Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,827,618 A→G R47R (CGT→CGC)  b1746 ← putative aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,6180AG100.0% 618.0 / NA 191R47R (CGT→CGC) b1746putative aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (105/86);  total (105/86)

Only 100 of 198 total aligned reads displayed.

GGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCAC  >  NC_000913/1827528‑1827707
                                                                                          |                                                                                         
 gCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCgg                                                                                        >  1:5774194/1‑93 (MQ=255)
  cTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCggg                                                                                       >  2:3968278/1‑93 (MQ=255)
   tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGc                                                                                      <  1:4607270/93‑1 (MQ=255)
   tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGc                                                                                      <  2:1543194/93‑1 (MQ=255)
   tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCAGCGCCCAGCGCGGAACCGCCGCGCGGGc                                                                                      >  2:5738326/1‑93 (MQ=255)
   tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGc                                                                                      >  2:1530030/1‑93 (MQ=255)
     tATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTg                                                                                    >  2:2186248/1‑93 (MQ=255)
       ttGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGcc                                                                                  >  2:2877355/1‑93 (MQ=255)
        tGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGccc                                                                                 >  1:845070/1‑93 (MQ=255)
          cTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGa                                                                               >  2:19443/1‑93 (MQ=255)
           tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGAc                                                                              <  1:1192291/93‑1 (MQ=255)
           tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGAc                                                                              >  2:1677073/1‑93 (MQ=255)
            ttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACa                                                                             >  1:178250/1‑93 (MQ=255)
             tCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACaa                                                                            <  1:1950262/93‑1 (MQ=255)
             tCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACaa                                                                            <  1:1366188/93‑1 (MQ=255)
               cagcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGc                                                                          >  1:6446837/1‑93 (MQ=255)
                agcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGcc                                                                         >  2:6020272/1‑93 (MQ=255)
                agcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGcc                                                                         <  2:6656900/93‑1 (MQ=255)
                agcagTGCGCCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGcc                                                                         <  1:4105461/93‑1 (MQ=255)
                 gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGACCGACCAGCCt                                                                        >  1:4318566/1‑93 (MQ=255)
                   agTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGc                                                                      >  1:4385949/1‑93 (MQ=255)
                      gCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCg                                                                   <  2:6671574/93‑1 (MQ=255)
                      gCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCg                                                                   <  1:4083504/93‑1 (MQ=255)
                        ggCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGAc                                                                 <  2:2846468/93‑1 (MQ=255)
                        ggCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGAc                                                                 >  2:2477074/1‑93 (MQ=255)
                         gCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGAcc                                                                <  1:2485257/93‑1 (MQ=255)
                          cAAAGCGGTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCt                                                               <  1:1319228/93‑1 (MQ=255)
                           aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTg                                                              >  1:4803141/1‑93 (MQ=255)
                           aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGACCAGCGCGGAAACGCCGCGCGGGCGGCCCGACAAGTCTGCTCGACCTg                                                              >  1:3285629/1‑93 (MQ=255)
                            aaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                             >  2:851533/1‑93 (MQ=255)
                            aaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                             <  2:4509796/93‑1 (MQ=255)
                            aaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                             >  2:6371601/1‑93 (MQ=255)
                            aaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                             <  2:322147/93‑1 (MQ=255)
                              gCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGc                                                           <  1:5081263/93‑1 (MQ=255)
                              gCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGc                                                           >  1:5436758/1‑93 (MQ=255)
                               cGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                          <  1:5229519/93‑1 (MQ=255)
                               cGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                          >  1:5806966/1‑93 (MQ=255)
                               cGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                          <  1:6622444/93‑1 (MQ=255)
                                gTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcgg                                                         >  2:5776198/1‑93 (MQ=255)
                                   cGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                      <  1:4470773/93‑1 (MQ=255)
                                      cagcGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcgg                                                   <  1:6081271/90‑1 (MQ=255)
                                      cAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcgg                                                   >  2:5579450/1‑93 (MQ=255)
                                        aCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggca                                                 >  2:5529699/1‑93 (MQ=255)
