Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,058	G→A	G118G (GGC→GGT)	ilvB ←	acetolactate synthase I large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,058	0	G	A	99.1%	1226.1 / ‑3.3	344	G118G (GGC→GGT)	ilvB	acetolactate synthase I large subunit
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (199/142); total (201/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

Only 100 of 363 total aligned reads displayed.

CGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGT  >  NC_000913/3849968‑3850147

 gggAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCg                                                                                        >  1:245160/1‑93 (MQ=255)

    aGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGatc                                                                                     >  1:553331/1‑93 (MQ=255)

    aGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGatc                                                                                     <  2:8416221/93‑1 (MQ=255)

    aGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGatc                                                                                     <  1:3061359/93‑1 (MQ=255)

    aGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGatc                                                                                     <  1:8238754/93‑1 (MQ=255)

     gTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGatca                                                                                    >  2:7781698/1‑93 (MQ=255)

      ttcttcGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGatcat                                                                                   <  1:1055582/93‑1 (MQ=255)

       tcttcGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGatcatc                                                                                  >  1:6103853/1‑93 (MQ=255)

        cttcGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCg                                                                                 <  2:2814356/93‑1 (MQ=255)

         ttcGATTTGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGGTTCCACTTCATGGAAGGCGTCGGTACCGATCATCGa                                                                                >  1:2355667/1‑93 (MQ=255)

          tcGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAg                                                                               >  2:1211210/1‑93 (MQ=255)

          tcGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAg                                                                               <  1:2110918/93‑1 (MQ=255)

           cGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAgg                                                                              >  1:285278/1‑93 (MQ=255)

           cGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAgg                                                                              <  2:6266879/93‑1 (MQ=255)

           cGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAgg                                                                              <  1:7404851/93‑1 (MQ=255)

            gATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAggg                                                                             >  1:899942/1‑92 (MQ=255)

            gATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGc                                                                             >  2:2256554/1‑93 (MQ=255)

            gATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGc                                                                             >  1:6002474/1‑93 (MQ=255)

            gATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGc                                                                             <  1:9856187/93‑1 (MQ=255)

            gATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGc                                                                             <  1:1685873/93‑1 (MQ=255)

            gATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGc                                                                             >  1:2698344/1‑93 (MQ=255)

             atatGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCg                                                                            >  2:4986005/1‑93 (MQ=255)

             atatGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCg                                                                            >  1:9720616/1‑93 (MQ=255)

             atatGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCg                                                                            >  1:9585667/1‑93 (MQ=255)

              tatGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCgg                                                                           >  2:556033/1‑93 (MQ=255)

              tatGTCTGACCAGATAGATGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGcggcgg                                                                           >  1:4477559/1‑93 (MQ=255)

                tGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGa                                                                         >  2:4678978/1‑93 (MQ=255)

                tGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGa                                                                         >  1:1790056/1‑93 (MQ=255)

                  tCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAAc                                                                       <  2:2610392/93‑1 (MQ=255)

                  tCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATAGAGGCGGGAAc                                                                       <  1:4826610/93‑1 (MQ=255)

                   cTGACCAGATAGGTGTGTTGGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAAcc                                                                      <  1:7964704/93‑1 (MQ=255)

                    tGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCt                                                                     >  2:1102743/1‑93 (MQ=255)

                    tGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCt                                                                     <  2:4094373/93‑1 (MQ=255)

                     gACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTg                                                                    >  1:3167407/1‑93 (MQ=255)

                        cAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGAcc                                                                 >  2:4630300/1‑93 (MQ=255)

                        cAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGAcc                                                                 >  2:9232495/1‑93 (MQ=255)

                        cAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCAGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGAcc                                                                 <  2:2097246/93‑1 (MQ=255)

                         aGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCa                                                                <  2:4523483/93‑1 (MQ=255)

                          gATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGGACCTGACCAg                                                               >  2:7166246/1‑93 (MQ=255)

                           aTAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCaag                                                              >  2:7403753/1‑91 (MQ=255)

                           aTAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGt                                                              <  1:7289068/93‑1 (MQ=255)

                            tAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTg                                                             >  2:868458/1‑93 (MQ=255)

                              gTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAt                                                           >  1:8547075/1‑93 (MQ=255)

