Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,106,960	T→C	D216G (GAC→GGC)	rfbX ←	putative O‑antigen transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,960	0	T	C	99.6%	805.4 / ‑4.8	249	D216G (GAC→GGC)	rfbX	putative O‑antigen transporter
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (136/112); total (136/113)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.03e-01

Only 100 of 253 total aligned reads displayed.

GACGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCA  >  NC_000913/2106870‑2107049

gACGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCt                                                                                         <  2:1924225/93‑1 (MQ=255)

gACGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCt                                                                                         >  2:302483/1‑93 (MQ=255)

gACGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCt                                                                                         <  2:3154275/93‑1 (MQ=255)

  cGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGc                                                                                       <  2:3363171/93‑1 (MQ=255)

  cGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGc                                                                                       <  2:4350957/93‑1 (MQ=255)

   gTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAGCCGCCTGCt                                                                                      <  1:438096/93‑1 (MQ=255)

    ttGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTa                                                                                     >  2:601176/1‑93 (MQ=255)

      ggTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTaaa                                                                                   <  2:3939727/93‑1 (MQ=255)

      ggTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTaaa                                                                                   >  2:6200055/1‑93 (MQ=255)

      ggTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTaaa                                                                                   <  1:2231529/93‑1 (MQ=255)

      ggTCAAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTaaa                                                                                   <  1:3470174/93‑1 (MQ=255)

       gTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAg                                                                                  >  2:6299686/1‑93 (MQ=255)

        tCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGa                                                                                 >  2:1768294/1‑93 (MQ=255)

         ccAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGAt                                                                                <  2:2857090/93‑1 (MQ=255)

         ccAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGAt                                                                                <  1:3295548/93‑1 (MQ=255)

            gATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGa                                                                             <  1:5920256/93‑1 (MQ=255)

            gATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGa                                                                             <  2:2418846/93‑1 (MQ=255)

            gATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGa                                                                             <  1:5796861/93‑1 (MQ=255)

             atatAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGAc                                                                            >  2:1336222/1‑93 (MQ=255)

              tataAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACg                                                                           >  2:2855579/1‑93 (MQ=255)

              tataAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACg                                                                           >  1:4210718/1‑93 (MQ=255)

               ataAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGc                                                                          <  1:4152417/93‑1 (MQ=255)

                 aaaTCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGcac                                                                        >  1:4249843/1‑93 (MQ=255)

                  aaTCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGcaca                                                                       <  1:1957905/93‑1 (MQ=255)

                      ccAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTAt                                                                   >  2:5566128/1‑93 (MQ=255)

                       cAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGGACATTATg                                                                  >  1:2554661/1‑93 (MQ=255)

                        aGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGt                                                                 >  1:1014905/1‑93 (MQ=255)

                         ggATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTa                                                                >  2:488991/1‑93 (MQ=255)

                         ggATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTa                                                                >  2:4038632/1‑93 (MQ=255)

                          gATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTaa                                                               >  1:2015235/1‑93 (MQ=255)

                          gATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTaa                                                               <  1:1082599/93‑1 (MQ=255)

                             aataaCTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGa                                                            >  1:3844444/1‑93 (MQ=255)

                              ataaCTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGAt                                                           >  2:1524790/1‑93 (MQ=255)

                               taaCTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATa                                                          <  1:6635666/93‑1 (MQ=255)

                                aaCTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAg                                                         <  1:5417034/93‑1 (MQ=255)

                                 aCTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGc                                                        >  1:6380050/1‑93 (MQ=255)

                                 aCTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGATAAAGATCGACGCACATTATGTAATGATAGc                                                        <  1:3131642/93‑1 (MQ=255)

                                   tATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGt                                                      >  2:749964/1‑93 (MQ=255)

                                   tATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGt                                                      >  2:4990898/1‑93 (MQ=255)

                                    aTTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTa                                                     >  2:5733040/1‑93 (MQ=255)

