Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,025,930	G→A	S42F (TCC→TTC)	b1956 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,025,930	0	G	A	100.0%	842.3 / NA	220	S42F (TCC→TTC)	b1956	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (98/122); total (98/122)

Only 100 of 234 total aligned reads displayed.

AGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGGAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCC  >  NC_000913/2025840‑2026019

aGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGaaa                                                                                         >  2:1836172/1‑93 (MQ=255)

aGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGaaa                                                                                         >  1:3476766/1‑93 (MQ=255)

   gcgTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGaaaaaa                                                                                      >  1:6325088/1‑93 (MQ=255)

   gcgTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGaaaaaa                                                                                      <  1:6249145/93‑1 (MQ=255)

     gTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAg                                                                                    >  2:1331534/1‑93 (MQ=255)

      ttGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGc                                                                                   <  2:3339181/93‑1 (MQ=255)

      tgggCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGc                                                                                   >  2:6481720/3‑93 (MQ=255)

         gCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAAt                                                                                <  2:1082346/93‑1 (MQ=255)

         gCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAAt                                                                                <  1:3353251/93‑1 (MQ=255)

           aaCGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATAc                                                                              >  2:6154906/1‑93 (MQ=255)

           aaCGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATAc                                                                              >  2:6326456/1‑93 (MQ=255)

           aaCGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATAc                                                                              <  2:4379279/93‑1 (MQ=255)

            aCGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATAcc                                                                             >  2:3026823/1‑93 (MQ=255)

             cGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCa                                                                            >  1:6095233/1‑93 (MQ=255)

              gTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCAc                                                                           >  1:98203/1‑93 (MQ=255)

               ttttCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACg                                                                          <  1:2957266/93‑1 (MQ=255)

                tttCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGa                                                                         <  1:2329172/93‑1 (MQ=255)

                tttCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGa                                                                         >  1:1288718/1‑93 (MQ=255)

                tttCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGa                                                                         >  2:4283857/1‑93 (MQ=255)

                 ttCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGAt                                                                        <  1:2546695/93‑1 (MQ=255)

                    cAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATaaa                                                                     <  1:4041/93‑1 (MQ=255)

                    cAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACAACGATaaa                                                                     >  1:5761808/1‑93 (MQ=255)

                     aGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAg                                                                    <  2:2074271/93‑1 (MQ=255)

                     aGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAg                                                                    <  1:553323/93‑1 (MQ=255)

                       atgatTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCa                                                                  <  1:2102689/93‑1 (MQ=255)

                       atgatTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCa                                                                  <  1:785076/93‑1 (MQ=255)

                       atgatTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCa                                                                  <  2:3828935/93‑1 (MQ=255)

                        tgatTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCag                                                                 <  1:6504569/93‑1 (MQ=255)

                         gatTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCaga                                                                <  2:140835/93‑1 (MQ=255)

                         gatTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCaga                                                                <  1:2183927/93‑1 (MQ=255)

                         gatTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCaga                                                                <  2:5335633/93‑1 (MQ=255)

                          atTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCagag                                                               >  2:3908898/1‑93 (MQ=255)

                            tACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGAt                                                             <  2:4996448/93‑1 (MQ=255)

                             aCGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGAtt                                                            <  1:1051529/93‑1 (MQ=255)

                              cGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTa                                                           <  1:440009/93‑1 (MQ=255)

                               gctggctgGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTaa                                                          >  1:5236179/1‑93 (MQ=255)

                                ctggctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAAc                                                         <  1:2122189/93‑1 (MQ=255)

                                ctggctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAAc                                                         <  1:676061/93‑1 (MQ=255)

                                ctggctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGCTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAAc                                                         <  2:6647083/93‑1 (MQ=255)

                                 tggctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACg                                                        >  1:1621158/1‑93 (MQ=255)

                                 tggctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACg                                                        >  1:4759665/1‑93 (MQ=255)

                                 tggctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACg                                                        >  2:617494/1‑93 (MQ=255)

                                  ggctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGa                                                       <  1:6409030/93‑1 (MQ=255)

                                    ctggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAacaaca                                                     <  1:3624247/93‑1 (MQ=255)

                                      ggAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATg                                                   <  1:2608924/93‑1 (MQ=255)

                                       gagaTATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGa                                                  <  2:692619/93‑1 (MQ=255)

