Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,851,006 A→G intergenic (‑391/‑537) ivbL ← / → emrD ilvB operon leader peptide/2‑module integral membrane pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,851,0060AG95.5% 1744.3 / 45.0 594intergenic (‑391/‑537)ivbL/emrDilvB operon leader peptide/2‑module integral membrane pump
Reads supporting (aligned to +/- strand):  ref base A (14/13);  new base G (251/316);  total (265/329)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 628 total aligned reads displayed.

GTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGA  >  NC_000913/3850916‑3851098
                                                                                          |                                                                                            
 tCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTa                                                                                           >  1:4395611/1‑93 (MQ=255)
 tCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTa                                                                                           <  2:1700149/93‑1 (MQ=255)
  ccAAATATTACTGTTTATTAATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTaa                                                                                          <  2:241630/93‑1 (MQ=255)
   cAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAg                                                                                         <  1:1871692/93‑1 (MQ=255)
   cAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAg                                                                                         >  1:5980780/1‑93 (MQ=255)
     aaTATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTc                                                                                       >  2:6113547/1‑93 (MQ=255)
     aaTATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTc                                                                                       <  1:4271895/93‑1 (MQ=255)
     aaTATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTc                                                                                       <  2:5512171/93‑1 (MQ=255)
       tatTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCt                                                                                     <  1:804767/93‑1 (MQ=255)
       tatTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCt                                                                                     >  1:7346497/1‑93 (MQ=255)
         ttACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTgg                                                                                   <  1:75108/93‑1 (MQ=255)
          tACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGc                                                                                  <  2:1999958/93‑1 (MQ=255)
           aCTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCt                                                                                 >  1:4667973/1‑93 (MQ=255)
           aCTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCt                                                                                 <  1:9257395/93‑1 (MQ=255)
            cTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTAGCCGGCGCGCTAAGTCCTGGCTg                                                                                >  1:5072627/1‑93 (MQ=255)
             tGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGa                                                                               >  1:8530167/1‑93 (MQ=255)
              gTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGaa                                                                              >  1:1026424/1‑93 (MQ=255)
              gTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGaa                                                                              >  1:7283189/1‑93 (MQ=255)
              gTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGaa                                                                              <  2:777259/93‑1 (MQ=255)
               tttatttaTACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGaaa                                                                             <  2:9118383/93‑1 (MQ=255)
                ttatttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAAc                                                                            <  2:3468720/93‑1 (MQ=255)
                ttatttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAAc                                                                            <  2:6501736/93‑1 (MQ=255)
                 tatttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACg                                                                           <  1:4012341/93‑1 (MQ=255)
                   tttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACggg                                                                         <  2:3906872/93‑1 (MQ=255)
                   tttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACggg                                                                         >  2:4094906/1‑93 (MQ=255)
                   tttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACggg                                                                         <  1:8295051/93‑1 (MQ=255)
                     tataCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtg                                                                       <  2:1608784/93‑1 (MQ=255)
                     tataCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtg                                                                       <  2:10045650/93‑1 (MQ=255)
                      ataCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtgg                                                                      <  2:4311984/93‑1 (MQ=255)
                      ataCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtgg                                                                      <  1:2498352/93‑1 (MQ=255)
                       taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggt                                                                     <  1:8812315/93‑1 (MQ=255)
                       taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggt                                                                     <  2:3100553/93‑1 (MQ=255)
                       taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggt                                                                     >  1:1336449/1‑93 (MQ=255)
                       taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggt                                                                     <  1:2880878/93‑1 (MQ=255)
                       taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggg                                                                     >  1:8462122/1‑92 (MQ=255)
                       taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTAGCTGAAACGggcggc                                                                     >  1:6654607/1‑92 (MQ=255)
                        aCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGgtggtg                                                                    >  1:6185232/1‑93 (MQ=255)
                        aCAGTAAACTTCTATAATATCACCGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGATGAAACGggtggtc                                                                    >  2:6719651/1‑92 (MQ=255)
                          aGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGcc                                                                  >  2:9309285/1‑93 (MQ=255)
                          aGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGCGcc                                                                  >  2:2656574/1‑93 (MQ=255)
                             aaaCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTc                                                               >  1:571945/1‑93 (MQ=255)
