Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,107,690 C→T W157* (TGG→TGA)  rfbC ← dTDP‑6‑deoxy‑D‑glucose‑3,5 epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,107,6900CT99.6% 970.3 / NA 268W157* (TGG→TGA) rfbCdTDP‑6‑deoxy‑D‑glucose‑3,5 epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (135/132);  minor base G (0/1);  total (135/133)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGC  >  NC_000913/2107600‑2107779
                                                                                          |                                                                                         
tATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTca                                                                                         >  1:343970/1‑93 (MQ=255)
 aTTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaa                                                                                        >  2:6120943/1‑93 (MQ=255)
 aTTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaa                                                                                        >  2:5033366/1‑93 (MQ=255)
  ttCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaat                                                                                       <  1:4745397/93‑1 (MQ=255)
     aTGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaatcat                                                                                    >  2:4458651/1‑92 (MQ=255)
      tGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaatcaat                                                                                   >  1:2698564/1‑93 (MQ=255)
      tGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaatcaat                                                                                   <  2:4800841/93‑1 (MQ=255)
       gCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAAtt                                                                                  <  1:4329843/93‑1 (MQ=255)
       gCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAAtt                                                                                  <  2:6280637/93‑1 (MQ=255)
         aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGc                                                                                <  2:6453782/93‑1 (MQ=255)
         aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGc                                                                                >  2:2259254/1‑93 (MQ=255)
         aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGc                                                                                <  1:2182772/93‑1 (MQ=255)
         aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGc                                                                                >  1:2484223/1‑93 (MQ=255)
         aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGc                                                                                >  2:4586474/1‑93 (MQ=255)
           ttaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCaa                                                                              >  2:3129556/1‑93 (MQ=255)
           ttaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCaa                                                                              <  2:5393558/93‑1 (MQ=255)
            taattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAAt                                                                             <  1:2592766/93‑1 (MQ=255)
             aattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAAATGCAATg                                                                            >  2:2738433/1‑93 (MQ=255)
              attTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGc                                                                           >  1:4050944/1‑93 (MQ=255)
               ttttAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCg                                                                          <  2:4332144/93‑1 (MQ=255)
                tttAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGt                                                                         <  1:2974175/93‑1 (MQ=255)
                  tAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtc                                                                       >  2:1356844/1‑93 (MQ=255)
                  tAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtc                                                                       >  1:3858805/1‑93 (MQ=255)
                   aaTCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtca                                                                      <  1:1170931/93‑1 (MQ=255)
                     tCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtcatc                                                                    >  2:3474965/1‑93 (MQ=255)
                       tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCTATTGCAATGCGTTcatcat                                                                  >  2:3990863/1‑93 (MQ=255)
                       tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTcatcat                                                                  >  1:4576557/1‑93 (MQ=255)
                       tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTcatcat                                                                  <  2:1038939/93‑1 (MQ=255)
                          tAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTcc                                                               <  1:6709229/93‑1 (MQ=255)
                            aGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCaa                                                             >  1:2878635/1‑93 (MQ=255)
                            aGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCaa                                                             >  2:633595/1‑93 (MQ=255)
                               tCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACat                                                          >  1:427497/1‑93 (MQ=255)
                               tCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACat                                                          >  2:5197842/1‑93 (MQ=255)
                               tCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACat                                                          >  1:1622717/1‑93 (MQ=255)
                                cATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACata                                                         <  2:1117328/93‑1 (MQ=255)
                                cATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACata                                                         >  1:1009791/1‑93 (MQ=255)
                                 aTCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACatat                                                        <  2:3117229/93‑1 (MQ=255)
                                 aTCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACatat                                                        >  2:2836612/1‑93 (MQ=255)
                                 aTCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACatat                                                        >  2:1508393/1‑93 (MQ=255)
                                 aTCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACatat                                                        >  1:3530136/1‑93 (MQ=255)
                                  tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                       <  1:3060638/93‑1 (MQ=255)
                                  tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                       >  2:6689864/1‑93 (MQ=255)
                                  tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                       <  2:5932407/93‑1 (MQ=255)
                                  tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                       >  1:1005381/1‑93 (MQ=255)
                                     aaCGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCca                                                    >  2:1611362/1‑93 (MQ=255)
                                      aCGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCcac                                                   >  1:3223459/1‑93 (MQ=255)
