Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,212 A→G R413R (CGT→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2120AG100.0% 816.2 / NA 255R413R (CGT→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (134/121);  total (134/121)

Only 100 of 265 total aligned reads displayed.

GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG  >  NC_000913/4270122‑4270301
                                                                                          |                                                                                         
gaagaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGcg                                                                                         <  2:2879701/93‑1 (MQ=255)
 aagaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGgcgc                                                                                        <  2:4214457/93‑1 (MQ=255)
 aagaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGgcgc                                                                                        >  2:3614327/1‑93 (MQ=255)
   gaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGt                                                                                      >  1:2610768/1‑93 (MQ=255)
   gaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGt                                                                                      >  2:2757802/1‑93 (MQ=255)
      ttCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTccc                                                                                   <  2:1314830/93‑1 (MQ=255)
      ttCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTccc                                                                                   >  2:3719886/1‑93 (MQ=255)
        ccATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCtt                                                                                 >  1:5586697/1‑93 (MQ=255)
         cATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCATATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTc                                                                                >  2:6496014/1‑93 (MQ=255)
           tCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGc                                                                              >  1:5081101/1‑93 (MQ=255)
             gCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAg                                                                            <  1:4812070/93‑1 (MQ=255)
             gCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAg                                                                            >  2:3644406/1‑93 (MQ=255)
             gCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAg                                                                            >  2:4258141/1‑93 (MQ=255)
               aTGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCt                                                                          >  2:5816318/1‑93 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                         >  1:1409487/1‑93 (MQ=255)
                  aCCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgc                                                                       >  2:1559219/1‑93 (MQ=255)
                   ccAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcg                                                                      >  1:5534252/1‑93 (MQ=255)
                    cAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgc                                                                     >  1:5765184/1‑93 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                    >  1:2904859/1‑92 (MQ=255)
                      aTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcgc                                                                   >  2:1906684/1‑91 (MQ=255)
                       tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                  >  2:3050328/1‑93 (MQ=255)
                        gCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCgg                                                                 <  2:76646/93‑1 (MQ=255)
                          tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                               >  1:5904599/1‑93 (MQ=255)
                          tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                               >  1:872674/1‑93 (MQ=255)
                          tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                               >  2:325138/1‑93 (MQ=255)
                             tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACCCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                            <  1:6165649/93‑1 (MQ=255)
                             tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                            >  1:6465833/1‑93 (MQ=255)
                             tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                            <  1:4610703/93‑1 (MQ=255)
                              gTCGTAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTGCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAtt                                                           <  2:2683078/93‑1 (MQ=255)
                               tCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                          >  2:1785363/1‑93 (MQ=255)
                               tCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                          >  1:3754263/1‑93 (MQ=255)
                                cGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTaa                                                         <  1:3481054/93‑2 (MQ=255)
                                cGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAc                                                         <  2:6615910/93‑1 (MQ=255)
                                cGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAc                                                         <  1:1934416/93‑1 (MQ=255)
                                 ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                        >  1:1091707/1‑93 (MQ=255)
                                 ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                        <  1:2474468/93‑1 (MQ=255)
                                 ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                        <  1:422255/93‑1 (MQ=255)
                                  gagaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTg                                                       <  1:1292186/93‑1 (MQ=255)
                                    gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                     <  2:5934562/93‑1 (MQ=255)
                                    gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                     <  1:3971335/93‑1 (MQ=255)
                                    gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                     >  1:3382798/1‑93 (MQ=255)
                                     aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                    >  2:2355687/1‑93 (MQ=255)
                                     aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                    <  2:4823603/93‑1 (MQ=255)
                                     aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                    <  2:6131164/93‑1 (MQ=255)
                                        gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                 >  2:5345014/1‑93 (MQ=255)
