1 (MQ=255)
                                                                       tgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcg                  >  1:3877716/1‑93 (MQ=255)
                                                                         aaGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                >  2:4869937/1‑93 (MQ=255)
                                                                         aaGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                >  2:5340479/1‑93 (MQ=255)
                                                                          aGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt               >  1:6174896/1‑93 (MQ=255)
                                                                          aGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt               >  1:4886506/1‑93 (MQ=255)
                                                                           gTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg              >  2:5721326/1‑93 (MQ=255)
                                                                           gTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg              <  2:6380924/93‑1 (MQ=255)
                                                                             ggCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa            >  2:1876677/1‑93 (MQ=255)
                                                                              gCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           >  2:6698312/1‑93 (MQ=255)
                                                                              gCTGAAATTTCGCACAAAAAATATCGCCAATTGAGTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           <  1:2828033/93‑1 (MQ=255)
                                                                                 gAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc        <  2:5227439/93‑1 (MQ=255)
                                                                                 gAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc        <  2:4234719/93‑1 (MQ=255)
                                                                                  aaaTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa       >  2:3546282/1‑93 (MQ=255)
                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     <  1:4003892/93‑1 (MQ=255)
                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     >  2:2725081/1‑93 (MQ=255)
                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    >  2:4990859/1‑93 (MQ=255)
                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    >  1:3753438/1‑93 (MQ=255)
                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    <  1:5354550/93‑1 (MQ=255)
                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    >  2:2120205/1‑93 (MQ=255)
                                                                                      ttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTc   <  2:1830420/93‑1 (MQ=255)
                                                                                       tCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa  >  1:2336909/1‑93 (MQ=255)
                                                                                       tCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa  >  1:6685923/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483962‑3484147
                                                                                            |                                                                                             
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCA                                                                                              >  SRR4302536.1911865/1‑94 (MQ=60)
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCA                                                                                              <  SRR4302536.6262224/94‑1 (MQ=60)
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCA                                                                                              >  SRR4302536.1736669/1‑94 (MQ=60)
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGGAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCA                                                                                              >  SRR4302536.2029381/1‑94 (MQ=60)
 TTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAC                                                                                             >  SRR4302536.3447210/1‑94 (MQ=60)
    TATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAA                                                                                          >  SRR4302536.4081776/1‑94 (MQ=60)
      TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAA                                                                                        <  SRR4302536.2842922/94‑1 (MQ=60)
       TGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAA                                                                                       <  SRR4302536.4609505/94‑1 (MQ=60)
       TGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAA                                                                                       <  SRR4302536.2440584/94‑1 (MQ=60)
        GGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAATTGGCTGAAATTTCGCACAAAAAAT                                                                                      <  SRR4302536.2202943/94‑1 (MQ=60)
           GAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTC                                                                                   <  SRR4302536.5745974/94‑1 (MQ=60)
           GAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTC                                                                                   >  SRR4302536.1686341/1‑94 (MQ=60)
            AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  <  SRR4302536.2741302/94‑1 (MQ=60)
            AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  <  SRR4302536.358633/94‑1 (MQ=60)
              CTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCC                                                                                >  SRR4302536.4888059/1‑94 (MQ=60)
               TGGTCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCA                                                                               <  SRR4302536.5327261/94‑1 (MQ=60)
               TGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCA                                                                               <  SRR4302536.5005766/94‑1 (MQ=60)
               TGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCA                                                                               <  SRR4302536.3008922/94‑1 (MQ=60)
               TGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCA                                                                               >  SRR4302536.6483882/1‑94 (MQ=60)
               TGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCCCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCGA                                                                               <  SRR4302536.2282964/94‑1 (MQ=60)
                  GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            <  SRR4302536.4168795/94‑1 (MQ=60)
                  GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            <  SRR4302536.2908402/94‑1 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAACAATTTCGCCAATTG                                                                           <  SRR4302536.6795318/94‑1 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           >  SRR4302536.3373778/1‑94 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           >  SRR4302536.6634235/1‑94 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           <  SRR4302536.3474964/94‑1 (MQ=60)
                     TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAT                                                                         >  SRR4302536.2848727/1‑94 (MQ=60)
                     TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAT                                                                         <  SRR4302536.4495478/94‑1 (MQ=60)
                     TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTCAT                                                                         <  SRR4302536.6814458/94‑1 (MQ=60)
                       TTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTC                                                                       <  SRR4302536.4326550/94‑1 (MQ=60)
                       TGTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAATCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTC                                                                       <  SRR4302536.6120951/94‑1 (MQ=60)
