Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,261,143 T→C E223G (GAA→GGA)  qor ← quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,261,1430TC98.6% 457.4 / ‑2.5 146E223G (GAA→GGA) qorquinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (86/58);  total (88/58)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01

Only 100 of 160 total aligned reads displayed.

AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTC  >  NC_000913/4261053‑4261231
                                                                                          |                                                                                        
agaATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTccc                                                                                        >  2:1810949/1‑93 (MQ=255)
agaATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTccc                                                                                        <  1:3784978/93‑1 (MQ=255)
 gaATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTcccc                                                                                       <  2:2810860/93‑1 (MQ=255)
 gaATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTcccc                                                                                       >  1:3340514/1‑93 (MQ=255)
 gaATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTcccc                                                                                       >  1:3807129/1‑93 (MQ=255)
   aTGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAg                                                                                     >  2:1984529/1‑93 (MQ=255)
   aTGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAg                                                                                     >  1:4565795/1‑93 (MQ=255)
    tGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGAAGGCAATCCAGCGACCGTCCCCAgg                                                                                    >  1:4045423/1‑93 (MQ=255)
      ccTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGgtg                                                                                  >  1:1543763/1‑93 (MQ=255)
      ccTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGgtg                                                                                  >  1:2182957/1‑93 (MQ=255)
       cTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGgtgt                                                                                 >  2:1706262/1‑93 (MQ=255)
        tAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGtc                                                                                <  1:4414774/93‑1 (MQ=255)
        tAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGtc                                                                                >  1:1133647/1‑93 (MQ=255)
         aaGTTCACACCGGTAACCGCACCTGATGAGTTGCTAAAACTGACCATTACGCAGCGGCGTTGCGGGCGGTCCAGCGACCGTCCCCAGGTGtct                                                                               >  2:3788109/1‑93 (MQ=255)
         aaGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGtct                                                                               <  2:1884850/93‑1 (MQ=255)
          aGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGtctc                                                                              >  1:1993126/1‑93 (MQ=255)
          aGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGtctc                                                                              <  1:652116/93‑1 (MQ=255)
           gTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTctct                                                                             <  1:367474/93‑1 (MQ=255)
           gTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGAGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTctct                                                                             >  2:4405201/1‑93 (MQ=255)
            ttCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTg                                                                            >  2:104271/1‑93 (MQ=255)
             tCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGc                                                                           >  1:2154175/1‑93 (MQ=255)
              cacaCCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGcc                                                                          >  1:4157454/1‑93 (MQ=255)
               acacCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGccc                                                                         >  2:4478344/1‑93 (MQ=255)
                 acCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCAc                                                                       <  1:985527/93‑1 (MQ=255)
                 acCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCAc                                                                       >  2:3924141/1‑93 (MQ=255)
                  ccGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACg                                                                      <  1:1572131/93‑1 (MQ=255)
                  ccGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACg                                                                      >  2:3023604/1‑93 (MQ=255)
                  ccGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACg                                                                      >  1:1289816/1‑93 (MQ=255)
                   cGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACgg                                                                     >  2:1816906/1‑93 (MQ=255)
                    ggTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGa                                                                    >  1:1423797/1‑93 (MQ=255)
                     gTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGaa                                                                   >  2:2334959/1‑93 (MQ=255)
                      tAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAAt                                                                  <  2:4487278/93‑1 (MQ=255)
                      tAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAAt                                                                  <  2:2889691/93‑1 (MQ=255)
                       aaCCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATc                                                                 >  2:4157962/1‑93 (MQ=255)
                        aCCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCg                                                                >  2:1202747/1‑93 (MQ=255)
                         ccGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGt                                                               >  2:4327876/1‑93 (MQ=255)
                          cGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCgca                                                              >  1:344995/1‑91 (MQ=255)
                            cACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTaca                                                            >  2:3492751/1‑93 (MQ=255)
                              ccTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTAcacc                                                          >  2:1408003/1‑93 (MQ=255)
                               cTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTAcacca                                                         >  2:1400241/1‑93 (MQ=255)
                               cTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTAcacca                                                         <  2:2285542/93‑1 (MQ=255)
                                 gatgaGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCAcg                                                       >  1:76566/1‑93 (MQ=255)
                                   tgaGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCAAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCAcgcg                                                     >  2:2890782/1‑93 (MQ=255)
                                      gTTGCCAAAACTGATCATTACGCCGCGGCGTTGCAGGCAATACAGCGACCGTCCACAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCAc                                                  >  2:4636974/1‑93 (MQ=255)