                                        gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                 <  2:4973092/93‑1 (MQ=255)
                                        gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                 >  2:3078674/1‑93 (MQ=255)
                                              agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCt                                           <  1:4445257/93‑1 (MQ=255)
                                              agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCt                                           <  1:2154703/93‑1 (MQ=255)
                                               gcggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTa                                          >  1:2860393/1‑93 (MQ=255)
                                                cggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTaa                                         <  2:3108679/93‑1 (MQ=255)
                                                cggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTaa                                         >  2:2107568/1‑93 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                        <  2:5036456/93‑1 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                        >  1:3919070/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       >  2:5496196/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       >  1:158739/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       >  2:4793657/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       <  2:3163287/93‑1 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     >  1:1041720/1‑93 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     <  2:5471319/93‑1 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     >  1:5263702/1‑93 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                    >  2:4348706/1‑93 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  2:2379372/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   >  1:756276/1‑93 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  1:1652381/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   >  2:1854546/1‑93 (MQ=255)
                                                       ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                  >  1:93903/1‑93 (MQ=255)
                                                       ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                  <  1:3208633/93‑1 (MQ=255)
                                                          tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                               >  2:5851416/1‑93 (MQ=255)
                                                             gcATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                            <  1:2091869/92‑1 (MQ=255)
                                                               aTACACGTGGCGCGCTTTCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGGTAATTCTTAGTGTACtgc                          >  2:2313942/1‑93 (MQ=255)
                                                               aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                          >  1:2704957/1‑93 (MQ=255)
                                                                taaccGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                         <  1:573269/89‑1 (MQ=255)
                                                                tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                         >  2:3926430/1‑93 (MQ=255)
                                                                  cacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCt                       >  1:1903831/1‑93 (MQ=255)
                                                                     gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGa                    <  1:1796472/93‑1 (MQ=255)
                                                                     gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTAGa                    >  2:6022496/1‑93 (MQ=255)
                                                                      tGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAt                   <  1:5217737/93‑1 (MQ=255)
                                                                      tGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAt                   <  2:2262961/93‑1 (MQ=255)
                                                                       ggCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAtt                  <  2:6233061/93‑1 (MQ=255)
                                                                        gcgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGTTTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTc                 <  2:5149557/93‑1 (MQ=255)
                                                                            gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc             <  2:461655/93‑1 (MQ=255)
                                                                             cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct            <  1:1617789/93‑1 (MQ=255)
                                                                             cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct            <  1:6586877/93‑1 (MQ=255)
                                                                             cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct            <  1:4932268/93‑1 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc           <  1:5358743/93‑1 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc           <  1:2639928/93‑1 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc           <  2:4939218/93‑1 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc           <  2:4104353/93‑1 (MQ=255)
                                                                               tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct          <  2:3491495/93‑1 (MQ=255)
                                                                                cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt         <  1:6390834/93‑1 (MQ=255)
                                                                                cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt         <  2:3343594/93‑1 (MQ=255)
                                                                                 ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt        >  2:3511790/1‑93 (MQ=255)
                                                                                  cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta       <  2:4109500/93‑1 (MQ=255)
                                                                                  cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta       >  1:5456824/1‑93 (MQ=255)
                                                                                   gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat      <  2:5647478/93‑1 (MQ=255)
                                                                                   gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat      >  1:1597432/1‑93 (MQ=255)
                                                                                    aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata     >  2:4465750/1‑93 (MQ=255)
                                                                                     cGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAg    <  2:2151518/93‑1 (MQ=255)