                                       cGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCcaca                                                  <  2:1526941/93‑1 (MQ=255)
                                         tAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTGCAACATATTCCACAAt                                                >  1:6114254/1‑93 (MQ=255)
                                         tAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAt                                                >  1:3752392/1‑93 (MQ=255)
                                         tAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAt                                                <  2:1317201/93‑1 (MQ=255)
                                          aaaGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATc                                               >  2:1872572/1‑93 (MQ=255)
                                           aaGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCg                                              >  2:873237/1‑93 (MQ=255)
                                           aaGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCg                                              <  2:5622176/93‑1 (MQ=255)
                                           aaGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCg                                              >  1:2059028/1‑93 (MQ=255)
                                             gagCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCt                                            >  2:4262262/1‑93 (MQ=255)
                                               gCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCttt                                          <  1:5249217/93‑1 (MQ=255)
                                                 cTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCa                                        >  1:5461711/1‑93 (MQ=255)
                                                  tttCATCTTTTGGCGAAATAATTAACCCTGATGTATGGGGTCACTCAATTGCAATGCGTTCATCATTCCAACATATCCCACAATCGCTATCAg                                       <  1:3505264/93‑1 (MQ=255)
                                                  tttCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAg                                       <  2:2179926/93‑1 (MQ=255)
                                                  tttCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAg                                       >  1:789570/1‑93 (MQ=255)
                                                   ttCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAgg                                      >  2:4515627/1‑93 (MQ=255)
                                                     cATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgat                                    >  2:6162143/1‑93 (MQ=255)
                                                     cATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgat                                    <  1:3769351/93‑1 (MQ=255)
                                                       tCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatga                                  <  2:1895978/93‑1 (MQ=255)
                                                        cTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatgat                                 >  1:90143/1‑93 (MQ=255)
                                                        cTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatgat                                 <  2:4168998/93‑1 (MQ=255)
                                                        cTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatgat                                 <  2:5564086/93‑1 (MQ=255)
                                                         ttttGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGata                                >  1:1511958/1‑93 (MQ=255)
                                                           ttGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGataat                              >  1:1854870/1‑93 (MQ=255)
                                                            tGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGataata                             <  1:4645804/93‑1 (MQ=255)
                                                               cgcaaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGtt                          <  1:328689/90‑1 (MQ=255)
                                                                 aaaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTg                        <  2:2413686/93‑1 (MQ=255)
                                                                  aaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGt                       <  1:4929747/93‑1 (MQ=255)
                                                                  aaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGt                       >  1:5261595/1‑93 (MQ=255)
                                                                   aGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTa                      <  2:2994011/93‑1 (MQ=255)
                                                                   aGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTa                      >  2:5852046/1‑93 (MQ=255)
                                                                     gATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGt                    <  2:1724788/93‑1 (MQ=255)
                                                                      aTTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGtt                   <  1:1272620/93‑1 (MQ=255)
                                                                       ttAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGttt                  <  2:5781561/93‑1 (MQ=255)
                                                                       ttAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGttt                  <  1:5901352/93‑1 (MQ=255)
                                                                       ttAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGttt                  <  2:1639064/93‑1 (MQ=255)
                                                                          aCCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTtat               >  2:353257/1‑93 (MQ=255)
                                                                           cccTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTtata              >  1:2945777/1‑93 (MQ=255)
                                                                           cccTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTtata              <  1:592314/93‑1 (MQ=255)
                                                                             cTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAtt            <  1:6231327/93‑1 (MQ=255)
                                                                             cTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAtt            <  2:2546578/93‑1 (MQ=255)
                                                                             cTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAtt            <  2:5994124/93‑1 (MQ=255)
                                                                             cTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAtt            <  2:4535410/93‑1 (MQ=255)
                                                                              tGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTg           >  2:686301/1‑93 (MQ=255)
                                                                               gATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGa          >  2:2679003/1‑93 (MQ=255)
                                                                               gATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGa          <  1:577921/93‑1 (MQ=255)
                                                                                aTGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGaa         >  2:3784989/1‑93 (MQ=255)
                                                                                 tGTTTGGGGTGAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGaaa        <  1:6292846/93‑1 (MQ=255)
                                                                                 tGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGaaa        >  2:6529787/1‑93 (MQ=255)
                                                                                  gTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAt       >  1:4413093/1‑93 (MQ=255)
