Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,510,121 +G intergenic (‑358/+151) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,1201.G94.7% 586.4 / 24.0 190intergenic (‑357/+152)yhfZ/trpShypothetical protein/tryptophan tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (9/1);  new base G (102/78);  total (111/79)
Fisher's exact test for biased strand distribution p-value = 4.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 219 total aligned reads displayed.

AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209
                                                                                              |                                                                                         
 aGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑g                                                                                          >  1:2034133/1‑93 (MQ=255)
  gATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑gg                                                                                         >  1:59980/1‑92 (MQ=255)
  gATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑gg                                                                                         >  1:2526396/1‑92 (MQ=255)
    tAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑Ggtt                                                                                       >  2:3175829/1‑93 (MQ=255)
    tAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑Ggtt                                                                                       >  1:2488810/1‑93 (MQ=255)
         cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCaga                                                                                   <  2:2114598/93‑1 (MQ=255)
         cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCaga                                                                                   <  2:3477824/93‑1 (MQ=255)
          gcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAACACCGGGTTTC‑CCCGGTTTCagag                                                                                  >  1:2863973/1‑93 (MQ=255)
          gcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCagag                                                                                  >  1:1488650/1‑93 (MQ=255)
           cAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGt                                                                                 <  2:4523035/93‑1 (MQ=255)
            aaCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtg                                                                                >  2:2151662/1‑93 (MQ=255)
            aaCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtg                                                                                <  2:3025861/93‑1 (MQ=255)
            aaCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGAAAATGAGGATAACAAAACCGGGTTTC‑CCCGGTTTCagagcg                                                                                >  2:3290800/1‑91 (MQ=255)
              cAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgat                                                                              <  1:2323584/93‑1 (MQ=255)
              cAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgat                                                                              >  2:1144955/1‑93 (MQ=255)
              cAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgat                                                                              >  2:3525516/1‑93 (MQ=255)
               aGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgatg                                                                             <  1:1589090/93‑1 (MQ=255)
                gCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgatga                                                                            >  2:1021539/1‑93 (MQ=255)
                     aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATaaaa                                                                       <  1:4112959/93‑1 (MQ=255)
                     aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGGTTC‑CCCGGTTTCAGAGTGATGATaaaa                                                                       >  1:78485/1‑93 (MQ=255)
                       gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGc                                                                     >  2:3652694/1‑93 (MQ=255)
                        tgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCa                                                                    >  2:615024/1‑93 (MQ=255)
                         ggAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCaa                                                                   >  1:1090188/1‑93 (MQ=255)
                            atcGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                <  2:4498590/91‑1 (MQ=255)
                            aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                >  1:3472704/1‑93 (MQ=255)
                            aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                >  2:2873496/1‑93 (MQ=255)
                             aCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAtt                                                               <  2:1078814/93‑1 (MQ=255)
                               ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc                                                             <  1:3262291/93‑1 (MQ=255)
                               ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc                                                             <  2:128703/93‑1 (MQ=255)
                                gCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc                                                            <  1:319296/93‑1 (MQ=255)
                                  gAGGCCCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTg                                                          <  2:3974155/93‑1 (MQ=255)
                                    ggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt                                                        >  2:3812256/1‑93 (MQ=255)
                                     gCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCACCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATg                                                       >  1:3827367/1‑93 (MQ=255)
                                     gCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATg                                                       >  2:132832/1‑93 (MQ=255)
                                     gCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATg                                                       >  2:2649416/1‑93 (MQ=255)
                                      cACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgc                                                      <  1:4021480/93‑1 (MQ=255)
                                      cACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgc                                                      <  1:28350/93‑1 (MQ=255)
                                       aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg                                                     >  1:927509/1‑93 (MQ=255)
                                        cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    >  2:1058774/1‑93 (MQ=255)
                                        cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    >  2:1678468/1‑93 (MQ=255)