                                      gTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCATGCGCAc                                                  >  2:2153388/1‑93 (MQ=255)
                                          ccAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTc                                              <  1:2466899/93‑1 (MQ=255)
                                           cAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCt                                             <  1:3266754/93‑1 (MQ=255)
                                           cAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCt                                             <  1:2590072/93‑1 (MQ=255)
                                            aaaaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCtt                                            >  2:1232953/1‑93 (MQ=255)
                                             aaaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTa                                           >  2:1886319/1‑93 (MQ=255)
                                              aaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTAc                                          <  1:2231419/93‑1 (MQ=255)
                                               aCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTAcc                                         >  1:1065286/1‑93 (MQ=255)
                                               aCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTAcc                                         <  1:4384093/93‑1 (MQ=255)
                                                cTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTAccg                                        >  1:2614732/1‑93 (MQ=255)
                                                     cATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGt                                   >  2:2783659/1‑93 (MQ=255)
                                                     cATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGt                                   <  2:1730508/93‑1 (MQ=255)
                                                      aTTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTg                                  >  1:3750051/1‑93 (MQ=255)
                                                       ttAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGa                                 >  1:2962845/1‑93 (MQ=255)
                                                       ttAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGa                                 <  2:3576097/93‑1 (MQ=255)
                                                       agaaGCCGTGGCGTTGCGGGCAATCCAGCGACCGTCCCCAGGTGTCTCTCCCCACGAAAACGTACACCACGCGCACTTTCTTTCCGCCGGTGa                                 <  2:3576098/91‑1 (MQ=255)
                                                        tAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGAt                                >  2:3890146/1‑93 (MQ=255)
                                                         acgCCGCGGCGTTGCACGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGAtc                               <  2:3872375/91‑1 (MQ=255)
                                                         aaGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGAtc                               >  1:1998315/1‑93 (MQ=255)
                                                          aGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGAtct                              <  2:4679809/93‑1 (MQ=255)
                                                             cgcgGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCtt                           >  1:4048925/1‑93 (MQ=255)
                                                             cgcgGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCtt                           >  2:641681/1‑93 (MQ=255)
                                                             cgcgGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCtt                           >  2:2028840/1‑93 (MQ=255)
                                                                 gcgTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTaa                       >  2:4520186/1‑93 (MQ=255)
                                                                 gcgTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTac                       >  1:3661233/1‑92 (MQ=255)
                                                                 gcgTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTaa                       >  2:1761766/1‑93 (MQ=255)
                                                                 gcgTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTaa                       >  1:4337130/1‑93 (MQ=255)
                                                                 gcgTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTaa                       <  1:4599390/93‑1 (MQ=255)
                                                                  cgTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAAc                      <  2:1327894/93‑1 (MQ=255)
                                                                   gTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAAcc                     >  1:1085796/1‑93 (MQ=255)
                                                                    ttGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCg                    >  2:3637838/1‑93 (MQ=255)
                                                                     tGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGc                   <  1:1726355/93‑1 (MQ=255)
                                                                       cAGGCAATCCAGCTACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTc                 <  2:736111/93‑1 (MQ=255)
                                                                       cAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTc                 <  1:611465/93‑1 (MQ=255)
                                                                        aGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCg                >  1:415457/1‑93 (MQ=255)
                                                                        aGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCg                >  2:3861640/1‑93 (MQ=255)
                                                                            aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCa            >  1:1577191/1‑93 (MQ=255)
                                                                            aaTCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCa            <  1:3415305/93‑1 (MQ=255)
                                                                            aaTCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCa            <  1:3283425/93‑1 (MQ=255)
                                                                             aTCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAg           >  2:1325930/1‑93 (MQ=255)
                                                                             aTCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAg           <  1:1914770/93‑1 (MQ=255)
                                                                              tCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGa          >  2:538650/1‑93 (MQ=255)
                                                                              tCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGa          <  2:2814785/93‑1 (MQ=255)
                                                                              tCCAGCGACCGTCCCCAGGAGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTTACCACTCGACCAGa          >  1:1695203/1‑93 (MQ=255)
                                                                               ccAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAt         <  1:704163/93‑1 (MQ=255)
                                                                                cAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATc        >  1:3077458/1‑93 (MQ=255)
                                                                                 aGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc       >  1:2340626/1‑93 (MQ=255)
                                                                                  gCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCct      >  2:127713/1‑93 (MQ=255)
                                                                                  gCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCct      >  2:3323780/1‑93 (MQ=255)
