Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,874 T→C H225R (CAT→CGT)  b1680 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,8740TC99.6% 792.7 / ‑4.8 243H225R (CAT→CGT) b1680hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (143/99);  total (143/100)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

Only 100 of 247 total aligned reads displayed.

ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT  >  NC_000913/1757784‑1757962
                                                                                          |                                                                                        
 tAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGcc                                                                                       >  2:2846107/1‑93 (MQ=255)
 tAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGcc                                                                                       >  1:4529952/1‑93 (MQ=255)
   gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCg                                                                                     >  1:839781/1‑93 (MQ=255)
     aCCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGga                                                                                   <  2:3111208/93‑1 (MQ=255)
     aCCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTCCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGga                                                                                   >  2:1017781/1‑93 (MQ=255)
         cccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAac                                                                               <  1:5507350/93‑1 (MQ=255)
         cccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAac                                                                               >  1:1844057/1‑93 (MQ=255)
         cccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAac                                                                               <  1:2855748/93‑1 (MQ=255)
          ccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAaca                                                                              >  1:2548393/1‑93 (MQ=255)
           cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                             >  2:6448967/1‑93 (MQ=255)
           cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                             >  1:4841092/1‑93 (MQ=255)
           cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                             <  1:474811/93‑1 (MQ=255)
            ccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACg                                                                            >  2:861128/1‑93 (MQ=255)
              ttCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTa                                                                          >  1:2644329/1‑93 (MQ=255)
              ttCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTa                                                                          >  1:4597351/1‑93 (MQ=255)
               tCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaa                                                                         <  2:4538718/93‑1 (MQ=255)
                cccACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaaa                                                                        <  2:1444777/93‑1 (MQ=255)
                 ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaaaa                                                                       <  1:2593711/93‑1 (MQ=255)
                 ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaaaa                                                                       <  2:4442234/93‑1 (MQ=255)
                 ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaaaa                                                                       <  2:756026/93‑1 (MQ=255)
                   aCGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGt                                                                     <  1:3697279/93‑1 (MQ=255)
                    cggcggCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTc                                                                    >  2:1279305/1‑93 (MQ=255)
                      gcggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGc                                                                  >  1:2786056/1‑93 (MQ=255)
                      gcggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGc                                                                  >  2:1145912/1‑93 (MQ=255)
                        ggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCaa                                                                <  1:1390556/93‑1 (MQ=255)
                         gcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAt                                                               >  2:5690683/1‑93 (MQ=255)
                         gcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAt                                                               >  2:6724552/1‑93 (MQ=255)
                          cATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATc                                                              >  2:1890872/1‑93 (MQ=255)
                           aTCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATcc                                                             <  2:6200364/93‑1 (MQ=255)
                            tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            >  2:4589906/1‑93 (MQ=255)
                            tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            >  2:5186074/1‑93 (MQ=255)
                            tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            >  2:4018516/1‑93 (MQ=255)
                            tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            <  2:2390465/93‑1 (MQ=255)
                            tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATAAAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            <  1:3164152/93‑1 (MQ=255)
                             ctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAg                                                           >  1:4300461/1‑93 (MQ=255)
                                cTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAgcgc                                                        >  2:3548722/1‑93 (MQ=255)
                                cTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAgcgc                                                        >  2:4140367/1‑93 (MQ=255)
                                 tGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGcc                                                       >  2:1331582/1‑93 (MQ=255)
                                    aacaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGa                                                    <  2:822811/93‑1 (MQ=255)
                                     acaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGaa                                                   >  2:2028578/1‑93 (MQ=255)
                                     acaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGaa                                                   >  2:4668667/1‑93 (MQ=255)