                                    aTTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTa                                                     <  1:4597312/93‑1 (MQ=255)

                                      tCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAAc                                                   <  2:3711711/93‑1 (MQ=255)

                                      tCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAAc                                                   <  2:6127429/93‑1 (MQ=255)

                                        ccGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACtt                                                 <  1:744720/93‑1 (MQ=255)

                                             gAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCa                                            >  1:1527834/1‑93 (MQ=255)

                                              aaTATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCaa                                           >  1:1364895/1‑93 (MQ=255)

                                               atatAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAAt                                          >  2:2972910/1‑93 (MQ=255)

                                               atatAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAAt                                          <  2:1038162/93‑1 (MQ=255)

                                                tataAACTAATGGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATc                                         >  1:2358942/1‑93 (MQ=255)

                                                tataAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATc                                         >  1:4898607/1‑93 (MQ=255)

                                                    aaCTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAAc                                     >  1:1428441/1‑93 (MQ=255)

                                                    aaCTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAAc                                     <  1:1284216/93‑1 (MQ=255)

                                                     aCTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAAcc                                    <  1:3104084/93‑1 (MQ=255)

                                                      cTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCt                                   >  1:2899029/1‑93 (MQ=255)

                                                        aaTAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTc                                 <  2:4515032/93‑1 (MQ=255)

                                                         aTAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCa                                >  2:3417671/1‑93 (MQ=255)

                                                         aTAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCa                                <  1:6526866/93‑1 (MQ=255)

                                                         aTAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCa                                >  2:267176/1‑93 (MQ=255)

                                                           aGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATg                              >  1:1883318/1‑93 (MQ=255)

                                                            gCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGa                             >  2:2007528/1‑93 (MQ=255)

                                                            acAGATGTGGAGATAAAAACATGATAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTGCCAATCCAACCTTCATGa                             <  1:6025042/92‑1 (MQ=255)

                                                              aGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGaac                           <  1:832888/93‑1 (MQ=255)

                                                               gATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGaaca                          >  2:4801150/1‑93 (MQ=255)

                                                               gATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGaaca                          >  1:4985789/1‑93 (MQ=255)

                                                                 tgtgGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGaacaac                        >  2:4217566/1‑93 (MQ=255)

                                                                 tgtgGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGaacaac                        >  2:5527680/1‑93 (MQ=255)

                                                                    ggAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGAt                     <  1:5467950/93‑1 (MQ=255)

                                                                      aaaTAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAg                   <  2:2182403/93‑1 (MQ=255)

                                                                        aTAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCt                 >  1:94090/1‑93 (MQ=255)

                                                                         tAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTa                <  2:5835874/93‑1 (MQ=255)

                                                                         aaaaaaCATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTa                <  2:4641802/92‑1 (MQ=255)

                                                                           aaaaCATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGt              >  1:3654996/1‑93 (MQ=255)

                                                                           aaaaCATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGt              >  1:5550458/1‑93 (MQ=255)

                                                                            aaaCATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTg             >  1:5187324/1‑93 (MQ=255)

                                                                              aCATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCa           >  1:2057866/1‑93 (MQ=255)

                                                                               cATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaa          >  2:3831711/1‑93 (MQ=255)

                                                                               cATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaa          <  1:6104335/93‑1 (MQ=255)

                                                                               cATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGCGCaa          >  1:2976541/1‑93 (MQ=255)

                                                                                aTGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaat         >  2:5785438/1‑93 (MQ=255)

                                                                                aTGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaat         <  2:1546072/93‑1 (MQ=255)

                                                                                aTGAAAACCGCCTGCGAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaat         <  2:1301690/93‑1 (MQ=255)

                                                                                 tGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaata        >  2:6574904/1‑93 (MQ=255)

                                                                                 agAAAACCGCCGGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaata        <  1:1193721/92‑1 (MQ=255)

