Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,597,072	T→C	D73G (GAC→GGC)	b3460 ←	LivJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,597,072	0	T	C	99.6%	1710.9 / ‑5.4	528	D73G (GAC→GGC)	b3460	LivJ
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (276/250); total (276/252)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

Only 100 of 543 total aligned reads displayed.

GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG  >  NC_000913/3596982‑3597161

gaagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCa                                                                                         <  1:6657352/93‑1 (MQ=255)

gaagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCa                                                                                         >  2:3487483/1‑93 (MQ=255)

gaagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCa                                                                                         <  1:3971003/93‑1 (MQ=255)

 aagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCTACCGCCTGTTTCGGGGCACAGGCATCGACat                                                                                        >  2:7616318/1‑93 (MQ=255)

 aagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCat                                                                                        <  2:9810020/93‑1 (MQ=255)

 aagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCat                                                                                        >  1:938127/1‑93 (MQ=255)

 aagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCat                                                                                        <  1:75414/93‑1 (MQ=255)

 aagaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCat                                                                                        >  2:4702973/1‑93 (MQ=255)

  agaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCata                                                                                       <  1:9138558/93‑1 (MQ=255)

  agaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCata                                                                                       >  1:6233033/1‑93 (MQ=255)

   gaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCatat                                                                                      >  1:2948809/1‑93 (MQ=255)

     aCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGTAACCGCCTGTTTCGGGTCACAGGCATCGCCATAttt                                                                                    >  2:1831826/1‑93 (MQ=255)

       agagGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTa                                                                                  >  2:10044444/1‑93 (MQ=255)

       agagGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTa                                                                                  <  2:3709658/93‑1 (MQ=255)

        gagGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTAc                                                                                 <  2:691314/93‑1 (MQ=255)

         agGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACg                                                                                <  1:2579746/93‑1 (MQ=255)

           gTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAt                                                                              >  1:8233319/1‑93 (MQ=255)

            tGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAtt                                                                             >  2:9570777/1‑93 (MQ=255)

            tGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAtt                                                                             <  1:1407162/93‑1 (MQ=255)

            tGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTGCGCCACCGCACCCTCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAtt                                                                             <  2:1327956/93‑1 (MQ=255)

             gACCAATCACATATTTAATGCCGTCGTTACCGACTTTGTTCGCCACCGCAACCACCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAttt                                                                            <  2:8564021/93‑1 (MQ=255)

             gACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAttt                                                                            >  1:9602777/1‑93 (MQ=255)

               ccAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGc                                                                          >  1:8035379/1‑93 (MQ=255)

                 aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAg                                                                        <  1:5184039/93‑1 (MQ=255)

                 aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAg                                                                        <  2:9004410/93‑1 (MQ=255)

                   tCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGtt                                                                      >  1:5916264/1‑93 (MQ=255)

                   tCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGtt                                                                      <  1:5123418/93‑1 (MQ=255)

                      caTATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgt                                                                   <  2:4445207/93‑1 (MQ=255)

                      caTATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgt                                                                   >  2:5907674/1‑93 (MQ=255)

                      caTATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGCCACAGGCATCGCCATATTTTACGATTTGCAGTttgt                                                                   >  1:5342184/1‑93 (MQ=255)

                        tatTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgttg                                                                 <  2:5613978/93‑1 (MQ=255)

                        tatTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgttg                                                                 >  1:270891/1‑93 (MQ=255)

                        tatTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgttg                                                                 >  1:7316262/1‑93 (MQ=255)

                        tatTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgttg                                                                 >  2:5050232/1‑93 (MQ=255)

                         atTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGc                                                                >  1:6100148/1‑93 (MQ=255)

                          tttAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGcc                                                               >  2:1408437/1‑93 (MQ=255)

                          tttAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGcc                                                               <  1:2431826/93‑1 (MQ=255)

                           ttAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCt                                                              >  2:5778272/1‑93 (MQ=255)

                           ttAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCt                                                              >  2:278910/1‑93 (MQ=255)

                             aaTGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCttt                                                            <  2:6329406/93‑1 (MQ=255)

                               tGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTaa                                                          >  2:6053904/1‑93 (MQ=255)