                                     acaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGaa                                                   <  1:6306677/93‑1 (MQ=255)
                                      caaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAc                                                  >  1:2178314/1‑93 (MQ=255)
                                      caaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAc                                                  <  1:5012428/93‑1 (MQ=255)
                                      aaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGTCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAc                                                  <  2:2534576/92‑1 (MQ=255)
                                       aaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACa                                                 <  1:4001790/93‑1 (MQ=255)
                                       aaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACa                                                 >  1:1915571/1‑93 (MQ=255)
                                       aaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACa                                                 >  1:128964/1‑93 (MQ=255)
                                        aGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAt                                                >  2:6360422/1‑93 (MQ=255)
                                         ggCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATc                                               >  1:1524111/1‑93 (MQ=255)
                                          gCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATcc                                              >  1:1669316/1‑93 (MQ=255)
                                          gCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATcc                                              <  1:6475138/93‑1 (MQ=255)
                                           cttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCa                                             >  2:3371093/1‑93 (MQ=255)
                                            ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                            >  2:3284742/1‑93 (MQ=255)
                                             tcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAcc                                           >  2:1365092/1‑93 (MQ=255)
                                               tttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCgg                                         >  2:3834804/1‑93 (MQ=255)
                                               tttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCgg                                         >  2:4774949/1‑93 (MQ=255)
                                               tttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCgg                                         >  1:397037/1‑93 (MQ=255)
                                               tttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCAACCgg                                         >  2:145578/1‑93 (MQ=255)
                                                  cacaTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgatg                                      >  2:4312302/1‑93 (MQ=255)
                                                    caTAAAGAATGCCAATTCCGGTGGTGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgatgat                                    <  1:2661937/93‑1 (MQ=255)
                                                    caTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgatgat                                    >  1:3408734/1‑93 (MQ=255)
                                                     aTAAAGAATGCCAATTCCGGTGTGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGGAATCCAGCGCCTGATCATACATCTgctgatt                                   >  1:5868056/1‑92 (MQ=255)
                                                     aTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGatgatg                                   >  1:22647/1‑93 (MQ=255)
                                                     aTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGatgatg                                   <  2:5321309/93‑1 (MQ=255)
                                                     aTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGatgatg                                   >  1:318677/1‑93 (MQ=255)
                                                      tAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGc                                  >  1:150482/1‑93 (MQ=255)
                                                       aaaGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCa                                 <  1:6211129/93‑1 (MQ=255)
                                                        aaGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAt                                >  1:3801426/1‑93 (MQ=255)
                                                         aGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATc                               >  2:2949620/1‑93 (MQ=255)
                                                         aGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATc                               >  2:2056303/1‑93 (MQ=255)
                                                         aGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATc                               >  1:6593180/1‑93 (MQ=255)
                                                          gAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCa                              <  1:248345/93‑1 (MQ=255)
                                                          gAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCa                              >  2:3705100/1‑93 (MQ=255)
                                                                 aaTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTg                       >  2:5527853/1‑93 (MQ=255)
                                                                 aaTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTg                       <  1:1638998/93‑1 (MQ=255)
                                                                  aTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGa                      >  1:6024638/1‑93 (MQ=255)
                                                                  aTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGa                      >  2:605764/1‑93 (MQ=255)
                                                                   ttCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAg                     >  1:5786556/1‑93 (MQ=255)
                                                                   ttCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAg                     <  1:5758131/93‑1 (MQ=255)
                                                                      cGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAgcgc                  <  2:3662025/93‑1 (MQ=255)
                                                                      cGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAgcgc                  <  1:1862289/93‑1 (MQ=255)
                                                                       ggTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGcc                 <  1:5054618/93‑1 (MQ=255)
