Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,851,006 A→G intergenic (‑391/‑537) ivbL ← / → emrD ilvB operon leader peptide/2‑module integral membrane pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,851,0060AG100.0% 791.0 / NA 245intergenic (‑391/‑537)ivbL/emrDilvB operon leader peptide/2‑module integral membrane pump
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (154/91);  total (154/91)

Only 100 of 254 total aligned reads displayed.

TCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCT  >  NC_000913/3850917‑3851095
                                                                                         |                                                                                         
tCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTa                                                                                        >  2:4921679/1‑93 (MQ=255)
tCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTa                                                                                        >  2:6090727/1‑93 (MQ=255)
 ccAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTaa                                                                                       <  1:4687223/93‑1 (MQ=255)
 ccAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTaa                                                                                       <  1:3534001/93‑1 (MQ=255)
 ccAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTaa                                                                                       <  1:2254601/93‑1 (MQ=255)
  cAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAg                                                                                      >  1:5039040/1‑93 (MQ=255)
  cAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAg                                                                                      >  1:3937381/1‑93 (MQ=255)
  cAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAg                                                                                      >  1:3263322/1‑93 (MQ=255)
  cAAATATTACTGTTTATTTATACAGTAAACTTCGATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAg                                                                                      <  2:3390581/93‑1 (MQ=255)
   aaaTATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGt                                                                                     >  1:4035256/1‑93 (MQ=255)
    aaTATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTc                                                                                    >  1:1014200/1‑93 (MQ=255)
    aaTATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTc                                                                                    <  2:528405/93‑1 (MQ=255)
     atatTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTcc                                                                                   >  1:3142691/1‑93 (MQ=255)
      tatTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCt                                                                                  >  1:1543705/1‑93 (MQ=255)
       atTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTg                                                                                 >  2:3063162/1‑93 (MQ=255)
        ttACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTgg                                                                                >  1:4774060/1‑93 (MQ=255)
         tACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGc                                                                               <  2:6187579/93‑1 (MQ=255)
         tACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGc                                                                               <  2:5174931/93‑1 (MQ=255)
          aCTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCt                                                                              >  1:1440120/1‑93 (MQ=255)
           cTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTg                                                                             >  2:1993495/1‑93 (MQ=255)
           cTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTg                                                                             >  1:4307995/1‑93 (MQ=255)
               ttatttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAAc                                                                         >  1:4364722/1‑93 (MQ=255)
                 atttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACgg                                                                       >  1:1955426/1‑93 (MQ=255)
                  tttatACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACggg                                                                      <  2:2083207/93‑1 (MQ=255)
                   ttatACAGTAAACTTCTATAATATCACTGTAGGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggt                                                                     >  2:3905971/1‑93 (MQ=255)
                    tataCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGTGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtg                                                                    >  2:6309747/1‑93 (MQ=255)
                     ataCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtgg                                                                   >  1:4412885/1‑93 (MQ=255)
                     ataCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtgg                                                                   >  1:5439725/1‑93 (MQ=255)
                      taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggt                                                                  >  2:48072/1‑93 (MQ=255)
                      taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggt                                                                  <  2:5053414/93‑1 (MQ=255)
                      taCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGggtggt                                                                  <  1:3677404/93‑1 (MQ=255)
                       aCAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGgtggtg                                                                 >  1:4272199/1‑93 (MQ=255)
                        cAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGc                                                                <  1:2955164/93‑1 (MQ=255)
                        cAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGc                                                                >  1:1501888/1‑93 (MQ=255)
                         aGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGcc                                                               >  1:1193952/1‑93 (MQ=255)
                          gTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCg                                                              >  1:828826/1‑93 (MQ=255)
                           tAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGt                                                             <  2:184221/93‑1 (MQ=255)
                            aaaCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTc                                                            <  2:5461287/93‑1 (MQ=255)
