Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,849,668 G→A G248G (GGC→GGT)  ilvB ← acetolactate synthase I large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,849,6680GA99.4% 1221.9 / NA 361G248G (GGC→GGT) ilvBacetolactate synthase I large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (171/187);  total (171/190)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 368 total aligned reads displayed.

CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757
                                                                                          |                                                                                         
caaATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACCACGGATGCGCTTTTGGCAACATAcc                                                                                         >  1:9956365/1‑93 (MQ=255)
 aaaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTCCATCCCCAGCATCCCCAGCGACAACGGATGCGCTTTTGGCAACATGccc                                                                                        <  2:282769/93‑1 (MQ=255)
  aaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCa                                                                                       <  1:9170462/93‑1 (MQ=255)
    tCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgc                                                                                     >  2:10003448/1‑93 (MQ=255)
    tCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgc                                                                                     >  1:8667797/1‑93 (MQ=255)
    tCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgc                                                                                     >  2:2486101/1‑93 (MQ=255)
    tCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgc                                                                                     >  2:38100/1‑93 (MQ=255)
    tCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgc                                                                                     >  2:7042720/1‑93 (MQ=255)
     ccGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgcg                                                                                    <  1:4852978/93‑1 (MQ=255)
       gCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCCGCGcc                                                                                  >  1:6382602/1‑93 (MQ=255)
       gCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGcc                                                                                  >  1:1917970/1‑93 (MQ=255)
       gCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGcc                                                                                  >  2:4368061/1‑93 (MQ=255)
       gCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGcc                                                                                  >  2:4540126/1‑93 (MQ=255)
        cctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGAGAACGGATGCGCTTTTGGCAACATACCCAGCGCCa                                                                                 >  2:8401113/1‑93 (MQ=255)
          tcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAtt                                                                               >  2:1277163/1‑93 (MQ=255)
          tcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAtt                                                                               >  2:3002740/1‑93 (MQ=255)
           cctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTa                                                                              >  1:2794441/1‑93 (MQ=255)
           cctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTa                                                                              <  1:1179876/93‑1 (MQ=255)
           cctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTa                                                                              >  2:199334/1‑93 (MQ=255)
            ctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaa                                                                             >  2:9244066/1‑93 (MQ=255)
             tGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaaa                                                                            <  1:4082/93‑1 (MQ=255)
               cAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGt                                                                          >  2:7898708/1‑93 (MQ=255)
               cAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGt                                                                          >  1:6721616/1‑93 (MQ=255)
               cAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGt                                                                          <  1:5330406/93‑1 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        <  1:5598708/93‑1 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        <  2:9158232/93‑1 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        <  2:5481682/93‑1 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        <  1:238326/93‑1 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        >  1:8303869/1‑93 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       <  2:4287705/93‑1 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       <  2:6676975/93‑1 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      >  1:3855923/1‑93 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      <  2:3181720/93‑1 (MQ=255)
                     ataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggt                                                                    <  2:6814372/93‑1 (MQ=255)
                      taGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggtg                                                                   <  1:354113/93‑1 (MQ=255)
                      taGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggtg                                                                   >  2:3301737/1‑93 (MQ=255)
                       aGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggtgg                                                                  <  1:9695557/93‑1 (MQ=255)
                         ttGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTa                                                                <  1:2947069/93‑1 (MQ=255)
                          tGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAg                                                               >  1:8188488/1‑93 (MQ=255)
                            gTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGc                                                             <  1:3313075/93‑1 (MQ=255)
                             tgctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCa                                                            <  2:5271931/93‑1 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCCTGGAGGTAGGCAg                                                           >  2:5142533/1‑93 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         >  1:3179490/1‑93 (MQ=255)
                                  cgcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgc                                                       <  2:2701696/93‑1 (MQ=255)
                                  cgcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgc                                                       <  2:3587974/93‑1 (MQ=255)
