Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,409,738	T→C	A130A (GCA→GCG)	yfbT ←	putative phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,409,738	0	T	C	99.5%	684.4 / NA	211	A130A (GCA→GCG)	yfbT	putative phosphatase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (99/111); minor base A (1/0); total (100/111)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

CGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAA  >  NC_000913/2409648‑2409827

   aaGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCggg                                                                                      >  2:1960369/1‑93 (MQ=255)

    aGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGa                                                                                     >  1:950803/1‑93 (MQ=255)

        ccAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATcc                                                                                 <  1:4931454/93‑1 (MQ=255)

        ccAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGcc                                                                                 <  1:6453436/93‑1 (MQ=255)

          agcagcTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCa                                                                               <  2:4646485/93‑1 (MQ=255)

           gcagcTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAg                                                                              >  2:2484284/1‑93 (MQ=255)

             agcTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCt                                                                            >  1:1435513/1‑93 (MQ=255)

              gcTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTa                                                                           <  1:4089673/93‑1 (MQ=255)

               cTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTAt                                                                          <  1:173817/93‑1 (MQ=255)

                 gcgcgcCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTAttt                                                                        <  1:3226072/93‑1 (MQ=255)

                  cgcgcCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATCCCAGCTAtttt                                                                       <  1:5499514/93‑1 (MQ=255)

                     gcCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATg                                                                    >  1:5231900/1‑93 (MQ=255)

                      ccTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATgg                                                                   <  1:2489084/93‑1 (MQ=255)

                       cTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgc                                                                  >  2:1818501/1‑93 (MQ=255)

                        tAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcg                                                                 <  1:5668221/93‑1 (MQ=255)

                        tAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcg                                                                 >  1:5295819/1‑93 (MQ=255)

                        tAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcg                                                                 >  1:5132747/1‑93 (MQ=255)

                        tAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcg                                                                 >  2:2263139/1‑93 (MQ=255)

                        tAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcg                                                                 <  1:3783746/93‑1 (MQ=255)

                        tAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcg                                                                 >  1:1851399/1‑93 (MQ=255)

                        tAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcg                                                                 >  1:2246886/1‑93 (MQ=255)

                         aaCAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcgc                                                                >  1:3186260/1‑93 (MQ=255)

                          aCAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcgcg                                                               >  2:2712337/1‑93 (MQ=255)

                           cAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGgcgcgc                                                              <  1:162815/93‑1 (MQ=255)

                            aGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGcgcgct                                                             <  2:4035634/93‑1 (MQ=255)

                             gATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGcgcgctc                                                            >  1:5044612/1‑93 (MQ=255)

                             gATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGcgcgctc                                                            >  1:1324380/1‑93 (MQ=255)

                             gATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGcgcgctc                                                            <  2:1365381/93‑1 (MQ=255)

                             gATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGcgcgctc                                                            <  1:3004615/93‑1 (MQ=255)

                                aCGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGcgcgctcgcg                                                         >  1:3318302/1‑93 (MQ=255)

                                  gCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGcgcgctcgcgct                                                       >  1:573271/1‑93 (MQ=255)

                                    aTCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTAc                                                     >  2:2626746/1‑93 (MQ=255)

                                    aTCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTAc                                                     <  1:6234547/93‑1 (MQ=255)

                                    aTCAGGTTCTGGTATTCCACGCATCACTCGCTCAGCGGTTACAAACGCCTAAGGCGCGGGAAGCCCAGATAATTAATGGCGCGCTCGCGCTAc                                                     <  1:6720940/93‑1 (MQ=255)

                                     tCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTAcc                                                    >  1:3719606/1‑93 (MQ=255)

                                     tCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTAcc                                                    >  2:4748168/1‑93 (MQ=255)

                                      cAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTGACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCg                                                   >  2:5487473/1‑93 (MQ=255)

                                       aGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCgg                                                  <  2:5685234/93‑1 (MQ=255)

