Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,814,809 A→G K169K (AAA→AAG)  yicC → putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,814,8090AG99.8% 1561.2 / ‑5.4 479K169K (AAA→AAG) yicCputative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (242/236);  total (243/236)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 489 total aligned reads displayed.

CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAA  >  NC_000913/3814719‑3814898
                                                                                          |                                                                                         
cgacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGCAGTGGTCAAGGt                                                                                         >  1:7104428/1‑93 (MQ=255)
cgacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGt                                                                                         >  1:2716839/1‑93 (MQ=255)
 gacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTc                                                                                        <  1:8951527/93‑1 (MQ=255)
 gacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTc                                                                                        <  2:8413678/93‑1 (MQ=255)
  acTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTcc                                                                                       >  1:6651300/1‑93 (MQ=255)
     ttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGcc                                                                                    >  1:4549544/1‑92 (MQ=255)
     ttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCt                                                                                    >  1:1483541/1‑93 (MQ=255)
      tATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTc                                                                                   <  1:1166736/93‑1 (MQ=255)
       aTTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTcc                                                                                  >  1:6196943/1‑93 (MQ=255)
       aTTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTcc                                                                                  >  1:3255038/1‑93 (MQ=255)
        ttGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTccc                                                                                 >  1:8262628/1‑93 (MQ=255)
         tGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCa                                                                                <  2:9684732/93‑1 (MQ=255)
         tGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCa                                                                                <  2:4988435/93‑1 (MQ=255)
          ggcgcgcgcgAAACCGAAGGTCGGGCACTGAAAGCATTGATCGAGCAGCGTCGGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCat                                                                               <  1:7900251/91‑1 (MQ=255)
          gTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCat                                                                               <  1:8354954/93‑1 (MQ=255)
           tCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCata                                                                              <  1:8514338/93‑1 (MQ=255)
           tCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCata                                                                              <  2:9774349/93‑1 (MQ=255)
           tCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCata                                                                              <  2:3985017/93‑1 (MQ=255)
           tCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCata                                                                              >  2:3416872/1‑93 (MQ=255)
              cgcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGc                                                                           >  2:6750615/1‑93 (MQ=255)
               gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGcc                                                                          <  2:5725416/93‑1 (MQ=255)
               gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGcc                                                                          <  2:9167141/93‑1 (MQ=255)
               gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGcc                                                                          >  2:856618/1‑93 (MQ=255)
                cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCg                                                                         >  1:905365/1‑93 (MQ=255)
                   gAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGaa                                                                      >  2:2242791/1‑93 (MQ=255)
                   gAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGaa                                                                      >  1:8031680/1‑93 (MQ=255)
                   gAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGaa                                                                      <  2:781832/93‑1 (MQ=255)
                   caaaCCGAAGGTCAGGCCCTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCGCCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGaa                                                                      <  2:8230033/92‑1 (MQ=255)
                     aaCCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAAt                                                                    >  1:6439094/1‑93 (MQ=255)
                      aCCGAACGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATc                                                                   <  1:7456497/93‑1 (MQ=255)
                       ccGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATcc                                                                  >  2:3098011/1‑93 (MQ=255)
                        cGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCt                                                                 >  1:3593364/1‑93 (MQ=255)
                         gAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTg                                                                >  2:5274785/1‑93 (MQ=255)
                           aGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCa                                                              >  2:9000978/1‑93 (MQ=255)
                             gTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAAt                                                            <  1:9342468/93‑1 (MQ=255)
                             gTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAAt                                                            <  2:5123346/93‑1 (MQ=255)
                               cAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATgg                                                          <  1:5531541/93‑1 (MQ=255)
                               cAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATgg                                                          <  1:2831430/93‑1 (MQ=255)
                                aGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGc                                                         <  1:1916182/93‑1 (MQ=255)
                                 ggCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCa                                                        >  1:8671372/1‑93 (MQ=255)
