Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,793,037 A→G Q161R (CAG→CGG)  rfaF → ADP‑heptose‑‑lps heptosyltransferase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,793,0370AG99.8% 1631.3 / ‑6.2 504Q161R (CAG→CGG) rfaFADP‑heptose‑‑lps heptosyltransferase II
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (261/241);  total (262/242)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 529 total aligned reads displayed.

TGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGT  >  NC_000913/3792947‑3793126
                                                                                          |                                                                                         
tggtggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCggg                                                                                         >  1:813513/1‑93 (MQ=255)
tggtggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCggg                                                                                         >  1:48017/1‑93 (MQ=255)
tggtggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCggg                                                                                         >  2:4132252/1‑93 (MQ=255)
   tggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGa                                                                                      <  1:7769902/93‑1 (MQ=255)
    ggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAg                                                                                     <  2:8217694/93‑1 (MQ=255)
      aaCGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCg                                                                                   >  2:7382575/1‑93 (MQ=255)
        agCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGaa                                                                                 <  1:10041376/92‑1 (MQ=255)
          cTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAgg                                                                               >  2:9481163/1‑93 (MQ=255)
           tatataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAggg                                                                              >  1:6088424/1‑92 (MQ=255)
           tatataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGt                                                                              >  2:7283964/1‑93 (MQ=255)
           tatataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGt                                                                              <  1:6233598/93‑1 (MQ=255)
             tataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGa                                                                            <  2:2683374/93‑1 (MQ=255)
             tataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGa                                                                            <  2:7948367/93‑1 (MQ=255)
              ataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaa                                                                           >  1:8789532/1‑93 (MQ=255)
              ataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaa                                                                           <  1:5254318/93‑1 (MQ=255)
                aGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaa                                                                         >  2:3177120/1‑93 (MQ=255)
                 gcgcTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaaa                                                                        <  1:7387300/93‑1 (MQ=255)
                 gcgcTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaaa                                                                        <  1:8530326/93‑1 (MQ=255)
                  cgcTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAAt                                                                       >  2:6263498/1‑93 (MQ=255)
                      ggCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCata                                                                   <  1:6905867/93‑1 (MQ=255)
                      ggCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCata                                                                   <  1:5671631/93‑1 (MQ=255)
                       gCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCatat                                                                  >  1:9338526/1‑93 (MQ=255)
                       gCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCatat                                                                  <  2:6805527/93‑1 (MQ=255)
                         cTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAc                                                                <  2:7048098/93‑1 (MQ=255)
                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               >  2:2358491/1‑93 (MQ=255)
                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               >  1:4937743/1‑93 (MQ=255)
                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               >  1:4005555/1‑93 (MQ=255)
                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               >  2:3347091/1‑93 (MQ=255)
                            tGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTg                                                             >  1:9919661/1‑93 (MQ=255)
                            tGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTg                                                             <  1:3350124/93‑1 (MQ=255)
                             gACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGt                                                            >  2:904030/1‑93 (MQ=255)
                              aCAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTa                                                           >  2:1011135/1‑93 (MQ=255)
                               cAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTaa                                                          >  2:3666354/1‑93 (MQ=255)
                                 aaGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATc                                                        >  2:1386347/1‑93 (MQ=255)
                                  aGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCa                                                       >  2:9614500/1‑93 (MQ=255)
                                   gtcATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGGAGGTGCAATATCATATACCTGTCATcgc                                                      >  2:5199176/3‑91 (MQ=255)
                                     cATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAtt                                                    >  1:6699975/1‑93 (MQ=255)
                                     cATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAtt                                                    >  1:5444030/1‑93 (MQ=255)
                                      aTTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAttt                                                   >  2:1226988/1‑93 (MQ=255)
                                      aTTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAttt                                                   <  1:9203433/93‑1 (MQ=255)
                                       ttATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAtttt                                                  >  2:7520783/1‑93 (MQ=255)
                                       ttATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAtttt                                                  >  1:5028706/1‑93 (MQ=255)
                                       ttATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAtttt                                                  >  2:4591012/1‑93 (MQ=255)
                                        tATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTc                                                 <  2:648080/93‑1 (MQ=255)
                                        tATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTc                                                 <  1:3909981/93‑1 (MQ=255)
                                         aTGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCg                                                <  2:5996828/93‑1 (MQ=255)
                                         aTGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCg                                                >  2:388248/1‑93 (MQ=255)
                                          tGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGc                                               <  1:434243/93‑1 (MQ=255)
                                          tGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGc                                               >  2:1859294/1‑93 (MQ=255)