                              aaCTTCTATAATATCACTGTACGCATTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCa                                                              >  2:1550912/1‑93 (MQ=255)
                              aaCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCa                                                              >  1:10001309/1‑93 (MQ=255)
                               aCTTCTATAATATCCCTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAg                                                             <  1:1731055/93‑1 (MQ=255)
                               aCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTTGCTGAAACGGGTGGTGCCGTCAg                                                             >  1:4810536/1‑93 (MQ=255)
                               aCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAg                                                             <  2:4824230/93‑1 (MQ=255)
                                cTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAgc                                                            <  2:7593407/93‑1 (MQ=255)
                                cTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAgc                                                            <  2:5771992/93‑1 (MQ=255)
                                  tCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAgcgc                                                          >  1:3072890/1‑93 (MQ=255)
                                   cTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGcc                                                         >  2:4748251/1‑93 (MQ=255)
                                       aataTCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTaa                                                     >  2:8434539/1‑93 (MQ=255)
                                        atatCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAc                                                    >  2:1311577/1‑93 (MQ=255)
                                        atatCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAATGGGTGGTGCCGTCAGCGCCTTAAc                                                    >  2:5426501/1‑93 (MQ=255)
                                          atCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAccc                                                  <  1:9383506/93‑1 (MQ=255)
                                             aCTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCcgc                                               >  2:2721100/1‑93 (MQ=255)
                                               tGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCcgcgg                                             >  1:3761587/1‑92 (MQ=255)
                                                 tACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGa                                           <  2:6314296/93‑1 (MQ=255)
                                                 tACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGa                                           >  2:2896421/1‑93 (MQ=255)
                                                   cGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGGCCTGGCTGAAGCGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGc                                         <  1:9114127/93‑1 (MQ=255)
                                                    gCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGca                                        <  1:6550203/93‑1 (MQ=255)
                                                    gCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTAGTGCCGTCAGCGCCTTAACCCCGCGTGAGca                                        <  2:5980779/93‑1 (MQ=255)
                                                      aaTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGcaca                                      >  1:7007346/1‑93 (MQ=255)
                                                      aaTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGcaca                                      >  1:6884202/1‑93 (MQ=255)
                                                       aTGTGTTATGCTGGGGCCGCACCGTTCCCCGGCGCGCTAAGTCCTGCCTGAAACGCGTGGTGCCGTCAGCGCCTTACCCCCGCGTGAGCacac                                     <  2:8902836/93‑1 (MQ=255)
                                                       aTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCacac                                     <  2:7948282/93‑1 (MQ=255)
                                                       aTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCacac                                     >  1:9889406/1‑93 (MQ=255)
                                                        tgtgTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACt                                    >  2:366576/1‑93 (MQ=255)
                                                        tgtgTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACt                                    >  2:6946779/1‑93 (MQ=255)
                                                         tggtTATGCGGGTGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtg                                   <  1:4919911/91‑1 (MQ=255)
                                                         gtgtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtg                                   >  1:3603675/1‑93 (MQ=255)
                                                         gtgtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtg                                   <  2:6758871/93‑1 (MQ=255)
                                                           gtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtgtg                                 >  2:3249360/1‑93 (MQ=255)
                                                            ttATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTgtgt                                >  1:9686958/1‑93 (MQ=255)
                                                            ttATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTgtgt                                <  2:4948854/93‑1 (MQ=255)
                                                              aTGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTa                              <  1:7724468/93‑1 (MQ=255)
                                                               tGCGGTGGCCGCATCGTTGCCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCCCACTGTGTTAt                             <  2:2160201/93‑1 (MQ=255)
                                                                   ggggCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTca                         >  1:1442064/1‑93 (MQ=255)
                                                                    gggCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaa                        <  1:8968352/93‑1 (MQ=255)
                                                                    gggCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaa                        <  2:7360273/93‑1 (MQ=255)
                                                                        cGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAg                    >  1:2319358/1‑93 (MQ=255)
                                                                         gCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGc                   <  2:1107808/93‑1 (MQ=255)
                                                                          cATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGca                  <  1:7882492/93‑1 (MQ=255)
                                                                          cATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGca                  >  2:3329190/1‑93 (MQ=255)
                                                                           aTCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGcac                 >  2:7094475/1‑93 (MQ=255)
                                                                           aTCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGcac                 <  2:9588944/93‑1 (MQ=255)
