Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,865,007 A→G T26A (ACC→GCC)  yeaG → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,0070AG99.1% 683.4 / ‑3.0 212T26A (ACC→GCC) yeaGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (119/91);  total (120/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01

Only 100 of 214 total aligned reads displayed.

AAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGC  >  NC_000913/1864917‑1865095
                                                                                          |                                                                                        
aaaGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGcc                                                                                        <  2:5267847/93‑1 (MQ=255)
aaaGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGcc                                                                                        <  2:3045270/93‑1 (MQ=255)
  aGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCAc                                                                                      >  2:4138037/1‑93 (MQ=255)
  aGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCAc                                                                                      >  1:6356681/1‑93 (MQ=255)
   ggACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACt                                                                                     <  1:2409021/93‑1 (MQ=255)
   agACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACt                                                                                     <  2:6438682/92‑1 (MQ=255)
      cGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGGCACTTGt                                                                                  >  2:1170469/1‑93 (MQ=255)
      cGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGt                                                                                  <  2:6071402/93‑1 (MQ=255)
      cGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGt                                                                                  >  2:3456404/1‑93 (MQ=255)
       ggCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTc                                                                                 <  1:741162/93‑1 (MQ=255)
          aTACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGc                                                                              <  2:5150090/93‑1 (MQ=255)
          aTACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGc                                                                              >  2:6362664/1‑93 (MQ=255)
          aTACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGc                                                                              >  2:3312920/1‑93 (MQ=255)
           tACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCa                                                                             >  2:5752130/1‑93 (MQ=255)
           tACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCa                                                                             >  2:5344037/1‑93 (MQ=255)
             ccATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAg                                                                           <  2:1794924/93‑1 (MQ=255)
              cATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGCTTCTTGCCACTTGTCGCCAAGa                                                                          >  2:3929919/1‑93 (MQ=255)
               aTGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAt                                                                         <  1:2017543/93‑1 (MQ=255)
               aTGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAt                                                                         >  1:6031852/1‑93 (MQ=255)
                tGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATc                                                                        <  2:3517727/93‑1 (MQ=255)
                tGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATc                                                                        >  2:6284730/1‑93 (MQ=255)
                tGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATc                                                                        >  2:5203099/1‑93 (MQ=255)
                tGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATc                                                                        >  1:26263/1‑93 (MQ=255)
                tGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATc                                                                        >  1:2264839/1‑93 (MQ=255)
                 gAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATcc                                                                       >  2:3230675/1‑92 (MQ=255)
                 gAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCg                                                                       >  2:2835960/1‑93 (MQ=255)
                  aaTATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGc                                                                      >  1:5121445/1‑93 (MQ=255)
                   atatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCa                                                                     <  2:2366812/93‑1 (MQ=255)
                    tatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAg                                                                    <  2:2597680/93‑1 (MQ=255)
                      tatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTg                                                                  >  2:54686/1‑93 (MQ=255)
                       atTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGc                                                                 <  1:1492484/93‑1 (MQ=255)
                       atTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGc                                                                 <  2:152091/93‑1 (MQ=255)
                        ttCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCt                                                                >  1:4941936/1‑93 (MQ=255)
                          cGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTa                                                              <  2:2384512/93‑1 (MQ=255)
                            aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATg                                                            >  2:1947169/1‑93 (MQ=255)
                            aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATg                                                            <  1:1280619/93‑1 (MQ=255)
                            aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATg                                                            >  2:2579740/1‑93 (MQ=255)
                             tCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGc                                                           >  1:2924141/1‑93 (MQ=255)
                             tCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGc                                                           >  2:4400867/1‑93 (MQ=255)
                              cACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGcc                                                          <  1:3706416/93‑1 (MQ=255)
                               aCTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCa                                                         >  2:857362/1‑93 (MQ=255)
                                cTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCaa                                                        >  1:383106/1‑93 (MQ=255)