                               tGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTaa                                                          <  2:2361838/93‑1 (MQ=255)

                                tccGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAt                                                         <  2:8430421/92‑1 (MQ=255)

                                 ccGTCGTTAACGACTTTGTTTGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATg                                                        <  2:3205873/93‑1 (MQ=255)

                                   gtcgtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgcc                                                      >  1:7928214/1‑93 (MQ=255)

                                    tcgtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgccg                                                     >  1:3584510/1‑93 (MQ=255)

                                       ttAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCt                                                  >  1:1075475/1‑93 (MQ=255)

                                       ttAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCt                                                  <  2:8307634/93‑1 (MQ=255)

                                       ttAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCt                                                  >  1:5653417/1‑93 (MQ=255)

                                        tAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCtt                                                 >  1:888995/1‑93 (MQ=255)

                                        tAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCtt                                                 >  1:4607784/1‑93 (MQ=255)

                                         aaCGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCttt                                                <  1:4376785/93‑1 (MQ=255)

                                          aCGCCTTGGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTa                                               <  2:2548105/93‑1 (MQ=255)

                                          aCGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTa                                               <  1:8756031/93‑1 (MQ=255)

                                          aCGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTa                                               >  1:6526251/1‑93 (MQ=255)

                                           cGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAg                                              >  2:1922651/1‑93 (MQ=255)

                                            gACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGc                                             <  2:4081551/93‑1 (MQ=255)

                                             aCTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCg                                            >  1:7808276/1‑93 (MQ=255)

                                             aCTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCg                                            >  2:3042375/1‑93 (MQ=255)

                                               tttGTTCGCGACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGtt                                          <  1:1771373/93‑1 (MQ=255)

                                               tttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGtt                                          <  2:6679005/93‑1 (MQ=255)

                                               tttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGtt                                          >  1:8111794/1‑93 (MQ=255)

                                               tttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGtt                                          >  1:2198318/1‑93 (MQ=255)

                                                ttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTg                                         >  2:3417154/1‑93 (MQ=255)

                                                 tGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGa                                        >  1:4723416/1‑93 (MQ=255)

                                                   ttCGCCACCGCAACCGACTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGata                                      <  2:7505016/93‑1 (MQ=255)

                                                     cGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATc                                    <  2:8241548/93‑1 (MQ=255)

                                                      gCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATcc                                   <  1:5494027/93‑1 (MQ=255)

                                                      cccACCGCAACGTCCTGTTTCGGGTCACAGGCATCTCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATcc                                   <  2:4282224/92‑1 (MQ=255)

                                                       ccACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCg                                  >  1:9864142/1‑93 (MQ=255)

                                                       ccACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCg                                  <  1:3230048/93‑1 (MQ=255)

                                                        cACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGc                                 <  1:1177122/93‑1 (MQ=255)

                                                         aCCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCa                                >  2:4143433/1‑93 (MQ=255)

                                                         aCCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCa                                >  1:8406861/1‑93 (MQ=255)

                                                           cGCACCCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAAc                              <  2:6557205/93‑1 (MQ=255)

                                                              aaCCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTAAATGCCGCCTTTAGCGTTGATATCCGCAACCGc                           >  1:372861/1‑93 (MQ=255)

                                                               aCCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGcc                          <  1:7713742/93‑1 (MQ=255)

                                                                ccGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCt                         >  2:9233836/1‑93 (MQ=255)

                                                                  gCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGc                       <  2:7330219/93‑1 (MQ=255)

                                                                  gCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGc                       >  1:5821169/1‑93 (MQ=255)

                                                                   ccTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGct                      >  1:4238587/1‑93 (MQ=255)

                                                                      gTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTg                   <  2:8927440/93‑1 (MQ=255)

                                                                         tCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTgcgc                >  1:8330189/1‑93 (MQ=255)

                                                                         tCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTgcgc                >  1:7171537/1‑93 (MQ=255)

                                                                             gTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGt            >  2:1658045/1‑93 (MQ=255)

                                                                             gTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGt            >  2:9392243/1‑93 (MQ=255)