                                    cACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgcgc                                                     >  1:7949308/1‑93 (MQ=255)
                                    cACGCCGTGCATCCCCAGCATACCCAGAGACAACGGATGCGCTTTTGGAAACATACCCAGTGCCATTAAAGTCATGGTGGTAGGCAGTTgcgc                                                     >  2:1073182/1‑93 (MQ=255)
                                     aCGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCt                                                    >  2:2420237/1‑93 (MQ=255)
                                      cGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCtt                                                   <  2:4927116/93‑1 (MQ=255)
                                       gCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCttt                                                  >  1:3947437/1‑93 (MQ=255)
                                        ccGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtc                                                 >  2:8269739/1‑93 (MQ=255)
                                        ccGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtc                                                 >  2:9828175/1‑93 (MQ=255)
                                        ccGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtc                                                 >  1:4317358/1‑93 (MQ=255)
                                        ccGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtc                                                 <  1:6917886/93‑1 (MQ=255)
                                         cGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtct                                                <  1:104304/93‑1 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  2:2235558/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  2:8910597/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               <  1:6055125/93‑1 (MQ=255)
                                           tGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTcc                                              >  2:3976879/1‑93 (MQ=255)
                                           tGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTcc                                              <  1:1694860/93‑1 (MQ=255)
                                             cATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGc                                            <  1:4435597/93‑1 (MQ=255)
                                              aTCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGcc                                           >  1:7862792/1‑93 (MQ=255)
                                               tCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCa                                          <  2:7151874/93‑1 (MQ=255)
                                               tCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCa                                          >  1:1516106/1‑93 (MQ=255)
                                                ccccAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAg                                         >  1:4696362/1‑93 (MQ=255)
                                                  ccAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGtt                                       >  2:4857322/1‑93 (MQ=255)
                                                    aGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCa                                     <  1:3255742/93‑1 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    <  1:8950014/93‑1 (MQ=255)
                                                       aTACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGc                                  <  2:7276054/93‑1 (MQ=255)
                                                         aCCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAc                                >  1:421698/1‑93 (MQ=255)
                                                          cccAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGcccc                               <  1:5779926/93‑1 (MQ=255)
                                                            cAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCg                             <  2:5870830/93‑1 (MQ=255)
                                                             aGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGt                            <  2:8672055/93‑1 (MQ=255)
                                                             aGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGt                            <  2:3719950/93‑1 (MQ=255)
                                                             aGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGt                            <  1:10002679/93‑1 (MQ=255)
                                                              gCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTg                           <  2:4199212/93‑1 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         <  2:4862380/93‑1 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         <  1:9639743/93‑1 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         <  1:7062348/93‑1 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgg                        <  2:4641720/93‑1 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgg                        >  1:3427536/1‑93 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgg                        >  2:7446970/1‑93 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGGCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgg                        <  1:3033697/93‑1 (MQ=255)
                                                                  cAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCggg                       >  1:1747880/1‑93 (MQ=255)
                                                                   aaCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgc                      >  1:8255952/1‑93 (MQ=255)
                                                                   aaCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgc                      >  2:8144335/1‑93 (MQ=255)
                                                                    aCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgcg                     >  1:9187980/1‑93 (MQ=255)
                                                                    aCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgcg                     >  1:4564231/1‑93 (MQ=255)
                                                                      ggATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCa                   >  2:6378609/1‑93 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  >  2:3410168/1‑93 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  >  2:6510706/1‑93 (MQ=255)
                                                                          gcgcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGa               <  1:1574308/93‑1 (MQ=255)
                                                                            gcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATc             <  1:6353084/93‑1 (MQ=255)
                                                                               tttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATcaca          <  1:853313/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         <  2:9529464/93‑1 (MQ=255)