                                        aCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggca                                                 <  2:6734482/93‑1 (MQ=255)
                                         cGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                >  2:3277441/1‑93 (MQ=255)
                                         cGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                >  1:907390/1‑93 (MQ=255)
                                          ggCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCggcatcggcatc                                               <  2:1573700/93‑1 (MQ=255)
                                           gCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                              >  2:496022/1‑93 (MQ=255)
                                           gCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                              >  1:972284/1‑93 (MQ=255)
                                            cATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                             <  2:3823879/93‑1 (MQ=255)
                                             aTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                            <  1:237476/93‑1 (MQ=255)
                                               gACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgc                                          >  1:3579318/1‑93 (MQ=255)
                                                aCGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcc                                         >  2:380605/1‑93 (MQ=255)
                                                 cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                        <  2:4242322/93‑1 (MQ=255)
                                                 cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                        <  1:407449/93‑1 (MQ=255)
                                                 cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                        <  1:3942783/93‑1 (MQ=255)
                                                 cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                        >  2:5299753/1‑93 (MQ=255)
                                                 cGTTCACCAAATGAAGGCCGCGCCCGGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                        <  1:3514257/93‑1 (MQ=255)
                                                  gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                       <  2:1795843/93‑1 (MQ=255)
                                                  gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                       >  1:3756092/1‑93 (MQ=255)
                                                  gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                       >  2:5403241/1‑93 (MQ=255)
                                                    tCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                     <  2:137404/93‑1 (MQ=255)
                                                     cAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                    <  1:3857846/93‑1 (MQ=255)
                                                       gCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCAt                                  >  2:5918333/1‑93 (MQ=255)
                                                        cAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATa                                 <  2:2054445/93‑1 (MQ=255)
                                                          aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                               <  1:2799317/93‑1 (MQ=255)
                                                           aTGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAaca                              >  1:116344/1‑93 (MQ=255)
                                                           aTGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAaca                              >  1:6727330/1‑93 (MQ=255)
                                                            tGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAacac                             >  1:5542521/1‑93 (MQ=255)
                                                            agAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAacac                             <  2:6132920/92‑1 (MQ=255)
                                                             gAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                            >  1:5818610/1‑93 (MQ=255)
                                                              aaaGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                           <  2:212302/93‑1 (MQ=255)
                                                               aaGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTc                          >  1:965898/1‑93 (MQ=255)
                                                                aGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                         >  1:2194094/1‑93 (MQ=255)
                                                                aGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                         <  1:6218602/93‑1 (MQ=255)
                                                                aGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                         >  2:3082939/1‑93 (MQ=255)
                                                                  ccGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGcc                       <  2:3687578/93‑1 (MQ=255)
                                                                   cgcgCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                      <  2:2658641/93‑1 (MQ=255)
                                                                   cgcgCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                      >  2:5230789/1‑93 (MQ=255)
                                                                    gcgcCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                     >  1:3700011/1‑93 (MQ=255)
                                                                      gcCCAGCGCGTAAACGCGGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                   <  1:1672651/93‑1 (MQ=255)
                                                                      gcCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                   <  1:458720/93‑1 (MQ=255)
                                                                       cccAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                  <  2:1243444/93‑1 (MQ=255)
                                                                       cccAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                  <  1:133861/93‑1 (MQ=255)
                                                                       cccAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                  >  1:2242248/1‑93 (MQ=255)
                                                                        ccAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                 >  1:5853269/1‑93 (MQ=255)
                                                                        ccAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                 <  1:6469648/93‑1 (MQ=255)
                                                                         cAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                <  2:4076381/93‑1 (MQ=255)
                                                                          aGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCg               >  1:6147811/1‑93 (MQ=255)
                                                                          aGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCg               >  2:4673624/1‑93 (MQ=255)