                                                                                  gCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCct      <  1:758519/93‑1 (MQ=255)
                                                                                   cGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCctc     >  1:2939338/1‑93 (MQ=255)
                                                                                    gACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCctct    >  2:3695249/1‑93 (MQ=255)
                                                                                    gACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCctct    <  2:1470116/93‑1 (MQ=255)
                                                                                     aCCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATACTCtt   >  2:1055851/1‑93 (MQ=255)
                                                                                      ccGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTc  <  1:2317714/93‑1 (MQ=255)
                                                                                      ccGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTc  >  1:1323439/1‑93 (MQ=255)
                                                                                          |                                                                                        
AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTC  >  NC_000913/4261053‑4261231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACG  >  NC_000913/4261053‑4261234
                                                                                          |                                                                                           
AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCC                                                                                          >  SRR4302544.3372357/1‑94 (MQ=60)
AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCC                                                                                          >  SRR4302544.3844859/1‑94 (MQ=60)
AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCC                                                                                          <  SRR4302544.3822453/94‑1 (MQ=60)
AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCC                                                                                          >  SRR4302544.3844858/1‑94 (MQ=60)
 GAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCA                                                                                         <  SRR4302544.508765/94‑1 (MQ=60)
  AATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAG                                                                                        >  SRR4302544.4611314/1‑94 (MQ=60)
   ATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGAAGGCAATCCAGCGACCGTCCCCAGG                                                                                       >  SRR4302544.4085718/1‑94 (MQ=60)
     GCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTG                                                                                     >  SRR4302544.2203618/1‑94 (MQ=60)
     GCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTG                                                                                     >  SRR4302544.1559130/1‑94 (MQ=60)
      CCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGTAGGCAATCCAGCGACCGTCCCCAGGTGT                                                                                    >  SRR4302544.3364109/1‑94 (MQ=60)
       CTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACNATTAAGCCGCGGCGTTGCAGGNANNNNAGNGACCGTCNCNAGGTNNN                                                                                   >  SRR4302544.1498690/1‑94 (MQ=60)
       CTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTC                                                                                   >  SRR4302544.1144932/1‑94 (MQ=60)
        TAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCT                                                                                  <  SRR4302544.4459345/94‑1 (MQ=60)
         AAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTC                                                                                 >  SRR4302544.2012477/1‑94 (MQ=60)
          AGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCT                                                                                <  SRR4302544.658573/94‑1 (MQ=60)
           GTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTG                                                                               <  SRR4302544.371232/94‑1 (MQ=60)
            TTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGC                                                                              >  SRR4302544.2174660/1‑94 (MQ=60)
             TCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCC                                                                             >  SRR4302544.4198633/1‑94 (MQ=60)
                 ACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACG                                                                         <  SRR4302544.995191/94‑1 (MQ=60)
                 ACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACG                                                                         >  SRR4302544.1132417/1‑94 (MQ=60)
                 ACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACG                                                                         >  SRR4302544.1302514/1‑94 (MQ=60)
                  CCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGG                                                                        <  SRR4302544.1587678/94‑1 (MQ=60)
                   CGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGA                                                                       >  SRR4302544.1437966/1‑94 (MQ=60)
                      TAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATC                                                                    <  SRR4302544.864545/94‑1 (MQ=60)
                       AACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCG                                                                   <  SRR4302544.1978266/94‑1 (MQ=60)
                         CCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGCA                                                                 >  SRR4302544.348551/1‑94 (MQ=60)
                               CTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCAC                                                           <  SRR4302544.4495664/94‑1 (MQ=60)
                               CTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCAC                                                           >  SRR4302544.2612540/1‑94 (MQ=60)
                                TGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACG                                                          >  SRR4302544.77306/1‑94 (MQ=60)
                                TGATGAGTTGCCAAAACTGACCATAAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACC                                                          >  SRR4302544.3271994/1‑94 (MQ=60)
                                  ATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCG                                                        >  SRR4302544.131505/1‑94 (MQ=60)
                                    GAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGCATCGTACACCACGCGCA                                                      >  SRR4302544.218716/1‑94 (MQ=60)
                                     AGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCAC                                                     >  SRR4302544.1675434/1‑94 (MQ=60)
                                          CCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCT                                                <  SRR4302544.2490467/94‑1 (MQ=60)
                                           CAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTT                                               <  SRR4302544.2614991/94‑1 (MQ=60)
                                           CAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTT                                               <  SRR4302544.3297933/94‑1 (MQ=60)