                                                                                  gTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAt       >  2:4740487/1‑93 (MQ=255)
                                                                                   tttGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAtt      <  2:1671129/93‑1 (MQ=255)
                                                                                   tttGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAtt      >  1:83752/1‑93 (MQ=255)
                                                                                    ttGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTc     <  1:2842529/93‑1 (MQ=255)
                                                                                     tGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCa    <  2:5004702/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGC  >  NC_000913/2107600‑2107779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCGATA  >  NC_000913/2107597‑2107783
                                                                                             |                                                                                             
TCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGT                                                                                               <  SRR4302536.3403233/94‑1 (MQ=60)
TCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGT                                                                                               <  SRR4302536.39926/94‑1 (MQ=60)
  GTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCA                                                                                             >  SRR4302536.353091/1‑94 (MQ=60)
  GTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCA                                                                                             <  SRR4302536.3856798/94‑1 (MQ=60)
    ATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAAT                                                                                           <  SRR4302536.3248026/94‑1 (MQ=60)
     TTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATC                                                                                          <  SRR4302536.4888209/94‑1 (MQ=60)
      TCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCA                                                                                         >  SRR4302536.2842878/1‑94 (MQ=60)
      TCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCA                                                                                         >  SRR4302536.1316559/1‑94 (MQ=60)
        ATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAAT                                                                                       >  SRR4302536.3350131/1‑94 (MQ=60)
        ATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAAT                                                                                       >  SRR4302536.2779089/1‑94 (MQ=60)
          GCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTG                                                                                     <  SRR4302536.4459527/94‑1 (MQ=60)
           CAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGC                                                                                    >  SRR4302536.2559175/1‑94 (MQ=60)
           CAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGC                                                                                    >  SRR4302536.3980585/1‑94 (MQ=60)
            AATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCA                                                                                   <  SRR4302536.6686093/94‑1 (MQ=60)
            AATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCA                                                                                   >  SRR4302536.3137667/1‑94 (MQ=60)
            AATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCA                                                                                   <  SRR4302536.2248569/94‑1 (MQ=60)
            AATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCA                                                                                   >  SRR4302536.4411433/1‑94 (MQ=60)
             ATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAA                                                                                  >  SRR4302536.880244/1‑94 (MQ=60)
              TTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAAT                                                                                 <  SRR4302536.2149482/94‑1 (MQ=60)
               TAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATG                                                                                >  SRR4302536.4471846/1‑94 (MQ=60)
               TAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATG                                                                                <  SRR4302536.2670606/94‑1 (MQ=60)
                AATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGC                                                                               >  SRR4302536.4171029/1‑94 (MQ=60)
                 ATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCG                                                                              <  SRR4302536.1079425/94‑1 (MQ=60)
                 ATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCG                                                                              <  SRR4302536.726345/94‑1 (MQ=60)
                 ATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCG                                                                              <  SRR4302536.3716242/94‑1 (MQ=60)
                 ATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCG                                                                              <  SRR4302536.2582993/94‑1 (MQ=60)
                   TTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTT                                                                            <  SRR4302536.3061745/94‑1 (MQ=60)
                    TTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTC                                                                           >  SRR4302536.3973641/1‑94 (MQ=60)
                    TTAAGCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTC                                                                           <  SRR4302536.1652734/94‑1 (MQ=60)
                      AATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCAT                                                                         >  SRR4302536.2624241/1‑94 (MQ=60)
                      AATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCAT                                                                         <  SRR4302536.1302703/94‑1 (MQ=60)
                      AATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCAT                                                                         >  SRR4302536.6350283/1‑94 (MQ=60)
                      AATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCAT                                                                         >  SRR4302536.4697346/1‑94 (MQ=60)
                      AATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCAT                                                                         <  SRR4302536.1206169/94‑1 (MQ=60)
                         CTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCAT                                                                      >  SRR4302536.4713926/1‑94 (MQ=60)
                         CTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCAT                                                                      >  SRR4302536.5762017/1‑94 (MQ=60)
                             TAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCA                                                                  <  SRR4302536.6908440/94‑1 (MQ=60)
                              AAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAA                                                                 >  SRR4302536.2963602/1‑94 (MQ=60)
                               AGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAAC                                                                >  SRR4302536.5616222/1‑94 (MQ=60)