                                       aGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCgg                                                  <  2:6626730/93‑1 (MQ=255)

                                       aGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCgg                                                  >  2:3683298/1‑93 (MQ=255)

                                        ggTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGc                                                 >  1:3955769/1‑93 (MQ=255)

                                        ggTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGc                                                 <  2:5078273/93‑1 (MQ=255)

                                          ttCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGCAGCCCAGCTATTTTATGGCGCACTCGCGCTACCGGCAt                                               >  1:5031246/1‑93 (MQ=255)

                                          ttCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCAt                                               <  2:1574909/93‑1 (MQ=255)

                                          gtCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCAt                                               >  2:6316445/2‑93 (MQ=255)

                                           tCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATg                                              <  2:5333143/93‑1 (MQ=255)

                                            cTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATgg                                             <  2:3994144/93‑1 (MQ=255)

                                            cTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATgg                                             <  2:5995528/93‑1 (MQ=255)

                                               gctttCCGCGCTTCACTCGCTCAGCGGTTACAACCACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGc                                          <  1:4734090/91‑1 (MQ=255)

                                               gTTTTCCGCGCTTCACTCGCTCCGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGc                                          >  1:3818795/1‑93 (MQ=255)

                                                ttttCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGcc                                         <  2:3359630/93‑1 (MQ=255)

                                                ttttCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGcc                                         >  1:4562828/1‑93 (MQ=255)

                                                   tCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGa                                      <  2:4477293/93‑1 (MQ=255)

                                                   tCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGa                                      <  1:1935976/93‑1 (MQ=255)

                                                     cgcgCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAg                                    >  1:2473366/1‑93 (MQ=255)

                                                     cgcgCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAg                                    <  1:2658105/93‑1 (MQ=255)

                                                      gcgcTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGt                                   <  2:782647/93‑1 (MQ=255)

                                                       cgcTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTc                                  <  2:3274469/93‑1 (MQ=255)

                                                        gcTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTca                                 >  1:4361075/1‑93 (MQ=255)

                                                         cTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATAGAGCCAGAAGTcac                                >  2:319788/1‑93 (MQ=255)

                                                          ttCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTcaca                               <  2:5506451/93‑1 (MQ=255)

                                                          ttCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTcaca                               >  2:2106712/1‑93 (MQ=255)

                                                           tCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACaa                              <  1:2023478/93‑1 (MQ=255)

                                                           tCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACaa                              >  2:1605369/1‑93 (MQ=255)

                                                             aCTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATg                            <  1:2722679/93‑1 (MQ=255)

                                                             aCTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATg                            >  1:6003038/1‑93 (MQ=255)

                                                             aCTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATg                            >  1:2343794/1‑93 (MQ=255)

                                                              cTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATgg                           >  2:5843035/1‑93 (MQ=255)

                                                              cTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATgg                           <  1:1040253/93‑1 (MQ=255)

                                                               tCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGc                          <  1:299627/93‑1 (MQ=255)

                                                                 gCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGccc                        >  1:726804/1‑93 (MQ=255)

                                                                   tCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCAc                      <  1:2283730/93‑1 (MQ=255)

                                                                   tCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACAGGCATGGAGCCAGAAGTCACAATGGCCCAc                      >  2:337954/1‑93 (MQ=255)

                                                                     aGCGGTTACAAACACCTCTGGAGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACgg                    >  1:5566321/1‑93 (MQ=255)

                                                                       cGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGaa                  >  2:6699647/1‑93 (MQ=255)

                                                                        ggTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAAt                 <  1:2125815/93‑1 (MQ=255)

                                                                        ggTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAAt                 >  2:3578581/1‑93 (MQ=255)

                                                                        ggTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAAt                 >  1:637880/1‑93 (MQ=255)

                                                                        ggTCACAAACACCTCTGGCGCGGGAAACCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAAt                 <  1:1218904/93‑1 (MQ=255)