                                                                        gTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCa                >  1:5473457/1‑93 (MQ=255)
                                                                        gTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCa                <  1:4592140/93‑1 (MQ=255)
                                                                         tGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCAt               <  2:4218199/93‑1 (MQ=255)
                                                                          ggggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATc              >  1:111180/1‑93 (MQ=255)
                                                                              ccATATAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacc          <  1:4236851/93‑1 (MQ=255)
                                                                              ccATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacc          >  2:625340/1‑93 (MQ=255)
                                                                              caaTACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacc          <  2:1512005/91‑1 (MQ=255)
                                                                               cATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacca         <  2:1524909/93‑1 (MQ=255)
                                                                               cATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacca         <  2:1717149/93‑1 (MQ=255)
                                                                                agaCAGTTTAGGCCCGGAGAACACGTAAAACTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCTCCATCCACCAg        <  1:2036451/91‑1 (MQ=255)
                                                                                  aCAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCa      <  1:3338757/93‑1 (MQ=255)
                                                                                   cAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCAc     <  1:71444/93‑1 (MQ=255)
                                                                                     gTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACtt   <  2:5016235/93‑1 (MQ=255)
                                                                                     gTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACtt   <  2:3461602/93‑1 (MQ=255)
                                                                                     gTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACtt   <  1:293798/93‑1 (MQ=255)
                                                                                      tttACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACttt  >  2:6175609/1‑93 (MQ=255)
                                                                                      tttACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACttt  <  1:2931929/93‑1 (MQ=255)
                                                                                          |                                                                                        
ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT  >  NC_000913/1757784‑1757962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGCGC  >  NC_000913/1757781‑1757967
                                                                                             |                                                                                             
ATCATAGAGCCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTAC                                                                                               <  SRR4302536.4239550/94‑1 (MQ=60)
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTAC                                                                                               <  SRR4302536.5273947/94‑1 (MQ=60)
 TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACG                                                                                              <  SRR4302536.925578/94‑1 (MQ=60)
   ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCC                                                                                            >  SRR4302536.4665750/1‑94 (MQ=60)
    TAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCC                                                                                           <  SRR4302536.6532862/94‑1 (MQ=60)
     AGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCG                                                                                          >  SRR4302536.864476/1‑94 (MQ=60)
       AACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGA                                                                                        >  SRR4302536.331196/1‑94 (MQ=60)
        ACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAG                                                                                       <  SRR4302536.5174963/94‑1 (MQ=60)
          CGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAA                                                                                     >  SRR4302536.2756867/1‑94 (MQ=60)
           GCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAAC                                                                                    >  SRR4302536.1899620/1‑94 (MQ=60)
            CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGGCCGGAGAACA                                                                                   >  SRR4302536.360007/1‑94 (MQ=60)
            CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACC                                                                                   >  SRR4302536.1917425/1‑94 (MQ=60)
            CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACA                                                                                   >  SRR4302536.2625136/1‑94 (MQ=60)
            CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACA                                                                                   <  SRR4302536.2940188/94‑1 (MQ=60)
            CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACA                                                                                   <  SRR4302536.5671643/94‑1 (MQ=60)
            CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACA                                                                                   <  SRR4302536.1677063/94‑1 (MQ=60)
             CCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACAC                                                                                  <  SRR4302536.5157672/94‑1 (MQ=60)
             CCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACAC                                                                                  >  SRR4302536.4986796/1‑94 (MQ=60)
             CCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACAC                                                                                  >  SRR4302536.169642/1‑94 (MQ=60)
             CCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACAC                                                                                  <  SRR4302536.4753179/94‑1 (MQ=60)
              CCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACG                                                                                 <  SRR4302536.487167/94‑1 (MQ=60)
              CCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACG                                                                                 <  SRR4302536.2713257/94‑1 (MQ=60)
                CTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTA                                                                               >  SRR4302536.2723574/1‑94 (MQ=60)
                CTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTA                                                                               >  SRR4302536.4735380/1‑94 (MQ=60)
                 TTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAA                                                                              >  SRR4302536.2104346/1‑94 (MQ=60)
                 TTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAA                                                                              >  SRR4302536.3182608/1‑94 (MQ=60)
                  TCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAA                                                                             <  SRR4302536.4627578/94‑1 (MQ=60)
                   CCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAA                                                                            <  SRR4302536.2453444/94‑1 (MQ=60)
                   CCCACGGCAGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAA                                                                            <  SRR4302536.6649168/94‑1 (MQ=60)
                   CCCAAGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAA                                                                            >  SRR4302536.4426902/1‑94 (MQ=60)
                    CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAG                                                                           >  SRR4302536.1976471/1‑94 (MQ=60)
                    CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAG                                                                           <  SRR4302536.2671583/94‑1 (MQ=60)
                      ACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTC                                                                         <  SRR4302536.5713890/94‑1 (MQ=60)
                      ACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTC                                                                         <  SRR4302536.3807839/94‑1 (MQ=60)
                        GGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGC                                                                       <  SRR4302536.3398943/94‑1 (MQ=60)
                        GGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGC                                                                       >  SRR4302536.2868794/1‑94 (MQ=60)
                          CGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAA                                                                     <  SRR4302536.1220782/94‑1 (MQ=60)
                           GGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAT                                                                    <  SRR4302536.1431959/94‑1 (MQ=60)
                             CATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCC                                                                  >  SRR4302536.699594/1‑94 (MQ=60)
                             CATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCC                                                                  <  SRR4302536.5748457/94‑1 (MQ=60)
                             CATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCC                                                                  >  SRR4302536.1580986/1‑94 (MQ=60)
                             CATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCC                                                                  >  SRR4302536.3482968/1‑94 (MQ=60)
                             CATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCC                                                                  >  SRR4302536.3250825/1‑94 (MQ=60)
                              ATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCA                                                                 >  SRR4302536.4682571/1‑94 (MQ=60)
                              ATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCA                                                                 >  SRR4302536.1534231/1‑94 (MQ=60)
                               TCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAG                                                                >  SRR4302536.1558834/1‑94 (MQ=60)
                               TCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAG                                                                >  SRR4302536.4429028/1‑94 (MQ=60)
                               TCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATAAAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAG                                                                <  SRR4302536.3256839/94‑1 (MQ=60)
                                CTCCTGCAACAAGTCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGTCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGC                                                               <  SRR4302536.2322396/94‑1 (MQ=60)
                                 TCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCG                                                              <  SRR4302536.1920927/94‑1 (MQ=60)
                                       AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                        <  SRR4302536.429494/94‑1 (MQ=60)
                                       AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                        >  SRR4302536.2151685/1‑94 (MQ=60)
                                        ACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAC                                                       >  SRR4302536.2243960/1‑94 (MQ=60)
                                        ACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAC                                                       <  SRR4302536.6494515/94‑1 (MQ=60)
                                        ACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAC                                                       >  SRR4302536.6353719/1‑94 (MQ=60)
                                         CAAGGCTTCTTTCACATAAAGACTGCCAATCCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACA                                                      <  SRR4302536.2807435/94‑1 (MQ=60)
                                         CAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACA                                                      >  SRR4302536.1973181/1‑94 (MQ=60)
                                         CAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACA                                                      <  SRR4302536.2637465/94‑1 (MQ=60)
                                         CAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACA                                                      >  SRR4302536.132362/1‑94 (MQ=60)
