Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,539,774 G→A A578T (GCC→ACC)  feoB → ferrous iron transport protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,539,7740GA97.1% 470.4 / ‑0.4 139A578T (GCC→ACC) feoBferrous iron transport protein B
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (60/74);  total (62/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 140 total aligned reads displayed.

GCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTT  >  NC_000913/3539684‑3539863
                                                                                          |                                                                                         
gCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTAcc                                                                                         <  1:4723422/93‑1 (MQ=255)
  gtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCAt                                                                                       <  1:2477373/93‑1 (MQ=255)
  gtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCAt                                                                                       >  2:437681/1‑93 (MQ=255)
   tccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      >  1:119760/1‑93 (MQ=255)
   tccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      <  1:1276386/93‑1 (MQ=255)
   gccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      <  2:4577572/92‑1 (MQ=255)
   gccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      <  1:94784/92‑1 (MQ=255)
    ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATgg                                                                                     <  1:3142034/93‑1 (MQ=255)
    ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATgg                                                                                     >  1:4047743/1‑93 (MQ=255)
     cgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGTTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGc                                                                                    <  1:1406170/93‑1 (MQ=255)
     cgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGc                                                                                    >  2:2160033/1‑93 (MQ=255)
       tcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGa                                                                                  <  1:1271346/93‑1 (MQ=255)
         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCggag                                                                                >  2:1477434/1‑90 (MQ=255)
         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGaaa                                                                                >  2:4498432/1‑93 (MQ=255)
          gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaag                                                                               <  1:3981852/93‑1 (MQ=255)
            cGGGTGATCCCCCCGGTCTGCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaagaa                                                                             <  2:1981116/93‑1 (MQ=255)
             gggTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaagaag                                                                            >  1:949351/1‑93 (MQ=255)
             gggTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaagaag                                                                            >  1:4669130/1‑93 (MQ=255)
                tGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTgg                                                                         <  2:1387674/93‑1 (MQ=255)
                 gaccACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGt                                                                        <  1:1178295/90‑1 (MQ=255)
                 gATCCCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGt                                                                        <  2:1888802/93‑1 (MQ=255)
                 gATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGt                                                                        <  2:2647882/93‑1 (MQ=255)
                 gATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGt                                                                        <  2:1888801/93‑1 (MQ=255)
                   tcgccccGGTCTACAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAg                                                                      <  1:3506682/90‑1 (MQ=255)
                    cACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGt                                                                     <  2:4719654/93‑1 (MQ=255)
                    cACCCCGGTCTTCAAGCCAATTGCCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGt                                                                     <  2:2589853/93‑1 (MQ=255)
                     aCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTg                                                                    >  2:356936/1‑93 (MQ=255)
                       cccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTggg                                                                  <  2:2059808/93‑1 (MQ=255)
                         cGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                                                                >  1:4527716/1‑93 (MQ=255)
                          ggTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTAc                                                               <  1:1979542/93‑1 (MQ=255)
                           gTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACg                                                              <  1:2976174/93‑1 (MQ=255)
                              ttCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTc                                                           >  1:4117923/1‑93 (MQ=255)
                                cAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCaa                                                         <  1:3497367/93‑1 (MQ=255)
                                 aaGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                                                        <  1:2682420/93‑1 (MQ=255)
                                   gCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAacac                                                      >  2:1365776/1‑93 (MQ=255)
                                   gCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGCGGTAGTGGGTACGCTCAacac                                                      >  2:371360/1‑93 (MQ=255)
                                    ccAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAcc                                                     <  1:4040140/93‑1 (MQ=255)
                                     cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc                                                    <  2:1255484/93‑1 (MQ=255)
                                     cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc                                                    <  1:42435/93‑1 (MQ=255)
                                      aaTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                                                   >  1:2336000/1‑93 (MQ=255)
                                       aTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc                                                  >  1:1521063/1‑93 (MQ=255)
                                       aTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc                                                  <  2:4668734/93‑1 (MQ=255)
                                        ttGGCGTGCATGAAGATAACTGGCATGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct                                                 >  1:4533159/1‑93 (MQ=255)
                                        ttGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct                                                 <  2:1089461/93‑1 (MQ=255)
                                        ttGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct                                                 >  1:1941784/1‑93 (MQ=255)
                                        ttGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct                                                 >  1:276337/1‑93 (MQ=255)