                                                                         gTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATa                <  2:5440098/93‑1 (MQ=255)

                                                                           tACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATAcc              <  2:3236181/93‑1 (MQ=255)

                                                                            aCAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCt             <  2:6476740/93‑1 (MQ=255)

                                                                            aCAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCt             <  1:4883644/93‑1 (MQ=255)

                                                                            aCAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCt             >  1:872159/1‑93 (MQ=255)

                                                                             cAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTg            >  2:1984041/1‑93 (MQ=255)

                                                                              aaaCACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGc           >  2:4389055/1‑93 (MQ=255)

                                                                              aaaCACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGc           <  2:4922635/93‑1 (MQ=255)

                                                                               aaCACCTCTGGCGCGGGAATCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCt          <  2:923853/93‑1 (MQ=255)

                                                                               aaCACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCt          <  1:3376977/93‑1 (MQ=255)

                                                                                acacCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCtt         >  2:5559053/1‑93 (MQ=255)

                                                                                 cacGTCTGGCGCGGGACTCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCttt        <  1:5753508/93‑1 (MQ=255)

                                                                                 cacCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCttt        <  1:2806105/93‑1 (MQ=255)

                                                                                  acCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTa       >  1:6139759/1‑93 (MQ=255)

                                                                                    ctctGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTAtt     <  2:4260328/93‑1 (MQ=255)

                                                                                     tctGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTc    >  2:1116278/1‑93 (MQ=255)

                                                                                     tctGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTc    <  2:2804756/93‑1 (MQ=255)

                                                                                     tctGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTc    >  2:6420839/1‑93 (MQ=255)

                                                                                     tctGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTc    >  2:330815/1‑93 (MQ=255)

                                                                                      ctGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCa   <  1:3564604/93‑1 (MQ=255)

                                                                                       tGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCaa  <  2:6186533/93‑1 (MQ=255)

CGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAA  >  NC_000913/2409648‑2409827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATGA  >  NC_000913/2409645‑2409831

CGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGC                                                                                               <  SRR4302536.130707/94‑1 (MQ=60)

  GCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGC                                                                                             <  SRR4302536.1397257/94‑1 (MQ=60)

     CAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGG                                                                                          <  SRR4302536.2053914/94‑1 (MQ=60)

      AAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGA                                                                                         >  SRR4302536.978902/1‑94 (MQ=60)

        GCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAG                                                                                       <  SRR4302536.3553630/94‑1 (MQ=60)

         CCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGC                                                                                      >  SRR4302536.6373569/1‑94 (MQ=60)

         CCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGC                                                                                      >  SRR4302536.2510921/1‑94 (MQ=60)

          CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGGTTTCCGCGCTTCACTCGGTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCC                                                                                     >  SRR4302536.2259598/1‑94 (MQ=60)

           CCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATCCC                                                                                    <  SRR4302536.5079624/94‑1 (MQ=60)

           CCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCC                                                                                    <  SRR4302536.6646173/94‑1 (MQ=60)

            CAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCA                                                                                   >  SRR4302536.5389497/1‑94 (MQ=60)

             AGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAG                                                                                  <  SRR4302536.3415499/94‑1 (MQ=60)

             AGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACATCTGGCGCGGGAAGCCCAG                                                                                  >  SRR4302536.2730506/1‑94 (MQ=60)

               CAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCT                                                                                <  SRR4302536.3241602/94‑1 (MQ=60)

               CAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCT                                                                                >  SRR4302536.1478104/1‑94 (MQ=60)

                 GCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTAT                                                                              <  SRR4302536.4210920/94‑1 (MQ=60)

                  accgctgagcgagtGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATT                                                                             >  SRR4302536.3405096/15‑94 (MQ=60)

                  CTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATT                                                                             <  SRR4302536.178363/94‑1 (MQ=60)

                    GCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTT                                                                           <  SRR4302536.3320651/94‑1 (MQ=60)