                                         CAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACA                                                      <  SRR4302536.5162753/94‑1 (MQ=60)
                                         CAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGGTTACGCCCGGAGAACACGTAAAAGACGCAATCCAGCGCCTGAACA                                                      >  SRR4302536.6470234/1‑94 (MQ=60)
                                          AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                     <  SRR4302536.4120530/94‑1 (MQ=60)
                                          AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                     >  SRR4302536.2990341/1‑94 (MQ=60)
                                          AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                     <  SRR4302536.4212599/94‑1 (MQ=60)
                                           AGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATC                                                    >  SRR4302536.1568965/1‑94 (MQ=60)
                                           AGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATC                                                    <  SRR4302536.3850887/94‑1 (MQ=60)
                                           AGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATC                                                    >  SRR4302536.5389374/1‑94 (MQ=60)
                                            GGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCC                                                   >  SRR4302536.1717744/1‑94 (MQ=60)
                                            GGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCC                                                   >  SRR4302536.897581/1‑94 (MQ=60)
                                             GCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCA                                                  <  SRR4302536.6668360/94‑1 (MQ=60)
                                             GCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCA                                                  >  SRR4302536.4878850/1‑94 (MQ=60)
                                             GCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCA                                                  <  SRR4302536.4907930/94‑1 (MQ=60)
                                              CTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAC                                                 >  SRR4302536.4042649/1‑94 (MQ=60)
                                              CTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAC                                                 >  SRR4302536.6793244/1‑94 (MQ=60)
                                              CTTCTGTCACAGAAAGAAAGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAC                                                 <  SRR4302536.4731658/94‑1 (MQ=60)
                                               TTCTTGCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACC                                                <  SRR4302536.6090562/94‑1 (MQ=60)
                                                TCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCG                                               <  SRR4302536.2985144/94‑1 (MQ=60)
                                                TCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCG                                               >  SRR4302536.3593605/1‑94 (MQ=60)
                                                 CTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGG                                              >  SRR4302536.407542/1‑94 (MQ=60)
                                                 CTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGG                                              >  SRR4302536.1894972/1‑94 (MQ=60)
                                                      ACATAAAGAATGCCAATTCCGGTGGGGGCATACAGATTACGCCCGGAGAACACGTAAAAATCGCAAAAAAGGGCCTGAACATCCACCcgcagac                                         >  SRR4302536.3271954/1‑87 (MQ=52)
                                                      ACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGAT                                         >  SRR4302536.3510124/1‑94 (MQ=60)
                                                      ACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGAT                                         <  SRR4302536.4062212/94‑1 (MQ=60)
                                                       CATAAAGAATGCCAATTCCGGTGTGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGGAATCCAGCGCCTGAtcatacatctgctgatt                                        >  SRR4302536.6042907/1‑77 (MQ=60)
                                                       CATAAAGAATGCCAATTCCGGTGGTGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                        <  SRR4302536.2741589/94‑1 (MQ=60)
                                                       CATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                        >  SRR4302536.23284/1‑94 (MQ=60)
                                                       CATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                        >  SRR4302536.4591815/1‑94 (MQ=60)
                                                       CATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                        >  SRR4302536.3018882/1‑94 (MQ=60)
                                                       CATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                        >  SRR4302536.327119/1‑94 (MQ=60)
                                                        ATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGC                                       >  SRR4302536.154389/1‑94 (MQ=60)
                                                         TAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCA                                      <  SRR4302536.5973854/94‑1 (MQ=60)
                                                          AAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAT                                     <  SRR4302536.6396182/94‑1 (MQ=60)
                                                          AAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAT                                     >  SRR4302536.2645338/1‑94 (MQ=60)
                                                          AAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAT                                     >  SRR4302536.3914776/1‑94 (MQ=60)
                                                           AAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATC                                    >  SRR4302536.6789319/1‑94 (MQ=60)
                                                            AGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCC                                   >  SRR4302536.1799670/1‑94 (MQ=60)
                                                            AGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCA                                   >  SRR4302536.1517825/1‑94 (MQ=60)
                                                             GAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAC                                  <  SRR4302536.254977/94‑1 (MQ=60)
                                                              AATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAGCATCCACCGGATGATGCATCACC                                 <  SRR4302536.3260589/94‑1 (MQ=60)
