Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,387,335	T→C	V68A (GTA→GCA)	b2275 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,387,335	0	T	C	99.6%	816.4 / NA	250	V68A (GTA→GCA)	b2275	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (134/115); total (134/116)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

ACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGTACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATC  >  NC_000913/2387245‑2387424

aCGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCAc                                                                                         >  1:4835546/1‑93 (MQ=255)

 cGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCAcc                                                                                        <  2:4979570/93‑1 (MQ=255)

  ggAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCt                                                                                       >  2:2564649/1‑93 (MQ=255)

  ggAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCt                                                                                       >  2:3051998/1‑93 (MQ=255)

  ggAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCt                                                                                       >  2:2072094/1‑93 (MQ=255)

    agaCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGc                                                                                     >  1:6612300/1‑93 (MQ=255)

     gaCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGcc                                                                                    <  2:2328261/93‑1 (MQ=255)

     gaCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGcc                                                                                    <  2:1953718/93‑1 (MQ=255)

      aCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCt                                                                                   <  1:5453790/93‑1 (MQ=255)

      aCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCt                                                                                   <  2:620793/93‑1 (MQ=255)

      aCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCt                                                                                   <  2:1223444/93‑1 (MQ=255)

       cTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCtt                                                                                  >  1:1300927/1‑93 (MQ=255)

       cTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCtt                                                                                  >  2:3115350/1‑93 (MQ=255)

        tGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCttt                                                                                 >  1:2286594/1‑93 (MQ=255)

           ttCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTcc                                                                              <  1:5212728/93‑1 (MQ=255)

               gTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCa                                                                          >  2:5692503/1‑93 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         >  1:2329903/1‑93 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         <  2:3787728/93‑1 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         <  2:6483988/93‑1 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGAACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         >  1:5800201/1‑93 (MQ=255)

                   gTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaa                                                                      <  1:3258178/93‑1 (MQ=255)

                    tACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaag                                                                     <  1:5514343/93‑1 (MQ=255)

                    tACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACAATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaag                                                                     >  2:4460784/1‑93 (MQ=255)

                     aCTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaaga                                                                    >  1:5591854/1‑93 (MQ=255)

                     aCTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaaga                                                                    >  2:6589620/1‑93 (MQ=255)

                      cTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaagaa                                                                   >  2:6282693/1‑93 (MQ=255)

                        ggCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGt                                                                 >  1:4348269/1‑93 (MQ=255)

                        ggCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGt                                                                 <  2:388164/93‑1 (MQ=255)

                        ggCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGt                                                                 >  2:4626481/1‑93 (MQ=255)

                         gCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGtt                                                                <  1:1772088/93‑1 (MQ=255)

                             aTTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaa                                                            <  2:6377243/93‑1 (MQ=255)

                              ttGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaag                                                           <  2:4568961/93‑1 (MQ=255)

                                ggTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaagaa                                                         <  1:5669516/93‑1 (MQ=255)

                                ggTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaagaa                                                         >  1:3897644/1‑93 (MQ=255)

                                  tCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTTGAAGAATg                                                       <  1:6547346/93‑1 (MQ=255)

                                  tCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATg                                                       <  1:6129093/93‑1 (MQ=255)

                                    aGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGc                                                     <  2:2427949/93‑1 (MQ=255)

                                    aGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGc                                                     >  1:2475061/1‑93 (MQ=255)

                                    aGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGc                                                     <  2:4750353/93‑1 (MQ=255)

                                     ggAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCt                                                    >  2:2758178/1‑93 (MQ=255)

                                     ggAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCt                                                    >  2:3391161/1‑93 (MQ=255)

                                     ggAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCt                                                    >  1:807300/1‑93 (MQ=255)

                                      gAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTg                                                   >  1:1664173/1‑93 (MQ=255)

                                      gAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTg                                                   <  1:1072987/93‑1 (MQ=255)

                                      gAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTg                                                   >  1:3439219/1‑93 (MQ=255)

                                       aGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTgg                                                  >  2:5883513/1‑93 (MQ=255)

                                        gCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGa                                                 >  1:2742233/1‑93 (MQ=255)

                                         cATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGaa                                                <  1:6235102/93‑1 (MQ=255)

                                          aTAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAg                                               <  1:2638696/93‑1 (MQ=255)

                                          aTAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTGTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGGAg                                               >  1:6146023/1‑93 (MQ=255)