                                                                             gTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGt            >  2:29096/1‑93 (MQ=255)

                                                                             gTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGt            >  2:5397652/1‑93 (MQ=255)

                                                                              gcacaGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTa           <  2:9703910/92‑1 (MQ=255)

                                                                               tacaGGCATCCGCATATTTTACGATTTGCAGTTTGTTCCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTaa          <  1:3355151/92‑1 (MQ=255)

                                                                               cacaGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTaa          >  1:9741102/1‑93 (MQ=255)

                                                                               cacaGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTaa          <  2:8738466/93‑1 (MQ=255)

                                                                                acaGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTaaa         <  2:2064987/93‑1 (MQ=255)

                                                                                 caGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAAc        <  1:10009761/93‑1 (MQ=255)

                                                                                  aGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACt       >  1:6893476/1‑93 (MQ=255)

                                                                                   ggCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTc      <  2:7876858/93‑1 (MQ=255)

                                                                                   ggCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTc      >  2:1185979/1‑93 (MQ=255)

                                                                                      aTCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTg   <  1:5448708/93‑1 (MQ=255)

                                                                                       tCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTgg  >  1:2025656/1‑93 (MQ=255)

                                                                                       tCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTgg  <  1:4077617/93‑1 (MQ=255)

GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG  >  NC_000913/3596982‑3597161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 295 total aligned reads displayed.

GATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  NC_000913/3596979‑3597165

GATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGC                                                                                               >  SRR4302540.7649942/1‑94 (MQ=60)

 ATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCC                                                                                              >  SRR4302540.4828134/1‑94 (MQ=60)

  TGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCA                                                                                             >  SRR4302540.4374287/1‑94 (MQ=60)

   GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCAT                                                                                            <  SRR4302540.4013811/94‑1 (MQ=60)

   GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCAT                                                                                            >  SRR4302540.678850/1‑94 (MQ=60)

    AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATA                                                                                           <  SRR4302540.9829480/94‑1 (MQ=60)

    AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATA                                                                                           >  SRR4302540.6302353/1‑94 (MQ=60)

     AGAACAGAGGTGACCAATCACNTNTTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAT                                                                                          <  SRR4302540.2347513/94‑1 (MQ=60)

     AGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAT                                                                                          <  SRR4302540.4047503/94‑1 (MQ=60)

     AGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAT                                                                                          <  SRR4302540.9248357/94‑1 (MQ=60)

      GAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATT                                                                                         <  SRR4302540.8875286/94‑1 (MQ=60)

      AAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATT                                                                                         <  SRR4302540.7854889/94‑1 (MQ=60)

      AAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATT                                                                                         <  SRR4302540.7515923/94‑1 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        <  SRR4302540.10133312/94‑1 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        >  SRR4302540.7323335/1‑94 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATAGCCATATTT                                                                                        >  SRR4302540.4323662/1‑94 (MQ=60)

        ACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTT                                                                                       <  SRR4302540.1423675/94‑1 (MQ=60)

        ACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTT                                                                                       >  SRR4302540.667917/1‑94 (MQ=60)

          AGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTAC                                                                                     >  SRR4302540.9283702/1‑94 (MQ=60)

           GAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACG                                                                                    <  SRR4302540.3055873/94‑1 (MQ=60)

            AGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGA                                                                                   >  SRR4302540.49978/1‑94 (MQ=60)

            AGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGA                                                                                   <  SRR4302540.2607560/94‑1 (MQ=60)

             GGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAT                                                                                  >  SRR4302540.2207083/1‑94 (MQ=60)

             GGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAT                                                                                  >  SRR4302540.8329022/1‑94 (MQ=60)

              GTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATT                                                                                 >  SRR4302540.2981805/1‑94 (MQ=60)

              GTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATT                                                                                 >  SRR4302540.5220405/1‑94 (MQ=60)

              GTGACCAATCACATATTTAATGCCGTCGTTAAAGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATT                                                                                 >  SRR4302540.9940928/1‑94 (MQ=60)

               TGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTT                                                                                <  SRR4302540.2141587/94‑1 (MQ=60)

               TGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTT                                                                                >  SRR4302540.7150691/1‑94 (MQ=60)

               TGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTT                                                                                <  SRR4302540.3567318/94‑1 (MQ=60)

               TGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTT                                                                                <  SRR4302540.1422315/94‑1 (MQ=60)

               TGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTT                                                                                >  SRR4302540.9716050/1‑94 (MQ=60)

               TGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCCCAACCCCCGTTTTCGGGCCACAGGCATCCCCATATTTTACGATTT                                                                                >  SRR4302540.5246366/1‑94 (MQ=60)

                 ACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGC                                                                              >  SRR4302540.8128774/1‑94 (MQ=60)

                  CCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCA                                                                             >  SRR4302540.8458748/1‑94 (MQ=60)

                  CCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCA                                                                             >  SRR4302540.4955900/1‑94 (MQ=60)

                   CAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCTCCGCCGTCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAG                                                                            <  SRR4302540.8437086/94‑1 (MQ=60)

                   CAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAG                                                                            >  SRR4302540.8791731/1‑94 (MQ=60)

                   CAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAG                                                                            >  SRR4302540.3300193/1‑94 (MQ=60)

                    AATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGT                                                                           <  SRR4302540.5240473/94‑1 (MQ=60)

                    AATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGT                                                                           >  SRR4302540.5203257/1‑94 (MQ=60)

                     ATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCGCAGGTGTCGCCATCTTTTACGATTTGCAGCT                                                                          >  SRR4302540.3694044/1‑94 (MQ=60)

                     ATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTT                                                                          >  SRR4302540.7912356/1‑94 (MQ=60)

                     ATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTT                                                                          >  SRR4302540.6085708/1‑94 (MQ=60)

                     ATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTT                                                                          >  SRR4302540.5981620/1‑94 (MQ=60)

                     ATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGGT                                                                          >  SRR4302540.9559211/1‑94 (MQ=60)

                      TCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         <  SRR4302540.6252451/94‑1 (MQ=60)

                      TCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         <  SRR4302540.4030542/94‑1 (MQ=60)

                      TCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         <  SRR4302540.5178899/94‑1 (MQ=60)

                       CACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTG                                                                        <  SRR4302540.1277855/94‑1 (MQ=60)

                       CACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTG                                                                        >  SRR4302540.8555373/1‑94 (MQ=60)

                         CATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTT                                                                      <  SRR4302540.4714182/94‑1 (MQ=60)

                         CATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTT                                                                      <  SRR4302540.5448403/94‑1 (MQ=60)

                         CATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTT                                                                      <  SRR4302540.8013056/94‑1 (MQ=60)

                         CATATCTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCACCCTCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTT                                                                      <  SRR4302540.5646095/94‑1 (MQ=60)

                         CAGATTTAATGCCGTTGTTAACGCCTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTT                                                                      <  SRR4302540.4505222/94‑1 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     >  SRR4302540.7397966/1‑94 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     <  SRR4302540.9487614/94‑1 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     <  SRR4302540.9304005/94‑1 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     <  SRR4302540.83985/94‑1 (MQ=60)

                           TATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGC                                                                    >  SRR4302540.6168060/1‑94 (MQ=60)

                           TATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGC                                                                    >  SRR4302540.1856715/1‑94 (MQ=60)

                            ATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCT                                                                   >  SRR4302540.8233270/1‑94 (MQ=60)

                            ATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCC                                                                   >  SRR4302540.10138170/1‑94 (MQ=60)

                            ATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCC                                                                   >  SRR4302540.10000789/1‑94 (MQ=60)

                            ATTGAATCCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCC                                                                   <  SRR4302540.2937857/94‑1 (MQ=60)

                             TTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCT                                                                  <  SRR4302540.2458026/94‑1 (MQ=60)

                              TTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTT                                                                 >  SRR4302540.2672159/1‑94 (MQ=60)

                               TAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTT                                                                >  SRR4302540.7894027/1‑94 (MQ=60)

                               TAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTT                                                                <  SRR4302540.8121627/94‑1 (MQ=60)

                               TAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTT                                                                >  SRR4302540.5409129/1‑94 (MQ=60)

                                AATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTA                                                               >  SRR4302540.5655135/1‑94 (MQ=60)

