Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,744,796 G→A G28D (GGC→GAC)  lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,744,7960GA99.2% 1228.2 / ‑4.0 358G28D (GGC→GAC) lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base A (165/189);  total (169/189)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 398 total aligned reads displayed.

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA  >  NC_000913/3744706‑3744888
                                                                                          |                                                                                            
aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACg                                                                                            >  2:1710373/1‑93 (MQ=255)
 aTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGt                                                                                           <  1:4891374/93‑1 (MQ=255)
  tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTg                                                                                          >  2:9758462/1‑93 (MQ=255)
  tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTg                                                                                          >  2:6102623/1‑93 (MQ=255)
    aaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCa                                                                                        >  2:5423872/1‑93 (MQ=255)
     aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAg                                                                                       <  2:8281296/93‑1 (MQ=255)
      ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgc                                                                                      >  1:5318203/1‑93 (MQ=255)
         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                   <  1:1018823/93‑1 (MQ=255)
         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                   >  2:4758860/1‑93 (MQ=255)
         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                   >  2:9405323/1‑93 (MQ=255)
           aCGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTg                                                                                 <  1:3470838/93‑1 (MQ=255)
             gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGcc                                                                               <  2:8581696/93‑1 (MQ=255)
             gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGcc                                                                               >  1:5145900/1‑93 (MQ=255)
               aaTTCTGGCTGGGGTTAGATTGTGGCGTTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCGGGGAGGCAGACGTGCAGCTCCTGCCGc                                                                             <  2:6026562/93‑1 (MQ=255)
               aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             <  1:8502760/93‑1 (MQ=255)
               aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             >  1:3635575/1‑93 (MQ=255)
               aaTACTGGATGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             <  2:891982/93‑1 (MQ=255)
                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                           <  1:7326884/93‑1 (MQ=255)
                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                           <  2:2864363/93‑1 (MQ=255)
                    tggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgc                                                                        >  1:4419062/1‑93 (MQ=255)
                      gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGGCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcgc                                                                      <  1:9018263/93‑1 (MQ=255)
                           ggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                                 <  1:6025039/93‑1 (MQ=255)
                           ggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                                 <  1:2792336/93‑1 (MQ=255)
                            gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                <  2:7962399/93‑1 (MQ=255)
                             ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                               <  1:2625148/93‑1 (MQ=255)
                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              <  1:1847021/93‑1 (MQ=255)
                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              <  2:2283790/93‑1 (MQ=255)
                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              <  1:7489020/93‑1 (MQ=255)
                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                             >  2:5882384/1‑93 (MQ=255)
                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                           >  2:1382034/1‑93 (MQ=255)
                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                           <  2:5268970/93‑1 (MQ=255)
                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                           <  2:3038703/93‑1 (MQ=255)
                                  ttGTGGCGGTAGCTGCCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                          <  1:165624/93‑1 (MQ=255)
                                    gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                        <  2:9877244/93‑1 (MQ=255)
                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                      <  1:6575304/93‑1 (MQ=255)
                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                      <  2:4704805/93‑1 (MQ=255)
                                       gCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAg                                                     <  2:5702156/93‑1 (MQ=255)
                                        cGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAgg                                                    >  2:179705/1‑93 (MQ=255)
                                          gTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCt                                                  >  1:1478335/1‑93 (MQ=255)
                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                 <  1:2129908/93‑1 (MQ=255)
                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                 <  1:5588256/93‑1 (MQ=255)
                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                 >  1:2337635/1‑93 (MQ=255)
                                            aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                                >  1:4134663/1‑93 (MQ=255)
                                              ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                              >  1:1044754/1‑93 (MQ=255)
                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCa                                             <  1:9522744/93‑1 (MQ=255)
                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                            >  2:1363697/1‑93 (MQ=255)
                                                 gttgAGAGCCGGGCTGGATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           <  2:1412213/91‑1 (MQ=255)
                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           <  2:10002704/93‑1 (MQ=255)
                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                          <  1:9280655/93‑1 (MQ=255)
