Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,225,139 T→C intergenic (+49/‑171) metH → / → yjbB B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase/putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,225,1390TC99.4% 527.4 / NA 168intergenic (+49/‑171)metH/yjbBB12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase/putative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (97/70);  minor base A (1/0);  total (98/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAG  >  NC_000913/4225049‑4225226
                                                                                          |                                                                                        
gggcgcTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAgcgc                                                                                        >  2:1559241/3‑93 (MQ=255)
gggcgcTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCGTACAACAAACAGGGCGCTCAATGAgcgc                                                                                        >  2:6445527/3‑93 (MQ=255)
     ctggctggCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGt                                                                                   >  2:3298523/1‑93 (MQ=255)
      tggctggcACCGAATCTGGGGTAGGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtc                                                                                  <  2:5091990/93‑1 (MQ=255)
       ggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtct                                                                                 >  1:2877446/1‑93 (MQ=255)
          tggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCtt                                                                              >  1:4160024/1‑93 (MQ=255)
           ggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGTGTGCTCAATGAGCTACCTCTCTCttt                                                                             >  1:4857454/1‑93 (MQ=255)
              cccGAATCTGGGGTATGTCGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatt                                                                          <  2:2091556/92‑1 (MQ=255)
              aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCtttttt                                                                          >  1:1676451/1‑93 (MQ=255)
              aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatt                                                                          >  1:6427279/1‑93 (MQ=255)
              aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatt                                                                          >  1:3369096/1‑93 (MQ=255)
               ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatta                                                                         <  1:861534/93‑1 (MQ=255)
                 gAATCGGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAat                                                                       <  1:6367356/93‑1 (MQ=255)
                      tGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGaa                                                                  >  1:5901182/1‑93 (MQ=255)
                      tGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGaa                                                                  >  1:6574036/1‑93 (MQ=255)
                      tGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGaa                                                                  >  1:621526/1‑93 (MQ=255)
                       ggggTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGaaa                                                                 >  1:2049119/1‑93 (MQ=255)
                        gggTATGACGCGGACTGATTCACAAATCTGTCATTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAac                                                                <  1:3115023/93‑1 (MQ=255)
                        gggTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAac                                                                <  1:4483038/93‑1 (MQ=255)
                          gTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAacac                                                              >  2:6664101/1‑93 (MQ=255)
                           taccaCGCGGCGTGATTCACAAATCTGTCACTTTTCATTACAACACACAGGCCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑t                                                            <  2:307836/89‑1 (MQ=255)
                             tGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCCTTATTAATATGAAAAAA‑Tta                                                          >  1:2455944/1‑93 (MQ=255)
                             tGACGCGGACTGATTCACAAATCTGTCACTTTCCCTTACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑Tta                                                          <  2:3858635/93‑1 (MQ=255)
                               aCGCGGACTGATCCACAAATCTGTCACTTTTCCTTACAACAAACGGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑Ttata                                                        <  1:6092098/93‑1 (MQ=255)
                                cgcgGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATAc                                                       >  1:5101192/1‑93 (MQ=255)
                                 gcgGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACt                                                      >  2:2649726/1‑93 (MQ=255)
                                  gggACTTATTCACAACTTTTTCTTTTTTCCTAAAAAAAAACGGGCCCCTAAAGGCCCCCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTg                                                     <  1:3691856/92‑1 (MQ=255)
                                  cgGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGGGCCATGAGCGCCCCCTTTCTTTTTTAATATTAAAAAA‑ATATACTg                                                     >  2:3581482/1‑93 (MQ=255)
                                   ggACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTgg                                                    >  2:5303180/1‑93 (MQ=255)
                                    gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGCGCCCCGTCTTTTTTTTTATATGAAAAAC‑TTATTCTTGa                                                   >  1:3574857/1‑93 (MQ=255)
                                    gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATTAAACAC‑TTATACTGGa                                                   >  2:2549414/1‑93 (MQ=255)
                                     aCTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTATTAATATGAAAAAC‑TTATACTTGaa                                                  >  1:1335191/1‑93 (MQ=255)
                                      cTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGaaa                                                 <  2:3991885/93‑1 (MQ=255)