                              gTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAt                                                           <  2:4344459/93‑1 (MQ=255)

                              gTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAt                                                           >  1:5332909/1‑93 (MQ=255)

                                tgtgTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGc                                                         >  2:645033/1‑93 (MQ=255)

                                tgtgTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGc                                                         >  1:8849580/1‑93 (MQ=255)

                                tgtgTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGc                                                         <  1:4102712/93‑1 (MQ=255)

                                 gtgtTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCa                                                        >  1:7908676/1‑93 (MQ=255)

                                   gtTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCaaa                                                      >  1:4017609/1‑93 (MQ=255)

                                     ttGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATc                                                    <  1:6537901/93‑1 (MQ=255)

                                        gTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGc                                                 <  2:291145/93‑1 (MQ=255)

                                        gTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGc                                                 <  1:7056586/93‑1 (MQ=255)

                                         tGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCg                                                <  2:3911559/93‑1 (MQ=255)

                                         tGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCg                                                >  1:1170223/1‑93 (MQ=255)

                                          gATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCgg                                               >  1:1520166/1‑93 (MQ=255)

                                            tGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGa                                             <  2:9707432/93‑1 (MQ=255)

                                            tGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGa                                             <  1:9365697/93‑1 (MQ=255)

                                               ggATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGGTgg                                          >  2:1647090/1‑93 (MQ=255)

                                                 aTAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAg                                        >  2:5876160/1‑93 (MQ=255)

                                                  tAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGt                                       >  1:6362184/1‑93 (MQ=255)

                                                    gagaTGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTcc                                     >  2:5080594/1‑93 (MQ=255)

                                                     agaTGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCa                                    >  2:3572608/1‑93 (MQ=255)

                                                       aTGCCGTAGGTGTCCACTTCCTGGAATGCTTCTGGACCGGTGATCGCGGCGGGAACCTGACCAGGGAGTCAAATCAGCGGGAGGGAGTCTAGc                                  >  2:664669/1‑93 (MQ=255)

                                                       aTGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGc                                  <  1:4298880/93‑1 (MQ=255)

                                                          ccGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCcgc                               <  1:3920703/93‑1 (MQ=255)

                                                          ccGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCcgc                               >  1:6710549/1‑93 (MQ=255)

                                                           cGTAGGCATCCACTTCCTGGAAGGAGTCGGTACCGCTCATCGAGGCGGGTACATGACCACTGGTGCAAATAACCGAGATTGAGGCCAGAcgcg                              >  2:4842594/1‑93 (MQ=255)

                                                            gTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCgcgc                             >  1:7776951/1‑93 (MQ=255)

                                                             tAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCa                            >  2:9846255/1‑93 (MQ=255)

                                                             tAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCa                            <  2:9534744/93‑1 (MQ=255)

                                                             tAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCa                            <  2:9408890/93‑1 (MQ=255)

                                                             tAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCa                            >  1:4710036/1‑93 (MQ=255)

                                                             tAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCa                            >  2:3242918/1‑93 (MQ=255)

                                                              aGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCAt                           >  2:3965793/1‑93 (MQ=255)

                                                                gtgtCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGGGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCg                         >  1:1615193/1‑93 (MQ=255)

                                                                   tCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCa                      >  1:10014161/1‑93 (MQ=255)

                                                                   tCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCa                      >  2:7686153/1‑93 (MQ=255)

                                                                   tCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCa                      >  2:9041777/1‑93 (MQ=255)

                                                                    ccACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCaa                     <  1:7614620/93‑1 (MQ=255)

                                                                     cACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAAt                    >  1:9003087/1‑93 (MQ=255)

                                                                      aCTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATg                   <  2:8006809/93‑1 (MQ=255)

                                                                        ttCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGc                 <  2:6788629/93‑1 (MQ=255)

                                                                         tCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCg                >  2:8347444/1‑93 (MQ=255)

                                                                          ccTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCgg               >  1:3627741/1‑93 (MQ=255)

                                                                           cTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGt              <  2:8551757/93‑1 (MQ=255)

                                                                           cTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGt              <  1:493232/93‑1 (MQ=255)