                              aCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCGGCATCGTTACCCGGCGCGCTCAGTCCTGGCTGAAACGGGTGGTGCCGTCAg                                                          >  2:1230267/1‑93 (MQ=255)
                              aCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAg                                                          >  1:4520865/1‑93 (MQ=255)
                              aCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAg                                                          >  1:5255292/1‑93 (MQ=255)
                              aCTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAg                                                          >  1:172593/1‑93 (MQ=255)
                                 tCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAgcgc                                                       >  1:611890/1‑93 (MQ=255)
                                  cTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGcc                                                      >  1:4990069/1‑93 (MQ=255)
                                   tataATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCt                                                     >  2:3147867/1‑93 (MQ=255)
                                   tataATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCt                                                     >  1:2711892/1‑93 (MQ=255)
                                    ataataTCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCtt                                                    <  1:3366958/93‑1 (MQ=255)
                                      aataTCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTaa                                                  <  1:5720615/93‑1 (MQ=255)
                                      aataTCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTaa                                                  >  2:4775786/1‑93 (MQ=255)
                                       atatCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAc                                                 >  1:2813988/1‑93 (MQ=255)
                                        aatCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAcc                                                <  1:3109885/92‑1 (MQ=255)
                                         atCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAccc                                               >  1:701369/1‑93 (MQ=255)
                                         atCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAccc                                               >  2:2360517/1‑93 (MQ=255)
                                          tCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAcccc                                              <  2:464270/93‑1 (MQ=255)
                                            aCTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCcgc                                            >  1:6141615/1‑93 (MQ=255)
                                            aCTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCcgc                                            >  2:823501/1‑93 (MQ=255)
                                            aCTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCcgc                                            >  2:3751499/1‑93 (MQ=255)
                                              tGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTCAGTCCTGGCTGAAGCGGGTGGTGCCGTCAGCGCCTTAACCCcgtgg                                          >  2:3593054/1‑90 (MQ=255)
                                               gTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTg                                         >  2:242893/1‑93 (MQ=255)
                                                  cGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGc                                      <  1:2407430/93‑1 (MQ=255)
                                                   gCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGca                                     >  1:5872920/1‑93 (MQ=255)
                                                     aaTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGcaca                                   <  2:4707606/93‑1 (MQ=255)
                                                     aaTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGcaca                                   >  2:761393/1‑93 (MQ=255)
                                                     aaTGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGcaca                                   >  2:1087885/1‑93 (MQ=255)
                                                       tgtgTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACt                                 >  1:4124644/1‑93 (MQ=255)
                                                       tgtgTTATGCGGGGGCCGCATCGTTACCCGGAGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACt                                 >  2:4288076/1‑93 (MQ=255)
                                                        gtgtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtg                                <  1:2187318/93‑1 (MQ=255)
                                                        gtgtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtg                                >  1:6011795/1‑93 (MQ=255)
                                                         tgtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtgt                               >  1:2544721/1‑93 (MQ=255)
                                                         tgtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtgt                               <  2:300710/93‑1 (MQ=255)
                                                         tgtTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACtgt                               <  1:884843/93‑1 (MQ=255)
                                                            tATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGtt                            <  2:5719498/93‑1 (MQ=255)
                                                             aTGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTa                           <  1:782324/93‑1 (MQ=255)
                                                              tGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAt                          <  2:4409175/93‑1 (MQ=255)
                                                                 gggggCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTc                       <  1:5395185/93‑1 (MQ=255)
                                                                  ggggCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTca                      >  1:1765340/1‑93 (MQ=255)
                                                                   gggCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaa                     >  2:1295430/1‑93 (MQ=255)
                                                                    ggCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaac                    <  1:5150408/93‑1 (MQ=255)
                                                                    ggCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaac                    <  2:2046831/93‑1 (MQ=255)
                                                                     gCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaaca                   >  2:4138126/1‑93 (MQ=255)
                                                                     gCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaaca                   >  2:1472984/1‑93 (MQ=255)
