Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,448,171	T→C	N147S (AAC→AGC)	b2333 ←	putative fimbrial‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,448,171	0	T	C	99.6%	755.5 / NA	232	N147S (AAC→AGC)	b2333	putative fimbrial‑like protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (116/115); minor base G (0/1); total (116/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

TCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACT  >  NC_000913/2448081‑2448260

tCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTa                                                                                         >  2:1495037/1‑93 (MQ=255)

tCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTa                                                                                         >  1:2386271/1‑93 (MQ=255)

 cAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTAt                                                                                        <  2:3088069/93‑1 (MQ=255)

 cAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTAt                                                                                        <  1:5635248/93‑1 (MQ=255)

  aaaTGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTAtt                                                                                       <  1:5718555/93‑1 (MQ=255)

   aaTGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTg                                                                                      >  2:4354521/1‑93 (MQ=255)

    aTGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGc                                                                                     >  2:6221465/1‑93 (MQ=255)

     tGTCGCAGTTAATGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGcc                                                                                    >  1:5344451/1‑93 (MQ=255)

     tGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGcc                                                                                    <  1:6603474/93‑1 (MQ=255)

      gTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCa                                                                                   <  2:4246810/93‑1 (MQ=255)

         gCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTg                                                                                <  2:5022249/93‑1 (MQ=255)

         gCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCCTTTCGCCTGTAGCCACGCTCTGAAATTGATATCTCTATTGCCATTg                                                                                >  1:5375265/1‑93 (MQ=255)

          cAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTgg                                                                               <  1:2559600/93‑1 (MQ=255)

            gTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGtt                                                                             <  2:3201275/93‑1 (MQ=255)

             ttAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTa                                                                            <  2:2651121/93‑1 (MQ=255)

               aaaGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAgc                                                                          >  2:4847301/1‑93 (MQ=255)

                aaGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAgcg                                                                         >  1:1340468/1‑93 (MQ=255)

                aaGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAgcg                                                                         >  2:743433/1‑93 (MQ=255)

                  gagGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCa                                                                       >  2:6453285/1‑93 (MQ=255)

                  gagGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCa                                                                       >  1:1457761/1‑93 (MQ=255)

                   agGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCaa                                                                      >  1:6549695/1‑93 (MQ=255)

                   agGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCaa                                                                      >  1:4715368/1‑93 (MQ=255)

                      cGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAgg                                                                   >  1:831932/1‑93 (MQ=255)

                      cGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAgg                                                                   <  1:458480/93‑1 (MQ=255)

                       ggTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGt                                                                  <  1:2691987/93‑1 (MQ=255)

                       ggTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGt                                                                  >  2:3938821/1‑93 (MQ=255)

                       ggTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGt                                                                  <  1:4817296/93‑1 (MQ=255)

                        gTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTc                                                                 >  2:2906420/1‑93 (MQ=255)

                        gTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTc                                                                 >  2:1218088/1‑93 (MQ=255)

                        gTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTc                                                                 <  1:2322423/93‑1 (MQ=255)

                        gTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTc                                                                 >  1:4887914/1‑93 (MQ=255)

                          aaaTTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGc                                                               >  1:362062/1‑93 (MQ=255)

                            aTTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGACGccc                                                             >  2:4576920/1‑93 (MQ=255)

                               cACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAt                                                          <  1:6441429/93‑1 (MQ=255)

                                agcGATCGTCACATCACTGCCGCTTTTCGGCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAtt                                                         <  1:3357558/91‑1 (MQ=255)

                                aCCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAtt                                                         <  1:2984461/93‑1 (MQ=255)

                                aCCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAtt                                                         >  2:3880554/1‑93 (MQ=255)

                                aCCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAtt                                                         <  1:349945/93‑1 (MQ=255)

                                aCCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAtt                                                         <  1:3946757/93‑1 (MQ=255)

                                 ccGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAttt                                                        >  1:748197/1‑93 (MQ=255)

                                 ccGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAttt                                                        >  1:4056078/1‑93 (MQ=255)

                                 ccGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAttt                                                        <  2:5472398/93‑1 (MQ=255)

                                  cGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTg                                                       <  2:1151887/93‑1 (MQ=255)

                                  cGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTg                                                       <  1:1096270/93‑1 (MQ=255)

                                  cGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCAATTGGTTAGCGCAAAGGTCGCCCCATTTg                                                       >  2:1360095/1‑93 (MQ=255)

                                    aTCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTa                                                     >  1:2060191/1‑93 (MQ=255)