                                                                           aTCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGcac                 <  1:8766009/93‑1 (MQ=255)
                                                                            tCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGcaca                >  2:6412397/1‑93 (MQ=255)
                                                                             cGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACaa               <  2:5288117/93‑1 (MQ=255)
                                                                             cGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGGGTTATGTCAACAAGCACaa               <  1:5366985/93‑1 (MQ=255)
                                                                              gTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAc              >  1:5853112/1‑93 (MQ=255)
                                                                                 aCCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGtt           <  1:3390702/93‑1 (MQ=255)
                                                                                  cccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGttt          <  1:5531022/93‑1 (MQ=255)
                                                                                  cccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGttt          >  2:8659966/1‑93 (MQ=255)
                                                                                   ccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTtc         >  2:8214062/1‑93 (MQ=255)
                                                                                   ccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTtc         <  2:1865110/93‑1 (MQ=255)
                                                                                     ggCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTtctc       >  1:1982169/1‑93 (MQ=255)
                                                                                      gcgcgcTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTcc      <  1:3254373/93‑1 (MQ=255)
                                                                                       cgcgCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCt     <  1:1146702/93‑1 (MQ=255)
                                                                                          aCTANGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTga  >  1:2637299/1‑93 (MQ=255)
                                                                                          aCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTga  <  2:4552181/93‑1 (MQ=255)
                                                                                          |                                                                                            
GTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGA  >  NC_000913/3850916‑3851098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 339 total aligned reads displayed.
TTTGTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  NC_000913/3850913‑3851099
                                                                                             |                                                                                             
TTTGTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCCTCGTTAGCCGGCGCG                                                                                               >  SRR4302540.4929543/1‑94 (MQ=60)
 TTGTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGC                                                                                              >  SRR4302540.6716395/1‑94 (MQ=60)
   GTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTA                                                                                            >  SRR4302540.4443302/1‑94 (MQ=60)
    TCCAAATATTACTTTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAA                                                                                           <  SRR4302540.88709/94‑1 (MQ=60)
    TCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAA                                                                                           <  SRR4302540.7691062/94‑1 (MQ=60)
    TCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAA                                                                                           <  SRR4302540.8562777/94‑1 (MQ=60)
     CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGTCCGCATCGTTACCCGGCGCGCTAAG                                                                                          <  SRR4302540.4812786/94‑1 (MQ=60)
     CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          <  SRR4302540.6856603/94‑1 (MQ=60)
     CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          <  SRR4302540.8921597/94‑1 (MQ=60)
      CAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGT                                                                                         >  SRR4302540.3412274/1‑94 (MQ=60)
      CAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGT                                                                                         >  SRR4302540.8264787/1‑94 (MQ=60)
      CAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGT                                                                                         >  SRR4302540.1181595/1‑94 (MQ=60)
       AAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTC                                                                                        >  SRR4302540.5813808/1‑94 (MQ=60)
        AATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCC                                                                                       <  SRR4302540.6800525/94‑1 (MQ=60)
        AATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCC                                                                                       <  SRR4302540.4318355/94‑1 (MQ=60)
        AATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCC                                                                                       <  SRR4302540.5859816/94‑1 (MQ=60)
         ATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCT                                                                                      <  SRR4302540.4433407/94‑1 (MQ=60)
         ATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCT                                                                                      <  SRR4302540.7188617/94‑1 (MQ=60)
          TATTACTGTTTATTTATACAGTAAACTTCTATAATATCTCTGTACGCAATGTGTTATGCGCGGGCCGCATCGTTACCCCGCGCGCTCAGTCCTG                                                                                     >  SRR4302540.9002520/1‑94 (MQ=60)
          TATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTG                                                                                     >  SRR4302540.6685373/1‑94 (MQ=60)
          TATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTG                                                                                     <  SRR4302540.5215417/94‑1 (MQ=60)
          TATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTG                                                                                     <  SRR4302540.813314/94‑1 (MQ=60)
           ATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGG                                                                                    >  SRR4302540.9210153/1‑94 (MQ=60)
            TTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGC                                                                                   <  SRR4302540.2277200/94‑1 (MQ=60)
            TTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGC                                                                                   <  SRR4302540.75885/94‑1 (MQ=60)
             TACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCT                                                                                  >  SRR4302540.4717936/1‑94 (MQ=60)
             TACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCT                                                                                  >  SRR4302540.9328578/1‑94 (MQ=60)
              ACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTAGCCGGCGCGCTAAGTCCTGGCTG                                                                                 >  SRR4302540.5127464/1‑94 (MQ=60)
              ACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTG                                                                                 >  SRR4302540.8210388/1‑94 (MQ=60)
               CTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGTCGCGCTAAGTCCTGGCTGA                                                                                >  SRR4302540.9893413/1‑94 (MQ=60)
               CTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGA                                                                                >  SRR4302540.4916221/1‑94 (MQ=60)
                TGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGCA                                                                               >  SRR4302540.2765216/1‑94 (MQ=60)
                TGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAA                                                                               >  SRR4302540.1036837/1‑94 (MQ=60)
                TGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAA                                                                               >  SRR4302540.7364465/1‑94 (MQ=60)
                TGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAA                                                                               <  SRR4302540.8051395/94‑1 (MQ=60)
                TGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAA                                                                               >  SRR4302540.3108813/1‑94 (MQ=60)
                TGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAA                                                                               >  SRR4302540.1036835/1‑94 (MQ=60)
                  TTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGTGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAAC                                                                             >  SRR4302540.6082792/1‑94 (MQ=60)
                  TTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAAC                                                                             <  SRR4302540.10113734/94‑1 (MQ=60)
                   TTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACG                                                                            <  SRR4302540.4634463/94‑1 (MQ=60)
                   TTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACG                                                                            >  SRR4302540.2556578/1‑94 (MQ=60)
                    TATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGG                                                                           <  SRR4302540.4055554/94‑1 (MQ=60)
                     ATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGG                                                                          >  SRR4302540.7612807/1‑94 (MQ=60)
                     ATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGG                                                                          >  SRR4302540.2997934/1‑94 (MQ=60)
                       TTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGTGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTG                                                                        <  SRR4302540.4965268/94‑1 (MQ=60)
                       TAATACACTAAACTTCTATAATATCAATGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGCCCTGGCTGAAACGGGTG                                                                        <  SRR4302540.4965267/94‑1 (MQ=60)
                        TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       <  SRR4302540.3009239/94‑1 (MQ=60)
                        TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       <  SRR4302540.6322274/94‑1 (MQ=60)
                        TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       >  SRR4302540.6973237/1‑94 (MQ=60)
                        TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       <  SRR4302540.9240463/94‑1 (MQ=60)
                        TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       >  SRR4302540.5796239/1‑94 (MQ=60)
                        TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       <  SRR4302540.1451120/94‑1 (MQ=60)
                         ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGT                                                                      >  SRR4302540.1350440/1‑94 (MQ=60)
                         ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGT                                                                      <  SRR4302540.2525290/94‑1 (MQ=60)
                         ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGT                                                                      <  SRR4302540.3449771/94‑1 (MQ=60)
                         ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGT                                                                      <  SRR4302540.4400493/94‑1 (MQ=60)
                         ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGG                                                                      >  SRR4302540.638971/1‑94 (MQ=60)
                         ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGG                                                                      >  SRR4302540.8560379/1‑94 (MQ=60)
                         ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTAGCTGAAACGGGcggc                                                                      >  SRR4302540.6728207/1‑90 (MQ=60)
                          TACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTG                                                                     <  SRR4302540.2912218/94‑1 (MQ=60)
                          TACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTG                                                                     <  SRR4302540.6937055/94‑1 (MQ=60)
                          TACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTG                                                                     >  SRR4302540.6254117/1‑94 (MQ=60)
                          AACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTG                                                                     <  SRR4302540.7904748/94‑1 (MQ=60)
                           ACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGC                                                                    <  SRR4302540.10035269/94‑1 (MQ=60)
                           ACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGC                                                                    >  SRR4302540.3105525/1‑94 (MQ=60)
                            CAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCC                                                                   <  SRR4302540.1366193/94‑1 (MQ=60)
                            CAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCC                                                                   <  SRR4302540.3843129/94‑1 (MQ=60)
                              GTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGT                                                                 >  SRR4302540.2248065/1‑94 (MQ=60)
                               TAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTC                                                                >  SRR4302540.3551374/1‑94 (MQ=60)
                               TAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTC                                                                <  SRR4302540.2810524/94‑1 (MQ=60)
                               TAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTC                                                                >  SRR4302540.4465577/1‑94 (MQ=60)