                         TGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAG                                                                     <  SRR4302536.2243787/94‑1 (MQ=60)
                         TAAAGAGGTCCAGGTACGTAGCGTATGGGTACGTGCGAAAACCGCCTGTGAAGTGCCTGAAATTTTGCCCAAAAAATTTCGCCAATTGATTCAG                                                                     >  SRR4302536.1005104/1‑94 (MQ=60)
                          GAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGG                                                                    <  SRR4302536.6203692/94‑1 (MQ=60)
                            AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  <  SRR4302536.4926459/94‑1 (MQ=60)
                            AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  <  SRR4302536.563224/94‑1 (MQ=60)
                              AGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAA                                                                >  SRR4302536.5099993/1‑94 (MQ=60)
                                GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              <  SRR4302536.3324668/94‑1 (MQ=60)
                                 GCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCC                                                             >  SRR4302536.3804176/1‑94 (MQ=60)
                                  CCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCG                                                            >  SRR4302536.4660201/1‑94 (MQ=60)
                                   CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGG                                                           >  SRR4302536.5170872/1‑94 (MQ=60)
                                   CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGG                                                           <  SRR4302536.2903866/94‑1 (MQ=60)
                                    AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302536.4449343/1‑94 (MQ=60)
                                     GGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAC                                                         <  SRR4302536.2172766/94‑1 (MQ=60)
                                      GAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACA                                                        <  SRR4302536.80320/94‑1 (MQ=60)
                                      GAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACA                                                        <  SRR4302536.4084450/94‑1 (MQ=60)
                                      GAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACA                                                        <  SRR4302536.5416287/94‑1 (MQ=60)
                                       AACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAT                                                       <  SRR4302536.4811824/94‑1 (MQ=60)
                                       AACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAT                                                       >  SRR4302536.2828135/1‑94 (MQ=60)
                                       AACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAT                                                       >  SRR4302536.6910279/1‑94 (MQ=60)
                                        ACGTAGCGCAGGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCCCAAAAAATCTCGCCAATTGATTCAGGTAAACCCGGACATT                                                      <  SRR4302536.470416/94‑1 (MQ=60)
                                          GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    <  SRR4302536.2168019/94‑1 (MQ=60)
                                          GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    >  SRR4302536.68292/1‑94 (MQ=60)
                                          GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    <  SRR4302536.2561343/94‑1 (MQ=60)
                                           TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   <  SRR4302536.1355608/94‑1 (MQ=60)
                                           TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   >  SRR4302536.2822135/1‑94 (MQ=60)
                                              CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                >  SRR4302536.4042248/1‑94 (MQ=60)
                                              CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                >  SRR4302536.1135517/1‑94 (MQ=60)
                                              CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                <  SRR4302536.1416087/94‑1 (MQ=60)
                                               GCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGC                                               >  SRR4302536.5110435/1‑94 (MQ=60)
                                                CATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCG                                              <  SRR4302536.3590436/94‑1 (MQ=60)
                                                 ATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGT                                             <  SRR4302536.5008529/94‑1 (MQ=60)
                                                 ATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGT                                             <  SRR4302536.3122349/94‑1 (MQ=60)
                                                 ATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGT                                             >  SRR4302536.3083057/1‑94 (MQ=60)
                                                  TGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            <  SRR4302536.6043750/94‑1 (MQ=60)
                                                  TGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            <  SRR4302536.1057437/94‑1 (MQ=60)
                                                  TGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            >  SRR4302536.1885475/1‑94 (MQ=60)
                                                  AGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            <  SRR4302536.6586811/94‑1 (MQ=60)
                                                   GGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTT                                           <  SRR4302536.118903/94‑1 (MQ=60)
                                                    GGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          >  SRR4302536.4717565/1‑94 (MQ=60)
                                                    GGTACGTCCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          <  SRR4302536.668362/94‑1 (MQ=60)
                                                    GGCACGTGCGACAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          <  SRR4302536.5543213/94‑1 (MQ=60)
                                                       ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302536.6047070/94‑1 (MQ=60)
                                                       ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302536.2533621/94‑1 (MQ=60)
                                                         GTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGC                                     >  SRR4302536.4099710/1‑94 (MQ=60)
                                                         GTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGC                                     <  SRR4302536.6179496/94‑1 (MQ=60)
                                                         GTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGC                                     >  SRR4302536.4603146/1‑94 (MQ=60)
                                                          TGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCA                                    <  SRR4302536.4613209/94‑1 (MQ=60)
                                                          TGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCA                                    <  SRR4302536.6672203/94‑1 (MQ=60)
                                                          TGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCA                                    <  SRR4302536.3912721/94‑1 (MQ=60)
                                                            CGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACA                                  >  SRR4302536.6756007/1‑94 (MQ=60)
                                                             GAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAG                                 <  SRR4302536.1532291/94‑1 (MQ=60)
                                                             GAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAG                                 >  SRR4302536.2661017/1‑94 (MQ=60)
                                                              AAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGA                                >  SRR4302536.3285469/1‑94 (MQ=60)
                                                              AAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGA                                <  SRR4302536.2110889/94‑1 (MQ=60)