                                GCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACA                                                               <  SRR4302536.721872/94‑1 (MQ=60)
                                 CTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACAT                                                              >  SRR4302536.438750/1‑94 (MQ=60)
                                 CTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACAT                                                              >  SRR4302536.6010779/1‑94 (MQ=60)
                                 CTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACAT                                                              >  SRR4302536.1669976/1‑94 (MQ=60)
                                  TCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATA                                                             >  SRR4302536.1039572/1‑94 (MQ=60)
                                   CATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAT                                                            >  SRR4302536.3635620/1‑94 (MQ=60)
                                    ATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATT                                                           >  SRR4302536.4319219/1‑94 (MQ=60)
                                    ATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATT                                                           >  SRR4302536.1035045/1‑94 (MQ=60)
                                    ATATAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATT                                                           >  SRR4302536.3575205/1‑94 (MQ=60)
                                     TCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTC                                                          <  SRR4302536.3150442/94‑1 (MQ=60)
                                     TCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTC                                                          >  SRR4302536.6398994/1‑94 (MQ=60)
                                      CTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCC                                                         >  SRR4302536.389097/1‑94 (MQ=60)
                                        TACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCAC                                                       <  SRR4302536.4167154/94‑1 (MQ=60)
                                        AACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCAC                                                       >  SRR4302536.3317965/1‑94 (MQ=60)
                                        AACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCAC                                                       >  SRR4302536.823900/1‑94 (MQ=60)
                                           GTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTGCAACATATTCCACAAT                                                    >  SRR4302536.6296487/1‑94 (MQ=60)
                                           GTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAT                                                    >  SRR4302536.3618748/1‑94 (MQ=60)
                                           GTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAT                                                    >  SRR4302536.3864511/1‑94 (MQ=60)
                                           GTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAT                                                    <  SRR4302536.521420/94‑1 (MQ=60)
                                            TAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATC                                                   <  SRR4302536.6292643/94‑1 (MQ=60)
                                             AAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCG                                                  >  SRR4302536.2121037/1‑94 (MQ=60)
                                             AAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCG                                                  <  SRR4302536.5321111/94‑1 (MQ=60)
                                             AAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCG                                                  >  SRR4302536.3340311/1‑94 (MQ=60)
                                               AGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCT                                                <  SRR4302536.2410860/94‑1 (MQ=60)
                                                GAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTT                                               <  SRR4302536.3912421/94‑1 (MQ=60)
                                                 AGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTT                                              <  SRR4302536.6015164/94‑1 (MQ=60)
                                                 AGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTT                                              <  SRR4302536.4609563/94‑1 (MQ=60)
                                                  GCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTC                                             <  SRR4302536.5405637/94‑1 (MQ=60)
                                                   CCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCA                                            >  SRR4302536.3507645/1‑94 (MQ=60)
                                                   CCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCA                                            >  SRR4302536.5624979/1‑94 (MQ=60)
                                                    CTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAG                                           <  SRR4302536.4815646/94‑1 (MQ=60)
                                                    CTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAG                                           >  SRR4302536.818020/1‑94 (MQ=60)
                                                    CTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAG                                           >  SRR4302536.812689/1‑94 (MQ=60)
                                                     TTTCATCTTTTGGCGAAATAATTAACCCTGATGTATGGGGTCACTCAATTGCAATGCGTTCATCATTCCAACATATCCCACAATCGCTATCAGG                                          <  SRR4302536.3609873/94‑1 (MQ=60)
                                                     TTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGG                                          <  SRR4302536.4477254/94‑1 (MQ=60)
                                                     TTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGG                                          >  SRR4302536.1625447/1‑94 (MQ=60)
                                                       TCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGAT                                        <  SRR4302536.210478/94‑1 (MQ=60)
                                                        CATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATG                                       <  SRR4302536.3881892/94‑1 (MQ=60)
                                                         ATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGA                                      >  SRR4302536.3681339/1‑94 (MQ=60)
                                                          TCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGAT                                     >  SRR4302536.92547/1‑94 (MQ=60)
                                                           CTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATA                                    >  SRR4302536.1556488/1‑94 (MQ=60)
                                                           CTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATA                                    >  SRR4302536.5017952/1‑94 (MQ=60)
                                                           CTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATA                                    >  SRR4302536.4944784/1‑94 (MQ=60)
                                                             TTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAAT                                  >  SRR4302536.1910760/1‑94 (MQ=60)
                                                              TTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATA                                 >  SRR4302536.2988195/1‑94 (MQ=60)
                                                               TGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGCTGATAATAG                                >  SRR4302536.3613571/1‑94 (MQ=60)
