Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,259,162 A→G intergenic (+277/‑86) yjbM → / → yjbN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,259,1620AG99.6% 889.1 / NA 267intergenic (+277/‑86)yjbM/yjbNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (136/130);  minor base C (1/0);  total (137/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC  >  NC_000913/4259072‑4259251
                                                                                          |                                                                                         
 tattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTAtggtatggta                                                                                        >  2:2079362/1‑93 (MQ=255)
  attTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAc                                                                                       >  2:2487956/1‑93 (MQ=255)
       cATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGc                                                                                  >  1:1432770/1‑93 (MQ=255)
         tCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTa                                                                                <  1:4573021/93‑1 (MQ=255)
          cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAt                                                                               <  2:3760217/93‑1 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              <  1:3801536/93‑1 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              >  2:4051725/1‑93 (MQ=255)
             ttCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGa                                                                            >  2:1985045/1‑93 (MQ=255)
               cGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGc                                                                          <  1:6670633/93‑1 (MQ=255)
               cGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGc                                                                          >  2:1329477/1‑93 (MQ=255)
                gTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcc                                                                         <  2:4530516/93‑1 (MQ=255)
                 tGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcct                                                                        >  1:442191/1‑93 (MQ=255)
                 tGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcct                                                                        >  1:5142474/1‑93 (MQ=255)
                 tGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcct                                                                        >  1:3291602/1‑93 (MQ=255)
                   agagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcc                                                                      >  1:896337/1‑93 (MQ=255)
                   agagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcc                                                                      <  2:3181360/93‑1 (MQ=255)
                   agagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcc                                                                      >  2:4504894/1‑93 (MQ=255)
                    gagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcct                                                                     <  1:1982829/93‑1 (MQ=255)
                    gagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcct                                                                     <  2:6205126/93‑1 (MQ=255)
                    aagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcct                                                                     <  1:6425877/92‑1 (MQ=255)
                     agCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTg                                                                    <  1:3086949/93‑1 (MQ=255)
                     agCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTg                                                                    >  1:5677883/1‑93 (MQ=255)
                      gCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGa                                                                   >  1:1146738/1‑93 (MQ=255)
                       cTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGaa                                                                  <  2:4334084/93‑1 (MQ=255)
                       cTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGaa                                                                  <  1:4285609/93‑1 (MQ=255)
                        ttCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAt                                                                 >  1:587490/1‑93 (MQ=255)
                        ttCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAt                                                                 <  1:4133333/93‑1 (MQ=255)
                        ttCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAt                                                                 <  1:3587593/93‑1 (MQ=255)
                          cATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAg                                                               <  2:3922337/93‑1 (MQ=255)
                             gCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGa                                                            >  2:6061313/1‑93 (MQ=255)
                             gCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGa                                                            >  2:961872/1‑93 (MQ=255)
                              ccTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAt                                                           <  1:5552938/93‑1 (MQ=255)
                                ttGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGc                                                         <  1:1908331/93‑1 (MQ=255)
                                ttGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGc                                                         <  2:861040/93‑1 (MQ=255)
                                   agagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGa                                                      >  2:3276210/1‑93 (MQ=255)
                                   agagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGa                                                      <  1:4766452/93‑1 (MQ=255)
                                    gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGaa                                                     >  2:1735905/1‑93 (MQ=255)
                                    gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGaa                                                     >  1:6528041/1‑93 (MQ=255)
                                    gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGaa                                                     >  1:120310/1‑93 (MQ=255)
                                    gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGaa                                                     >  1:2668569/1‑93 (MQ=255)
                                     agGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAat                                                    >  2:379654/1‑93 (MQ=255)
                                     agGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAat                                                    <  1:5393981/93‑1 (MQ=255)
                                      ggATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAata                                                   >  1:2495130/1‑93 (MQ=255)
                                      ggATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAata                                                   <  2:6665575/93‑1 (MQ=255)