                                            cgcACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCtt                                             <  2:5554614/93‑1 (MQ=255)
                                            cgcACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCtt                                             <  1:2926010/93‑1 (MQ=255)
                                            cgcACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCtt                                             <  2:8945724/93‑1 (MQ=255)
                                             gcacagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCttt                                            <  2:2195044/93‑1 (MQ=255)
                                              cacagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtc                                           <  1:1321165/93‑1 (MQ=255)
                                              cacagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtc                                           >  2:6097365/1‑93 (MQ=255)
                                               acagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtca                                          <  2:829960/93‑1 (MQ=255)
                                                cagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtcat                                         <  2:1515292/93‑1 (MQ=255)
                                                 agcacaAGATCTGCCGCAGCCAGTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtcatc                                        <  2:8476784/93‑1 (MQ=255)
                                                  gcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtcatca                                       <  1:2641427/93‑1 (MQ=255)
                                                  gcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtcatca                                       >  2:5715682/1‑93 (MQ=255)
                                                   cacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAACATCATATACCTGTAATCAATTTTCGCTTTCATCAg                                      <  1:1825331/93‑1 (MQ=255)
                                                    acaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGa                                     >  1:6555919/1‑93 (MQ=255)
                                                     caAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGaa                                    >  2:7192801/1‑93 (MQ=255)
                                                     caAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGaa                                    <  1:8745177/93‑1 (MQ=255)
                                                      aaGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAc                                   >  1:2003816/1‑93 (MQ=255)
                                                      aaGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAc                                   <  2:8827154/93‑1 (MQ=255)
                                                      aaGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAc                                   <  2:9281158/93‑1 (MQ=255)
                                                          tCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTcc                               <  2:7796957/93‑1 (MQ=255)
                                                             gCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCgat                            <  2:3032070/93‑1 (MQ=255)
                                                               cGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCgatga                          >  1:818718/1‑93 (MQ=255)
                                                                gCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCgatgat                         <  1:9555882/93‑1 (MQ=255)
                                                                gCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCgatgat                         <  1:8992930/93‑1 (MQ=255)
                                                                gCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCgatgat                         >  2:5454990/1‑93 (MQ=255)
                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        >  2:2030009/1‑93 (MQ=255)
                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        <  2:9172272/93‑1 (MQ=255)
                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        >  1:3178612/1‑93 (MQ=255)
                                                                 cAGCCATTGCTATGGCCGCAGTTACGGATGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        <  1:4526466/93‑1 (MQ=255)
                                                                   gCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTgg                      >  2:4863988/1‑93 (MQ=255)
                                                                   gCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTgg                      >  1:9831234/1‑93 (MQ=255)
                                                                    ccATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGt                     >  1:3030353/1‑93 (MQ=255)
                                                                    ccATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGt                     >  1:3805341/1‑93 (MQ=255)
                                                                      aTTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGttt                   <  1:3498865/93‑1 (MQ=255)
                                                                        tGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGttttt                 >  2:2907203/1‑93 (MQ=255)
                                                                          ttATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGc               <  1:5101123/93‑1 (MQ=255)
                                                                           tATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGcc              >  2:3459513/1‑93 (MQ=255)
                                                                            aTGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGccc             <  1:247747/93‑1 (MQ=255)
                                                                             tGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCg            >  1:7989331/1‑93 (MQ=255)
                                                                             tGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATAAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCg            >  2:871784/1‑93 (MQ=255)
                                                                              ggCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCgg           <  1:1503479/93‑1 (MQ=255)
                                                                              ggCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCgg           <  2:6632578/93‑1 (MQ=255)
                                                                                ccGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGt         >  2:3755341/1‑93 (MQ=255)
                                                                                  gCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTTGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGgcgc       >  2:8652298/1‑93 (MQ=255)
                                                                                  gCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGc       >  1:7122842/1‑93 (MQ=255)
                                                                                  gCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGc       >  1:2133184/1‑93 (MQ=255)
                                                                                    aGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCgg     >  1:1188610/1‑93 (MQ=255)
                                                                                     gTTGCGGGTGAGCGAAGGTGAAAAATCATATGCCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGa    <  2:4986762/93‑1 (MQ=255)
                                                                                     gTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGa    >  1:4157452/1‑93 (MQ=255)
                                                                                     gTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGa    <  2:2467247/93‑1 (MQ=255)
                                                                                     gTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGa    >  1:5525069/1‑93 (MQ=255)
                                                                                      ttGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAg   >  2:4414963/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGT  >  NC_000913/3792947‑3793126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 272 total aligned reads displayed.
TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGG  >  NC_000913/3792944‑3793130
                                                                                             |                                                                                             
TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCG                                                                                               <  SRR4302540.238997/94‑1 (MQ=60)
TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCG                                                                                               >  SRR4302540.7556659/1‑94 (MQ=60)
 AATGGTGGAACGCCATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGG                                                                                              <  SRR4302540.922938/94‑1 (MQ=60)
  ATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACATCACAAGATCTGCCCCAGCCATTGTTATGGCCGCAGTTGCGGG                                                                                             >  SRR4302540.3045933/1‑94 (MQ=60)
  ATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGG                                                                                             >  SRR4302540.822153/1‑94 (MQ=60)
  ATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGG                                                                                             >  SRR4302540.48503/1‑94 (MQ=60)
  ATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGG                                                                                             <  SRR4302540.496738/94‑1 (MQ=60)
  ATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGG                                                                                             >  SRR4302540.38820/1‑94 (MQ=60)
  ATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGG                                                                                             >  SRR4302540.1144476/1‑94 (MQ=60)
  ATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGG                                                                                             >  SRR4302540.1916532/1‑94 (MQ=60)
   TGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGT                                                                                            >  SRR4302540.5852485/1‑94 (MQ=60)
     GTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGA                                                                                          <  SRR4302540.3644536/94‑1 (MQ=60)
      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         >  SRR4302540.8913920/1‑94 (MQ=60)
      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         <  SRR4302540.3571913/94‑1 (MQ=60)
      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         >  SRR4302540.8241632/1‑94 (MQ=60)
      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         <  SRR4302540.1318725/94‑1 (MQ=60)
       GGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGC                                                                                        <  SRR4302540.519511/94‑1 (MQ=60)
          ACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAA                                                                                     <  SRR4302540.8888065/94‑1 (MQ=60)
          ACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAA                                                                                     >  SRR4302540.4515814/1‑94 (MQ=60)
          ACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGCTGCGGGTGAGCGAA                                                                                     >  SRR4302540.9909877/1‑94 (MQ=60)
           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    <  SRR4302540.9821784/94‑1 (MQ=60)
           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    >  SRR4302540.3213594/1‑94 (MQ=60)
           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    >  SRR4302540.2504678/1‑94 (MQ=60)
           AGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    <  SRR4302540.10158127/94‑1 (MQ=60)
             CTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGT                                                                                  >  SRR4302540.1985050/1‑94 (MQ=60)
             CTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGT                                                                                  <  SRR4302540.6335245/94‑1 (MQ=60)
             CTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGG                                                                                  >  SRR4302540.6156183/1‑94 (MQ=60)
             CTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGG                                                                                  >  SRR4302540.5635888/1‑94 (MQ=60)
             CTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGG                                                                                  >  SRR4302540.732572/1‑94 (MQ=60)
              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 <  SRR4302540.6302925/94‑1 (MQ=60)
              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 <  SRR4302540.6302924/94‑1 (MQ=60)
              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 >  SRR4302540.9200469/1‑94 (MQ=60)
              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 <  SRR4302540.9305424/94‑1 (MQ=60)
              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 >  SRR4302540.4129858/1‑94 (MQ=60)
              TAGATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 <  SRR4302540.4549057/94‑1 (MQ=60)
                TATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAA                                                                               >  SRR4302540.8891229/1‑94 (MQ=60)
                TATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAA                                                                               >  SRR4302540.4920739/1‑94 (MQ=60)
                 ATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAA                                                                              <  SRR4302540.5311628/94‑1 (MQ=60)
                   AGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAA                                                                            <  SRR4302540.5106789/94‑1 (MQ=60)
                   AGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAA                                                                            >  SRR4302540.3521596/1‑94 (MQ=60)
                    GCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCTTTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAAT                                                                           <  SRR4302540.8629439/94‑1 (MQ=60)
                    GCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAAT                                                                           <  SRR4302540.5028476/94‑1 (MQ=60)