                                  TGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAT                                                             <  SRR4302540.3265229/94‑1 (MQ=60)

                                  TGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAT                                                             >  SRR4302540.10283/1‑94 (MQ=60)

                                  TCCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAT                                                             <  SRR4302540.1141952/94‑1 (MQ=60)

                                   GCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATG                                                            <  SRR4302540.8311525/94‑1 (MQ=60)

                                    CCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGC                                                           >  SRR4302540.6384235/1‑94 (MQ=60)

                                    CCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGC                                                           >  SRR4302540.9753208/1‑94 (MQ=60)

                                    CCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGC                                                           <  SRR4302540.3553113/94‑1 (MQ=60)

                                    CCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGC                                                           >  SRR4302540.5526523/1‑94 (MQ=60)

                                     CGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCC                                                          >  SRR4302540.8020615/1‑94 (MQ=60)

                                      GTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTtcgtatttcagtttgtttccttttactccg                                                         >  SRR4302540.905840/1‑64 (MQ=60)

                                      GTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCG                                                         >  SRR4302540.3623665/1‑94 (MQ=60)

                                      GTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCG                                                         >  SRR4302540.9854498/1‑94 (MQ=60)

                                      GTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCG                                                         >  SRR4302540.5776657/1‑94 (MQ=60)

                                        CGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCC                                                       >  SRR4302540.9955269/1‑94 (MQ=60)

                                        CGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCC                                                       >  SRR4302540.683592/1‑94 (MQ=60)

                                         GTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCT                                                      >  SRR4302540.5715660/1‑94 (MQ=60)

                                         GTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCT                                                      >  SRR4302540.1086466/1‑94 (MQ=60)

                                         GTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTAACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCT                                                      >  SRR4302540.3899808/1‑94 (MQ=60)

                                          TTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTT                                                     <  SRR4302540.3037829/94‑1 (MQ=60)

                                          TTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTT                                                     <  SRR4302540.5679538/94‑1 (MQ=60)

                                          TTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTT                                                     >  SRR4302540.898440/1‑94 (MQ=60)

                                          TTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTT                                                     >  SRR4302540.4657266/1‑94 (MQ=60)

                                           TAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTT                                                    <  SRR4302540.9564784/94‑1 (MQ=60)

                                           TAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTT                                                    >  SRR4302540.1320279/1‑94 (MQ=60)

                                            AACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTA                                                   >  SRR4302540.7603671/1‑94 (MQ=60)

                                            AACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTA                                                   >  SRR4302540.6598729/1‑94 (MQ=60)

                                            AACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTA                                                   >  SRR4302540.3468522/1‑94 (MQ=60)

                                            AACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTA                                                   <  SRR4302540.4424310/94‑1 (MQ=60)

                                            AACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTA                                                   <  SRR4302540.2819956/94‑1 (MQ=60)

                                            AACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGATTGTTGCCTTTAACGCCGCCTTTA                                                   >  SRR4302540.1636068/1‑94 (MQ=60)

                                             ACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAG                                                  >  SRR4302540.8899240/1‑94 (MQ=60)

                                             ACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAG                                                  <  SRR4302540.8101605/94‑1 (MQ=60)

                                              CGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGC                                                 <  SRR4302540.5516335/94‑1 (MQ=60)

                                              CGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGC                                                 <  SRR4302540.7538469/94‑1 (MQ=60)

                                              CGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGC                                                 <  SRR4302540.5266280/94‑1 (MQ=60)

                                              CGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGC                                                 >  SRR4302540.2044474/1‑94 (MQ=60)

                                               GACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCG                                                >  SRR4302540.9927311/1‑94 (MQ=60)

                                               GACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCG                                                >  SRR4302540.1775242/1‑94 (MQ=60)

                                                ACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGT                                               >  SRR4302540.9509551/1‑94 (MQ=60)

                                                ACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGT                                               <  SRR4302540.6942105/94‑1 (MQ=60)

                                                ACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTGTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGT                                               <  SRR4302540.6942106/94‑1 (MQ=60)

                                                 CTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTT                                              <  SRR4302540.3972688/94‑1 (MQ=60)