                                           tAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAgg                                              >  1:5223186/1‑92 (MQ=255)

                                             aaCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGa                                            >  2:5512184/1‑93 (MQ=255)

                                             aaCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGa                                            >  1:322676/1‑93 (MQ=255)

                                             aaCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGa                                            <  2:4421638/93‑1 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATTGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAACTGaa                                           >  2:4065247/1‑93 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGaa                                           <  2:5971830/93‑1 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGaa                                           >  2:8002/1‑93 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGaa                                           <  1:3140996/93‑1 (MQ=255)

                                                tACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatg                                         >  2:6536169/1‑93 (MQ=255)

                                                tACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatg                                         <  1:4938709/93‑1 (MQ=255)

                                                 aCCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatga                                        <  1:4105942/93‑1 (MQ=255)

                                                 aCCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatga                                        >  1:3581754/1‑93 (MQ=255)

                                                  ccTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatgat                                       <  2:1652565/93‑1 (MQ=255)

                                                  ccTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatgat                                       >  2:3238801/1‑93 (MQ=255)

                                                   cTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatgatg                                      >  1:4172411/1‑93 (MQ=255)

                                                       aGTGTGCGAAACGTACCATGACGAAGGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGGAGTGAAtgaggatga                                  >  1:1351128/1‑93 (MQ=255)

                                                           tgCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTGCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATccc                              >  2:3575648/1‑93 (MQ=255)

                                                            gCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATcccc                             >  1:5120749/1‑93 (MQ=255)

                                                             cGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCa                            >  2:5088378/1‑93 (MQ=255)

                                                             cGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCa                            >  1:2884609/1‑93 (MQ=255)

                                                              gAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCAt                           >  2:5394026/1‑93 (MQ=255)

                                                                aaCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGa                         >  2:4023344/1‑93 (MQ=255)

                                                                aaCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGa                         <  2:4198122/93‑1 (MQ=255)

                                                                aaCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGa                         >  1:4467460/1‑93 (MQ=255)

                                                                 aCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGaa                        >  2:517727/1‑93 (MQ=255)

                                                                   gTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGaaaa                      <  1:6430904/93‑1 (MQ=255)

                                                                    tACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAt                     >  1:5061843/1‑93 (MQ=255)

                                                                    tACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAt                     <  2:6517984/93‑1 (MQ=255)

                                                                     aCCATGACGAATGGCGAATCGCCCCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATg                    <  1:2025673/93‑1 (MQ=255)

                                                                        aTGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCa                 >  2:2849004/1‑93 (MQ=255)

                                                                         tGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCAt                >  1:4258609/1‑93 (MQ=255)

                                                                           aCGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCa              <  2:6704595/93‑1 (MQ=255)

                                                                            cGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAg             >  2:2186345/1‑93 (MQ=255)

                                                                            cGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAg             >  2:4859275/1‑93 (MQ=255)

                                                                              aaTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCa           >  2:1741544/1‑93 (MQ=255)

                                                                              aaTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCa           <  2:3702749/93‑1 (MQ=255)

                                                                               aTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATTGCATCAGCAt          >  1:2589269/1‑93 (MQ=255)

                                                                               aTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCAt          >  1:3554305/1‑93 (MQ=255)

                                                                                    gAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAg     <  1:1038747/93‑1 (MQ=255)

                                                                                     aaTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGa    >  1:3763592/1‑93 (MQ=255)

                                                                                     aaTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGa    <  1:2156332/93‑1 (MQ=255)

                                                                                     aaTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGa    >  2:1543220/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   <  2:6010422/93‑1 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   >  2:1045993/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   >  2:4338692/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   <  2:6674031/93‑1 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   >  2:2501795/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCCAAGAt   >  2:3319982/1‑93 (MQ=255)

                                                                                       tCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATc  >  1:3353508/1‑93 (MQ=255)

                                                                                       tCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATc  <  2:2838918/93‑1 (MQ=255)

ACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGTACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATC  >  NC_000913/2387245‑2387424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTAACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGTACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGA  >  NC_000913/2387242‑2387428

GTAACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGC                                                                                               >  SRR4302536.1958254/1‑94 (MQ=60)

  AACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCAC                                                                                             >  SRR4302536.4981091/1‑94 (MQ=60)

    CGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCT                                                                                           <  SRR4302536.3287667/94‑1 (MQ=60)

    CGGAGACTGTTGCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCT                                                                                           <  SRR4302536.1582044/94‑1 (MQ=60)

     GGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTG                                                                                          >  SRR4302536.135511/1‑94 (MQ=60)

      GAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGC                                                                                         >  SRR4302536.6808914/1‑94 (MQ=60)

         ACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTT                                                                                      <  SRR4302536.5616898/94‑1 (MQ=60)

         ACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTT                                                                                      >  SRR4302536.1339799/1‑94 (MQ=60)

          CTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTT                                                                                     <  SRR4302536.5336022/94‑1 (MQ=60)

          CTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTT                                                                                     >  SRR4302536.2355939/1‑94 (MQ=60)

          CTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTT                                                                                     <  SRR4302536.3939345/94‑1 (MQ=60)

           TGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTT                                                                                    <  SRR4302536.5919645/94‑1 (MQ=60)

           TGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTT                                                                                    >  SRR4302536.2580315/1‑94 (MQ=60)

             TTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCC                                                                                  <  SRR4302536.3324994/94‑1 (MQ=60)

              TTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCA                                                                                 <  SRR4302536.3472342/94‑1 (MQ=60)

              TTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCA                                                                                 <  SRR4302536.2076683/94‑1 (MQ=60)

              TTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCA                                                                                 <  SRR4302536.5368077/94‑1 (MQ=60)

                CGGTTTGTACTGGCCTATTGGTCAGTAGCATAAGCTACCTATAGTGTGCAAAACTTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGC                                                                               <  SRR4302536.3346958/94‑1 (MQ=60)

                CGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGC                                                                               <  SRR4302536.5076133/94‑1 (MQ=60)

                CGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGC                                                                               >  SRR4302536.6780760/1‑94 (MQ=60)

                 GGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCA                                                                              <  SRR4302536.2776446/94‑1 (MQ=60)

                 GGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCA                                                                              >  SRR4302536.3559152/1‑94 (MQ=60)

                  GTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAT                                                                             >  SRR4302536.2400598/1‑94 (MQ=60)

                  GTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGAACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAT                                                                             >  SRR4302536.5972903/1‑94 (MQ=60)

                   TTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATT                                                                            <  SRR4302536.6318620/94‑1 (MQ=60)

                      GTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAG                                                                         <  SRR4302536.4100213/94‑1 (MQ=60)

                      GTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAG                                                                         <  SRR4302536.3353680/94‑1 (MQ=60)

                       TACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGA                                                                        <  SRR4302536.5678805/94‑1 (MQ=60)

                       TACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGA                                                                        >  SRR4302536.5758134/1‑94 (MQ=60)

                        ACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAA                                                                       <  SRR4302536.3792278/94‑1 (MQ=60)

                        ACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGCACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAA                                                                       >  SRR4302536.2931106/1‑94 (MQ=60)

                          TGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGT                                                                     <  SRR4302536.4525603/94‑1 (MQ=60)

                          TGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGT                                                                     >  SRR4302536.4478405/1‑94 (MQ=60)

                           GGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTT                                                                    >  SRR4302536.2472458/1‑94 (MQ=60)

                            GCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTC                                                                   >  SRR4302536.2457511/1‑94 (MQ=60)

                            GCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTC                                                                   <  SRR4302536.1825692/94‑1 (MQ=60)

                             cttcTTGGTCAGGAGCATAAACTACCTGTAGTGTGCGAAACGTACCGTGACGGATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGATCG                                                                  >  SRR4302536.6300349/5‑94 (MQ=60)

                              CTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGA                                                                 >  SRR4302536.4819110/1‑94 (MQ=60)

                               TATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAA                                                                >  SRR4302536.6906623/1‑94 (MQ=60)

                                ATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAG                                                               <  SRR4302536.2274283/94‑1 (MQ=60)

                                ATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAG                                                               <  SRR4302536.3222250/94‑1 (MQ=60)

                                ATTGGTCAGCAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAG                                                               <  SRR4302536.179290/94‑1 (MQ=60)

                                 TTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGA                                                              <  SRR4302536.2070328/94‑1 (MQ=60)

                                  TGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAA                                                             >  SRR4302536.4013528/1‑94 (MQ=60)

                                  TGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAA                                                             <  SRR4302536.4224831/94‑1 (MQ=60)

                                   GGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAAT                                                            <  SRR4302536.5838042/94‑1 (MQ=60)

                                   GGTCAGGAGCATAAACTACCTATAGTGTCCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAAT                                                            <  SRR4302536.4571195/94‑1 (MQ=60)