                                 ggCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCa                                                        <  1:7367209/93‑1 (MQ=255)
                                  gCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAg                                                       <  1:9420053/93‑1 (MQ=255)
                                     cTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGt                                                    >  2:9927280/1‑93 (MQ=255)
                                      tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCTGGAAATCCTGCAATGGCAGCGTg                                                   >  2:5527743/1‑93 (MQ=255)
                                      tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                                                   >  2:7951386/1‑93 (MQ=255)
                                       gAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGa                                                  >  1:5780893/1‑93 (MQ=255)
                                       gAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGa                                                  <  2:2628518/93‑1 (MQ=255)
                                        aaaGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAg                                                 <  2:6974092/93‑1 (MQ=255)
                                         aaGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGc                                                >  2:9294740/1‑93 (MQ=255)
                                         aaGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGc                                                <  2:188122/93‑1 (MQ=255)
                                            cATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTc                                             <  1:5105128/93‑1 (MQ=255)
                                              ttGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTg                                           >  1:6270613/1‑93 (MQ=255)
                                               tGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTgg                                          >  1:6093606/1‑93 (MQ=255)
                                                gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGt                                         <  2:6769051/93‑1 (MQ=255)
                                                 aTCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTc                                        <  2:1756069/93‑1 (MQ=255)
                                                   cGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTcgc                                      >  2:8823545/1‑93 (MQ=255)
                                                      gcagcGTCTGGAAGGCGTCACCGCCGAAGTGTTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGaa                                   <  2:354039/93‑1 (MQ=255)
                                                      gcagcGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGaa                                   <  2:7258839/93‑1 (MQ=255)
                                                         gcGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGCGCGTCTGGTCGCGGAGCt                                >  2:2511915/1‑93 (MQ=255)
                                                           gTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTgg                              <  1:1003160/93‑1 (MQ=255)
                                                           gTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTgg                              >  1:9141125/1‑93 (MQ=255)
                                                            tCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGa                             >  2:362545/1‑93 (MQ=255)
                                                            tCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGCCGCGAAGCTGGa                             >  1:8782600/1‑93 (MQ=255)
                                                             cTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGaa                            <  1:3172680/93‑1 (MQ=255)
                                                             cTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGaa                            <  1:3942246/93‑1 (MQ=255)
                                                              tGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAg                           >  2:4465713/1‑93 (MQ=255)
                                                               ggAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGa                          >  2:1368109/1‑93 (MQ=255)
                                                               ggAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGa                          >  1:4377040/1‑93 (MQ=255)
                                                               ggAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGa                          >  1:9206640/1‑93 (MQ=255)
                                                               ggAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGa                          >  1:3813101/1‑93 (MQ=255)
                                                                gAAGGGGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGAt                         <  2:628839/93‑1 (MQ=255)
                                                                gAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGAt                         <  2:2065012/93‑1 (MQ=255)
                                                                 aaGGGGTCACCGCGGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAAATg                        <  1:7626615/93‑1 (MQ=255)
                                                                 aaGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATg                        >  1:4973800/1‑93 (MQ=255)
                                                                 aaGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATg                        >  2:2971345/1‑93 (MQ=255)
                                                                   ggCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCt                      >  1:7056772/1‑93 (MQ=255)
                                                                   ggCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCt                      <  1:996094/93‑1 (MQ=255)
                                                                   ggCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCt                      >  1:2171560/1‑93 (MQ=255)
                                                                    gCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTc                     <  1:4655586/93‑1 (MQ=255)
                                                                    gCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTc                     <  1:4500905/93‑1 (MQ=255)
                                                                    gCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTc                     <  2:3705560/93‑1 (MQ=255)
                                                                    gCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTc                     <  1:1652051/93‑1 (MQ=255)
                                                                     gcgcACCGCCGAAGTGGTCAAGGTCCGCTCCTATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCa                    <  1:6902514/90‑1 (MQ=255)
                                                                      gTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAg                   >  2:6097824/1‑93 (MQ=255)