                                AATCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCA                                                               <  SRR4302540.5659131/94‑1 (MQ=60)
                                 AACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTTGCTGAAACGGGTGGTGCCGTCAG                                                              >  SRR4302540.4862024/1‑94 (MQ=60)
                                 AACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAG                                                              >  SRR4302540.6653851/1‑94 (MQ=60)
                                  ACTTCTATAATATCCCTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGC                                                             <  SRR4302540.1749738/94‑1 (MQ=60)
                                  ACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGC                                                             <  SRR4302540.4643418/94‑1 (MQ=60)
                                    TTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGC                                                           >  SRR4302540.192696/1‑94 (MQ=60)
                                     TCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCC                                                          >  SRR4302540.9967772/1‑94 (MQ=60)
                                      CTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCT                                                         <  SRR4302540.1029953/94‑1 (MQ=60)
                                       TATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTT                                                        >  SRR4302540.1826554/1‑94 (MQ=60)
                                       TATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTT                                                        >  SRR4302540.9571715/1‑94 (MQ=60)
                                       TATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTT                                                        <  SRR4302540.3348584/94‑1 (MQ=60)
                                        ATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGGGCCTTA                                                       >  SRR4302540.6183661/1‑94 (MQ=60)
                                        ATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTA                                                       <  SRR4302540.6042936/94‑1 (MQ=60)
                                          AATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAC                                                     >  SRR4302540.3864797/1‑94 (MQ=60)
                                          AATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAC                                                     >  SRR4302540.9141653/1‑94 (MQ=60)
                                           ATATCACTGTACGCAATGTGTTATGCGGGGGGCGAATCGTTCCCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGCGCCGTCCGCGCCTTAACC                                                    >  SRR4302540.8751391/1‑94 (MQ=60)
                                           ATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACC                                                    <  SRR4302540.1375103/94‑1 (MQ=60)
                                            TATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCC                                                   >  SRR4302540.5339372/1‑94 (MQ=60)
                                            TATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCC                                                   >  SRR4302540.7546841/1‑94 (MQ=60)
                                             ATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCC                                                  <  SRR4302540.2812777/94‑1 (MQ=60)
                                             ATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCC                                                  <  SRR4302540.10157573/94‑1 (MQ=60)
                                             ATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCC                                                  <  SRR4302540.7380389/94‑1 (MQ=60)
                                             ATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCC                                                  <  SRR4302540.1840386/94‑1 (MQ=60)
                                             ATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACACGGCGAGCTCAGTCGTGGCTGAAACGGGTGGTGCCGTCCGCGCATTAACCAC                                                  >  SRR4302540.6143528/1‑94 (MQ=60)
                                              TCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCG                                                 >  SRR4302540.9768972/1‑94 (MQ=60)
                                               CACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGC                                                <  SRR4302540.8426962/94‑1 (MQ=60)
                                               CACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGC                                                >  SRR4302540.3969423/1‑94 (MQ=60)
                                                ACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAACCGGGTGGTGCCGTCAGCGCCTTAACCCCGCG                                               >  SRR4302540.509109/1‑94 (MQ=60)
                                                ACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCG                                               >  SRR4302540.2488537/1‑94 (MQ=60)
                                                ACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCG                                               <  SRR4302540.8631746/94‑1 (MQ=60)
                                                 CTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGT                                              <  SRR4302540.1255216/94‑1 (MQ=60)
                                                 CTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGT                                              <  SRR4302540.7415756/94‑1 (MQ=60)
                                                 CTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGG                                              >  SRR4302540.139510/1‑94 (MQ=60)
                                                  TGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTG                                             >  SRR4302540.9859814/1‑94 (MQ=60)
                                                   GTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGA                                            <  SRR4302540.9215285/94‑1 (MQ=60)
                                                    TACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAG                                           <  SRR4302540.5473807/94‑1 (MQ=60)
                                                     ACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTaagcgctagatcagaagagcggttcagc                                          >  SRR4302540.8126467/1‑66 (MQ=60)
                                                     ACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCTTGAGC                                          >  SRR4302540.6093751/1‑94 (MQ=60)
                                                     ACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGC                                          >  SRR4302540.8635363/1‑94 (MQ=60)
                                                      CGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCA                                         >  SRR4302540.6617357/1‑94 (MQ=60)
                                                      CGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCA                                         <  SRR4302540.4143077/94‑1 (MQ=60)
                                                      CGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCA                                         >  SRR4302540.8787640/1‑94 (MQ=60)