                                           gCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTaca                                              <  2:4355929/93‑1 (MQ=255)
                                            cGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac                                             <  2:3123997/93‑1 (MQ=255)
                                              tGCATGAAGATAACTGGCAGGCAGCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg                                           <  2:250385/93‑1 (MQ=255)
                                               gCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTCAACCga                                          >  1:4038661/1‑92 (MQ=255)
                                                 aTGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAg                                        >  1:685404/1‑93 (MQ=255)
                                                 aTGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAg                                        <  2:4582538/93‑1 (MQ=255)
                                                  tGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa                                       >  1:4748276/1‑93 (MQ=255)
                                                  tGAAGATAACTGGCAGGCAACGGTCGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa                                       >  1:1839048/1‑93 (MQ=255)
                                                   gAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaa                                      >  1:4749496/1‑93 (MQ=255)
                                                   gAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaa                                      >  2:1218758/1‑93 (MQ=255)
                                                       aTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAata                                  <  1:2785708/93‑1 (MQ=255)
                                                        tAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAatat                                 >  1:2667248/1‑93 (MQ=255)
                                                          aCTTGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  1:2325742/93‑1 (MQ=255)
                                                          aCTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATAtta                               >  1:3639421/1‑92 (MQ=255)
                                                          aCTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  2:1054784/93‑1 (MQ=255)
                                                           cTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCa                              <  1:2294578/93‑1 (MQ=255)
                                                           cTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCa                              >  1:152232/1‑93 (MQ=255)
                                                           cTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCa                              <  2:4679736/93‑1 (MQ=255)
                                                           cTGGCAGGCAACGGTTGGCCTGTTTACAGGGCCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCa                              <  1:2294579/93‑1 (MQ=255)
                                                            tGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAg                             >  2:148858/1‑93 (MQ=255)
                                                             ggcaggcaACGGTTGGCCTGTTTACAGGTACCATGGGGAACGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCTCAGAAAATATTCAgg                            >  2:1063032/1‑93 (MQ=255)
                                                             ggcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAgg                            >  1:4381433/1‑93 (MQ=255)
                                                               caggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAggct                          >  2:3408346/1‑91 (MQ=255)
                                                               caggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGAc                          <  2:743673/93‑1 (MQ=255)
                                                               caggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGAc                          <  2:1788799/93‑1 (MQ=255)
                                                                 ggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGa                        >  1:3628175/1‑93 (MQ=255)
                                                                 ggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGa                        <  1:3829920/93‑1 (MQ=255)
                                                                 ggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGa                        >  2:4267557/1‑93 (MQ=255)
                                                                   caACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAag                      <  2:3125494/93‑1 (MQ=255)
                                                                    aaCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAaga                     <  2:1959099/93‑1 (MQ=255)
                                                                    aaCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAaga                     <  2:1103230/93‑1 (MQ=255)
                                                                    aaCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAaga                     >  1:741233/1‑93 (MQ=255)
                                                                    aaCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAaga                     <  2:2830263/93‑1 (MQ=255)
                                                                     aCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAagag                    >  1:4623651/1‑93 (MQ=255)
                                                                      cGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGt                   >  1:4655760/1‑93 (MQ=255)
                                                                        gTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTc                 >  1:1146007/1‑93 (MQ=255)
                                                                        gTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTc                 <  2:1784672/93‑1 (MQ=255)
                                                                        gTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTc                 <  1:3674809/93‑1 (MQ=255)
                                                                          tGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCaa               >  2:3114555/1‑93 (MQ=255)
                                                                          tGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCaa               <  1:1799333/93‑1 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTGAACAGCCTCTACACCGCAGAAAATATTCAGGACGAAGACGTCAATc             >  2:2434130/1‑93 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATc             <  2:588498/93‑1 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATc             <  2:4242870/93‑1 (MQ=255)
                                                                             ccTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATcc            >  1:899089/1‑93 (MQ=255)
                                                                             ccTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATcc            <  1:1890420/93‑1 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          <  2:397348/93‑1 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          >  1:3551101/1‑93 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          >  2:1194894/1‑93 (MQ=255)
                                                                                 tttACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCa        <  1:4388879/93‑1 (MQ=255)
                                                                                 tttACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCa        <  1:3307999/93‑1 (MQ=255)
                                                                                 tttACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCa        <  2:3761565/93‑1 (MQ=255)
