Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,814,809 A→G K169K (AAA→AAG)  yicC → putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,814,8090AG100.0% 775.5 / NA 238K169K (AAA→AAG) yicCputative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (129/109);  total (129/109)

Only 100 of 244 total aligned reads displayed.

GACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAA  >  NC_000913/3814720‑3814898
                                                                                         |                                                                                         
gacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTc                                                                                        <  2:2682989/93‑1 (MQ=255)
   tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGc                                                                                     >  1:1233756/1‑93 (MQ=255)
   tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGc                                                                                     <  2:1171706/93‑1 (MQ=255)
   tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGc                                                                                     >  1:3836367/1‑93 (MQ=255)
   tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGc                                                                                     >  2:4172167/1‑93 (MQ=255)
     tATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTc                                                                                   >  2:122666/1‑93 (MQ=255)
       ttGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTccc                                                                                 >  1:6118753/1‑93 (MQ=255)
       ttGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTccc                                                                                 >  1:1040488/1‑93 (MQ=255)
            gcgcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATg                                                                            >  2:4795724/1‑93 (MQ=255)
            gcgcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATg                                                                            <  1:6039654/93‑1 (MQ=255)
            ccgcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATg                                                                            <  2:2405348/92‑1 (MQ=255)
              gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGcc                                                                          <  1:608258/93‑1 (MQ=255)
              gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGcc                                                                          <  1:5292220/93‑1 (MQ=255)
               cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCg                                                                         <  2:1325564/93‑1 (MQ=255)
               cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCg                                                                         >  1:1657244/1‑93 (MQ=255)
                 cgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGa                                                                       >  2:2115336/1‑93 (MQ=255)
                   aaaCCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGaaa                                                                     >  2:5580152/1‑93 (MQ=255)
                   aaaCCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGaaa                                                                     >  1:1726230/1‑93 (MQ=255)
                     aCCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATc                                                                   <  2:4685992/93‑1 (MQ=255)
                      ccGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATcc                                                                  <  2:4462717/93‑1 (MQ=255)
                      ccGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATcc                                                                  >  1:5772618/1‑93 (MQ=255)
                       cGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCt                                                                 <  2:2751430/93‑1 (MQ=255)
                       cGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCt                                                                 <  1:4008476/93‑1 (MQ=255)
                       cGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCt                                                                 >  2:2977847/1‑93 (MQ=255)
                        gAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTg                                                                >  1:5642664/1‑93 (MQ=255)
                        gAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTg                                                                >  1:118818/1‑93 (MQ=255)
                        gAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTg                                                                >  1:4756343/1‑93 (MQ=255)
                        gAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTg                                                                >  1:1293381/1‑93 (MQ=255)
                         aaGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGc                                                               >  2:2255600/1‑93 (MQ=255)
                          aGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCa                                                              <  1:3728854/93‑1 (MQ=255)
                            gTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAAt                                                            <  2:3921280/93‑1 (MQ=255)
                             tCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATg                                                           >  1:4590715/1‑93 (MQ=255)
                              cAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATgg                                                          >  1:5512081/1‑93 (MQ=255)
                               aGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGc                                                         <  2:2353965/93‑1 (MQ=255)
                                ggCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCa                                                        <  1:3054465/93‑1 (MQ=255)
                                 gCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAg                                                       <  2:5118421/93‑1 (MQ=255)
                                  cACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGc                                                      >  2:1644462/1‑93 (MQ=255)
                                  cACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGc                                                      >  1:2179848/1‑93 (MQ=255)
                                   aCTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCg                                                     >  2:3629221/1‑93 (MQ=255)
                                   aCTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCg                                                     >  2:3288135/1‑93 (MQ=255)
                                   aCTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCg                                                     >  2:203872/1‑93 (MQ=255)
                                    cTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCgg                                                    >  2:833721/1‑92 (MQ=255)
