Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,227,795	A→G	intergenic (‑74/‑138)	pepE ← / → yjbC	peptidase E/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,227,795	0	A	G	98.1%	656.2 / 0.9	214	intergenic (‑74/‑138)	pepE/yjbC	peptidase E/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (105/105); total (107/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 231 total aligned reads displayed.

AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTT  >  NC_000913/4227705‑4227884

 aTAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGa                                                                                        <  1:1115375/93‑1 (MQ=255)

 aTAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGa                                                                                        >  2:1640513/1‑93 (MQ=255)

  tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTGCCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAt                                                                                       >  2:3895720/1‑93 (MQ=255)

   aaaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATg                                                                                      <  1:3770730/93‑1 (MQ=255)

    aaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGt                                                                                     >  1:622897/1‑93 (MQ=255)

    aaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGt                                                                                     >  1:2924393/1‑93 (MQ=255)

     aaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGGTCGCGATGTc                                                                                    <  1:2762664/93‑1 (MQ=255)

       gCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGc                                                                                  >  2:4479639/1‑93 (MQ=255)

        cAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGca                                                                                 <  1:74198/93‑1 (MQ=255)

          gTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGCTGTCGcacc                                                                               >  2:4426482/1‑92 (MQ=255)

          gTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGcaca                                                                               >  2:930607/1‑93 (MQ=255)

          gTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGcaca                                                                               >  2:4310949/1‑93 (MQ=255)

           ttCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAt                                                                              <  1:1653706/93‑1 (MQ=255)

              cATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGc                                                                           <  2:1077532/93‑1 (MQ=255)

               aTTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCt                                                                          <  2:426147/93‑1 (MQ=255)

                ttACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCtt                                                                         <  2:806112/93‑1 (MQ=255)

                ttACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCtt                                                                         >  2:1681544/1‑93 (MQ=255)

                ttACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCAAATTGCtt                                                                         >  1:4506591/1‑93 (MQ=255)

                 tACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTc                                                                        <  2:1025315/93‑1 (MQ=255)

                   cTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTg                                                                      >  2:1320688/1‑93 (MQ=255)

                     tctcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGa                                                                    >  1:1746302/1‑93 (MQ=255)

                      ctcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATCTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGaa                                                                   <  1:2699419/93‑1 (MQ=255)

                       tcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGaaa                                                                  <  2:614032/93‑1 (MQ=255)

                        ccGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAg                                                                 <  2:2031924/93‑1 (MQ=255)

                         cGGTTAGTAAGAAAGCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACGGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGt                                                                <  2:29858/93‑1 (MQ=255)

                          ggTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTg                                                               >  2:2006665/1‑93 (MQ=255)

                           gTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGa                                                              >  2:3644429/1‑93 (MQ=255)

                           gTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGa                                                              <  1:412169/93‑1 (MQ=255)

                              aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAttt                                                           >  1:2716386/1‑93 (MQ=255)

                              aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAttt                                                           <  2:4102597/93‑1 (MQ=255)

                               gTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTg                                                          >  1:4586984/1‑93 (MQ=255)

                                tAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCATATTTCTTCTGGAAAGTGATTTGa                                                         >  2:1605354/1‑93 (MQ=255)

                                tAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGa                                                         <  1:2686816/93‑1 (MQ=255)

                                tAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAAGGATTTGa                                                         >  1:1147143/1‑93 (MQ=255)

                                tAAAAAATCACGGGAGGCATCCTACCACTGCGAATTTAAACACTTACTGACATGGCTCGAGATGTCGCACATTGCTTCTGGAAAGTGATTTGa                                                         <  1:585294/93‑1 (MQ=255)

                                  aGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGc                                                       >  1:2382205/1‑93 (MQ=255)

                                     aaTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAgg                                                    >  2:3098246/1‑93 (MQ=255)

                                      aTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGt                                                   <  1:3218260/93‑1 (MQ=255)