                                     tCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAt                                                    <  1:5987808/93‑1 (MQ=255)

                                     tCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAt                                                    <  1:5673243/93‑1 (MQ=255)

                                      cGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCACAGGTCGCCCCATTTGTAtt                                                   >  2:181820/1‑93 (MQ=255)

                                      cGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAtt                                                   <  2:3036684/93‑1 (MQ=255)

                                      cGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAtt                                                   <  1:5309714/93‑1 (MQ=255)

                                        tCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAttgt                                                 <  2:1038765/93‑1 (MQ=255)

                                        tCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAttgt                                                 <  2:3488091/93‑1 (MQ=255)

                                           caTCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGa                                              <  1:4281095/93‑1 (MQ=255)

                                            aTCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGAt                                             >  1:2716574/1‑93 (MQ=255)

                                             tCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATg                                            <  1:4456280/93‑1 (MQ=255)

                                             tCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATg                                            <  1:3235833/93‑1 (MQ=255)

                                               aCGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGc                                          >  1:5962635/1‑93 (MQ=255)

                                                cGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCa                                         >  1:904672/1‑93 (MQ=255)

                                                    cGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCt                                     >  2:1947920/1‑93 (MQ=255)

                                                     gCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTg                                    >  2:3839174/1‑93 (MQ=255)

                                                     gCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTg                                    <  1:2786362/93‑1 (MQ=255)

                                                       ttttCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCa                                  <  1:5270802/93‑1 (MQ=255)

                                                        tttCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCAt                                 <  2:5248680/93‑1 (MQ=255)

                                                        tttCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCAt                                 <  2:828793/93‑1 (MQ=255)

                                                          tCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTc                               <  1:3748605/93‑1 (MQ=255)

                                                          tCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTc                               <  2:4475153/93‑1 (MQ=255)

                                                              cTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTc                           >  1:6237993/1‑93 (MQ=255)

                                                               tGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCg                          >  2:2764048/1‑93 (MQ=255)

                                                               tGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCg                          >  2:1696107/1‑93 (MQ=255)

                                                                gTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCgg                         >  2:5515453/1‑93 (MQ=255)

                                                                  aGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCTCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGtt                       >  2:4702538/1‑93 (MQ=255)

                                                                   gCCAGTCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGttt                      <  2:637047/93‑1 (MQ=255)

                                                                   gCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGttt                      >  2:2072543/1‑93 (MQ=255)

                                                                   gCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGttt                      >  1:2077674/1‑93 (MQ=255)

                                                                    ccAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTAGGtttt                     >  1:452617/1‑93 (MQ=255)

                                                                     cAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGttttt                    <  1:2249446/93‑1 (MQ=255)

                                                                     cAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGttttt                    <  2:1632013/93‑1 (MQ=255)

                                                                       ggCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTc                  >  2:6514236/1‑93 (MQ=255)

                                                                       ggCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTc                  >  2:2851207/1‑93 (MQ=255)

                                                                         ctctGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCa                <  1:5431669/93‑1 (MQ=255)

                                                                          tctGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCAt               <  1:1952128/93‑1 (MQ=255)

                                                                          tctGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCAt               <  2:5810358/93‑1 (MQ=255)

                                                                           ctGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATg              >  1:177638/1‑93 (MQ=255)

                                                                             gAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGcc            <  1:6174155/93‑1 (MQ=255)

                                                                             gAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGcc            >  2:2230877/1‑93 (MQ=255)

                                                                             gAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGcc            >  2:5569030/1‑93 (MQ=255)

                                                                                aTTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAt         >  1:1627159/1‑93 (MQ=255)

                                                                                aTTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAt         <  2:4592588/93‑1 (MQ=255)

                                                                                aTTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAt         >  2:5034031/1‑93 (MQ=255)

                                                                                 ttGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAtt        >  2:4168577/1‑93 (MQ=255)

                                                                                 ttGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAtt        <  2:257303/93‑1 (MQ=255)

                                                                                 ttGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAtt        >  2:1273079/1‑93 (MQ=255)

                                                                                    atatCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTccc     >  2:6076571/1‑93 (MQ=255)

                                                                                    atatCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTccc     >  2:6606203/1‑93 (MQ=255)

                                                                                    atatCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTccc     >  1:5930034/1‑93 (MQ=255)

                                                                                     tatCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCa    >  1:4826540/1‑93 (MQ=255)

                                                                                     tatCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCa    <  2:3567875/93‑1 (MQ=255)

                                                                                     tatCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCa    <  1:3591023/93‑1 (MQ=255)