                                                                                    aCAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGaa     <  2:1749071/93‑1 (MQ=255)
                                                                                       ggTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCTGGACGAAGAGTTCAATCCGGCAGAAttt  >  2:1086292/1‑93 (MQ=255)
                                                                                       ggTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAAttt  <  2:2701478/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTT  >  NC_000913/3539684‑3539863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAACC  >  NC_000913/3539682‑3539867
                                                                                            |                                                                                             
TGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTAC                                                                                              >  SRR4302544.2637027/1‑94 (MQ=60)
  GCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCA                                                                                            <  SRR4302544.4770057/94‑1 (MQ=60)
    GTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATG                                                                                          <  SRR4302544.3688200/94‑1 (MQ=60)
    GTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATG                                                                                          >  SRR4302544.120847/1‑94 (MQ=60)
    GTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATG                                                                                          <  SRR4302544.2501064/94‑1 (MQ=60)
     TCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTTGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGG                                                                                         >  SRR4302544.4088057/1‑94 (MQ=60)
     TCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGG                                                                                         <  SRR4302544.1288954/94‑1 (MQ=60)
     TCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGG                                                                                         >  SRR4302544.4088060/1‑94 (MQ=60)
     GCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGG                                                                                         <  SRR4302544.95658/94‑1 (MQ=60)
      CCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGC                                                                                        <  SRR4302544.3171904/94‑1 (MQ=60)
       CGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGTTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCG                                                                                       <  SRR4302544.1420174/94‑1 (MQ=60)
        GTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGA                                                                                      <  SRR4302544.842544/94‑1 (MQ=60)
         TCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAA                                                                                     <  SRR4302544.1283852/94‑1 (MQ=60)
            GCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGA                                                                                  <  SRR4302544.4021482/94‑1 (MQ=60)
              CGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAG                                                                                >  SRR4302544.4715290/1‑94 (MQ=60)
              CGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAG                                                                                >  SRR4302544.958690/1‑94 (MQ=60)
                 GTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGG                                                                             <  SRR4302544.1358976/94‑1 (MQ=60)
                  TGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGT                                                                            <  SRR4302544.4056739/94‑1 (MQ=60)
                   GACCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTA                                                                           <  SRR4302544.1190037/94‑1 (MQ=60)
                     TCGCCCCGGTCTACAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGT                                                                         <  SRR4302544.3540133/94‑1 (MQ=60)
                      CACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTG                                                                        >  SRR4302544.4737276/1‑94 (MQ=60)
                          CCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTA                                                                    >  SRR4302544.4573025/1‑94 (MQ=60)
                            GGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACG                                                                  <  SRR4302544.1998772/94‑1 (MQ=60)
                            GGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACG                                                                  >  SRR4302544.577125/1‑94 (MQ=60)
                            GGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACG                                                                  >  SRR4302544.2747062/1‑94 (MQ=60)
                             GTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGC                                                                 <  SRR4302544.3004722/94‑1 (MQ=60)
                               CTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTC                                                               >  SRR4302544.4158813/1‑94 (MQ=60)
                                  CAAGCCAATTGGTGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAAC                                                            <  SRR4302544.1260759/94‑1 (MQ=60)
                                  CAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAAC                                                            <  SRR4302544.3530719/94‑1 (MQ=60)
                                  CAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAAGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAAC                                                            >  SRR4302544.974378/1‑94 (MQ=60)
                                   AAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACA                                                           <  SRR4302544.2708125/94‑1 (MQ=60)
                                      CCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCC                                                        <  SRR4302544.4080370/94‑1 (MQ=60)
                                       CAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCT                                                       >  SRR4302544.2358141/1‑94 (MQ=60)
                                       CAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCT                                                       <  SRR4302544.42853/94‑1 (MQ=60)
                                        AATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTC                                                      >  SRR4302544.2501752/1‑94 (MQ=60)
                                        AATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTC                                                      >  SRR4302544.1536158/1‑94 (MQ=60)
                                         ATTGGCGTGCATGAAGATAACTGGCATGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCT                                                     >  SRR4302544.4578494/1‑94 (MQ=60)
                                         ATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCT                                                     >  SRR4302544.1960576/1‑94 (MQ=60)
                                         ATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCT                                                     >  SRR4302544.2316423/1‑94 (MQ=60)