                                    GTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATG                                                           <  SRR4302536.1258838/94‑1 (MQ=60)

                                     TCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTTGAAGAATGG                                                          <  SRR4302536.6742312/94‑1 (MQ=60)

                                     TCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGG                                                          <  SRR4302536.6311768/94‑1 (MQ=60)

                                      CAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGC                                                         >  SRR4302536.2549777/1‑94 (MQ=60)

                                       AGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCT                                                        >  SRR4302536.6001998/1‑94 (MQ=60)

                                       AGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCT                                                        <  SRR4302536.5407180/94‑1 (MQ=60)

                                       AGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCT                                                        >  SRR4302536.6173409/1‑94 (MQ=60)

                                       AGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCT                                                        >  SRR4302536.830971/1‑94 (MQ=60)

                                       AGGAGCATAAACTACCTATAGTGTGCGAAACGCACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCT                                                        >  SRR4302536.2705436/1‑94 (MQ=60)

                                        GGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTG                                                       >  SRR4302536.3541642/1‑94 (MQ=60)

                                        GGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTG                                                       <  SRR4302536.3632281/94‑1 (MQ=60)

                                        GGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTG                                                       >  SRR4302536.1712461/1‑94 (MQ=60)

                                         GAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGG                                                      <  SRR4302536.1105114/94‑1 (MQ=60)

                                         GAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGG                                                      <  SRR4302536.5753599/94‑1 (MQ=60)

                                          AGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGA                                                     >  SRR4302536.2823924/1‑94 (MQ=60)

                                            CATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAG                                                   <  SRR4302536.6420861/94‑1 (MQ=60)

                                            CATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTGTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGGAG                                                   >  SRR4302536.6329132/1‑94 (MQ=60)

                                             ATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGT                                                  <  SRR4302536.2717806/94‑1 (MQ=60)

                                             ATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGG                                                  >  SRR4302536.5378818/1‑94 (MQ=60)

                                              TAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTG                                                 <  SRR4302536.5297191/94‑1 (MQ=60)

                                              TAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTG                                                 <  SRR4302536.3671676/94‑1 (MQ=60)

                                              TAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTG                                                 <  SRR4302536.4277593/94‑1 (MQ=60)

                                               AAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGA                                                >  SRR4302536.331233/1‑94 (MQ=60)

                                                AACTACCTATAGTGTGCGAATCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAA                                               >  SRR4302536.6740383/1‑94 (MQ=60)

                                                 ACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAAT                                              <  SRR4302536.3232953/94‑1 (MQ=60)

                                                  CTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATG                                             >  SRR4302536.2395162/1‑94 (MQ=60)

                                                  CTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATG                                             >  SRR4302536.5709487/1‑94 (MQ=60)

                                                   TACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGA                                            <  SRR4302536.5087123/94‑1 (MQ=60)

                                                   TACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGA                                            >  SRR4302536.3688854/1‑94 (MQ=60)

                                                    ACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGAT                                           <  SRR4302536.4227756/94‑1 (MQ=60)

                                                    ACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGAT                                           <  SRR4302536.2994113/94‑1 (MQ=60)

                                                     CCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATG                                          <  SRR4302536.5620436/94‑1 (MQ=60)

                                                     CCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATG                                          >  SRR4302536.4296355/1‑94 (MQ=60)

                                                        ATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATG                                       >  SRR4302536.5175755/1‑94 (MQ=60)

                                                         TAGTGTGCGAAACGTACCATGACGAAGGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGGAGTGAATGAGGATGA                                      >  SRR4302536.1391419/1‑94 (MQ=60)

                                                          AGTATGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGAT                                     >  SRR4302536.5762615/1‑94 (MQ=60)

                                                              TGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCC                                 >  SRR4302536.5273750/1‑94 (MQ=60)

                                                               GCGAAGCGTACCATGACGAAGGGCGAATCGCACCTGCTTTTTCCAGCATTAAGAGGTGCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCA                                <  SRR4302536.2077379/94‑1 (MQ=60)

                                                               GCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCA                                >  SRR4302536.2969723/1‑94 (MQ=60)

                                                               GCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCA                                <  SRR4302536.2649942/94‑1 (MQ=60)

                                                                CGAACCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGATCGAAGAATGGCTGGAAGagattgatatggacccccag                               >  SRR4302536.3103891/1‑74 (MQ=60)

