Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,480	A→G	F256S (TTC→TCC)	tyrS ←	tyrosine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,480	0	A	G	99.6%	740.5 / ‑4.6	226	F256S (TTC→TCC)	tyrS	tyrosine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (116/109); minor base T (1/0); total (117/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 231 total aligned reads displayed.

AGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAG  >  NC_000913/1714390‑1714568

aGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAt                                                                                        >  1:1751501/1‑93 (MQ=255)

 gggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAtt                                                                                       >  1:4793542/1‑93 (MQ=255)

   gCGTTGATCTCTTCAATACTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTg                                                                                     <  2:3406358/93‑1 (MQ=255)

    cattGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGt                                                                                    <  1:3861140/91‑1 (MQ=255)

     gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTa                                                                                   <  2:1275891/93‑1 (MQ=255)

      ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGTAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAc                                                                                  >  2:4821311/1‑93 (MQ=255)

      ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAc                                                                                  >  1:4869398/1‑93 (MQ=255)

      ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAc                                                                                  <  2:3605914/93‑1 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                <  2:6139992/93‑1 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                >  2:222022/1‑93 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                >  1:2196665/1‑93 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                >  2:5587014/1‑93 (MQ=255)

         aTCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACggt                                                                               >  2:282064/1‑92 (MQ=255)

          tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGc                                                                              >  2:3160035/1‑93 (MQ=255)

          tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGc                                                                              <  2:5111709/93‑1 (MQ=255)

          tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGc                                                                              <  1:4351767/93‑1 (MQ=255)

           ctctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCt                                                                             >  1:6642764/1‑93 (MQ=255)

            tctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTg                                                                            >  2:3725469/1‑93 (MQ=255)

             ctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTgg                                                                           <  1:5503895/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                          <  2:439185/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                          <  2:719497/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                          >  1:2384202/1‑93 (MQ=255)

               tCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtt                                                                         >  2:5701355/1‑93 (MQ=255)

               tCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtt                                                                         >  1:6564465/1‑93 (MQ=255)

                 aaTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                       <  2:1619736/93‑1 (MQ=255)

                 aaTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                       <  2:3869052/93‑1 (MQ=255)

                 aaTGCTCATAAAGAAGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                       <  1:5122486/93‑1 (MQ=255)

                       cATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCgg                                                                 >  2:5038134/1‑93 (MQ=255)

                        aTAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGa                                                                <  2:3789022/93‑1 (MQ=255)

                         tAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGAt                                                               >  1:240479/1‑93 (MQ=255)

                         tAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGAt                                                               >  1:3947234/1‑93 (MQ=255)

                          aaaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATc                                                              <  1:5652846/93‑1 (MQ=255)

                           aaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATcc                                                             >  1:2982899/1‑93 (MQ=255)

                            aGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCa                                                            <  1:5391704/93‑1 (MQ=255)

                             ggTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCaa                                                           >  2:2031582/1‑93 (MQ=255)

                               tGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAAcc                                                         >  1:4475645/1‑93 (MQ=255)

                               tGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAAcc                                                         >  2:6424237/1‑93 (MQ=255)

                                gaagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCa                                                        <  1:5099540/93‑1 (MQ=255)

                                 aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGCACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAg                                                       >  1:1025373/1‑93 (MQ=255)

                                 aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAg                                                       >  1:6445945/1‑93 (MQ=255)

                                   gaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAc                                                     <  2:1687310/93‑1 (MQ=255)

                                   gaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAc                                                     >  2:4433773/1‑93 (MQ=255)

                                   gaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAc                                                     <  2:662419/93‑1 (MQ=255)

                                    aaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                                    >  1:6299811/1‑93 (MQ=255)

                                    aaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                                    <  2:2192142/93‑1 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   <  1:803899/93‑1 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   >  1:5935666/1‑93 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   >  1:4646902/1‑93 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   >  2:4170435/1‑93 (MQ=255)

                                      cTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgc                                                  <  2:2988105/93‑1 (MQ=255)

                                        tCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcgc                                                <  2:5667631/93‑1 (MQ=255)

                                         cAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgcc                                               >  1:3235539/1‑93 (MQ=255)

                                          aGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccg                                              <  2:1010767/93‑1 (MQ=255)

                                          aGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccg                                              >  1:6712468/1‑93 (MQ=255)