                                                 CTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTT                                              <  SRR4302540.2468604/94‑1 (MQ=60)

                                                 CTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTT                                              >  SRR4302540.8205943/1‑94 (MQ=60)

                                                 CTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTT                                              >  SRR4302540.2222055/1‑94 (MQ=60)

                                                 CTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTT                                              <  SRR4302540.8959239/94‑1 (MQ=60)

                                                  TTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTG                                             <  SRR4302540.2809986/94‑1 (MQ=60)

                                                  TTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTG                                             <  SRR4302540.8737870/94‑1 (MQ=60)

                                                  TTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTG                                             <  SRR4302540.7050321/94‑1 (MQ=60)

                                                  TTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTG                                             <  SRR4302540.7050319/94‑1 (MQ=60)

                                                   TTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGA                                            >  SRR4302540.4773867/1‑94 (MQ=60)

                                                     GTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATA                                          <  SRR4302540.4509029/94‑1 (MQ=60)

                                                      TTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATAT                                         <  SRR4302540.8165978/94‑1 (MQ=60)

                                                      TTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATAT                                         <  SRR4302540.8478972/94‑1 (MQ=60)

                                                       TCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATC                                        <  SRR4302540.5461468/94‑1 (MQ=60)

                                                        CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCC                                       <  SRR4302540.7784661/94‑1 (MQ=60)

                                                        CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCC                                       <  SRR4302540.5376940/94‑1 (MQ=60)

                                                         GCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCG                                      >  SRR4302540.6667271/1‑94 (MQ=60)

                                                         GCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCG                                      <  SRR4302540.5554513/94‑1 (MQ=60)

                                                          CCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGC                                     <  SRR4302540.3265175/94‑1 (MQ=60)

                                                          CCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGC                                     >  SRR4302540.8244426/1‑94 (MQ=60)

                                                          CCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGC                                     >  SRR4302540.1931447/1‑94 (MQ=60)

                                                           CACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCA                                    >  SRR4302540.8393550/1‑94 (MQ=60)

                                                           CACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCA                                    >  SRR4302540.7994413/1‑94 (MQ=60)

                                                           CACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCA                                    <  SRR4302540.1189287/94‑1 (MQ=60)

                                                            CCCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   <  SRR4302540.4115109/94‑1 (MQ=60)

                                                            CCCGCAACCCCCTGTGTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   <  SRR4302540.4141683/94‑1 (MQ=60)

                                                            ACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   <  SRR4302540.4141684/94‑1 (MQ=60)

                                                            ACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   >  SRR4302540.5543609/1‑94 (MQ=60)

                                                              CGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACC                                 <  SRR4302540.1957378/94‑1 (MQ=60)

                                                               GCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCG                                >  SRR4302540.2665487/1‑94 (MQ=60)

                                                                CAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTAAATGCCGCCTTTAGCGTTGATATCCGCAACCGC                               >  SRR4302540.376714/1‑94 (MQ=60)

                                                                 AACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCC                              <  SRR4302540.2275610/94‑1 (MQ=60)

                                                                 AACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCC                              >  SRR4302540.4960824/1‑94 (MQ=60)

                                                                 AACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCC                              >  SRR4302540.4258490/1‑94 (MQ=60)

                                                                 AACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCC                              >  SRR4302540.3698555/1‑94 (MQ=60)

                                                                  ACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCT                             <  SRR4302540.7809227/94‑1 (MQ=60)

                                                                  ACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATGTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCT                             <  SRR4302540.7802631/94‑1 (MQ=60)

                                                                    CGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGC                           >  SRR4302540.5885512/1‑94 (MQ=60)

                                                                    CGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGC                           >  SRR4302540.6423449/1‑94 (MQ=60)

                                                                    CGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGC                           >  SRR4302540.5417520/1‑94 (MQ=60)

                                                                    CGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGC                           >  SRR4302540.5885511/1‑94 (MQ=60)

                                                                    CGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGC                           <  SRR4302540.6270039/94‑1 (MQ=60)

                                                                    CGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGC                           <  SRR4302540.37242/94‑1 (MQ=60)

                                                                     GCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCT                          >  SRR4302540.8185344/1‑94 (MQ=60)