                                                               AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               >  SRR4302536.1736113/1‑94 (MQ=60)
                                                               AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302536.5433210/94‑1 (MQ=60)
                                                                 ACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGG                             <  SRR4302536.258219/94‑1 (MQ=60)
                                                                  CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302536.124483/94‑1 (MQ=60)
                                                                  CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            >  SRR4302536.5094952/1‑94 (MQ=60)
                                                                  CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302536.206423/94‑1 (MQ=60)
                                                                   CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           <  SRR4302536.999636/94‑1 (MQ=60)
                                                                    GCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGC                          >  SRR4302536.1215953/1‑94 (MQ=60)
                                                                    GCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGC                          <  SRR4302536.2902877/94‑1 (MQ=60)
                                                                    GCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGC                          <  SRR4302536.1771292/94‑1 (MQ=60)
                                                                     CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         <  SRR4302536.6123714/94‑1 (MQ=60)
                                                                      CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        <  SRR4302536.6855526/94‑1 (MQ=60)
                                                                      CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        >  SRR4302536.4462740/1‑94 (MQ=60)
                                                                      CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        <  SRR4302536.2180925/94‑1 (MQ=60)
                                                                      CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        >  SRR4302536.6155711/1‑94 (MQ=60)
                                                                       TGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTC                       >  SRR4302536.2348288/1‑94 (MQ=60)
                                                                        GTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCG                      >  SRR4302536.3993089/1‑94 (MQ=60)
                                                                         TGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGT                     >  SRR4302536.67641/1‑94 (MQ=60)
                                                                         TGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGT                     >  SRR4302536.81423/1‑94 (MQ=60)
                                                                           AAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCT                   >  SRR4302536.5033492/1‑94 (MQ=60)
                                                                           AAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCT                   >  SRR4302536.6358812/1‑94 (MQ=60)
                                                                           AAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCT                   <  SRR4302536.3766385/94‑1 (MQ=60)
                                                                            AGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTG                  <  SRR4302536.6683910/94‑1 (MQ=60)
                                                                            AGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTG                  >  SRR4302536.5846646/1‑94 (MQ=60)
                                                                            AGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTG                  <  SRR4302536.2179995/94‑1 (MQ=60)
                                                                             GTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGC                 >  SRR4302536.1856340/1‑94 (MQ=60)
                                                                               GGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAA               <  SRR4302536.2479292/94‑1 (MQ=60)
                                                                                GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              <  SRR4302536.5357061/94‑1 (MQ=60)
                                                                                GCTGAAATTTCGCACAAAAAATATCGCCAATTGAGTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              <  SRR4302536.2911757/94‑1 (MQ=60)
                                                                                 CTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGT             >  SRR4302536.5205981/1‑94 (MQ=60)
                                                                                  cgaatTTTCGCACAAAAAACTTCGCCAATTGATTCACGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            <  SRR4302536.982463/89‑1 (MQ=60)
                                                                                  TGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            >  SRR4302536.4567252/1‑94 (MQ=60)
                                                                                  TGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            >  SRR4302536.3877272/1‑94 (MQ=60)
                                                                                     AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         <  SRR4302536.6529157/94‑1 (MQ=60)
                                                                                     AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         <  SRR4302536.4207599/94‑1 (MQ=60)
                                                                                     AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         <  SRR4302536.5375430/94‑1 (MQ=60)
                                                                                      ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        >  SRR4302536.256401/1‑94 (MQ=60)
                                                                                      ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        <  SRR4302536.4122704/94‑1 (MQ=60)
                                                                                      ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        >  SRR4302536.3865581/1‑94 (MQ=60)
                                                                                      ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        >  SRR4302536.1463373/1‑94 (MQ=60)
                                                                                       TTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTC       <  SRR4302536.6330158/94‑1 (MQ=60)
                                                                                       TTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTC       <  SRR4302536.3206316/94‑1 (MQ=60)
                                                                                       TTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTC       <  SRR4302536.5514353/94‑1 (MQ=60)
                                                                                        TTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA      >  SRR4302536.6884489/1‑94 (MQ=60)
                                                                                        TTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA      >  SRR4302536.5905688/1‑94 (MQ=60)
                                                                                        TTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA      >  SRR4302536.2407806/1‑94 (MQ=60)
                                                                                            CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  SRR4302536.3764824/1‑94 (MQ=60)
                                                                                            CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  SRR4302536.403960/1‑94 (MQ=60)
                                                                                            |                                                                                             
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483962‑3484147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