                                        agaTATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGAc                                                 <  2:5764215/93‑1 (MQ=255)

                                         gaTATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGAcc                                                >  2:2944424/1‑93 (MQ=255)

                                         gaTATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGAcc                                                <  2:2239872/93‑1 (MQ=255)

                                          atatAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCa                                               <  1:5910987/93‑1 (MQ=255)

                                          atatAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCa                                               <  2:805550/93‑1 (MQ=255)

                                           tataGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAg                                              >  1:3280193/1‑93 (MQ=255)

                                            ataGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAgc                                             >  2:3365411/1‑92 (MQ=255)

                                             taGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGc                                            >  2:3700516/1‑93 (MQ=255)

                                              aGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGcc                                           >  2:5103914/1‑93 (MQ=255)

                                               ggCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGccc                                          <  1:6051108/93‑1 (MQ=255)

                                               ggCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGccc                                          >  1:5091504/1‑93 (MQ=255)

                                                gCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGcccc                                         >  1:833481/1‑93 (MQ=255)

                                                gCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGcccc                                         <  1:5342368/93‑1 (MQ=255)

                                                 cATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCa                                        <  2:4681723/93‑1 (MQ=255)

                                                 cATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCa                                        <  2:2464557/93‑1 (MQ=255)

                                                  aTCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAg                                       >  1:1954043/1‑93 (MQ=255)

                                                  aTCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAg                                       >  2:6271476/1‑93 (MQ=255)

                                                   tCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAgg                                      >  2:1769616/1‑93 (MQ=255)

                                                     ttCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAggcg                                    >  2:2199051/1‑93 (MQ=255)

                                                     ttCCAGCACCACCACCTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAggcg                                    >  1:2918178/1‑93 (MQ=255)

                                                      tCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAggcgg                                   <  2:4719943/93‑1 (MQ=255)

                                                       ccAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAggcggc                                  >  1:954351/1‑93 (MQ=255)

                                                         aGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGt                                <  2:6683476/93‑1 (MQ=255)

                                                          gCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTg                               >  1:3091582/1‑93 (MQ=255)

                                                           caccaccacTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGc                              <  1:5322249/93‑1 (MQ=255)

                                                            accaccacTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCg                             >  2:6501614/1‑93 (MQ=255)

                                                              caccacTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAg                           <  2:4219315/93‑1 (MQ=255)

                                                                ccacTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGtt                         <  2:1365453/93‑1 (MQ=255)

                                                                 cacTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGttt                        <  1:3671554/93‑1 (MQ=255)

                                                                  acTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGtttt                       >  1:2816279/1‑93 (MQ=255)

                                                                  acTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGtttt                       >  1:3905108/1‑93 (MQ=255)

                                                                   ctccACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCGCGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGttttt                      <  2:2431150/90‑1 (MQ=255)

                                                                    ttCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGtttttt                     >  2:3201424/1‑93 (MQ=255)

                                                                    ttCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGtttttt                     <  2:3145994/93‑1 (MQ=255)

                                                                    ttCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGtttttt                     >  2:1973217/1‑93 (MQ=255)

                                                                     tCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTc                    <  2:1492453/93‑1 (MQ=255)

                                                                     tCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTc                    >  1:1541979/1‑93 (MQ=255)

                                                                       aCGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCaa                  <  1:1824018/93‑1 (MQ=255)

                                                                        cGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAAc                 <  2:1727965/93‑1 (MQ=255)

                                                                         gCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAAcc                <  1:5855357/93‑1 (MQ=255)

                                                                          ccAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCa               >  2:838721/1‑93 (MQ=255)

                                                                          ccAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCa               <  1:1438243/93‑1 (MQ=255)

                                                                           cAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAg              >  2:2607667/1‑93 (MQ=255)

                                                                           cAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAg              >  1:4230114/1‑93 (MQ=255)

                                                                            aGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACAAGGCCCCAGGCGGCGTGCGAGTTTTGTCAACCAGc             >  2:584855/1‑93 (MQ=255)

                                                                             ggTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGct            >  2:1196404/1‑93 (MQ=255)

                                                                             ggTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGct            >  1:5599295/1‑93 (MQ=255)

                                                                               tGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGctct          >  1:2082961/1‑93 (MQ=255)

                                                                                gAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTg         <  2:5178021/93‑1 (MQ=255)