                                                                     GCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCT                          >  SRR4302540.4284706/1‑94 (MQ=60)

                                                                     GCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCT                          >  SRR4302540.1200129/1‑94 (MQ=60)

                                                                     GCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCT                          >  SRR4302540.5939207/1‑94 (MQ=60)

                                                                      CCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTC                         >  SRR4302540.3294406/1‑94 (MQ=60)

                                                                       CTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCT                        >  SRR4302540.9088922/1‑94 (MQ=60)

                                                                       CTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCT                        <  SRR4302540.7632144/94‑1 (MQ=60)

                                                                           TTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGC                    >  SRR4302540.7251477/1‑94 (MQ=60)

                                                                           TTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGC                    >  SRR4302540.4647678/1‑94 (MQ=60)

                                                                           TTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGC                    >  SRR4302540.8426876/1‑94 (MQ=60)

                                                                            TCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCC                   >  SRR4302540.3651169/1‑94 (MQ=60)

                                                                             CGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCG                  >  SRR4302540.2836168/1‑94 (MQ=60)

                                                                              GGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGG                 >  SRR4302540.6643733/1‑94 (MQ=60)

                                                                              GGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGG                 >  SRR4302540.6166180/1‑94 (MQ=60)

                                                                              GGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGG                 >  SRR4302540.7346539/1‑94 (MQ=60)

                                                                               GGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGT                >  SRR4302540.9215814/1‑94 (MQ=60)

                                                                               GGTCACAGGCATCACCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGATCTGCGCCGGT                <  SRR4302540.9071723/94‑1 (MQ=60)

                                                                                GTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTA               <  SRR4302540.255717/94‑1 (MQ=60)

                                                                                 TCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAA              <  SRR4302540.6427044/94‑1 (MQ=60)

                                                                                  TACAGGCATCCGCATATTTTACGATTTGCAGTTTGTTCCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAA             <  SRR4302540.3391921/94‑1 (MQ=60)

                                                                                  CACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAC             >  SRR4302540.6064672/1‑94 (MQ=60)

                                                                                   ACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAAC            <  SRR4302540.5663221/94‑1 (MQ=60)

                                                                                   ACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAAC            >  SRR4302540.590839/1‑94 (MQ=60)

                                                                                   ACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAAC            <  SRR4302540.7774769/94‑1 (MQ=60)

                                                                                     AGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTC          >  SRR4302540.809727/1‑94 (MQ=60)

                                                                                      GGCATCGCCATATTTTACGATTTGCAGTTTGTTGCGTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCC         <  SRR4302540.2664608/94‑1 (MQ=60)

                                                                                      GGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATACCCGCAACCGCCTGCTCTGCGCCGGTAAACTCC         <  SRR4302540.8629114/94‑1 (MQ=60)

                                                                                       GCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCT        >  SRR4302540.1141603/1‑94 (MQ=60)

                                                                                        CATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTG       <  SRR4302540.7303253/94‑1 (MQ=60)

                                                                                        CATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTG       >  SRR4302540.5846065/1‑94 (MQ=60)

                                                                                        CATCGCCATATTTTACGATTTGCAGTTTGTTCCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTG       <  SRR4302540.2361346/94‑1 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      >  SRR4302540.4695351/1‑94 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      <  SRR4302540.10048655/94‑1 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      >  SRR4302540.1006410/1‑94 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      >  SRR4302540.2047538/1‑94 (MQ=60)

                                                                                          TCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGT     >  SRR4302540.4316748/1‑94 (MQ=60)

                                                                                          TCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGT     <  SRR4302540.4121580/94‑1 (MQ=60)

                                                                                          TCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGT     >  SRR4302540.992977/1‑94 (MQ=60)

                                                                                           CGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTC    >  SRR4302540.2563041/1‑94 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCC   >  SRR4302540.8265637/1‑94 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCA   >  SRR4302540.6192855/1‑94 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCA   >  SRR4302540.8265636/1‑94 (MQ=60)

                                                                                             CCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  <  SRR4302540.6033326/94‑1 (MQ=60)

                                                                                             CCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  SRR4302540.5186997/1‑94 (MQ=60)

GATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  NC_000913/3596979‑3597165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`