                                                                           cTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGGGATGCAAATCAGCGGGATGGAGGCCAGCCGCGCATCGGCAAGGGCGGt              >  2:4465826/1‑93 (MQ=255)

                                                                            tGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTc             >  1:5845491/1‑93 (MQ=255)

                                                                              gAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCAc           >  2:5289296/1‑93 (MQ=255)

                                                                               aaGCCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCAcc          <  2:6879915/93‑1 (MQ=255)

                                                                                aGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCa         >  2:3750510/1‑93 (MQ=255)

                                                                                aGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCa         >  2:238599/1‑93 (MQ=255)

                                                                                  gCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAgg       <  2:9115066/93‑1 (MQ=255)

                                                                                   cGTTGGTACCGATCATCGAGGCGGTAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGt      <  1:3309787/93‑1 (MQ=255)

                                                                                     tCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTGCAGCCGCGCATCGGCAATGGCGGTCAACAGGATa    >  1:294330/1‑93 (MQ=255)

                                                                                     tCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCCATGGCGGTCACCAGGTTa    >  1:7573399/1‑93 (MQ=255)

                                                                                     tCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTa    >  2:1966711/1‑93 (MQ=255)

                                                                                       ggTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGt  <  1:1356019/93‑1 (MQ=255)

                                                                                       ggTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGt  <  2:6137626/93‑1 (MQ=255)

CGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGT  >  NC_000913/3849968‑3850147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 207 total aligned reads displayed.

CTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCA  >  NC_000913/3849965‑3850151

CTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGACGGCGTCGGTA                                                                                               >  SRR4302540.4543542/1‑94 (MQ=60)

CTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTA                                                                                               <  SRR4302540.5766353/94‑1 (MQ=60)

CTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTA                                                                                               >  SRR4302540.4467427/1‑94 (MQ=60)

CTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTA                                                                                               <  SRR4302540.1126409/94‑1 (MQ=60)

 TGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTAC                                                                                              <  SRR4302540.7345161/94‑1 (MQ=60)

 TGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTAC                                                                                              <  SRR4302540.3820426/94‑1 (MQ=60)

 TGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTAC                                                                                              >  SRR4302540.1733665/1‑94 (MQ=60)

  GCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACC                                                                                             <  SRR4302540.3296495/94‑1 (MQ=60)

  GCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACC                                                                                             <  SRR4302540.253244/94‑1 (MQ=60)

   CGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCG                                                                                            >  SRR4302540.7301067/1‑94 (MQ=60)

   CGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCG                                                                                            >  SRR4302540.247530/1‑94 (MQ=60)

    GGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGA                                                                                           >  SRR4302540.3793625/1‑94 (MQ=60)

    GGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGA                                                                                           >  SRR4302540.7669676/1‑94 (MQ=60)

    GGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGA                                                                                           >  SRR4302540.1570578/1‑94 (MQ=60)

    GGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGA                                                                                           >  SRR4302540.10088987/1‑94 (MQ=60)

     GGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGAT                                                                                          >  SRR4302540.353640/1‑94 (MQ=60)

      GAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATC                                                                                         >  SRR4302540.559364/1‑94 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCA                                                                                        <  SRR4302540.2942961/94‑1 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCA                                                                                        <  SRR4302540.7279516/94‑1 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCA                                                                                        <  SRR4302540.3094947/94‑1 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCA                                                                                        >  SRR4302540.7131876/1‑94 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCA                                                                                        <  SRR4302540.8334505/94‑1 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCA                                                                                        >  SRR4302540.3297026/1‑94 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCA                                                                                        <  SRR4302540.9160832/94‑1 (MQ=60)

       AGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGAGTCGGTACCGATac                                                                                        >  SRR4302540.7131877/1‑92 (MQ=60)

        GTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCAT                                                                                       >  SRR4302540.1427464/1‑94 (MQ=60)

         TTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATC                                                                                      <  SRR4302540.1066323/94‑1 (MQ=60)

         TTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATC                                                                                      >  SRR4302540.6171823/1‑94 (MQ=60)

         TTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATC                                                                                      >  SRR4302540.6646722/1‑94 (MQ=60)

         TTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATC                                                                                      >  SRR4302540.5792872/1‑94 (MQ=60)

          TCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCG                                                                                     <  SRR4302540.6432036/94‑1 (MQ=60)

           CTTCGATTTGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGGTTCCACTTCATGGAAGGCGTCGGTACCGATCATCGA                                                                                    >  SRR4302540.2381039/1‑94 (MQ=60)

           CTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGA                                                                                    >  SRR4302540.1723622/1‑94 (MQ=60)

            TTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAG                                                                                   >  SRR4302540.9847806/1‑94 (MQ=60)

             TCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGG                                                                                  <  SRR4302540.2133572/94‑1 (MQ=60)

             TCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGG                                                                                  >  SRR4302540.288088/1‑94 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGG                                                                                 >  SRR4302540.3152589/1‑94 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGG                                                                                 >  SRR4302540.909506/1‑94 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGG                                                                                 >  SRR4302540.1030784/1‑94 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGC                                                                                 >  SRR4302540.2727470/1‑94 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGC                                                                                 >  SRR4302540.6069003/1‑94 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGC                                                                                 <  SRR4302540.7487348/94‑1 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGC                                                                                 <  SRR4302540.2446319/94‑1 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGC                                                                                 >  SRR4302540.3156626/1‑94 (MQ=60)

              CGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGC                                                                                 <  SRR4302540.5596950/94‑1 (MQ=60)

               GATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCG                                                                                >  SRR4302540.9834810/1‑94 (MQ=60)

               GATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCG                                                                                >  SRR4302540.9698832/1‑94 (MQ=60)

               GATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCG                                                                                <  SRR4302540.1704164/94‑1 (MQ=60)

               GATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCG                                                                                <  SRR4302540.6435645/94‑1 (MQ=60)

               GATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCG                                                                                >  SRR4302540.906933/1‑94 (MQ=60)

                ATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGG                                                                               >  SRR4302540.3015313/1‑94 (MQ=60)

                ATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGG                                                                               >  SRR4302540.2126277/1‑94 (MQ=60)

                ATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGG                                                                               >  SRR4302540.9274755/1‑94 (MQ=60)

                ATATGTCTGACCAGATAGATGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGCGGCGG                                                                               >  SRR4302540.4526128/1‑94 (MQ=60)

                  ATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGA                                                                             >  SRR4302540.9569228/1‑94 (MQ=60)

                  ATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGA                                                                             >  SRR4302540.2638572/1‑94 (MQ=60)

                  ATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGA                                                                             >  SRR4302540.1809511/1‑94 (MQ=60)

                   TGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATCCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAA                                                                            <  SRR4302540.2127569/94‑1 (MQ=60)

                    GTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAAC                                                                           <  SRR4302540.788440/94‑1 (MQ=60)

                     TCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATAGAGGCGGGAACC                                                                          <  SRR4302540.4878296/94‑1 (MQ=60)

                      CTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCT                                                                         <  SRR4302540.8057525/94‑1 (MQ=60)

                      CTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCT                                                                         >  SRR4302540.165855/1‑94 (MQ=60)

                      CTGACCAGATAGGTGTGTTGGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCT                                                                         <  SRR4302540.8057526/94‑1 (MQ=60)

                       TGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTG                                                                        <  SRR4302540.4525121/94‑1 (MQ=60)

                       TGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTG                                                                        >  SRR4302540.3201843/1‑94 (MQ=60)

                        GACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGA                                                                       >  SRR4302540.8269423/1‑94 (MQ=60)

                        GACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGA                                                                       >  SRR4302540.5140763/1‑94 (MQ=60)

                        GACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGA                                                                       >  SRR4302540.6310387/1‑94 (MQ=60)

                          CCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACC                                                                     >  SRR4302540.658473/1‑94 (MQ=60)

                          CCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACC                                                                     <  SRR4302540.613507/94‑1 (MQ=60)

                           CAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATGATCGAGGCGGGAcactgacga                                                                    >  SRR4302540.8469510/1‑85 (MQ=60)

                           CAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTACTGGAAGGCGTCGGTACCGATCATAGAGGCGGGGACCTGACCA                                                                    >  SRR4302540.3866660/1‑94 (MQ=60)

                            AGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAG                                                                   <  SRR4302540.7420126/94‑1 (MQ=60)

                            AGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAG                                                                   >  SRR4302540.3267248/1‑94 (MQ=60)

                            AGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAG                                                                   >  SRR4302540.1597253/1‑94 (MQ=60)