                                                                       tCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAgg                  <  2:1589952/93‑1 (MQ=255)
                                                                         aCCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTg                >  1:9629369/1‑93 (MQ=255)
                                                                          ccgccgAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGc               <  2:6527576/93‑1 (MQ=255)
                                                                             ccgAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAAc            <  1:5940043/93‑1 (MQ=255)
                                                                             ccgAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAAc            <  1:2024389/93‑1 (MQ=255)
                                                                              cgAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACt           >  1:24388/1‑93 (MQ=255)
                                                                               gAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTg          <  1:4158421/93‑1 (MQ=255)
                                                                                aaGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTgg         >  1:9867508/1‑93 (MQ=255)
                                                                                aaGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTgg         <  2:5847597/93‑1 (MQ=255)
                                                                                 aGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGa        <  1:5668964/93‑1 (MQ=255)
                                                                                 aGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATACTCAGGTGCAACTGGa        <  1:5878240/93‑1 (MQ=255)
                                                                                  gTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGaa       >  2:3895080/1‑93 (MQ=255)
                                                                                    ggTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGaaaa     <  2:4723408/93‑1 (MQ=255)
                                                                                     gTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAaac    >  2:1084870/1‑93 (MQ=255)
                                                                                      tCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAaaca   >  1:3018250/1‑93 (MQ=255)
                                                                                      tCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAaaca   >  1:7771334/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAA  >  NC_000913/3814719‑3814898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 288 total aligned reads displayed.
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGT  >  NC_000913/3814716‑3814902
                                                                                             |                                                                                             
GGACGACTTTATTGTCGCGCGCGAAACGGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                               <  SRR4302540.7756435/94‑1 (MQ=60)
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGATCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                               <  SRR4302540.3447156/94‑1 (MQ=60)
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                               <  SRR4302540.81619/94‑1 (MQ=60)
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                               >  SRR4302540.2356612/1‑94 (MQ=60)
  ACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGCAGTGGTCAAGGT                                                                                             >  SRR4302540.7183474/1‑94 (MQ=60)
  ACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGT                                                                                             >  SRR4302540.2746136/1‑94 (MQ=60)
  ACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCGGGAAGGCGTCACCGCCGAAGTGGTCAAGGT                                                                                             <  SRR4302540.4349993/94‑1 (MQ=60)
   CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTC                                                                                            >  SRR4302540.7956308/1‑94 (MQ=60)
    GACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCC                                                                                           <  SRR4302540.6201168/94‑1 (MQ=60)
    GACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCC                                                                                           >  SRR4302540.4623164/1‑94 (MQ=60)
     ACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCG                                                                                          >  SRR4302540.7654342/1‑94 (MQ=60)
     ACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCG                                                                                          >  SRR4302540.9347479/1‑94 (MQ=60)
      CTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGC                                                                                         >  SRR4302540.3844719/1‑94 (MQ=60)
       TTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCT                                                                                        <  SRR4302540.5028437/94‑1 (MQ=60)
         TATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCC                                                                                      >  SRR4302540.6265931/1‑94 (MQ=60)
         TATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCC                                                                                      <  SRR4302540.1178832/94‑1 (MQ=60)
         TATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCC                                                                                      >  SRR4302540.4030827/1‑94 (MQ=60)
         TATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCC                                                                                      >  SRR4302540.3290518/1‑94 (MQ=60)
          ATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCC                                                                                     >  SRR4302540.8358645/1‑94 (MQ=60)
          ATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCC                                                                                     >  SRR4302540.1038696/1‑94 (MQ=60)
          ATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCC                                                                                     <  SRR4302540.7960569/94‑1 (MQ=60)
            TGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCAT                                                                                   >  SRR4302540.3876616/1‑94 (MQ=60)
            TGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCAT                                                                                   >  SRR4302540.9926160/1‑94 (MQ=60)
             tgcccgccCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCCAGCCGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATA                                                                                  <  SRR4302540.8451925/86‑1 (MQ=60)
             GTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATA                                                                                  >  SRR4302540.2156031/1‑94 (MQ=60)
             GTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATA                                                                                  <  SRR4302540.5880655/94‑1 (MQ=60)
             GTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATA                                                                                  <  SRR4302540.5880656/94‑1 (MQ=60)
             GGCGCGCGCGAAACCGAAGGTCGGGCACTGAAAGCATTGATCGAGCAGCGTCGGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATA                                                                                  <  SRR4302540.7992399/94‑1 (MQ=60)
              TCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATAT                                                                                 <  SRR4302540.8613293/94‑1 (MQ=60)
              TCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATAT                                                                                 <  SRR4302540.889478/94‑1 (MQ=60)
               CGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATG                                                                                <  SRR4302540.8775228/94‑1 (MQ=60)
                  GCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCG                                                                             >  SRR4302540.6346200/1‑94 (MQ=60)
                  GCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCG                                                                             >  SRR4302540.914973/1‑94 (MQ=60)
                   CGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCGCCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGG                                                                            <  SRR4302540.1815737/94‑1 (MQ=60)
                     CGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAA                                                                          >  SRR4302540.8125058/1‑94 (MQ=60)
                     CGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAA                                                                          <  SRR4302540.8412684/94‑1 (MQ=60)
                     CGAAACCGAAGGGCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTAGTCAAGGTCCGCTCCCATATGCCGGAA                                                                          <  SRR4302540.8412685/94‑1 (MQ=60)
                      GAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAA                                                                         >  SRR4302540.6517293/1‑94 (MQ=60)
                       AAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAAT                                                                        >  SRR4302540.3886294/1‑94 (MQ=60)
                       AAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAAT                                                                        <  SRR4302540.9585343/94‑1 (MQ=60)
                       AAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAAT                                                                        >  SRR4302540.6510756/1‑94 (MQ=60)
                        AACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATC                                                                       >  SRR4302540.9533724/1‑94 (MQ=60)
                         ACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCC                                                                      <  SRR4302540.9142346/94‑1 (MQ=60)
                         ACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCC                                                                      <  SRR4302540.293924/94‑1 (MQ=60)
                         ACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCC                                                                      >  SRR4302540.3557272/1‑94 (MQ=60)
                         ACCGAACGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCC                                                                      <  SRR4302540.7539633/94‑1 (MQ=60)
                          CCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCT                                                                     >  SRR4302540.3632616/1‑94 (MQ=60)
                          CCGAAGGTCAGGCACTGAAAGCATTGAGCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCT                                                                     <  SRR4302540.1371662/94‑1 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                    >  SRR4302540.5961944/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                    >  SRR4302540.4452917/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                    >  SRR4302540.4212503/1‑94 (MQ=60)
                              AGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAA                                                                 >  SRR4302540.5400087/1‑94 (MQ=60)
                              AGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAA                                                                 <  SRR4302540.6203510/94‑1 (MQ=60)
                              AGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAA                                                                 >  SRR4302540.8118254/1‑94 (MQ=60)
                               ttTCAGGCACTGAAACCATTTATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAAT                                                                <  SRR4302540.2633696/92‑1 (MQ=60)
                               GGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAAT                                                                <  SRR4302540.1545480/94‑1 (MQ=60)
                                GTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATG                                                               <  SRR4302540.4403983/94‑1 (MQ=60)
                                GTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATG                                                               <  SRR4302540.6952419/94‑1 (MQ=60)
                                GTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATG                                                               <  SRR4302540.9453787/94‑1 (MQ=60)
                                GTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGCCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATG                                                               >  SRR4302540.5782352/1‑94 (MQ=60)
                                 GCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGG                                                              <  SRR4302540.9469602/94‑1 (MQ=60)
                                  CAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGC                                                             <  SRR4302540.2862054/94‑1 (MQ=60)
                                  CAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGC                                                             <  SRR4302540.5592462/94‑1 (MQ=60)
                                   AGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCA                                                            <  SRR4302540.4350177/94‑1 (MQ=60)
                                   AGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCA                                                            <  SRR4302540.7835402/94‑1 (MQ=60)
                                   AGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCA                                                            >  SRR4302540.8772097/1‑94 (MQ=60)