                                                                  CCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCT                             <  SRR4302536.1947741/94‑1 (MQ=60)
                                                                  CCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCT                             >  SRR4302536.2737299/1‑94 (MQ=60)
                                                                    AATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGA                           >  SRR4302536.6204267/1‑94 (MQ=60)
                                                                    AATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGA                           >  SRR4302536.1408161/1‑94 (MQ=60)
                                                                    AATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGA                           <  SRR4302536.1686711/94‑1 (MQ=60)
                                                                     ATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAG                          >  SRR4302536.5958810/1‑94 (MQ=60)
                                                                      TTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGC                         <  SRR4302536.5929434/94‑1 (MQ=60)
                                                                      TGCCGGTGGGGCCATACAGTTTCCGCCGGGAGAACACGTAAAAGTCGCAACCCAGCGCCTGAACACCCACCGGATGATGCATCACCGCCTGAGC                         <  SRR4302536.3598200/94‑1 (MQ=60)
                                                                       TCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCG                        <  SRR4302536.2567033/94‑1 (MQ=60)
                                                                        CCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAAATCCACCGGATGATGCATCACCGCCTGAGCGC                       >  SRR4302536.6208272/1‑94 (MQ=60)
                                                                         CGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCC                      <  SRR4302536.1918375/94‑1 (MQ=60)
                                                                         CGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCC                      >  SRR4302536.2430151/1‑94 (MQ=60)
                                                                          GGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCA                     >  SRR4302536.5636969/1‑94 (MQ=60)
                                                                          GGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCA                     >  SRR4302536.5139111/1‑94 (MQ=60)
                                                                          GGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCA                     <  SRR4302536.5206043/94‑1 (MQ=60)
                                                                           GTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCAT                    <  SRR4302536.4729998/94‑1 (MQ=60)
                                                                            TGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATC                   >  SRR4302536.114116/1‑94 (MQ=60)
                                                                             GGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCC                  >  SRR4302536.5505761/1‑94 (MQ=60)
                                                                              GGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCA                 <  SRR4302536.5640127/94‑1 (MQ=60)
                                                                               GGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCAC                >  SRR4302536.6034300/1‑94 (MQ=60)
                                                                                 CCATATAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCA              <  SRR4302536.4362967/94‑1 (MQ=60)
                                                                                  CATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAG             >  SRR4302536.4116299/1‑94 (MQ=60)
                                                                                   CTACAGTTTACGCCCGGAGACCACGTAAAAGTCCCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGC            <  SRR4302536.2903993/94‑1 (MQ=60)
                                                                                   ATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGC            >  SRR4302536.5946842/1‑94 (MQ=60)
                                                                                   AGACAGTTTAGGCCCGGAGAACACGTAAAACTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCTCCATCCACCAGC            <  SRR4302536.2097774/94‑1 (MQ=60)
                                                                                    TACAGTTTACGCCCGGAGAACACGTAAAAGTTGCAATCCAGCGCCTGAACATCCACCGGCTGATGCATCACCGCCTGAGCGCCATCCACCAGCC           >  SRR4302536.816759/1‑94 (MQ=60)
                                                                                     ACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCAC          <  SRR4302536.3437667/94‑1 (MQ=60)
                                                                                     ACAGTTTACGCCCGGAGAACACGTAAAAGACGCAATCCAGCGCCTGTACATCCACAGGATGATGCATTACCGCCTGAGCGCCATCCACCAGCAC          >  SRR4302536.4946301/1‑94 (MQ=60)
                                                                                      CAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACT         <  SRR4302536.73383/94‑1 (MQ=60)
                                                                                        GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGGGCGCCATCCACCAGCACTTT       <  SRR4302536.6764135/94‑1 (MQ=60)
                                                                                        GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT       <  SRR4302536.301608/94‑1 (MQ=60)
                                                                                        GTGTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT       <  SRR4302536.2530217/94‑1 (MQ=60)
                                                                                         TTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTT      <  SRR4302536.3018441/94‑1 (MQ=60)
                                                                                           TACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGC    <  SRR4302536.5567232/94‑1 (MQ=60)
                                                                                           TACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGC    <  SRR4302536.5198923/94‑1 (MQ=60)
                                                                                             CGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCGTGAGCGCCATCCACCAGCACTTTTGCGC  <  SRR4302536.886191/94‑1 (MQ=60)
                                                                                             CGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGCGC  >  SRR4302536.3032985/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGCGC  >  NC_000913/1757781‑1757967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