Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,299,266 T→C N503S (AAC→AGC)  napA ← putative nitrate reductase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,299,2660TC99.6% 723.2 / NA 223N503S (AAC→AGC) napAputative nitrate reductase 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (87/135);  minor base G (1/0);  total (88/135)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355
                                                                                          |                                                                                         
cccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTg                                                                                         >  2:1768773/1‑93 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgc                                                                                        >  2:3836682/1‑93 (MQ=255)
  ctctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcg                                                                                       <  1:1156506/93‑1 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      <  2:1166686/93‑1 (MQ=255)
    ctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGcgcg                                                                                     >  2:3029317/1‑93 (MQ=255)
    ctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGcgcg                                                                                     >  2:1545363/1‑93 (MQ=255)
     tgtctcTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCgg                                                                                    <  2:4924618/91‑1 (MQ=255)
      ttCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGa                                                                                   <  1:2571099/93‑1 (MQ=255)
       tctcTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAt                                                                                  <  1:518023/93‑1 (MQ=255)
        ctctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATc                                                                                 <  2:2391872/93‑1 (MQ=255)
         tctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcg                                                                                >  2:5986078/1‑93 (MQ=255)
          ctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcgc                                                                               >  2:6606112/1‑93 (MQ=255)
             cccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGcc                                                                            <  1:2106671/93‑1 (MQ=255)
             cccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGcc                                                                            <  1:3862690/93‑1 (MQ=255)
             cccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGcc                                                                            >  2:1444643/1‑93 (MQ=255)
                acaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGc                                                                         >  2:2450558/1‑93 (MQ=255)
                acaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGc                                                                         >  2:3473988/1‑93 (MQ=255)
                acaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGc                                                                         >  1:2595447/1‑93 (MQ=255)
                 caTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGcc                                                                        >  2:5394437/1‑93 (MQ=255)
                 caTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGcc                                                                        <  2:5183641/93‑1 (MQ=255)
                  aTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGccc                                                                       <  2:1381262/93‑1 (MQ=255)
                   ttGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCg                                                                      >  1:1253718/1‑93 (MQ=255)
                   ttGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCg                                                                      >  1:4543314/1‑93 (MQ=255)
                    tGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCgg                                                                     <  1:3683096/93‑1 (MQ=255)
                      cgctCGGCAAGATCAATTGTGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCa                                                                   <  1:4154769/90‑1 (MQ=255)
                        gTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATa                                                                 <  1:1349740/93‑1 (MQ=255)
                             cAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctc                                                            <  1:285552/93‑1 (MQ=255)
                             cAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctc                                                            >  2:2165079/1‑93 (MQ=255)
                              aGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctct                                                           <  1:72281/93‑1 (MQ=255)
                              aGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctct                                                           >  1:4441686/1‑93 (MQ=255)
                               ggATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCtt                                                          >  2:4331963/1‑93 (MQ=255)
                               ggATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCtt                                                          <  1:4323879/93‑1 (MQ=255)
                                gATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTc                                                         <  2:2219930/93‑1 (MQ=255)
                                gATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTc                                                         <  1:5833819/93‑1 (MQ=255)
                                  tCAAGTCGGCGGCCAGCGCTCTGACTGTCGGATCCGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGTCATACGCTCTTCAt                                                       <  1:2641941/93‑1 (MQ=255)
                                  tCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAt                                                       >  1:1388479/1‑93 (MQ=255)
                                   cAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAtt                                                      <  2:3924269/93‑1 (MQ=255)
                                    aaGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTg                                                     >  1:479411/1‑93 (MQ=255)
                                    aaGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTg                                                     >  1:1695818/1‑93 (MQ=255)
                                     aGTCGGCGGCCAGCGCACTGACTGTCGGATCCGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGa                                                    <  1:6376440/93‑1 (MQ=255)
                                     aGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGa                                                    <  1:5812415/93‑1 (MQ=255)
                                     aGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGa                                                    <  1:2365294/93‑1 (MQ=255)
                                      gTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATGGCGCCCGCCCCGCGTAGCGTCTTCCTTGAt                                                   >  1:69596/1‑93 (MQ=255)
                                      gTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAt                                                   <  1:4812464/93‑1 (MQ=255)
                                       tCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATg                                                  >  1:5044582/1‑93 (MQ=255)
