Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,052,844	T→C	I5M (ATA→ATG)	glnG ←	GlnG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,052,844	0	T	C	99.6%	778.9 / NA	239	I5M (ATA→ATG)	glnG	GlnG
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (108/130); minor base A (1/0); total (109/130)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT  >  NC_000913/4052754‑4052933

gTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAt                                                                                         >  2:2989758/1‑93 (MQ=255)

gTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAt                                                                                         >  1:165437/1‑93 (MQ=255)

cttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAt                                                                                         <  1:1703905/92‑1 (MQ=255)

 ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTAGCCAGACCATc                                                                                        >  1:4048255/1‑93 (MQ=255)

   ctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATccc                                                                                      <  2:77047/93‑1 (MQ=255)

     cAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTc                                                                                    >  1:2672350/1‑93 (MQ=255)

      aaaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCg                                                                                   >  1:3938159/1‑93 (MQ=255)

       aaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGt                                                                                  >  1:2564384/1‑93 (MQ=255)

        aCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGtt                                                                                 >  2:5556783/1‑93 (MQ=255)

           tcgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCa                                                                              <  1:855689/93‑1 (MQ=255)

             gtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATa                                                                            >  1:4499759/1‑93 (MQ=255)

               aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATaaa                                                                          <  2:518135/93‑1 (MQ=255)

               aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATaaa                                                                          <  2:6113615/93‑1 (MQ=255)

                    ttAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCa                                                                     <  1:4341523/93‑1 (MQ=255)

                      aaaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCAcc                                                                   >  2:115130/1‑93 (MQ=255)

                      aaaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCAcc                                                                   >  1:2796635/1‑93 (MQ=255)

                       acccTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTCCCCAGACCATCCCTCGTTGCATAAACGTCACCt                                                                  <  1:1930611/91‑1 (MQ=255)

                       aaCCTGCCCCGGAGAGCCCACGTTCAAGCCCCCAACGGATGGAAGTATCGTCATCGACTCCCCAGACCATCCCTCGTTGCATAAACGTCACCt                                                                  <  1:6155870/93‑1 (MQ=255)

                       aaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCt                                                                  >  1:6380732/1‑93 (MQ=255)

                       aaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGACACCt                                                                  >  2:2477119/1‑93 (MQ=255)

                        tccTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCtt                                                                 >  1:6436210/2‑93 (MQ=255)

                        aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCtt                                                                 <  1:112243/93‑1 (MQ=255)

                        aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCtt                                                                 >  1:6716401/1‑93 (MQ=255)

                        aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCtt                                                                 <  2:1829265/93‑1 (MQ=255)

                         ccTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCttt                                                                >  1:528754/1‑93 (MQ=255)

                         ccTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCttt                                                                <  1:1002157/93‑1 (MQ=255)

                          cTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTa                                                               >  1:352707/1‑93 (MQ=255)

                          cTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTa                                                               >  2:4983098/1‑93 (MQ=255)

                          cTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTa                                                               <  1:6498457/93‑1 (MQ=255)

                           tGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAt                                                              <  1:1167978/93‑1 (MQ=255)

                           cgCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAt                                                              <  1:4995313/92‑1 (MQ=255)

                             ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAttt                                                            >  1:4170268/1‑93 (MQ=255)

                              cccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTc                                                           >  1:2944103/1‑93 (MQ=255)

                              cccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTc                                                           <  1:1873029/93‑1 (MQ=255)

                              cccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTc                                                           <  1:1584463/93‑1 (MQ=255)

                               ccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTcc                                                          <  2:1675893/93‑1 (MQ=255)

                                cAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                         <  1:4746675/93‑1 (MQ=255)

                                cAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                         <  2:4186603/93‑1 (MQ=255)

                                cAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                         >  1:5781125/1‑93 (MQ=255)

                                 agcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTg                                                        <  1:3667213/93‑1 (MQ=255)

                                  gcgagcgCACGTTCAAGCACCCATCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGa                                                       <  1:3140059/93‑1 (MQ=255)

                                   cgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGAt                                                      <  1:3344571/93‑1 (MQ=255)