                                      aTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGt                                                   <  1:859319/93‑1 (MQ=255)

                                        cACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGGCATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTaa                                                 <  1:3971614/93‑1 (MQ=255)

                                         aCGTGAGGCATGCTACCACTGCGAATTGAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAAc                                                <  1:177403/93‑1 (MQ=255)

                                         aCGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAAc                                                <  1:2881892/93‑1 (MQ=255)

                                          cGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAAca                                               >  1:2984122/1‑92 (MQ=255)

                                            ggAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGaa                                             >  1:3101704/1‑93 (MQ=255)

                                              aGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAAtt                                           <  1:4446463/93‑1 (MQ=255)

                                              aGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAAtt                                           <  2:4699461/93‑1 (MQ=255)

                                               ggCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTc                                          >  1:1025309/1‑93 (MQ=255)

                                                gCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCa                                         <  1:469867/93‑1 (MQ=255)

                                                 cATGCTACTACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCaa                                        <  1:4740355/93‑1 (MQ=255)

                                                  aTGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAg                                       <  1:3392406/93‑1 (MQ=255)

                                                   tGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGc                                      >  1:1958613/1‑93 (MQ=255)

                                                   tGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGc                                      >  1:3307267/1‑93 (MQ=255)

                                                   tGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGc                                      >  1:3627227/1‑93 (MQ=255)

                                                    gCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGcc                                     <  1:2826753/93‑1 (MQ=255)

                                                     cTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCa                                    <  1:2064839/93‑1 (MQ=255)

                                                      tACCGCTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   >  2:3028449/1‑93 (MQ=255)

                                                      tACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   <  2:1907354/93‑1 (MQ=255)

                                                      tACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   <  2:3321947/93‑1 (MQ=255)

                                                       aCCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAAt                                  <  2:4621763/93‑1 (MQ=255)

                                                         cACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATgg                                >  1:4378410/1‑93 (MQ=255)

                                                          aCTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGGGATTTGAGCAGGTAACGAATTCAAGCCAATGGa                               >  1:1521775/1‑93 (MQ=255)

                                                            tGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGaaa                             <  1:2261840/93‑1 (MQ=255)

                                                             gCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAAt                            <  1:2432756/93‑1 (MQ=255)

                                                               gAATTTAACCACTTACTGACAGGGCTCGCGATGTCGCACATTGCTTCGGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATaa                          <  1:2447194/93‑1 (MQ=255)

                                                                 aTTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACa                        >  1:2563795/1‑93 (MQ=255)

                                                                 aTTTAACCAC‑TACTGACATGGCTCGCGATGTCGCCCCATTCTTCTTGGAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATaaaca                       >  2:2320121/1‑90 (MQ=255)

                                                                    tAACCACTTACTGACTTAGATCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATaaaaaaa                     <  2:4217897/93‑2 (MQ=255)

                                                                     aaCCCCTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATa                    <  2:1508958/93‑1 (MQ=255)

                                                                     aaCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATa                    <  2:4743868/93‑1 (MQ=255)

                                                                     aaCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATa                    <  1:3959390/93‑1 (MQ=255)

                                                                     aaCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATa                    <  2:1377962/93‑1 (MQ=255)

                                                                     aaCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATa                    >  2:1152601/1‑93 (MQ=255)

                                                                      aCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAg                   >  2:354604/1‑93 (MQ=255)

                                                                       ccACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGt                  <  1:2092883/93‑1 (MQ=255)

                                                                        cACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGtt                 <  1:4164272/93‑1 (MQ=255)

                                                                         aCTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTGAAGCCAATGGAAATAACAAATAGTTc                >  1:1342947/1‑93 (MQ=255)

                                                                         aCTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTc                <  1:4465057/93‑1 (MQ=255)

                                                                         aCTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAAAAAATAGTTc                >  2:856049/1‑93 (MQ=255)