                                       gATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataa                                                  <  1:2896382/93‑1 (MQ=255)
                                       gATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataa                                                  >  2:2922484/1‑93 (MQ=255)
                                        aTCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataac                                                 >  1:5058056/1‑93 (MQ=255)
                                        aTCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataac                                                 <  1:3153861/93‑1 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                <  2:5324140/93‑1 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                >  1:235035/1‑93 (MQ=255)
                                          ctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacat                                               >  1:3884740/1‑93 (MQ=255)
                                          ctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacat                                               <  2:1516211/93‑1 (MQ=255)
                                           tcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacata                                              <  2:3515185/93‑1 (MQ=255)
                                           tcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacata                                              >  1:4669343/1‑93 (MQ=255)
                                           tcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacata                                              >  1:4525834/1‑93 (MQ=255)
                                            cAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataa                                             >  2:1915172/1‑93 (MQ=255)
                                             aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataac                                            >  1:1622839/1‑93 (MQ=255)
                                             aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataac                                            >  2:2301481/1‑93 (MQ=255)
                                              aTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcc                                           >  1:81981/1‑93 (MQ=255)
                                                tttCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcccc                                         <  2:336197/93‑1 (MQ=255)
                                                 ttCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCa                                        <  1:4725020/93‑1 (MQ=255)
                                                  tCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCAt                                       <  1:5226296/93‑1 (MQ=255)
                                                  tCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAAAAACATAACCCCAt                                       >  1:6700878/1‑93 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     <  2:4698330/93‑1 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     <  2:601579/93‑1 (MQ=255)
                                                      ttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGata                                   <  1:6193973/93‑1 (MQ=255)
                                                        gCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatata                                 <  2:2683735/93‑1 (MQ=255)
                                                        gCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatata                                 <  2:879262/93‑1 (MQ=255)
                                                         cAATGAGACAGGAGCTTCCTGTTGTTATGGTATGGCACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatatat                                >  2:3982637/1‑93 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACGCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                               >  1:6254643/1‑93 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                               <  1:4873569/93‑1 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                               >  1:5274795/1‑93 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGCGATGCTGAATAACATAACCCCATGATATATc                               >  2:4998604/1‑93 (MQ=255)
                                                              agaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATa                           >  1:6061379/1‑93 (MQ=255)
                                                              agaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATa                           <  2:1125459/93‑1 (MQ=255)
                                                               gaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGGATAACATAACCCCATGATATATCGATaa                          >  1:2818856/1‑93 (MQ=255)
                                                               gaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaa                          >  1:5785593/1‑93 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        <  2:3025714/93‑1 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        >  2:798246/1‑93 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        <  2:69581/93‑1 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        <  1:1728599/93‑1 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        >  2:6172354/1‑93 (MQ=255)
                                                                   ggCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaata                      >  2:6414603/1‑93 (MQ=255)
                                                                   ggCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaata                      >  2:5297879/1‑93 (MQ=255)
                                                                     cgcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataat                    <  1:3856733/93‑1 (MQ=255)
                                                                        ttCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtctc                 >  2:1257116/1‑93 (MQ=255)
                                                                          ccTGTTGTGATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTa               <  2:6452772/93‑1 (MQ=255)
                                                                           cTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAc              >  2:4008138/1‑93 (MQ=255)
                                                                           cTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAc              >  1:1290756/1‑93 (MQ=255)
                                                                            tgttgtTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACa             >  2:4796731/1‑93 (MQ=255)
                                                                            tgttgtTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACa             <  1:398522/93‑1 (MQ=255)
                                                                             gttgttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAt            >  2:1342646/1‑93 (MQ=255)
                                                                             gttgttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAt            <  2:2271168/93‑1 (MQ=255)
                                                                             gttgttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGCGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAt            >  2:5141262/1‑93 (MQ=255)
                                                                                 ttATGGTATGGTACCCCGCTATTGAGCCGCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTGCATTTGa        <  1:6363275/93‑1 (MQ=255)
                                                                                  tatggtatggTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaa       >  2:1629376/1‑93 (MQ=255)