                                                                                 aGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTgg        >  2:551089/1‑93 (MQ=255)

                                                                                   cAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGtt      <  2:3512710/93‑1 (MQ=255)

                                                                                    aaGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGttt     >  2:2424469/1‑93 (MQ=255)

                                                                                    aaGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGttt     >  1:1161451/1‑93 (MQ=255)

                                                                                       gTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTcc  <  1:5496213/93‑1 (MQ=255)

AGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGGAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCC  >  NC_000913/2025840‑2026019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGGAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATT  >  NC_000913/2025839‑2026022

GAGCGCGTTGGCAACGTTTTCCAGATGCTTACGCTTGCTGGAGATATATGCATCTTCCAGCACCACCACTTTACGCCAGGGGAGCAAAGTGAAA                                                                                            >  SRR4302536.6175008/1‑94 (MQ=60)

GAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAA                                                                                            >  SRR4302536.3580398/1‑94 (MQ=60)

 AGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAA                                                                                           >  SRR4302536.2314921/1‑94 (MQ=60)

  GCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAA                                                                                          <  SRR4302536.6243029/94‑1 (MQ=60)

   CGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAA                                                                                         >  SRR4302536.6513482/1‑94 (MQ=60)

   CGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGACATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAA                                                                                         <  SRR4302536.5543986/94‑1 (MQ=60)

    GCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAA                                                                                        <  SRR4302536.6435315/94‑1 (MQ=60)

     CGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAG                                                                                       >  SRR4302536.5687702/1‑94 (MQ=60)

     CGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACCCCAGGTGAGCAAGGTGAAAAAAAG                                                                                       <  SRR4302536.816431/94‑1 (MQ=60)

      GTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGC                                                                                      <  SRR4302536.2671257/94‑1 (MQ=60)

       TTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCA                                                                                     <  SRR4302536.3514332/94‑1 (MQ=60)

         GGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAAT                                                                                   <  SRR4302536.4357930/94‑1 (MQ=60)

          GCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATA                                                                                  <  SRR4302536.3452747/94‑1 (MQ=60)

          GCAACGTTTTCCAGATGATTACGCTAGCTGGCGATATAGGCATCTTCCAGCACCACCACTTCACTCCAGGTAAGCAAGGTGAAAAAAAGaaatc                                                                                  >  SRR4302536.1370467/1‑89 (MQ=60)

           CAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATAC                                                                                 <  SRR4302536.1146971/94‑1 (MQ=60)

           CAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATAC                                                                                 <  SRR4302536.6770613/94‑1 (MQ=60)

           CAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATAC                                                                                 >  SRR4302536.8877/1‑94 (MQ=60)

            AACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACC                                                                                >  SRR4302536.3967413/1‑94 (MQ=60)

             ACGTTTTCCAGATGATTACGCTGGCTGGATATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCA                                                                               <  SRR4302536.2242250/94‑1 (MQ=60)

             ACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCA                                                                               >  SRR4302536.3499329/1‑94 (MQ=60)

             ACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCA                                                                               >  SRR4302536.6276975/1‑94 (MQ=60)

              CGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCAC                                                                              >  SRR4302536.100819/1‑94 (MQ=60)

               GTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACG                                                                             <  SRR4302536.6072485/94‑1 (MQ=60)

               GTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACG                                                                             <  SRR4302536.2293284/94‑1 (MQ=60)

                TTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGA                                                                            >  SRR4302536.1327273/1‑94 (MQ=60)

                TTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGA                                                                            <  SRR4302536.3044394/94‑1 (MQ=60)

                 TTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGAT                                                                           <  SRR4302536.6494168/94‑1 (MQ=60)

                 TTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGAT                                                                           <  SRR4302536.2155570/94‑1 (MQ=60)

                 TTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGAT                                                                           <  SRR4302536.3095026/94‑1 (MQ=60)

                 TTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGAT                                                                           <  SRR4302536.2399854/94‑1 (MQ=60)

                  TTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATA                                                                          <  SRR4302536.2623399/94‑1 (MQ=60)

                   TCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAA                                                                         >  SRR4302536.2962315/1‑94 (MQ=60)

                    CCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACAACGATAAA                                                                        >  SRR4302536.5933204/1‑94 (MQ=60)

                     CAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAG                                                                       <  SRR4302536.5723015/94‑1 (MQ=60)

                     CAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAG                                                                       <  SRR4302536.4144/94‑1 (MQ=60)