                                        cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    <  1:4338348/93‑1 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   >  1:682056/1‑93 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   >  2:1967743/1‑93 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGGTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   >  2:3854202/1‑93 (MQ=255)
                                          tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                  >  1:4588504/1‑93 (MQ=255)
                                          tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                  <  2:2314140/93‑1 (MQ=255)
                                           cacaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                 >  1:2424935/1‑93 (MQ=255)
                                              aTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt                                              <  1:3400752/93‑1 (MQ=255)
                                              aTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt                                              >  1:430778/1‑93 (MQ=255)
                                               ttttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAGTGCCTGATGCGCTACGCtt                                             >  1:4204495/1‑93 (MQ=255)
                                                tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa                                            >  2:1429705/1‑93 (MQ=255)
                                                tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa                                            <  1:189944/93‑1 (MQ=255)
                                                 ttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt                                           >  2:1373529/1‑93 (MQ=255)
                                                  tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc                                          >  1:2811109/1‑93 (MQ=255)
                                                  tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc                                          <  1:850543/93‑1 (MQ=255)
                                                  tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc                                          <  2:620125/93‑1 (MQ=255)
                                                   ttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCa                                         <  2:1719763/93‑1 (MQ=255)
                                                    tCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAg                                        >  1:4076155/1‑93 (MQ=255)
                                                    tCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAg                                        <  2:2414605/93‑1 (MQ=255)
                                                     cGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAgg                                       >  2:704512/1‑93 (MQ=255)
                                                       gATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                     <  1:3504154/93‑1 (MQ=255)
                                                       gATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                     >  1:3680073/1‑93 (MQ=255)
                                                       gATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                     >  2:4314302/1‑93 (MQ=255)
                                                       gATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                     >  1:3007110/1‑93 (MQ=255)
                                                           attGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                 <  2:1233459/93‑1 (MQ=255)
                                                           attGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                 <  2:3394593/93‑1 (MQ=255)
                                                           attGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                 >  1:4443690/1‑93 (MQ=255)
                                                           attGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                 <  1:2979092/93‑1 (MQ=255)
                                                            ttGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAt                                <  1:104233/93‑1 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               >  2:440293/1‑93 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               >  1:2186521/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  2:3384273/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  1:446327/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           <  1:587012/93‑1 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  2:4594790/1‑93 (MQ=255)
                                                                  aTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCtt                          >  1:1238829/1‑93 (MQ=255)
                                                                    gAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGc                        >  1:1020900/1‑93 (MQ=255)
                                                                      ggATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCaa                      >  1:4717783/1‑93 (MQ=255)
                                                                       gATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                     >  2:603750/1‑93 (MQ=255)
                                                                          aaaaaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  <  1:730036/93‑1 (MQ=255)
                                                                          aaaaaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  <  1:2641986/93‑1 (MQ=255)
                                                                            aaaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  1:3291690/1‑93 (MQ=255)
                                                                            aaaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  1:1175521/1‑93 (MQ=255)
                                                                             aaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAACAGCAAAATTGCCTGAAGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGc               <  2:1006110/93‑1 (MQ=37)
                                                                             aaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGc               <  2:2256335/93‑1 (MQ=255)
                                                                              aaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              <  1:1697927/93‑1 (MQ=255)
                                                                              aaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCATACATTTCCTTGCAATATGTGCa              <  1:3948721/93‑1 (MQ=255)
                                                                                  cGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAc          <  2:1570068/93‑1 (MQ=255)
                                                                                   gggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACt         >  1:1149213/1‑93 (MQ=255)
                                                                                    ggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACtt        >  2:3560125/1‑93 (MQ=255)