                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                          >  2:8146952/1‑93 (MQ=255)
                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                          <  2:1561395/93‑1 (MQ=255)
                                                   tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagagg                                         >  2:1182147/1‑92 (MQ=255)
                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                        <  1:6370242/93‑1 (MQ=255)
                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                        >  2:7184682/1‑93 (MQ=255)
                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                      >  2:4356063/1‑93 (MQ=255)
                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                      >  2:6618406/1‑93 (MQ=255)
                                                       acgcGGACTGTATGACCGCGAAGGCGGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGCCTGGGCAGAGCGCGa                                     <  2:125200/90‑1 (MQ=255)
                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  1:3874358/93‑1 (MQ=255)
                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  2:1987873/93‑1 (MQ=255)
                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  1:1377125/93‑1 (MQ=255)
                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  2:577853/93‑1 (MQ=255)
                                                          ggggTTGTATGCCCGAGAGGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                  <  2:9634468/92‑1 (MQ=255)
                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                  <  2:485733/93‑1 (MQ=255)
                                                           gggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                                 <  2:3371507/93‑1 (MQ=255)
                                                           gggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                                 <  2:3709418/93‑1 (MQ=255)
                                                             gCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatattga                               >  1:453525/1‑90 (MQ=255)
                                                             gCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGc                               <  2:7797608/93‑1 (MQ=255)
                                                             gCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGc                               <  1:5428716/93‑1 (MQ=255)
                                                                gTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGa                            <  2:263986/93‑1 (MQ=255)
                                                                gTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGa                            >  1:5784110/1‑93 (MQ=255)
                                                                 tATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGAGATGTAGCAGaa                           >  2:915841/1‑93 (MQ=255)
                                                                  aTGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAc                          >  1:6970193/1‑93 (MQ=255)
                                                                   tGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACt                         <  1:619842/93‑1 (MQ=255)
                                                                     aCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                       >  1:3029494/1‑93 (MQ=255)
                                                                     aCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                       <  2:3542707/93‑1 (MQ=255)
                                                                        gcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGAAGAACTGTGGc                    <  2:6549455/93‑1 (MQ=255)
                                                                        gcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAAGGCAGAACTGTGGc                    >  1:8695922/1‑93 (MQ=255)
                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGACAGCGCGATATGGCAGAACTGTGGCaa                  >  1:2281841/1‑93 (MQ=255)
                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa                  <  1:5002207/93‑1 (MQ=255)
                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa                  <  1:126597/93‑1 (MQ=255)
                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                 >  1:3819643/1‑93 (MQ=255)
                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                 >  1:8557737/1‑93 (MQ=255)
                                                                            aGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtg                >  2:6824015/1‑93 (MQ=255)
                                                                              gCCGGGCGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGCGGTATGGCAGAAATGTGGCAAtgct              >  2:4003988/1‑93 (MQ=255)
                                                                              gCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct              <  2:873271/93‑1 (MQ=255)
                                                                                 ggggggCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa           <  1:7183847/93‑1 (MQ=255)
                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa           <  1:9823503/93‑1 (MQ=255)
                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa           >  1:4588827/1‑93 (MQ=255)
                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGGTATGGCAGGACTGTGGCAATGCTGCAt          >  1:2405161/1‑93 (MQ=255)
                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          >  2:2599399/1‑93 (MQ=255)
                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          >  1:8159156/1‑93 (MQ=255)
                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          >  1:7866974/1‑93 (MQ=255)
                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          <  2:5068756/93‑1 (MQ=255)
                                                                                   gAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATg         <  2:247907/93‑1 (MQ=255)
                                                                                   gAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATg         <  2:6412796/93‑1 (MQ=255)
                                                                                    aggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg        <  1:6755213/93‑1 (MQ=255)
                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       <  2:8424912/93‑1 (MQ=255)
                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       >  2:7652088/1‑93 (MQ=255)
                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       >  2:7544255/1‑93 (MQ=255)
                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       >  2:6330306/1‑93 (MQ=255)
                                                                                          |                                                                                            
AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA  >  NC_000913/3744706‑3744888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 205 total aligned reads displayed.
TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  >  NC_000913/3744703‑3744889
                                                                                             |                                                                                             
TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGA                                                                                               <  SRR4302540.4427811/94‑1 (MQ=60)
 GCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTCGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGAC                                                                                              <  SRR4302540.866642/94‑1 (MQ=60)
  CAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACG                                                                                             <  SRR4302540.4918532/94‑1 (MQ=60)
   AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGG                                                                                            >  SRR4302540.4610352/1‑94 (MQ=60)
    ATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTG                                                                                           <  SRR4302540.4943970/94‑1 (MQ=60)
    ATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTG                                                                                           <  SRR4302540.6097646/94‑1 (MQ=60)
      AAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCA                                                                                         <  SRR4302540.672534/94‑1 (MQ=60)
       AACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAG                                                                                        >  SRR4302540.631378/1‑94 (MQ=60)
        ACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGC                                                                                       <  SRR4302540.1738329/94‑1 (MQ=60)
        ACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGC                                                                                       >  SRR4302540.5376436/1‑94 (MQ=60)
        ACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGA                                                                                       >  SRR4302540.9923043/1‑94 (MQ=60)
         CCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCG                                                                                      >  SRR4302540.3826650/1‑94 (MQ=60)
          CATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGC                                                                                     >  SRR4302540.1389927/1‑94 (MQ=60)
            TGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCT                                                                                   <  SRR4302540.7540206/94‑1 (MQ=60)
            TGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCT                                                                                   <  SRR4302540.1650475/94‑1 (MQ=60)
            TGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCT                                                                                   <  SRR4302540.1029162/94‑1 (MQ=60)
             GACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTG                                                                                  <  SRR4302540.4000271/94‑1 (MQ=60)
              ACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGC                                                                                 <  SRR4302540.3508624/94‑1 (MQ=60)
              ACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGC                                                                                 >  SRR4302540.5463821/1‑94 (MQ=60)
              ACGCAATACTGGCTGGGGTTAGATTGCGGCGGTAGCGGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGC                                                                                 >  SRR4302540.5463822/1‑94 (MQ=60)
               CGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGCGGCAGACGTGCAGCGCCTGCC                                                                                <  SRR4302540.4842406/94‑1 (MQ=60)
               CGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCC                                                                                >  SRR4302540.6893543/1‑94 (MQ=60)
               CGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCC                                                                                >  SRR4302540.5201642/1‑94 (MQ=60)
               CGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCC                                                                                >  SRR4302540.1501626/1‑94 (MQ=60)
                GCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCG                                                                               >  SRR4302540.14308/1‑94 (MQ=60)
                 CAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGC                                                                              >  SRR4302540.3675372/1‑94 (MQ=60)
                 CAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGC                                                                              >  SRR4302540.1043992/1‑94 (MQ=60)
                  AATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCT                                                                             <  SRR4302540.8601567/94‑1 (MQ=60)
                  AATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTCCCGCT                                                                             >  SRR4302540.5029501/1‑94 (MQ=60)
                    TACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGT                                                                           >  SRR4302540.7474810/1‑94 (MQ=60)
                    TACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGT                                                                           <  SRR4302540.7408693/94‑1 (MQ=60)
                      CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGC                                                                         >  SRR4302540.4724780/1‑94 (MQ=60)
                      CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGC                                                                         >  SRR4302540.4466940/1‑94 (MQ=60)
                      CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGC                                                                         >  SRR4302540.1253523/1‑94 (MQ=60)
                      CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGC                                                                         >  SRR4302540.2810702/1‑94 (MQ=60)
                      CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGAATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTACCGCTGTGC                                                                         >  SRR4302540.3227959/1‑94 (MQ=60)