                     CGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATC                                                                          <  SRR4302540.8442339/94‑1 (MQ=60)
                     CGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATC                                                                          <  SRR4302540.5395916/94‑1 (MQ=60)
                      GCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCA                                                                         >  SRR4302540.741844/1‑94 (MQ=60)
                       CTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCAT                                                                        >  SRR4302540.8233703/1‑94 (MQ=60)
                       CTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATAAT                                                                        >  SRR4302540.5779070/1‑94 (MQ=60)
                        TGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATA                                                                       >  SRR4302540.4677442/1‑94 (MQ=60)
                         GGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATAT                                                                      <  SRR4302540.6982656/94‑1 (MQ=60)
                         GGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATAT                                                                      <  SRR4302540.3839259/94‑1 (MQ=60)
                         GGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATAT                                                                      >  SRR4302540.508001/1‑94 (MQ=60)
                         GGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATAT                                                                      <  SRR4302540.5734079/94‑1 (MQ=60)
                         GGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATAT                                                                      >  SRR4302540.9449824/1‑94 (MQ=60)
                          GCCTATGACAAAGGCATTATGCGCACAGCACAATATCTGCCGCAGCCATTGTTATGGCCGTAGTTGCGGTTTAGTGAAGGTGAAAAATCATATA                                                                     >  SRR4302540.9029164/1‑94 (MQ=60)
                          GCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATA                                                                     >  SRR4302540.3960898/1‑94 (MQ=60)
                           CCTATGACAAAGGCATTATGAGCACAGCACATGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGTAAAATCATATAC                                                                    >  SRR4302540.690638/1‑94 (MQ=60)
                            CTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGGAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACT                                                                   >  SRR4302540.4904493/1‑94 (MQ=60)
                            CTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACC                                                                   >  SRR4302540.4048683/1‑94 (MQ=60)
                            CTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACC                                                                   >  SRR4302540.4991017/1‑94 (MQ=60)
                            CTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACC                                                                   <  SRR4302540.9985584/94‑1 (MQ=60)
                            CTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACC                                                                   <  SRR4302540.6921872/94‑1 (MQ=60)
                             TATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCT                                                                  <  SRR4302540.5337581/94‑1 (MQ=60)
                              ATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTG                                                                 <  SRR4302540.3940920/94‑1 (MQ=60)
                              ATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTG                                                                 >  SRR4302540.10035503/1‑94 (MQ=60)
                              ATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTG                                                                 >  SRR4302540.8890731/1‑94 (MQ=60)
                              ATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTG                                                                 >  SRR4302540.183750/1‑94 (MQ=60)
                               TGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGT                                                                <  SRR4302540.3386853/94‑1 (MQ=60)
                               TGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGT                                                                <  SRR4302540.3386852/94‑1 (MQ=60)
                               TGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGT                                                                <  SRR4302540.7580700/94‑1 (MQ=60)
                                GACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTA                                                               >  SRR4302540.1587267/1‑94 (MQ=60)
                                 ACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAA                                                              >  SRR4302540.6137279/1‑94 (MQ=60)
                                 ACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAA                                                              <  SRR4302540.4261135/94‑1 (MQ=60)
                                 ACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAA                                                              <  SRR4302540.421064/94‑1 (MQ=60)
                                  AAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAAT                                                             <  SRR4302540.9415513/94‑1 (MQ=60)
                                   AAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATC                                                            <  SRR4302540.7085499/94‑1 (MQ=60)
                                    AAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCA                                                           >  SRR4302540.5602419/1‑94 (MQ=60)
                                     AGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAA                                                          >  SRR4302540.3493396/1‑94 (MQ=60)
                                     AGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAA                                                          <  SRR4302540.5103387/94‑1 (MQ=60)
                                     AGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAA                                                          >  SRR4302540.317770/1‑94 (MQ=60)
                                      GGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAT                                                         <  SRR4302540.6800682/94‑1 (MQ=60)