                                    gagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATa                                                     >  1:327899/1‑93 (MQ=255)

                                    gagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATa                                                     >  1:4680773/1‑93 (MQ=255)

                                     ggcgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAg                                                    <  1:4005439/92‑1 (MQ=255)

                                     agcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAg                                                    >  1:443473/1‑93 (MQ=255)

                                      gcgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAgg                                                   >  1:6630301/1‑93 (MQ=255)

                                       cgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGc                                                  <  1:5681016/93‑1 (MQ=255)

                                        gcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCa                                                 <  1:876947/93‑1 (MQ=255)

                                         cACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAg                                                >  1:3874320/1‑93 (MQ=255)

                                          ccGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGCCCATCCCTCGTTGCATAAACGTAACCTTTATTTCCTGATAGGCAgg                                               <  1:5370143/92‑1 (MQ=255)

                                          ccGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAgg                                               <  1:1557246/92‑1 (MQ=255)

                                          aCGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAgg                                               >  2:6385826/1‑93 (MQ=255)

                                           cGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGt                                              <  2:4368869/93‑1 (MQ=255)

                                           cGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGt                                              <  1:2908495/93‑1 (MQ=255)

                                            gTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTa                                             <  1:647495/93‑1 (MQ=255)

                                            gTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTa                                             <  2:1418111/93‑1 (MQ=255)

                                               cAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                          <  2:3708602/93‑1 (MQ=255)

                                               cAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                          >  2:4794607/1‑93 (MQ=255)

                                                aaGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAcc                                         >  1:4911832/1‑93 (MQ=255)

                                                 aGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCg                                        <  1:552650/93‑1 (MQ=255)

                                                 aGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCg                                        >  1:6188083/1‑93 (MQ=255)

                                                  gCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCga                                       >  2:6288261/1‑93 (MQ=255)

                                                    aCCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCgaga                                     >  2:5087963/1‑93 (MQ=255)

                                                      ccAACGGATGGAACTATCGTCATCGACTACCCAGACCAACCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAc                                   <  1:3605252/93‑1 (MQ=255)

                                                        aaCGGATGTAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTc                                 <  2:4015072/93‑1 (MQ=255)

                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                >  2:727099/1‑93 (MQ=255)

                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                <  1:3506822/93‑1 (MQ=255)

                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                >  2:5663608/1‑93 (MQ=255)

                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                <  2:3059176/93‑1 (MQ=255)

                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                >  2:4340890/1‑93 (MQ=255)

                                                          cGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCgg                               >  1:2204459/1‑93 (MQ=255)

                                                          cGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCgg                               <  1:799445/93‑1 (MQ=255)

                                                           ggATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGt                              >  1:4800686/1‑93 (MQ=255)

                                                           ggATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGt                              >  2:5926859/1‑93 (MQ=255)

                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             <  2:3618506/93‑1 (MQ=255)

                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             >  2:289330/1‑93 (MQ=255)

                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             <  2:4707111/93‑1 (MQ=255)

                                                               ggAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGc                          <  2:2205003/93‑1 (MQ=255)

                                                               ggAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGc                          >  2:5643662/1‑93 (MQ=255)

                                                                 aaCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGccc                        <  1:5350863/93‑1 (MQ=255)

                                                                  aCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCt                       <  2:3424603/93‑1 (MQ=255)

                                                                  aCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCt                       <  2:5852748/93‑1 (MQ=255)

                                                                   cTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTg                      <  1:5933930/93‑1 (MQ=255)

                                                                   cTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTg                      <  1:4648429/93‑1 (MQ=255)

                                                                     aTCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGc                    <  2:190550/93‑1 (MQ=255)

                                                                       cGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCa                  <  2:4809905/93‑1 (MQ=255)

                                                                        gTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCaa                 >  2:2389864/1‑93 (MQ=255)

                                                                         tCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc                <  2:3120899/93‑1 (MQ=255)

                                                                         tCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc                >  2:3530652/1‑93 (MQ=255)

                                                                             cGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGt            >  2:2181466/1‑93 (MQ=255)