                                                                                      atCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCAc   >  1:6662594/1‑93 (MQ=255)

TCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACT  >  NC_000913/2448081‑2448260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACTCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCT  >  NC_000913/2448078‑2448264

TACTCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGC                                                                                               <  SRR4302536.1777083/94‑1 (MQ=60)

TACTCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGC                                                                                               >  SRR4302536.1325660/1‑94 (MQ=60)

 ACTCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCT                                                                                              >  SRR4302536.981565/1‑94 (MQ=60)

  CTCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTA                                                                                             >  SRR4302536.2458532/1‑94 (MQ=60)

  CTCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTA                                                                                             >  SRR4302536.3801162/1‑94 (MQ=60)

    CAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGCAATTGATATCGCTATT                                                                                           >  SRR4302536.2156434/1‑94 (MQ=60)

    CAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATT                                                                                           <  SRR4302536.5802653/94‑1 (MQ=60)

     AAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTG                                                                                          <  SRR4302536.5888611/94‑1 (MQ=60)

      AATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGC                                                                                         >  SRR4302536.2867179/1‑94 (MQ=60)

      AATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAACCAGGCTCTGAAATTGATATCGCTATTGC                                                                                         >  SRR4302536.6793528/1‑94 (MQ=60)

       ATGTCGCAGTTAATGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCC                                                                                        >  SRR4302536.5504059/1‑94 (MQ=60)

        TGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCA                                                                                       <  SRR4302536.6799866/94‑1 (MQ=60)

         GTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCAT                                                                                      <  SRR4302536.4709101/94‑1 (MQ=60)

          TCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATT                                                                                     <  SRR4302536.5777593/94‑1 (MQ=60)

           CGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCCTTTCGCCTGTAGCCACGCTCTGAAATTGATATCTCTATTGCCATTG                                                                                    >  SRR4302536.5535632/1‑94 (MQ=60)

             CAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGT                                                                                  <  SRR4302536.2636633/94‑1 (MQ=60)

              AGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTT                                                                                 >  SRR4302536.2323350/1‑94 (MQ=60)

              AGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTT                                                                                 >  SRR4302536.6377220/1‑94 (MQ=60)

                 TAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGC                                                                              >  SRR4302536.2675841/1‑94 (MQ=60)

                 TAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTACC                                                                              <  SRR4302536.6628745/94‑1 (MQ=60)

                  AAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCG                                                                             >  SRR4302536.1380448/1‑94 (MQ=60)

                   AAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGC                                                                            >  SRR4302536.869267/1‑94 (MQ=60)

                   AAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGC                                                                            >  SRR4302536.1679517/1‑94 (MQ=60)

                    AGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCA                                                                           >  SRR4302536.1500916/1‑94 (MQ=60)

                     GAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAA                                                                          >  SRR4302536.6744726/1‑94 (MQ=60)

                     GAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAA                                                                          >  SRR4302536.4857221/1‑94 (MQ=60)

                     GAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAA                                                                          <  SRR4302536.1297573/94‑1 (MQ=60)

                      AGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAA                                                                         >  SRR4302536.5548163/1‑94 (MQ=60)

                        GCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGG                                                                       >  SRR4302536.856416/1‑94 (MQ=60)

                         CGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGT                                                                      <  SRR4302536.4618655/94‑1 (MQ=60)

                         CGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGT                                                                      <  SRR4302536.470477/94‑1 (MQ=60)

                          GGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTC                                                                     <  SRR4302536.2772346/94‑1 (MQ=60)

                          GGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTC                                                                     <  SRR4302536.73380/94‑1 (MQ=60)

                          GGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTC                                                                     <  SRR4302536.506400/94‑1 (MQ=60)

                          GGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTC                                                                     >  SRR4302536.5034934/1‑94 (MQ=60)

                          GGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTC                                                                     <  SRR4302536.4962262/94‑1 (MQ=60)

                           GTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCG                                                                    <  SRR4302536.2392891/94‑1 (MQ=60)

                           GTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCG                                                                    <  SRR4302536.5851905/94‑1 (MQ=60)

                            TAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGC                                                                   >  SRR4302536.371640/1‑94 (MQ=60)

                             AAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCC                                                                  >  SRR4302536.800153/1‑94 (MQ=60)

                             AAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCC                                                                  <  SRR4302536.4937553/94‑1 (MQ=60)

                              AATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCC                                                                 <  SRR4302536.2167785/94‑1 (MQ=60)

                                 TCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCAT                                                              >  SRR4302536.3543691/1‑94 (MQ=60)