                                                                                       cAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcg  <  1:6129484/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG  >  NC_000913/4270122‑4270301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  >  NC_000913/4270119‑4270303
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GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGG                                                                                             <  SRR4302536.5457374/94‑1 (MQ=60)
 TTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGC                                                                                            <  SRR4302536.4142616/94‑1 (MQ=60)
  TGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCG                                                                                           <  SRR4302536.1657271/94‑1 (MQ=60)
   GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGC                                                                                          >  SRR4302536.2831556/1‑94 (MQ=60)
     AGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGT                                                                                        >  SRR4302536.2689106/1‑94 (MQ=60)
         TTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCT                                                                                    <  SRR4302536.5221301/94‑1 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   >  SRR4302536.5752880/1‑94 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   <  SRR4302536.5589037/94‑1 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   <  SRR4302536.3411612/94‑1 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   <  SRR4302536.532511/94‑1 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   <  SRR4302536.4565518/94‑1 (MQ=60)
            CATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCG                                                                                 >  SRR4302536.2409028/1‑94 (MQ=60)
            CATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCG                                                                                 >  SRR4302536.5926797/1‑94 (MQ=60)
             ATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGC                                                                                >  SRR4302536.5233155/1‑94 (MQ=60)
                GCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGC                                                                             <  SRR4302536.4956870/94‑1 (MQ=60)
                 CATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCT                                                                            >  SRR4302536.2615672/1‑94 (MQ=60)
                 CATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCT                                                                            >  SRR4302536.9255/1‑94 (MQ=60)
                 CATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCT                                                                            <  SRR4302536.2757209/94‑1 (MQ=60)
                 CATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCT                                                                            >  SRR4302536.6839889/1‑94 (MQ=60)
                 CATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCT                                                                            >  SRR4302536.2779859/1‑94 (MQ=60)
                 CATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCT                                                                            >  SRR4302536.489078/1‑94 (MQ=60)
                  ATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTG                                                                           >  SRR4302536.1451412/1‑94 (MQ=60)
                    GACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGG                                                                         >  SRR4302536.3260940/1‑94 (MQ=60)
                     ACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCG                                                                        >  SRR4302536.5699212/1‑94 (MQ=60)
                     ACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACACACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCG                                                                        >  SRR4302536.6511916/1‑94 (MQ=60)
                      CCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGC                                                                       <  SRR4302536.1468484/94‑1 (MQ=60)
                      CCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGC                                                                       >  SRR4302536.5936706/1‑94 (MQ=60)
                      CCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGC                                                                       <  SRR4302536.3350714/94‑1 (MQ=60)
                       CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                      >  SRR4302536.2990594/1‑94 (MQ=60)
                         ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                    <  SRR4302536.6319018/94‑1 (MQ=60)
                          TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                   <  SRR4302536.5759695/94‑1 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCANNCNNNTCCCTTCGCAGCnnnnncgcgnac                                                                 >  SRR4302536.6075735/1‑82 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 >  SRR4302536.898381/1‑94 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 >  SRR4302536.6080434/1‑94 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 <  SRR4302536.5318390/94‑1 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 >  SRR4302536.1780780/1‑94 (MQ=60)
                             TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                <  SRR4302536.6684090/94‑1 (MQ=60)
                             TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                <  SRR4302536.5611176/94‑1 (MQ=60)
                               ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                              >  SRR4302536.6658826/1‑94 (MQ=60)
                               ACGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCCCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                              <  SRR4302536.4803392/94‑1 (MQ=60)
                                TGTGGGAGAGAGGAGGGAGCGGCGTATTCTCGACATACGCGGGGCGCGCTTCCCGCAGCCGGCGCGTCCCTTCGCAGCTGGGGCGCGGACGATT                                                             <  SRR4302536.3114342/94‑1 (MQ=28)
                                TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACCCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                             <  SRR4302536.6349305/94‑1 (MQ=60)
                                TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                             <  SRR4302536.4749200/94‑1 (MQ=60)
                                TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                             <  SRR4302536.1763910/94‑1 (MQ=60)
                                 GTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTA                                                            >  SRR4302536.3866424/1‑94 (MQ=60)