                                       tGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAAc                                                >  2:4425307/1‑93 (MQ=255)
                                       tGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAAc                                                >  2:4299732/1‑93 (MQ=255)
                                       tGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAAc                                                >  1:6717826/1‑93 (MQ=255)
                                        gATTCACAAATCTGTCTCTTTTCCTAACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACa                                               <  2:5750599/93‑1 (MQ=255)
                                        gATTCACAAATCGTTCACTTTTCCTTACAACAAACAGGGCGTTCATTGACCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACa                                               <  2:6277845/93‑1 (MQ=255)
                                         aTTCACAAATCTGTCATTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAg                                              <  2:4162160/93‑1 (MQ=255)
                                         aTTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAg                                              <  1:2964095/93‑1 (MQ=255)
                                         aTTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAg                                              >  1:490197/1‑93 (MQ=255)
                                           tCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGc                                            >  2:6123211/1‑93 (MQ=255)
                                             acaAGTCTGTCACTTTTCCTTACACAAAACAGGGCTCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTg                                          <  1:4108107/93‑1 (MQ=255)
                                             acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAAAAAGCTg                                          >  1:640000/1‑93 (MQ=255)
                                             acaAATCTGTCACTTGCCCTTACAACAAGCAGGGCGTCCAAGGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTg                                          <  2:1944979/93‑1 (MQ=255)
                                              caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTgg                                         >  1:460917/1‑93 (MQ=255)
                                              caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTgg                                         <  1:1919086/93‑1 (MQ=255)
                                              caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTgg                                         >  2:2203663/1‑93 (MQ=255)
                                               aaaTCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGa                                        >  1:5318925/1‑93 (MQ=255)
                                               aaaTCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGAGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGa                                        >  2:2979137/1‑93 (MQ=255)
                                                aaTCTGTCACTTTTCCTTACAACAAACGGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGaa                                       <  2:1357200/93‑1 (MQ=255)
                                                   ctctCATTTTTCCTCACACAAACCGGGGCGCTATATGAGCACCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGaataa                                    <  2:579619/90‑1 (MQ=255)
                                                    tGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGgataat                                   >  2:6273139/1‑93 (MQ=255)
                                                    tGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGACCCCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGaataat                                   <  2:4860584/93‑1 (MQ=255)
                                                     gTCTCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATc                                  <  1:5077758/93‑1 (MQ=255)
                                                      tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCt                                 <  1:1883635/93‑1 (MQ=255)
                                                       taCTTTTCCGTACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCtt                                <  1:2012722/92‑1 (MQ=255)
                                                       cACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCtt                                <  1:2219181/93‑1 (MQ=255)
                                                        aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTTGAATAATCTTa                               >  2:380999/1‑93 (MQ=255)
                                                        aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTa                               >  2:4474294/1‑93 (MQ=255)
                                                        aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTa                               <  2:6729268/93‑1 (MQ=255)
                                                        aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTa                               <  1:898615/93‑1 (MQ=255)
                                                         cTTTTCCTTACAACAAACAGGGGGGTCCATGAAGGCCCCGTCTCTTTTTTTATATTAAAAACATTTAATTGAAAAAAGGCGGGAATAATTTTa                               >  2:4660900/1‑93 (MQ=255)
                                                          ttttCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGc                             >  1:2516993/1‑93 (MQ=255)
                                                          ttttCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGc                             >  2:5549863/1‑93 (MQ=255)
                                                          ttttCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGc                             >  2:3385880/1‑93 (MQ=255)
                                                           tttCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGcc                            >  1:2907420/1‑93 (MQ=255)
                                                            ttCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCg                           >  1:3044218/1‑93 (MQ=255)
                                                            taccTTACAACAAACAGGGCCCTAAATGAGACCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCg                           <  2:3925058/91‑1 (MQ=255)
                                                               cTTACAACCAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                        >  1:6543179/1‑93 (MQ=255)
                                                               cTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                        <  2:1773778/93‑1 (MQ=255)