                                                                               aCTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaa          >  1:6437265/1‑93 (MQ=255)

                                                                                  aCCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAtt       >  1:3809407/1‑93 (MQ=255)

                                                                                  aCCCAGACCATCCATCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAtt       <  2:3392005/93‑1 (MQ=255)

                                                                                   cccAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTc      <  1:3109091/93‑1 (MQ=255)

                                                                                    ccAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCa     >  1:2160772/1‑93 (MQ=255)

                                                                                      aGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAt   <  2:6422912/93‑1 (MQ=255)

                                                                                       gACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt  >  1:6014689/1‑93 (MQ=255)

                                                                                       gACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt  >  1:251609/1‑93 (MQ=255)

                                                                                       gACAATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGGATGCCCTGGCCAACTGGTAAATTCAAtt  >  2:3767210/1‑93 (MQ=255)

GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT  >  NC_000913/4052754‑4052933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTT  >  NC_000913/4052751‑4052934

GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACC                                                                                            >  SRR4302536.2270022/1‑94 (MQ=60)

 CCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCA                                                                                           <  SRR4302536.715732/94‑1 (MQ=60)

 CCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCA                                                                                           >  SRR4302536.2813320/1‑94 (MQ=60)

  CGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAT                                                                                          >  SRR4302536.169752/1‑94 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTAGCCAGACCATC                                                                                         >  SRR4302536.4168249/1‑94 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                         >  SRR4302536.6685352/1‑94 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                         >  SRR4302536.4113619/1‑94 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                         >  SRR4302536.784158/1‑94 (MQ=60)

   GTCCTAAAACGTCGTACAGGTTAAACCTCCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                         <  SRR4302536.4906845/94‑1 (MQ=60)

   CTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                         <  SRR4302536.1753201/94‑1 (MQ=60)

    TTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCC                                                                                        <  SRR4302536.6667056/94‑1 (MQ=60)

     TCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCC                                                                                       >  SRR4302536.3949899/1‑94 (MQ=60)

      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCT                                                                                      >  SRR4302536.3645901/1‑94 (MQ=60)

      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCT                                                                                      <  SRR4302536.2742205/94‑1 (MQ=60)

      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCT                                                                                      <  SRR4302536.3880425/94‑1 (MQ=60)

       TCAAACGTCGTACAGGTTAACCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTC                                                                                     <  SRR4302536.1263956/94‑1 (MQ=60)

       TCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTC                                                                                     >  SRR4302536.2752248/1‑94 (MQ=60)

       TCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTC                                                                                     >  SRR4302536.3212070/1‑94 (MQ=60)

       TCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTC                                                                                     <  SRR4302536.6066744/94‑1 (MQ=60)

       TCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTC                                                                                     <  SRR4302536.1960820/94‑1 (MQ=60)

       ACAAACGTCGTACAGTATAAACCTGCCCCAGCGAGCGCACGTTCAAGCCCCCAACGGATGGAACTATCGTCATCGATTACCCAGACCATCCCTC                                                                                     <  SRR4302536.4937647/94‑1 (MQ=60)

        CAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCG                                                                                    >  SRR4302536.4055173/1‑94 (MQ=60)

         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGT                                                                                   >  SRR4302536.2641540/1‑94 (MQ=60)

         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGT                                                                                   <  SRR4302536.6324403/94‑1 (MQ=60)

          AACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTT                                                                                  <  SRR4302536.887991/94‑1 (MQ=60)

          AACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTT                                                                                  <  SRR4302536.4480324/94‑1 (MQ=60)

          AACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTAACCAGACCATCCCTCGTT                                                                                  >  SRR4302536.671863/1‑94 (MQ=60)

           ACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTG                                                                                 <  SRR4302536.4803278/94‑1 (MQ=60)

             GTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCA                                                                               >  SRR4302536.3581911/1‑94 (MQ=60)

             GTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCA                                                                               <  SRR4302536.3287165/94‑1 (MQ=60)

              TCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCAT                                                                              <  SRR4302536.880848/94‑1 (MQ=60)

               CGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATA                                                                             >  SRR4302536.4634802/1‑94 (MQ=60)

               CGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATA                                                                             >  SRR4302536.3047395/1‑94 (MQ=60)

                  ACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAC                                                                          >  SRR4302536.5520746/1‑94 (MQ=60)

                   CAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATTGAACTATCGTCATCTACTACCCAGACCATCCCTCGTTGCATAAACG                                                                         >  SRR4302536.6929171/1‑94 (MQ=60)

                   CAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACG                                                                         >  SRR4302536.4145399/1‑94 (MQ=60)

                    AGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGT                                                                        <  SRR4302536.834341/94‑1 (MQ=60)

                       TTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCAC                                                                     <  SRR4302536.4471523/94‑1 (MQ=60)

                        TAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACC                                                                    >  SRR4302536.2879596/1‑94 (MQ=60)

                        TAAACCTCCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACC                                                                    <  SRR4302536.5483652/94‑1 (MQ=60)

                         AAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCT                                                                   >  SRR4302536.6570946/1‑94 (MQ=60)

                          ATCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  >  SRR4302536.6628565/1‑94 (MQ=60)

                          AGCCTCCCCCACCGAGCACAGGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  <  SRR4302536.4444923/94‑1 (MQ=60)

                          ACCCTGCCCCCGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCCGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  <  SRR4302536.3858741/94‑1 (MQ=60)

                          ACCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTCCCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  <  SRR4302536.1988678/94‑1 (MQ=60)

                          AACCTGCCCCGGAGAGCCCACGTTCAAGCCCCCAACGGATGGAAGTATCGTCATCGACTCCCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  <  SRR4302536.6339265/94‑1 (MQ=60)

                          AACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  >  SRR4302536.6915773/1‑94 (MQ=60)

                          AACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  <  SRR4302536.4824402/94‑1 (MQ=60)

                          AACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                  <  SRR4302536.4540359/94‑1 (MQ=60)

                           ACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGACCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTT                                                                 <  SRR4302536.242983/94‑1 (MQ=60)

                           ACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTT                                                                 <  SRR4302536.115217/94‑1 (MQ=60)

                           ACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTT                                                                 >  SRR4302536.542278/1‑94 (MQ=60)

                            CCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTA                                                                <  SRR4302536.1031731/94‑1 (MQ=60)

                            CCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTA                                                                >  SRR4302536.1689679/1‑94 (MQ=60)

                            CCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTA                                                                >  SRR4302536.362048/1‑94 (MQ=60)

                             CTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAT                                                               <  SRR4302536.6692173/94‑1 (MQ=60)

                              TGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATT                                                              <  SRR4302536.1203132/94‑1 (MQ=60)

                              CGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATT                                                              <  SRR4302536.5145246/94‑1 (MQ=60)

                               GCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTT                                                             >  SRR4302536.4294156/1‑94 (MQ=60)

                               GCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTT                                                             >  SRR4302536.2910072/1‑94 (MQ=60)

                                CCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTC                                                            >  SRR4302536.5161149/1‑94 (MQ=60)

                                CCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTC                                                            >  SRR4302536.3030897/1‑94 (MQ=60)

                                CCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTC                                                            <  SRR4302536.4006473/94‑1 (MQ=60)

                                 CCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCC                                                           <  SRR4302536.1630844/94‑1 (MQ=60)

                                 CCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCC                                                           >  SRR4302536.1614023/1‑94 (MQ=60)

                                 CCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCC                                                           <  SRR4302536.1929410/94‑1 (MQ=60)

                                 CCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCC                                                           >  SRR4302536.6616309/1‑94 (MQ=60)

                                  CCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCT                                                          >  SRR4302536.5953184/1‑94 (MQ=60)

                                   CAGCGAGCGCACGTTCAAGCACGCAACGGATGGAACTATCGTCATCGACTACCCAGCCCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTG                                                         <  SRR4302536.3775821/94‑1 (MQ=60)

                                   CAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTG                                                         <  SRR4302536.4889527/94‑1 (MQ=60)

                                    AGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGA                                                        <  SRR4302536.3776851/94‑1 (MQ=60)

