Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,212 A→G R413R (CGT→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2120AG97.8% 550.1 / 0.3 180R413R (CGT→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (95/81);  total (97/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 192 total aligned reads displayed.

GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG  >  NC_000913/4270122‑4270301
                                                                                          |                                                                                         
 aagaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGgcgc                                                                                        >  2:3922286/1‑93 (MQ=255)
   gaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGt                                                                                      >  2:3857835/1‑93 (MQ=255)
    aaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTc                                                                                     <  2:3207212/93‑1 (MQ=255)
    aaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTc                                                                                     >  2:2213612/1‑93 (MQ=255)
     aTTCCATAGCATGACCAATGCTCATGCCGGAGATAGCAGGCAGCGGCGGATTCTCGACATAAACGGGGCGCGCTTCCCGACGCAGGCGCGTcc                                                                                    >  2:2669683/1‑93 (MQ=255)
       tCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCt                                                                                  <  1:2344538/93‑1 (MQ=255)
        ccATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCtt                                                                                 >  1:960423/1‑93 (MQ=255)
        ccATCGAATGACCAATGCGCATGGCGGATATAGCAGGCAGCGGCGTATTCTCGACACACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCtt                                                                                 <  2:956617/93‑1 (MQ=255)
         cATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTc                                                                                <  1:2802973/93‑1 (MQ=255)
          aTCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCg                                                                               >  2:2419880/1‑93 (MQ=255)
          aTCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCg                                                                               >  2:1025441/1‑93 (MQ=255)
          aTCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCg                                                                               >  1:3595912/1‑93 (MQ=255)
           tCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGc                                                                              >  1:4018271/1‑93 (MQ=255)
             gCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAg                                                                            <  1:3409210/93‑1 (MQ=255)
              cATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGc                                                                           >  1:991149/1‑93 (MQ=255)
              cATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGc                                                                           >  2:4485544/1‑93 (MQ=255)
              cATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGc                                                                           <  2:1231452/93‑1 (MQ=255)
               aTGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCt                                                                          <  2:2105529/93‑1 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                         >  2:803513/1‑93 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                         >  2:148584/1‑93 (MQ=255)
                  aCCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgc                                                                       >  1:1855354/1‑93 (MQ=255)
                   ccAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcg                                                                      >  1:2210644/1‑93 (MQ=255)
                    cAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgc                                                                     >  2:3307631/1‑93 (MQ=255)
                    cAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTAATCTCGACATACACGCGGCGCGCTTCCCGACGCAGGCGCGTCCATTCGCAGCTGtcgc                                                                     >  2:2524257/1‑93 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                    <  2:3069373/93‑2 (MQ=255)
                      aTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcgc                                                                   <  1:4689813/93‑3 (MQ=255)
                        gCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCgg                                                                 <  2:1100347/93‑1 (MQ=255)
                         cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                                <  2:598519/93‑1 (MQ=255)
                          tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                               >  2:3580094/1‑93 (MQ=255)
                            aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                             >  1:1191384/1‑93 (MQ=255)
                            aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                             <  1:4066265/93‑1 (MQ=255)
                               tCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                          <  2:3010315/93‑1 (MQ=255)
                               gcGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                          <  1:1177110/92‑1 (MQ=255)
                                   agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                      <  2:1390162/93‑1 (MQ=255)
                                    gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                     <  1:2561377/93‑1 (MQ=255)
                                    gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                     >  2:3702849/1‑93 (MQ=255)
                                    gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                     >  1:108089/1‑93 (MQ=255)
                                     aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                    <  1:2116609/93‑1 (MQ=255)
                                     aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                    >  1:1243085/1‑93 (MQ=255)
                                       aGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGCCGATTACTGATaaa                                                  >  2:1612869/1‑93 (MQ=255)
                                          aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                               <  1:3969172/93‑1 (MQ=255)
                                           ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                              >  2:4445850/1‑93 (MQ=255)
                                           ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                              >  1:1022293/1‑93 (MQ=255)
                                           ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                              <  1:626148/93‑1 (MQ=255)
                                            gcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTgg                                             >  2:3084914/1‑93 (MQ=255)
                                             cagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGc                                            <  2:1698079/93‑1 (MQ=255)
                                              agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCt                                           <  2:4559388/93‑1 (MQ=255)
