Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,050	A→G	E1215G (GAG→GGG)	metH →	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,050	0	A	G	100.0%	848.4 / NA	257	E1215G (GAG→GGG)	metH	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (131/126); total (131/126)

Only 100 of 265 total aligned reads displayed.

CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAG  >  NC_000913/4224960‑4225138

cGGACAGCAAGTACTACGCTGTAGCACAATTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGgc                                                                                        <  2:138198/93‑1 (MQ=255)

cGGACAGAAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGgc                                                                                        <  1:4369684/93‑1 (MQ=255)

  gACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGgcgc                                                                                      >  2:3753823/1‑93 (MQ=255)

   aaaGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCGGTTTAAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgct                                                                                     <  1:1091243/91‑1 (MQ=255)

   aCAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgct                                                                                     >  1:2446911/1‑93 (MQ=255)

    cAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAACGGTATGAGCGTTACCGAAGTTGGGCgctg                                                                                    >  1:4273156/1‑93 (MQ=255)

      ggaaGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGCGCgctggc                                                                                  >  1:6414555/3‑93 (MQ=255)

      gCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctggc                                                                                  >  1:3324802/1‑93 (MQ=255)

      gCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctggc                                                                                  >  2:946032/1‑93 (MQ=255)

        aaGTACGACGCTGTAGCACAAATTGAGCACGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctggctg                                                                                <  2:1773987/93‑1 (MQ=255)

         aGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGctggctgg                                                                               >  1:473521/1‑93 (MQ=255)

          gTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCtggctggc                                                                              >  1:3124513/1‑93 (MQ=255)

            actacGCTGTAGCACAAATTCAGCGCAATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAc                                                                            <  2:694460/93‑1 (MQ=255)

             ctacGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAcc                                                                           <  2:4531290/93‑1 (MQ=255)

              tacGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCg                                                                          >  2:4819315/1‑93 (MQ=255)

                cGCTGTAGCACAAATTAAGCGCAATCAGGTTGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGaa                                                                        <  1:6323445/93‑1 (MQ=255)

                  cgggaGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATc                                                                      <  2:2479146/89‑1 (MQ=255)

                  cTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATc                                                                      >  1:1352495/1‑93 (MQ=255)

                  cTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATc                                                                      >  1:3397863/1‑93 (MQ=255)

                   tGTAGCACAAATTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCt                                                                     <  2:4763072/93‑1 (MQ=255)

                    gTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTg                                                                    >  2:2583692/1‑93 (MQ=255)

                     tAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTgg                                                                   <  2:6394954/93‑1 (MQ=255)

                      aGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTggg                                                                  >  2:1643704/1‑93 (MQ=255)

                      aGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTggg                                                                  <  1:4141714/93‑1 (MQ=255)

                       acaaaaaTTCAGCGCGATAAGGTTAAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTgggg                                                                 <  1:4636607/89‑1 (MQ=255)

                         acaAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTa                                                               >  2:1300295/1‑93 (MQ=255)

                         aaaaaTTCAGCGCTATGAGTTAGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTa                                                               <  1:447536/91‑1 (MQ=255)

                           aaaTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATg                                                             >  2:1489824/1‑93 (MQ=255)

                           aaaTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATg                                                             >  1:2829320/1‑93 (MQ=255)

                            aaTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGa                                                            >  1:4860584/1‑93 (MQ=255)

                               tCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAcgc                                                         >  1:825916/1‑93 (MQ=255)

                                 aGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCCCCGAATCTGGGGCATGACGCgg                                                       >  2:4924409/1‑93 (MQ=255)

                                      gATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGa                                                  <  1:2262006/93‑1 (MQ=255)

                                      gATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGa                                                  <  2:6107156/93‑1 (MQ=255)

                                       aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAt                                                 >  1:495607/1‑93 (MQ=255)

                                       aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAt                                                 <  1:5431237/93‑1 (MQ=255)

                                        tCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAtt                                                <  1:1400851/93‑1 (MQ=255)

                                        tCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAtt                                                >  2:795159/1‑93 (MQ=255)