                     CAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAG                                                                       >  SRR4302536.2839879/1‑94 (MQ=60)

                      AGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGC                                                                      <  SRR4302536.567422/94‑1 (MQ=60)

                        ATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAG                                                                    <  SRR4302536.808066/94‑1 (MQ=60)

                        ATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAG                                                                    <  SRR4302536.506496/94‑1 (MQ=60)

                        ATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAG                                                                    <  SRR4302536.2165908/94‑1 (MQ=60)

                         TGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGA                                                                   <  SRR4302536.531409/94‑1 (MQ=60)

                         TGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGA                                                                   <  SRR4302536.6698430/94‑1 (MQ=60)

                         AGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGA                                                                   <  SRR4302536.5982753/94‑1 (MQ=60)

                          GATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAG                                                                  <  SRR4302536.2249760/94‑1 (MQ=60)

                          GATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAG                                                                  <  SRR4302536.1749522/94‑1 (MQ=60)

                          GATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAG                                                                  <  SRR4302536.2158577/94‑1 (MQ=60)

                           ATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGA                                                                 <  SRR4302536.5362622/94‑1 (MQ=60)

                             TACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATT                                                               <  SRR4302536.6837245/94‑1 (MQ=60)

                              ACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTA                                                              <  SRR4302536.1082921/94‑1 (MQ=60)

                               CGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAA                                                             <  SRR4302536.451601/94‑1 (MQ=60)

                               CGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAA                                                             >  SRR4302536.5392184/1‑94 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           >  SRR4302536.6137057/1‑94 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           >  SRR4302536.4902992/1‑94 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           <  SRR4302536.3221533/94‑1 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           <  SRR4302536.2185957/94‑1 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           >  SRR4302536.1028386/1‑94 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           >  SRR4302536.1668381/1‑94 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           >  SRR4302536.1581918/1‑94 (MQ=60)

                                 CTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACG                                                           <  SRR4302536.695842/94‑1 (MQ=60)

                                  TGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGA                                                          >  SRR4302536.3655179/1‑94 (MQ=60)

                                   GGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGAC                                                         <  SRR4302536.6600460/94‑1 (MQ=60)

                                     CTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACAT                                                       <  SRR4302536.5781488/94‑1 (MQ=60)

                                     CTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACAACAT                                                       <  SRR4302536.3732632/94‑1 (MQ=60)

                                      TGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATG                                                      <  SRR4302536.4892819/94‑1 (MQ=60)

                                      TGGAGATATAGGAATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATG                                                      <  SRR4302536.6540607/94‑1 (MQ=60)

                                       GGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGA                                                     <  SRR4302536.2687216/94‑1 (MQ=60)

                                        GAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGAC                                                    <  SRR4302536.1459296/94‑1 (MQ=60)

                                         AGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACC                                                   <  SRR4302536.3016978/94‑1 (MQ=60)

                                           ATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAG                                                 <  SRR4302536.6087048/94‑1 (MQ=60)

                                           ATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAG                                                 >  SRR4302536.3376644/1‑94 (MQ=60)

                                               AGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCC                                             >  SRR4302536.5243770/1‑94 (MQ=60)

                                                GGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCC                                            >  SRR4302536.857995/1‑94 (MQ=60)

                                                GGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCC                                            <  SRR4302536.6231589/94‑1 (MQ=60)

                                                 GCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCA                                           <  SRR4302536.6068681/94‑1 (MQ=60)

                                                 GCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCA                                           <  SRR4302536.5501924/94‑1 (MQ=60)

                                                  CATCTTCCAGCGCCACGACTTCACGCAAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAG                                          <  SRR4302536.5494583/94‑1 (MQ=60)

                                                  CATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAG                                          >  SRR4302536.2012802/1‑94 (MQ=60)

                                                  CATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAG                                          >  SRR4302536.4017180/1‑94 (MQ=60)

                                                     CTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCG                                       <  SRR4302536.2541945/94‑1 (MQ=60)

                                                     CTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCG                                       <  SRR4302536.1688532/94‑1 (MQ=60)

                                                     CTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCG                                       >  SRR4302536.430292/1‑94 (MQ=60)

                                                     CTTCCAGCACCACCACCTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCG                                       >  SRR4302536.3004313/1‑94 (MQ=60)

                                                       TCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGC                                     >  SRR4302536.982563/1‑94 (MQ=60)