                                                                                  gAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaataa       <  2:861289/93‑1 (MQ=255)

                                                                                  gAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaataa       <  1:2457023/93‑1 (MQ=255)

                                                                                  gAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaataa       >  1:4005715/1‑93 (MQ=255)

                                                                                  gAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaataa       <  1:5249902/93‑1 (MQ=255)

                                                                                   aaaaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCaataat      <  1:4299236/93‑1 (MQ=255)

                                                                                   aaaaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATCAACAACGATAGCTAGTGCaataat      >  1:6589308/1‑93 (MQ=255)

                                                                                   aaaaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACAAATCCAACCTTCATGAACAACGATAGCTAGTGCaataat      <  1:4061861/93‑1 (MQ=255)

                                                                                    aaaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAAtt     >  2:3753958/1‑93 (MQ=255)

                                                                                     aaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTc    >  2:5538918/1‑93 (MQ=255)

                                                                                     aaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTc    <  2:3644327/93‑1 (MQ=255)

                                                                                     aaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTc    >  1:4768770/1‑93 (MQ=255)

                                                                                     aaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTc    >  1:124979/1‑93 (MQ=255)

                                                                                     aaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTc    <  1:983388/93‑1 (MQ=255)

                                                                                     aaCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTc    >  2:6697278/1‑93 (MQ=255)

                                                                                      aCCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTcc   >  2:5091776/1‑93 (MQ=255)

                                                                                       ccGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCa  <  1:179293/93‑1 (MQ=255)

                                                                                       ccGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCa  <  1:5644310/93‑1 (MQ=255)

GACGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCA  >  NC_000913/2106870‑2107049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAA  >  NC_000913/2106872‑2107053

CGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCT                                                                                          <  SRR4302536.5481101/94‑1 (MQ=60)

 GTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAGCCGCCTGCTA                                                                                         <  SRR4302536.449611/94‑1 (MQ=60)

  TTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAA                                                                                        <  SRR4302536.3141237/94‑1 (MQ=60)

    GGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAG                                                                                      <  SRR4302536.697038/94‑1 (MQ=60)

    GGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAG                                                                                      <  SRR4302536.2299028/94‑1 (MQ=60)

    GGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAG                                                                                      <  SRR4302536.6335374/94‑1 (MQ=60)

    GGTCAAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAG                                                                                      <  SRR4302536.3573598/94‑1 (MQ=60)

      TCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGAT                                                                                    <  SRR4302536.1551579/94‑1 (MQ=60)

      TCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGAT                                                                                    <  SRR4302536.782132/94‑1 (MQ=60)

       CCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATC                                                                                   <  SRR4302536.3392605/94‑1 (MQ=60)

       CCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATC                                                                                   <  SRR4302536.2113617/94‑1 (MQ=60)

          GATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGAC                                                                                <  SRR4302536.1118270/94‑1 (MQ=60)

          GATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGAC                                                                                <  SRR4302536.6096600/94‑1 (MQ=60)

          GATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGAC                                                                                <  SRR4302536.5969458/94‑1 (MQ=60)

           ATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACG                                                                               >  SRR4302536.4335887/1‑94 (MQ=60)

           ATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACG                                                                               >  SRR4302536.5618915/1‑94 (MQ=60)

            TATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGC                                                                              >  SRR4302536.4304202/1‑94 (MQ=60)

             ATAAATCCCCGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCA                                                                             >  SRR4302536.6747094/1‑94 (MQ=60)

             ATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCA                                                                             <  SRR4302536.4275653/94‑1 (MQ=60)

              TAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCAC                                                                            >  SRR4302536.4376441/1‑94 (MQ=60)

               AAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACA                                                                           >  SRR4302536.1309459/1‑94 (MQ=60)

                AATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACAT                                                                          <  SRR4302536.2016782/94‑1 (MQ=60)

