Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,973,518	A→T	D254E (GAT→GAA)	motB ←	MotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,973,518	0	A	T	100.0%	735.9 / NA	203	D254E (GAT→GAA)	motB	MotB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (93/110); total (93/110)

Only 100 of 229 total aligned reads displayed.

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG  >  NC_000913/1973428‑1973607

 ggCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttca                                                                                        <  1:793336/93‑1 (MQ=255)

 ggCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttca                                                                                        <  1:6732661/93‑1 (MQ=255)

  gCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttcat                                                                                       <  1:6157476/93‑1 (MQ=255)

  gCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttcat                                                                                       >  1:215801/1‑93 (MQ=255)

   cTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCttcatc                                                                                      <  1:5368082/93‑1 (MQ=255)

    tCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTtcatca                                                                                     >  2:475128/1‑93 (MQ=255)

     cATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAg                                                                                    <  1:4985080/93‑1 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGGTTCATCAggc                                                                                  >  2:2777554/1‑92 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAggc                                                                                  >  1:1087996/1‑92 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                  >  2:1442742/1‑93 (MQ=255)

        tCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTc                                                                                 <  2:779586/93‑1 (MQ=255)

         cTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTcc                                                                                >  1:2200955/1‑93 (MQ=255)

          tGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTTcg                                                                               >  1:5664068/1‑93 (MQ=255)

            gCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcgcg                                                                             <  2:3904814/93‑1 (MQ=255)

             cTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGa                                                                            <  1:1377608/93‑1 (MQ=255)

               ttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATc                                                                          <  2:1670723/93‑1 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCACGGCCGCCATCGCt                                                                       >  2:5644818/1‑93 (MQ=255)

                   gCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCtt                                                                      >  2:2061551/1‑93 (MQ=255)

                     gTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTaa                                                                    <  2:2465985/93‑1 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                   >  2:5074838/1‑93 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                   <  1:5173655/93‑1 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                   <  2:3349829/93‑1 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                   >  2:3556546/1‑93 (MQ=255)

                       tttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgc                                                                  <  1:6439664/93‑1 (MQ=255)

                        ttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgcg                                                                 <  1:1770332/93‑1 (MQ=255)

                         tCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgcgc                                                                >  2:4586126/1‑93 (MQ=255)

                          cATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCa                                                               <  2:1395639/93‑1 (MQ=255)

                           aTGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAt                                                              <  2:4252155/93‑1 (MQ=255)

                           aTGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAt                                                              <  1:5074885/93‑1 (MQ=255)

                            tGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATc                                                             <  2:2134561/93‑1 (MQ=255)

                              cacaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACTGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGt                                                           <  2:2907151/90‑1 (MQ=255)

                              cAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGt                                                           <  1:4171883/93‑1 (MQ=255)

                              cAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGt                                                           <  1:298360/93‑1 (MQ=255)

                              cAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGt                                                           <  1:5034080/93‑1 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          <  2:5283258/93‑1 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          >  2:6711338/1‑93 (MQ=255)

                                aaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTg                                                         <  2:2709545/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        <  1:4225649/93‑1 (MQ=255)

                                  aTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgcc                                                       <  2:411830/93‑1 (MQ=255)

                                  aTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgcc                                                       <  1:5581796/93‑1 (MQ=255)

                                  aTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgcc                                                       >  2:5943649/1‑93 (MQ=255)

                                     gCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgccgcc                                                    >  2:2599953/1‑93 (MQ=255)

                                      ccTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCa                                                   <  1:1035903/93‑1 (MQ=255)

                                       cTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAt                                                  <  1:3489603/93‑1 (MQ=255)

                                         gTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATTGCTTAAACGCATCGTTGCCGCCATGc                                                >  1:3455955/1‑93 (MQ=255)

                                         gTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGc                                                <  1:1695844/93‑1 (MQ=255)

                                          ttCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGcc                                               >  2:1872529/1‑93 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgc                                             >  1:5901432/1‑92 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcga                                             <  2:3629377/93‑1 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcga                                             <  1:3247069/93‑1 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCCTAAGCGCATCGTTGCCGCCATGCcga                                             >  2:4564889/1‑93 (MQ=255)