                       TGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCG                                                                        <  SRR4302540.5657898/94‑1 (MQ=60)
                        GGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGC                                                                       >  SRR4302540.5238821/1‑94 (MQ=60)
                         GCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGGCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCA                                                                      <  SRR4302540.9126473/94‑1 (MQ=60)
                            GGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTA                                                                   <  SRR4302540.4709678/94‑1 (MQ=60)
                             GGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAA                                                                  >  SRR4302540.1326629/1‑94 (MQ=60)
                             GGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAA                                                                  <  SRR4302540.3909077/94‑1 (MQ=60)
                             GGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGAAGTGCAGCGCCTGCCGCTGTGGGCATTAA                                                                  >  SRR4302540.1326628/1‑94 (MQ=60)
                              GGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAG                                                                 <  SRR4302540.2822483/94‑1 (MQ=60)
                              GGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAG                                                                 <  SRR4302540.5046310/94‑1 (MQ=60)
                              GGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAG                                                                 <  SRR4302540.6091896/94‑1 (MQ=60)
                              GGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAG                                                                 <  SRR4302540.1779668/94‑1 (MQ=60)
                              GGTTAGATTGTGGCGGTAGCTGGCCGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAG                                                                 >  SRR4302540.4279957/1‑94 (MQ=60)
                               GTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGC                                                                >  SRR4302540.2647793/1‑94 (MQ=60)
                               GTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGGATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGC                                                                >  SRR4302540.10058243/1‑94 (MQ=60)
                               GTTAGATTGTGCGGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGC                                                                <  SRR4302540.5089693/94‑1 (MQ=60)
                                TTNGATTNTGGCGGTAGCTGGNTGAAAGCCGGGCTGTATGACCGCAAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCC                                                               <  SRR4302540.5970925/94‑1 (MQ=60)
                                TTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCC                                                               <  SRR4302540.2653536/94‑1 (MQ=60)
                                 TAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCC                                                              <  SRR4302540.1867270/94‑1 (MQ=60)
                                 TAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCC                                                              <  SRR4302540.3646124/94‑1 (MQ=60)
                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGTTGTATGACCGCGAAGGCCGGGAGGCGGACGGGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           <  SRR4302540.9911843/94‑1 (MQ=60)
                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           >  SRR4302540.7769651/1‑94 (MQ=60)
                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           <  SRR4302540.2377108/94‑1 (MQ=60)
                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           >  SRR4302540.7769650/1‑94 (MQ=60)
                                     TTGTGGCGGTAGCTGCCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAG                                                          <  SRR4302540.167281/94‑1 (MQ=60)
                                      TGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGC                                                         <  SRR4302540.2440747/94‑1 (MQ=60)
                                      TGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGACCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGC                                                         >  SRR4302540.979744/1‑94 (MQ=60)
                                       GTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGCCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCC                                                        >  SRR4302540.3662995/1‑94 (MQ=60)
                                       GTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCC                                                        <  SRR4302540.6190359/94‑1 (MQ=60)
                                       GTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCC                                                        <  SRR4302540.7302253/94‑1 (MQ=60)
                                       GTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTCCCGCTGTGCGCATTAAGCCCGCAGCC                                                        <  SRR4302540.591359/94‑1 (MQ=60)
                                         GGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAG                                                      <  SRR4302540.6648140/94‑1 (MQ=60)
                                         GGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAG                                                      <  SRR4302540.6523820/94‑1 (MQ=60)
                                         GGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAG                                                      >  SRR4302540.5555637/1‑94 (MQ=60)
                                           CGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGC                                                    >  SRR4302540.1737479/1‑94 (MQ=60)
                                           CGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGC                                                    <  SRR4302540.4954921/94‑1 (MQ=60)
                                            GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGTCT                                                   >  SRR4302540.8339579/1‑94 (MQ=60)
                                            GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCT                                                   >  SRR4302540.8633778/1‑94 (MQ=60)