                                                                                    ggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACtt        <  2:3772341/93‑1 (MQ=255)
                                                                                    ggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACtt        >  1:3359101/1‑93 (MQ=255)
                                                                                    ggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACtt        <  2:3115448/93‑1 (MQ=255)
                                                                                     gTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACttt       <  1:3110279/93‑1 (MQ=255)
                                                                                     gTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACttt       >  1:1831842/1‑93 (MQ=255)
                                                                                      ttcc‑ccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGt     <  2:1856877/90‑1 (MQ=255)
                                                                                        tC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    >  2:1045275/1‑93 (MQ=255)
                                                                                        cc‑ccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAg   >  2:67397/2‑93 (MQ=255)
                                                                                        cc‑ccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGGAg   <  2:2399787/92‑1 (MQ=255)
                                                                                         c‑ccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAgg  >  2:4684636/1‑93 (MQ=255)
                                                                                              |                                                                                         
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGG  >  NC_000913/3510028‑3510213
                                                                                               |                                                                                            
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑G                                                                                              <  SRR4302544.34006/94‑1 (MQ=60)
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑G                                                                                              >  SRR4302544.2053804/1‑94 (MQ=60)
 AGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GG                                                                                             >  SRR4302544.2550676/1‑94 (MQ=60)
 AGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GG                                                                                             >  SRR4302544.60591/1‑94 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GGTT                                                                                           >  SRR4302544.2512650/1‑94 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GGTT                                                                                           >  SRR4302544.76461/1‑94 (MQ=60)
    TAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTT                                                                                           >  SRR4302544.2558853/1‑94 (MQ=60)
      AGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCA                                                                                         <  SRR4302544.593357/94‑1 (MQ=60)
        GCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGA                                                                                       >  SRR4302544.4322018/1‑94 (MQ=60)
         CGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAACACCGGGTTTC‑CCCGGTTTCAGAG                                                                                      >  SRR4302544.2891618/1‑94 (MQ=60)
         CGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAG                                                                                      >  SRR4302544.1503367/1‑94 (MQ=60)
              CAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATG                                                                                 <  SRR4302544.2345611/94‑1 (MQ=60)
               AGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGA                                                                                <  SRR4302544.1283391/94‑1 (MQ=60)
               AGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGA                                                                                <  SRR4302544.1604764/94‑1 (MQ=60)
                 CGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATA                                                                              >  SRR4302544.4369155/1‑94 (MQ=60)
                    GATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGGTTC‑CCCGGTTTCAGAGTGATGATAAAA                                                                           >  SRR4302544.79240/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAG                                                                          <  SRR4302544.2256520/94‑1 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAG                                                                          <  SRR4302544.4153828/94‑1 (MQ=60)
                       GTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCA                                                                        >  SRR4302544.1125400/1‑94 (MQ=60)
                        TGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAA                                                                       >  SRR4302544.1101031/1‑94 (MQ=60)
                           AAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAT                                                                    >  SRR4302544.3505822/1‑94 (MQ=60)
                           AAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAT                                                                    >  SRR4302544.1090235/1‑94 (MQ=60)
                               GGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCC                                                                <  SRR4302544.3293446/94‑1 (MQ=60)
                                GCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCT                                                               <  SRR4302544.322616/94‑1 (MQ=60)
                                GCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCT                                                               <  SRR4302544.4496535/94‑1 (MQ=60)
                                GCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCT                                                               <  SRR4302544.4748900/94‑1 (MQ=60)
                                 CGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTG                                                              >  SRR4302544.3460267/1‑94 (MQ=60)
                                  GAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGA                                                             >  SRR4302544.648291/1‑94 (MQ=60)
                                    GGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCACCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATG                                                           >  SRR4302544.3865324/1‑94 (MQ=60)