                     CGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATCCCAGCTATTTTA                                                                          <  SRR4302536.5663599/94‑1 (MQ=60)

                      GCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTAT                                                                         <  SRR4302536.5560322/94‑1 (MQ=60)

                       CGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATG                                                                        >  SRR4302536.5387786/1‑94 (MQ=60)

                         CCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATTGC                                                                      >  SRR4302536.505980/1‑94 (MQ=60)

                         CCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGC                                                                      <  SRR4302536.2564177/94‑1 (MQ=60)

                          CTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCG                                                                     >  SRR4302536.5286034/1‑94 (MQ=60)

                          CTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCG                                                                     >  SRR4302536.2314900/1‑94 (MQ=60)

                          CTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCG                                                                     <  SRR4302536.1697390/94‑1 (MQ=60)

                          CTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCG                                                                     >  SRR4302536.1907199/1‑94 (MQ=60)

                          CTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCG                                                                     >  SRR4302536.5453741/1‑94 (MQ=60)

                           TAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGC                                                                    <  SRR4302536.5836705/94‑1 (MQ=60)

                           TAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGC                                                                    >  SRR4302536.3279678/1‑94 (MQ=60)

                           TAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGC                                                                    <  SRR4302536.3896642/94‑1 (MQ=60)

                            AACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCG                                                                   <  SRR4302536.531359/94‑1 (MQ=60)

                             ACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGC                                                                  >  SRR4302536.908723/1‑94 (MQ=60)

                              CAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCT                                                                 <  SRR4302536.167039/94‑1 (MQ=60)

                              CAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCT                                                                 <  SRR4302536.1791688/94‑1 (MQ=60)

                              CAGATACGCATCAGGGTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGTGCGCG                                                                 >  SRR4302536.5982406/1‑94 (MQ=60)

                               AGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTC                                                                <  SRR4302536.3906970/94‑1 (MQ=60)

                               AGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTC                                                                >  SRR4302536.5195791/1‑94 (MQ=60)

                               AGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTC                                                                >  SRR4302536.1363913/1‑94 (MQ=60)

                                GATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCG                                                               <  SRR4302536.4093531/94‑1 (MQ=60)

                                GATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCG                                                               <  SRR4302536.3092967/94‑1 (MQ=60)

                                 ATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGC                                                              >  SRR4302536.5736328/1‑94 (MQ=60)

                                  TACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCG                                                             >  SRR4302536.3416389/1‑94 (MQ=60)

                                   ACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGC                                                            <  SRR4302536.2678563/94‑1 (MQ=60)

                                    CGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCT                                                           >  SRR4302536.587841/1‑94 (MQ=60)

                                     ccacCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTA                                                          <  SRR4302536.5915894/90‑1 (MQ=60)

                                     GCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTA                                                          >  SRR4302536.2362336/1‑94 (MQ=60)

                                      CATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTAC                                                         >  SRR4302536.872993/1‑94 (MQ=60)

                                      CATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTAC                                                         <  SRR4302536.1295910/94‑1 (MQ=60)

                                      CATCAGGTTCTGGTTTTCCGCGCGTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGATGCCCAGCTATTTTATGGCGCGCTCGCGCTAC                                                         <  SRR4302536.2347699/94‑1 (MQ=60)

                                       ATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACC                                                        >  SRR4302536.1232437/1‑94 (MQ=60)

                                       ATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACC                                                        >  SRR4302536.3830794/1‑94 (MQ=60)

                                       ATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACC                                                        <  SRR4302536.6420293/94‑1 (MQ=60)

                                       ATCAGGTTCTGGTATTCCACGCATCACTCGCTCAGCGGTTACAAACGCCTAAGGCGCGGGAAGCCCAGATAATTAATGGCGCGCTCGCGCTACC                                                        <  SRR4302536.6920514/94‑1 (MQ=42)

                                        TCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCG                                                       >  SRR4302536.5672300/1‑94 (MQ=60)