                                                                                     tgTATGGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAAt    <  2:5100921/92‑1 (MQ=255)
                                                                                     ggTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAAt    <  2:588828/93‑1 (MQ=255)
                                                                                      gTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATg   >  1:2148742/1‑93 (MQ=255)
                                                                                      gTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATg   <  2:3134401/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC  >  NC_000913/4259072‑4259251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  NC_000913/4259069‑4259255
                                                                                             |                                                                                             
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATG                                                                                               >  SRR4302536.490992/1‑94 (MQ=60)
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATG                                                                                               >  SRR4302536.3828211/1‑94 (MQ=60)
 GATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGG                                                                                              <  SRR4302536.4553071/94‑1 (MQ=60)
  ATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGT                                                                                             <  SRR4302536.3065120/94‑1 (MQ=60)
  ATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGT                                                                                             <  SRR4302536.6296496/94‑1 (MQ=60)
  ATTATTTACATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTGTGGTATGGT                                                                                             >  SRR4302536.5510863/1‑94 (MQ=60)
   TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTA                                                                                            <  SRR4302536.3002527/94‑1 (MQ=60)
   TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTA                                                                                            <  SRR4302536.6117608/94‑1 (MQ=60)
   TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTA                                                                                            >  SRR4302536.1253523/1‑94 (MQ=60)
         CCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGC                                                                                      >  SRR4302536.4711626/1‑94 (MQ=60)
         CCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGC                                                                                      >  SRR4302536.1475297/1‑94 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTTTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     <  SRR4302536.5340254/94‑1 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     >  SRR4302536.419271/1‑94 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     >  SRR4302536.4693111/1‑94 (MQ=60)
            TCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAT                                                                                   <  SRR4302536.4710268/94‑1 (MQ=60)
              ACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTG                                                                                 <  SRR4302536.3914888/94‑1 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGA                                                                                <  SRR4302536.2981082/94‑1 (MQ=60)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               <  SRR4302536.3587797/94‑1 (MQ=60)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               <  SRR4302536.335406/94‑1 (MQ=60)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               <  SRR4302536.759939/94‑1 (MQ=60)
                 TCGTGAGAGCTACATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGC                                                                              >  SRR4302536.2125592/1‑94 (MQ=60)
                  CGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCC                                                                             <  SRR4302536.6868845/94‑1 (MQ=60)
                   GTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCT                                                                            >  SRR4302536.3388501/1‑94 (MQ=60)
                   GTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCT                                                                            >  SRR4302536.5295969/1‑94 (MQ=60)
                   GTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCT                                                                            >  SRR4302536.453844/1‑94 (MQ=60)
                    TGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTC                                                                           >  SRR4302536.643778/1‑94 (MQ=60)
                     GAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCC                                                                          <  SRR4302536.6425283/94‑1 (MQ=60)
                     GAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCC                                                                          >  SRR4302536.922745/1‑94 (MQ=60)
                      AGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCT                                                                         <  SRR4302536.2114435/94‑1 (MQ=60)
                      AGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCT                                                                         >  SRR4302536.6542156/1‑94 (MQ=60)
                       GAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTG                                                                        >  SRR4302536.5846695/1‑94 (MQ=60)
                       GAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTG                                                                        <  SRR4302536.2042462/94‑1 (MQ=60)
                       AAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTG                                                                        <  SRR4302536.6617937/94‑1 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       <  SRR4302536.3177229/94‑1 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       >  SRR4302536.1181229/1‑94 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       >  SRR4302536.5874661/1‑94 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       >  SRR4302536.4183311/1‑94 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     >  SRR4302536.602677/1‑94 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     <  SRR4302536.4413606/94‑1 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     <  SRR4302536.4078715/94‑1 (MQ=60)
                           TTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATA                                                                    >  SRR4302536.5222318/1‑94 (MQ=60)
                           TTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATA                                                                    <  SRR4302536.3694900/94‑1 (MQ=60)
                           TTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATA                                                                    <  SRR4302536.4255871/94‑1 (MQ=60)
                               TGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGA                                                                >  SRR4302536.1214813/1‑94 (MQ=60)