                                             ggCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgac                                            <  1:4259160/93‑1 (MQ=255)

                                             ggCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgac                                            >  1:4528293/1‑93 (MQ=255)

                                              gCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgacg                                           <  1:1499857/93‑1 (MQ=255)

                                                ttgtttgtTCAGTACCAGCAGGCTTATGCGACGGTTGAACGCTTCCTCAGGTCCGCGATCGCTTAAGCCCATCGTTGCCGCCATGCccacgcc                                         >  1:582537/1‑91 (MQ=255)

                                                ttgtttgtTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCgacgac                                         <  2:4517926/93‑1 (MQ=255)

                                                ttgtttgtTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCgacgac                                         >  2:295774/1‑93 (MQ=255)

                                                ttgtttgtTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCgacgac                                         >  2:2037203/1‑93 (MQ=255)

                                                ttgtttgtTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCgacgac                                         >  2:2078333/1‑93 (MQ=255)

                                                    ttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGt                                     >  1:3065830/1‑93 (MQ=255)

                                                    ttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGt                                     <  1:5239156/93‑1 (MQ=255)

                                                      gttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTaa                                   >  1:4346756/1‑93 (MQ=255)

                                                       ttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAac                                  >  1:5475232/1‑93 (MQ=255)

                                                        tCAGTACCAGCAGGCTGAGGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAaca                                 <  1:1904008/93‑1 (MQ=255)

                                                         cAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAacac                                >  1:714346/1‑93 (MQ=255)

                                                         cAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCACGATCGCTTAAGCGCATCGTTGCCGCCATGCGCACGAGACGTAacac                                >  1:665401/1‑93 (MQ=255)

                                                           gTACCAGCAGGCTGATGCGACGGTTTACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCAGACGACATGTAACACtt                              >  2:1206077/1‑93 (MQ=255)

                                                            tACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACttt                             >  2:4851446/1‑93 (MQ=255)

                                                                 gcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                        <  2:4192843/93‑1 (MQ=255)

                                                                 gcagGCTGATGAGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                        >  2:6126779/1‑93 (MQ=255)

                                                                 gcagGCTGAGGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                        <  2:4678054/93‑1 (MQ=255)

                                                                  cagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCt                       <  1:4849465/93‑1 (MQ=255)

                                                                   agGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTa                      >  2:6016221/1‑93 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAt                     >  1:5981250/1‑93 (MQ=255)

                                                                      cTGATGGGGCGGTTTACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATcc                   <  2:5239810/93‑1 (MQ=255)

                                                                      cTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATcc                   >  1:4793932/1‑93 (MQ=255)

                                                                      cTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATcc                   <  1:2874129/93‑1 (MQ=255)

                                                                       tGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCa                  >  2:5792308/1‑93 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 >  1:907759/1‑93 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 >  2:2747752/1‑93 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 >  1:2027811/1‑93 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 >  2:2231137/1‑93 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 <  2:2399552/93‑1 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 >  2:5588260/1‑93 (MQ=255)

                                                                           gCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAccc              <  2:3307467/93‑1 (MQ=255)

                                                                           gCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAccc              <  1:1926465/93‑1 (MQ=255)

                                                                           gCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAccc              <  2:5172184/93‑1 (MQ=255)

                                                                            cGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCt             <  2:3968073/93‑1 (MQ=255)

                                                                             gACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTc            <  1:427572/93‑1 (MQ=255)

                                                                              aCGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTcc           <  2:4348654/93‑1 (MQ=255)

                                                                                 gTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAc        <  1:2807705/93‑1 (MQ=255)

                                                                                  ttGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAcc       <  1:5336800/93‑1 (MQ=255)

                                                                                  ttGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGGTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGGcc       >  1:4005540/1‑93 (MQ=255)

                                                                                  gtGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAcc       <  2:2536406/92‑1 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc    <  2:1038564/93‑1 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc    <  1:939261/93‑1 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc    <  2:6569026/93‑1 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc    <  2:3083590/93‑1 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc    <  2:73467/93‑1 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc    <  2:3740398/93‑1 (MQ=255)

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG  >  NC_000913/1973428‑1973607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  NC_000913/1973426‑1973611

ACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTT                                                                                              <  SRR4302536.1160109/94‑1 (MQ=60)

ACTGGCTCATGCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTT                                                                                              <  SRR4302536.898307/94‑1 (MQ=60)

 CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             <  SRR4302536.2363163/94‑1 (MQ=60)

 CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             <  SRR4302536.1724473/94‑1 (MQ=60)

  TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCA                                                                                            >  SRR4302536.1226726/1‑94 (MQ=60)

  TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCA                                                                                            <  SRR4302536.6698796/94‑1 (MQ=60)

   GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           >  SRR4302536.221525/1‑94 (MQ=60)

   GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           <  SRR4302536.816585/94‑1 (MQ=60)

   GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           >  SRR4302536.5748148/1‑94 (MQ=60)

   GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           <  SRR4302536.6932864/94‑1 (MQ=60)

    GCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATC                                                                                          <  SRR4302536.6156348/94‑1 (MQ=60)

    GCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATC                                                                                          <  SRR4302536.6340904/94‑1 (MQ=60)

     CTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCA                                                                                         <  SRR4302536.5528156/94‑1 (MQ=60)

     CTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCA                                                                                         >  SRR4302536.5371828/1‑94 (MQ=60)

      TCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAG                                                                                        <  SRR4302536.3133556/94‑1 (MQ=60)

      TCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAG                                                                                        >  SRR4302536.2986055/1‑94 (MQ=60)

      TCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAG                                                                                        >  SRR4302536.824113/1‑94 (MQ=60)

       CATTCTGGCTTTGGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCCGGCTGATGCGACGGTTGACGGATTCATCAGG                                                                                       <  SRR4302536.5143652/94‑1 (MQ=60)

       CATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGG                                                                                       <  SRR4302536.5134767/94‑1 (MQ=60)

        ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      >  SRR4302536.712251/1‑94 (MQ=60)

        ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGC                                                                                      >  SRR4302536.1120621/1‑94 (MQ=60)

         TTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTC                                                                                     <  SRR4302536.6015624/94‑1 (MQ=60)

          TCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCC                                                                                    <  SRR4302536.2668171/94‑1 (MQ=60)

          TCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCC                                                                                    >  SRR4302536.2267397/1‑94 (MQ=60)

          TCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCC                                                                                    <  SRR4302536.3395771/94‑1 (MQ=60)

           CTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTTCG                                                                                   >  SRR4302536.5832418/1‑94 (MQ=60)

             GGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCG                                                                                 <  SRR4302536.4148059/94‑1 (MQ=60)

               CTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAT                                                                               <  SRR4302536.1418641/94‑1 (MQ=60)

                  TCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGC                                                                            >  SRR4302536.1993579/1‑94 (MQ=60)

                    GGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTT                                                                          >  SRR4302536.460989/1‑94 (MQ=60)

                    GGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGGTCGCGATCGCTT                                                                          >  SRR4302536.1764003/1‑94 (MQ=60)

                     GCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTA                                                                         <  SRR4302536.3949739/94‑1 (MQ=60)

                      CGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAAGTCCGCGATCGCCTAA                                                                        >  SRR4302536.618376/1‑94 (MQ=60)

                       GTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAG                                                                       <  SRR4302536.942753/94‑1 (MQ=60)

                        TTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGC                                                                      <  SRR4302536.5328011/94‑1 (MQ=60)

                         TTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCG                                                                     >  SRR4302536.4410626/1‑94 (MQ=60)

                         TTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCG                                                                     <  SRR4302536.6632090/94‑1 (MQ=60)

                         TTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTGGACGGCTTCAGCAGGTCCGCGATCGCTTAAGCG                                                                     <  SRR4302536.3259318/94‑1 (MQ=60)

                          TTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGC                                                                    <  SRR4302536.1823904/94‑1 (MQ=60)

                            CATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAT                                                                  <  SRR4302536.5235341/94‑1 (MQ=60)

                             ATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATC                                                                 <  SRR4302536.160570/94‑1 (MQ=60)

                             ATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATC                                                                 <  SRR4302536.5226802/94‑1 (MQ=60)

                              TGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCA                                                                <  SRR4302536.519328/94‑1 (MQ=60)

