Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,164,290	T→C	Y201H (TAC→CAC)	yegQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,164,290	0	T	C	100.0%	741.1 / NA	228	Y201H (TAC→CAC)	yegQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (108/120); total (108/120)

Only 100 of 242 total aligned reads displayed.

ATGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGTACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTG  >  NC_000913/2164200‑2164379

aTGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCAc                                                                                         <  1:82924/93‑1 (MQ=255)

aTGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCAc                                                                                         >  2:3454171/1‑93 (MQ=255)

  ggCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACaa                                                                                       <  2:3604976/93‑1 (MQ=255)

     cTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGt                                                                                    <  2:4368878/93‑1 (MQ=255)

     cTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGt                                                                                    >  1:1911119/1‑93 (MQ=255)

        cTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCa                                                                                 <  2:1496750/93‑1 (MQ=255)

        cTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCa                                                                                 >  2:4263010/1‑93 (MQ=255)

        cTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCa                                                                                 >  2:3871519/1‑93 (MQ=255)

         tCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAg                                                                                <  2:2948820/93‑1 (MQ=255)

         cccGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAg                                                                                <  1:4854178/92‑1 (MQ=255)

           cGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGa                                                                              <  2:3348042/93‑1 (MQ=255)

            ggTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGaa                                                                             >  2:2267050/1‑93 (MQ=255)

             gTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAGCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAg                                                                            >  2:3560420/1‑93 (MQ=255)

             gTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAg                                                                            <  1:5138615/93‑1 (MQ=255)

             gTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAg                                                                            <  1:4128707/93‑1 (MQ=255)

              tCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAgg                                                                           <  1:2930988/93‑1 (MQ=255)

                gCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGa                                                                         <  1:1345659/93‑1 (MQ=255)

                 ctgcctgcTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGaa                                                                        <  1:3235260/93‑1 (MQ=255)

                  tgcctgcTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGaaa                                                                       >  2:3731551/1‑93 (MQ=255)

                   gcctgcTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAaag                                                                      >  2:3204172/1‑93 (MQ=255)

                   gcctgcTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAaag                                                                      >  1:1739048/1‑93 (MQ=255)

                     ctgcTCTCTGTCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAaagaa                                                                    <  1:961401/93‑1 (MQ=255)

                     ctgcTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAaagaa                                                                    <  2:4812403/93‑1 (MQ=255)

                     ctgcTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAaagaa                                                                    >  2:5782359/1‑93 (MQ=255)

                      tgcTCTCTGGTTATATCACCAAGCGCGACCCGAACCAGGGCACCTGCCCCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAaagaag                                                                   <  2:3444198/93‑1 (MQ=255)

                       cctctctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAagaaga                                                                  <  2:313257/92‑1 (MQ=255)

                        ctctctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAgat                                                                 >  2:1214201/1‑93 (MQ=255)

                        ctctctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAgat                                                                 <  1:429071/93‑1 (MQ=255)

                         tctctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAgatg                                                                >  2:5735650/1‑93 (MQ=255)

                          ctctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAgatga                                                               <  2:1988410/93‑1 (MQ=255)

                          ctctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAgatga                                                               <  2:5632670/93‑1 (MQ=255)

                          ctctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAgatga                                                               <  1:505126/93‑1 (MQ=255)

                           tctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAgatgat                                                              >  2:1392607/1‑93 (MQ=255)

                            ctGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGatgatg                                                             <  1:2805315/93‑1 (MQ=255)

                              ggCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGtt                                                           <  2:4021338/93‑1 (MQ=255)

                               gCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTg                                                          >  1:3414224/1‑93 (MQ=255)

                               gCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTg                                                          >  1:6575990/1‑93 (MQ=255)

                               gCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTg                                                          <  2:6496035/93‑1 (MQ=255)

                                  atatCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCa                                                       >  2:2497385/1‑93 (MQ=255)

                                    atCAACAAGCGCGACCCGAGCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAAc                                                     <  1:3057412/93‑1 (MQ=255)

                                    atCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAAc                                                     <  1:5678472/93‑1 (MQ=255)

                                     tCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACa                                                    <  2:5531730/93‑1 (MQ=255)

                                       aacaaGCGCGACCCGACCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATc                                                  <  1:2848241/93‑1 (MQ=255)

                                       aacaaGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATc                                                  <  1:630848/93‑1 (MQ=255)

                                       aacaaGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATc                                                  <  2:5811169/93‑1 (MQ=255)

                                       aacaaGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATc                                                  >  2:6215495/1‑93 (MQ=255)

                                       aacaaGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATc                                                  >  2:6673042/1‑93 (MQ=255)

                                       aacaaGCGCCACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATc                                                  <  2:2728296/93‑1 (MQ=255)

                                        acaaGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCg                                                 >  2:644526/1‑93 (MQ=255)

                                          aaGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTa                                               >  2:2407392/1‑93 (MQ=255)

                                           aGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTac                                              >  1:4484173/1‑93 (MQ=255)

                                            gcgcGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTaca                                             <  1:5951303/93‑1 (MQ=255)

                                               cgACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACaa                                          <  2:6658388/93‑1 (MQ=255)

                                                gACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAg                                         <  2:340059/93‑1 (MQ=255)

                                                  cccGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTa                                       >  1:4115150/1‑93 (MQ=255)

                                                  cccGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTa                                       <  1:4493257/93‑1 (MQ=255)

                                                  cccGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTa                                       <  2:4755739/93‑1 (MQ=255)

                                                  cccGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTa                                       <  1:6123618/93‑1 (MQ=255)

                                                   ccGAACCGGGCCACCTGCACCACCCCCTGCCTCTGGGAGCACAACGCCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTAc                                      <  1:6149912/93‑1 (MQ=255)

                                                   ccGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTAc                                      <  2:1718915/93‑1 (MQ=255)

                                                    cGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACg                                     <  2:730001/93‑1 (MQ=255)

                                                     gAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGa                                    <  1:4064567/93‑1 (MQ=255)

                                                      aaCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAg                                   >  1:4933324/1‑93 (MQ=255)

                                                      aaCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAg                                   >  2:2599867/1‑93 (MQ=255)

                                                      aaCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAg                                   >  2:522791/1‑93 (MQ=255)

                                                       aCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGc                                  <  1:1045898/93‑1 (MQ=255)

                                                        ccAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGcc                                 <  1:3486496/93‑1 (MQ=255)

                                                         cAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCg                                <  2:2673288/93‑1 (MQ=255)

                                                          aGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGa                               >  2:684827/1‑93 (MQ=255)

                                                          aGGGCACCTGCACCAACCCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGa                               <  2:192288/93‑1 (MQ=255)

                                                            ggCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGAtt                             >  1:2851982/1‑93 (MQ=255)

                                                            ggCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGAtt                             >  2:6111339/1‑93 (MQ=255)

                                                            ggCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGAtt                             <  1:5205984/93‑1 (MQ=255)

                                                              cACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTcc                           >  2:1592572/1‑93 (MQ=255)

                                                               cccTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCg                          <  1:220735/92‑1 (MQ=255)

                                                               aCCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCg                          >  1:712015/1‑93 (MQ=255)

                                                               aCCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCg                          >  1:235999/1‑93 (MQ=255)

                                                                cccgCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCgg                         >  1:5371213/4‑93 (MQ=255)

                                                                ccTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGGAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCgg                         >  1:6698652/1‑93 (MQ=255)

                                                                ccTGCACCAACCCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCgg                         <  1:4633089/93‑1 (MQ=255)

                                                                   gCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGc                      >  2:605373/1‑93 (MQ=255)