                                              AACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACC                                            >  SRR4302544.1075876/1‑94 (MQ=60)
                                              AACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACC                                            <  SRR4302544.2252404/94‑1 (MQ=60)
                                               ACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCG                                           <  SRR4302544.4428488/94‑1 (MQ=60)
                                               ACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCG                                           >  SRR4302544.2639835/1‑94 (MQ=60)
                                                     CATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTG                                     <  SRR4302544.1738705/94‑1 (MQ=60)
                                                     CATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTG                                     >  SRR4302544.3787297/1‑94 (MQ=60)
                                                      ATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGA                                    >  SRR4302544.2991281/1‑94 (MQ=60)
                                                        TAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATC                                  >  SRR4302544.2017718/1‑94 (MQ=60)
                                                          AGCCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGGGATCTC                                >  SRR4302544.3648322/1‑94 (MQ=60)
                                                            CCGCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTT                              >  SRR4302544.4089253/1‑94 (MQ=60)
                                                             CGCGGCGTTGCAGGCAATGCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTT                             >  SRR4302544.430741/1‑94 (MQ=60)
                                                              GCGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTT                            <  SRR4302544.3009728/94‑1 (MQ=60)
                                                               CGGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTA                           >  SRR4302544.1432675/1‑94 (MQ=60)
                                                                GGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAC                          >  SRR4302544.3697753/1‑94 (MQ=60)
                                                                GGCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAA                          >  SRR4302544.4381269/1‑94 (MQ=60)
                                                                 GCGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAAC                         <  SRR4302544.4645119/94‑1 (MQ=60)
                                                                  CGTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACC                        >  SRR4302544.1096600/1‑94 (MQ=60)
                                                                   GTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCG                       >  SRR4302544.3455545/1‑94 (MQ=60)
                                                                   GTTGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCG                       >  SRR4302544.228779/1‑94 (MQ=60)
                                                                     TGCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCT                     <  SRR4302544.1743289/94‑1 (MQ=60)
                                                                      GCAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTC                    <  SRR4302544.2461520/94‑1 (MQ=60)
                                                                       GAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCG                   <  SRR4302544.4090154/94‑1 (MQ=60)
                                                                       CAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCG                   <  SRR4302544.617467/94‑1 (MQ=60)
                                                                       CAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCG                   <  SRR4302544.617613/94‑1 (MQ=60)
                                                                       CAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCG                   >  SRR4302544.727525/1‑94 (MQ=60)
                                                                       CAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCG                   >  SRR4302544.419699/1‑94 (MQ=60)
                                                                       CAGGCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCG                   <  SRR4302544.2761684/94‑1 (MQ=60)
                                                                          GCAATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACC                <  SRR4302544.4033710/94‑1 (MQ=60)
                                                                           CAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCA               >  SRR4302544.1592780/1‑94 (MQ=60)
                                                                            AATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAG              <  SRR4302544.3447947/94‑1 (MQ=60)
                                                                            AATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAG              <  SRR4302544.3314711/94‑1 (MQ=60)
                                                                             ATCCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGA             <  SRR4302544.1933276/94‑1 (MQ=60)
                                                                             ATCCAGCGACCGTCCCCAGGAGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTTACCACTCGACCAGA             >  SRR4302544.1711928/1‑94 (MQ=60)
                                                                               CCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATC           <  SRR4302544.711168/94‑1 (MQ=60)
                                                                               CCAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATC           >  SRR4302544.3106779/1‑94 (MQ=60)
                                                                                CAGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCC          >  SRR4302544.2362799/1‑94 (MQ=60)
                                                                                 AGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCT         >  SRR4302544.2038968/1‑94 (MQ=60)
                                                                                 AGCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCT         <  SRR4302544.3719202/94‑1 (MQ=60)
                                                                                  GCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTC        <  SRR4302544.765920/94‑1 (MQ=60)
                                                                                  GCGACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTC        >  SRR4302544.2967563/1‑94 (MQ=60)
                                                                                    GACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTT      >  SRR4302544.4405724/1‑94 (MQ=60)
                                                                                     ACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTC     >  SRR4302544.1569751/1‑94 (MQ=60)
                                                                                     ACCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTC     >  SRR4302544.1336457/1‑94 (MQ=60)
                                                                                      CCGTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCA    <  SRR4302544.2339654/94‑1 (MQ=60)
                                                                                        GTCCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACG  >  SRR4302544.1003598/1‑94 (MQ=60)
                                                                                          |                                                                                           
AGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACG  >  NC_000913/4261053‑4261234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