                                                       GCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCAC                                        <  SRR4302540.1924591/94‑1 (MQ=60)
                                                       GCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCAC                                        <  SRR4302540.6622857/94‑1 (MQ=60)
                                                       GCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCAC                                        <  SRR4302540.9420166/94‑1 (MQ=60)
                                                       GCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCAC                                        <  SRR4302540.3313423/94‑1 (MQ=60)
                                                       GCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCAC                                        <  SRR4302540.5554880/94‑1 (MQ=60)
                                                        CAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACA                                       >  SRR4302540.7085121/1‑94 (MQ=60)
                                                        CAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACA                                       >  SRR4302540.2586189/1‑94 (MQ=60)
                                                         AATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACAC                                      >  SRR4302540.2153397/1‑94 (MQ=60)
                                                         AATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACAC                                      >  SRR4302540.1154328/1‑94 (MQ=60)
                                                         AATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACAC                                      >  SRR4302540.1519044/1‑94 (MQ=60)
                                                         AATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACAC                                      >  SRR4302540.10004874/1‑94 (MQ=60)
                                                          ATTTGTCATGCGGAGCCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACT                                     <  SRR4302540.8417343/94‑1 (MQ=60)
                                                          ATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACT                                     <  SRR4302540.3753949/94‑1 (MQ=60)
                                                          ATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACT                                     <  SRR4302540.5649859/94‑1 (MQ=60)
                                                          ATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACT                                     <  SRR4302540.2149385/94‑1 (MQ=60)
                                                          ATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACT                                     <  SRR4302540.7253647/94‑1 (MQ=60)
                                                           TGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTG                                    >  SRR4302540.3643053/1‑94 (MQ=60)
                                                           TGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTG                                    >  SRR4302540.9409732/1‑94 (MQ=60)
                                                            tgGTTATGCGGGTGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGT                                   <  SRR4302540.4972911/92‑1 (MQ=60)
                                                            GTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGT                                   <  SRR4302540.8688241/94‑1 (MQ=60)
                                                             TGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTG                                  >  SRR4302540.10130965/1‑94 (MQ=60)
                                                             TGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGGGAGCACACTGTG                                  >  SRR4302540.7800029/1‑94 (MQ=60)
                                                              gtgagGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGT                                 <  SRR4302540.7003747/89‑1 (MQ=60)
                                                              GTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGT                                 >  SRR4302540.9290440/1‑94 (MQ=60)
                                                              GTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGT                                 >  SRR4302540.9800913/1‑94 (MQ=60)
                                                              GTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGT                                 >  SRR4302540.8624876/1‑94 (MQ=60)
                                                               TTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTT                                <  SRR4302540.8385369/94‑1 (MQ=60)
                                                                TATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTA                               <  SRR4302540.9499121/94‑1 (MQ=60)
                                                                 ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302540.4814170/94‑1 (MQ=60)
                                                                 ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302540.5012097/94‑1 (MQ=60)
                                                                 ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302540.7814481/94‑1 (MQ=60)
                                                                 ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302540.3741730/94‑1 (MQ=60)
                                                                 ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302540.5012098/94‑1 (MQ=60)
                                                                  TGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATG                             <  SRR4302540.9376973/94‑1 (MQ=60)
                                                                  TGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATG                             >  SRR4302540.84954/1‑94 (MQ=60)
                                                                    CGGGGGCCGCATCGTTGCCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTC                           <  SRR4302540.1717718/94‑1 (MQ=60)
                                                                    CGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTC                           <  SRR4302540.3190453/94‑1 (MQ=60)
                                                                     GGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCA                          <  SRR4302540.1722401/94‑1 (MQ=60)
                                                                       GGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAAC                        <  SRR4302540.5687636/94‑1 (MQ=60)
                                                                       GGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAAC                        <  SRR4302540.9074725/94‑1 (MQ=60)
                                                                       GGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCATGAGCACACTGTGTTATGTCAAC                        <  SRR4302540.4072709/94‑1 (MQ=60)
                                                                          CCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAG                     >  SRR4302540.2344324/1‑94 (MQ=60)
                                                                          CCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAC                     >  SRR4302540.7576201/1‑94 (MQ=60)
                                                                            GCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCA                   >  SRR4302540.3800742/1‑94 (MQ=60)
                                                                            GCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCA                   >  SRR4302540.5194462/1‑94 (MQ=60)