                             GATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGG                                                                  >  SRR4302540.43141/1‑94 (MQ=60)

                             GATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAAACTGACCAGT                                                                  >  SRR4302540.9014261/1‑94 (MQ=60)

                              ATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTG                                                                 >  SRR4302540.7540039/1‑94 (MQ=60)

                              ATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTG                                                                 <  SRR4302540.7370410/94‑1 (MQ=60)

                              ATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTG                                                                 >  SRR4302540.79961/1‑94 (MQ=60)

                                AGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAT                                                               >  SRR4302540.5391348/1‑94 (MQ=60)

                                AGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAT                                                               >  SRR4302540.654055/1‑94 (MQ=60)

                                AGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAT                                                               >  SRR4302540.8646367/1‑94 (MQ=60)

                                AGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAT                                                               <  SRR4302540.9821338/94‑1 (MQ=60)

                                AGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGAT                                                               >  SRR4302540.3560654/1‑94 (MQ=60)

                                 GTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATG                                                              >  SRR4302540.7768412/1‑94 (MQ=60)

                                 GTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATG                                                              <  SRR4302540.743174/94‑1 (MQ=60)

                                 GTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATG                                                              >  SRR4302540.8061905/1‑94 (MQ=60)

                                  TTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGC                                                             >  SRR4302540.3995363/1‑94 (MQ=60)

                                  TTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGC                                                             >  SRR4302540.8951962/1‑94 (MQ=60)

                                   TGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCA                                                            <  SRR4302540.4146973/94‑1 (MQ=60)

                                   TGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCA                                                            >  SRR4302540.8000897/1‑94 (MQ=60)

                                   TGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCA                                                            >  SRR4302540.1095844/1‑94 (MQ=60)

                                     TGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAA                                                          >  SRR4302540.4060887/1‑94 (MQ=60)

                                     TGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAA                                                          >  SRR4302540.4173870/1‑94 (MQ=60)

                                      GTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAAT                                                         >  SRR4302540.6754008/1‑94 (MQ=60)

                                      GTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAAT                                                         <  SRR4302540.133548/94‑1 (MQ=60)

                                       TTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATC                                                        >  SRR4302540.3074057/1‑94 (MQ=60)

                                       TTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATC                                                        <  SRR4302540.10073688/94‑1 (MQ=60)

                                       TTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATC                                                        <  SRR4302540.4140753/94‑1 (MQ=60)

                                       TTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAAAC                                                        <  SRR4302540.9732994/94‑1 (MQ=60)

                                        TTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCA                                                       <  SRR4302540.6610456/94‑1 (MQ=60)

                                        TTGGTGATGGGGATAGAGATGCCGTAGGAGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCA                                                       <  SRR4302540.2753392/94‑1 (MQ=60)

                                         TGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAG                                                      <  SRR4302540.6068071/94‑1 (MQ=60)

                                          GGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGC                                                     <  SRR4302540.2336425/94‑1 (MQ=60)

                                          GGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGC                                                     <  SRR4302540.2436061/94‑1 (MQ=60)

                                           GTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCG                                                    >  SRR4302540.1182341/1‑94 (MQ=60)

                                           GTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCG                                                    <  SRR4302540.7135060/94‑1 (MQ=60)

                                            TGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGG                                                   >  SRR4302540.1536993/1‑94 (MQ=60)

                                            TGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGAAAATCAGCGG                                                   >  SRR4302540.1543790/1‑94 (MQ=60)

                                             GATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGG                                                  <  SRR4302540.2893780/94‑1 (MQ=60)

                                               TGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGAT                                                <  SRR4302540.9477215/94‑1 (MQ=60)

                                               TGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGAT                                                <  SRR4302540.6210838/94‑1 (MQ=60)

                                               TGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGAT                                                <  SRR4302540.7911779/94‑1 (MQ=60)

                                               TGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGAT                                                <  SRR4302540.7911780/94‑1 (MQ=60)

                                                 GGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGG                                              <  SRR4302540.2788144/94‑1 (MQ=60)

                                                   GATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAG                                            >  SRR4302540.9935065/1‑94 (MQ=60)

                                                    ATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGT                                           >  SRR4302540.6433030/1‑94 (MQ=60)

                                                    ATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGT                                           >  SRR4302540.5343971/1‑94 (MQ=60)