                                                                     aCCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCaa                    >  2:369404/1‑93 (MQ=255)

                                                                       cAACGCCTCCCGCTGGGAGCACACCGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCATACACAAGTACGAGCCGATTCCGGTGCaaaa                  <  2:1873746/93‑1 (MQ=255)

                                                                         aCGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATg                >  1:1108295/1‑93 (MQ=255)

                                                                         aCGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATg                >  2:6470058/1‑93 (MQ=255)

                                                                         aCGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATg                >  2:4143041/1‑93 (MQ=255)

                                                                         aCGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATg                <  1:5498423/93‑1 (MQ=255)

                                                                         aCGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATg                >  1:5010145/1‑93 (MQ=255)

                                                                          cGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGt               <  1:2749885/93‑1 (MQ=255)

                                                                           gCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGtt              <  2:5160810/93‑1 (MQ=255)

                                                                            ccTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTg             >  2:2623830/1‑93 (MQ=255)

                                                                              tGCCGCTGGGAGCACAACGTCCAGGAAGGGGAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTGCGGTGGAAAATGTTGAg           >  2:5879166/1‑93 (MQ=255)

                                                                               gCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCTTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGc          <  1:773263/93‑1 (MQ=255)

                                                                                 cGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCg        >  1:6194514/1‑93 (MQ=255)

                                                                                  gCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGa       >  2:4861450/1‑93 (MQ=255)

                                                                                   cTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGAc      <  2:168667/93‑1 (MQ=255)

                                                                                     gggAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGTCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGc    <  2:5581986/93‑1 (MQ=255)

                                                                                      ggAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACAAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCt   <  1:1628287/93‑1 (MQ=255)

                                                                                       gAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTg  >  1:596593/1‑93 (MQ=255)

                                                                                       gAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTg  <  1:4372160/93‑1 (MQ=255)

ATGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGTACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTG  >  NC_000913/2164200‑2164379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCATGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGTACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTGGGT  >  NC_000913/2164198‑2164382

GCATGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCA                                                                                             >  SRR4302536.3937679/1‑94 (MQ=60)

 CATGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCAC                                                                                            <  SRR4302536.765256/94‑1 (MQ=60)

  ATGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACA                                                                                           <  SRR4302536.85150/94‑1 (MQ=60)

   TGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAA                                                                                          <  SRR4302536.860459/94‑1 (MQ=60)

     GCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACG                                                                                        <  SRR4302536.4239551/94‑1 (MQ=60)

     GCCGACTCCGGGCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACG                                                                                        <  SRR4302536.5169695/94‑1 (MQ=60)

      CCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGT                                                                                       >  SRR4302536.1968631/1‑94 (MQ=60)

       CTCCTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGCTCACCTGCCCCAACCCCTGCCGCTGGGAGCACAACGTC                                                                                      <  SRR4302536.1719861/94‑1 (MQ=60)

        TACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCC                                                                                     <  SRR4302536.1902727/94‑1 (MQ=60)

         ACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCA                                                                                    <  SRR4302536.6306422/94‑1 (MQ=60)

         ACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCA                                                                                    >  SRR4302536.3518336/1‑94 (MQ=60)

         ACTCCGGCCGCTGCCTGCGCTCGGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCA                                                                                    <  SRR4302536.3216580/94‑1 (MQ=60)

           TCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGG                                                                                  <  SRR4302536.4684746/94‑1 (MQ=60)

           CCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGG                                                                                  <  SRR4302536.5000249/94‑1 (MQ=60)

              ggacgctcgCTGCTGTCTGGCTAGATCAACAAGCGCGACCCGACCCAGGGCACCTGCACCAACGCCTGCCGCTGGAAGCACAACGTCCAGAAAG                                                                               <  SRR4302536.995919/85‑1 (MQ=60)

              gctccgTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAG                                                                               <  SRR4302536.1893711/88‑1 (MQ=60)

              GGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAG                                                                               >  SRR4302536.3779288/1‑94 (MQ=60)

               GTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGG                                                                              <  SRR4302536.5292011/94‑1 (MQ=60)

               GTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGG                                                                              <  SRR4302536.4251109/94‑1 (MQ=60)

                TCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGG                                                                             <  SRR4302536.3017473/94‑1 (MQ=60)

                TCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGG                                                                             <  SRR4302536.2920831/94‑1 (MQ=60)

                 CGCTGCCTGCTCTCTGGCTATATCAACCAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGA                                                                            >  SRR4302536.3412278/1‑94 (MQ=60)

                 CGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGA                                                                            <  SRR4302536.1543846/94‑1 (MQ=60)

                  GCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAA                                                                           <  SRR4302536.1385775/94‑1 (MQ=60)

                   CTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAA                                                                          <  SRR4302536.3330095/94‑1 (MQ=60)

                    TGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAG                                                                         >  SRR4302536.1789233/1‑94 (MQ=60)

                     GCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGA                                                                        >  SRR4302536.5829430/1‑94 (MQ=60)

                      CCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACGAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAA                                                                       >  SRR4302536.5975794/1‑94 (MQ=60)

                      CCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAA                                                                       >  SRR4302536.2933589/1‑94 (MQ=60)

                      CCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAA                                                                       >  SRR4302536.3002077/1‑94 (MQ=60)

                       CTGCTCTCTGTCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAG                                                                      <  SRR4302536.989816/94‑1 (MQ=60)

                       CTGCTCTCTGGCTATATCACCAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAG                                                                      <  SRR4302536.6163197/94‑1 (MQ=60)

                         GCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGAT                                                                    <  SRR4302536.6856890/94‑1 (MQ=60)

                          CTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATG                                                                   <  SRR4302536.440366/94‑1 (MQ=60)

                          CTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATG                                                                   <  SRR4302536.1094513/94‑1 (MQ=60)

                            CTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGAT                                                                 <  SRR4302536.6133286/94‑1 (MQ=60)

                            CTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGAT                                                                 <  SRR4302536.518096/94‑1 (MQ=60)

                             TCTGGCTATATCAACAAGCGCGACCCGAACCAGGTCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATG                                                                <  SRR4302536.723127/94‑1 (MQ=60)

                              CTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGT                                                               <  SRR4302536.2888509/94‑1 (MQ=60)

                               TGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTT                                                              >  SRR4302536.5587383/1‑94 (MQ=60)

                               TGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTT                                                              <  SRR4302536.2598706/94‑1 (MQ=60)

                                GGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTG                                                             >  SRR4302536.749076/1‑94 (MQ=60)

                                GGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTG                                                             >  SRR4302536.3515813/1‑94 (MQ=60)

                                GGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTG                                                             >  SRR4302536.6771701/1‑94 (MQ=60)

                                GGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTG                                                             >  SRR4302536.4374/1‑94 (MQ=60)

                                  CTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGC                                                           >  SRR4302536.4972407/1‑94 (MQ=60)

                                   TATATCAACAAGCGCGCCCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCA                                                          <  SRR4302536.1720234/94‑1 (MQ=60)

                                   TATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCA                                                          <  SRR4302536.225994/94‑1 (MQ=60)

                                    ATATCAACAAGCGCGACCCGACCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAA                                                         <  SRR4302536.4724767/94‑1 (MQ=60)

                                      ATCAACAAGCGCGACCCGAGCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACA                                                       <  SRR4302536.3147168/94‑1 (MQ=60)

                                      ATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACA                                                       <  SRR4302536.5847303/94‑1 (MQ=60)

                                         AACAAGCGCGACCCGACCCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCG                                                    <  SRR4302536.2932464/94‑1 (MQ=60)

                                         AACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCG                                                    >  SRR4302536.6478743/1‑94 (MQ=60)

                                         AACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCG                                                    <  SRR4302536.649525/94‑1 (MQ=60)