                                                                                 tGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAcc        <  1:5060246/93‑1 (MQ=255)
                                                                                   gCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgc      <  2:4128228/93‑1 (MQ=255)
                                                                                   gCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgc      <  1:7529026/93‑1 (MQ=255)
                                                                                     aaCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgccg    <  1:2603946/93‑1 (MQ=255)
                                                                                       cATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCgccgcc  <  2:1525917/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCA  >  NC_000913/3849575‑3849759
                                                                                             |                                                                                           
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGGTTTTGGCAACATA                                                                                             >  SRR4302540.962847/1‑94 (MQ=60)
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATA                                                                                             <  SRR4302540.7550815/94‑1 (MQ=60)
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATA                                                                                             >  SRR4302540.5516071/1‑94 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                            >  SRR4302540.5546418/1‑94 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                            >  SRR4302540.9708431/1‑94 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                            <  SRR4302540.4619279/94‑1 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                            >  SRR4302540.3291268/1‑94 (MQ=60)
  ACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACCACGGATGCGCTTTTGGCAACATACC                                                                                           >  SRR4302540.10072573/1‑94 (MQ=60)
    AAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCA                                                                                         >  SRR4302540.3998990/1‑94 (MQ=60)
     AATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAG                                                                                        <  SRR4302540.9280469/94‑1 (MQ=60)
     AATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAG                                                                                        <  SRR4302540.8926175/94‑1 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                       >  SRR4302540.5532951/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                       <  SRR4302540.4896375/94‑1 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                       >  SRR4302540.647418/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                       >  SRR4302540.8768481/1‑94 (MQ=60)
       TCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCG                                                                                      >  SRR4302540.6429044/1‑94 (MQ=60)
        CCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGC                                                                                     >  SRR4302540.1572682/1‑94 (MQ=60)
        CCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGC                                                                                     <  SRR4302540.4904991/94‑1 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCCGCGCC                                                                                    >  SRR4302540.6453658/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                    >  SRR4302540.6453657/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                    >  SRR4302540.1938898/1‑94 (MQ=60)
          GCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCA                                                                                   >  SRR4302540.8552794/1‑94 (MQ=60)
          GCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCA                                                                                   >  SRR4302540.4649941/1‑94 (MQ=60)
            CTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATT                                                                                 >  SRR4302540.10159799/1‑94 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                >  SRR4302540.2824613/1‑94 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                >  SRR4302540.5795816/1‑94 (MQ=60)
              CCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAA                                                                               <  SRR4302540.1192064/94‑1 (MQ=60)
              CCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAA                                                                               >  SRR4302540.6384571/1‑94 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAA                                                                              >  SRR4302540.1741064/1‑94 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                             <  SRR4302540.4127/94‑1 (MQ=60)
                 GCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGT                                                                            >  SRR4302540.6795801/1‑94 (MQ=60)
                 GCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGT                                                                            >  SRR4302540.6077558/1‑94 (MQ=60)
                  CAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTC                                                                           <  SRR4302540.5388802/94‑1 (MQ=60)
                  CAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTC                                                                           >  SRR4302540.5935722/1‑94 (MQ=60)
                   AAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCA                                                                          <  SRR4302540.7704361/94‑1 (MQ=60)
                   AAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCA                                                                          <  SRR4302540.7116000/94‑1 (MQ=60)