                                                               cTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                        >  2:5465961/1‑93 (MQ=255)
                                                               cTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                        >  1:5530649/1‑93 (MQ=255)
                                                                 tACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaa                      >  2:4089833/1‑93 (MQ=255)
                                                                  acaacaAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaaa                     <  2:2222320/93‑1 (MQ=255)
                                                                    aacaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGGCGGAAAAACg                   >  2:6082023/1‑93 (MQ=255)
                                                                    aacaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACg                   >  1:958850/1‑93 (MQ=255)
                                                                    aacaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACg                   >  1:5421107/1‑93 (MQ=255)
                                                                     acaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGGATAATCTTAGACGGAAAAAAGa                  >  1:3829208/1‑93 (MQ=255)
                                                                     acaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                  >  2:5587519/1‑93 (MQ=255)
                                                                      caaacaGGGCGTTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGaa                 <  1:275681/93‑1 (MQ=255)
                                                                      caaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGGATAATCTTAGCCGGAAAAACGaa                 >  2:3319587/1‑93 (MQ=255)
                                                                      caaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGaa                 <  1:706869/93‑1 (MQ=255)
                                                                       aaacgGGGCGCACAAGGAGCCCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAt                <  1:4711553/93‑1 (MQ=255)
                                                                        aacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATg               >  2:2605806/1‑93 (MQ=255)
                                                                        aacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATg               >  1:5571170/1‑93 (MQ=255)
                                                                         acggggCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt              <  2:3654731/90‑1 (MQ=255)
                                                                         acaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGGATAATCTTAGCCCGAAAAACGAAAGt              >  2:201673/1‑93 (MQ=255)
                                                                         acaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt              >  2:1105991/1‑93 (MQ=255)
                                                                              gcgcTCAAGGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaaa         <  2:600311/93‑1 (MQ=255)
                                                                               cgcTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAg        >  2:6223546/1‑93 (MQ=255)
                                                                               cgcTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAg        <  2:6251019/93‑1 (MQ=255)
                                                                                gcTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAgg       >  2:150168/1‑93 (MQ=255)
                                                                                 cTCAATGAGCTCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGc      <  1:6408148/93‑1 (MQ=255)
                                                                                 cTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGc      >  1:1004756/1‑93 (MQ=255)
                                                                                  taaaTGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGca     <  2:4917644/91‑1 (MQ=255)
                                                                                  tCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGca     >  1:4023941/1‑93 (MQ=255)
                                                                                  tCAATGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGca     >  1:3152986/1‑93 (MQ=255)
                                                                                    aaTGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca   >  2:3163508/1‑93 (MQ=255)
                                                                                     aTGAGCGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAg  <  1:4826804/93‑1 (MQ=255)
                                                                                          |                                                                                        
GAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACAC‑TTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAG  >  NC_000913/4225049‑4225226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  NC_000913/4225046‑4225232
                                                                                             |                                                                                             
GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               <  SRR4302536.1357853/94‑1 (MQ=60)
GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               <  SRR4302536.5937113/94‑1 (MQ=60)
 TTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCG                                                                                              <  SRR4302536.6835488/94‑1 (MQ=60)
     GCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCT                                                                                          <  SRR4302536.2198876/94‑1 (MQ=60)
      CGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTG                                                                                         <  SRR4302536.888390/94‑1 (MQ=60)
       GCTGGCTGGCACCGAATCGGGGGTATGACGCGGACTGATTCACAAATCTGACATTTTTCCTTACAAAAAACATGGCGCAAAATGAGCCCCCTGT                                                                                        <  SRR4302536.5146917/94‑1 (MQ=60)
         TGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCT                                                                                      >  SRR4302536.2962378/1‑94 (MQ=60)
         TGGCGGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCATTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCT                                                                                      <  SRR4302536.661336/94‑1 (MQ=60)
            CTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAACCAGGGCGCTCAATGAGCGCCCTGTCTCTT                                                                                   <  SRR4302536.3042841/94‑1 (MQ=60)
            CTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTT                                                                                   >  SRR4302536.4283542/1‑94 (MQ=60)
             TGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACATTCAGGGCGCTCAATGAGCGCCCTGTCTCTTT                                                                                  >  SRR4302536.6921110/1‑94 (MQ=60)
             TGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTT                                                                                  <  SRR4302536.6585053/94‑1 (MQ=60)
             TGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTT                                                                                  <  SRR4302536.4247092/94‑1 (MQ=60)
                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTTTT                                                                               >  SRR4302536.1725060/1‑94 (MQ=60)
                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATT                                                                               >  SRR4302536.6619364/1‑94 (MQ=60)
                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATT                                                                               >  SRR4302536.3469183/1‑94 (MQ=60)
                  CTGAATCTGGGGTATGACGCGGCCTATTTCACAAATCTGTCCCTTTTCCTAAAAAAAACCAGGGCGCTCATTGAGCTCCCTGTCTCTTTATTAA                                                                             <  SRR4302536.5200062/94‑1 (MQ=28)
                  CCGAATCTGGGTTAGGACGCGGCCTGATTCACAAATCTGTCATTTTCCCTTACAACAAACAGGGCGCTAAAGGAGCGCCCTGTCTCTTTATTAA                                                                             <  SRR4302536.528316/94‑1 (MQ=60)
                  CCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAA                                                                             <  SRR4302536.886861/94‑1 (MQ=60)
                   CGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAAT                                                                            >  SRR4302536.373736/1‑94 (MQ=60)
                   CGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAAT                                                                            <  SRR4302536.5612339/94‑1 (MQ=60)
                    GAATCGGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATA                                                                           <  SRR4302536.6557096/94‑1 (MQ=60)
                     AATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTTTTTATAT                                                                          >  SRR4302536.4944650/1‑94 (MQ=60)
                     AATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCACTGTCTCTTTATTAATAT                                                                          >  SRR4302536.2897191/1‑94 (MQ=60)
                      atcgggggattgaggcggacgaATTCAAAAATCTGTCATTTTCCCTTACAACAAACGGGGCGCTCAATGAGCCCCCTGTCTCTTTATTAATATG                                                                         <  SRR4302536.2005647/72‑1 (MQ=48)
                        CTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAA                                                                       >  SRR4302536.639986/1‑94 (MQ=60)
                        CTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAA                                                                       >  SRR4302536.6769681/1‑94 (MQ=60)
                        CTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAA                                                                       >  SRR4302536.6076920/1‑94 (MQ=60)
                         TGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGTCGCTCAATGAGCGCCCTGTCTCTTTCTTAATATGAAA                                                                      >  SRR4302536.3023792/1‑94 (MQ=60)
                         TGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAA                                                                      <  SRR4302536.1948090/94‑1 (MQ=60)
                         TGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAA                                                                      >  SRR4302536.2110823/1‑94 (MQ=60)
                           GGGTATGACGCGGACTGATTCACAAATCTGTCATTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACA                                                                    <  SRR4302536.3206006/94‑1 (MQ=60)
                           GGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACA                                                                    <  SRR4302536.848018/94‑1 (MQ=60)
                           GGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACA                                                                    <  SRR4302536.4617683/94‑1 (MQ=60)
                              TATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGGTTCTTTATTAATATTAAACACTT                                                                 >  SRR4302536.548161/1‑94 (MQ=60)
                               ATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGATCGCCCTGTCTCTTTATTAATATGAAACACTTA                                                                >  SRR4302536.4202082/1‑94 (MQ=60)
                               ATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCCTTATTAATATGAAAAAATTA                                                                >  SRR4302536.2530098/1‑94 (MQ=60)
                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             >  SRR4302536.5253712/1‑94 (MQ=60)
                                  ACGCGGACTGATCCACAAATCTGTCACTTTTCCTTACAACAAACGGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302536.6273768/94‑1 (MQ=60)
                                   cgcgacttgatccaaAAATCTGTCATTTTTCCTTACAAAAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACT                                                            <  SRR4302536.5091433/79‑1 (MQ=60)
                                   CGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACT                                                            <  SRR4302536.925936/94‑1 (MQ=60)
                                   CGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTTTTAATATTAAACACTTATACT                                                            >  SRR4302536.2477490/1‑94 (MQ=60)
                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTTTTTATATGAAACACTTATACTTG                                                          >  SRR4302536.1403689/1‑94 (MQ=60)