                                                                      ccGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTcaacaa                  >  2:2800955/1‑93 (MQ=255)
                                                                       cGCATCGTTACCCTGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAg                 >  2:6305486/1‑93 (MQ=255)
                                                                       cGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAg                 >  1:692197/1‑93 (MQ=255)
                                                                        gCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGc                >  1:5682446/1‑93 (MQ=255)
                                                                         cATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGca               <  2:5860991/93‑1 (MQ=255)
                                                                         cATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGca               <  2:900089/93‑1 (MQ=255)
                                                                          aTCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGcac              >  2:3167807/1‑93 (MQ=255)
                                                                           tCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGcaca             >  2:2639924/1‑93 (MQ=255)
                                                                            cGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACaa            <  2:2202621/93‑1 (MQ=255)
                                                                               tACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGt         <  1:5090327/93‑1 (MQ=255)
                                                                                aCCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGtt        <  1:2941668/93‑1 (MQ=255)
                                                                                 cccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGttt       >  1:3832616/1‑93 (MQ=255)
                                                                                 cccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGttt       >  1:1069300/1‑93 (MQ=255)
                                                                                  ccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTtc      <  1:6284710/93‑1 (MQ=255)
                                                                                  ccGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTtc      <  1:2092611/93‑1 (MQ=255)
                                                                                   cGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGATtct     >  2:43120/1‑93 (MQ=255)
                                                                                     gcgcgcTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTcc   <  2:1628332/93‑1 (MQ=255)
                                                                                     gcgcgcTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAAGTTTCTcc   >  2:4088636/1‑93 (MQ=255)
                                                                                      cgcgCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCt  <  1:1128817/93‑1 (MQ=255)
                                                                                         |                                                                                         
TCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCT  >  NC_000913/3850917‑3851095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  NC_000913/3850918‑3851099
                                                                                        |                                                                                             
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          >  SRR4302533.5069550/1‑94 (MQ=60)
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          >  SRR4302533.3961416/1‑94 (MQ=60)
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          <  SRR4302533.2268544/94‑1 (MQ=60)
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          <  SRR4302533.4715631/94‑1 (MQ=60)
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          >  SRR4302533.3283452/1‑94 (MQ=60)
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAG                                                                                          <  SRR4302533.3555777/94‑1 (MQ=60)
 CAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGT                                                                                         >  SRR4302533.4059820/1‑94 (MQ=60)
  AAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTC                                                                                        >  SRR4302533.1020140/1‑94 (MQ=60)
   AATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCC                                                                                       >  SRR4302533.3640460/1‑94 (MQ=60)
   AATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCC                                                                                       >  SRR4302533.3162008/1‑94 (MQ=60)
    ATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCT                                                                                      >  SRR4302533.1553003/1‑94 (MQ=60)
    ATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCT                                                                                      >  SRR4302533.2042644/1‑94 (MQ=60)
     TATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTG                                                                                     >  SRR4302533.4406060/1‑94 (MQ=60)
     TATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTG                                                                                     >  SRR4302533.5812007/1‑94 (MQ=60)
     TATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTG                                                                                     <  SRR4302533.217040/94‑1 (MQ=60)
     TATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTG                                                                                     <  SRR4302533.2034048/94‑1 (MQ=60)
      ATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGG                                                                                    >  SRR4302533.4802988/1‑94 (MQ=60)
      ATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGG                                                                                    <  SRR4302533.5404615/94‑1 (MQ=60)
      ATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGG                                                                                    <  SRR4302533.6305281/94‑1 (MQ=60)
        TACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCT                                                                                  >  SRR4302533.2893356/1‑94 (MQ=60)
        TACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCT                                                                                  >  SRR4302533.1448809/1‑94 (MQ=60)
         ACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTG                                                                                 >  SRR4302533.3003325/1‑94 (MQ=60)
         ACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTG                                                                                 >  SRR4302533.6242702/1‑94 (MQ=60)
         ACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTG                                                                                 >  SRR4302533.4333923/1‑94 (MQ=60)
          CTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGA                                                                                >  SRR4302533.1318408/1‑94 (MQ=60)
          CTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGA                                                                                >  SRR4302533.5718399/1‑94 (MQ=60)
            GTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAA                                                                              <  SRR4302533.5180382/94‑1 (MQ=60)
             TTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAAC                                                                             >  SRR4302533.431258/1‑94 (MQ=60)
             TTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAAC                                                                             >  SRR4302533.4391073/1‑94 (MQ=60)
              TTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACG                                                                            <  SRR4302533.1793570/94‑1 (MQ=60)
               TATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGG                                                                           <  SRR4302533.3959577/94‑1 (MQ=60)
               TATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGG                                                                           >  SRR4302533.1967340/1‑94 (MQ=60)
               TATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGG                                                                           <  SRR4302533.4014146/94‑1 (MQ=60)
                 TTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGT                                                                         <  SRR4302533.4062586/94‑1 (MQ=60)
                  TTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTG                                                                        >  SRR4302533.3889189/1‑94 (MQ=60)
                   TATACAGTAAACTTCTATGATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       >  SRR4302533.4626594/1‑94 (MQ=60)
                   TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       >  SRR4302533.5472484/1‑94 (MQ=60)
                   TATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGG                                                                       >  SRR4302533.4439534/1‑94 (MQ=60)
                    ATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGT                                                                      >  SRR4302533.2275963/1‑94 (MQ=60)
                     TACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTG                                                                     >  SRR4302533.4297912/1‑94 (MQ=60)
                     TACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTG                                                                     <  SRR4302533.3700006/94‑1 (MQ=60)
                     TACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTG                                                                     <  SRR4302533.5167160/94‑1 (MQ=60)
                     TACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGGG                                                                     >  SRR4302533.289197/1‑94 (MQ=60)
                      ACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGC                                                                    >  SRR4302533.1743716/1‑94 (MQ=60)
                      ACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGC                                                                    >  SRR4302533.1510942/1‑94 (MQ=60)
                       CAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCC                                                                   <  SRR4302533.2973234/94‑1 (MQ=60)
                       CAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCC                                                                   >  SRR4302533.318107/1‑94 (MQ=60)
                       CAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCC                                                                   >  SRR4302533.1201130/1‑94 (MQ=60)
                        AGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCG                                                                  >  SRR4302533.833687/1‑94 (MQ=60)
                         GTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGT                                                                 >  SRR4302533.802833/1‑94 (MQ=60)
                           AAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCA                                                               >  SRR4302533.2717795/1‑94 (MQ=60)
                           AAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCA                                                               <  SRR4302533.5381604/94‑1 (MQ=60)
                           AAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCA                                                               >  SRR4302533.2629164/1‑94 (MQ=60)
                           AAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGGGGTGCCGTCA                                                               >  SRR4302533.6341180/1‑94 (MQ=60)
                            AACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAG                                                              >  SRR4302533.173682/1‑94 (MQ=60)
                            AACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAG                                                              >  SRR4302533.5286860/1‑94 (MQ=60)
                            AACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAG                                                              >  SRR4302533.4548239/1‑94 (MQ=60)
                             ACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGC                                                             <  SRR4302533.767177/94‑1 (MQ=60)
                              CTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCG                                                            >  SRR4302533.2227903/1‑94 (MQ=60)
                              CTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCG                                                            >  SRR4302533.1449584/1‑94 (MQ=60)
                              CTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCG                                                            >  SRR4302533.5093551/1‑94 (MQ=60)
                              CTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCG                                                            >  SRR4302533.475440/1‑94 (MQ=60)
                               TTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGC                                                           >  SRR4302533.433292/1‑94 (MQ=60)
                               TTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGC                                                           >  SRR4302533.615487/1‑94 (MQ=60)