                                cTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCaa                                                        <  2:5401126/93‑1 (MQ=255)
                                 tATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAc                                                       >  1:6515925/1‑93 (MQ=255)
                                   tCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAcgc                                                     >  1:2110431/1‑93 (MQ=255)
                                   tCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAcgc                                                     <  1:678572/93‑1 (MQ=255)
                                     gCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCgg                                                   >  2:3665719/1‑93 (MQ=255)
                                        aGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTg                                                <  2:4646438/93‑1 (MQ=255)
                                         gCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGa                                               <  1:3126166/93‑1 (MQ=255)
                                         gCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGa                                               <  1:4098014/93‑1 (MQ=255)
                                           gATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCTGCTGAGc                                             >  2:312369/1‑93 (MQ=255)
                                           gATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGc                                             <  1:5534611/93‑1 (MQ=255)
                                               tGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCt                                         <  1:590453/93‑1 (MQ=255)
                                               tGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCt                                         >  2:225588/1‑93 (MQ=255)
                                               tGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCt                                         >  2:5869818/1‑93 (MQ=255)
                                                gAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTa                                        >  2:601329/1‑93 (MQ=255)
                                                 aaGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                       >  2:1744768/1‑93 (MQ=255)
                                                 aaGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                       <  2:1222854/93‑1 (MQ=255)
                                                 aaGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                       <  1:2803915/93‑1 (MQ=255)
                                                   gCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTg                                     <  1:6100769/93‑1 (MQ=255)
                                                     tGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAt                                   <  1:462134/93‑1 (MQ=255)
                                                       ccAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATgg                                 >  1:1099604/1‑93 (MQ=255)
                                                         aaGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCt                               <  1:6050638/93‑1 (MQ=255)
                                                            gACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATc                            <  2:1717106/93‑1 (MQ=255)
                                                            gACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATc                            <  1:5439064/93‑1 (MQ=255)
                                                            gACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATc                            <  1:3908875/93‑1 (MQ=255)
                                                              cGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCgg                          <  2:3185964/93‑1 (MQ=255)
                                                               gAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                         >  1:4812914/1‑93 (MQ=255)
                                                                  gagTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAg                      >  2:4802838/1‑93 (MQ=255)
                                                                   agTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGc                     >  2:3626903/1‑93 (MQ=255)
                                                                    gTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGcc                    >  2:492202/1‑93 (MQ=255)
                                                                    gTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGcc                    >  1:5818228/1‑93 (MQ=255)
                                                                     ttCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCt                   >  2:6692086/1‑93 (MQ=255)
                                                                     ttCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCt                   <  1:2468037/93‑1 (MQ=255)
                                                                      tCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTg                  <  1:5628738/93‑1 (MQ=255)
                                                                        acacTGCAGGAGCTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                >  2:1871609/1‑93 (MQ=255)
                                                                         cacTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCa               <  1:1809590/93‑1 (MQ=255)
                                                                         cacTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCa               >  1:1689992/1‑93 (MQ=255)
                                                                          acTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCAt              <  1:3435425/93‑1 (MQ=255)
                                                                          acTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCAt              <  1:2739734/93‑1 (MQ=255)
                                                                           cTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATg             >  1:2834031/1‑93 (MQ=255)
                                                                           cTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATg             >  1:1391712/1‑93 (MQ=255)
                                                                           cTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATg             <  1:1899430/93‑1 (MQ=255)
                                                                           cTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATg             >  1:3802602/1‑93 (MQ=255)
                                                                             gCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGt           <  2:96249/93‑1 (MQ=255)
                                                                             gCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGt           <  2:3353887/93‑1 (MQ=255)
                                                                             gCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGt           >  1:2076279/1‑93 (MQ=255)