                                                                           gcgcGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCgg              >  2:242416/1‑93 (MQ=255)
                                                                             gcgGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt            <  1:4870759/93‑1 (MQ=255)
                                                                             gcgGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt            <  2:6207746/93‑1 (MQ=255)
                                                                             gcgGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt            <  1:2337965/93‑1 (MQ=255)
                                                                             gcgGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt            <  2:1431013/93‑1 (MQ=255)
                                                                               ggAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa          <  1:1458936/93‑1 (MQ=255)
                                                                                   aCGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt      >  2:4384423/1‑93 (MQ=255)
                                                                                    cgccgcGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt     <  1:5042658/93‑1 (MQ=255)
                                                                                    cgccgcGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt     >  1:1921939/1‑93 (MQ=255)
                                                                                    cgccgcGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt     >  2:3548477/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCAC  >  NC_000913/1827528‑1827707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTTCACACGC  >  NC_000913/1827525‑1827711
                                                                                             |                                                                                              
TTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCG                                                                                                <  SRR4302536.769856/94‑1 (MQ=60)
 TCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGC                                                                                               >  SRR4302536.2843774/1‑94 (MQ=60)
  CGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCG                                                                                              >  SRR4302536.1800220/1‑94 (MQ=60)
   GGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGG                                                                                             >  SRR4302536.5946010/1‑94 (MQ=60)
   GGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGG                                                                                             >  SRR4302536.721686/1‑94 (MQ=60)
   GGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGG                                                                                             >  SRR4302536.5982748/1‑94 (MQ=60)
     CTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGC                                                                                           >  SRR4302536.5482235/1‑94 (MQ=60)
     CTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGC                                                                                           <  SRR4302536.4698615/94‑1 (MQ=60)
     CTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGC                                                                                           >  SRR4302536.1661132/1‑94 (MQ=60)
      TTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCT                                                                                          <  SRR4302536.356082/94‑1 (MQ=60)
      TTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCT                                                                                          <  SRR4302536.4745653/94‑1 (MQ=60)
        TATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGCGCTGC                                                                                        >  SRR4302536.6339213/1‑94 (MQ=60)
          TTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAATGCCGCGCGGGCTGCCC                                                                                      >  SRR4302536.3644948/1‑94 (MQ=60)
          TTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCC                                                                                      >  SRR4302536.869913/1‑94 (MQ=60)
          TTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGAGGGCTGCCC                                                                                      >  SRR4302536.434820/1‑94 (MQ=60)
            GCTTTCCAGCAGTGCCGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGA                                                                                    <  SRR4302536.4891533/94‑1 (MQ=60)
             CTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGAC                                                                                   >  SRR4302536.3863201/1‑94 (MQ=60)
              TTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACA                                                                                  >  SRR4302536.182954/1‑94 (MQ=60)
              TTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACA                                                                                  <  SRR4302536.1228137/94‑1 (MQ=60)
                TCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAG                                                                                <  SRR4302536.2008907/94‑1 (MQ=60)
                TCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAG                                                                                <  SRR4302536.1406914/94‑1 (MQ=60)
                 CCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGC                                                                               >  SRR4302536.6639435/1‑94 (MQ=60)
                  CAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCC                                                                              <  SRR4302536.3699844/94‑1 (MQ=60)
                   AGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCT                                                                             >  SRR4302536.1892901/1‑94 (MQ=60)
                   AGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGACCGACCAGCCT                                                                             >  SRR4302536.4447864/1‑94 (MQ=60)
                   AGCAGTGCGCCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCT                                                                             <  SRR4302536.4227263/94‑1 (MQ=60)
                    GCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTG                                                                            <  SRR4302536.2766954/94‑1 (MQ=60)
                     CAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGC                                                                           >  SRR4302536.4516922/1‑94 (MQ=60)
                         GCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGA                                                                       <  SRR4302536.4204562/94‑1 (MQ=60)
                            GCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCT                                                                    <  SRR4302536.2560241/94‑1 (MQ=60)
                             CAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTG                                                                   >  SRR4302536.6645723/1‑94 (MQ=60)