                                    AGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGA                                                        <  SRR4302536.1286428/94‑1 (MQ=60)

                                     GCGAGCGCACGTTCAAGCACCCATCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGAT                                                       <  SRR4302536.3231969/94‑1 (MQ=60)

                                     GCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGAT                                                       >  SRR4302536.4188396/1‑94 (MQ=60)

                                      CGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATA                                                      <  SRR4302536.471686/94‑1 (MQ=60)

                                      CGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATA                                                      <  SRR4302536.3443675/94‑1 (MQ=60)

                                      CGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATA                                                      >  SRR4302536.4821542/1‑94 (MQ=60)

                                      CGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATA                                                      >  SRR4302536.336606/1‑94 (MQ=60)

                                       GAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAG                                                     <  SRR4302536.3302973/94‑1 (MQ=60)

                                       GAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAG                                                     >  SRR4302536.455150/1‑94 (MQ=60)

                                        GGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGG                                                    <  SRR4302536.4124297/94‑1 (MQ=60)

                                        AGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGG                                                    <  SRR4302536.1755686/94‑1 (MQ=60)

                                        AGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGG                                                    >  SRR4302536.6827516/1‑94 (MQ=60)

                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                  <  SRR4302536.4747445/94‑1 (MQ=60)

                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                  <  SRR4302536.5942898/94‑1 (MQ=60)

                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                  <  SRR4302536.5849923/94‑1 (MQ=60)

                                           GCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAG                                                 <  SRR4302536.902782/94‑1 (MQ=60)

                                           GCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAG                                                 >  SRR4302536.3989548/1‑94 (MQ=60)

                                             CCGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGCCCATCCCTCGTTGCATAAACGTAACCTTTATTTCCTGATAGGCAGGT                                               <  SRR4302536.5530329/94‑1 (MQ=60)

                                             CCGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGT                                               <  SRR4302536.1602982/94‑1 (MQ=60)

                                              CGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTA                                              <  SRR4302536.2994340/94‑1 (MQ=60)

                                              CGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTA                                              >  SRR4302536.1749823/1‑94 (MQ=60)

                                              CGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTA                                              <  SRR4302536.6432068/94‑1 (MQ=60)

                                               GTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAA                                             <  SRR4302536.666541/94‑1 (MQ=60)

                                                  CAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACC                                          >  SRR4302536.5059478/1‑94 (MQ=60)

                                                   AAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCG                                         >  SRR4302536.6372386/1‑94 (MQ=60)

                                                   AAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGGAAACCG                                         >  SRR4302536.3650721/1‑94 (MQ=60)

                                                    AGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAGCGA                                        >  SRR4302536.3161141/1‑94 (MQ=60)

                                                    AGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGA                                        <  SRR4302536.566733/94‑1 (MQ=60)

                                                    AGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGA                                        >  SRR4302536.2689381/1‑94 (MQ=60)

                                                      CACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTCTATTTCCTGCTACGCAGGTAAACCGAGA                                      >  SRR4302536.483954/1‑94 (MQ=60)

                                                        CCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAC                                    <  SRR4302536.171107/94‑1 (MQ=60)

                                                         CCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACT                                   <  SRR4302536.4123215/94‑1 (MQ=60)

                                                         CCAACGGATGGAACTATCGTCATCGACTACCCAGACCAACCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACT                                   <  SRR4302536.3713062/94‑1 (MQ=60)

                                                           AACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCG                                 <  SRR4302536.2837421/94‑1 (MQ=60)

                                                            ACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGG                                >  SRR4302536.2271020/1‑94 (MQ=60)

                                                            ACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGG                                <  SRR4302536.3611486/94‑1 (MQ=60)

                                                            ACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGG                                <  SRR4302536.6103012/94‑1 (MQ=60)

                                                             CGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGT                               >  SRR4302536.4945175/1‑94 (MQ=60)

                                                             CGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGT                               <  SRR4302536.822888/94‑1 (MQ=60)

                                                              GGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTA                              <  SRR4302536.51716/94‑1 (MQ=60)

                                                                ATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATG                            <  SRR4302536.5678037/94‑1 (MQ=60)