                                              agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCt                                           >  1:2453305/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       >  1:4522475/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       >  1:4574253/1‑93 (MQ=255)
                                                   cgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttc                                      <  1:2403979/93‑1 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     >  2:2306917/1‑93 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     <  2:1892287/93‑1 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     >  1:720586/1‑93 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     <  2:2783434/93‑1 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                    >  1:1642800/1‑93 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                    >  1:2681842/1‑93 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                    >  2:4121284/1‑93 (MQ=255)
                                                      tttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  1:1027230/92‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  2:2868142/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   >  1:2055283/1‑93 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  1:3846572/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  2:559161/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   >  1:3143179/1‑93 (MQ=255)
                                                      aTTCTCGACATACACGTGGCACGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  1:4478819/93‑1 (MQ=255)
                                                       ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                  >  2:2040453/1‑93 (MQ=255)
                                                        tctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgc                                 >  2:4598449/1‑93 (MQ=255)
                                                        tctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgc                                 >  1:3774445/1‑93 (MQ=255)
                                                         ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                <  1:513906/93‑1 (MQ=255)
                                                         ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                >  1:1758133/1‑93 (MQ=255)
                                                         ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                >  2:3098006/1‑93 (MQ=255)
                                                           cGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTa                              >  2:741874/1‑93 (MQ=255)
                                                            gACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAc                             >  1:243071/1‑93 (MQ=255)
                                                            gACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAc                             >  1:819092/1‑93 (MQ=255)
                                                               aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGTGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                          <  2:4643022/93‑1 (MQ=255)
                                                               aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                          >  2:3803111/1‑93 (MQ=255)
                                                                tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                         >  2:2836247/1‑93 (MQ=255)
                                                                  cacGTGGCGCACTCCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCt                       <  2:1652029/93‑1 (MQ=255)
                                                                       ggCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAtt                  <  1:1490063/93‑1 (MQ=255)
                                                                         cgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTcc                <  2:396360/93‑1 (MQ=255)
                                                                           cgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGt              <  1:3076492/93‑1 (MQ=255)
                                                                           cgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGt              >  2:3485286/1‑93 (MQ=255)
                                                                             cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct            >  2:134195/1‑93 (MQ=255)
                                                                             cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct            >  1:3897183/1‑93 (MQ=255)
                                                                             cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct            >  1:3005771/1‑93 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc           <  1:3453720/93‑1 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc           <  1:18962/93‑1 (MQ=255)
                                                                               tCCGGACGCAGGCGCGTCGCTTCGCAGCTGGCGCGCGGACTATTACTGATAACCTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct          <  2:3246351/93‑1 (MQ=255)
                                                                                cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt         <  1:4322798/93‑1 (MQ=255)
                                                                                 ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt        <  2:2827242/93‑1 (MQ=255)
                                                                                  cGACGCAGGCGCGTCCCTTCGCAGCTTGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta       >  2:1177824/1‑93 (MQ=255)
                                                                                   gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat      <  2:1874222/93‑1 (MQ=255)
                                                                                   gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat      <  2:1537116/93‑1 (MQ=255)
                                                                                    gcGCAGGCACGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata     <  1:414188/92‑1 (MQ=255)
                                                                                    aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata     <  1:1303252/93‑1 (MQ=255)
                                                                                    aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata     >  2:179665/1‑93 (MQ=255)
                                                                                     cGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtatcg    >  2:1267440/1‑91 (MQ=255)
                                                                                      gCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgc   >  2:3291499/1‑93 (MQ=255)
                                                                                       cAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcg  >  1:3274436/1‑93 (MQ=255)
                                                                                       cAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcg  <  2:2488649/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG  >  NC_000913/4270122‑4270301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  >  NC_000913/4270119‑4270303
                                                                                             |                                                                                           
GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGG                                                                                             <  SRR4302544.3812979/94‑1 (MQ=60)
 TTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGC                                                                                            >  SRR4302544.2880883/1‑94 (MQ=60)
 TTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGC                                                                                            <  SRR4302544.3442151/94‑1 (MQ=60)
  TGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCG                                                                                           <  SRR4302544.2870479/94‑1 (MQ=60)
  TGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCAGACGCAGGCG                                                                                           >  SRR4302544.461282/1‑94 (MQ=60)
     AGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGT                                                                                        >  SRR4302544.1251015/1‑94 (MQ=60)
        ATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCC                                                                                     >  SRR4302544.2965757/1‑94 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   >  SRR4302544.969858/1‑94 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   <  SRR4302544.2366748/94‑1 (MQ=60)
            CATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCG                                                                                 >  SRR4302544.3630504/1‑94 (MQ=60)
            CATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCG                                                                                 <  SRR4302544.3976759/94‑1 (MQ=60)
            CATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCG                                                                                 <  SRR4302544.2829989/94‑1 (MQ=60)
             CTCGCATGACCAATGCTCATGTCGGATATAGCAGGCAGCTGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGC                                                                                <  SRR4302544.4034604/94‑1 (MQ=60)
             ATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGC                                                                                >  SRR4302544.4058244/1‑94 (MQ=60)
               CGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAG                                                                              >  SRR4302544.628575/1‑94 (MQ=60)
               CGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCACACGCAGGCGCGTCCCTTCGCAG                                                                              >  SRR4302544.2395400/1‑94 (MQ=60)
                GCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGC                                                                             <  SRR4302544.3922435/94‑1 (MQ=60)
                GCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGC                                                                             <  SRR4302544.3441803/94‑1 (MQ=60)
                GCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGC                                                                             >  SRR4302544.1000921/1‑94 (MQ=60)
                GCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTATCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCCGC                                                                             >  SRR4302544.1000918/1‑94 (MQ=60)
                 CATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCT                                                                            <  SRR4302544.1099586/94‑1 (MQ=60)
                    GACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGC                                                                         <  SRR4302544.1289413/94‑1 (MQ=60)
                    GACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGC                                                                         >  SRR4302544.1873296/1‑94 (MQ=60)
                     ACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCG                                                                        >  SRR4302544.2231449/1‑94 (MQ=60)
                      CCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGC                                                                       >  SRR4302544.2171570/1‑94 (MQ=60)
                      CCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGC                                                                       <  SRR4302544.1883089/94‑1 (MQ=60)
                        AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGC                                                                     >  SRR4302544.3597938/1‑94 (MQ=60)
                         ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                    >  SRR4302544.2018610/1‑94 (MQ=60)
                         ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                    <  SRR4302544.4736150/94‑1 (MQ=60)
                          TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                   >  SRR4302544.4634965/1‑94 (MQ=60)
                              CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGCCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACat                                                               >  SRR4302544.2313273/1‑92 (MQ=60)
                              CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGA                                                               >  SRR4302544.1203204/1‑94 (MQ=60)
                               ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                              <  SRR4302544.4106752/94‑1 (MQ=60)
                                  GCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAC                                                           <  SRR4302544.1188844/94‑1 (MQ=60)
                                      AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       >  SRR4302544.109074/1‑94 (MQ=60)
                                       GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                      >  SRR4302544.1255352/1‑94 (MQ=60)
                                       GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                      <  SRR4302544.2585981/94‑1 (MQ=60)
                                        ATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAA                                                     <  SRR4302544.2136940/94‑1 (MQ=60)
                                          AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                   >  SRR4302544.325071/1‑94 (MQ=60)
                                          AGCAAGCAGCGGCGTATTCTCCACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                   <  SRR4302544.1050036/94‑1 (MQ=60)
                                           GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                  >  SRR4302544.1415805/1‑94 (MQ=60)
                                            CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                 >  SRR4302544.632366/1‑94 (MQ=60)
                                             AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTG                                                <  SRR4302544.4008649/94‑1 (MQ=60)
                                             AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTG                                                >  SRR4302544.1032502/1‑94 (MQ=60)
                                              GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                               <  SRR4302544.632303/94‑1 (MQ=60)
                                              GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                               <  SRR4302544.1566166/94‑1 (MQ=60)
                                                CAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCT                                             >  SRR4302544.2476709/1‑94 (MQ=60)
                                                    GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                         >  SRR4302544.4567746/1‑94 (MQ=60)
                                                    GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                         >  SRR4302544.2437786/1‑94 (MQ=60)