                                          ACAAGCGCGCCCCGAACCAGGGAACCTGCACCAACGCCTGCCGCTCGGAACACAACGTCCAcaaagggctagaagaggatagtgtcatcatcgt                                                   >  SRR4302536.2842343/1‑61 (MQ=39)

                                            AAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTAC                                                 >  SRR4302536.4618856/1‑94 (MQ=60)

                                            AAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTAC                                                 >  SRR4302536.4618855/1‑94 (MQ=60)

                                             AGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACA                                                >  SRR4302536.6484672/1‑94 (MQ=60)

                                              GCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACAC                                               <  SRR4302536.6128594/94‑1 (MQ=60)

                                              GCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGAAGATGTTGGCAATATCGTACAC                                               <  SRR4302536.6768052/94‑1 (MQ=60)

                                               CGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACA                                              >  SRR4302536.5950499/1‑94 (MQ=60)

                                               CGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACA                                              >  SRR4302536.2920031/1‑94 (MQ=60)

                                                 CGCCCCGAACCAGGGCACCTGCACCAACGCCTGCAGCTGGGAGCACAAGGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAG                                            <  SRR4302536.1876615/94‑1 (MQ=60)

                                                  GACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGA                                           >  SRR4302536.2479643/1‑94 (MQ=60)

                                                   TCCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTA                                          >  SRR4302536.434372/1‑94 (MQ=60)

                                                   ACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTA                                          >  SRR4302536.4237211/1‑94 (MQ=60)

                                                    CCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTAC                                         <  SRR4302536.4628162/94‑1 (MQ=60)

                                                    CCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTAC                                         >  SRR4302536.5213092/1‑94 (MQ=60)

                                                    CCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTAC                                         <  SRR4302536.6306119/94‑1 (MQ=60)

                                                    CCCGAACCAGGGCACCTACACCAGCGCCTGCCTCTGGAAGCACAACGCCCAGGAAGGGAAAGAAGATGATctgggtaacggcgtactcaagtag                                         <  SRR4302536.6629011/94‑25 (MQ=44)

                                                     CCGAACCGGGCCACCTGCACCACCCCCTGCCTCTGGGAGCACAACGCCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACG                                        <  SRR4302536.6333141/94‑1 (MQ=60)

                                                     CCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACG                                        >  SRR4302536.1390842/1‑94 (MQ=60)

                                                       GAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAG                                      <  SRR4302536.4185033/94‑1 (MQ=60)

                                                       GAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAG                                      >  SRR4302536.5081544/1‑94 (MQ=60)

                                                       GAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAG                                      >  SRR4302536.5159455/1‑94 (MQ=60)

                                                        AACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGC                                     <  SRR4302536.4560273/94‑1 (MQ=60)

                                                         ACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCC                                    <  SRR4302536.1077020/94‑1 (MQ=60)

                                                          CCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCG                                   <  SRR4302536.3590483/94‑1 (MQ=60)

                                                           CAGGCCACCTGCACAAACCCGTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGA                                  <  SRR4302536.2312742/94‑1 (MQ=60)

                                                            AGGGCCCCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGAT                                 <  SRR4302536.5044226/94‑1 (MQ=60)

                                                            AGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGAT                                 >  SRR4302536.1155229/1‑94 (MQ=60)

                                                            AGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGAT                                 >  SRR4302536.4119271/1‑94 (MQ=60)

                                                            AGGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGAT                                 >  SRR4302536.4507509/1‑94 (MQ=60)

                                                             GGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATT                                <  SRR4302536.3354473/94‑1 (MQ=60)

                                                             GGGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATT                                >  SRR4302536.2936285/1‑94 (MQ=60)

                                                              GGCACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTC                               <  SRR4302536.5361163/94‑1 (MQ=60)

                                                                CACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCG                             >  SRR4302536.732820/1‑94 (MQ=60)

                                                                CACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCG                             >  SRR4302536.242269/1‑94 (MQ=60)