                                                               TGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAG                                <  SRR4302536.2006532/94‑1 (MQ=60)
                                                               TGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAG                                <  SRR4302536.4785482/94‑1 (MQ=60)
                                                                GGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGT                               <  SRR4302536.3865812/94‑1 (MQ=60)
                                                                 GCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTT                              <  SRR4302536.3294502/94‑1 (MQ=60)
                                                                  CGCAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTT                             <  SRR4302536.337413/94‑1 (MQ=60)
                                                                   GAAAGGATTAACCCTGATGTTTGGTGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTG                            <  SRR4302536.2968916/94‑1 (MQ=60)
                                                                   GAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAGCATATTCCACAATCGCTTTCAGGATGATAATAGTTTG                            <  SRR4302536.486854/94‑1 (MQ=60)
                                                                    AAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGT                           >  SRR4302536.5418403/1‑94 (MQ=60)
                                                                     AAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTA                          >  SRR4302536.4744634/1‑94 (MQ=60)
                                                                     AAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTA                          <  SRR4302536.5077862/94‑1 (MQ=60)
                                                                     AAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTA                          >  SRR4302536.6417286/1‑94 (MQ=60)
                                                                      AGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAG                         >  SRR4302536.1036546/1‑94 (MQ=60)
                                                                         ATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTT                      <  SRR4302536.1310736/94‑1 (MQ=60)
                                                                         ATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTT                      >  SRR4302536.293225/1‑94 (MQ=60)
                                                                          TTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTT                     <  SRR4302536.3082342/94‑1 (MQ=60)
                                                                          TTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTT                     <  SRR4302536.6077096/94‑1 (MQ=60)
                                                                            AACCCTGATGTGTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTAT                   <  SRR4302536.1230079/94‑1 (MQ=60)
                                                                             ACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATA                  >  SRR4302536.3032613/1‑94 (MQ=60)
                                                                             ACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATA                  >  SRR4302536.35447/1‑94 (MQ=60)
                                                                              CCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAT                 <  SRR4302536.2351471/94‑1 (MQ=60)
                                                                              CCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAT                 <  SRR4302536.608192/94‑1 (MQ=60)
                                                                               CCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATT                >  SRR4302536.5410303/1‑94 (MQ=60)
                                                                               CCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATT                <  SRR4302536.6276327/94‑1 (MQ=60)
                                                                               CCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATT                <  SRR4302536.6830615/94‑1 (MQ=60)
                                                                                CTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTG               <  SRR4302536.6416985/94‑1 (MQ=60)
                                                                                CTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTG               >  SRR4302536.2685064/1‑94 (MQ=60)
                                                                                CTGATGTTTGGGGGCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTG               <  SRR4302536.5589279/94‑1 (MQ=60)
                                                                                 TGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGA              >  SRR4302536.4442787/1‑94 (MQ=60)
                                                                                  GATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAA             <  SRR4302536.592593/94‑1 (MQ=60)
                                                                                  GATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAA             >  SRR4302536.6093487/1‑94 (MQ=60)
                                                                                   ATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAA            <  SRR4302536.2723425/94‑1 (MQ=60)
                                                                                    TGTTTGGGGTGAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAT           <  SRR4302536.6480335/94‑1 (MQ=60)
                                                                                    TGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAT           >  SRR4302536.4545159/1‑94 (MQ=60)
                                                                                     GTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATT          <  SRR4302536.2939240/94‑1 (MQ=60)
                                                                                     GTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATT          >  SRR4302536.85998/1‑94 (MQ=60)
                                                                                      TTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTC         >  SRR4302536.1040523/1‑94 (MQ=60)
                                                                                       TTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCA        <  SRR4302536.1693078/94‑1 (MQ=60)
                                                                                       TTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCA        <  SRR4302536.304318/94‑1 (MQ=60)
                                                                                       TTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCA        <  SRR4302536.2926622/94‑1 (MQ=60)
                                                                                        TGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAG       <  SRR4302536.5675496/94‑1 (MQ=60)
                                                                                          GGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCG     <  SRR4302536.1861142/94‑1 (MQ=60)
                                                                                          GGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCG     >  SRR4302536.808137/1‑94 (MQ=60)
                                                                                            GTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCGAT   <  SRR4302536.2787559/94‑1 (MQ=60)
                                                                                             TCAATCAATTGCAATGCGTTGATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCGATA  >  SRR4302536.6666434/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCGATA  >  NC_000913/2107597‑2107783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