                                                                    AACTATCGTCATCGACTACCCCGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                        <  SRR4302536.5817786/94‑1 (MQ=60)

                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                        >  SRR4302536.1152662/1‑94 (MQ=60)

                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                        <  SRR4302536.5510596/94‑1 (MQ=60)

                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                        <  SRR4302536.5140344/94‑1 (MQ=60)

                                                                     ACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTG                       >  SRR4302536.6333695/1‑94 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                      <  SRR4302536.4788184/94‑1 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                      <  SRR4302536.6110684/94‑1 (MQ=60)

                                                                       TATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGC                     >  SRR4302536.6419753/1‑94 (MQ=60)

                                                                        ATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCC                    >  SRR4302536.2901740/1‑94 (MQ=60)

                                                                           GTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAC                 <  SRR4302536.6650971/94‑1 (MQ=60)

                                                                            TCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACT                <  SRR4302536.4588487/94‑1 (MQ=60)

                                                                            TCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACT                <  SRR4302536.4292141/94‑1 (MQ=60)

                                                                             CATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTG               >  SRR4302536.1195165/1‑94 (MQ=60)

                                                                              ATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGG              <  SRR4302536.2426413/94‑1 (MQ=60)

                                                                               TCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGT             <  SRR4302536.5018316/94‑1 (MQ=60)

                                                                               TCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGT             <  SRR4302536.2047744/94‑1 (MQ=60)

                                                                               TCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGT             >  SRR4302536.2604899/1‑94 (MQ=60)

                                                                                CGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTA            >  SRR4302536.549495/1‑94 (MQ=60)

                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA           >  SRR4302536.1999927/1‑94 (MQ=60)

                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA           >  SRR4302536.6629647/1‑94 (MQ=60)

                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA           >  SRR4302536.6629648/1‑94 (MQ=60)

                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA           <  SRR4302536.3378963/94‑1 (MQ=60)

                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT         >  SRR4302536.902090/1‑94 (MQ=60)

                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT         <  SRR4302536.1790495/94‑1 (MQ=60)

                                                                                    TACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATT        >  SRR4302536.4998751/1‑94 (MQ=60)

                                                                                    TACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATT        >  SRR4302536.3922960/1‑94 (MQ=60)

                                                                                     ACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTC       >  SRR4302536.6880270/1‑94 (MQ=60)

                                                                                      CCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCA      <  SRR4302536.3199876/94‑1 (MQ=60)

                                                                                      CCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCA      >  SRR4302536.2225766/1‑94 (MQ=60)

                                                                                      CCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCA      >  SRR4302536.3494642/1‑94 (MQ=60)

                                                                                       CCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTTGCCAACTGtgtaatttaa     >  SRR4302536.4214859/1‑84 (MQ=60)

                                                                                       CCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAA     >  SRR4302536.5818261/1‑94 (MQ=60)

                                                                                        CAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAT    <  SRR4302536.3816850/94‑1 (MQ=60)

                                                                                        CAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAT    <  SRR4302536.300006/94‑1 (MQ=60)

                                                                                         AGACCATCCGTCGTTGCATACACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   >  SRR4302536.6761949/1‑94 (MQ=60)

                                                                                         AGACCATCCCTGGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   <  SRR4302536.1698708/94‑1 (MQ=60)

                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   <  SRR4302536.4843089/94‑1 (MQ=60)

                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   <  SRR4302536.224178/94‑1 (MQ=60)

                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   >  SRR4302536.258325/1‑94 (MQ=60)

                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   >  SRR4302536.360992/1‑94 (MQ=60)

                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   >  SRR4302536.6193997/1‑94 (MQ=60)

                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT   <  SRR4302536.6141144/94‑1 (MQ=60)

                                                                                          GACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTT  >  SRR4302536.2899060/1‑94 (MQ=60)

                                                                                          GACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTT  >  SRR4302536.5541105/1‑94 (MQ=60)

                                                                                          GACCATCCCTCGTTGCATAAACGTCACCTTTATTCCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTT  <  SRR4302536.6361025/94‑1 (MQ=60)

GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTT  >  NC_000913/4052751‑4052934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`