                                     tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                                                   >  1:788227/1‑93 (MQ=255)
                                     tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                                                   <  2:583124/93‑1 (MQ=255)
                                     tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                                                   <  1:4885013/93‑1 (MQ=255)
                                     tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                                                   >  2:3264700/1‑93 (MQ=255)
                                     tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                                                   >  2:4624377/1‑93 (MQ=255)
                                     tGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                                                   >  1:5305288/1‑93 (MQ=255)
                                      gAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGa                                                  <  2:6060758/93‑1 (MQ=255)
                                       aaaGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAg                                                 >  2:3942512/1‑93 (MQ=255)
                                       aaaGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAg                                                 <  2:5954511/93‑1 (MQ=255)
                                       aaaGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAg                                                 >  2:2303640/1‑93 (MQ=255)
                                         aGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCg                                               >  2:5311881/1‑93 (MQ=255)
                                           cATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTc                                             >  1:710254/1‑93 (MQ=255)
                                           cATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTc                                             <  1:2990284/93‑1 (MQ=255)
                                            aTTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCt                                            >  2:1985984/1‑93 (MQ=255)
                                            aTTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCt                                            >  1:6203652/1‑93 (MQ=255)
                                             ttGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGACTg                                           >  2:5347579/1‑93 (MQ=255)
                                              tGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTgg                                          <  1:4828316/93‑1 (MQ=255)
                                               gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGt                                         >  1:638148/1‑93 (MQ=255)
                                               gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGt                                         <  1:932902/93‑1 (MQ=255)
                                               gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGt                                         <  2:4304049/93‑1 (MQ=255)
                                                aTCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTc                                        <  2:579001/93‑1 (MQ=255)
                                                 tCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTcg                                       <  2:1712804/93‑1 (MQ=255)
                                                 tCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGGcg                                       >  1:371268/1‑93 (MQ=255)
                                                  cGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTcgc                                      <  1:5240171/93‑1 (MQ=255)
                                                    agcagcGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGGCGCGa                                    >  2:322061/1‑93 (MQ=255)
                                                     gcagcGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGaa                                   <  1:2037407/93‑1 (MQ=255)
                                                      cagcGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTATGGTCGCGAAg                                  >  1:3349866/1‑93 (MQ=255)
                                                       agcGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGc                                 <  1:2675629/93‑1 (MQ=255)
                                                       agcGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGc                                 <  1:3588753/93‑1 (MQ=255)
                                                         cGTCTGGAAGGCGGCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTg                               <  2:3810708/93‑1 (MQ=255)
                                                          gTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTgg                              >  1:4312783/1‑93 (MQ=255)
                                                           tCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGa                             >  2:848364/1‑93 (MQ=255)
                                                           tCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGa                             >  2:3390926/1‑93 (MQ=255)
                                                           tCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGa                             >  2:189409/1‑93 (MQ=255)
                                                            cTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGaa                            <  2:6289323/93‑1 (MQ=255)
                                                            cTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGaa                            <  2:4403108/93‑1 (MQ=255)
                                                              ggAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGa                          <  1:5464534/93‑1 (MQ=255)
                                                                 aGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGc                       <  2:5066198/93‑1 (MQ=255)
                                                                  ggCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCt                      >  1:3444978/1‑93 (MQ=255)
                                                                  ggCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCt                      <  2:4574910/93‑1 (MQ=255)
                                                                   gCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTAAGCGTCTGGTCGCGAAGCTGGAAGATGCTc                     <  2:172628/93‑1 (MQ=255)
                                                                    cGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCa                    <  1:2808526/93‑1 (MQ=255)
                                                                     gTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAg                   >  1:5928496/1‑93 (MQ=255)
                                                                      tCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAgg                  >  1:45370/1‑93 (MQ=255)
                                                                      tCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAgg                  >  2:372876/1‑93 (MQ=255)