                   AAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCA                                                                          >  SRR4302540.8400294/1‑94 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                         <  SRR4302540.4045117/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                         <  SRR4302540.1041718/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                         <  SRR4302540.240641/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                         <  SRR4302540.5660303/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                         <  SRR4302540.3679158/94‑1 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                        >  SRR4302540.3897845/1‑94 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                        <  SRR4302540.8390340/94‑1 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                       <  SRR4302540.2992961/94‑1 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACACACCCAGCGCCATTAAAGTCATGG                                                                       <  SRR4302540.2992960/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                     >  SRR4302540.8975083/1‑94 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                     >  SRR4302540.9291230/1‑94 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                     >  SRR4302540.531463/1‑94 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                    <  SRR4302540.8748585/94‑1 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                    <  SRR4302540.357744/94‑1 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                    <  SRR4302540.5403783/94‑1 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                    <  SRR4302540.3336442/94‑1 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                    <  SRR4302540.508582/94‑1 (MQ=60)
                          AGTTGGTGCTGCGCGCGCGGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGT                                                                   <  SRR4302540.2025026/94‑1 (MQ=60)
                          AGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGT                                                                   <  SRR4302540.9809570/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 >  SRR4302540.3085520/1‑94 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 <  SRR4302540.4608415/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 <  SRR4302540.1595291/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 <  SRR4302540.8383084/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 >  SRR4302540.8283624/1‑94 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 <  SRR4302540.824690/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 <  SRR4302540.1866359/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 <  SRR4302540.2279269/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                 <  SRR4302540.2979497/94‑1 (MQ=60)
                             TGGTGCTGCGCACGCCGTGCATCCCCGGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTCGG                                                                >  SRR4302540.4009326/1‑94 (MQ=60)
                             TGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGG                                                                <  SRR4302540.5323744/94‑1 (MQ=60)
                             TGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGG                                                                >  SRR4302540.6948334/1‑94 (MQ=60)
                              GGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGC                                                               <  SRR4302540.3332909/94‑1 (MQ=60)
                               GTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCA                                                              <  SRR4302540.3349378/94‑1 (MQ=60)
                                  CTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTT                                                           >  SRR4302540.6836687/1‑94 (MQ=60)
                                  CTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTT                                                           >  SRR4302540.4783570/1‑94 (MQ=60)
                                  CTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTT                                                           <  SRR4302540.1153832/94‑1 (MQ=60)
                                  CTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTT                                                           >  SRR4302540.3214037/1‑94 (MQ=60)
                                   TGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTG                                                          >  SRR4302540.5085645/1‑94 (MQ=60)
                                   TGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTG                                                          <  SRR4302540.4206485/94‑1 (MQ=60)
                                    GCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGC                                                         <  SRR4302540.3937334/94‑1 (MQ=60)
                                     CGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCG                                                        >  SRR4302540.1666945/1‑94 (MQ=60)
                                     CGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCG                                                        <  SRR4302540.108890/94‑1 (MQ=60)
                                     CGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCG                                                        <  SRR4302540.4235799/94‑1 (MQ=60)
                                      GCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGC                                                       >  SRR4302540.8041977/1‑94 (MQ=60)
                                      GCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGC                                                       >  SRR4302540.9801278/1‑94 (MQ=60)
                                      GCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGC                                                       >  SRR4302540.10058129/1‑94 (MQ=60)
                                       CACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCT                                                      >  SRR4302540.1803479/1‑94 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCTACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGTTGTTACGCAATTGCGCTTT                                                    >  SRR4302540.7348161/1‑94 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTT                                                    >  SRR4302540.3990055/1‑94 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCAACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTCAAGTCATGGTGGTAGGCAGTTGCGCTTT                                                    >  SRR4302540.5794390/1‑94 (MQ=60)
                                          GCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTC                                                   <  SRR4302540.10090216/94‑1 (MQ=60)
                                          GCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTC                                                   >  SRR4302540.4364359/1‑94 (MQ=60)
                                          GCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTC                                                   >  SRR4302540.9713700/1‑94 (MQ=60)
                                          CCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTC                                                   <  SRR4302540.9167477/94‑1 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                  <  SRR4302540.6994865/94‑1 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                 >  SRR4302540.1605143/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                 <  SRR4302540.105415/94‑1 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                 >  SRR4302540.7811364/1‑94 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                <  SRR4302540.6122420/94‑1 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                <  SRR4302540.7994708/94‑1 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                >  SRR4302540.1582749/1‑94 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                <  SRR4302540.1425203/94‑1 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                <  SRR4302540.4530833/94‑1 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                >  SRR4302540.2027665/1‑94 (MQ=60)