                                                                          cTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCg               >  1:827317/1‑93 (MQ=255)

                                                                           ttACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGc              >  2:532122/1‑93 (MQ=255)

                                                                           ttACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGc              <  1:3693270/93‑1 (MQ=255)

                                                                            tACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTTAAGCCAATGGAAATAACAAATAGTTCGCt             >  2:1116387/1‑93 (MQ=255)

                                                                            tACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCt             <  2:1870647/93‑1 (MQ=255)

                                                                            tACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCt             >  2:4029508/1‑93 (MQ=255)

                                                                             aCTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTc            <  2:2586241/93‑1 (MQ=255)

                                                                             aCTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTc            <  2:369659/93‑1 (MQ=255)

                                                                               tGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTCACGAATTCAAGCAAATGGAAATAACAAATAGTTCGCTCAt          >  1:4367011/1‑93 (MQ=255)

                                                                               tGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAt          <  1:349329/93‑1 (MQ=255)

                                                                               tGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAt          >  1:42985/1‑93 (MQ=255)

                                                                               tGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAt          <  2:2758997/93‑1 (MQ=255)

                                                                                gACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATg         >  1:4411205/1‑93 (MQ=255)

                                                                                 aCATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGa        >  2:3802963/1‑93 (MQ=255)

                                                                                 aCATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGa        >  2:1283105/1‑93 (MQ=255)

                                                                                  cATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGAc       <  1:481025/93‑1 (MQ=255)

                                                                                  cATGCCTCGCGATGTCGCACATTGCTTCTGAAAAGTGATTTGAGCAGTTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGAc       <  2:692834/93‑1 (MQ=255)

                                                                                   aTGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACt      >  1:2641963/1‑93 (MQ=255)

                                                                                    tGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTa     <  1:1584579/93‑1 (MQ=255)

                                                                                    tGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTa     >  2:2238279/1‑93 (MQ=255)

                                                                                    tGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTa     >  1:4193354/1‑93 (MQ=255)

                                                                                     ggCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAt    >  2:1969534/1‑93 (MQ=255)

AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTT  >  NC_000913/4227705‑4227884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT  >  NC_000913/4227702‑4227882

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCG                                                                                         >  SRR4302544.3205377/1‑94 (MQ=60)

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCG                                                                                         <  SRR4302544.4042498/94‑1 (MQ=60)

 TCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGC                                                                                        >  SRR4302544.2920832/1‑94 (MQ=60)

   AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGA                                                                                      >  SRR4302544.2631782/1‑94 (MQ=60)

    ATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAT                                                                                     <  SRR4302544.1126508/94‑1 (MQ=60)

      AAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGT                                                                                   >  SRR4302544.2952502/1‑94 (MQ=60)

      AAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGT                                                                                   >  SRR4302544.629012/1‑94 (MQ=60)

      AAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGT                                                                                   <  SRR4302544.3808109/94‑1 (MQ=60)

      AAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGT                                                                                   >  SRR4302544.4176938/1‑94 (MQ=60)

        AAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGGTCGCGATGTCG                                                                                 <  SRR4302544.2789237/94‑1 (MQ=60)

        AAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCG                                                                                 <  SRR4302544.4206526/94‑1 (MQ=60)

        AAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCG                                                                                 >  SRR4302544.2179788/1‑94 (MQ=60)

         AGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGC                                                                                <  SRR4302544.3060640/94‑1 (MQ=60)

           CAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTGCTGACATGGCTCGCGATGGCGCAA                                                                              >  SRR4302544.3804792/1‑94 (MQ=60)

           CAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCAC                                                                              <  SRR4302544.74909/94‑1 (MQ=60)

             GTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACAT                                                                            <  SRR4302544.2348943/94‑1 (MQ=60)

              TTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATT                                                                           <  SRR4302544.724118/94‑1 (MQ=60)

              TTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATT                                                                           <  SRR4302544.1670015/94‑1 (MQ=60)

              TTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATT                                                                           <  SRR4302544.2417015/94‑1 (MQ=60)

               TCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTC                                                                          >  SRR4302544.3090933/1‑94 (MQ=60)

                CCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGC                                                                         >  SRR4302544.2803645/1‑94 (MQ=60)

                CCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGC                                                                         >  SRR4302544.2675827/1‑94 (MQ=60)

                  ATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCAAATTGCTT                                                                       >  SRR4302544.4551779/1‑94 (MQ=60)

                   TTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTC                                                                      >  SRR4302544.135940/1‑94 (MQ=60)

                       TTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGA                                                                  >  SRR4302544.1763396/1‑94 (MQ=60)

                       TTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGA                                                                  >  SRR4302544.1141055/1‑94 (MQ=60)

                       TTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGA                                                                  >  SRR4302544.2297511/1‑94 (MQ=60)

                        TCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAA                                                                 >  SRR4302544.1057360/1‑94 (MQ=60)

                        TCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAA                                                                 >  SRR4302544.3453176/1‑94 (MQ=60)

                        TCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAA                                                                 >  SRR4302544.2640312/1‑94 (MQ=60)

                         CTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAA                                                                >  SRR4302544.3705630/1‑94 (MQ=60)

                         CTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATCTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAA                                                                <  SRR4302544.2725362/94‑1 (MQ=60)

                         CTCCGGTTAATAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAA                                                                <  SRR4302544.2725361/94‑1 (MQ=60)

                          TCCGGTTAGTAAGAAATCACGGGNGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAG                                                               <  SRR4302544.2042396/94‑1 (MQ=60)

                           CCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGTCTCGCGATGTCGCACATTGCTgttggaacgt                                                              >  SRR4302544.4428648/1‑84 (MQ=60)

                            CGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTG                                                             >  SRR4302544.289977/1‑94 (MQ=60)

                            CGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTG                                                             <  SRR4302544.2581438/94‑1 (MQ=60)

                             GGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTTGGAAGTGA                                                            >  SRR4302544.1712507/1‑94 (MQ=60)

                             GGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGA                                                            >  SRR4302544.4457369/1‑94 (MQ=60)

                             GGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGA                                                            <  SRR4302544.322799/94‑1 (MQ=60)

                              GTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAT                                                           <  SRR4302544.416371/94‑1 (MQ=60)

                              GTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGAGATGTCGCACATTGCTTCTGGAAAGTGAT                                                           <  SRR4302544.4009959/94‑1 (MQ=60)

                               TTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGCTGTCGCACATTGCTTCTGGGAAGAGATT                                                          >  SRR4302544.2020092/1‑94 (MQ=60)

                                TAGTAAGAAATCACGGGAGGCATGCTACCCCTGCGCCTTTAACCACTTACTGACATGGCTCGCTATGTCGACCACTGCTTCTGGAAAagccatg                                                         >  SRR4302544.2742533/1‑87 (MQ=52)

                                TAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTT                                                         <  SRR4302544.484481/94‑1 (MQ=60)

                                TAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTT                                                         >  SRR4302544.2742532/1‑94 (MQ=60)

                                 AGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTG                                                        >  SRR4302544.4632646/1‑94 (MQ=60)

                                 AGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTG                                                        >  SRR4302544.4347285/1‑94 (MQ=60)

                                  GTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAAGGATTTGA                                                       >  SRR4302544.1158551/1‑94 (MQ=60)

                                  ATAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGA                                                       <  SRR4302544.3501604/94‑1 (MQ=60)

                                   TAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAG                                                      <  SRR4302544.2712566/94‑1 (MQ=60)

                                   TAAAAAATCACGGGAGGCATCCTACCACTGCGAATTTAAACACTTACTGACATGGCTCGAGATGTCGCACATTGCTTCTGGAAAGTGATTTGAG                                                      <  SRR4302544.591037/94‑1 (MQ=60)