                                            GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCT                                                   <  SRR4302540.10114456/94‑1 (MQ=60)
                                            GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCT                                                   >  SRR4302540.1494521/1‑94 (MQ=60)
                                             GTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTG                                                  >  SRR4302540.2362826/1‑94 (MQ=60)
                                             GTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTG                                                  >  SRR4302540.1398387/1‑94 (MQ=60)
                                             GTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTG                                                  >  SRR4302540.1398388/1‑94 (MQ=60)
                                             GTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTG                                                  >  SRR4302540.5181598/1‑94 (MQ=60)
                                             GTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTG                                                  >  SRR4302540.5775538/1‑94 (MQ=60)
                                              TAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGG                                                 <  SRR4302540.9511010/94‑1 (MQ=60)
                                              TAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGG                                                 >  SRR4302540.4179393/1‑94 (MQ=60)
                                              TAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGG                                                 <  SRR4302540.5321138/94‑1 (MQ=60)
                                              TAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGG                                                 <  SRR4302540.2152761/94‑1 (MQ=60)
                                              TAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGG                                                 <  SRR4302540.7308337/94‑1 (MQ=60)
                                               AGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                >  SRR4302540.446928/1‑94 (MQ=60)
                                               AGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                <  SRR4302540.6562029/94‑1 (MQ=60)
                                                GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               <  SRR4302540.862599/94‑1 (MQ=60)
                                                GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               >  SRR4302540.807138/1‑94 (MQ=60)
                                                GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               >  SRR4302540.1055370/1‑94 (MQ=60)
                                                GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               >  SRR4302540.1055369/1‑94 (MQ=60)
                                                 CTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCA                                              <  SRR4302540.2304972/94‑1 (MQ=60)
                                                 CTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCA                                              <  SRR4302540.6573221/94‑1 (MQ=60)
                                                 CTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCA                                              >  SRR4302540.3890434/1‑94 (MQ=60)
                                                  TGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGgac                                             >  SRR4302540.5506475/1‑91 (MQ=60)
                                                  TGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAG                                             <  SRR4302540.9635505/94‑1 (MQ=60)
                                                  TGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAG                                             <  SRR4302540.9497211/94‑1 (MQ=60)
                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302540.9391585/94‑1 (MQ=60)
                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302540.223001/94‑1 (MQ=60)
                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302540.9391584/94‑1 (MQ=60)
                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302540.2854700/94‑1 (MQ=60)
                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          >  SRR4302540.7912589/1‑94 (MQ=60)
                                                       GAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGC                                        <  SRR4302540.6441177/94‑1 (MQ=60)
                                                       GAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGC                                        <  SRR4302540.2098921/94‑1 (MQ=60)
                                                       GAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGC                                        >  SRR4302540.2899386/1‑94 (MQ=60)
                                                        AAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCG                                       >  SRR4302540.9320994/1‑94 (MQ=60)
                                                        AAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCG                                       >  SRR4302540.4485235/1‑94 (MQ=60)
                                                        AAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCG                                       >  SRR4302540.2314305/1‑94 (MQ=60)
                                                         AAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGA                                      <  SRR4302540.2647238/94‑1 (MQ=60)
                                                          AGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGAT                                     <  SRR4302540.4472771/94‑1 (MQ=60)
                                                           GCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATA                                    <  SRR4302540.6919912/94‑1 (MQ=60)
                                                            CCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAT                                   <  SRR4302540.1391771/94‑1 (MQ=60)
                                                            CCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAT                                   <  SRR4302540.3916468/94‑1 (MQ=60)
                                                            CCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAT                                   <  SRR4302540.7965334/94‑1 (MQ=60)
                                                             CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  >  SRR4302540.9275491/1‑94 (MQ=60)
                                                             CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  >  SRR4302540.2710429/1‑94 (MQ=60)