                                       GCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATT                                                        >  SRR4302540.2636434/1‑94 (MQ=60)
                                       GCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATT                                                        >  SRR4302540.6773932/1‑94 (MQ=60)
                                       GCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATT                                                        >  SRR4302540.5503935/1‑94 (MQ=60)
                                        CATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTT                                                       >  SRR4302540.2980538/1‑94 (MQ=60)
                                        CATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTT                                                       >  SRR4302540.6656748/1‑94 (MQ=60)
                                        CATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTT                                                       <  SRR4302540.1122597/94‑1 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTTAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      >  SRR4302540.8648555/1‑94 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      >  SRR4302540.5083127/1‑94 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      <  SRR4302540.5549250/94‑1 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      >  SRR4302540.10022472/1‑94 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      >  SRR4302540.4314523/1‑94 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      <  SRR4302540.9313862/94‑1 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      >  SRR4302540.9469672/1‑94 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      >  SRR4302540.9469670/1‑94 (MQ=60)
                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      <  SRR4302540.25727/94‑1 (MQ=60)
                                         ACTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      <  SRR4302540.7048547/94‑1 (MQ=60)
                                           TATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCG                                                    <  SRR4302540.3952354/94‑1 (MQ=60)
                                             TGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCT                                                  <  SRR4302540.438837/94‑1 (MQ=60)
                                             TGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCT                                                  >  SRR4302540.6050316/1‑94 (MQ=60)
                                              GCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTT                                                 <  SRR4302540.2234609/94‑1 (MQ=60)
                                              GCGCACAGCACAAGATCTGCCGCAGCCATTGCTATGGCCGCAGATGCGGGGGAGCGAAGGTGAAAGATCATATACCTGTAATCACTTTTCGCTT                                                 >  SRR4302540.3365559/1‑94 (MQ=60)
                                               CGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTT                                                >  SRR4302540.2130132/1‑94 (MQ=60)
                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               >  SRR4302540.468093/1‑94 (MQ=60)
                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               >  SRR4302540.3321625/1‑94 (MQ=60)
                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               <  SRR4302540.311968/94‑1 (MQ=60)
                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               <  SRR4302540.1408041/94‑1 (MQ=60)
                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               <  SRR4302540.2054487/94‑1 (MQ=60)
                                                 CACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCA                                              <  SRR4302540.1334929/94‑1 (MQ=60)
                                                 CACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCA                                              <  SRR4302540.1334928/94‑1 (MQ=60)
                                                  ACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCAT                                             <  SRR4302540.2729706/94‑1 (MQ=60)
                                                   CAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATC                                            <  SRR4302540.764939/94‑1 (MQ=60)
                                                    AGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCA                                           <  SRR4302540.8863347/94‑1 (MQ=60)
                                                    AGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCA                                           >  SRR4302540.6932330/1‑94 (MQ=60)
                                                    AGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCA                                           <  SRR4302540.9153362/94‑1 (MQ=60)
                                                    AGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCA                                           <  SRR4302540.9652814/94‑1 (MQ=60)
                                                     GCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAG                                          <  SRR4302540.4492870/94‑1 (MQ=60)
                                                     GCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAG                                          <  SRR4302540.2670063/94‑1 (MQ=60)
                                                     GCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAG                                          <  SRR4302540.3912688/94‑1 (MQ=60)
                                                     GCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAG                                          <  SRR4302540.2365282/94‑1 (MQ=60)
                                                      CACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAACATCATATACCTGTAATCAATTTTCGCTTTCATCAGA                                         <  SRR4302540.1845293/94‑1 (MQ=60)
                                                      CACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGA                                         >  SRR4302540.6628614/1‑94 (MQ=60)
                                                       ACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAA                                        >  SRR4302540.7372500/1‑94 (MQ=60)
                                                        CAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAC                                       <  SRR4302540.7631971/94‑1 (MQ=60)
                                                        CAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAC                                       >  SRR4302540.2025506/1‑94 (MQ=60)