                                                                              cAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTc          <  1:5376677/93‑1 (MQ=255)
                                                                               aGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCg         >  2:2736550/1‑93 (MQ=255)
                                                                               aGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCg         >  1:5877289/1‑93 (MQ=255)
                                                                                ggAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGa        <  1:835235/93‑1 (MQ=255)
                                                                                ggAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGa        <  1:5583911/93‑1 (MQ=255)
                                                                                ggAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGa        >  1:4510570/1‑93 (MQ=255)
                                                                                ggAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGa        >  1:1768683/1‑93 (MQ=255)
                                                                                  aGTTTCTTGCCTCTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa      <  1:1147871/93‑1 (MQ=255)
                                                                                  aGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa      <  2:232214/93‑1 (MQ=255)
                                                                                  aGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa      <  2:656904/93‑1 (MQ=255)
                                                                                    tttCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACa    <  2:4339150/93‑1 (MQ=255)
                                                                                    tttCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACa    <  1:4069488/93‑1 (MQ=255)
                                                                                    tttCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACa    <  1:3628903/93‑1 (MQ=255)
                                                                                          |                                                                                        
AAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGC  >  NC_000913/1864917‑1865095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGG  >  NC_000913/1864914‑1865100
                                                                                             |                                                                                             
ACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTG                                                                                               <  SRR4302536.995420/94‑1 (MQ=60)
    AAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCAC                                                                                           >  SRR4302536.6546055/1‑94 (MQ=60)
    AAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATAAGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCAC                                                                                           >  SRR4302536.5262925/1‑94 (MQ=60)
     AGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCGCACTGCAGGAGTTTCTTGCCACT                                                                                          >  SRR4302536.2692194/1‑94 (MQ=60)
     AGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACT                                                                                          >  SRR4302536.6254363/1‑94 (MQ=60)
      GGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTT                                                                                         <  SRR4302536.2988276/94‑1 (MQ=60)
      GGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTT                                                                                         <  SRR4302536.2481897/94‑1 (MQ=60)
         CGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTC                                                                                      <  SRR4302536.3938583/94‑1 (MQ=60)
          GGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCG                                                                                     <  SRR4302536.762911/94‑1 (MQ=60)
             ATACCATGAATATATTCGATCACTATCGTCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACAATGCAGGAGTTTCTTGCCACTTGTCGGCA                                                                                  >  SRR4302536.2054084/1‑94 (MQ=60)
             ATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCA                                                                                  >  SRR4302536.2760384/1‑94 (MQ=60)
                 CATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAT                                                                              >  SRR4302536.6211716/1‑94 (MQ=60)
                 CATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAT                                                                              <  SRR4302536.1946948/94‑1 (MQ=60)
                  ATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATC                                                                             >  SRR4302536.2333442/1‑94 (MQ=60)
                  ATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATC                                                                             >  SRR4302536.6025732/1‑94 (MQ=60)
                  ATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATC                                                                             <  SRR4302536.3598688/94‑1 (MQ=60)
                  ATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATC                                                                             >  SRR4302536.26996/1‑94 (MQ=60)
                  ATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATC                                                                             <  SRR4302536.2078261/94‑1 (MQ=60)
                    GAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGC                                                                           >  SRR4302536.5274471/1‑94 (MQ=60)
                      ATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAG                                                                         >  SRR4302536.3907967/1‑94 (MQ=60)
                        ATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTG                                                                       >  SRR4302536.6865784/1‑94 (MQ=60)
                          ATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCT                                                                     <  SRR4302536.3417943/94‑1 (MQ=60)
                          ATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCT                                                                     >  SRR4302536.5090454/1‑94 (MQ=60)
                          ATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCT                                                                     <  SRR4302536.1536565/94‑1 (MQ=60)
                          ATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCT                                                                     <  SRR4302536.5695953/94‑1 (MQ=60)
                             CGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAT                                                                  <  SRR4302536.2156626/94‑1 (MQ=60)
                             CGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAT                                                                  <  SRR4302536.4285318/94‑1 (MQ=60)