                                        cggcggCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGt                                                 <  2:3132034/93‑1 (MQ=255)
                                        cggcggCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGt                                                 <  2:6275706/93‑1 (MQ=255)
                                         ggcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGtt                                                <  1:1813900/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  2:5826095/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  1:6407744/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               >  2:436507/1‑93 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  1:267050/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  2:3599768/93‑1 (MQ=255)
                                           cggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcg                                              <  2:6108013/93‑1 (MQ=255)
                                              ccAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcc                                           <  1:5074954/93‑1 (MQ=255)
                                              ccAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcc                                           >  2:6397599/1‑93 (MQ=255)
                                               cAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggccc                                          <  1:4051386/93‑1 (MQ=255)
                                               cAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggccc                                          <  1:5008055/93‑1 (MQ=255)
                                                aGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccg                                         >  1:5637833/1‑93 (MQ=255)
                                                aGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGACAGCCCGGCATACGCTCTTCATTGATGTTcggcccg                                         >  2:5540569/1‑93 (MQ=255)
                                                 gcgcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCTCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccgg                                        <  1:3409784/93‑1 (MQ=255)
                                                  ggcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggc                                       <  1:1479871/92‑1 (MQ=255)
                                                   gcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccgggc                                      >  1:601260/1‑91 (MQ=255)
                                                   gcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggcc                                      >  1:5414557/1‑93 (MQ=255)
                                                   gcACTGACTGTCGGATACGGATCGGAGACGATGAAGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggcc                                      <  1:2065728/93‑1 (MQ=255)
                                                     actgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTg                                    >  1:896507/1‑93 (MQ=255)
                                                      ctgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGc                                   <  1:5717936/93‑1 (MQ=255)
                                                      ctgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGc                                   >  2:3362789/1‑93 (MQ=255)
                                                         actgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtg                                >  1:3855316/1‑93 (MQ=255)
                                                         actgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtg                                >  2:6682593/1‑93 (MQ=255)
                                                          ctgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgt                               <  2:1061018/93‑1 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              <  1:1053656/93‑1 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              <  1:5978198/93‑1 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             >  1:3036027/1‑93 (MQ=255)
                                                              cGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttgtc                           >  1:792620/1‑92 (MQ=255)
                                                              cGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATgttgtt                           >  2:2658506/1‑93 (MQ=255)
                                                               ggATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGttgttg                          >  2:2028851/1‑93 (MQ=255)
                                                               ggATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGttgttg                          <  2:6170652/93‑1 (MQ=255)
                                                                 aTACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC‑GCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTa                       >  1:2193130/1‑93 (MQ=255)
                                                                  tACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTa                       <  2:6492517/93‑1 (MQ=255)
                                                                  tACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTa                       >  1:3319904/1‑93 (MQ=255)
                                                                    cGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTaca                     <  2:2678817/93‑1 (MQ=255)
                                                                    cGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTaca                     <  2:6431557/93‑1 (MQ=255)
                                                                      gATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAcaca                   <  2:78860/93‑1 (MQ=255)
                                                                      gATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAcaca                   >  2:68709/1‑93 (MQ=255)
                                                                      gATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAcaca                   <  2:110284/93‑1 (MQ=255)
                                                                      gATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAcaca                   <  1:4881896/93‑1 (MQ=255)
                                                                       aTCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAt                  <  2:3246533/93‑1 (MQ=255)
                                                                       aTCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAt                  <  1:2749519/93‑1 (MQ=255)
                                                                        tCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATg                 >  2:1398977/1‑93 (MQ=255)
                                                                          ggAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGt               <  2:4229895/93‑1 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              >  1:4231031/1‑93 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              >  2:5634804/1‑93 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              <  1:354580/93‑1 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              >  1:6217050/1‑93 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              <  1:5703346/93‑1 (MQ=255)
                                                                            agaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTcc             >  1:5240465/1‑93 (MQ=255)
                                                                             gaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            >  1:1828017/1‑93 (MQ=255)
                                                                             gaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            <  1:2938508/93‑1 (MQ=255)
                                                                                     tgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAt    >  2:1750236/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAG  >  NC_000913/2299173‑2299357