                                                        CAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAC                                       <  SRR4302540.8911614/94‑1 (MQ=60)
                                                        CAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAC                                       <  SRR4302540.8846669/94‑1 (MQ=60)
                                                        CAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAC                                       <  SRR4302540.3539652/94‑1 (MQ=60)
                                                          AGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGT                                     <  SRR4302540.566244/94‑1 (MQ=60)
                                                          ACATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGT                                     <  SRR4302540.7958222/94‑1 (MQ=60)
                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTTCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCC                                   <  SRR4302540.2845154/94‑1 (MQ=60)
                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCG                                   >  SRR4302540.4991936/1‑94 (MQ=60)
                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCC                                   >  SRR4302540.9424260/1‑94 (MQ=60)
                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCC                                   >  SRR4302540.1876329/1‑94 (MQ=60)
                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCC                                   >  SRR4302540.8187466/1‑94 (MQ=60)
                                                             TCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCG                                  >  SRR4302540.5509351/1‑94 (MQ=60)
                                                              CTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGA                                 >  SRR4302540.864897/1‑94 (MQ=60)
                                                                GCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATG                               >  SRR4302540.1062043/1‑94 (MQ=60)
                                                                GCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATG                               <  SRR4302540.4622084/94‑1 (MQ=60)
                                                                GCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATG                               <  SRR4302540.658618/94‑1 (MQ=60)
                                                                 CCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGA                              >  SRR4302540.827418/1‑94 (MQ=60)
                                                                 CCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGA                              >  SRR4302540.827419/1‑94 (MQ=60)
                                                                  CGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAT                             >  SRR4302540.414589/1‑94 (MQ=60)
                                                                  CGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAT                             >  SRR4302540.3066566/1‑94 (MQ=60)
                                                                   GCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATT                            <  SRR4302540.9100763/94‑1 (MQ=60)
                                                                   GCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATT                            >  SRR4302540.3213153/1‑94 (MQ=60)
                                                                    CAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTG                           <  SRR4302540.7892380/94‑1 (MQ=60)
                                                                     AGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGG                          >  SRR4302540.9946213/1‑94 (MQ=60)
                                                                     AGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGG                          >  SRR4302540.7775626/1‑94 (MQ=60)
                                                                     AGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGG                          >  SRR4302540.5240378/1‑94 (MQ=60)
                                                                     AGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGG                          >  SRR4302540.9662050/1‑94 (MQ=60)
                                                                      GCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGT                         >  SRR4302540.3063630/1‑94 (MQ=60)
                                                                      GCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGT                         >  SRR4302540.1541276/1‑94 (MQ=60)
                                                                      GCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGT                         >  SRR4302540.3846789/1‑94 (MQ=60)
                                                                       CCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTT                        >  SRR4302540.8113051/1‑94 (MQ=60)
                                                                        CATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTT                       >  SRR4302540.3162689/1‑94 (MQ=60)
                                                                        CATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTT                       >  SRR4302540.1483209/1‑94 (MQ=60)
                                                                         ATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGCGAAATAATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGTTTTT                      >  SRR4302540.8573649/1‑94 (MQ=60)
                                                                         ATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTT                      >  SRR4302540.6684351/1‑94 (MQ=60)
                                                                         ATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTT                      >  SRR4302540.1371750/1‑94 (MQ=60)
                                                                         ATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTT                      >  SRR4302540.8573645/1‑94 (MQ=60)
                                                                          TTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTT                     <  SRR4302540.10026190/94‑1 (MQ=60)
                                                                          TTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTT                     <  SRR4302540.10095914/94‑1 (MQ=60)
                                                                          TTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTT                     <  SRR4302540.3052919/94‑1 (MQ=60)