                                                                CACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCG                             <  SRR4302536.628705/94‑1 (MQ=60)

                                                                 CCCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGG                            <  SRR4302536.226592/94‑1 (MQ=60)

                                                                 CCCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGG                            <  SRR4302536.765612/94‑1 (MQ=60)

                                                                 ACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGGAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGG                            >  SRR4302536.6897617/1‑94 (MQ=60)

                                                                 ACCTGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGG                            >  SRR4302536.2843523/1‑94 (MQ=60)

                                                                 ACCCGCACCAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGG                            >  SRR4302536.5531447/1‑94 (MQ=60)

                                                                  CCTGCACCAACCCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGT                           <  SRR4302536.4772323/94‑1 (MQ=60)

                                                                         CAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAAT                    <  SRR4302536.837184/94‑1 (MQ=60)

                                                                         CAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAAT                    >  SRR4302536.5281022/1‑94 (MQ=60)

                                                                         CAACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAAT                    <  SRR4302536.5408026/94‑1 (MQ=60)

                                                                         CAACCCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAAT                    <  SRR4302536.3011719/94‑1 (MQ=60)

                                                                          AACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATG                   >  SRR4302536.5160420/1‑94 (MQ=60)

                                                                          AACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATG                   >  SRR4302536.1141547/1‑94 (MQ=60)

                                                                           ACGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGT                  <  SRR4302536.5662488/94‑1 (MQ=60)

                                                                            CGCCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTT                 <  SRR4302536.2831766/94‑1 (MQ=60)

                                                                              CCTGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGA               >  SRR4302536.3088724/1‑94 (MQ=60)

                                                                                TGCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGC             <  SRR4302536.4459268/94‑1 (MQ=60)

                                                                                 GCCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCTTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCC            <  SRR4302536.795931/94‑1 (MQ=60)

                                                                                  CCGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCG           >  SRR4302536.6379025/1‑94 (MQ=60)

                                                                                   CGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGA          >  SRR4302536.528414/1‑94 (MQ=60)

                                                                                   CGCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGA          >  SRR4302536.6876394/1‑94 (MQ=60)

                                                                                    GCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGAC         >  SRR4302536.635564/1‑94 (MQ=60)

                                                                                    GCTGGGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGAC         <  SRR4302536.4227027/94‑1 (MQ=60)

                                                                                    GCTGGGAGCACAACGCCCAGGAAGGGAAAGAAGAGGATGTGGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGAC         <  SRR4302536.2000075/94‑1 (MQ=60)

                                                                                        GGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTG     >  SRR4302536.5151227/1‑94 (MQ=60)

                                                                                        GGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTG     >  SRR4302536.613247/1‑94 (MQ=60)

                                                                                        GGAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACAAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTG     <  SRR4302536.1675686/94‑1 (MQ=60)

                                                                                         GAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTGG    <  SRR4302536.5369371/94‑1 (MQ=60)

                                                                                         GAGCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTGG    <  SRR4302536.4502756/94‑1 (MQ=60)

                                                                                          AGCACAACGTCCAGGAAGGAAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTGGG   <  SRR4302536.2503292/94‑1 (MQ=60)

                                                                                           GCACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTGGGT  >  SRR4302536.1018605/1‑94 (MQ=60)

                                                                                           GCACAACGTCAAGGAAGGAAAAGAAGAGAATGTGGGCAACACCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTGGGT  <  SRR4302536.1521189/94‑1 (MQ=60)

GCATGGCCTACTCCGGTCGCTGCCTGCTCTCTGGCTATATCAACAAGCGCGACCCGAACCAGGGCACCTGCACCAACGCCTGCCGCTGGGAGTACAACGTCCAGGAAGGGAAAGAAGATGATGTTGGCAACATCGTACACAAGTACGAGCCGATTCCGGTGCAAAATGTTGAGCCGACGCTGGGT  >  NC_000913/2164198‑2164382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`