                             CAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTG                                                                   >  SRR4302536.4947700/1‑94 (MQ=60)
                             CAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTG                                                                   >  SRR4302536.1324610/1‑94 (MQ=60)
                             CAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGACCAGCGCGGAAACGCCGCGCGGGCGGCCCGACAAGTCTGCTCGACCTG                                                                   >  SRR4302536.3382273/1‑94 (MQ=60)
                             CAAAGCGGTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTG                                                                   <  SRR4302536.1358609/94‑1 (MQ=60)
                               AAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAG                                                                 >  SRR4302536.4976167/1‑94 (MQ=60)
                                AGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGC                                                                >  SRR4302536.5599336/1‑94 (MQ=60)
                                AGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGAGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGG                                                                >  SRR4302536.5255398/1‑94 (MQ=60)
                                 GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCG                                                               <  SRR4302536.297922/94‑1 (MQ=60)
                                 GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCG                                                               <  SRR4302536.5233323/94‑1 (MQ=60)
                                 GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCG                                                               >  SRR4302536.5979909/1‑94 (MQ=60)
                                  CGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGG                                                              <  SRR4302536.5385341/94‑1 (MQ=60)
                                  CGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGG                                                              <  SRR4302536.6819380/94‑1 (MQ=60)
                                     TCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCAT                                                           <  SRR4302536.6295570/94‑1 (MQ=60)
                                      CGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATC                                                          <  SRR4302536.5025803/94‑1 (MQ=60)
                                      CGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATC                                                          <  SRR4302536.4605074/94‑1 (MQ=60)
                                       GACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCG                                                         <  SRR4302536.2268297/94‑1 (MQ=60)
                                        ACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGG                                                        >  SRR4302536.5995254/1‑94 (MQ=60)
                                         CAGCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGC                                                       <  SRR4302536.6262667/94‑1 (MQ=60)
                                           ACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCAT                                                     >  SRR4302536.934122/1‑94 (MQ=60)
                                           ACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGACCGGCATCGGCAT                                                     >  SRR4302536.1147613/1‑94 (MQ=60)
                                             GGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCA                                                   >  SRR4302536.1000974/1‑94 (MQ=60)
                                             GGCATGACGTCCAGCAAATGAAAGCCGCGCCGAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCA                                                   <  SRR4302536.6678106/94‑1 (MQ=60)
                                               CATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATT                                                 >  SRR4302536.6880638/1‑94 (MQ=60)
                                               CATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCCGCATCACT                                                 >  SRR4302536.242601/1‑94 (MQ=60)
                                               CAGGACGTTCAGCAAATGAAGACCGCGCCAAGCGCGGAAACGCCGCGCGGTCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATT                                                 <  SRR4302536.526384/94‑1 (MQ=60)
                                                ATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTG                                                <  SRR4302536.4184089/94‑1 (MQ=60)
                                                ATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTG                                                <  SRR4302536.243785/94‑1 (MQ=60)
                                                ATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTG                                                <  SRR4302536.398883/94‑1 (MQ=60)
                                                 TGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGC                                               >  SRR4302536.3686353/1‑94 (MQ=60)
                                                  GACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCT                                              >  SRR4302536.644206/1‑94 (MQ=60)
                                                   ACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCT                                             >  SRR4302536.2127991/1‑94 (MQ=60)
                                                   ACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCT                                             <  SRR4302536.5665499/94‑1 (MQ=60)
                                                   ACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCT                                             >  SRR4302536.932457/1‑94 (MQ=60)
                                                    CGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTT                                            >  SRR4302536.3868301/1‑94 (MQ=60)
                                                    CGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTT                                            <  SRR4302536.4059909/94‑1 (MQ=60)
                                                    CGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTT                                            <  SRR4302536.418199/94‑1 (MQ=60)
                                                    CGTTCACCAAATGAAGGCCGCGCCCGGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTT                                            <  SRR4302536.3619200/94‑1 (MQ=60)
                                                     GTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTG                                           <  SRR4302536.999620/94‑1 (MQ=60)
                                                      TTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGC                                          >  SRR4302536.481778/1‑94 (MQ=60)
                                                       TCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCC                                         >  SRR4302536.1378213/1‑94 (MQ=60)
                                                       TCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCC                                         >  SRR4302536.2408832/1‑94 (MQ=60)