                                              TGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCG                                               <  SRR4302540.1713232/94‑1 (MQ=60)
                                              TGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCC                                               >  SRR4302540.833632/1‑94 (MQ=60)
                                               GCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGC                                              <  SRR4302540.9260222/94‑1 (MQ=60)
                                               GCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGC                                              <  SRR4302540.7597883/94‑1 (MQ=60)
                                                CATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCC                                             >  SRR4302540.7954655/1‑94 (MQ=60)
                                                CATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCC                                             <  SRR4302540.4483659/94‑1 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                            >  SRR4302540.3927584/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                            >  SRR4302540.1532875/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                            <  SRR4302540.3510170/94‑1 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                            >  SRR4302540.9797790/1‑94 (MQ=60)
                                                  TCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAG                                           <  SRR4302540.4349652/94‑1 (MQ=60)
                                                  TCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAG                                           >  SRR4302540.4746563/1‑94 (MQ=60)
                                                  TCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAG                                           <  SRR4302540.2429359/94‑1 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGT                                          >  SRR4302540.4160044/1‑94 (MQ=60)
                                                    CCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTT                                         <  SRR4302540.8343315/94‑1 (MQ=60)
                                                    CCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTT                                         >  SRR4302540.3249387/1‑94 (MQ=60)
                                                    CCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTT                                         >  SRR4302540.8152069/1‑94 (MQ=60)
                                                     CCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTC                                        <  SRR4302540.7993552/94‑1 (MQ=60)
                                                     CCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTC                                        <  SRR4302540.2782164/94‑1 (MQ=60)
                                                     CCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTC                                        <  SRR4302540.4471/94‑1 (MQ=60)
                                                      CAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCA                                       <  SRR4302540.1312206/94‑1 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                      <  SRR4302540.3291228/94‑1 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                      >  SRR4302540.8236537/1‑94 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                      <  SRR4302540.7990850/94‑1 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                      >  SRR4302540.9780772/1‑94 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCCGGTGCGTTTTCTCCGCCAGTTCCCG                                     >  SRR4302540.346174/1‑94 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACG                                     >  SRR4302540.346175/1‑94 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACG                                     <  SRR4302540.8719126/94‑1 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACG                                     <  SRR4302540.9055465/94‑1 (MQ=60)
                                                         CATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGC                                    >  SRR4302540.6741021/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCACCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTGTCTCCGCCAGTTCACGCC                                   >  SRR4302540.3518031/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCC                                   >  SRR4302540.514436/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                   >  SRR4302540.263237/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                   >  SRR4302540.3518032/1‑94 (MQ=60)
                                                           TACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAC                                  >  SRR4302540.426135/1‑94 (MQ=60)
                                                           TACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAC                                  <  SRR4302540.568179/94‑1 (MQ=60)
                                                           TACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAC                                  <  SRR4302540.2996750/94‑1 (MQ=60)
                                                            ACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACC                                 <  SRR4302540.5896/94‑1 (MQ=60)
                                                            ACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACC                                 <  SRR4302540.9227309/94‑1 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCCCCC                                <  SRR4302540.5843776/94‑1 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                <  SRR4302540.4415523/94‑1 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                >  SRR4302540.6418890/1‑94 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                <  SRR4302540.4589263/94‑1 (MQ=60)
                                                               CAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGT                              <  SRR4302540.469755/94‑1 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                             <  SRR4302540.10119196/94‑1 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                             <  SRR4302540.7110490/94‑1 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGGGCG                           >  SRR4302540.174663/1‑94 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                          <  SRR4302540.7140898/94‑1 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                          >  SRR4302540.3464786/1‑94 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                          <  SRR4302540.9753303/94‑1 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                          >  SRR4302540.448142/1‑94 (MQ=60)