                                            gAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCgccgcc                                            >  1:6625912/1‑93 (MQ=255)

                                            gAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCgccgcc                                            <  1:2600628/93‑1 (MQ=255)

                                             aaGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCt                                           <  2:4252322/93‑1 (MQ=255)

                                             aaGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCt                                           >  1:6039472/1‑93 (MQ=255)

                                              aGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCtt                                          >  1:4560409/1‑93 (MQ=255)

                                              aGCGGTAAACGTCGGCATCCGCAGTGTTGATCAAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCtt                                          <  1:1081230/93‑1 (MQ=255)

                                               gCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTc                                         <  1:424224/93‑1 (MQ=255)

                                                  gTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGt                                      <  1:6087653/93‑1 (MQ=255)

                                                    aagcGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGttt                                    <  1:1392568/90‑1 (MQ=255)

                                                    aaaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGttt                                    >  2:5788348/1‑93 (MQ=255)

                                                     aaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtttt                                   >  2:1766054/1‑93 (MQ=255)

                                                     aaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtttt                                   <  2:6005126/93‑1 (MQ=255)

                                                      aCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTa                                  >  1:432587/1‑93 (MQ=255)

                                                      aCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTa                                  >  2:2400119/1‑93 (MQ=255)

                                                        gTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAcc                                >  2:2145674/1‑93 (MQ=255)

                                                         tCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCa                               >  2:4521067/1‑93 (MQ=255)

                                                            gCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTATCAAAt                            >  1:932053/1‑93 (MQ=255)

                                                            gCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAt                            >  1:1853157/1‑93 (MQ=255)

                                                               tCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTg                         >  1:2042057/1‑93 (MQ=255)

                                                               tCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTg                         <  1:5601319/93‑1 (MQ=255)

                                                                 cGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTggg                       >  1:4998243/1‑92 (MQ=255)

                                                                 cGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGt                       <  2:3983327/93‑1 (MQ=255)

                                                                   cAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGc                     <  2:5914718/93‑1 (MQ=255)

                                                                     gtgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                   >  2:4901910/1‑93 (MQ=255)

                                                                     gtgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                   >  1:2000767/1‑93 (MQ=255)

                                                                       gtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                 >  1:1229662/1‑93 (MQ=255)

                                                                       gtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                 >  1:1714396/1‑93 (MQ=255)

                                                                        ttGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCt                >  1:1315682/1‑93 (MQ=255)

                                                                        ttGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCt                >  1:3170491/1‑93 (MQ=255)

                                                                         tGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg               <  2:3963019/93‑1 (MQ=255)

                                                                         tGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg               <  1:148244/93‑1 (MQ=255)

                                                                         tGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg               >  2:2507349/1‑93 (MQ=255)

                                                                           aTCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCt             >  1:3621158/1‑93 (MQ=255)

                                                                           aTCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCt             >  1:2745495/1‑93 (MQ=255)

                                                                            tCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt            <  2:3038025/93‑1 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         <  2:4450389/93‑1 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         <  1:2486137/93‑1 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         >  1:2805321/1‑93 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         <  1:4047089/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTCCGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa   <  2:1496238/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGGGCCGCCTTCAGTTATACCAAATTTGGTGCCATCTGCTTTAGTGATCa   >  1:6181619/1‑93 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa   <  2:460791/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa   <  1:1521390/93‑1 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAg  <  2:4119525/93‑1 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAg  <  2:1812480/93‑1 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAg  >  1:4218134/1‑93 (MQ=255)

AGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAG  >  NC_000913/1714390‑1714568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAA  >  NC_000913/1714388‑1714573

CCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGA                                                                                              >  SRR4302536.606020/1‑94 (MQ=60)

CCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTCGTAGGA                                                                                              >  SRR4302536.4725678/1‑94 (MQ=60)

 CAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAT                                                                                             >  SRR4302536.1803063/1‑94 (MQ=60)

 CAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAT                                                                                             <  SRR4302536.2025902/94‑1 (MQ=60)

 CAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAT                                                                                             >  SRR4302536.3320059/1‑94 (MQ=60)

 CAGGGCGTCGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGCAGGCATCCGCAGTGTTGATCCAGAACTGGGAGGAT                                                                                             >  SRR4302536.5131124/1‑94 (MQ=60)

  AGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATT                                                                                            >  SRR4302536.4937829/1‑94 (MQ=60)

      CGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTA                                                                                        <  SRR4302536.6429085/94‑1 (MQ=60)

      CATTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTA                                                                                        <  SRR4302536.3976033/94‑1 (MQ=60)

       GTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGCACTGGTAGGATTTGTAG                                                                                       >  SRR4302536.4278236/1‑94 (MQ=60)

       GTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAC                                                                                       >  SRR4302536.5015913/1‑94 (MQ=60)

       GTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAC                                                                                       >  SRR4302536.1729545/1‑94 (MQ=60)

        TTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACG                                                                                      <  SRR4302536.2107137/94‑1 (MQ=60)

         TGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGG                                                                                     >  SRR4302536.2262974/1‑94 (MQ=60)

          GATCTCTTCGATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGG                                                                                    >  SRR4302536.6763277/1‑94 (MQ=60)

            TCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCT                                                                                  >  SRR4302536.6840280/1‑94 (MQ=60)

            TCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCT                                                                                  <  SRR4302536.4481963/94‑1 (MQ=60)

             CTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTG                                                                                 <  SRR4302536.4628430/94‑1 (MQ=60)

              TCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGG                                                                                <  SRR4302536.1838060/94‑1 (MQ=60)

              TCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGG                                                                                <  SRR4302536.2298072/94‑1 (MQ=60)

               CTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGT                                                                               >  SRR4302536.2456414/1‑94 (MQ=60)

               CTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGT                                                                               <  SRR4302536.5668105/94‑1 (MQ=60)

                TTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTT                                                                              >  SRR4302536.6759861/1‑94 (MQ=60)

                TTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTT                                                                              <  SRR4302536.5648758/94‑1 (MQ=60)

                 TCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTCGGATTTGTACTGGCTGGTTT                                                                             >  SRR4302536.2464970/1‑94 (MQ=60)

                 TCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTT                                                                             <  SRR4302536.4573458/94‑1 (MQ=60)

                  CAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTT                                                                            <  SRR4302536.4997296/94‑1 (MQ=60)

                   AATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTC                                                                           >  SRR4302536.3167211/1‑94 (MQ=60)

                   AATGCTCATAAAGAAGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTC                                                                           <  SRR4302536.5275537/94‑1 (MQ=60)

                    ATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCT                                                                          <  SRR4302536.5918787/94‑1 (MQ=60)

                     TGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTT                                                                         <  SRR4302536.6504256/94‑1 (MQ=60)

                     TGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTT                                                                         <  SRR4302536.6909604/94‑1 (MQ=60)

                      GCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTC                                                                        <  SRR4302536.250772/94‑1 (MQ=60)

                        TCATAAAGGTGAAGAGCTTCAGCAAGCGGTACACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGG                                                                      <  SRR4302536.991566/94‑1 (MQ=60)

                        TCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGG                                                                      <  SRR4302536.6399642/94‑1 (MQ=60)

                          ATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGAT                                                                    >  SRR4302536.246878/1‑94 (MQ=60)

                          ATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGAT                                                                    >  SRR4302536.4064478/1‑94 (MQ=60)

                          ATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGGTTTCTTCGGAT                                                                    >  SRR4302536.4800141/1‑94 (MQ=60)

                            AAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCC                                                                  <  SRR4302536.1962659/94‑1 (MQ=60)

                            AAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCC                                                                  >  SRR4302536.3070696/1‑94 (MQ=60)

                            AAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCC                                                                  <  SRR4302536.5820837/94‑1 (MQ=60)

                             AAGGTGAGGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGGTCCA                                                                 >  SRR4302536.2742816/1‑94 (MQ=60)

                             AAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATGCA                                                                 >  SRR4302536.2660242/1‑94 (MQ=60)

                              AGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAA                                                                <  SRR4302536.5552667/94‑1 (MQ=60)

                              AGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAA                                                                <  SRR4302536.6824970/94‑1 (MQ=60)

                                GTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACC                                                              >  SRR4302536.4610098/1‑94 (MQ=60)

                                 TGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCA                                                             >  SRR4302536.4460079/1‑94 (MQ=60)

                                 TGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCA                                                             <  SRR4302536.1420908/94‑1 (MQ=60)

                                  GAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGCACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAG                                                            >  SRR4302536.1055736/1‑94 (MQ=60)

                                  GAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAG                                                            >  SRR4302536.6638530/1‑94 (MQ=60)