                                  TCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAC                                                           <  SRR4302536.3910889/94‑1 (MQ=60)
                                   CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                          >  SRR4302536.5964001/1‑94 (MQ=60)
                                   CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                          >  SRR4302536.1124468/1‑94 (MQ=60)
                                   CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                          <  SRR4302536.1992587/94‑1 (MQ=60)
                                   CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAAT                                                          <  SRR4302536.3584839/94‑1 (MQ=60)
                                    GGTGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                         <  SRR4302536.1856251/94‑1 (MQ=60)
                                    GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                         <  SRR4302536.2549165/94‑1 (MQ=60)
                                    GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                         <  SRR4302536.433391/94‑1 (MQ=60)
                                     GAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGA                                                        <  SRR4302536.1330816/94‑1 (MQ=60)
                                      AGATAGTAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       <  SRR4302536.6642407/94‑1 (MQ=60)
                                      AGATAGCGGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       <  SRR4302536.261000/94‑1 (MQ=60)
                                      AGATAGCAGGCAGCTGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       <  SRR4302536.2248018/94‑1 (MQ=60)
                                      AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       <  SRR4302536.2897033/94‑1 (MQ=60)
                                      AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       <  SRR4302536.5512225/94‑1 (MQ=60)
                                      AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       >  SRR4302536.3483346/1‑94 (MQ=60)
                                      AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       >  SRR4302536.5750471/1‑94 (MQ=60)
                                       GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                      <  SRR4302536.4089213/94‑1 (MQ=60)
                                         TAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAA                                                    >  SRR4302536.5727289/1‑94 (MQ=60)
                                          AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                   <  SRR4302536.1662419/94‑1 (MQ=60)
                                          AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                   >  SRR4302536.3686130/1‑94 (MQ=60)
                                           GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                  >  SRR4302536.19806/1‑94 (MQ=60)
                                           GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                  <  SRR4302536.6081386/94‑1 (MQ=60)
                                           GCAGGCAGCAGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                  >  SRR4302536.6760272/1‑94 (MQ=60)
                                              GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                               <  SRR4302536.4274979/94‑1 (MQ=60)
                                              GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                               <  SRR4302536.5425410/94‑1 (MQ=60)
                                                 AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                            >  SRR4302536.2944930/1‑94 (MQ=60)
                                                 AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                            <  SRR4302536.2219448/94‑1 (MQ=60)
                                                 AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                            <  SRR4302536.4578746/94‑1 (MQ=60)
                                                   CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                          <  SRR4302536.5975591/94‑1 (MQ=60)
                                                   CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                          >  SRR4302536.4035559/1‑94 (MQ=60)
                                                    GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                         <  SRR4302536.5030003/94‑1 (MQ=60)
                                                    GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                         >  SRR4302536.162845/1‑94 (MQ=60)
                                                      tggATTCTCGCCATACACGTGGCGCGCTTCCCGACGAGGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                       <  SRR4302536.748772/91‑1 (MQ=60)
                                                      CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                       >  SRR4302536.5420584/1‑94 (MQ=60)
                                                      CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                       >  SRR4302536.1072685/1‑94 (MQ=60)
                                                      CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                       <  SRR4302536.4081249/94‑1 (MQ=60)
                                                      CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                       >  SRR4302536.1700344/1‑94 (MQ=60)
                                                       GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                      <  SRR4302536.872904/94‑1 (MQ=60)
                                                        TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                     >  SRR4302536.778461/1‑94 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    >  SRR4302536.4727765/1‑94 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    >  SRR4302536.6465022/1‑94 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    >  SRR4302536.96403/1‑94 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302536.1700402/94‑1 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302536.585426/94‑1 (MQ=60)
                                                          TTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGC                                   <  SRR4302536.3302767/94‑1 (MQ=60)
                                                            CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                 >  SRR4302536.3657149/1‑94 (MQ=60)
                                                              CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTGGCAGCTGGAGCGCGGACGTTTACTGATAAACTTGGCTAATcgctcgcggac                               >  SRR4302536.940795/1‑83 (MQ=60)
                                                              CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                               >  SRR4302536.2120090/1‑94 (MQ=60)
                                                              CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                               >  SRR4302536.4737035/1‑94 (MQ=60)
                                                                GCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCG                             <  SRR4302536.2154804/94‑1 (MQ=60)