                                    AAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGC                                                     >  SRR4302544.2404759/1‑94 (MQ=60)

                                      GAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAG                                                   >  SRR4302544.2888951/1‑94 (MQ=60)

                                      GAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAG                                                   <  SRR4302544.2926539/94‑1 (MQ=60)

                                       AAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGG                                                  <  SRR4302544.4337566/94‑1 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                <  SRR4302544.867818/94‑1 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                <  SRR4302544.3248964/94‑1 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                <  SRR4302544.3579148/94‑1 (MQ=60)

                                          ACACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAA                                               <  SRR4302544.1653479/94‑1 (MQ=60)

                                           CACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGGCATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAAC                                              <  SRR4302544.4011121/94‑1 (MQ=60)

                                            ACGTGAGGCATGCTACCACTGCGAATTGAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACG                                             <  SRR4302544.179159/94‑1 (MQ=60)

                                            ACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACG                                             <  SRR4302544.2909692/94‑1 (MQ=60)

                                            ACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACG                                             <  SRR4302544.179158/94‑1 (MQ=60)

                                            ACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACA                                             >  SRR4302544.3012739/1‑94 (MQ=60)

                                              GGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAA                                           >  SRR4302544.3131229/1‑94 (MQ=60)

                                                 AGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTC                                        <  SRR4302544.4491269/94‑1 (MQ=60)

                                                 AGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTC                                        >  SRR4302544.1035543/1‑94 (MQ=60)

                                                 AGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTC                                        <  SRR4302544.1938742/94‑1 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                      <  SRR4302544.474308/94‑1 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                      <  SRR4302544.4065937/94‑1 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                      <  SRR4302544.474462/94‑1 (MQ=60)

                                                    CATGCTACTACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                     <  SRR4302544.4787076/94‑1 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                     <  SRR4302544.4427942/94‑1 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                     <  SRR4302544.2102806/94‑1 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                     <  SRR4302544.4331658/94‑1 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGCGATTTGAGCAGGTAACGCATTCAAGC                                    >  SRR4302544.520455/1‑94 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                    >  SRR4302544.3663362/1‑94 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                    >  SRR4302544.1977616/1‑94 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                    <  SRR4302544.3424838/94‑1 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                    >  SRR4302544.3338762/1‑94 (MQ=60)

                                                      TGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCC                                   >  SRR4302544.1356486/1‑94 (MQ=60)

                                                       GCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCA                                  <  SRR4302544.2854067/94‑1 (MQ=60)

                                                        CTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAA                                 <  SRR4302544.2084683/94‑1 (MQ=60)

                                                        CTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGGGATTTGAGCAGGTAACGAATTCAAGCCAA                                 >  SRR4302544.2737316/1‑94 (MQ=60)

                                                         TACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAAT                                <  SRR4302544.375307/94‑1 (MQ=60)

                                                           CCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGG                              >  SRR4302544.4422775/1‑94 (MQ=60)

                                                            CACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCGCATTGCTTCTTGAAAGTGATTTGAGCATGTAACGAAATTAAGCCAATGGA                             >  SRR4302544.3431465/1‑94 (MQ=60)

                                                            CACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGGGATTTGAGCAGGTAACGAATTCAAGCCAATGGA                             >  SRR4302544.1536877/1‑94 (MQ=60)

                                                             ACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAA                            <  SRR4302544.846278/94‑1 (MQ=60)

                                                             ACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAA                            >  SRR4302544.2747374/1‑94 (MQ=60)

                                                               TGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAAT                          <  SRR4302544.2283118/94‑1 (MQ=60)

                                                               TGCGAATTTAACCACTTACTGACATGGCGCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAAT                          <  SRR4302544.994778/94‑1 (MQ=60)

                                                                GCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATA                         <  SRR4302544.2455934/94‑1 (MQ=60)

                                                                 CGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAA                        >  SRR4302544.2741256/1‑94 (MQ=60)