                                                             CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  >  SRR4302540.8729861/1‑94 (MQ=60)
                                                              GGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGG                                 >  SRR4302540.2815530/1‑94 (MQ=60)
                                                              GGGATGTATGACCGCGAAGGCCGGTATGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGG                                 <  SRR4302540.643443/94‑1 (MQ=60)
                                                               GGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATtga                                >  SRR4302540.458365/1‑91 (MQ=60)
                                                               GGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGC                                <  SRR4302540.6363555/94‑1 (MQ=60)
                                                                GCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               <  SRR4302540.4629690/94‑1 (MQ=60)
                                                                GCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               <  SRR4302540.5488423/94‑1 (MQ=60)
                                                                GCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               <  SRR4302540.3410285/94‑1 (MQ=60)
                                                                 CTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATTGCAG                              >  SRR4302540.693993/1‑94 (MQ=60)
                                                                 CTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAG                              >  SRR4302540.8993159/1‑94 (MQ=60)
                                                                 CTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAG                              >  SRR4302540.8771095/1‑94 (MQ=60)
                                                                 CTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAG                              >  SRR4302540.8337265/1‑94 (MQ=60)
                                                                  TGTATGACCGCGAAGGCGGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGA                             <  SRR4302540.5308251/94‑1 (MQ=60)
                                                                  TGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGA                             >  SRR4302540.5847998/1‑94 (MQ=60)
                                                                  TGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGA                             >  SRR4302540.715319/1‑94 (MQ=60)
                                                                  TGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGA                             >  SRR4302540.8626493/1‑94 (MQ=60)
                                                                   GTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAA                            >  SRR4302540.5141831/1‑94 (MQ=60)
                                                                    ttcgcCCGCGAAGGCCGGGAGGCAGACGGGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAC                           <  SRR4302540.2792392/89‑1 (MQ=60)
                                                                    TGTGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGCGATATGGCAGAAC                           >  SRR4302540.8339514/1‑94 (MQ=60)
                                                                    TATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAC                           >  SRR4302540.6207420/1‑94 (MQ=60)
                                                                    TATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAC                           >  SRR4302540.7047674/1‑94 (MQ=60)
                                                                    TATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAC                           >  SRR4302540.5422459/1‑94 (MQ=60)
                                                                    TATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAC                           <  SRR4302540.2309565/94‑1 (MQ=60)
                                                                     ATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACT                          <  SRR4302540.4390733/94‑1 (MQ=60)
                                                                     ATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACT                          <  SRR4302540.3873067/94‑1 (MQ=60)
                                                                      TGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTG                         <  SRR4302540.5657731/94‑1 (MQ=60)
                                                                      TGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTG                         <  SRR4302540.626440/94‑1 (MQ=60)
                                                                       GACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGT                        >  SRR4302540.3062765/1‑94 (MQ=60)
                                                                        ACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTG                       <  SRR4302540.5991795/94‑1 (MQ=60)
                                                                         CCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGG                      <  SRR4302540.5523556/94‑1 (MQ=60)
                                                                         CCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGG                      <  SRR4302540.2189185/94‑1 (MQ=60)
                                                                          CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGC                     >  SRR4302540.9494699/1‑94 (MQ=60)
                                                                          CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGC                     <  SRR4302540.8771982/94‑1 (MQ=60)
                                                                          CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAAGGCAGAACTGTGGC                     >  SRR4302540.8796873/1‑94 (MQ=60)
                                                                            CGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGACAGCGCGATATGGCAGAACTGTGGCAA                   >  SRR4302540.2306359/1‑94 (MQ=60)
                                                                            CGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAA                   <  SRR4302540.4392517/94‑1 (MQ=60)
                                                                            CGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAA                   <  SRR4302540.399995/94‑1 (MQ=60)
                                                                             GACGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCgtcgcgcgatatgggagccctgtggcaag                  >  SRR4302540.6703737/1‑65 (MQ=60)