                  TCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAGACCGCCTGCTAAAGATCGACGCACATTA                                                                        >  SRR4302536.1410000/1‑94 (MQ=60)

                  TCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTA                                                                        <  SRR4302536.3634793/94‑1 (MQ=60)

                   CCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTAT                                                                       >  SRR4302536.5272392/1‑94 (MQ=60)

                   CCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTAT                                                                       >  SRR4302536.6074563/1‑94 (MQ=60)

                   CCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTAT                                                                       >  SRR4302536.1906595/1‑94 (MQ=60)

                    CCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGGACATTATG                                                                      >  SRR4302536.2631563/1‑94 (MQ=60)

                     CAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGT                                                                     <  SRR4302536.1714386/94‑1 (MQ=60)

                     CAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGT                                                                     >  SRR4302536.1044901/1‑94 (MQ=60)

                      AGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTA                                                                    <  SRR4302536.4960347/94‑1 (MQ=60)

                       GGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAA                                                                   >  SRR4302536.2075874/1‑94 (MQ=60)

                       GGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAA                                                                   >  SRR4302536.4493710/1‑94 (MQ=60)

                        GATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAAT                                                                  <  SRR4302536.1115017/94‑1 (MQ=60)

                        GATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAAT                                                                  >  SRR4302536.3018465/1‑94 (MQ=60)

                          TAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGA                                                                >  SRR4302536.3958905/1‑94 (MQ=60)

                            ATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATA                                                              <  SRR4302536.2275026/94‑1 (MQ=60)

                             TAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAG                                                             >  SRR4302536.5570546/1‑94 (MQ=60)

                             TAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAG                                                             <  SRR4302536.6833012/94‑1 (MQ=60)

                             TAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAG                                                             >  SRR4302536.732421/1‑94 (MQ=60)

                             TAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAG                                                             <  SRR4302536.5037044/94‑1 (MQ=60)

                              AACTATTCCCGTAGAATATAAACTAATAGGAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGC                                                            >  SRR4302536.3469143/1‑94 (MQ=60)

                              AACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGC                                                            >  SRR4302536.6570219/1‑94 (MQ=60)

                              AACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGC                                                            <  SRR4302536.2560299/94‑1 (MQ=60)

                              AACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGC                                                            >  SRR4302536.561407/1‑94 (MQ=60)

                              AACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGC                                                            <  SRR4302536.5578941/94‑1 (MQ=60)

                               ACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGATAAAGATCGACGCACATTATGTAATGATAGCG                                                           <  SRR4302536.3223214/94‑1 (MQ=60)

                                  ATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAA                                                        <  SRR4302536.4341957/94‑1 (MQ=60)

                                  ATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAA                                                        <  SRR4302536.4735339/94‑1 (MQ=60)

                                    TCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACT                                                      <  SRR4302536.6235045/94‑1 (MQ=60)

                                    TCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACT                                                      >  SRR4302536.5743492/1‑94 (MQ=60)

                                      CCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTT                                                    <  SRR4302536.2487398/94‑1 (MQ=60)

                                      CCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTT                                                    <  SRR4302536.766583/94‑1 (MQ=60)

                                       CGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTA                                                   >  SRR4302536.6787618/1‑94 (MQ=60)

                                        GTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTAC                                                  <  SRR4302536.2559884/94‑1 (MQ=60)

                                          AGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCA                                                >  SRR4302536.1572790/1‑94 (MQ=60)

                                          AGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCA                                                >  SRR4302536.4224499/1‑94 (MQ=60)

                                           GAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAA                                               >  SRR4302536.1405576/1‑94 (MQ=60)

                                            AATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAAT                                              <  SRR4302536.5227169/94‑1 (MQ=60)

                                            AATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAAT                                              >  SRR4302536.4887168/1‑94 (MQ=60)

                                             ATATAAACTAATGGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATC                                             >  SRR4302536.2430445/1‑94 (MQ=60)

                                             ATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATC                                             >  SRR4302536.1200532/1‑94 (MQ=60)