                                                                 CATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGC                            >  SRR4302536.2155731/1‑94 (MQ=60)
                                                                 CATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGC                            >  SRR4302536.2785630/1‑94 (MQ=60)
                                                                 CATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGC                            >  SRR4302536.3087699/1‑94 (MQ=60)
                                                                   taacCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGC                          <  SRR4302536.587839/90‑1 (MQ=60)
                                                                    ACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCT                         >  SRR4302536.1961153/1‑94 (MQ=60)
                                                                     CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTG                        <  SRR4302536.1971285/94‑1 (MQ=60)
                                                                     CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTG                        <  SRR4302536.1637475/94‑1 (MQ=60)
                                                                      ACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGG                       <  SRR4302536.2081966/94‑1 (MQ=60)
                                                                      ACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGG                       >  SRR4302536.1637630/1‑94 (MQ=60)
                                                                        GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTTTTCGCGTACCGCGCTGGAT                     >  SRR4302536.3554284/1‑94 (MQ=60)
                                                                        GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                     <  SRR4302536.1850708/94‑1 (MQ=60)
                                                                         TGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATT                    <  SRR4302536.5373211/94‑1 (MQ=60)
                                                                         TGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATT                    <  SRR4302536.5087176/94‑1 (MQ=60)
                                                                            CGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCG                 >  SRR4302536.1088668/1‑94 (MQ=60)
                                                                             GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                >  SRR4302536.2979582/1‑94 (MQ=60)
                                                                              CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC               <  SRR4302536.5415238/94‑1 (MQ=60)
                                                                                CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC             >  SRR4302536.5648951/1‑94 (MQ=60)
                                                                                CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC             <  SRR4302536.1664927/94‑1 (MQ=60)
                                                                                CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC             <  SRR4302536.6782839/94‑1 (MQ=60)
                                                                                CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC             <  SRR4302536.5080464/94‑1 (MQ=60)
                                                                                 TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT            >  SRR4302536.966407/1‑94 (MQ=60)
                                                                                 TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT            <  SRR4302536.2719062/94‑1 (MQ=60)
                                                                                 TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT            <  SRR4302536.5518633/94‑1 (MQ=60)
                                                                                  TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT           <  SRR4302536.1260098/94‑1 (MQ=60)
                                                                                  TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT           <  SRR4302536.1691022/94‑1 (MQ=60)
                                                                                  TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT           <  SRR4302536.1370954/94‑1 (MQ=60)
                                                                                   CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT          <  SRR4302536.4120589/94‑1 (MQ=60)
                                                                                   CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT          <  SRR4302536.6581517/94‑1 (MQ=60)
                                                                                    CCGAGGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAACCTTGGCTAATTCTTCGCGTACCGCGCTGGATT‑CGTCTCTTTAT        <  SRR4302536.3149665/94‑1 (MQ=60)
                                                                                    CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA         >  SRR4302536.5619991/1‑94 (MQ=60)
                                                                                    CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA         <  SRR4302536.2708178/94‑1 (MQ=60)
                                                                                     CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT        >  SRR4302536.1644092/1‑94 (MQ=60)
                                                                                      GACGCAGGCGCGTCCCTTCGCGGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATA       >  SRR4302536.6264308/1‑94 (MQ=60)
                                                                                       ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTCCCGCGCTGGATTCCGTCTCTTTATAG      <  SRR4302536.1596468/94‑1 (MQ=60)
                                                                                       ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG      <  SRR4302536.1249097/94‑1 (MQ=60)
                                                                                        CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC     >  SRR4302536.4057890/1‑94 (MQ=60)
                                                                                          CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG   <  SRR4302536.6312164/94‑1 (MQ=60)
                                                                                           AGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTACTTCTTCGCGTACCGCGCTTGATTCCGTCTCTTTATAGCGGC  >  SRR4302536.2242190/1‑94 (MQ=60)
                                                                                           AGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  <  SRR4302536.435884/94‑1 (MQ=60)
                                                                                             |                                                                                           
GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  >  NC_000913/4270119‑4270303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