                                                                            GCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCA                   >  SRR4302540.8868207/1‑94 (MQ=60)
                                                                             CATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCAC                  <  SRR4302540.7974531/94‑1 (MQ=60)
                                                                             CATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCAC                  <  SRR4302540.2384304/94‑1 (MQ=60)
                                                                              ATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACA                 >  SRR4302540.4334910/1‑94 (MQ=60)
                                                                              ATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACA                 >  SRR4302540.5987879/1‑94 (MQ=60)
                                                                                CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               >  SRR4302540.5917847/1‑94 (MQ=60)
                                                                                CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               <  SRR4302540.7890340/94‑1 (MQ=60)
                                                                                CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               <  SRR4302540.1957021/94‑1 (MQ=60)
                                                                                CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               <  SRR4302540.7446944/94‑1 (MQ=60)
                                                                                CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               <  SRR4302540.9283258/94‑1 (MQ=60)
                                                                                CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGGGTTATGTCAACAAGCACAAC               <  SRR4302540.5425960/94‑1 (MQ=60)
                                                                                 GTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACG              >  SRR4302540.5472096/1‑94 (MQ=60)
                                                                                  TTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGT             <  SRR4302540.3216136/94‑1 (MQ=60)
                                                                                  TTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGT             >  SRR4302540.7253681/1‑94 (MQ=60)
                                                                                  TTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGT             <  SRR4302540.8179895/94‑1 (MQ=60)
                                                                                   TACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTT            >  SRR4302540.890785/1‑94 (MQ=60)
                                                                                   TACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTT            >  SRR4302540.766152/1‑94 (MQ=60)
                                                                                    ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAGCCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAGCGTTT           <  SRR4302540.6998191/94‑1 (MQ=60)
                                                                                    ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTT           <  SRR4302540.6033014/94‑1 (MQ=60)
                                                                                    ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTT           <  SRR4302540.3427697/94‑1 (MQ=60)
                                                                                    ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTT           <  SRR4302540.7053519/94‑1 (MQ=60)
                                                                                     CCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTC          <  SRR4302540.424563/94‑1 (MQ=60)
                                                                                     CCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTC          <  SRR4302540.8898676/94‑1 (MQ=60)
                                                                                     CCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTC          <  SRR4302540.856131/94‑1 (MQ=60)
                                                                                     CCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTC          <  SRR4302540.5591937/94‑1 (MQ=60)
                                                                                     CCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTC          <  SRR4302540.3367296/94‑1 (MQ=60)
                                                                                      CCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCT         <  SRR4302540.738880/94‑1 (MQ=60)
                                                                                      CCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCT         <  SRR4302540.2590382/94‑1 (MQ=60)
                                                                                       CGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTC        >  SRR4302540.2003662/1‑94 (MQ=60)
                                                                                         GCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCT      <  SRR4302540.3289847/94‑1 (MQ=60)
                                                                                          CGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTT     <  SRR4302540.1055466/94‑1 (MQ=60)
                                                                                          CGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTT     <  SRR4302540.1887599/94‑1 (MQ=60)
                                                                                          CGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTT     <  SRR4302540.7541475/94‑1 (MQ=60)
                                                                                          CGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTT     <  SRR4302540.1158669/94‑1 (MQ=60)
                                                                                          CGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTT     >  SRR4302540.4931659/1‑94 (MQ=60)
                                                                                          CGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTT     <  SRR4302540.3492593/94‑1 (MQ=60)
                                                                                           GCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTG    <  SRR4302540.4799994/94‑1 (MQ=60)
                                                                                            CACTANGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGA   >  SRR4302540.2665863/1‑94 (MQ=60)
                                                                                             GCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  SRR4302540.9446658/1‑94 (MQ=60)
                                                                                             GCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  SRR4302540.5579414/1‑94 (MQ=60)
                                                                                             GCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  SRR4302540.3522828/1‑94 (MQ=60)
                                                                                             GCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  <  SRR4302540.7106906/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTGTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  NC_000913/3850913‑3851099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