                                                                          TTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTT                     >  SRR4302540.5187725/1‑94 (MQ=60)
                                                                           TGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTG                    <  SRR4302540.4033166/94‑1 (MQ=60)
                                                                            GTTATGGCCGCAGTTGCGGGCGAGCGAAGGTGAAAAATCATATACCTGTAATCCATTTTCGCTTTCATCAGAACGTGCGATGATTGGTTGTTGC                   >  SRR4302540.7054383/1‑94 (MQ=60)
                                                                             TTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGNACGTCCGATGATTGGTTTTTGCC                  >  SRR4302540.4377815/1‑94 (MQ=60)
                                                                             TTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCC                  <  SRR4302540.1553126/94‑1 (MQ=60)
                                                                             TTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCC                  <  SRR4302540.5156333/94‑1 (MQ=60)
                                                                             TTATGGCCGCAGTTGCGGGTGAGCGAAGGGGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCC                  >  SRR4302540.5411084/1‑94 (MQ=60)
                                                                              TATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCC                 >  SRR4302540.6345536/1‑94 (MQ=60)
                                                                              TATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCC                 <  SRR4302540.4885559/94‑1 (MQ=60)
                                                                               ATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCG                >  SRR4302540.8082645/1‑94 (MQ=60)
                                                                               ATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCG                <  SRR4302540.10103832/94‑1 (MQ=60)
                                                                               ATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCG                <  SRR4302540.250143/94‑1 (MQ=60)
                                                                               ATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCG                >  SRR4302540.8082351/1‑94 (MQ=60)
                                                                                TGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGG               <  SRR4302540.2149530/94‑1 (MQ=60)
                                                                                  GCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGT             <  SRR4302540.5442869/94‑1 (MQ=60)
                                                                                   CCGCAGTTGCGTGTGAGCGAATGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAGTGGTTTTTGCCCGGGTG            <  SRR4302540.1322253/94‑1 (MQ=60)
                                                                                   CCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTG            >  SRR4302540.9723096/1‑94 (MQ=60)
                                                                                    CGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGG           >  SRR4302540.4866354/1‑94 (MQ=60)
                                                                                    CGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGC           >  SRR4302540.6194642/1‑94 (MQ=60)
                                                                                    CGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGC           <  SRR4302540.7155729/94‑1 (MQ=60)
                                                                                    CGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGC           >  SRR4302540.7202155/1‑94 (MQ=60)
                                                                                    CGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGC           >  SRR4302540.2156081/1‑94 (MQ=60)
                                                                                      CAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGG         >  SRR4302540.1200863/1‑94 (MQ=60)
                                                                                       AGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGA        >  SRR4302540.8709632/1‑94 (MQ=60)
                                                                                       AGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGA        >  SRR4302540.5585911/1‑94 (MQ=60)
                                                                                       AGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGA        >  SRR4302540.4202498/1‑94 (MQ=60)
                                                                                       AGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGA        >  SRR4302540.3986865/1‑94 (MQ=60)
                                                                                        GTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAG       <  SRR4302540.2705249/94‑1 (MQ=60)
                                                                                        GTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAG       >  SRR4302540.2358125/1‑94 (MQ=60)
                                                                                        GTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAG       >  SRR4302540.7890166/1‑94 (MQ=60)
                                                                                          TGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTT     <  SRR4302540.5534142/94‑1 (MQ=60)
                                                                                            CGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTG   <  SRR4302540.3437238/94‑1 (MQ=60)
                                                                                            CGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTG   <  SRR4302540.629218/94‑1 (MQ=60)
                                                                                            CGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTG   <  SRR4302540.8218126/94‑1 (MQ=60)
                                                                                             GGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGG  >  SRR4302540.9628954/1‑94 (MQ=60)
                                                                                             GGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGG  >  SRR4302540.3747991/1‑94 (MQ=60)
                                                                                             GGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGG  <  SRR4302540.7130667/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGG  >  NC_000913/3792944‑3793130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