                                  GAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAG                                                            <  SRR4302536.5252023/94‑1 (MQ=60)

                                   AAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGTATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGA                                                           >  SRR4302536.551276/1‑94 (MQ=60)

                                   AAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGA                                                           >  SRR4302536.3877849/1‑94 (MQ=60)

                                    AGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAC                                                          >  SRR4302536.6353210/1‑94 (MQ=60)

                                    AGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAC                                                          >  SRR4302536.880059/1‑94 (MQ=60)

                                     GAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACT                                                         >  SRR4302536.6772609/1‑94 (MQ=60)

                                     GAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACT                                                         >  SRR4302536.6487482/1‑94 (MQ=60)

                                      AACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTG                                                        <  SRR4302536.13870/94‑1 (MQ=60)

                                      AACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTG                                                        >  SRR4302536.4786611/1‑94 (MQ=60)

                                      AACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTG                                                        >  SRR4302536.6112470/1‑94 (MQ=60)

                                       ACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGC                                                       <  SRR4302536.827471/94‑1 (MQ=60)

                                        CTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCG                                                      <  SRR4302536.777060/94‑1 (MQ=60)

                                          TCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCC                                                    >  SRR4302536.3330384/1‑94 (MQ=60)

                                           CAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCG                                                   >  SRR4302536.6911746/1‑94 (MQ=60)

                                            AGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGC                                                  >  SRR4302536.4051727/1‑94 (MQ=60)

                                            AGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGC                                                  >  SRR4302536.6018209/1‑94 (MQ=60)

                                             GGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCC                                                 >  SRR4302536.6822989/1‑94 (MQ=60)

                                              GAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCT                                                <  SRR4302536.3904487/94‑1 (MQ=60)

                                              GAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCT                                                <  SRR4302536.2678712/94‑1 (MQ=60)

                                              GAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCT                                                >  SRR4302536.6219591/1‑94 (MQ=60)

                                               AAGCGGTAACTGTCGACATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTT                                               <  SRR4302536.3381952/94‑1 (MQ=60)

                                               AAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTT                                               >  SRR4302536.4697226/1‑94 (MQ=60)

                                               AAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTT                                               <  SRR4302536.6258411/94‑1 (MQ=60)

                                                AGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTC                                              >  SRR4302536.1070240/1‑94 (MQ=60)

                                                AGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTC                                              >  SRR4302536.523018/1‑94 (MQ=60)

                                                AGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTC                                              <  SRR4302536.4427319/94‑1 (MQ=60)

                                                AGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTGC                                              <  SRR4302536.2867909/94‑1 (MQ=60)

                                                AGCGGTAAACGTCGGCATCCGCAGTGTTGATCAAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTC                                              <  SRR4302536.1113603/94‑1 (MQ=60)

                                                 GCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCA                                             <  SRR4302536.435399/94‑1 (MQ=60)

                                                 GCGGTAAACGTCGGCATCCGCAGTGTTGATACAGAACTGGAAGGATTTTTACCGGCTGGTTTTTTTAGGCTCCAACCAGACTTCGCCGCCTgct                                             >  SRR4302536.33349/1‑91 (MQ=47)

                                                  CGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCCACCCGACTGCGGCGCCTTCAG                                            >  SRR4302536.202605/1‑94 (MQ=60)

                                                  CGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAG                                            <  SRR4302536.4308486/94‑1 (MQ=60)

                                                   GGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGT                                           >  SRR4302536.69799/1‑94 (MQ=60)

                                                   GGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGT                                           <  SRR4302536.1802635/94‑1 (MQ=60)

                                                    GTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCCGTT                                          >  SRR4302536.5017382/1‑94 (MQ=60)

                                                    GTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTT                                          <  SRR4302536.6269225/94‑1 (MQ=60)

                                                    GTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTT                                          >  SRR4302536.2313838/1‑94 (MQ=60)

                                                      AAGCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTT                                        <  SRR4302536.1434034/94‑1 (MQ=60)

                                                      AAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTT                                        >  SRR4302536.4774482/1‑94 (MQ=60)

                                                      AAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTT                                        >  SRR4302536.1338140/1‑94 (MQ=60)

                                                       AACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTA                                       >  SRR4302536.443964/1‑94 (MQ=60)

                                                       AACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTCTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTA                                       >  SRR4302536.1442893/1‑94 (MQ=60)