                                   AGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCA                                                            <  SRR4302540.1937094/94‑1 (MQ=60)
                                   AGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCA                                                            <  SRR4302540.8178014/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                           <  SRR4302540.5686360/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                           <  SRR4302540.7488173/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                           <  SRR4302540.5130917/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                           <  SRR4302540.4554816/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGAAG                                                           >  SRR4302540.2034684/1‑94 (MQ=60)
                                     GCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGC                                                          >  SRR4302540.6501238/1‑94 (MQ=60)
                                     GCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGC                                                          <  SRR4302540.7854870/94‑1 (MQ=60)
                                       ACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGT                                                        <  SRR4302540.2398754/94‑1 (MQ=60)
                                        CTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTG                                                       >  SRR4302540.9923947/1‑94 (MQ=60)
                                        CTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTG                                                       >  SRR4302540.5052280/1‑94 (MQ=60)
                                        CTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTG                                                       >  SRR4302540.6381058/1‑94 (MQ=60)
                                         TGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGGAATCCTGCAATGGCAGCGTGA                                                      >  SRR4302540.2396124/1‑94 (MQ=60)
                                         TGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGA                                                      >  SRR4302540.5844753/1‑94 (MQ=60)
                                         TGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGA                                                      >  SRR4302540.2786119/1‑94 (MQ=60)
                                          GAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAG                                                     >  SRR4302540.5961500/1‑94 (MQ=60)
                                           AAAACATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGC                                                    <  SRR4302540.3279684/94‑1 (MQ=60)
                                             AGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGT                                                  <  SRR4302540.7056562/94‑1 (MQ=60)
                                             AGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGT                                                  >  SRR4302540.3150942/1‑94 (MQ=60)
                                              GCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTC                                                 >  SRR4302540.6188430/1‑94 (MQ=60)
                                              GCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTC                                                 >  SRR4302540.26879/1‑94 (MQ=60)
                                               CATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCT                                                <  SRR4302540.5160395/94‑1 (MQ=60)
                                               CATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCT                                                <  SRR4302540.7038564/94‑1 (MQ=60)
                                               CATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCT                                                <  SRR4302540.4076267/94‑1 (MQ=60)
                                                CTTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTG                                               <  SRR4302540.8706150/94‑1 (MQ=60)
                                                ATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTG                                               >  SRR4302540.1428176/1‑94 (MQ=60)
                                                ATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTG                                               <  SRR4302540.1518038/94‑1 (MQ=60)
                                                 TTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG                                              >  SRR4302540.7049698/1‑94 (MQ=60)
                                                 TTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG                                              <  SRR4302540.6268343/94‑1 (MQ=60)
                                                  TGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGT                                             >  SRR4302540.5838547/1‑94 (MQ=60)
                                                  TGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGT                                             <  SRR4302540.3207658/94‑1 (MQ=60)
                                                   TATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTC                                            <  SRR4302540.8610284/94‑1 (MQ=60)
                                                   GATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTC                                            <  SRR4302540.7583849/94‑1 (MQ=60)
                                                   GATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTC                                            <  SRR4302540.1883158/94‑1 (MQ=60)
                                                        AGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAA                                       >  SRR4302540.4533478/1‑94 (MQ=60)
                                                         GCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAG                                      <  SRR4302540.4649472/94‑1 (MQ=60)
                                                         GCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAG                                      <  SRR4302540.5987301/94‑1 (MQ=60)
                                                         GCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAG                                      <  SRR4302540.9466146/94‑1 (MQ=60)
                                                          CAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGC                                     >  SRR4302540.10024614/1‑94 (MQ=60)
                                                          CAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGC                                     >  SRR4302540.9978521/1‑94 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                    <  SRR4302540.3592929/94‑1 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                    >  SRR4302540.4238072/1‑94 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                    <  SRR4302540.2398986/94‑1 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                    <  SRR4302540.5445884/94‑1 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                    <  SRR4302540.5731613/94‑1 (MQ=60)