                                TCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCC                                                          >  SRR4302533.5020322/1‑94 (MQ=60)
                                TCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCC                                                          >  SRR4302533.4910640/1‑94 (MQ=60)
                                 CTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCT                                                         >  SRR4302533.226309/1‑94 (MQ=60)
                                 CTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCT                                                         >  SRR4302533.2728541/1‑94 (MQ=60)
                                  TATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTT                                                        >  SRR4302533.441122/1‑94 (MQ=60)
                                  TATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTT                                                        >  SRR4302533.698384/1‑94 (MQ=60)
                                  TATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTT                                                        >  SRR4302533.1166137/1‑94 (MQ=60)
                                  TATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTT                                                        <  SRR4302533.5279612/94‑1 (MQ=60)
                                   ATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTA                                                       >  SRR4302533.2274630/1‑94 (MQ=60)
                                   ATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTA                                                       <  SRR4302533.3387754/94‑1 (MQ=60)
                                    TAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGCTACCCGGCGCGCTAAGTACTCGCTGAAACGtgcagagccgtcagagccttca                                                      >  SRR4302533.2658553/1‑72 (MQ=60)
                                     AATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAC                                                     >  SRR4302533.2831307/1‑94 (MQ=60)
                                     AATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAC                                                     <  SRR4302533.5755155/94‑1 (MQ=60)
                                     AATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAAC                                                     >  SRR4302533.502684/1‑94 (MQ=60)
                                       TATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCC                                                   <  SRR4302533.6159067/94‑1 (MQ=60)
                                       TATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCC                                                   >  SRR4302533.705520/1‑94 (MQ=60)
                                       AATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCC                                                   <  SRR4302533.3128999/94‑1 (MQ=60)
                                        ATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCC                                                  <  SRR4302533.151988/94‑1 (MQ=60)
                                         TCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTCCCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCG                                                 <  SRR4302533.3418616/94‑1 (MQ=60)
                                          CACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGC                                                >  SRR4302533.6178603/1‑94 (MQ=60)
                                          CACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGC                                                >  SRR4302533.5614232/1‑94 (MQ=60)
                                           ACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCG                                               <  SRR4302533.5435261/94‑1 (MQ=60)
                                            CTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGT                                              >  SRR4302533.2506060/1‑94 (MQ=60)
                                            CTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGT                                              >  SRR4302533.614885/1‑94 (MQ=60)
                                                ACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGC                                          >  SRR4302533.1290352/1‑94 (MQ=60)
                                                ACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGC                                          <  SRR4302533.33945/94‑1 (MQ=60)
                                                 CGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCA                                         >  SRR4302533.5908417/1‑94 (MQ=60)
                                                 CGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCA                                         <  SRR4302533.2422300/94‑1 (MQ=60)
                                                  GCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTTGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCAC                                        >  SRR4302533.1117962/1‑94 (MQ=60)
                                                    AATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACAC                                      >  SRR4302533.173954/1‑94 (MQ=60)
                                                    AATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACAC                                      >  SRR4302533.5557405/1‑94 (MQ=60)
                                                     ATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACT                                     >  SRR4302533.4149683/1‑94 (MQ=60)
                                                      TGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTG                                    >  SRR4302533.6048216/1‑94 (MQ=60)
                                                       GTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGT                                   <  SRR4302533.2200793/94‑1 (MQ=60)
                                                       GTGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGT                                   >  SRR4302533.2560380/1‑94 (MQ=60)
                                                        TGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTG                                  <  SRR4302533.890041/94‑1 (MQ=60)
                                                        TGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTG                                  <  SRR4302533.1083878/94‑1 (MQ=60)
                                                        TGTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTG                                  <  SRR4302533.4181365/94‑1 (MQ=60)
                                                         GTTATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGT                                 >  SRR4302533.6120438/1‑94 (MQ=60)
                                                           TATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTA                               >  SRR4302533.871183/1‑94 (MQ=60)
                                                            ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302533.786927/94‑1 (MQ=60)