                                                                CGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCAT                               >  SRR4302536.2295209/1‑94 (MQ=60)

                                                                CGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATAATCCCCAT                               >  SRR4302536.5059108/1‑94 (MQ=60)

                                                                  AAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGA                             >  SRR4302536.4601663/1‑94 (MQ=60)

                                                                    ACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAA                           >  SRR4302536.4359529/1‑94 (MQ=60)

                                                                     CGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAA                          >  SRR4302536.666605/1‑94 (MQ=60)

                                                                     CGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAA                          >  SRR4302536.6457100/1‑94 (MQ=60)

                                                                      GTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAT                         <  SRR4302536.6623112/94‑1 (MQ=60)

                                                                      GTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAT                         >  SRR4302536.5213468/1‑94 (MQ=60)

                                                                      GTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAT                         >  SRR4302536.5457631/1‑94 (MQ=60)

                                                                      GTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAT                         <  SRR4302536.219070/94‑1 (MQ=60)

                                                                       TACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTTAATGATGATGATCCCCATGAAAATG                        >  SRR4302536.5177755/1‑94 (MQ=60)

                                                                        ACCATGACGAATGGCGAATCGCCCCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGG                       <  SRR4302536.2086684/94‑1 (MQ=60)

                                                                        ACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGG                       >  SRR4302536.2804142/1‑94 (MQ=60)

                                                                         CCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGC                      >  SRR4302536.836728/1‑94 (MQ=60)

                                                                          CATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCA                     >  SRR4302536.3756343/1‑94 (MQ=60)

                                                                          CATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCA                     >  SRR4302536.1177092/1‑94 (MQ=60)

                                                                           ATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCAT                    >  SRR4302536.4385496/1‑94 (MQ=60)

                                                                           ATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCAT                    <  SRR4302536.3480110/94‑1 (MQ=60)

                                                                              ACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAG                 >  SRR4302536.5845590/1‑94 (MQ=60)

                                                                              ACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAG                 >  SRR4302536.1556392/1‑94 (MQ=60)

                                                                              ACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGACCCCCATGAAAATGGCATCAG                 <  SRR4302536.3370/94‑1 (MQ=60)

                                                                                 AATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATTGCATCAGCAT              >  SRR4302536.2667037/1‑94 (MQ=60)

                                                                                 AATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCAT              <  SRR4302536.5241789/94‑1 (MQ=60)

                                                                                 AATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCAT              >  SRR4302536.3660574/1‑94 (MQ=60)

                                                                                  ATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATC             <  SRR4302536.4361530/94‑1 (MQ=60)

                                                                                   TGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCG            >  SRR4302536.773380/1‑94 (MQ=60)

                                                                                   TGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCG            >  SRR4302536.1086922/1‑94 (MQ=60)

                                                                                       GAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGA        >  SRR4302536.6339735/1‑94 (MQ=60)

                                                                                       GAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGA        >  SRR4302536.6376562/1‑94 (MQ=60)

                                                                                       GAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGA        >  SRR4302536.5374536/1‑94 (MQ=60)

                                                                                       GAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGA        >  SRR4302536.3875992/1‑94 (MQ=60)

                                                                                       GAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGA        <  SRR4302536.1069590/94‑1 (MQ=60)

                                                                                        AATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAT       >  SRR4302536.4425515/1‑94 (MQ=60)

                                                                                        AATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAT       <  SRR4302536.2221160/94‑1 (MQ=60)

                                                                                         ATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATC      >  SRR4302536.3453019/1‑94 (MQ=60)

                                                                                            GCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAG   <  SRR4302536.3515286/94‑1 (MQ=60)

                                                                                            GCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAG   <  SRR4302536.1873322/94‑1 (MQ=60)

                                                                                             CACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAGAATGGCATCAGCATCGAAGATCAAGA  >  SRR4302536.1803142/1‑94 (MQ=60)

                                                                                             CACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGA  >  SRR4302536.5937310/1‑94 (MQ=60)

                                                                                             CACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGA  >  SRR4302536.3515377/1‑94 (MQ=60)

                                                                                             CACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGA  >  SRR4302536.2432118/1‑94 (MQ=60)

                                                                                             CACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGA  >  SRR4302536.1119611/1‑94 (MQ=60)

                                                                                             CACCTGCCTTTTCCAGCATTAAGAAGCTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGA  <  SRR4302536.5554024/94‑1 (MQ=60)

GTAACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGTACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGA  >  NC_000913/2387242‑2387428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`