                                        tCAGGTTAAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAtt                                                <  2:5644737/93‑1 (MQ=255)

                                         aaGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTc                                               <  2:5284384/92‑1 (MQ=255)

                                          aGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTca                                              >  1:6032909/1‑93 (MQ=255)

                                          aGGTTGAAGATTATGCCCGACGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTca                                              >  1:6550930/1‑93 (MQ=255)

                                           ggTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAGTCTGGGGTATGAAGCGGACTGATGcac                                             >  2:3827054/1‑93 (MQ=255)

                                           ggTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTcac                                             >  2:2756255/1‑93 (MQ=255)

                                           ggTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTcac                                             >  1:627513/1‑93 (MQ=255)

                                           ggTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTcac                                             >  1:6729268/1‑93 (MQ=255)

                                            gTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTcaca                                            >  1:1514206/1‑93 (MQ=255)

                                             ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACaa                                           >  2:2109581/1‑93 (MQ=255)

                                             ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACaa                                           >  2:714392/1‑93 (MQ=255)

                                             ttGAAAATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACaa                                           <  1:3812005/93‑1 (MQ=255)

                                              tGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACaaa                                          >  2:585627/1‑93 (MQ=255)

                                              tGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACaaa                                          >  2:4434105/1‑93 (MQ=255)

                                               gAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAAt                                         >  1:5151907/1‑93 (MQ=255)

                                                 aGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGCAGTTGGGCGCTGGCTGGCACCGAATCTGAGGACTGCCGCGACCTGATTCACAActct                                       >  2:6408148/1‑93 (MQ=255)

                                                 aGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCt                                       >  1:2877964/1‑93 (MQ=255)

                                                  gATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTg                                      >  1:3235560/1‑93 (MQ=255)

                                                   aTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGt                                     >  1:5811508/1‑93 (MQ=255)

                                                   aTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCTGt                                     >  2:4318982/1‑93 (MQ=255)

                                                    ttATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTc                                    <  1:2968054/93‑1 (MQ=255)

                                                     tATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCa                                   <  2:2071595/93‑1 (MQ=255)

                                                     tATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCa                                   <  2:6030245/93‑1 (MQ=255)

                                                      ttGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCAc                                  <  1:23503/92‑1 (MQ=255)

                                                        gCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACtt                                <  2:6458553/93‑1 (MQ=255)

                                                          ccgccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACtttt                              >  2:5215025/1‑93 (MQ=255)

                                                            gccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTcc                            <  1:2028279/93‑1 (MQ=255)

                                                             ccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCt                           <  1:1645339/93‑1 (MQ=255)

                                                               gTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTa                         >  1:1161947/1‑93 (MQ=255)

                                                               gTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTa                         <  1:6603351/93‑1 (MQ=255)

                                                                tAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTaa                        <  2:6239835/93‑2 (MQ=255)

                                                                 aaaGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAGTCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTaca                       >  2:1614673/1‑93 (MQ=255)

                                                                 aaaGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTaca                       >  2:1556777/1‑93 (MQ=255)

                                                                  aaGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTacaa                      >  2:1076339/1‑93 (MQ=255)

                                                                   aGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTacaac                     <  2:2002388/93‑1 (MQ=255)

                                                                     gTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTAcaacaa                   >  1:3464308/1‑93 (MQ=255)

                                                                      tATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaa                  >  2:5964181/1‑93 (MQ=255)

                                                                       aTGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaac                 <  2:316308/93‑1 (MQ=255)

                                                                       aTGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaac                 <  1:2195590/93‑1 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCGTTACaacaaaca                >  1:3852108/1‑93 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaaca                <  1:3623004/93‑1 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaaca                >  2:3909142/1‑93 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaaca                <  2:2213557/93‑1 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaaca                >  1:4504717/1‑93 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaaca                >  1:2617679/1‑93 (MQ=255)

                                                                         gAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAg               >  2:3652291/1‑93 (MQ=255)

                                                                           gCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAggg             <  1:874792/93‑1 (MQ=255)