                                         ATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCT                                                     >  SRR4302544.279231/1‑94 (MQ=60)
                                                TGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTcaaccga                                              >  SRR4302544.4078879/1‑87 (MQ=60)
                                                  CATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAG                                            <  SRR4302544.3424722/94‑1 (MQ=60)
                                                  CATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAG                                            >  SRR4302544.692222/1‑94 (MQ=60)
                                                   ATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGA                                           >  SRR4302544.4795046/1‑94 (MQ=60)
                                                   ATGAAGATAACTGGCAGGCAACGGTCGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGA                                           >  SRR4302544.1856890/1‑94 (MQ=60)
                                                    TGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAA                                          >  SRR4302544.4796275/1‑94 (MQ=60)
                                                         ATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATAT                                     <  SRR4302544.2812516/94‑1 (MQ=60)
                                                         ATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATAT                                     >  SRR4302544.2692818/1‑94 (MQ=60)
                                                          TAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATT                                    >  SRR4302544.4402694/1‑94 (MQ=60)
                                                           AACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTA                                   >  SRR4302544.3675730/1‑94 (MQ=60)
                                                            ACTTGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCA                                  <  SRR4302544.2347789/94‑1 (MQ=60)
                                                            ACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCA                                  >  SRR4302544.153709/1‑94 (MQ=60)
                                                             CTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAG                                 <  SRR4302544.2316208/94‑1 (MQ=60)
                                                             CTGGCAGGCAACGGTTGGCCTGTTTACAGGGCCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAG                                 <  SRR4302544.2316209/94‑1 (MQ=60)
                                                              TGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGG                                >  SRR4302544.4425816/1‑94 (MQ=60)
                                                                  AGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGA                            >  SRR4302544.2211712/1‑94 (MQ=60)
                                                                  AGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGA                            >  SRR4302544.3664321/1‑94 (MQ=60)
                                                                   GGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAA                           <  SRR4302544.3867897/94‑1 (MQ=60)
                                                                     CAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGA                         >  SRR4302544.748490/1‑94 (MQ=60)
                                                                      AACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAG                        >  SRR4302544.4669519/1‑94 (MQ=60)
                                                                      AACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAG                        >  SRR4302544.4669594/1‑94 (MQ=60)
                                                                       ACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGT                       >  SRR4302544.4701828/1‑94 (MQ=60)
                                                                         GGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTC                     >  SRR4302544.1157408/1‑94 (MQ=60)
                                                                         GGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTC                     >  SRR4302544.3055994/1‑94 (MQ=60)
                                                                         GGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTC                     >  SRR4302544.4521188/1‑94 (MQ=60)
                                                                         GGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTC                     >  SRR4302544.1906138/1‑94 (MQ=60)
                                                                          GTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCA                    <  SRR4302544.3711456/94‑1 (MQ=60)
                                                                            TGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAAT                  <  SRR4302544.1816920/94‑1 (MQ=60)
                                                                              GCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCC                <  SRR4302544.1604726/94‑1 (MQ=60)
                                                                              GCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCC                >  SRR4302544.907985/1‑94 (MQ=60)
                                                                              GCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGGGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTGCAATCC                >  SRR4302544.3631102/1‑94 (MQ=60)
                                                                               CCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCG               <  SRR4302544.1908683/94‑1 (MQ=60)
                                                                                CTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGG              >  SRR4302544.3585111/1‑94 (MQ=60)
                                                                                   TTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAG           <  SRR4302544.4433304/94‑1 (MQ=60)
                                                                                   TTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAG           <  SRR4302544.3339502/94‑1 (MQ=60)
                                                                                          GTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAA    <  SRR4302544.179363/94‑1 (MQ=60)
                                                                                           TACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAAC   <  SRR4302544.4526688/94‑1 (MQ=60)
                                                                                           TACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAAC   <  SRR4302544.569931/94‑1 (MQ=60)
                                                                                           TACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAAC   <  SRR4302544.2227287/94‑1 (MQ=60)
                                                                                           TACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAAC   >  SRR4302544.1053258/1‑94 (MQ=60)
                                                                                           TACCATGGAGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAAC   <  SRR4302544.4526689/94‑1 (MQ=60)
                                                                                            ACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAACC  <  SRR4302544.2643378/94‑1 (MQ=60)
                                                                                            |                                                                                             
TGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTTAACC  >  NC_000913/3539682‑3539867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