                                                        CCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCG                                    >  SRR4302536.5426882/1‑94 (MQ=60)

                                                         CAGCACCACCACTTCACGCCAGGTGAGCAAGACGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGT                                   >  SRR4302536.6039025/1‑94 (MQ=60)

                                                          AGCACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTG                                  >  SRR4302536.3181956/1‑94 (MQ=60)

                                                            CACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCG                                <  SRR4302536.5481241/94‑1 (MQ=60)

                                                            CACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCG                                <  SRR4302536.2771502/94‑1 (MQ=60)

                                                            CACCACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGACCCAGGCGGCGTGCG                                >  SRR4302536.4462959/1‑94 (MQ=60)

                                                               CACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGT                             <  SRR4302536.1965561/94‑1 (MQ=60)

                                                                ACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTT                            <  SRR4302536.3172392/94‑1 (MQ=60)

                                                                ACCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTT                            >  SRR4302536.3408418/1‑94 (MQ=60)

                                                                 CCACTTCAGGCCAGGGGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTT                           <  SRR4302536.5016177/94‑1 (MQ=60)

                                                                 CCACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTT                           <  SRR4302536.1024369/94‑1 (MQ=60)

                                                                  CACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTT                          <  SRR4302536.1619314/94‑1 (MQ=60)

                                                                  CACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTT                          >  SRR4302536.2899715/1‑94 (MQ=60)

                                                                  CACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTT                          >  SRR4302536.4021172/1‑94 (MQ=60)

                                                                  CACTTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTT                          <  SRR4302536.3781329/94‑1 (MQ=60)

                                                                     TTCACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTC                       >  SRR4302536.1587307/1‑94 (MQ=60)

                                                                        ACGCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAAC                    <  SRR4302536.1879037/94‑1 (MQ=60)

                                                                          GCCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCA                  <  SRR4302536.6029805/94‑1 (MQ=60)

                                                                           CCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAG                 <  SRR4302536.1480896/94‑1 (MQ=60)

                                                                           CCAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAG                 >  SRR4302536.4355984/1‑94 (MQ=60)

                                                                            CAGGTGAGCAAGGTGAAAATGAGCAATTCCACGATAAAGCAGAGATTAACGACAGGACCAGGCCCCAGGCGGCTGGCGAGTTTTTTGAACCAGC                <  SRR4302536.4123715/94‑1 (MQ=60)

                                                                            CAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGC                >  SRR4302536.6288780/1‑94 (MQ=60)

                                                                            CAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGC                >  SRR4302536.890033/1‑94 (MQ=60)

                                                                            CAGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGC                >  SRR4302536.5591884/1‑94 (MQ=60)

                                                                             AGGTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCT               >  SRR4302536.5765726/1‑94 (MQ=60)

                                                                               GTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCT             <  SRR4302536.3754779/94‑1 (MQ=60)

                                                                               GTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCT             <  SRR4302536.6440136/94‑1 (MQ=60)

                                                                               GTGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCT             >  SRR4302536.2145656/1‑94 (MQ=60)

                                                                                TGAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTG            >  SRR4302536.710783/1‑94 (MQ=60)

                                                                                 GAGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGG           >  SRR4302536.1320726/1‑94 (MQ=60)

                                                                                  AGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACGTGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGT          <  SRR4302536.4025077/94‑1 (MQ=60)

                                                                                  AGCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGT          >  SRR4302536.99577/1‑94 (MQ=60)

                                                                                  AGCAAGGTGAAAAAAAGCAATACCACGAAAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCGGCTTTGGT          >  SRR4302536.2182780/1‑94 (MQ=60)

                                                                                   GCAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTT         <  SRR4302536.6659933/94‑1 (MQ=60)

                                                                                    CAAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTT        >  SRR4302536.1196419/1‑94 (MQ=60)

                                                                                     AAGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTT       >  SRR4302536.5785406/1‑94 (MQ=60)

                                                                                      AGGTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGCTTTC      >  SRR4302536.2060888/1‑94 (MQ=60)

                                                                                        GTGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCA    <  SRR4302536.5660221/94‑1 (MQ=60)

                                                                                         TGAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCAT   <  SRR4302536.4545582/94‑1 (MQ=60)

                                                                                          GAAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATT  >  SRR4302536.2218316/1‑94 (MQ=60)

GAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGGTGGAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATT  >  NC_000913/2025839‑2026022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`