                              GATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATG                                                                 >  SRR4302536.2183579/1‑94 (MQ=60)
                              GATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATG                                                                 >  SRR4302536.2522901/1‑94 (MQ=60)
                              GATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATG                                                                 >  SRR4302536.5283478/1‑94 (MQ=60)
                               ATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGC                                                                <  SRR4302536.1318945/94‑1 (MQ=60)
                               ATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGC                                                                >  SRR4302536.6214130/1‑94 (MQ=60)
                               ATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGC                                                                >  SRR4302536.1708332/1‑94 (MQ=60)
                               ATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGC                                                                >  SRR4302536.3010420/1‑94 (MQ=60)
                                 CACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCA                                                              <  SRR4302536.3815346/94‑1 (MQ=60)
                                 CACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCA                                                              <  SRR4302536.3817227/94‑1 (MQ=60)
                                 CACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCA                                                              >  SRR4302536.4762856/1‑94 (MQ=60)
                                 CACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCA                                                              <  SRR4302536.4041760/94‑1 (MQ=60)
                                 CACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAG‑TTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAA                                                             >  SRR4302536.6218481/1‑94 (MQ=60)
                                  ACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAaactcctgcagtgtgaac                                                             <  SRR4302536.6557463/94‑19 (MQ=60)
                                  ACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAA                                                             >  SRR4302536.393206/1‑94 (MQ=60)
                                  ACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAA                                                             >  SRR4302536.1275700/1‑94 (MQ=60)
                                  ACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAA                                                             >  SRR4302536.1428341/1‑94 (MQ=60)
                                   CTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAC                                                            >  SRR4302536.6710074/1‑94 (MQ=60)
                                    TATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACG                                                           >  SRR4302536.4193054/1‑94 (MQ=60)
                                    TATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACG                                                           >  SRR4302536.1937100/1‑94 (MQ=60)
                                     ATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGC                                                          <  SRR4302536.5664134/94‑1 (MQ=60)
                                     ATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGC                                                          >  SRR4302536.2173845/1‑94 (MQ=60)
                                      TCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCG                                                         <  SRR4302536.5755381/94‑1 (MQ=60)
                                      TCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCG                                                         <  SRR4302536.698446/94‑1 (MQ=60)
                                        GCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGC                                                       >  SRR4302536.5859427/1‑94 (MQ=60)
                                        GCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGC                                                       >  SRR4302536.2424330/1‑94 (MQ=60)
                                           AGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGA                                                    <  SRR4302536.1763529/94‑1 (MQ=60)
                                           AGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGA                                                    >  SRR4302536.4971068/1‑94 (MQ=60)
                                            GCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAG                                                   <  SRR4302536.3217527/94‑1 (MQ=60)
                                            GCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAG                                                   <  SRR4302536.4219523/94‑1 (MQ=60)
                                             CGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGC                                                  >  SRR4302536.2836377/1‑94 (MQ=60)
                                              GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 >  SRR4302536.6081417/1‑94 (MQ=60)
                                              GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 <  SRR4302536.5699578/94‑1 (MQ=60)
                                               ATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGG                                                >  SRR4302536.288319/1‑94 (MQ=60)
                                               ATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGG                                                >  SRR4302536.5701391/1‑94 (MQ=60)
                                                  TGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTA                                             <  SRR4302536.606018/94‑1 (MQ=60)
                                                    AAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATT                                           <  SRR4302536.2887063/94‑1 (MQ=60)
                                                     AGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTG                                          <  SRR4302536.6608042/94‑1 (MQ=60)
                                                      GCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGA                                         <  SRR4302536.6282664/94‑1 (MQ=60)
                                                        TGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATG                                       <  SRR4302536.474207/94‑1 (MQ=60)
                                                         GCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGG                                      >  SRR4302536.1132590/1‑94 (MQ=60)
                                                            AAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTA                                   <  SRR4302536.6231108/94‑1 (MQ=60)
                                                            AAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTA                                   <  SRR4302536.3598432/94‑1 (MQ=60)