                                                                       cACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGt                 <  2:5517613/93‑1 (MQ=255)
                                                                       cACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGt                 <  2:5780958/93‑1 (MQ=255)
                                                                        aCCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTg                >  2:708811/1‑93 (MQ=255)
                                                                          cgccgAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCa              >  2:2578880/1‑93 (MQ=255)
                                                                             cgAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACt           <  1:2247497/93‑1 (MQ=255)
                                                                             cgAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACt           >  1:1448259/1‑93 (MQ=255)
                                                                              gAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTg          >  2:2651101/1‑93 (MQ=255)
                                                                              gAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTg          <  2:4216300/93‑1 (MQ=255)
                                                                               aaGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTgg         >  1:4649713/1‑93 (MQ=255)
                                                                                  tGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGaaa      <  1:5393058/93‑1 (MQ=255)
                                                                                    gTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAaac    <  2:3132390/93‑1 (MQ=255)
                                                                                     tCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAaaca   >  2:1015838/1‑93 (MQ=255)
                                                                                      cAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAaacaa  <  1:5120273/93‑1 (MQ=255)
                                                                                         |                                                                                         
GACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAA  >  NC_000913/3814720‑3814898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACC  >  NC_000913/3814716‑3814900
                                                                                             |                                                                                           
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                             <  SRR4302533.3838394/94‑1 (MQ=60)
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                             >  SRR4302533.4776390/1‑94 (MQ=60)
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                             >  SRR4302533.5422598/1‑94 (MQ=60)
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAG                                                                                             >  SRR4302533.6316053/1‑94 (MQ=60)
 GACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGG                                                                                            <  SRR4302533.1050767/94‑1 (MQ=60)
  GCGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGT                                                                                           <  SRR4302533.4793448/94‑1 (MQ=60)
   CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTC                                                                                          >  SRR4302533.6084660/1‑94 (MQ=60)
   CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTC                                                                                          >  SRR4302533.5025643/1‑94 (MQ=60)
    GACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCC                                                                                         <  SRR4302533.8597/94‑1 (MQ=60)
      CTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGC                                                                                       >  SRR4302533.1241136/1‑94 (MQ=60)
      CTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGC                                                                                       <  SRR4302533.4906143/94‑1 (MQ=60)
      CTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGC                                                                                       >  SRR4302533.3859808/1‑94 (MQ=60)
        TTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTC                                                                                     >  SRR4302533.612916/1‑94 (MQ=60)
          ATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCC                                                                                   >  SRR4302533.1046606/1‑94 (MQ=60)
          ATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCC                                                                                   <  SRR4302533.1660168/94‑1 (MQ=60)
          ATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCC                                                                                   >  SRR4302533.6155655/1‑94 (MQ=60)
            TGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCAT                                                                                 <  SRR4302533.5464356/94‑1 (MQ=60)
            TGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCAT                                                                                 <  SRR4302533.6075607/94‑1 (MQ=60)
              TCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATAT                                                                               >  SRR4302533.5110202/1‑94 (MQ=60)
              TCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATAT                                                                               <  SRR4302533.1249273/94‑1 (MQ=60)
               CGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATG                                                                              <  SRR4302533.3002389/94‑1 (MQ=60)
                GCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGC                                                                             <  SRR4302533.6076252/94‑1 (MQ=60)
                 CGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCC                                                                            <  SRR4302533.5585844/94‑1 (MQ=60)
                 CGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCC                                                                            <  SRR4302533.942476/94‑1 (MQ=60)
                  GCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCG                                                                           <  SRR4302533.611836/94‑1 (MQ=60)
                  GCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCG                                                                           >  SRR4302533.1667272/1‑94 (MQ=60)
                  GCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCG                                                                           <  SRR4302533.5323902/94‑1 (MQ=60)
                     CGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGCAGTGGTCAAGGTCCGCTCCCATATGCCGGAA                                                                        >  SRR4302533.3733754/1‑94 (MQ=60)
                      GAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAA                                                                       >  SRR4302533.1736703/1‑94 (MQ=60)