                                                                            cGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGgc            <  2:575600/93‑1 (MQ=255)

                                                                            cGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGgc            <  1:977369/93‑1 (MQ=255)

                                                                              ttACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGgcgc          <  2:5714483/93‑1 (MQ=255)

                                                                              ttACCGAAGTGGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGgcgc          >  1:4071096/1‑93 (MQ=255)

                                                                               tACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGTGCGCt         >  2:2245188/1‑93 (MQ=255)

                                                                                aCCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTc        <  2:3355263/93‑1 (MQ=255)

                                                                                aCCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTc        >  1:5331910/1‑93 (MQ=255)

                                                                                 ccGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCa       <  1:6455846/93‑1 (MQ=255)

                                                                                  cGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa      >  2:4121556/1‑93 (MQ=255)

                                                                                   caaGTTGGGCGCTGGCTGTCACCGAATCTGGGGTATGACGCGGACTGATTCACAAAGCTGGCACTTTTCCTTACAACAAACAGGGCGCTCAAt     <  1:1822312/92‑1 (MQ=255)

                                                                                    aaGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATg    <  2:4209498/93‑1 (MQ=255)

                                                                                    aaGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATg    >  2:1834546/1‑93 (MQ=255)

                                                                                      gTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg  <  1:5765578/93‑1 (MQ=255)

                                                                                      gTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg  >  2:5834607/1‑93 (MQ=255)

                                                                                      gTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg  <  2:2977920/93‑1 (MQ=255)

CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAG  >  NC_000913/4224960‑4225138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTG  >  NC_000913/4224957‑4225140

CCCCGGACAGAAAGTACAACGGTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGG                                                                                            <  SRR4302536.501829/94‑1 (MQ=60)

ACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGG                                                                                            >  SRR4302536.1342688/1‑94 (MQ=60)

 CCCGGACAGCAAGTCCGCCGTTGTAGCAAAAATCCGGCGCTATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGG                                                                                           <  SRR4302536.4715700/94‑1 (MQ=60)

 CCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTACGCCCGCCGTAAAGGTATGAGCGTTACCGGAGTTGGG                                                                                           >  SRR4302536.6843586/1‑94 (MQ=60)

  cggaaaaataatttcttgggtctaccacaaattggggcgcacaAGGTTAAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGC                                                                                          <  SRR4302536.1307293/51‑1 (MQ=54)

  CCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGC                                                                                          >  SRR4302536.6330679/1‑94 (MQ=60)

   cggcaaccaatttctagGCTGTAACACAAATTCAGTGGGATCAGGTTAAATTTAATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                         <  SRR4302536.305900/77‑1 (MQ=36)

   CGGACAGAAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                         <  SRR4302536.4500229/94‑1 (MQ=60)

    GGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGC                                                                                        >  SRR4302536.5505985/1‑94 (MQ=60)

    GGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGC                                                                                        >  SRR4302536.3551892/1‑94 (MQ=60)

    GGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGC                                                                                        <  SRR4302536.3547977/94‑1 (MQ=60)

     GCCAGCAAGTACTGCCCTGTAGCACAATTTCAGCGCGATCAGGTTGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCT                                                                                       <  SRR4302536.4461564/94‑1 (MQ=60)

     GACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCT                                                                                       >  SRR4302536.2520848/1‑94 (MQ=60)

     GACAGAAAGTACTCCGCTGTAGCAAAAATTCAGCGCAATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCT                                                                                       <  SRR4302536.4280752/94‑1 (MQ=60)

      ACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAACGGTATGAGCGTTACCGAAGTTGGGCGCTG                                                                                      >  SRR4302536.4400536/1‑94 (MQ=60)

      AAAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCGGTTTAAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTG                                                                                      <  SRR4302536.1123987/94‑1 (MQ=60)

        AGGAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGCGCGCTGGC                                                                                    >  SRR4302536.6606233/1‑94 (MQ=60)

        AGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGC                                                                                    >  SRR4302536.3423173/1‑94 (MQ=60)

        AGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGC                                                                                    >  SRR4302536.1692173/1‑94 (MQ=60)

        AGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGC                                                                                    >  SRR4302536.1445964/1‑94 (MQ=60)

         cgcagtacaacgctgtacccaAAATTCAGCGCGATCAGGTTAAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCT                                                                                   <  SRR4302536.4558893/73‑1 (MQ=60)

         acaagtacaccgttgaAGCACAAATTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCT                                                                                   <  SRR4302536.481762/78‑1 (MQ=60)

          CAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTG                                                                                  >  SRR4302536.3361100/1‑94 (MQ=60)

           AAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGG                                                                                 >  SRR4302536.485857/1‑94 (MQ=60)

            AGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGC                                                                                >  SRR4302536.3215812/1‑94 (MQ=60)

            AGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCCAAGTTGGGCGCTGGCTGGC                                                                                >  SRR4302536.5585693/1‑94 (MQ=60)

                CTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCG                                                                            <  SRR4302536.5222976/94‑1 (MQ=60)

                 TACGCTGTAGCACAAATTCAGCGCGATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGA                                                                           <  SRR4302536.2783849/94‑1 (MQ=60)

                 TACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGA                                                                           >  SRR4302536.2925759/1‑94 (MQ=60)

                 TACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGA                                                                           >  SRR4302536.3721859/1‑94 (MQ=60)

                  ACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAA                                                                          >  SRR4302536.837624/1‑94 (MQ=60)

                   CGCTGTAGCACAAATTAAGCGCAATCAGGTTGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAAT                                                                         <  SRR4302536.6511788/94‑1 (MQ=60)

                    GCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATC                                                                        >  SRR4302536.3498931/1‑94 (MQ=60)

                    GCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATC                                                                        >  SRR4302536.1392815/1‑94 (MQ=60)

                     CTGTAGCCAAATTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCT                                                                       <  SRR4302536.4474657/94‑1 (MQ=60)

                      TGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                      >  SRR4302536.5172524/1‑94 (MQ=60)

                      TGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                      >  SRR4302536.49014/1‑94 (MQ=60)

                      TGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                      >  SRR4302536.4633324/1‑94 (MQ=60)

                      TGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                      >  SRR4302536.4648053/1‑94 (MQ=60)

                       GTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGG                                                                     >  SRR4302536.860543/1‑94 (MQ=60)

                       GTACCACAAATTCGGCGGGATCAGCTTGAGGATTCTCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGG                                                                     <  SRR4302536.3232768/94‑1 (MQ=60)

                        gagcaaaaatgcagcgcgatgggtTTGAAGATTGTGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGG                                                                    <  SRR4302536.4654827/70‑1 (MQ=60)

                        TAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGG                                                                    <  SRR4302536.6756377/94‑1 (MQ=60)

                         AGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGG                                                                   <  SRR4302536.4264567/94‑1 (MQ=60)

                         AGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGG                                                                   >  SRR4302536.3914611/1‑94 (MQ=60)

                         AGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGG                                                                   >  SRR4302536.2501199/1‑94 (MQ=60)

                          acaaAAATTCAGCGCGATAAGGTTAAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGT                                                                  <  SRR4302536.4775951/90‑1 (MQ=60)

                            AAAAATTCAGCGCTATGAGTTAGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTAT                                                                <  SRR4302536.459299/94‑1 (MQ=60)

                             CAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATG                                                               >  SRR4302536.2913087/1‑94 (MQ=60)

                              AAATTCAGCTCGATCAGGTTGGAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGA                                                              >  SRR4302536.6763353/1‑94 (MQ=60)

                              AAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGA                                                              >  SRR4302536.5006858/1‑94 (MQ=60)

                              AAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGA                                                              >  SRR4302536.2043873/1‑94 (MQ=60)

                                atccgGCGCGATCAGGTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACG                                                            <  SRR4302536.6307063/89‑1 (MQ=60)

                                 TTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGC                                                           >  SRR4302536.850234/1‑94 (MQ=60)

                                   aggcgaagtcaggtttaaaattagtCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGG                                                         <  SRR4302536.1303453/69‑1 (MQ=60)