                                        TCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCG                                                       <  SRR4302536.3689040/94‑1 (MQ=60)

                                          AGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGC                                                     <  SRR4302536.383722/94‑1 (MQ=60)

                                          AGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGC                                                     >  SRR4302536.4073231/1‑94 (MQ=60)

                                           GGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCA                                                    >  SRR4302536.4891866/1‑94 (MQ=60)

                                            GTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGCAGCCCAGCTATTTTATGGCGCACTCGCGCTACCGGCAT                                                   >  SRR4302536.5182075/1‑94 (MQ=60)

                                                TGGTTTTCCGCTCTTCACTCGCTCAGCGGTTACAAACGCCTCTGGCGCGGGAACCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAG                                               <  SRR4302536.4456644/94‑1 (MQ=60)

                                                 GGTTTTCCGCGCTTCACTCGCTCCGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGC                                              >  SRR4302536.3932603/1‑94 (MQ=60)

                                                 GGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGC                                              >  SRR4302536.3066148/1‑94 (MQ=60)

                                                  GTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCC                                             >  SRR4302536.4699725/1‑94 (MQ=60)

                                                  GCTTTCCGCGCTTCACTCGCTCAGCGGTTACAACCACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCC                                             <  SRR4302536.4876561/94‑1 (MQ=60)

                                                   TTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCA                                            <  SRR4302536.3261328/94‑1 (MQ=60)

                                                   TTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCA                                            <  SRR4302536.6535944/94‑1 (MQ=60)

                                                    TTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAG                                           <  SRR4302536.5135041/94‑1 (MQ=60)

                                                      TCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAA                                         <  SRR4302536.1994204/94‑1 (MQ=60)

                                                       CCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAG                                        >  SRR4302536.2548035/1‑94 (MQ=60)

                                                        CGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGT                                       >  SRR4302536.5896427/1‑94 (MQ=60)

                                                        CGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGT                                       <  SRR4302536.2737673/94‑1 (MQ=60)

                                                        CGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGAGCTACCGGCATGGAGCCAGAAGC                                       >  SRR4302536.4045613/1‑94 (MQ=60)

                                                         GCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTC                                      >  SRR4302536.3687259/1‑94 (MQ=60)

                                                          CGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCA                                     >  SRR4302536.2563904/1‑94 (MQ=60)

                                                          CGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCA                                     >  SRR4302536.2775667/1‑94 (MQ=60)

                                                          CGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCA                                     >  SRR4302536.4491441/1‑94 (MQ=60)

                                                            CTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGCAGTCACA                                   >  SRR4302536.1512598/1‑94 (MQ=60)

                                                            CTTCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACA                                   <  SRR4302536.6289502/94‑1 (MQ=60)

                                                              TCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAATCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAAT                                 <  SRR4302536.2084413/94‑1 (MQ=60)

                                                              TCACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAAT                                 <  SRR4302536.741584/94‑1 (MQ=60)

                                                               CTCTCGCTGAGCGCTTACAAACACCTCTGGCGCGGGAATCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATG                                <  SRR4302536.4846581/94‑1 (MQ=60)

                                                               CACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATG                                >  SRR4302536.6182002/1‑94 (MQ=60)

                                                               CACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATG                                >  SRR4302536.2414879/1‑94 (MQ=60)

                                                               CACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATG                                >  SRR4302536.5194535/1‑94 (MQ=60)

                                                                ACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGG                               <  SRR4302536.2803873/94‑1 (MQ=60)

                                                                ACTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGG                               <  SRR4302536.4229670/94‑1 (MQ=60)

                                                                 CTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAGTCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGC                              <  SRR4302536.3463902/94‑1 (MQ=60)

                                                                 CTCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGC                              <  SRR4302536.1071161/94‑1 (MQ=60)

                                                                  TCGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCC                             <  SRR4302536.307585/94‑1 (MQ=60)