                                                                                             |                                                                                           
GCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGC                                                                                             >  SRR4302536.923549/1‑94 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            <  SRR4302536.4518167/94‑1 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            <  SRR4302536.2815666/94‑1 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            >  SRR4302536.6208115/1‑94 (MQ=60)
  GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTG                                                                                           <  SRR4302536.5945873/94‑1 (MQ=60)
  GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTG                                                                                           >  SRR4302536.6518883/1‑94 (MQ=60)
   CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGC                                                                                          >  SRR4302536.3282264/1‑94 (MQ=60)
   CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGC                                                                                          <  SRR4302536.5320222/94‑1 (MQ=60)
    CCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCG                                                                                         <  SRR4302536.1009236/94‑1 (MQ=60)
     CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGC                                                                                        <  SRR4302536.1191315/94‑1 (MQ=60)
     CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGC                                                                                        >  SRR4302536.2839011/1‑94 (MQ=60)
     CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGC                                                                                        >  SRR4302536.4919817/1‑94 (MQ=60)
     CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGC                                                                                        >  SRR4302536.2155369/1‑94 (MQ=60)
      TCTTTCTCTACCCACATTGCGGTCGGCAGCATCGAGTCGGCGGCCAGCGCGCTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCG                                                                                       <  SRR4302536.2330209/94‑1 (MQ=60)
        TTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGA                                                                                     >  SRR4302536.3750081/1‑94 (MQ=60)
         TTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAT                                                                                    <  SRR4302536.2648422/94‑1 (MQ=60)
          TCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATC                                                                                   <  SRR4302536.2702115/94‑1 (MQ=60)
          TCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATC                                                                                   <  SRR4302536.531308/94‑1 (MQ=60)
           CTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCG                                                                                  <  SRR4302536.2731164/94‑1 (MQ=60)
           CTCTACCCACATTGCGGTCGGCAGCATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCG                                                                                  <  SRR4302536.6595813/94‑1 (MQ=60)
              TACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGC                                                                               <  SRR4302536.1367526/94‑1 (MQ=60)
               ACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCC                                                                              <  SRR4302536.211135/94‑1 (MQ=60)
                CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                             <  SRR4302536.1307474/94‑1 (MQ=60)
                CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                             >  SRR4302536.516440/1‑94 (MQ=60)
                CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                             <  SRR4302536.5206476/94‑1 (MQ=60)
                CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                             <  SRR4302536.3977631/94‑1 (MQ=60)
                CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                             <  SRR4302536.2169980/94‑1 (MQ=60)
                CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                             <  SRR4302536.3426761/94‑1 (MQ=60)
                 CCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTTGGATACGGATCGGAGACGATGATGAAGCTGCGCAGCTCGCGCAAG                                                                            >  SRR4302536.2400031/1‑94 (MQ=60)
                 CCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAG                                                                            <  SRR4302536.6791456/94‑1 (MQ=60)
                 CCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCAGATCGCGCCAG                                                                            <  SRR4302536.1605635/94‑1 (MQ=60)
                  CACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGC                                                                           >  SRR4302536.2673378/1‑94 (MQ=60)
                  CACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGC                                                                           >  SRR4302536.363520/1‑94 (MQ=60)
                   ACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCC                                                                          >  SRR4302536.381158/1‑94 (MQ=60)
                    CATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC                                                                         >  SRR4302536.1903024/1‑94 (MQ=60)
                     ATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCG                                                                        >  SRR4302536.1291354/1‑94 (MQ=60)
                     ATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCG                                                                        >  SRR4302536.5854825/1‑94 (MQ=60)
                     ATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCG                                                                        >  SRR4302536.4679555/1‑94 (MQ=60)
                       TGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGC                                                                      <  SRR4302536.3793206/94‑1 (MQ=60)
                        GCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCA                                                                     <  SRR4302536.5373703/94‑1 (MQ=60)
                         cgctcggcaagatcaattgtGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAT                                                                    <  SRR4302536.4278076/74‑1 (MQ=60)
                           GTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAC                                                                  <  SRR4302536.1389997/94‑1 (MQ=60)
                            TCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCCGAGACGATGATGAAGCTGCGCGGATCGCGCCAGACCGGCATACG                                                                 >  SRR4302536.3640706/1‑94 (MQ=60)
                             CGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATTAAGCTGCGCGGATCGCGGCCGCCCGGGATCCGC                                                                >  SRR4302536.5404333/1‑94 (MQ=60)
                              GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                               <  SRR4302536.2951713/94‑1 (MQ=60)
                                CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                             >  SRR4302536.5946266/1‑94 (MQ=60)
                                CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                             >  SRR4302536.4575036/1‑94 (MQ=60)