                                                    GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                         >  SRR4302544.4619836/1‑94 (MQ=60)
                                                     GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                        <  SRR4302544.2472358/94‑1 (MQ=60)
                                                      CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                       <  SRR4302544.2426796/94‑1 (MQ=60)
                                                      CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                       >  SRR4302544.727691/1‑94 (MQ=60)
                                                       GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                      >  SRR4302544.1658997/1‑94 (MQ=60)
                                                       GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                      >  SRR4302544.2707541/1‑94 (MQ=60)
                                                        TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                     >  SRR4302544.2075060/1‑94 (MQ=60)
                                                        TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                     >  SRR4302544.3173060/1‑94 (MQ=60)
                                                        TATTCTCGACATACACGCGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                     <  SRR4302544.1201380/94‑1 (MQ=60)
                                                         TTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302544.1037475/94‑1 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302544.3884736/94‑1 (MQ=60)
                                                         ATTCTCGACATACACGTGGCACGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302544.4523844/94‑1 (MQ=60)
                                                          TTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGC                                   >  SRR4302544.3811852/1‑94 (MQ=60)
                                                           TCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCG                                  >  SRR4302544.1775338/1‑94 (MQ=60)
                                                           TCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCG                                  >  SRR4302544.1775337/1‑94 (MQ=60)
                                                            CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                 <  SRR4302544.518982/94‑1 (MQ=60)
                                                            CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                 <  SRR4302544.4060952/94‑1 (MQ=60)
                                                              CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                               >  SRR4302544.827094/1‑94 (MQ=60)
                                                              CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                               >  SRR4302544.245598/1‑94 (MQ=60)
                                                               GACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACC                              >  SRR4302544.4182079/1‑94 (MQ=60)
                                                                ACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCG                             <  SRR4302544.3165844/94‑1 (MQ=60)
                                                                  ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                           <  SRR4302544.3783655/94‑1 (MQ=60)
                                                                  ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                           >  SRR4302544.2497108/1‑94 (MQ=60)
                                                                  ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                           <  SRR4302544.4742062/94‑1 (MQ=60)
                                                                   TACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGC                          >  SRR4302544.186341/1‑94 (MQ=60)
                                                                     CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGCACCGCGCTG                        <  SRR4302544.4369731/94‑1 (MQ=60)
                                                                          GGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTC                   <  SRR4302544.1504794/94‑1 (MQ=60)
                                                                             GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                <  SRR4302544.3837438/94‑1 (MQ=60)
                                                                              CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC               <  SRR4302544.3105809/94‑1 (MQ=60)
                                                                               GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT              >  SRR4302544.3034587/1‑94 (MQ=60)
                                                                               GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT              >  SRR4302544.3034586/1‑94 (MQ=60)
                                                                               GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT              >  SRR4302544.3935889/1‑94 (MQ=60)
                                                                               GCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTAGCGTACCGCGCTGGATTCCGTCT              >  SRR4302544.742876/1‑94 (MQ=60)
                                                                                 TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT            <  SRR4302544.3486693/94‑1 (MQ=60)
                                                                                 TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT            <  SRR4302544.19152/94‑1 (MQ=60)
                                                                                   CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT          <  SRR4302544.4199942/94‑1 (MQ=60)
                                                                                   CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT          <  SRR4302544.4366825/94‑1 (MQ=60)
                                                                                   CCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT          >  SRR4302544.2795205/1‑94 (MQ=60)
                                                                                    CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA         >  SRR4302544.839575/1‑94 (MQ=60)
                                                                                     CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT        >  SRR4302544.346444/1‑94 (MQ=60)
                                                                                      GACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATA       >  SRR4302544.2615013/1‑94 (MQ=60)
                                                                                       ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG      <  SRR4302544.1316063/94‑1 (MQ=60)
                                                                                         GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG    >  SRR4302544.3305653/1‑94 (MQ=60)
                                                                                          CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG   >  SRR4302544.3209291/1‑94 (MQ=60)
                                                                                           AGGCGCGTCCCTTCGCAGCTCGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  <  SRR4302544.1455765/94‑1 (MQ=60)
                                                                                             |                                                                                           
GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  >  NC_000913/4270119‑4270303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