                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTTATATTAAACACCTATACTTG                                                          >  SRR4302536.4766290/1‑94 (MQ=60)
                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGCGCCCCGTCTTTTTTTTTATATGAAAAACTTATTCTTGA                                                         >  SRR4302536.3681740/1‑94 (MQ=60)
                                       GACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTATTAATATGAAAAACTTATACTTGAA                                                        >  SRR4302536.1375033/1‑94 (MQ=60)
                                        ACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAA                                                       <  SRR4302536.4605005/94‑1 (MQ=60)
                                        ACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAA                                                       >  SRR4302536.6748157/1‑94 (MQ=60)
                                         CTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAC                                                      >  SRR4302536.6917262/1‑94 (MQ=60)
                                         CTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTCGAAAC                                                      >  SRR4302536.3096111/1‑94 (MQ=60)
                                           GATTCACAAATCTGTCACTTTTCTTTACAACAAACAGGGCGCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    <  SRR4302536.500615/94‑1 (MQ=60)
                                           GATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    >  SRR4302536.502891/1‑94 (MQ=60)
                                           GATTCACAAATCTATCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    <  SRR4302536.2097940/94‑1 (MQ=60)
                                           GATCCACAAATCTGTCTTTTTTCCTTACAACAAACGGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    <  SRR4302536.321565/94‑1 (MQ=60)
                                            ATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGCGCCCTCTCTCTTTATTAATATTAAACACTTCTACTGGAAACAGG                                                   >  SRR4302536.3162053/1‑94 (MQ=60)
                                            ATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGG                                                   <  SRR4302536.3051402/94‑1 (MQ=60)
                                              tcaaaaatctctcttTTTTCCTAACAACAAACGGGGCGCTAAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCT                                                 <  SRR4302536.4992562/79‑1 (MQ=60)
                                              TCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCT                                                 >  SRR4302536.2992373/1‑94 (MQ=60)
                                               CACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTG                                                <  SRR4302536.5697099/94‑1 (MQ=60)
                                               CACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAAAAGCTG                                                >  SRR4302536.658886/1‑94 (MQ=60)
                                               CACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCGCCATGAGCGCCCTCTCTCTTTTTTTATATTCAACACTTATACTtggaaaaagctg                                                >  SRR4302536.3596766/1‑82 (MQ=43)
                                               CACAAATCTGTCACTTTTCCTTACAACAAACAAGGCGCTCAATGAGCGCCCTGTCTCTTTTTTAATATTAAAAACTTATACTTGAAAAAAGCTG                                                >  SRR4302536.6862161/1‑94 (MQ=60)
                                                ACAAGTCTGTCACTTTTCCTTACACAAAACAGGGCTCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGG                                               <  SRR4302536.4229978/94‑1 (MQ=60)
                                                ACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGG                                               >  SRR4302536.472959/1‑94 (MQ=60)
                                                 CAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGA                                              >  SRR4302536.5477817/1‑94 (MQ=60)
                                                 CAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGA                                              <  SRR4302536.1976826/94‑1 (MQ=60)
                                                  AAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGGA                                             >  SRR4302536.6303976/1‑94 (MQ=60)
                                                     TCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAA                                          >  SRR4302536.6714916/1‑94 (MQ=60)
                                                     TCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAA                                          <  SRR4302536.4448289/94‑1 (MQ=60)
                                                      CTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGAGCCCCGTCTCTTTATTAATATGAAACACTTATACTGGAAACAAGCTGGGATAAT                                         >  SRR4302536.1480378/1‑94 (MQ=60)
                                                       TGTCACTTTTCCTTACAACAAACAGGGGGCTCAATGAGAGCCCCGTCTCTTTttttatattaaaaaaatatacttggaacacgctgggataaat                                        >  SRR4302536.4781956/1‑52 (MQ=49)
                                                        ttcttTTTTCCTAACAACAAGCGGGGCGCTCAATGACCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCT                                       <  SRR4302536.3644258/89‑1 (MQ=60)
                                                        GTCTCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCT                                       <  SRR4302536.5229729/94‑1 (MQ=60)
                                                         TCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAttaacttt                                      >  SRR4302536.3219080/1‑86 (MQ=60)
                                                         TCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTT                                      <  SRR4302536.1940308/94‑1 (MQ=60)
                                                          TACTTTTCCGTACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTA                                     <  SRR4302536.2073290/94‑1 (MQ=60)
                                                          CACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTA                                     <  SRR4302536.2286298/94‑1 (MQ=60)
                                                           ACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAG                                    <  SRR4302536.925090/94‑1 (MQ=60)
                                                            tttttcctcaaaacaaggggggctCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGC                                   <  SRR4302536.2551165/70‑1 (MQ=60)