                                    GGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATG                                                           <  SRR4302544.1041231/94‑1 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                         >  SRR4302544.936648/1‑94 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                         <  SRR4302544.4061514/94‑1 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                         >  SRR4302544.4196175/1‑94 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                         <  SRR4302544.28622/94‑1 (MQ=60)
                                      CACTTCACATTTTTTCGGACTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                         <  SRR4302544.4663367/94‑1 (MQ=60)
                                       ACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                        >  SRR4302544.4111430/1‑94 (MQ=60)
                                       ACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                        >  SRR4302544.3101351/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                       >  SRR4302544.3026093/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                       >  SRR4302544.4138807/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                       <  SRR4302544.4382499/94‑1 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                       >  SRR4302544.688838/1‑94 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                      >  SRR4302544.4634174/1‑94 (MQ=60)
                                          TCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAC                                                     >  SRR4302544.2448016/1‑94 (MQ=60)
                                             CATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCT                                                  >  SRR4302544.4561949/1‑94 (MQ=60)
                                             CATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCT                                                  >  SRR4302544.435141/1‑94 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                 >  SRR4302544.4426770/1‑94 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                 >  SRR4302544.2026922/1‑94 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                 <  SRR4302544.3433270/94‑1 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                 >  SRR4302544.2436552/1‑94 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAGTGCCTGATGCGCTACGCTT                                                 >  SRR4302544.4246618/1‑94 (MQ=60)
                                                TTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAT                                               <  SRR4302544.191840/94‑1 (MQ=60)
                                                 TTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATC                                              <  SRR4302544.3149068/94‑1 (MQ=60)
                                                 TTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATC                                              >  SRR4302544.2838234/1‑94 (MQ=60)
                                                  TTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCA                                             <  SRR4302544.858949/94‑1 (MQ=60)
                                                  TTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCA                                             <  SRR4302544.858947/94‑1 (MQ=60)
                                                  TCTCGGATTATTGCAAATGAGGATAAAAAAACCGGTTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCA                                             <  SRR4302544.2473243/94‑1 (MQ=60)
                                                   TTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAG                                            >  SRR4302544.4116718/1‑94 (MQ=60)
                                                   TTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAG                                            >  SRR4302544.2539683/1‑94 (MQ=60)
                                                     CGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGC                                          >  SRR4302544.3739123/1‑94 (MQ=60)
                                                      GGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCC                                         >  SRR4302544.3035934/1‑94 (MQ=60)
                                                      GGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCC                                         >  SRR4302544.3716778/1‑94 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                        >  SRR4302544.3510285/1‑94 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                        >  SRR4302544.1849436/1‑94 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                        <  SRR4302544.3537579/94‑1 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                        <  SRR4302544.3375853/94‑1 (MQ=60)
                                                       GATTAGTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                        <  SRR4302544.3375852/94‑1 (MQ=60)
                                                         TTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAC                                      <  SRR4302544.3765682/94‑1 (MQ=60)
                                                          TATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACA                                     >  SRR4302544.4488482/1‑94 (MQ=60)
                                                           ATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAT                                    <  SRR4302544.3007664/94‑1 (MQ=60)
                                                           ATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAT                                    >  SRR4302544.1708209/1‑94 (MQ=60)
                                                            TTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATT                                   >  SRR4302544.2207195/1‑94 (MQ=60)
                                                            TTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATT                                   <  SRR4302544.105190/94‑1 (MQ=60)
                                                             TGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTT                                  <  SRR4302544.3582811/94‑1 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                               <  SRR4302544.116292/94‑1 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                               >  SRR4302544.450776/1‑94 (MQ=60)
                                                                 AATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTT                              >  SRR4302544.1251067/1‑94 (MQ=60)
                                                                 AATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTT                              <  SRR4302544.592769/94‑1 (MQ=60)
                                                                 AATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTT                              >  SRR4302544.2945398/1‑94 (MQ=60)
                                                                  ATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTG                             >  SRR4302544.3298146/1‑94 (MQ=60)