                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCCAT                  >  SRR4302540.7180517/1‑94 (MQ=60)
                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  >  SRR4302540.6703738/1‑94 (MQ=60)
                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  <  SRR4302540.127937/94‑1 (MQ=60)
                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  >  SRR4302540.3861222/1‑94 (MQ=60)
                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  >  SRR4302540.8657137/1‑94 (MQ=60)
                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  <  SRR4302540.5056376/94‑1 (MQ=60)
                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  <  SRR4302540.1955138/94‑1 (MQ=60)
                                                                                  CCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGC             <  SRR4302540.2394774/94‑1 (MQ=60)
                                                                                   CGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCA            >  SRR4302540.1535527/1‑94 (MQ=60)
                                                                                   CGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCA            >  SRR4302540.4638234/1‑94 (MQ=60)
                                                                                    GGGGGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.7263932/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGGTATGGCAGGACTGTGGCAATGCTGCAT           >  SRR4302540.2431100/1‑94 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.9938427/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           >  SRR4302540.7958882/1‑94 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.9819304/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.3841759/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.1111904/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.1880187/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.3072847/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           >  SRR4302540.8253891/1‑94 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.4586423/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.3509071/94‑1 (MQ=60)
                                                                                    GGGAGGCAGACGCGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302540.3072848/94‑1 (MQ=60)
                                                                                     GGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATG          <  SRR4302540.1242076/94‑1 (MQ=60)
                                                                                     GGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATG          <  SRR4302540.7556387/94‑1 (MQ=60)
                                                                                      GAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGTCTGGGCAGAGCGCGCTATGACCGAACTGTCGCCATTCTGCATGG         >  SRR4302540.3799811/1‑94 (MQ=60)
                                                                                       AGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGC        <  SRR4302540.6829854/94‑1 (MQ=60)
                                                                                       AGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGC        >  SRR4302540.9114927/1‑94 (MQ=60)
                                                                                        GGCATACGTGCAGCGCCTGCCGCTGGTCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCT       <  SRR4302540.3662941/94‑1 (MQ=60)
                                                                                        GGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCT       <  SRR4302540.3981497/94‑1 (MQ=60)
                                                                                        GGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCT       <  SRR4302540.7130370/94‑1 (MQ=60)
                                                                                        GGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCT       <  SRR4302540.2894242/94‑1 (MQ=60)
                                                                                         GCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTG      <  SRR4302540.8174405/94‑1 (MQ=60)
                                                                                         GCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTG      >  SRR4302540.23642/1‑94 (MQ=60)
                                                                                           AGACGTGCAGCGCCTGCCGCTGTGCGCATTACGGCCGCAGCCAGGCTGGGCAGAGCCCGCCATGGCAGAACTGTGGGAATGCTGCATGGCTGCC    >  SRR4302540.9194034/1‑94 (MQ=60)
                                                                                           AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGGTGCATGGCTGTC    >  SRR4302540.1261869/1‑94 (MQ=60)
                                                                                           AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTC    >  SRR4302540.9705763/1‑94 (MQ=60)
                                                                                           AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTC    >  SRR4302540.9194035/1‑94 (MQ=60)
                                                                                            GGGGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA   <  SRR4302540.6756715/94‑1 (MQ=60)
                                                                                            GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGGAATGCTGCATGGCTGTCT   >  SRR4302540.7009733/1‑94 (MQ=60)
                                                                                            GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGGAATGCTGCATGGCTGTCA   >  SRR4302540.5446017/1‑94 (MQ=60)
                                                                                            GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCCTGGCTGTCA   >  SRR4302540.5276310/1‑94 (MQ=60)
                                                                                            GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA   >  SRR4302540.1050434/1‑94 (MQ=60)
                                                                                            GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGATGGGCAGAGCGCGAGATGGCAGAACTGTCGCAATGCTCCATGGCTGTac   >  SRR4302540.5022105/1‑92 (MQ=60)
                                                                                             ACGTGCAGCGCCTGCCGCTGTGGGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  <  SRR4302540.1852858/94‑1 (MQ=60)
                                                                                             ACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  <  SRR4302540.1852859/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  >  NC_000913/3744703‑3744889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