                                GCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAT                                                               <  SRR4302536.139740/94‑1 (MQ=60)
                                GCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAT                                                               <  SRR4302536.5153962/94‑1 (MQ=60)
                                GCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAT                                                               <  SRR4302536.3925289/94‑1 (MQ=60)
                                 CCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATG                                                              <  SRR4302536.1463365/94‑1 (MQ=60)
                                 CCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATG                                                              <  SRR4302536.4667159/94‑1 (MQ=60)
                                 CCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATG                                                              <  SRR4302536.5718353/94‑1 (MQ=60)
                                  CTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGC                                                             >  SRR4302536.4821670/1‑94 (MQ=60)
                                   TTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCT                                                            <  SRR4302536.5863376/94‑1 (MQ=60)
                                   TTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCT                                                            <  SRR4302536.1965765/94‑1 (MQ=60)
                                     GAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGA                                                          >  SRR4302536.5562936/1‑94 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         >  SRR4302536.1699487/1‑94 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         >  SRR4302536.6722475/1‑94 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         >  SRR4302536.2748373/1‑94 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         <  SRR4302536.4909998/94‑1 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         <  SRR4302536.3993708/94‑1 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         >  SRR4302536.123480/1‑94 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       >  SRR4302536.4799112/1‑94 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       <  SRR4302536.5555030/94‑1 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       >  SRR4302536.5405017/1‑94 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       <  SRR4302536.4642104/94‑1 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       >  SRR4302536.4075433/1‑94 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       >  SRR4302536.2570387/1‑94 (MQ=60)
                                         GGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAA                                                      >  SRR4302536.1780725/1‑94 (MQ=60)
                                         GGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAA                                                      >  SRR4302536.394991/1‑94 (MQ=60)
                                          GATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAAC                                                     >  SRR4302536.983339/1‑94 (MQ=60)
                                          GATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAAC                                                     >  SRR4302536.5209568/1‑94 (MQ=60)
                                          GATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAAC                                                     <  SRR4302536.2981819/94‑1 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    >  SRR4302536.4473528/1‑94 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    >  SRR4302536.5239153/1‑94 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    >  SRR4302536.241280/1‑94 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    <  SRR4302536.4340315/94‑1 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    <  SRR4302536.3246223/94‑1 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTCAATAACA                                                    <  SRR4302536.5719925/94‑1 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   >  SRR4302536.4000293/1‑94 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   <  SRR4302536.1980515/94‑1 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   >  SRR4302536.689038/1‑94 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTAAATAACATA                                                  <  SRR4302536.6872413/94‑1 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  >  SRR4302536.4661544/1‑94 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  >  SRR4302536.4809736/1‑94 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  <  SRR4302536.496012/94‑1 (MQ=60)
                                              TCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAA                                                 <  SRR4302536.5470139/94‑1 (MQ=60)
                                               CAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAC                                                >  SRR4302536.3572657/1‑94 (MQ=60)
                                               CAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAC                                                >  SRR4302536.6581104/1‑94 (MQ=60)
                                               CAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAC                                                >  SRR4302536.1670101/1‑94 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               >  SRR4302536.84175/1‑94 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               <  SRR4302536.5573759/94‑1 (MQ=60)
                                                  TTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCC                                             <  SRR4302536.902055/94‑1 (MQ=60)
                                                  TTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCC                                             <  SRR4302536.5173052/94‑1 (MQ=60)
                                                   TTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCA                                            <  SRR4302536.5994411/94‑1 (MQ=60)
                                                   TTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCA                                            >  SRR4302536.2550782/1‑94 (MQ=60)
                                                    TTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCAT                                           <  SRR4302536.4867179/94‑1 (MQ=60)
                                                    TTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAAAAACATAACCCCAT                                           >  SRR4302536.6899906/1‑94 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302536.5382025/94‑1 (MQ=60)
                                                      CTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGA                                         >  SRR4302536.4836141/1‑94 (MQ=60)
                                                        TTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATA                                       >  SRR4302536.4306121/1‑94 (MQ=60)
                                                        TTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATA                                       >  SRR4302536.2990304/1‑94 (MQ=60)
                                                         TTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATAT                                      >  SRR4302536.2968509/1‑94 (MQ=60)
                                                         TTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATAT                                      <  SRR4302536.2717934/94‑1 (MQ=60)