                                  CACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATT                                                             <  SRR4302536.6633894/94‑1 (MQ=60)

                                  CACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATT                                                             <  SRR4302536.6822220/94‑1 (MQ=60)

                                   CCCGATCGTCACATCACGGCGGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            <  SRR4302536.5478561/94‑1 (MQ=60)

                                   AGCGATCGTCACATCACTGCCGCTTTTCGGCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            <  SRR4302536.3457210/94‑1 (MQ=60)

                                   ACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            <  SRR4302536.4063991/94‑1 (MQ=60)

                                   ACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            >  SRR4302536.4176308/1‑94 (MQ=60)

                                   ACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            <  SRR4302536.359218/94‑1 (MQ=60)

                                   ACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            <  SRR4302536.3072299/94‑1 (MQ=60)

                                   ACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            >  SRR4302536.770173/1‑94 (MQ=60)

                                   ACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTT                                                            <  SRR4302536.1480272/94‑1 (MQ=60)

                                    CCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTG                                                           <  SRR4302536.5504085/94‑1 (MQ=60)

                                     CGATCTTCACATCACGGCAGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATAGCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGT                                                          <  SRR4302536.2019653/94‑1 (MQ=60)

                                     CGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGT                                                          <  SRR4302536.1129151/94‑1 (MQ=60)

                                     CGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGT                                                          <  SRR4302536.81030/94‑1 (MQ=60)

                                     CGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGT                                                          <  SRR4302536.1631712/94‑1 (MQ=60)

                                     CGATCGTCACATCACGGCCGATTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGT                                                          >  SRR4302536.6704212/1‑94 (MQ=60)

                                      GATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTA                                                         >  SRR4302536.2122238/1‑94 (MQ=60)

                                       ATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAT                                                        >  SRR4302536.197454/1‑94 (MQ=60)

                                       ATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTAT                                                        <  SRR4302536.5825097/94‑1 (MQ=60)

                                        TCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATT                                                       <  SRR4302536.6166318/94‑1 (MQ=60)

                                        TCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATT                                                       <  SRR4302536.5841891/94‑1 (MQ=60)

                                         CGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTG                                                      <  SRR4302536.6546429/94‑1 (MQ=60)

                                         CGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTG                                                      <  SRR4302536.5468186/94‑1 (MQ=60)

                                         CGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTG                                                      >  SRR4302536.2137955/1‑94 (MQ=60)

                                           TCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTT                                                    <  SRR4302536.2864263/94‑1 (MQ=60)

                                              CATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGAT                                                 >  SRR4302536.2797596/1‑94 (MQ=60)

                                              CATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGAT                                                 <  SRR4302536.4408837/94‑1 (MQ=60)

                                               ATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATG                                                <  SRR4302536.4736317/94‑1 (MQ=60)

                                                TCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGG                                               <  SRR4302536.3330686/94‑1 (MQ=60)

                                                TCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGG                                               <  SRR4302536.4590167/94‑1 (MQ=60)

                                                 CACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGC                                              >  SRR4302536.6140344/1‑94 (MQ=60)

                                                  ACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCA                                             >  SRR4302536.931338/1‑94 (MQ=60)

                                                  ACGGCCGCTTTTAGGCTGTAGCCAGGCTCTGAAAATGATATCGCTATTGTCATTGGTTAGCGCAGAAGTCGCCACATCTGTATTGTTGATGGCA                                             >  SRR4302536.6846617/1‑94 (MQ=53)

                                                      CCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCT                                         >  SRR4302536.3648451/1‑94 (MQ=60)

                                                       CGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTG                                        >  SRR4302536.5711439/1‑94 (MQ=60)

                                                       CGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTG                                        >  SRR4302536.5532974/1‑94 (MQ=60)

                                                       CGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTG                                        >  SRR4302536.4130475/1‑94 (MQ=60)

                                                        GCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGC                                       <  SRR4302536.2869106/94‑1 (MQ=60)

                                                         CTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCA                                      <  SRR4302536.368985/94‑1 (MQ=60)

                                                         CTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCA                                      <  SRR4302536.4208246/94‑1 (MQ=60)

                                                          TTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCAT                                     <  SRR4302536.5427894/94‑1 (MQ=60)

                                                          TTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCAT                                     <  SRR4302536.6216640/94‑1 (MQ=60)

                                                           TTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGGTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATT                                    <  SRR4302536.5987298/94‑1 (MQ=60)

                                                            TTCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTC                                   >  SRR4302536.6848950/1‑94 (MQ=60)