                                CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              <  SRR4302536.4295820/94‑1 (MQ=60)

                                CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              <  SRR4302536.5952049/94‑1 (MQ=60)

                                CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              <  SRR4302536.306285/94‑1 (MQ=60)

                                CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              <  SRR4302536.5184970/94‑1 (MQ=60)

                                   AATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCC                                                           <  SRR4302536.4351382/94‑1 (MQ=60)

                                    ATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCG                                                          >  SRR4302536.3577526/1‑94 (MQ=60)

                                    ATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCG                                                          <  SRR4302536.5747900/94‑1 (MQ=60)

                                        CCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAT                                                      <  SRR4302536.1066641/94‑1 (MQ=60)

                                        CCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAT                                                      <  SRR4302536.2247605/94‑1 (MQ=60)

                                         CTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATG                                                     <  SRR4302536.3593688/94‑1 (MQ=60)

                                          TGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATTGCTTAAACGCATCGTTGCCGCCATGC                                                    >  SRR4302536.3558894/1‑94 (MQ=60)

                                           GTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCC                                                   <  SRR4302536.1744933/94‑1 (MQ=60)

                                             TCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGC                                                 >  SRR4302536.6077180/1‑94 (MQ=60)

                                             TCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGA                                                 <  SRR4302536.1073208/94‑1 (MQ=60)

                                              tgtgTTGTTTGTTCAGTACCCGCCGGGTGATGGGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                <  SRR4302536.1721383/90‑1 (MQ=60)

                                              CGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                >  SRR4302536.4664051/1‑94 (MQ=60)

                                              CGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                <  SRR4302536.3342275/94‑1 (MQ=60)

                                              CGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                <  SRR4302536.196997/94‑1 (MQ=60)

                                               GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               <  SRR4302536.471905/94‑1 (MQ=60)

                                               GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               <  SRR4302536.4386074/94‑1 (MQ=60)

                                               GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               >  SRR4302536.4945804/1‑94 (MQ=60)

                                               GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               >  SRR4302536.5114981/1‑94 (MQ=60)

                                               GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               >  SRR4302536.5492566/1‑94 (MQ=60)

                                                GCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGA                                              <  SRR4302536.1544110/94‑1 (MQ=60)

                                                 CTTGTTTGTTCAGTACCAGCAGGCTTATGCGACGGTTGAACGCTTCCTCAGGTCCGCGATCGCTTAAGCCCATCGTTGCCGCCATGCCCACGCC                                             >  SRR4302536.597349/1‑94 (MQ=60)

                                                 CTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGAC                                             <  SRR4302536.792377/94‑1 (MQ=60)

                                                    TTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACG                                          <  SRR4302536.5684534/94‑1 (MQ=60)

                                                     TTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGT                                         >  SRR4302536.3155718/1‑94 (MQ=60)

                                                      TTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTA                                        <  SRR4302536.2096563/94‑1 (MQ=60)

                                                      TTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTA                                        <  SRR4302536.5395257/94‑1 (MQ=60)

                                                       TGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAA                                       >  SRR4302536.4476862/1‑94 (MQ=60)

                                                       TGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAA                                       >  SRR4302536.144370/1‑94 (MQ=60)

                                                        GTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAAC                                      >  SRR4302536.5638776/1‑94 (MQ=60)

                                                         TTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACA                                     <  SRR4302536.3127929/94‑1 (MQ=60)

                                                          TCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACAC                                    >  SRR4302536.735232/1‑94 (MQ=60)

                                                          TCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCACGATCGCTTAAGCGCATCGTTGCCGCCATGCGCACGAGACGTAACAC                                    >  SRR4302536.684918/1‑94 (MQ=60)

                                                          TCAGTACCAGCAGGCTGAGGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACAC                                    <  SRR4302536.1961335/94‑1 (MQ=60)

                                                            AGTACCAGCAGGCTGATGCTACGTTTAACGGCTTCATCAGGTCCGGGATCGCTTAAACACATCGTTGCCGCCATGCCGACGACACGTAACACTT                                  <  SRR4302536.5709172/94‑1 (MQ=60)

                                                            AGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTT                                  <  SRR4302536.5173485/94‑1 (MQ=60)