                                                                  GAATTTAACCACTTACTGACAGGGCTCGCGATGTCGCACATTGCTTCGGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAAC                       <  SRR4302544.2470527/94‑1 (MQ=60)

                                                                   AATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACA                      >  SRR4302544.2588426/1‑94 (MQ=60)

                                                                      TTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAAT                   <  SRR4302544.3326056/94‑1 (MQ=60)

                                                                       TAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATA                  <  SRR4302544.718108/94‑1 (MQ=60)

                                                                        AACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAG                 <  SRR4302544.4052457/94‑1 (MQ=60)

                                                                        AACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAG                 <  SRR4302544.3998766/94‑1 (MQ=60)

                                                                          CCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTT               <  SRR4302544.2113026/94‑1 (MQ=60)

                                                                          CCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTT               >  SRR4302544.4556272/1‑94 (MQ=60)

                                                                          CCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGGTATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTT               <  SRR4302544.2464493/94‑1 (MQ=60)

                                                                           CACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTGAAGCCAATGGAAATAACAAATAGTTC              >  SRR4302544.1356232/1‑94 (MQ=60)

                                                                           CACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTC              <  SRR4302544.4205523/94‑1 (MQ=60)

                                                                            acgttctgcCATGGCTCGCGATGTCCCAATTTGCTTCGGGAAGGTGATTTGAGCAGGTAACAAATACAAGCCAATGGAAATAACAAATAGTTCG             <  SRR4302544.2671209/85‑1 (MQ=48)

                                                                            CCTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCG             <  SRR4302544.4750154/94‑1 (MQ=60)

                                                                            ACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCG             <  SRR4302544.4509984/94‑1 (MQ=60)

                                                                            ACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCG             >  SRR4302544.835436/1‑94 (MQ=60)

                                                                            ACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCG             <  SRR4302544.622890/94‑1 (MQ=60)

                                                                             CTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGC            >  SRR4302544.894040/1‑94 (MQ=60)

                                                                             CTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGC            >  SRR4302544.776173/1‑94 (MQ=60)

                                                                             CTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGC            <  SRR4302544.3911500/94‑1 (MQ=60)

                                                                              TTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTTAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCT           >  SRR4302544.4790308/1‑94 (MQ=60)

                                                                              TTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCT           >  SRR4302544.2993723/1‑94 (MQ=60)

                                                                              TTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCT           <  SRR4302544.3730067/94‑1 (MQ=60)

                                                                                ACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCA         <  SRR4302544.1540195/94‑1 (MQ=60)

                                                                                ACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCA         >  SRR4302544.338132/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTCACGAATTCAAGCAAATGGAAATAACAAATAGTTCGCTCAT        >  SRR4302544.4411303/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAT        >  SRR4302544.1296574/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAT        >  SRR4302544.4411304/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAT        >  SRR4302544.43408/1‑94 (MQ=60)

                                                                                  TGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATG       <  SRR4302544.352913/94‑1 (MQ=60)

                                                                                  TGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATG       >  SRR4302544.452981/1‑94 (MQ=60)

                                                                                  TGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATG       >  SRR4302544.4455744/1‑94 (MQ=60)

                                                                                     CATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACT    >  SRR4302544.2667290/1‑94 (MQ=60)

                                                                                     CATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACT    <  SRR4302544.485733/94‑1 (MQ=60)

                                                                                      ATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTA   >  SRR4302544.1708238/1‑94 (MQ=60)

                                                                                      ATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTA   >  SRR4302544.4234888/1‑94 (MQ=60)

                                                                                       TGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT  <  SRR4302544.459445/94‑1 (MQ=60)

                                                                                       TGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT  <  SRR4302544.1600229/94‑1 (MQ=60)

                                                                                       TGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT  <  SRR4302544.4491848/94‑1 (MQ=60)

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT  >  NC_000913/4227702‑4227882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`