                                                                  ATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTG                             >  SRR4302544.4211284/1‑94 (MQ=60)
                                                                   TGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGC                            >  SRR4302544.1031089/1‑94 (MQ=60)
                                                                     AGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAA                          >  SRR4302544.4764383/1‑94 (MQ=60)
                                                                     AGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAA                          >  SRR4302544.767032/1‑94 (MQ=60)
                                                                      GGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT                         >  SRR4302544.1565729/1‑94 (MQ=60)
                                                                      GGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT                         <  SRR4302544.1446386/94‑1 (MQ=60)
                                                                       GATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATA                        <  SRR4302544.1192718/94‑1 (MQ=60)
                                                                       GATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATA                        <  SRR4302544.708393/94‑1 (MQ=60)
                                                                          AAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGT                     <  SRR4302544.737185/94‑1 (MQ=60)
                                                                          AAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGT                     <  SRR4302544.2667313/94‑1 (MQ=60)
                                                                           AAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTG                    >  SRR4302544.1187240/1‑94 (MQ=60)
                                                                           AAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTG                    >  SRR4302544.3323051/1‑94 (MQ=60)
                                                                             AAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                  <  SRR4302544.2868968/94‑1 (MQ=60)
                                                                             AAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                  >  SRR4302544.2801166/1‑94 (MQ=60)
                                                                              AAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAT                 <  SRR4302544.1714681/94‑1 (MQ=60)
                                                                              AAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCATACATTTCCTTGCAATATGTGCAT                 <  SRR4302544.3987963/94‑1 (MQ=60)
                                                                                 CCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAC              <  SRR4302544.889116/94‑1 (MQ=60)
                                                                                  CGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACT             >  SRR4302544.1160644/1‑94 (MQ=60)
                                                                                  CGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACT             <  SRR4302544.2860219/94‑1 (MQ=60)
                                                                                   GGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTT            >  SRR4302544.3391157/1‑94 (MQ=60)
                                                                                   GGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAGCAGGCTTACATTTCCTTGCAATATGTGCGTTACTT            >  SRR4302544.521703/1‑94 (MQ=60)
                                                                                    GGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTT           >  SRR4302544.1849649/1‑94 (MQ=60)
                                                                                     GTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTG          <  SRR4302544.3139874/94‑1 (MQ=60)
                                                                                     GTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTG          <  SRR4302544.4050612/94‑1 (MQ=60)
                                                                                      TTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGT         >  SRR4302544.3968577/1‑94 (MQ=60)
                                                                                        TC‑CCG‑GTTTCAGAGTGCTGATAAGAGCAAGATTTCCTGATGCGCGACGCTTATCGGGCGTACATTTCCTTGCAATATGTGCATTTCTTTGTAGG      <  SRR4302544.193155/94‑1 (MQ=53)
                                                                                        TC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAG       <  SRR4302544.3022894/94‑1 (MQ=60)
                                                                                         C‑CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGC     >  SRR4302544.1124890/1‑94 (MQ=60)
                                                                                         C‑CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGC     >  SRR4302544.1430977/1‑94 (MQ=60)
                                                                                         C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGTTGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG      <  SRR4302544.193025/94‑1 (MQ=60)
                                                                                         C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG      >  SRR4302544.3245486/1‑94 (MQ=60)
                                                                                         C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG      <  SRR4302544.1916647/94‑1 (MQ=60)
                                                                                         C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG      <  SRR4302544.2170386/94‑1 (MQ=60)
                                                                                         C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG      >  SRR4302544.2537571/1‑94 (MQ=60)
                                                                                           CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTTCATTACGTTGTAGGTC    >  SRR4302544.4370246/1‑94 (MQ=60)
                                                                                            CG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG   >  SRR4302544.1137890/1‑94 (MQ=60)
                                                                                            CG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG   >  SRR4302544.2317301/1‑94 (MQ=60)
                                                                                            CG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG   >  SRR4302544.2317302/1‑94 (MQ=60)
                                                                                             G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGG  >  SRR4302544.452278/1‑94 (MQ=60)
                                                                                               |                                                                                            
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGG  >  NC_000913/3510028‑3510213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