                                                    ATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGT                                           >  SRR4302540.5406893/1‑94 (MQ=60)

                                                    ATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGT                                           >  SRR4302540.4946481/1‑94 (MQ=60)

                                                     TAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTC                                          <  SRR4302540.5493857/94‑1 (MQ=60)

                                                     TAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTC                                          >  SRR4302540.1717405/1‑94 (MQ=60)

                                                      AGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCC                                         >  SRR4302540.7720458/1‑94 (MQ=60)

                                                      AGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCC                                         >  SRR4302540.4132525/1‑94 (MQ=60)

                                                       GACATGACGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCA                                        <  SRR4302540.4855853/94‑1 (MQ=60)

                                                        AGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCTGGAACCTGACCAGCGATGCAAATCAGCGGGATGGAGTCCAG                                       >  SRR4302540.9056279/1‑94 (MQ=60)

                                                          ATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCC                                     <  SRR4302540.4345671/94‑1 (MQ=60)

                                                          ATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCC                                     >  SRR4302540.1081013/1‑94 (MQ=60)

                                                          ATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCC                                     <  SRR4302540.4409359/94‑1 (MQ=60)

                                                          ATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTACAGCG                                     >  SRR4302540.7679830/1‑94 (MQ=60)

                                                          ATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGGCCAGCC                                     >  SRR4302540.4109944/1‑94 (MQ=60)

                                                           TGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCG                                    >  SRR4302540.8756878/1‑94 (MQ=60)

                                                            GCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGC                                   <  SRR4302540.1244972/94‑1 (MQ=60)

                                                            GCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGC                                   <  SRR4302540.7556985/94‑1 (MQ=60)

                                                            GCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGC                                   >  SRR4302540.6784598/1‑94 (MQ=60)

                                                            GCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGC                                   >  SRR4302540.459198/1‑94 (MQ=60)

                                                             CCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCG                                  <  SRR4302540.3963137/94‑1 (MQ=60)

                                                             CCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCG                                  >  SRR4302540.6905569/1‑94 (MQ=60)

                                                              CGTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGC                                 >  SRR4302540.7867865/1‑94 (MQ=60)

                                                               GTAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCA                                >  SRR4302540.4760375/1‑94 (MQ=60)

                                                                TAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCAT                               <  SRR4302540.3974890/94‑1 (MQ=60)

                                                                TAGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCAT                               >  SRR4302540.4883864/1‑94 (MQ=60)

                                                                 AGGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATC                              <  SRR4302540.4849623/94‑1 (MQ=60)

                                                                  GGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCG                             >  SRR4302540.1632914/1‑94 (MQ=60)

                                                                  GGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGGGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCG                             >  SRR4302540.1632913/1‑94 (MQ=60)

                                                                  GGTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGGGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCG                             >  SRR4302540.5518383/1‑94 (MQ=60)

                                                                   GTGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGG                            >  SRR4302540.9648519/1‑94 (MQ=60)

                                                                    TGTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGC                           <  SRR4302540.9108547/94‑1 (MQ=60)

                                                                     GTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCA                          >  SRR4302540.4819791/1‑94 (MQ=60)

                                                                     GTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCA                          >  SRR4302540.10130745/1‑94 (MQ=60)

                                                                     GTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCA                          >  SRR4302540.4614604/1‑94 (MQ=60)

                                                                     GTCCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGGCCAGCCGCGCATCGGCA                          >  SRR4302540.1007558/1‑94 (MQ=60)

                                                                       CCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAAT                        >  SRR4302540.9111100/1‑94 (MQ=60)

                                                                       CCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAAT                        <  SRR4302540.992380/94‑1 (MQ=60)

                                                                       CCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAAT                        <  SRR4302540.7700222/94‑1 (MQ=60)

                                                                       CCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAAT                        <  SRR4302540.1454319/94‑1 (MQ=60)

                                                                       CCACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTCACCAGTCATGCAAATCAGCGGGATGGAGTCCAGCCGCGAATAGGCAAT                        >  SRR4302540.4692527/1‑94 (MQ=60)

                                                                        CACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATG                       >  SRR4302540.9429615/1‑94 (MQ=60)

                                                                         ACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGG                      >  SRR4302540.8276548/1‑94 (MQ=60)