                                                         TTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATAT                                      <  SRR4302536.6378470/94‑1 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACGCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302536.6440987/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302536.5432003/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   <  SRR4302536.130914/94‑1 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  >  SRR4302536.6687780/1‑94 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  <  SRR4302536.2862963/94‑1 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  <  SRR4302536.5020186/94‑1 (MQ=60)
                                                              ATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGA                                 >  SRR4302536.3369379/1‑94 (MQ=60)
                                                               TGAGACAGGCGCTTCCTGTTGTTATGGTATGTTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAT                                <  SRR4302536.239554/94‑1 (MQ=60)
                                                               TGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGTTATATCGAT                                <  SRR4302536.6757093/94‑1 (MQ=60)
                                                                GAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATA                               >  SRR4302536.6242208/1‑94 (MQ=60)
                                                                 AGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGGATAACATAACCCCATGATATATCGATAA                              >  SRR4302536.2902347/1‑94 (MQ=60)
                                                                 AGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAA                              >  SRR4302536.5957811/1‑94 (MQ=60)
                                                                 AGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAA                              <  SRR4302536.2802094/94‑1 (MQ=60)
                                                                 AGACAGGCGCTTCCTGTTGTTATGGGATGGTACTCCGCCATTGAGCCTCCTGAATAGTGATGCTGAATAAGATAACCCCATGATctatctgtaa                              >  SRR4302536.4981513/1‑84 (MQ=60)
                                                                   ACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAA                            >  SRR4302536.844338/1‑94 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           >  SRR4302536.4620583/1‑94 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           <  SRR4302536.1778511/94‑1 (MQ=60)
                                                                     AGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATA                          >  SRR4302536.582004/1‑94 (MQ=60)
                                                                      GGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAA                         >  SRR4302536.1590385/1‑94 (MQ=60)
                                                                        CGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATC                       <  SRR4302536.3971518/94‑1 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      <  SRR4302536.668480/94‑1 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATCGCACCCCGCTATTGAGCCTCCTGAATAGAGATGCTGAATAACATAACTCCATGATATATCGATAAAATAATCT                      >  SRR4302536.2595359/1‑94 (MQ=60)
                                                                          CTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTC                     <  SRR4302536.3830162/94‑1 (MQ=60)
                                                                          CTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTC                     >  SRR4302536.2009256/1‑94 (MQ=60)
                                                                           TTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCT                    <  SRR4302536.6223114/94‑1 (MQ=60)
                                                                             CCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAC                  >  SRR4302536.632140/1‑94 (MQ=60)
                                                                             CCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAC                  >  SRR4302536.1329352/1‑94 (MQ=60)
                                                                              CTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACA                 >  SRR4302536.3832658/1‑94 (MQ=60)
                                                                              CTGTTGTTAAGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACA                 >  SRR4302536.6742890/1‑94 (MQ=60)
                                                                               TGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCagcgg                <  SRR4302536.2314386/94‑6 (MQ=60)
                                                                               TGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAT                <  SRR4302536.4959611/94‑1 (MQ=60)
                                                                               TGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAT                <  SRR4302536.409066/94‑1 (MQ=60)
                                                                                  TGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTG             >  SRR4302536.5329535/1‑94 (MQ=60)
                                                                                  TGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTG             <  SRR4302536.657034/94‑1 (MQ=60)
                                                                                  TGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTG             <  SRR4302536.4876739/94‑1 (MQ=60)
                                                                                  TGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTG             <  SRR4302536.3196257/94‑1 (MQ=60)
                                                                                    TTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAA           <  SRR4302536.1886142/94‑1 (MQ=60)
                                                                                    TTATGGTATGGTACCCCGCTATTGAGCCGCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTGCATTTGAA           <  SRR4302536.6552864/94‑1 (MQ=60)
                                                                                     TATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAA          >  SRR4302536.1611173/1‑94 (MQ=60)
                                                                                       TGGTATGGTGCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAAT        <  SRR4302536.6254832/94‑1 (MQ=60)
                                                                                        GGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATG       >  SRR4302536.2213257/1‑94 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    <  SRR4302536.3469655/94‑1 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    <  SRR4302536.1533154/94‑1 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    <  SRR4302536.2784677/94‑1 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACG   <  SRR4302536.3641106/94‑1 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACG   >  SRR4302536.862489/1‑94 (MQ=60)
                                                                                             TGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  <  SRR4302536.5417611/94‑1 (MQ=60)
                                                                                             GGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  <  SRR4302536.5417610/94‑1 (MQ=60)
                                                                                             GGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  SRR4302536.2449102/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  NC_000913/4259069‑4259255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