                      GAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAA                                                                       >  SRR4302533.769823/1‑94 (MQ=60)
                       AAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAAT                                                                      >  SRR4302533.4698875/1‑94 (MQ=60)
                       AAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAAT                                                                      <  SRR4302533.46010/94‑1 (MQ=60)
                         ACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCC                                                                    >  SRR4302533.5807474/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                  <  SRR4302533.4032883/94‑1 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                  >  SRR4302533.6164535/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                  >  SRR4302533.119572/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                  >  SRR4302533.1301171/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                  >  SRR4302533.5676771/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                  >  SRR4302533.4785206/1‑94 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTG                                                                  <  SRR4302533.1084624/94‑1 (MQ=60)
                           CGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTC                                                                  >  SRR4302533.531121/1‑94 (MQ=60)
                             AAGGTCAGGTACTGAAAGCATTGATCGAGCAGCGTCTGGAAGTTGTCTCCGCCGAAGTGGTCAAGTTCCGCTCCTATATGCCGGAAATCCTGCA                                                                >  SRR4302533.6347419/1‑94 (MQ=60)
                             AAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCA                                                                >  SRR4302533.3513691/1‑94 (MQ=60)
                              AGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAA                                                               <  SRR4302533.3751729/94‑1 (MQ=60)
                               GGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAAT                                                              <  SRR4302533.1272003/94‑1 (MQ=60)
                               GGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAAT                                                              <  SRR4302533.3768520/94‑1 (MQ=60)
                                GTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATG                                                             >  SRR4302533.4618521/1‑94 (MQ=60)
                                 TCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGG                                                            >  SRR4302533.5545356/1‑94 (MQ=60)
                                 TCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGG                                                            <  SRR4302533.5769104/94‑1 (MQ=60)
                                   AGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCA                                                          <  SRR4302533.565524/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                         <  SRR4302533.2190543/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                         >  SRR4302533.6106896/1‑94 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                         <  SRR4302533.3073184/94‑1 (MQ=60)
                                    GGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAG                                                         <  SRR4302533.3073182/94‑1 (MQ=60)
                                     GCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGC                                                        >  SRR4302533.2193288/1‑94 (MQ=60)
                                      CACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCG                                                       >  SRR4302533.700881/1‑94 (MQ=60)
                                       ACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGT                                                      >  SRR4302533.3551692/1‑94 (MQ=60)
                                        CTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTG                                                     >  SRR4302533.5337082/1‑94 (MQ=60)
                                        CTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTG                                                     >  SRR4302533.792859/1‑94 (MQ=60)
                                        CTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTG                                                     <  SRR4302533.62248/94‑1 (MQ=60)
                                         TGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGA                                                    <  SRR4302533.4914608/94‑1 (MQ=60)
                                          GAGAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAG                                                   <  SRR4302533.1422917/94‑1 (MQ=60)
                                          GAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAG                                                   <  SRR4302533.5308126/94‑1 (MQ=60)
                                          GAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAG                                                   <  SRR4302533.535809/94‑1 (MQ=60)
                                           AAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGC                                                  <  SRR4302533.186799/94‑1 (MQ=60)
                                              GCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTC                                               >  SRR4302533.714448/1‑94 (MQ=60)
                                               CATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCT                                              <  SRR4302533.3008573/94‑1 (MQ=60)
                                               CATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCT                                              >  SRR4302533.6240856/1‑94 (MQ=60)
                                                ATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTG                                             >  SRR4302533.3741854/1‑94 (MQ=60)
                                                 TTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG                                            >  SRR4302533.4626348/1‑94 (MQ=60)
                                                 TTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG                                            >  SRR4302533.1522439/1‑94 (MQ=60)
                                                  TGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGT                                           >  SRR4302533.641902/1‑94 (MQ=60)
                                                  TGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGT                                           <  SRR4302533.4857578/94‑1 (MQ=60)
                                                   GATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTC                                          <  SRR4302533.938377/94‑1 (MQ=60)
                                                    ATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCG                                         <  SRR4302533.3316713/94‑1 (MQ=60)
                                                    ATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGGCG                                         >  SRR4302533.373508/1‑94 (MQ=60)