                                                            GCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTG                                   >  SRR4302540.7512013/1‑94 (MQ=60)
                                                             CGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGG                                  >  SRR4302540.3775370/1‑94 (MQ=60)
                                                             CGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGG                                  >  SRR4302540.9250945/1‑94 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA                                 >  SRR4302540.1173985/1‑94 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA                                 <  SRR4302540.9291735/94‑1 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA                                 >  SRR4302540.6631581/1‑94 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA                                 <  SRR4302540.3119726/94‑1 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA                                 >  SRR4302540.9746493/1‑94 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA                                 <  SRR4302540.1013344/94‑1 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGCCGCGAAGCTGGA                                 >  SRR4302540.8884269/1‑94 (MQ=60)
                                                               TCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAA                                >  SRR4302540.9511976/1‑94 (MQ=60)
                                                               TCTGGAAGGCGGCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAA                                <  SRR4302540.8347862/94‑1 (MQ=60)
                                                                CTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAG                               <  SRR4302540.7136700/94‑1 (MQ=60)
                                                                CTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAG                               <  SRR4302540.3984829/94‑1 (MQ=60)
                                                                CTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAG                               <  SRR4302540.7136977/94‑1 (MQ=60)
                                                                CTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAG                               >  SRR4302540.4640448/1‑94 (MQ=60)
                                                                CTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAG                               >  SRR4302540.6304556/1‑94 (MQ=60)
                                                                CTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAG                               <  SRR4302540.4730902/94‑1 (MQ=60)
                                                                 TGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGA                              >  SRR4302540.9317116/1‑94 (MQ=60)
                                                                 TGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGA                              >  SRR4302540.3854622/1‑94 (MQ=60)
                                                                  GGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGAT                             >  SRR4302540.9419273/1‑94 (MQ=60)
                                                                  GGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGAT                             <  SRR4302540.3940341/94‑1 (MQ=60)
                                                                  GGAAGGCGTCACCGCCGAAGTGGGCAAGGTCCGCTCCCATATGCCGGAAAACCTGCAATGGCAGCGTGAGCGTCTGTTCGCGAAGCTGTAAGAG                             >  SRR4302540.9094264/1‑94 (MQ=60)
                                                                  GGAAGGCGTCACAGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGAT                             <  SRR4302540.4727751/94‑1 (MQ=60)
                                                                   GAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATG                            <  SRR4302540.8991942/94‑1 (MQ=60)
                                                                   GAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATG                            >  SRR4302540.5027624/1‑94 (MQ=60)
                                                                    AAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGC                           <  SRR4302540.6764262/94‑1 (MQ=60)
                                                                    AAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGC                           >  SRR4302540.6657649/1‑94 (MQ=60)
                                                                    AAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGC                           <  SRR4302540.7712388/94‑1 (MQ=60)
                                                                    AAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGC                           >  SRR4302540.2018371/1‑94 (MQ=60)
                                                                     AGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          >  SRR4302540.1450353/1‑94 (MQ=60)
                                                                     AGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          <  SRR4302540.2799956/94‑1 (MQ=60)
                                                                     AGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          >  SRR4302540.2194975/1‑94 (MQ=60)
                                                                     AGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          <  SRR4302540.7444373/94‑1 (MQ=60)
                                                                     AGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          >  SRR4302540.7428351/1‑94 (MQ=60)
                                                                     AGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          <  SRR4302540.9194959/94‑1 (MQ=60)
                                                                     AGGCGCCACTGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          <  SRR4302540.330364/94‑1 (MQ=60)
                                                                     AGCCGTCGCCGCCGAAGTGGTCAAGGTCCGATCCCATATGCAGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                          <  SRR4302540.9194958/94‑1 (MQ=60)
                                                                      GGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTC                         <  SRR4302540.1006234/94‑1 (MQ=60)
                                                                       GCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCA                        <  SRR4302540.4705407/94‑1 (MQ=60)
                                                                       GCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCA                        <  SRR4302540.1670061/94‑1 (MQ=60)
                                                                       GCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCA                        >  SRR4302540.5696403/1‑94 (MQ=60)
                                                                       GCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCA                        >  SRR4302540.5696149/1‑94 (MQ=60)
                                                                       GCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCA                        <  SRR4302540.4705408/94‑1 (MQ=60)