                                                            AGTACCAGCAGGCTGATGCGACGGTTGACAGCTTCATCAGGTCCGCGATCGCTTAAGCGCCTCGTTGCCGCCATGACGACGACACGTAACACTT                                  >  SRR4302536.4851198/1‑94 (MQ=60)

                                                             GTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTT                                 <  SRR4302536.4381357/94‑1 (MQ=60)

                                                                 CAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGACG                             >  SRR4302536.1879735/1‑94 (MQ=60)

                                                                   GCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCT                           <  SRR4302536.4906816/94‑1 (MQ=60)

                                                                   GCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCT                           <  SRR4302536.770402/94‑1 (MQ=60)

                                                                    CAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTA                          <  SRR4302536.4995385/94‑1 (MQ=60)

                                                                     AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         >  SRR4302536.5543365/1‑94 (MQ=60)

                                                                     AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         >  SRR4302536.6159588/1‑94 (MQ=60)

                                                                      GGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATC                        >  SRR4302536.6775913/1‑94 (MQ=60)

                                                                      GGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATC                        >  SRR4302536.4311878/1‑94 (MQ=60)

                                                                       GCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCC                       >  SRR4302536.4938229/1‑94 (MQ=60)

                                                                       GCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCC                       <  SRR4302536.2936306/94‑1 (MQ=60)

                                                                        CTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCA                      >  SRR4302536.5743221/1‑94 (MQ=60)

                                                                        CTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCA                      <  SRR4302536.2958988/94‑1 (MQ=60)

                                                                         TGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAA                     >  SRR4302536.2088864/1‑94 (MQ=60)

                                                                         TGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAA                     >  SRR4302536.6187319/1‑94 (MQ=60)

                                                                         TGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAA                     >  SRR4302536.934501/1‑94 (MQ=60)

                                                                            TGCGACGTTTGACGGTTTCATCAGGTCCGCGATTGCTTATTCGCATCGTTGCCGCCATGCCGACGACACGTAACTCTTTGCCGCTATCCAACCT                  >  SRR4302536.6927510/1‑94 (MQ=60)

                                                                             GCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCT                 <  SRR4302536.1984426/94‑1 (MQ=60)

                                                                               GACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCC               <  SRR4302536.438828/94‑1 (MQ=60)

                                                                               GACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCC               <  SRR4302536.1968756/94‑1 (MQ=60)

                                                                                ACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGGCGCTATCCAACCCTCCG              >  SRR4302536.5397284/1‑94 (MQ=60)

                                                                                ACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCG              <  SRR4302536.4118221/94‑1 (MQ=60)

                                                                                  GGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAC            >  SRR4302536.5509160/1‑94 (MQ=60)

                                                                                   GTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACC           <  SRR4302536.2890956/94‑1 (MQ=60)

                                                                                   GTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGGTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGGCC           >  SRR4302536.4124399/1‑94 (MQ=60)

                                                                                    TTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCA          <  SRR4302536.5496252/94‑1 (MQ=60)

                                                                                      GACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATC        >  SRR4302536.60631/1‑94 (MQ=60)

                                                                                       GCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCA       <  SRR4302536.4353033/94‑1 (MQ=60)

                                                                                       ACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCA       <  SRR4302536.6500790/94‑1 (MQ=60)

                                                                                       ACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCA       <  SRR4302536.966960/94‑1 (MQ=60)

                                                                                       ACGGCTTCATCACGTCCGCGATCGCTTAAGCCCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCA       <  SRR4302536.720533/94‑1 (MQ=60)

                                                                                        CGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG      <  SRR4302536.4396146/94‑1 (MQ=60)

                                                                                         GGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGT     <  SRR4302536.241853/94‑1 (MQ=60)

                                                                                         GGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGT     >  SRR4302536.2013412/1‑94 (MQ=60)

                                                                                          GCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTT    <  SRR4302536.6307404/94‑1 (MQ=60)

                                                                                           CTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTC   <  SRR4302536.6049397/94‑1 (MQ=60)

                                                                                           CTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTC   <  SRR4302536.6049396/94‑1 (MQ=60)

                                                                                            TTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  <  SRR4302536.5828316/94‑1 (MQ=60)

ACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  NC_000913/1973426‑1973611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`