                                                                         ACTTCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGG                      >  SRR4302540.2474358/1‑94 (MQ=60)

                                                                            TCCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGG                   >  SRR4302540.3667444/1‑94 (MQ=60)

                                                                             CCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGT                  >  SRR4302540.2746403/1‑94 (MQ=60)

                                                                             CCTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGC                  >  SRR4302540.2010621/1‑94 (MQ=60)

                                                                              CTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTC                 >  SRR4302540.1398957/1‑94 (MQ=60)

                                                                              CTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTC                 <  SRR4302540.7771330/94‑1 (MQ=60)

                                                                              CTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTC                 <  SRR4302540.498519/94‑1 (MQ=60)

                                                                              CTGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTC                 >  SRR4302540.5910153/1‑94 (MQ=60)

                                                                               TGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCA                >  SRR4302540.4597580/1‑94 (MQ=60)

                                                                               TGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCA                >  SRR4302540.3236751/1‑94 (MQ=60)

                                                                               CGGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCA                <  SRR4302540.2953514/94‑1 (MQ=60)

                                                                                GGAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCAC               <  SRR4302540.5303534/94‑1 (MQ=60)

                                                                                 GAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACC              >  SRR4302540.6046987/1‑94 (MQ=60)

                                                                                 GAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACC              <  SRR4302540.10133779/94‑1 (MQ=60)

                                                                                 GAAGGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACC              >  SRR4302540.9109796/1‑94 (MQ=60)

                                                                                    GGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGG           <  SRR4302540.9627534/94‑1 (MQ=60)

                                                                                    GGCGTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGG           <  SRR4302540.5925212/94‑1 (MQ=60)

                                                                                      CGTTGGTACCGATCATCGAGGCGGTAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTT         <  SRR4302540.3346046/94‑1 (MQ=60)

                                                                                       GTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTGCAGCCGCGCATCGGCAATGGCGGTCAACAGGATA        >  SRR4302540.297234/1‑94 (MQ=60)

                                                                                       GTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCCATGGCGGTCACCAGGTTA        >  SRR4302540.7658360/1‑94 (MQ=60)

                                                                                       GTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTG        >  SRR4302540.6168413/1‑94 (MQ=60)

                                                                                       GTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTA        >  SRR4302540.7658359/1‑94 (MQ=60)

                                                                                       GTCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTA        >  SRR4302540.8685440/1‑94 (MQ=60)

                                                                                        TCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAG       <  SRR4302540.2502457/94‑1 (MQ=60)

                                                                                        TCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAG       <  SRR4302540.1095265/94‑1 (MQ=60)

                                                                                        TCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAG       >  SRR4302540.8368967/1‑94 (MQ=60)

                                                                                        GCGGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAG       <  SRR4302540.3897107/94‑1 (MQ=60)

                                                                                          GGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTC     >  SRR4302540.2500115/1‑94 (MQ=60)

                                                                                          GGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTC     >  SRR4302540.3929764/1‑94 (MQ=60)

                                                                                          GGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTC     <  SRR4302540.1370305/94‑1 (MQ=60)

                                                                                          GGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTC     >  SRR4302540.333723/1‑94 (MQ=60)

                                                                                          GGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGGTAGTC     >  SRR4302540.3321656/1‑94 (MQ=60)

                                                                                          GGTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGACGCGCATCGGCAATGGCGGTCACCAGGTTAGTC     >  SRR4302540.9803425/1‑94 (MQ=60)

                                                                                           GTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGGTGGATGCCCGCCGCGCACCGGCATGGGCGGTCACCcgggtactcc    >  SRR4302540.2683651/1‑84 (MQ=51)

                                                                                           GTACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCG    <  SRR4302540.6461093/94‑1 (MQ=60)

                                                                                            TACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGC   >  SRR4302540.3509325/1‑94 (MQ=60)

                                                                                            TACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGC   >  SRR4302540.8540825/1‑94 (MQ=60)

                                                                                            TACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGC   >  SRR4302540.2655624/1‑94 (MQ=60)

                                                                                            TACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGC   >  SRR4302540.4720683/1‑94 (MQ=60)

                                                                                             ACCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGTGCACCAGGTTAGTCGGA  >  SRR4302540.9091171/1‑94 (MQ=60)

CTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGAGATGCCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCA  >  NC_000913/3849965‑3850151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`