                                                     TCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGC                                        >  SRR4302533.5892427/1‑94 (MQ=60)
                                                     TCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGC                                        >  SRR4302533.5922941/1‑94 (MQ=60)
                                                      CGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCG                                       <  SRR4302533.5271675/94‑1 (MQ=60)
                                                         GCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAG                                    <  SRR4302533.2049970/94‑1 (MQ=60)
                                                         GCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTATGGTCGCGAAG                                    >  SRR4302533.3370561/1‑94 (MQ=60)
                                                          CAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGC                                   >  SRR4302533.5341629/1‑94 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                  <  SRR4302533.1001951/94‑1 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                  >  SRR4302533.2618759/1‑94 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                  <  SRR4302533.3610851/94‑1 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                  >  SRR4302533.4829875/1‑94 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                  >  SRR4302533.3461523/1‑94 (MQ=60)
                                                           AGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCT                                  <  SRR4302533.2692039/94‑1 (MQ=60)
                                                            GCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTG                                 >  SRR4302533.752627/1‑94 (MQ=60)
                                                            GCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTG                                 <  SRR4302533.4070791/94‑1 (MQ=60)
                                                             CGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGG                                >  SRR4302533.4564591/1‑94 (MQ=60)
                                                             CGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGG                                >  SRR4302533.4338747/1‑94 (MQ=60)
                                                              GTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA                               >  SRR4302533.3805554/1‑94 (MQ=60)
                                                               gcgGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAA                              <  SRR4302533.1214437/91‑1 (MQ=60)
                                                               TCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAA                              <  SRR4302533.419781/94‑1 (MQ=60)
                                                                  GGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGAT                           <  SRR4302533.5497463/94‑1 (MQ=60)
                                                                     AGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCT                        >  SRR4302533.3466251/1‑94 (MQ=60)
                                                                       GCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCA                      <  SRR4302533.3157353/94‑1 (MQ=60)
                                                                        CGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAG                     <  SRR4302533.2825808/94‑1 (MQ=60)
                                                                        CGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAG                     >  SRR4302533.5964330/1‑94 (MQ=60)
                                                                         GTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGG                    >  SRR4302533.45686/1‑94 (MQ=60)
                                                                         GTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGG                    >  SRR4302533.3924671/1‑94 (MQ=60)
                                                                          TCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGT                   >  SRR4302533.1696927/1‑94 (MQ=60)
                                                                           CACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTG                  >  SRR4302533.4345612/1‑94 (MQ=60)
                                                                           CACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTG                  <  SRR4302533.2866525/94‑1 (MQ=60)
                                                                              CGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAA               <  SRR4302533.2226812/94‑1 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACT             >  SRR4302533.1456997/1‑94 (MQ=60)
                                                                                CCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGAAGCTCAGGTGCAACT             >  SRR4302533.4713368/1‑94 (MQ=60)
                                                                                 CGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTG            <  SRR4302533.2261385/94‑1 (MQ=60)
                                                                                 CGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTG            <  SRR4302533.5537351/94‑1 (MQ=60)
                                                                                  GAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGG           >  SRR4302533.4677885/1‑94 (MQ=60)
                                                                                  GAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGG           >  SRR4302533.2392272/1‑94 (MQ=60)
                                                                                    AGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAA         <  SRR4302533.5269312/94‑1 (MQ=60)
                                                                                      TGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAA       <  SRR4302533.5425459/94‑1 (MQ=60)
                                                                                       GGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAAC      >  SRR4302533.2778339/1‑94 (MQ=60)
                                                                                         TCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAA    >  SRR4302533.2019732/1‑94 (MQ=60)
                                                                                          CAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAAC   <  SRR4302533.5151208/94‑1 (MQ=60)
                                                                                          CAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAAC   <  SRR4302533.5328771/94‑1 (MQ=60)
                                                                                           AAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACC  >  SRR4302533.3816371/1‑94 (MQ=60)
                                                                                             |                                                                                           
GGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACC  >  NC_000913/3814716‑3814900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