                                                            AAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTA                                   >  SRR4302536.6064804/1‑94 (MQ=60)
                                                             AGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTAT                                  <  SRR4302536.1849870/94‑1 (MQ=60)
                                                             AGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTAT                                  <  SRR4302536.705480/94‑1 (MQ=60)
                                                               GACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCG                                <  SRR4302536.4025049/94‑1 (MQ=60)
                                                               GACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCG                                <  SRR4302536.5601728/94‑1 (MQ=60)
                                                                 CGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGT                              >  SRR4302536.4957733/1‑94 (MQ=60)
                                                                    AGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAG                           <  SRR4302536.3030582/94‑1 (MQ=60)
                                                                      AGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCC                         >  SRR4302536.5991552/1‑94 (MQ=60)
                                                                        TTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTG                       <  SRR4302536.2542548/94‑1 (MQ=60)
                                                                        TTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTG                       >  SRR4302536.4022211/1‑94 (MQ=60)
                                                                         TCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGT                      <  SRR4302536.5795951/94‑1 (MQ=60)
                                                                           ACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCA                    >  SRR4302536.1738939/1‑94 (MQ=60)
                                                                            CACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCAT                   <  SRR4302536.1864184/94‑1 (MQ=60)
                                                                             ACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATG                  >  SRR4302536.1433148/1‑94 (MQ=60)
                                                                             ACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATG                  >  SRR4302536.2917913/1‑94 (MQ=60)
                                                                             ACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATG                  >  SRR4302536.3915972/1‑94 (MQ=60)
                                                                             ACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATG                  <  SRR4302536.3537707/94‑1 (MQ=60)
                                                                             ACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATG                  <  SRR4302536.2821359/94‑1 (MQ=60)
                                                                              CTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGG                 <  SRR4302536.1956629/94‑1 (MQ=60)
                                                                               TGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGT                >  SRR4302536.2138811/1‑94 (MQ=60)
                                                                               TGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGT                <  SRR4302536.2113086/94‑1 (MQ=60)
                                                                                 CAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCG              <  SRR4302536.5537081/94‑1 (MQ=60)
                                                                                 CAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCG              >  SRR4302536.6052346/1‑94 (MQ=60)
                                                                                  AGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGA             >  SRR4302536.1822202/1‑94 (MQ=60)
                                                                                  AGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGA             >  SRR4302536.4645873/1‑94 (MQ=60)
                                                                                  AGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGA             >  SRR4302536.6342088/1‑94 (MQ=60)
                                                                                   GGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGAT            <  SRR4302536.859798/94‑1 (MQ=60)
                                                                                   GGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGAT            <  SRR4302536.5750053/94‑1 (MQ=60)
                                                                                     AGTTTCTTGCCTCTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATAC          <  SRR4302536.1182383/94‑1 (MQ=60)
                                                                                     AGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATAC          >  SRR4302536.5564256/1‑94 (MQ=60)
                                                                                      GTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACA         <  SRR4302536.1174907/94‑1 (MQ=60)
                                                                                       TTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAG        <  SRR4302536.4190102/94‑1 (MQ=60)
                                                                                       TTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAG        <  SRR4302536.3737436/94‑1 (MQ=60)
                                                                                       TTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAG        <  SRR4302536.2555265/94‑1 (MQ=60)
                                                                                       TTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGAAACAG        <  SRR4302536.4439960/94‑1 (MQ=60)
                                                                                        TTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGC       >  SRR4302536.46942/1‑94 (MQ=60)
                                                                                        TTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGC       >  SRR4302536.1474209/1‑94 (MQ=60)
                                                                                         TCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCC      <  SRR4302536.859748/94‑1 (MQ=60)
                                                                                          CTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCC     >  SRR4302536.2322307/1‑94 (MQ=60)
                                                                                           TTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCA    >  SRR4302536.1588864/1‑94 (MQ=60)
                                                                                             TCCACTTGTCGGCAAGGTCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTAGTGGTGGGCCTGTCATCGTCGATACGGCCCAGG  <  SRR4302536.757020/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGG  >  NC_000913/1864914‑1865100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