                                             ATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATC                                             >  SRR4302536.5045921/1‑94 (MQ=60)

                                               ATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCA                                           >  SRR4302536.2813082/1‑94 (MQ=60)

                                                 AAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAAC                                         >  SRR4302536.1470875/1‑94 (MQ=60)

                                                  AACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACC                                        <  SRR4302536.1322647/94‑1 (MQ=60)

                                                   ACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCT                                       >  SRR4302536.960717/1‑94 (MQ=60)

                                                   ACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCT                                       <  SRR4302536.3194668/94‑1 (MQ=60)

                                                   ACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCT                                       <  SRR4302536.5112007/94‑1 (MQ=60)

                                                   ACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCT                                       >  SRR4302536.2984568/1‑94 (MQ=60)

                                                     TAATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTC                                     >  SRR4302536.2002422/1‑94 (MQ=60)

                                                      AATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCA                                    >  SRR4302536.457217/1‑94 (MQ=60)

                                                      AATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCA                                    <  SRR4302536.2947011/94‑1 (MQ=60)

                                                       ATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCAT                                   <  SRR4302536.6721271/94‑1 (MQ=60)

                                                       ATAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCAT                                   >  SRR4302536.983422/1‑94 (MQ=60)

                                                        TAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATG                                  >  SRR4302536.5344906/1‑94 (MQ=60)

                                                        TAGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATG                                  >  SRR4302536.1939978/1‑94 (MQ=60)

                                                         AGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGA                                 >  SRR4302536.1049544/1‑94 (MQ=60)

                                                         AGCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGA                                 <  SRR4302536.5693447/94‑1 (MQ=60)

                                                          GCAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAA                                <  SRR4302536.4140445/94‑1 (MQ=60)

                                                          ACAGATGTGGAGATAAAAACATGATAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTGCCAATCCAACCTTCATGAA                                <  SRR4302536.6204689/94‑1 (MQ=60)

                                                           CAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAAC                               >  SRR4302536.333421/1‑94 (MQ=60)

                                                           CAGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAAC                               <  SRR4302536.4819736/94‑1 (MQ=60)

                                                            AGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACA                              >  SRR4302536.5135493/1‑94 (MQ=60)

                                                            AGATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACA                              <  SRR4302536.857391/94‑1 (MQ=60)

                                                             GATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAA                             <  SRR4302536.3654495/94‑1 (MQ=60)

                                                              ATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAAC                            >  SRR4302536.6623321/1‑94 (MQ=60)

                                                              ATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAAC                            >  SRR4302536.331117/1‑94 (MQ=60)

                                                              ATGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAAC                            >  SRR4302536.6527570/1‑94 (MQ=60)

                                                               TGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACG                           >  SRR4302536.4214205/1‑94 (MQ=60)

                                                               TGTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACG                           >  SRR4302536.1006936/1‑94 (MQ=60)

                                                                GTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGA                          <  SRR4302536.6117056/94‑1 (MQ=60)

                                                                GTGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGA                          >  SRR4302536.1312176/1‑94 (MQ=60)

                                                                 TGGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGAT                         <  SRR4302536.2027695/94‑1 (MQ=60)

                                                                  GGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATA                        <  SRR4302536.3974169/94‑1 (MQ=60)

                                                                  GGAAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATA                        <  SRR4302536.5631328/94‑1 (MQ=60)

                                                                    AAATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGC                      >  SRR4302536.4507262/1‑94 (MQ=60)

                                                                     AATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCT                     >  SRR4302536.96592/1‑94 (MQ=60)

                                                                     AATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCT                     >  SRR4302536.841256/1‑94 (MQ=60)

                                                                      ATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTA                    <  SRR4302536.1458040/94‑1 (MQ=60)

                                                                      ATAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTA                    >  SRR4302536.1782214/1‑94 (MQ=60)