                                                          GTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCA                                    <  SRR4302536.2062342/94‑1 (MQ=60)

                                                          GTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCA                                    >  SRR4302536.6169390/1‑94 (MQ=60)

                                                           TCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAA                                   <  SRR4302536.4360558/94‑1 (MQ=60)

                                                             GGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTATCAAAT                                 >  SRR4302536.959505/1‑94 (MQ=60)

                                                             GGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAT                                 >  SRR4302536.1909006/1‑94 (MQ=60)

                                                               CATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTT                               <  SRR4302536.6673335/94‑1 (MQ=60)

                                                                ATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTG                              >  SRR4302536.3133924/1‑94 (MQ=60)

                                                                ATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTG                              >  SRR4302536.4814341/1‑94 (MQ=60)

                                                                ATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTG                              >  SRR4302536.5758983/1‑94 (MQ=60)

                                                                ATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTG                              >  SRR4302536.2103547/1‑94 (MQ=60)

                                                                 TCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGT                             >  SRR4302536.5814998/1‑94 (MQ=60)

                                                                 TCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGG                             <  SRR4302536.5767796/94‑1 (MQ=60)

                                                                  CCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGG                            >  SRR4302536.5148241/1‑94 (MQ=60)

                                                                   CGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCATATTTACCAAATTTTGTG                           >  SRR4302536.1666827/1‑94 (MQ=60)

                                                                   CGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTG                           <  SRR4302536.5600236/94‑1 (MQ=60)

                                                                      AGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCA                        >  SRR4302536.4098732/1‑94 (MQ=60)

                                                                      AGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCA                        >  SRR4302536.2060979/1‑94 (MQ=60)

                                                                        TGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATC                      >  SRR4302536.1763967/1‑94 (MQ=60)

                                                                        TGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATC                      >  SRR4302536.1266588/1‑94 (MQ=60)

                                                                         GTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCT                     >  SRR4302536.3263386/1‑94 (MQ=60)

                                                                         GTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCT                     >  SRR4302536.1354961/1‑94 (MQ=60)

                                                                          TTGATCCAGAACTGGGAGGAGTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGGTTTACCCAATTTGGTGCCATCTG                    >  SRR4302536.524257/1‑94 (MQ=60)

                                                                           TGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGC                   <  SRR4302536.152102/94‑1 (MQ=60)

                                                                           TGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGC                   <  SRR4302536.3839500/94‑1 (MQ=60)

                                                                           TGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGC                   <  SRR4302536.1221292/94‑1 (MQ=60)

                                                                            GATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCT                  >  SRR4302536.3729425/1‑94 (MQ=60)

                                                                            GATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCT                  >  SRR4302536.2827258/1‑94 (MQ=60)

                                                                              TCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTT                <  SRR4302536.5178424/94‑1 (MQ=60)

                                                                              TCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTT                <  SRR4302536.2744629/94‑1 (MQ=60)

                                                                                CAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAG              >  SRR4302536.2888515/1‑94 (MQ=60)

                                                                                 AGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGT             <  SRR4302536.4167054/94‑1 (MQ=60)

                                                                                 AGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGT             <  SRR4302536.2561150/94‑1 (MQ=60)

                                                                                 AGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGT             >  SRR4302536.4565436/1‑94 (MQ=60)

                                                                                 AGAACTGGAAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCCGACTGCGCCGCCTTCAGATTTACCAAATTTGGTGCCATCTCCTTTAGA             >  SRR4302536.2937575/1‑94 (MQ=60)

                                                                                     CTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATC         >  SRR4302536.1534736/1‑94 (MQ=60)

                                                                                      TGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGGGCCGCCTTCAGTTATACCAAATTTGGTGCCATCTGCTTTAGTGATCA        >  SRR4302536.6365727/1‑94 (MQ=60)

                                                                                       GGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAG       <  SRR4302536.1566167/94‑1 (MQ=60)

                                                                                       GGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAG       >  SRR4302536.4343606/1‑94 (MQ=60)

                                                                                         TAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCG     >  SRR4302536.5170830/1‑94 (MQ=60)

                                                                                            GATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAA  <  SRR4302536.2596341/94‑1 (MQ=60)

                                                                                            GATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAA  <  SRR4302536.780592/94‑1 (MQ=60)

CCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAA  >  NC_000913/1714388‑1714573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`