                                CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                             <  SRR4302536.293147/94‑1 (MQ=60)
                                CAGAATCAAGTCGGAGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                             <  SRR4302536.4423836/94‑1 (MQ=60)
                                 AGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTT                                                            <  SRR4302536.793267/94‑1 (MQ=60)
                                 AGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTT                                                            <  SRR4302536.5884401/94‑1 (MQ=60)
                                 AGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTT                                                            <  SRR4302536.74236/94‑1 (MQ=60)
                                  GGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTC                                                           <  SRR4302536.4453359/94‑1 (MQ=60)
                                  GGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTC                                                           <  SRR4302536.1184616/94‑1 (MQ=60)
                                   GATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCA                                                          <  SRR4302536.6007642/94‑1 (MQ=60)
                                    ATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAT                                                         >  SRR4302536.1429826/1‑94 (MQ=60)
                                     TCAAGTCGGCGGCCAGCGCTCTGACTGTCGGATCCGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGTCATACGCTCTTCATT                                                        <  SRR4302536.2721120/94‑1 (MQ=60)
                                      CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTG                                                       >  SRR4302536.491886/1‑94 (MQ=60)
                                      CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTG                                                       <  SRR4302536.4199508/94‑1 (MQ=60)
                                      CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTG                                                       >  SRR4302536.1744906/1‑94 (MQ=60)
                                      CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTG                                                       <  SRR4302536.1426149/94‑1 (MQ=60)
                                        AGTCGGCGGCCAGCGCACTGACTGTCGGATCCGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                     <  SRR4302536.6566486/94‑1 (MQ=60)
                                        AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATGGCGCCcgccccgcgtagcgtcttccttgat                                                     >  SRR4302536.71481/1‑69 (MQ=60)
                                        AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                     <  SRR4302536.5985547/94‑1 (MQ=60)
                                        AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                     <  SRR4302536.1208389/94‑1 (MQ=60)
                                        AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                     <  SRR4302536.2436985/94‑1 (MQ=60)
                                         GTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                    >  SRR4302536.5195759/1‑94 (MQ=60)
                                         GTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                    <  SRR4302536.4957273/94‑1 (MQ=60)
                                         ATCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                    <  SRR4302536.1870128/94‑1 (MQ=60)
                                           CGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTT                                                  >  SRR4302536.2662945/1‑94 (MQ=60)
                                           CGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTT                                                  <  SRR4302536.292983/94‑1 (MQ=60)
                                            GGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTC                                                 <  SRR4302536.1868622/94‑1 (MQ=60)
                                            GGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTC                                                 <  SRR4302536.6436959/94‑1 (MQ=60)
                                            GGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTC                                                 >  SRR4302536.740099/1‑94 (MQ=60)
                                             GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                <  SRR4302536.3081635/94‑1 (MQ=60)
                                             GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                <  SRR4302536.274173/94‑1 (MQ=60)
                                             GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                <  SRR4302536.6599115/94‑1 (MQ=60)
                                              CGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGG                                               <  SRR4302536.2684422/94‑1 (MQ=60)
                                               GGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGC                                              >  SRR4302536.4403397/1‑94 (MQ=60)
                                                 CCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCC                                            <  SRR4302536.5226873/94‑1 (MQ=60)
                                                  CAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCG                                           >  SRR4302536.6188277/1‑94 (MQ=60)
                                                  CAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCG                                           <  SRR4302536.4171491/94‑1 (MQ=60)
                                                  CAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCG                                           >  SRR4302536.5805326/1‑94 (MQ=60)
                                                  CAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCG                                           <  SRR4302536.5158278/94‑1 (MQ=60)
                                                   AGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGG                                          >  SRR4302536.2785118/1‑94 (MQ=60)
                                                   AGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGG                                          >  SRR4302536.4957499/1‑94 (MQ=60)
                                                    GCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCTCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGC                                         <  SRR4302536.3511215/94‑1 (MQ=60)
                                                     GGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                        <  SRR4302536.1523612/94‑1 (MQ=60)
                                                     CGCACTGACTGTCGGATACGGATCGGCGACGGTGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                        >  SRR4302536.563974/1‑94 (MQ=60)
                                                     CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGGC                                        >  SRR4302536.618739/1‑94 (MQ=60)
                                                     CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                        >  SRR4302536.5576375/1‑94 (MQ=60)
                                                      GCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCT                                       <  SRR4302536.5959579/94‑1 (MQ=60)
                                                      GCACTGACTGTCGGATACGGATCGGAGACGATGAAGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCT                                       <  SRR4302536.2127954/94‑1 (MQ=60)
                                                       CACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTG                                      <  SRR4302536.5998095/94‑1 (MQ=60)