                                                                       TAAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAG                   <  SRR4302536.4052890/94‑1 (MQ=60)

                                                                        AAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGT                  >  SRR4302536.5715810/1‑94 (MQ=60)

                                                                        AAAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGT                  >  SRR4302536.3764262/1‑94 (MQ=60)

                                                                         AAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTG                 >  SRR4302536.139861/1‑94 (MQ=60)

                                                                         AAAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTG                 >  SRR4302536.5342040/1‑94 (MQ=60)

                                                                          AAACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGC                <  SRR4302536.4366513/94‑1 (MQ=60)

                                                                           AACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCA               >  SRR4302536.2119846/1‑94 (MQ=60)

                                                                           AACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCA               >  SRR4302536.1257004/1‑94 (MQ=60)

                                                                            ACATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGCGCAA              >  SRR4302536.3064160/1‑94 (MQ=60)

                                                                             CATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTGGTGCAAT             <  SRR4302536.4398801/94‑1 (MQ=60)

                                                                             CATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAAT             >  SRR4302536.3211372/1‑94 (MQ=60)

                                                                             CATGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAAT             <  SRR4302536.6286327/94‑1 (MQ=60)

                                                                             CATGAAAACCGCCTGCTAAAGATCCACGGACATTATGTAATGATAGCGTAACTTTACCCATCCAAACTTCATGAACAACGATAGCTCGTGCAAT             >  SRR4302536.2326511/1‑94 (MQ=60)

                                                                              ATGAAAACCACCTGCTAAAGTTCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATA            >  SRR4302536.6767045/1‑94 (MQ=60)

                                                                               TGAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAA           >  SRR4302536.4124580/1‑94 (MQ=60)

                                                                               AGAAAACCGCCGGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAA           <  SRR4302536.1229617/94‑1 (MQ=60)

                                                                                GAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAAT          <  SRR4302536.5406342/94‑1 (MQ=60)

                                                                                GAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAAT          >  SRR4302536.2327416/1‑94 (MQ=60)

                                                                                GAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAAT          <  SRR4302536.2531218/94‑1 (MQ=60)

                                                                                GAAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATCAACAACGATAGCTAGTGCAATAAT          >  SRR4302536.6785326/1‑94 (MQ=60)

                                                                                 AAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATT         <  SRR4302536.2085532/94‑1 (MQ=60)

                                                                                 AAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATT         <  SRR4302536.4427757/94‑1 (MQ=60)

                                                                                 AAAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACAAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATT         <  SRR4302536.4182248/94‑1 (MQ=60)

                                                                                  AAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTC        >  SRR4302536.6164706/1‑94 (MQ=60)

                                                                                  AAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTC        >  SRR4302536.4912374/1‑94 (MQ=60)

                                                                                  AAACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTC        >  SRR4302536.128273/1‑94 (MQ=60)

                                                                                   AACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCC       <  SRR4302536.5907034/94‑1 (MQ=60)

                                                                                   AACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCC       <  SRR4302536.5870350/94‑1 (MQ=60)

                                                                                   AACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCC       >  SRR4302536.1996530/1‑94 (MQ=60)

                                                                                   AACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCC       <  SRR4302536.1012410/94‑1 (MQ=60)

                                                                                    ACCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCA      <  SRR4302536.3100563/94‑1 (MQ=60)

                                                                                     CCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAG     <  SRR4302536.5812007/94‑1 (MQ=60)

                                                                                     CCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAG     <  SRR4302536.3792212/94‑1 (MQ=60)

                                                                                     CCGCCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAG     <  SRR4302536.184031/94‑1 (MQ=60)

                                                                                        CCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAA  >  SRR4302536.5397606/1‑94 (MQ=60)

                                                                                        CCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAA  >  SRR4302536.4095834/1‑94 (MQ=60)

                                                                                        CCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAA  >  SRR4302536.1598869/1‑94 (MQ=60)

CGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAA  >  NC_000913/2106872‑2107053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`