                                                            ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302533.1628649/94‑1 (MQ=60)
                                                            ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              <  SRR4302533.3016187/94‑1 (MQ=60)
                                                            ATGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT                              >  SRR4302533.370033/1‑94 (MQ=60)
                                                             TGCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATG                             <  SRR4302533.858701/94‑1 (MQ=60)
                                                              GCGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGT                            >  SRR4302533.598126/1‑94 (MQ=60)
                                                               CGGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTC                           >  SRR4302533.323856/1‑94 (MQ=60)
                                                                GGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCA                          >  SRR4302533.1776091/1‑94 (MQ=60)
                                                                GGGGGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCA                          <  SRR4302533.5427606/94‑1 (MQ=60)
                                                                   GGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACA                       <  SRR4302533.1045170/94‑1 (MQ=60)
                                                                   GGCCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACA                       <  SRR4302533.5181522/94‑1 (MQ=60)
                                                                     CCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAG                     >  SRR4302533.696286/1‑94 (MQ=60)
                                                                     CCGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAG                     <  SRR4302533.4930701/94‑1 (MQ=60)
                                                                      CGCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGC                    >  SRR4302533.5716771/1‑94 (MQ=60)
                                                                       GCATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGGGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCA                   >  SRR4302533.4122490/1‑94 (MQ=60)
                                                                        CATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCAC                  >  SRR4302533.3306375/1‑94 (MQ=60)
                                                                        CATCGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCAC                  <  SRR4302533.4631038/94‑1 (MQ=60)
                                                                           CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               >  SRR4302533.5103194/1‑94 (MQ=60)
                                                                           CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               <  SRR4302533.3844356/94‑1 (MQ=60)
                                                                           CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               <  SRR4302533.3151139/94‑1 (MQ=60)
                                                                           CGTTACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAAC               <  SRR4302533.2747450/94‑1 (MQ=60)
                                                                              TACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTT            <  SRR4302533.5121083/94‑1 (MQ=60)
                                                                              TACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTT            <  SRR4302533.5889630/94‑1 (MQ=60)
                                                                              TACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGACTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTT            >  SRR4302533.1514128/1‑94 (MQ=60)
                                                                               ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTT           >  SRR4302533.1075597/1‑94 (MQ=60)
                                                                               ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTT           <  SRR4302533.2959633/94‑1 (MQ=60)
                                                                               ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTT           >  SRR4302533.4390387/1‑94 (MQ=60)
                                                                               ACCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTT           >  SRR4302533.3856042/1‑94 (MQ=60)
                                                                                CCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTC          >  SRR4302533.2972725/1‑94 (MQ=60)
                                                                                CCCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTC          >  SRR4302533.70639/1‑94 (MQ=60)
                                                                                 CCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCT         <  SRR4302533.6322211/94‑1 (MQ=60)
                                                                                 CCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCT         >  SRR4302533.4538141/1‑94 (MQ=60)
                                                                                 CCGGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCT         <  SRR4302533.2105499/94‑1 (MQ=60)
                                                                                   GGCGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCC       <  SRR4302533.675828/94‑1 (MQ=60)
                                                                                     CGCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTT     <  SRR4302533.1135524/94‑1 (MQ=60)
                                                                                      GCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTG    <  SRR4302533.5790531/94‑1 (MQ=60)
                                                                                      GCGCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTG    <  SRR4302533.3432289/94‑1 (MQ=60)
                                                                                        GCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  SRR4302533.715319/1‑94 (MQ=60)
                                                                                        GCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  <  SRR4302533.464644/94‑1 (MQ=60)
                                                                                        GCTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGGTATGTCAACAAGCACAACGTTTCTCATTGAG  >  SRR4302533.1959377/1‑94 (MQ=60)
                                                                                        |                                                                                             
CCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAG  >  NC_000913/3850918‑3851099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