                                                                    ACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGG                         >  SRR4302540.1766807/1‑94 (MQ=60)
                                                                    ACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGG                         >  SRR4302540.1766806/1‑94 (MQ=60)
                                                                    ACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGGCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGG                         <  SRR4302540.3066993/94‑1 (MQ=60)
                                                                     CAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGC                        <  SRR4302540.7544118/94‑1 (MQ=60)
                                                                     CAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGC                        >  SRR4302540.8351902/1‑94 (MQ=60)
                                                                     CAACCGATGCTCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGC                        <  SRR4302540.8871482/94‑1 (MQ=60)
                                                                      AACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCG                       >  SRR4302540.9298166/1‑94 (MQ=60)
                                                                      AACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCG                       >  SRR4302540.4613525/1‑94 (MQ=60)
                                                                      AACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCG                       >  SRR4302540.1482011/1‑94 (MQ=60)
                                                                        CGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCA                     <  SRR4302540.9577895/94‑1 (MQ=60)
                                                                          GATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATT                   <  SRR4302540.10001409/94‑1 (MQ=60)
                                                                          GATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATT                   >  SRR4302540.3319952/1‑94 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                  <  SRR4302540.6620847/94‑1 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                  >  SRR4302540.10094101/1‑94 (MQ=60)
                                                                             GCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGAT                <  SRR4302540.1591544/94‑1 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC               >  SRR4302540.1153174/1‑94 (MQ=60)
                                                                               GCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCA              >  SRR4302540.927784/1‑94 (MQ=60)
                                                                               GCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCA              <  SRR4302540.6423835/94‑1 (MQ=60)
                                                                                CTTTTGGCAACATACCGAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC             >  SRR4302540.9910451/1‑94 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC             <  SRR4302540.8382346/94‑1 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC             <  SRR4302540.8615299/94‑1 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC             >  SRR4302540.7125215/1‑94 (MQ=60)
                                                                                 TTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACA            <  SRR4302540.8539480/94‑1 (MQ=60)
                                                                                 TTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACA            <  SRR4302540.4734203/94‑1 (MQ=60)
                                                                                  TTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAC           >  SRR4302540.9730598/1‑94 (MQ=60)
                                                                                  TTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAC           <  SRR4302540.2566748/94‑1 (MQ=60)
                                                                                  TTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAC           <  SRR4302540.2566747/94‑1 (MQ=60)
                                                                                  TTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAC           <  SRR4302540.862378/94‑1 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC          <  SRR4302540.29040/94‑1 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC          <  SRR4302540.3174649/94‑1 (MQ=60)
                                                                                    TGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCG         <  SRR4302540.5114957/94‑1 (MQ=60)
                                                                                      GCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCC       <  SRR4302540.3767439/94‑1 (MQ=60)
                                                                                      GCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCC       <  SRR4302540.7613299/94‑1 (MQ=60)
                                                                                      GCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCC       <  SRR4302540.435933/94‑1 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG      <  SRR4302540.3276038/94‑1 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG      <  SRR4302540.3276037/94‑1 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG      <  SRR4302540.5001072/94‑1 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG      <  SRR4302540.2864582/94‑1 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG      <  SRR4302540.2864326/94‑1 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG      >  SRR4302540.6436259/1‑94 (MQ=60)
                                                                                        AACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGC     <  SRR4302540.2632036/94‑1 (MQ=60)
                                                                                        AACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGC     >  SRR4302540.3802714/1‑94 (MQ=60)
                                                                                          CATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCC   >  SRR4302540.2162989/1‑94 (MQ=60)
                                                                                           ATTCCCAGCGCCATTAAAGTCATGGTCGCAGTCAGTTGCGCGTTCCACGCCAGTTCACGCATACGTGCGGGCGCATTGATCACACCGCCGCCCA  <  SRR4302540.5213732/94‑1 (MQ=57)
                                                                                           ATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCA  >  SRR4302540.438010/1‑94 (MQ=60)
                                                                                             |                                                                                           
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCA  >  NC_000913/3849575‑3849759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