                                                             TCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCC                                  >  SRR4302536.1531587/1‑94 (MQ=60)

                                                             TCGCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCC                                  <  SRR4302536.3860657/94‑1 (MQ=60)

                                                               GCCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGT                                <  SRR4302536.3326289/94‑1 (MQ=60)

                                                                CCTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTC                               >  SRR4302536.6423826/1‑94 (MQ=60)

                                                                 CTGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCG                              >  SRR4302536.2507586/1‑94 (MQ=60)

                                                                  TGTAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAACGCAAAGGTCGCCCCATTTGCATTGTTGATGCCAAACTGCATTCCGTGGG                             >  SRR4302536.4055025/1‑94 (MQ=60)

                                                                    TAGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTT                           <  SRR4302536.5019124/94‑1 (MQ=60)

                                                                     AGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTT                          >  SRR4302536.2140245/1‑94 (MQ=60)

                                                                     AGCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTT                          <  SRR4302536.3668700/94‑1 (MQ=60)

                                                                      GCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTT                         <  SRR4302536.5283572/94‑1 (MQ=60)

                                                                      GCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTT                         <  SRR4302536.4968028/94‑1 (MQ=60)

                                                                      GCCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTAGGTTTT                         >  SRR4302536.464500/1‑94 (MQ=60)

                                                                       CCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTT                        <  SRR4302536.5433699/94‑1 (MQ=60)

                                                                       CCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTT                        >  SRR4302536.4566908/1‑94 (MQ=60)

                                                                       CCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTT                        >  SRR4302536.6159101/1‑94 (MQ=60)

                                                                       CCAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTT                        >  SRR4302536.4700898/1‑94 (MQ=60)

                                                                        CAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTT                       <  SRR4302536.2317543/94‑1 (MQ=60)

                                                                        CAGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTT                       <  SRR4302536.2492122/94‑1 (MQ=60)

                                                                         AGGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTC                      >  SRR4302536.4915667/1‑94 (MQ=60)

                                                                          GGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCC                     <  SRR4302536.2113503/94‑1 (MQ=60)

                                                                          GGCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCC                     <  SRR4302536.4359898/94‑1 (MQ=60)

                                                                           GCTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCA                    <  SRR4302536.2939686/94‑1 (MQ=60)

                                                                            CTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCAT                   >  SRR4302536.2442220/1‑94 (MQ=60)

                                                                            CTCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCAT                   <  SRR4302536.5594077/94‑1 (MQ=60)

                                                                            CTCTGAAATTGATATCGCTATTGCCATTAGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCAT                   >  SRR4302536.3883364/1‑94 (MQ=60)

                                                                             TCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATG                  <  SRR4302536.2010839/94‑1 (MQ=60)

                                                                             TCTGAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATG                  >  SRR4302536.182311/1‑94 (MQ=60)

                                                                                GAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCA               <  SRR4302536.6358052/94‑1 (MQ=60)

                                                                                GAAATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCA               >  SRR4302536.6203652/1‑94 (MQ=60)

                                                                                  AATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAT             >  SRR4302536.6037118/1‑94 (MQ=60)

                                                                                  AATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAT             <  SRR4302536.3364825/94‑1 (MQ=60)

                                                                                  AATTGATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAAT             >  SRR4302536.1674525/1‑94 (MQ=60)

                                                                                      GATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCC         >  SRR4302536.6106681/1‑94 (MQ=60)

                                                                                       ATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCA        >  SRR4302536.278036/1‑94 (MQ=60)

                                                                                       ATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCA        <  SRR4302536.3174000/94‑1 (MQ=60)

                                                                                       ATATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCA        >  SRR4302536.4971796/1‑94 (MQ=60)

                                                                                        TATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCAC       <  SRR4302536.3698443/94‑1 (MQ=60)

                                                                                        TATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCAC       >  SRR4302536.6860611/1‑94 (MQ=60)

                                                                                         ATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACT      <  SRR4302536.1024991/94‑1 (MQ=60)

                                                                                         ATCGCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACT      >  SRR4302536.6125677/1‑94 (MQ=60)

                                                                                            GCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCC   <  SRR4302536.1141891/94‑1 (MQ=60)

                                                                                            GCTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCC   >  SRR4302536.632666/1‑94 (MQ=60)

                                                                                            GCTATTGCCATTGGTTAGAGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCC   >  SRR4302536.1648498/1‑94 (MQ=60)

                                                                                             CTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCT  >  SRR4302536.278509/1‑94 (MQ=60)

TACTCAAATGTCGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCT  >  NC_000913/2448078‑2448264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`