                                                        CAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCA                                        <  SRR4302536.4597369/94‑1 (MQ=60)
                                                        CAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCA                                        <  SRR4302536.6186034/94‑1 (MQ=60)
                                                        CAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCA                                        <  SRR4302536.3972660/94‑1 (MQ=60)
                                                             AATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACA                                   >  SRR4302536.119420/1‑94 (MQ=60)
                                                             AATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACA                                   >  SRR4302536.6927203/1‑94 (MQ=60)
                                                             AATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACA                                   <  SRR4302536.2882340/94‑1 (MQ=60)
                                                              ATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAC                                  >  SRR4302536.5707683/1‑94 (MQ=60)
                                                               TGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACC                                 <  SRR4302536.5200526/94‑1 (MQ=60)
                                                               TGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACC                                 >  SRR4302536.5991944/1‑94 (MQ=60)
                                                                 AAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑C                              >  SRR4302536.994425/1‑94 (MQ=60)
                                                                  AAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CG                             >  SRR4302536.149677/1‑94 (MQ=60)
                                                                  AAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CG                             >  SRR4302536.2260295/1‑94 (MQ=60)
                                                                  AAGCCGCGCCCAGCGCGGAAACACCACGCGGGCTGCCCTACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATCACACCT‑CG                             >  SRR4302536.6281944/1‑94 (MQ=60)
                                                                   AGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGC                            <  SRR4302536.6403912/94‑1 (MQ=60)
                                                                     CCGCGCCCCGCGCGGCAACGCCCCGCGGGATGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGGCATCACACCTCCGCC                           >  SRR4302536.123618/1‑94 (MQ=60)
                                                                      CGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCG                         >  SRR4302536.3810649/1‑94 (MQ=60)
                                                                       GCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGA                        >  SRR4302536.3927976/1‑94 (MQ=60)
                                                                         GCCCAGCGCGTAAACGCGGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATA                      <  SRR4302536.1721161/94‑1 (MQ=60)
                                                                         GCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATA                      <  SRR4302536.470721/94‑1 (MQ=60)
                                                                         GCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATA                      >  SRR4302536.2310103/1‑94 (MQ=60)
                                                                          CCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATAC                     >  SRR4302536.6027659/1‑94 (MQ=60)
                                                                          CCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATAC                     <  SRR4302536.137377/94‑1 (MQ=60)
                                                                           CCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACC                    <  SRR4302536.4745493/94‑1 (MQ=60)
                                                                           CCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACC                    <  SRR4302536.4214376/94‑1 (MQ=60)
                                                                           CCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACC                    <  SRR4302536.6662747/94‑1 (MQ=60)
                                                                            CAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCG                   >  SRR4302536.6330968/1‑94 (MQ=60)
                                                                             AGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGG                  >  SRR4302536.4505648/1‑94 (MQ=60)
                                                                                GCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATT               <  SRR4302536.5017303/94‑1 (MQ=60)
                                                                                GCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATT               <  SRR4302536.2408894/94‑1 (MQ=60)
                                                                                  GGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTAC             <  SRR4302536.1502119/94‑1 (MQ=60)
                                                                                  GGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTAC             >  SRR4302536.4204234/1‑94 (MQ=60)
                                                                                   GAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACG            <  SRR4302536.1394017/94‑1 (MQ=60)
                                                                                      ACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTT         >  SRR4302536.1979755/1‑94 (MQ=60)
                                                                                      ACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTT         >  SRR4302536.5896532/1‑94 (MQ=60)
                                                                                       CGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTTC        <  SRR4302536.5193785/94‑1 (MQ=60)
                                                                                        GCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTTCA       >  SRR4302536.2004779/1‑94 (MQ=60)
                                                                                          CGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTTCACA     <  SRR4302536.2529266/94‑1 (MQ=60)
                                                                                            CGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTTCACACG   >  SRR4302536.1605593/1‑94 (MQ=60)
                                                                                             GCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTTCACACGC  >  SRR4302536.4418296/1‑94 (MQ=60)
                                                                                             |                                                                                              
TTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT‑CGCCCGATACCGGATTACGCTTCACACGC  >  NC_000913/1827525‑1827711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