                                    AGCGCTATCAGGTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGA                                                        <  SRR4302536.2532320/94‑1 (MQ=60)

                                    AGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGA                                                        >  SRR4302536.868912/1‑94 (MQ=60)

                                      cgagagcaggtaaaagattgtcCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACT                                                      <  SRR4302536.3515391/72‑1 (MQ=60)

                                      CGCGATCGGGTGGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACT                                                      <  SRR4302536.4234726/94‑1 (MQ=60)

                                      CGCGATCAGGTTGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACT                                                      <  SRR4302536.609335/94‑1 (MQ=60)

                                      CGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACT                                                      >  SRR4302536.5822438/1‑94 (MQ=60)

                                      CGCGATAAGGTGGAAGATTAGGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACT                                                      <  SRR4302536.6564267/94‑1 (MQ=60)

                                        CGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGA                                                    >  SRR4302536.1601196/1‑94 (MQ=60)

                                         GATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                   <  SRR4302536.2330518/94‑1 (MQ=60)

                                         GATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                   >  SRR4302536.508400/1‑94 (MQ=60)

                                         GATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                   >  SRR4302536.4579263/1‑94 (MQ=60)

                                         GATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                   >  SRR4302536.2481450/1‑94 (MQ=60)

                                         GATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                   >  SRR4302536.329288/1‑94 (MQ=60)

                                          ATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATT                                                  <  SRR4302536.5593632/94‑1 (MQ=60)

                                           TCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTC                                                 >  SRR4302536.5013889/1‑94 (MQ=60)

                                           TCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTC                                                 <  SRR4302536.1442519/94‑1 (MQ=60)

                                            CAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCA                                                >  SRR4302536.6212805/1‑94 (MQ=60)

                                            CAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCA                                                >  SRR4302536.617649/1‑94 (MQ=60)

                                            CAGGTTGAAGATTATGCCCGACGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCA                                                >  SRR4302536.6746005/1‑94 (MQ=60)

                                             AGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCAC                                               >  SRR4302536.6929252/1‑94 (MQ=60)

                                             AGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCAC                                               >  SRR4302536.646114/1‑94 (MQ=60)

                                              GGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACA                                              >  SRR4302536.1558793/1‑94 (MQ=60)

                                                TTGAAAATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAA                                            <  SRR4302536.3925646/94‑1 (MQ=60)

                                                TGGAAGATAATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAA                                            <  SRR4302536.3456614/94‑1 (MQ=60)

                                                 TGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAAT                                           >  SRR4302536.1176618/1‑94 (MQ=60)

                                                 TGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAAT                                           >  SRR4302536.5305616/1‑94 (MQ=60)

                                                  GAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATC                                          >  SRR4302536.2107501/1‑94 (MQ=60)

                                                   AAGTTTACGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCT                                         <  SRR4302536.6244502/94‑1 (MQ=60)

                                                   AAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCT                                         <  SRR4302536.96998/94‑1 (MQ=60)

                                                   AAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCT                                         >  SRR4302536.2962909/1‑94 (MQ=60)

                                                   AAGATTACGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCT                                         <  SRR4302536.4171781/94‑1 (MQ=60)

                                                    AGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTG                                        >  SRR4302536.3330405/1‑94 (MQ=60)

                                                     GATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGT                                       >  SRR4302536.5984615/1‑94 (MQ=60)

                                                      ATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTC                                      >  SRR4302536.5914257/1‑94 (MQ=60)

                                                      ATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTC                                      >  SRR4302536.37366/1‑94 (MQ=60)

                                                       TTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCA                                     <  SRR4302536.3055479/94‑1 (MQ=60)

                                                       TTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGCCA                                     <  SRR4302536.318280/94‑1 (MQ=60)

                                                        TATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCAC                                    >  SRR4302536.648932/1‑94 (MQ=60)

                                                         TTGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACT                                   <  SRR4302536.24159/94‑1 (MQ=60)

                                                         ATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACT                                   <  SRR4302536.3943467/94‑1 (MQ=60)

                                                           GCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTAATTCACAAATATGTCACTTT                                 <  SRR4302536.6711888/94‑1 (MQ=60)