                                                                       GCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCA                        <  SRR4302540.4549696/94‑1 (MQ=60)
                                                                       GCG‑CACCGCCGAAGTGGTCAAGGTCCGCTCCTATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAG                       <  SRR4302540.6979244/94‑1 (MQ=60)
                                                                        CGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAG                       <  SRR4302540.6794077/94‑1 (MQ=60)
                                                                        CGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAG                       <  SRR4302540.2822968/94‑1 (MQ=60)
                                                                        CGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAG                       <  SRR4302540.7295987/94‑1 (MQ=60)
                                                                        CGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAG                       <  SRR4302540.9259483/94‑1 (MQ=60)
                                                                         GTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGG                      >  SRR4302540.6093595/1‑94 (MQ=60)
                                                                         GTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGG                      >  SRR4302540.9576438/1‑94 (MQ=60)
                                                                         GTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGG                      >  SRR4302540.4486383/1‑94 (MQ=60)
                                                                         GTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGG                      <  SRR4302540.8778822/94‑1 (MQ=60)
                                                                           CACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTG                    >  SRR4302540.9742840/1‑94 (MQ=60)
                                                                           CACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTG                    >  SRR4302540.3222754/1‑94 (MQ=60)
                                                                            ACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGC                   >  SRR4302540.4573414/1‑94 (MQ=60)
                                                                             CCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCA                  >  SRR4302540.7754172/1‑94 (MQ=60)
                                                                              CGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAA                 >  SRR4302540.8469102/1‑94 (MQ=60)
                                                                              CGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGGCGCGAAGCTGGAAGATGCTCAGGTGCAA                 >  SRR4302540.3179697/1‑94 (MQ=60)
                                                                               GCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAAC                <  SRR4302540.2123038/94‑1 (MQ=60)
                                                                               GCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAAC                >  SRR4302540.6061904/1‑94 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT               <  SRR4302540.10018599/94‑1 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT               >  SRR4302540.8735090/1‑94 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT               <  SRR4302540.2046262/94‑1 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT               >  SRR4302540.24659/1‑94 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT               <  SRR4302540.3014968/94‑1 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT               <  SRR4302540.7140663/94‑1 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT               <  SRR4302540.6005710/94‑1 (MQ=60)
                                                                                  GAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGG             >  SRR4302540.9982765/1‑94 (MQ=60)
                                                                                   AAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGA            >  SRR4302540.904084/1‑94 (MQ=60)
                                                                                   AAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGA            >  SRR4302540.1939337/1‑94 (MQ=60)
                                                                                   AAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGA            >  SRR4302540.6287102/1‑94 (MQ=60)
                                                                                    AGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAA           >  SRR4302540.9112344/1‑94 (MQ=60)
                                                                                    AGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAA           >  SRR4302540.1624731/1‑94 (MQ=60)
                                                                                    AGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATACTCAGGTGCAACTGGAA           <  SRR4302540.5943214/94‑1 (MQ=60)
                                                                                      TGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGCAGCTGGACGATGCTCAGGTGCAACTGGAAAA         >  SRR4302540.4054680/1‑94 (MQ=60)
                                                                                       GGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAAC        >  SRR4302540.8434095/1‑94 (MQ=60)
                                                                                       GGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAAC        >  SRR4302540.3635487/1‑94 (MQ=60)
                                                                                       GGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAAC        >  SRR4302540.9172026/1‑94 (MQ=60)
                                                                                        GTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACA       >  SRR4302540.7862187/1‑94 (MQ=60)
                                                                                        GTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACA       >  SRR4302540.3051426/1‑94 (MQ=60)
                                                                                          CAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAAC     <  SRR4302540.8096045/94‑1 (MQ=60)
                                                                                          CAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAAC     <  SRR4302540.7993997/94‑1 (MQ=60)
                                                                                          CAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAAC     >  SRR4302540.2079470/1‑94 (MQ=60)
                                                                                           AAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACC    <  SRR4302540.5568128/94‑1 (MQ=60)
                                                                                           AAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACC    <  SRR4302540.2635685/94‑1 (MQ=60)
                                                                                           AAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACC    <  SRR4302540.9403595/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGT  >  NC_000913/3814716‑3814902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