                                                            CTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGC                                   >  SRR4302536.2592884/1‑94 (MQ=60)
                                                             TTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCC                                  >  SRR4302536.2993235/1‑94 (MQ=60)
                                                              TTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCG                                 >  SRR4302536.3133690/1‑94 (MQ=60)
                                                              TTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCG                                 >  SRR4302536.3964146/1‑94 (MQ=60)
                                                                TCCTTACAACAAACAGGGCTCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGA                               <  SRR4302536.6444362/94‑1 (MQ=60)
                                                                 CCTTTCAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAA                              >  SRR4302536.2308373/1‑94 (MQ=60)
                                                                 CCTTACAACCAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAA                              >  SRR4302536.6738032/1‑94 (MQ=60)
                                                                 CCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAA                              >  SRR4302536.5695526/1‑94 (MQ=60)
                                                                  CTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAA                             >  SRR4302536.5283094/1‑94 (MQ=60)
                                                                  CTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTATTTATATTAAACACCTATACTGGAAAAAAGCTGGGATAATCTTTTCCGGAAA                             >  SRR4302536.5070584/1‑94 (MQ=44)
                                                                     ACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAA                          >  SRR4302536.513902/1‑94 (MQ=60)
                                                                      CAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACG                         >  SRR4302536.987193/1‑94 (MQ=60)
                                                                      CAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACG                         >  SRR4302536.5583157/1‑94 (MQ=60)
                                                                       AACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGGATAATCTTAGACGGAAAAAAGA                        >  SRR4302536.3943267/1‑94 (MQ=60)
                                                                         CAAACAGGGCGTTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302536.283015/94‑1 (MQ=60)
                                                                         CAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302536.727534/94‑1 (MQ=60)
                                                                         CAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302536.2055744/94‑1 (MQ=60)
                                                                          AAACGGGGCGCACAAGGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     <  SRR4302536.4853274/94‑1 (MQ=60)
                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGGATAATCTTTGCCGGAAAAACGAATG                     >  SRR4302536.5238824/1‑94 (MQ=60)
                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     >  SRR4302536.5737021/1‑94 (MQ=60)
                                                                           AACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGT                    >  SRR4302536.1782457/1‑94 (MQ=60)
                                                                             CAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCA                  <  SRR4302536.6432744/94‑1 (MQ=60)
                                                                              AGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAA                 >  SRR4302536.5232237/1‑94 (MQ=60)
                                                                               GGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAA                <  SRR4302536.2779312/94‑1 (MQ=60)
                                                                                 GCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAG              <  SRR4302536.746160/94‑1 (MQ=60)
                                                                                   GCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGC            >  SRR4302536.1034407/1‑94 (MQ=60)
                                                                                    CTCAATGAGCTCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           <  SRR4302536.6599547/94‑1 (MQ=60)
                                                                                    CTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           <  SRR4302536.6599546/94‑1 (MQ=60)
                                                                                    CTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           >  SRR4302536.3245321/1‑94 (MQ=60)
                                                                                    CTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           >  SRR4302536.3364559/1‑94 (MQ=60)
                                                                                    CTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           >  SRR4302536.4143317/1‑94 (MQ=60)
                                                                                    CTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           >  SRR4302536.4652637/1‑94 (MQ=60)
                                                                                      CAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACA         >  SRR4302536.3202243/1‑94 (MQ=60)
                                                                                       AATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAG        >  SRR4302536.5958708/1‑94 (MQ=60)
                                                                                        ATGAGCTCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGT       <  SRR4302536.6246652/94‑1 (MQ=60)
                                                                                        ATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGT       <  SRR4302536.4972072/94‑1 (MQ=60)
                                                                                             CGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  SRR4302536.4783590/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  NC_000913/4225046‑4225232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