                                                            CCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTT                                <  SRR4302536.2328879/94‑1 (MQ=60)

                                                             CCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTC                               <  SRR4302536.5080377/94‑1 (MQ=60)

                                                               GCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCT                             <  SRR4302536.2089344/94‑1 (MQ=60)

                                                                CCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCAGTTTTCCTT                            >  SRR4302536.4068835/1‑94 (MQ=60)

                                                                CCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTT                            >  SRR4302536.2182475/1‑94 (MQ=60)

                                                                CCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTT                            <  SRR4302536.4246689/94‑1 (MQ=60)

                                                                CCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTT                            <  SRR4302536.1693188/94‑1 (MQ=60)

                                                                CCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTT                            <  SRR4302536.1534385/94‑1 (MQ=60)

                                                                 CGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTA                           >  SRR4302536.1196924/1‑94 (MQ=60)

                                                                  GTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTAC                          <  SRR4302536.6799739/94‑1 (MQ=60)

                                                                   TAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACA                         <  SRR4302536.2410504/94‑1 (MQ=60)

                                                                    AAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAA                        <  SRR4302536.1723091/94‑1 (MQ=60)

                                                                    AAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAA                        <  SRR4302536.3495567/94‑1 (MQ=60)

                                                                     AAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAAC                       >  SRR4302536.5983571/1‑94 (MQ=60)

                                                                       GGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAA                     >  SRR4302536.3567549/1‑94 (MQ=60)

                                                                        GTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAA                    <  SRR4302536.3056627/94‑1 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCGTTACAACAAACA                  >  SRR4302536.3966779/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                  >  SRR4302536.6691005/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                  >  SRR4302536.6614677/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                  <  SRR4302536.1352135/94‑1 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                  >  SRR4302536.2696212/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                  >  SRR4302536.4639891/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                  <  SRR4302536.2261861/94‑1 (MQ=60)

                                                                           TGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAG                 <  SRR4302536.3731333/94‑1 (MQ=60)

                                                                           TGAGCGTTACCGAAGTTGGGCGCTGGCTAGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAG                 <  SRR4302536.2011860/94‑1 (MQ=60)

                                                                            GAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGG                <  SRR4302536.2888366/94‑1 (MQ=60)

                                                                              GCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGC              <  SRR4302536.900569/94‑1 (MQ=60)

                                                                              GCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGC              >  SRR4302536.5415150/1‑94 (MQ=60)

                                                                               CGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCG             <  SRR4302536.1006211/94‑1 (MQ=60)

                                                                               CGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCG             <  SRR4302536.482968/94‑1 (MQ=60)

                                                                               CGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCG             >  SRR4302536.3015909/1‑94 (MQ=60)

                                                                                GTTACCGAAGTGGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGC            >  SRR4302536.4191748/1‑94 (MQ=60)

                                                                                 TTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCCGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCC           >  SRR4302536.2943113/1‑94 (MQ=60)

                                                                                  TACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTC          >  SRR4302536.5491217/1‑94 (MQ=60)

                                                                                  TACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGAGCGCTC          >  SRR4302536.1438231/1‑94 (MQ=60)

                                                                                    CCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAA        <  SRR4302536.6648628/94‑1 (MQ=60)

                                                                                    CCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAA        <  SRR4302536.6542311/94‑1 (MQ=60)

                                                                                      GAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATG      <  SRR4302536.2388654/94‑1 (MQ=60)

                                                                                      CAAGTTGGGCGCTGGCTGTCACCGAATCTGGGGTATGACGCGGACTGATTCACAAAGCTGGCACTTTTCCTTACAACAAACAGGGCGCTCAATG      <  SRR4302536.1877283/94‑1 (MQ=60)

                                                                                       AAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGA     <  SRR4302536.751258/94‑1 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC   <  SRR4302536.1357853/94‑1 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC   <  SRR4302536.5937113/94‑1 (MQ=60)

                                                                                          TTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCG  <  SRR4302536.6835488/94‑1 (MQ=60)

ACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTG  >  NC_000913/4224957‑4225140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`