                                                       CACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTG                                      <  SRR4302536.4304672/94‑1 (MQ=60)
                                                       CACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTG                                      >  SRR4302536.922921/1‑94 (MQ=60)
                                                         CTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCA                                    <  SRR4302536.5887969/94‑1 (MQ=60)
                                                         CTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCA                                    >  SRR4302536.5132425/1‑94 (MQ=60)
                                                          TGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAT                                   <  SRR4302536.2460920/94‑1 (MQ=60)
                                                           GACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATG                                  >  SRR4302536.3970061/1‑94 (MQ=60)
                                                            ACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGT                                 <  SRR4302536.3973684/94‑1 (MQ=60)
                                                             CTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTT                                <  SRR4302536.5578063/94‑1 (MQ=60)
                                                              TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                               >  SRR4302536.3125273/1‑94 (MQ=60)
                                                              TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                               <  SRR4302536.6156448/94‑1 (MQ=60)
                                                              TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                               <  SRR4302536.1085134/94‑1 (MQ=60)
                                                               GTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGT                              >  SRR4302536.4707994/1‑94 (MQ=60)
                                                                TCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTT                             <  SRR4302536.6160489/94‑1 (MQ=60)
                                                                TCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTT                             <  SRR4302536.6386117/94‑1 (MQ=60)
                                                                TCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTC                             >  SRR4302536.815855/1‑94 (MQ=60)
                                                                  GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                           >  SRR4302536.1444005/1‑94 (MQ=60)
                                                                  GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                           >  SRR4302536.5255510/1‑94 (MQ=60)
                                                                   GATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC‑GCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTA                         >  SRR4302536.2259290/1‑94 (MQ=60)
                                                                    ATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTA                         >  SRR4302536.3418043/1‑94 (MQ=60)
                                                                      ACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACA                       >  SRR4302536.6120113/1‑94 (MQ=60)
                                                                      ACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACA                       >  SRR4302536.4576584/1‑94 (MQ=60)
                                                                         GATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAT                    <  SRR4302536.5028771/94‑1 (MQ=60)
                                                                          ATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATG                   <  SRR4302536.406904/94‑1 (MQ=60)
                                                                          ATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATG                   <  SRR4302536.2831390/94‑1 (MQ=60)
                                                                           TCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGG                  <  SRR4302536.3156508/94‑1 (MQ=60)
                                                                           TCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGG                  <  SRR4302536.3770282/94‑1 (MQ=60)
                                                                           TCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGG                  <  SRR4302536.5039412/94‑1 (MQ=60)
                                                                            CGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGT                 >  SRR4302536.1669807/1‑94 (MQ=60)
                                                                             GGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTC                >  SRR4302536.4356932/1‑94 (MQ=60)
                                                                             GGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTC                >  SRR4302536.6402311/1‑94 (MQ=60)
                                                                              GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC               <  SRR4302536.363960/94‑1 (MQ=60)
                                                                              GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC               >  SRR4302536.5396603/1‑94 (MQ=60)
                                                                              GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC               <  SRR4302536.5872937/94‑1 (MQ=60)
                                                                               AGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCA              <  SRR4302536.1824081/94‑1 (MQ=60)
                                                                               AGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCA              >  SRR4302536.1883142/1‑94 (MQ=60)
                                                                                GACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAG             <  SRR4302536.3025184/94‑1 (MQ=60)
                                                                                  CGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTA           <  SRR4302536.4938852/94‑1 (MQ=60)
                                                                                  CGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTA           >  SRR4302536.4151401/1‑94 (MQ=60)
                                                                                     TGATGACGCTGCGCGCATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTCCACATGGCCCAGTAAAC        <  SRR4302536.72424/94‑1 (MQ=60)
                                                                                      GATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACA       >  SRR4302536.830417/1‑94 (MQ=60)
                                                                                        TGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATT     <  SRR4302536.2279104/94‑1 (MQ=60)
                                                                                        TGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATT     <  SRR4302536.4563194/94‑1 (MQ=60)
                                                                                        TGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATT     <  SRR4302536.2175898/94‑1 (MQ=60)
                                                                                           AGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAG  >  SRR4302536.4156208/1‑94 (MQ=60)
                                                                                             |                                                                                           
GCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAG  >  NC_000913/2299173‑2299357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