                                                                   CGCTCAGCGGTTACAAACGCCTCTGGCGCGGGAGTCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCC                            <  SRR4302536.209835/94‑1 (MQ=60)

                                                                   CGCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCC                            >  SRR4302536.748081/1‑94 (MQ=60)

                                                                    GCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGATCCAGAAGTCATAATGGCCCA                           <  SRR4302536.6702882/94‑1 (MQ=60)

                                                                    GCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCA                           <  SRR4302536.3087979/94‑1 (MQ=60)

                                                                    GCTCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCA                           >  SRR4302536.225108/1‑94 (MQ=60)

                                                                      TCAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACG                         <  SRR4302536.2352979/94‑1 (MQ=60)

                                                                       CAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGG                        <  SRR4302536.6900030/94‑1 (MQ=60)

                                                                       CAGCGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGG                        <  SRR4302536.1988632/94‑1 (MQ=60)

                                                                       CAGCGGTTACAAACACCTCTGGAGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGG                        >  SRR4302536.5732059/1‑94 (MQ=60)

                                                                          ccgcTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAAT                     <  SRR4302536.2819153/90‑1 (MQ=60)

                                                                          CGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAAT                     >  SRR4302536.4595019/1‑94 (MQ=60)

                                                                          CGGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAAT                     >  SRR4302536.656722/1‑94 (MQ=60)

                                                                           GGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATA                    <  SRR4302536.2189661/94‑1 (MQ=60)

                                                                           GGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATA                    >  SRR4302536.6159179/1‑94 (MQ=60)

                                                                           GGTTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGACCACGGAATA                    >  SRR4302536.5281305/1‑94 (MQ=60)

                                                                           GGTCACAAACACCTCTGGCGCGGGAAACCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATA                    <  SRR4302536.1255509/94‑1 (MQ=60)

                                                                            GTTACACACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATAC                   <  SRR4302536.5050559/94‑1 (MQ=60)

                                                                             TTACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACC                  >  SRR4302536.6615477/1‑94 (MQ=60)

                                                                              TACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCT                 >  SRR4302536.897854/1‑94 (MQ=60)

                                                                               ACAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTG                <  SRR4302536.5030554/94‑1 (MQ=60)

                                                                                CAAACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGC               <  SRR4302536.3312952/94‑1 (MQ=60)

                                                                                 AAGCACCTCTGGCGCGGGAATCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCT              <  SRR4302536.5578225/94‑1 (MQ=60)

                                                                                  AACACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTT             <  SRR4302536.3477316/94‑1 (MQ=60)

                                                                                   ACACTTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTT            <  SRR4302536.1460806/94‑1 (MQ=60)

                                                                                    CACGTCTGGCGCGGGACTCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTA           <  SRR4302536.5924645/94‑1 (MQ=60)

                                                                                    CACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTA           >  SRR4302536.6322726/1‑94 (MQ=60)

                                                                                    CACCTCTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTA           <  SRR4302536.2889317/94‑1 (MQ=60)

                                                                                        TCTGTCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCA       <  SRR4302536.3253706/94‑1 (MQ=60)

                                                                                         CTGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAA      <  SRR4302536.3671194/94‑1 (MQ=60)

                                                                                          TGGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAA     <  SRR4302536.1750482/94‑1 (MQ=60)

                                                                                           GGCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAAT    <  SRR4302536.404279/94‑1 (MQ=60)

                                                                                            GCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATG   <  SRR4302536.645343/94‑1 (MQ=60)

                                                                                            GCGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATG   <  SRR4302536.4500779/94‑1 (MQ=60)

                                                                                             CGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATGA  >  SRR4302536.608804/1‑94 (MQ=60)

                                                                                             CGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATGA  <  SRR4302536.3815139/94‑1 (MQ=60)

CGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCCCAGCTATTTTATGGCGCGCTCGCGCTACCGGCATGGAGCCAGAAGTCACAATGGCCCACGGAATACCTGCTTTATTCAAATGA  >  NC_000913/2409645‑2409831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`