Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,849,668 G→A G248G (GGC→GGT)  ilvB ← acetolactate synthase I large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,849,6680GA100.0% 816.6 / NA 229G248G (GGC→GGT) ilvBacetolactate synthase I large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (132/97);  total (132/97)

Only 100 of 232 total aligned reads displayed.

CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757
                                                                                          |                                                                                         
  aaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCa                                                                                       >  1:3232580/1‑93 (MQ=255)
  aaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCa                                                                                       <  2:4163108/93‑1 (MQ=255)
       gCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGcc                                                                                  >  2:408125/1‑93 (MQ=255)
       gCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGcc                                                                                  >  1:6068056/1‑93 (MQ=255)
         ctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAt                                                                                >  1:3907115/1‑93 (MQ=255)
         ctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAt                                                                                <  2:6090947/93‑1 (MQ=255)
          tcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAtt                                                                               >  2:24471/1‑93 (MQ=255)
          tcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAtt                                                                               >  2:714900/1‑93 (MQ=255)
           cctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTa                                                                              >  1:3923316/1‑93 (MQ=255)
           cctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTa                                                                              >  2:4013917/1‑93 (MQ=255)
            ctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaa                                                                             >  1:4678437/1‑93 (MQ=255)
            ctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaa                                                                             <  2:1533658/93‑1 (MQ=255)
            ctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaa                                                                             >  1:1209005/1‑93 (MQ=255)
                aaaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTc                                                                         <  1:4315244/93‑1 (MQ=255)
                aaaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTc                                                                         >  2:5852707/1‑93 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        >  2:1973135/1‑93 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        >  1:3825440/1‑93 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      >  1:5816038/1‑93 (MQ=255)
                     ataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggt                                                                    <  2:1393101/93‑1 (MQ=255)
                     ataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggt                                                                    >  2:5317645/1‑93 (MQ=255)
                     ataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggt                                                                    >  1:5731070/1‑93 (MQ=255)
                      taGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggtg                                                                   >  1:5166260/1‑93 (MQ=255)
                      taGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggtg                                                                   <  2:5768316/93‑1 (MQ=255)
                         ttGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTa                                                                <  2:3013004/93‑1 (MQ=255)
                         ttGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTa                                                                <  2:2791321/93‑1 (MQ=255)
                           ggTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAgg                                                              >  2:4886390/1‑93 (MQ=255)
                           ggTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAgg                                                              <  2:6041208/93‑1 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAg                                                           >  1:3497083/1‑93 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAg                                                           <  1:2181863/93‑1 (MQ=255)
                               ctgcGCACACCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGt                                                          >  2:6150726/1‑93 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         >  2:3116705/1‑93 (MQ=255)
                                cgcgcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         <  2:3170627/92‑1 (MQ=255)
                                 gcgcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTg                                                        >  2:1569618/1‑93 (MQ=255)
                                 gcgcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTg                                                        <  2:1755736/93‑1 (MQ=255)
                                  cgcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgc                                                       <  1:1022474/93‑1 (MQ=255)
                                    cACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgcgc                                                     >  1:2425009/1‑93 (MQ=255)
                                    cACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgcgc                                                     >  1:5329108/1‑93 (MQ=255)
                                    cACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgcgc                                                     <  1:3099639/93‑1 (MQ=255)
                                     aCGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCt                                                    >  1:4794685/1‑93 (MQ=255)
                                      cGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCtt                                                   >  1:6040558/1‑93 (MQ=255)
                                      cGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCtt                                                   <  1:2289700/93‑1 (MQ=255)
                                       gCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCttt                                                  >  2:4842629/1‑93 (MQ=255)
                                       gCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCttt                                                  >  1:1476824/1‑93 (MQ=255)
                                       gCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCttt                                                  >  2:1594908/1‑93 (MQ=255)
                                        ccGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtc                                                 >  2:5514901/1‑93 (MQ=255)
                                         cGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtct                                                >  2:503831/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               <  2:4260049/93‑1 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  1:2771058/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               <  1:620435/93‑1 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  1:5634359/1‑93 (MQ=255)
                                           tGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTcc                                              <  2:4114181/93‑1 (MQ=255)
                                            gCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCg                                             >  1:1082973/1‑93 (MQ=255)
                                              aTCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGcc                                           >  1:2011105/1‑93 (MQ=255)
                                               tCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCa                                          >  1:5048047/1‑93 (MQ=255)
                                                 cccAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTGCAGTCATGGTGGTAGGCAGTTGCGCTTTGTCCGCCAGt                                        >  1:851982/1‑93 (MQ=255)
                                                  ccAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGtt                                       >  2:3770601/1‑93 (MQ=255)
                                                   cAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTc                                      >  2:5150205/1‑93 (MQ=255)
                                                   cAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTc                                      >  1:3950180/1‑93 (MQ=255)
                                                   cAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTc                                      <  2:241324/93‑1 (MQ=255)
                                                    aGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCa                                     <  2:1096762/93‑1 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    >  2:5953793/1‑93 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    <  2:4490246/93‑1 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    <  2:875042/93‑1 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    <  2:2251475/93‑1 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  2:846967/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  1:4899289/1‑93 (MQ=255)
                                                          cccAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAcc                               <  1:662704/93‑1 (MQ=255)
                                                           ccAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAccc                              >  1:3334051/1‑93 (MQ=255)
                                                            cAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCg                             <  1:674325/93‑1 (MQ=255)
                                                             aGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGt                            <  1:1345961/93‑1 (MQ=255)
                                                              gCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTg                           <  2:4033729/93‑1 (MQ=255)
                                                               cGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGc                          <  1:1417272/93‑1 (MQ=255)
                                                               cGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGc                          <  1:828445/93‑1 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         >  2:1695273/1‑93 (MQ=255)
                                                                  cAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCggg                       >  2:3840901/1‑93 (MQ=255)
                                                                    aCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgcg                     >  1:2516928/1‑93 (MQ=255)
                                                                      ggATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCa                   >  1:3260223/1‑93 (MQ=255)
                                                                      cgATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCa                   <  1:1828585/92‑1 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  <  1:5853433/93‑1 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  <  2:3621978/93‑1 (MQ=255)
                                                                        aTGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAtt                 <  2:2293354/93‑1 (MQ=255)
                                                                          gcgcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGa               >  1:3571224/1‑93 (MQ=255)
                                                                          gcgcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGa               >  2:5585014/1‑93 (MQ=255)
                                                                          gcgcTTTGGGCAACATACCCAGCGCAATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGa               <  2:3662234/93‑1 (MQ=255)
                                                                            gcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATc             >  1:1537354/1‑93 (MQ=255)
                                                                              ttttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATcac           >  1:1603670/1‑93 (MQ=255)
                                                                               tttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATcaca          <  1:4768793/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         >  2:5480009/1‑93 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         >  1:295107/1‑93 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         <  1:3070149/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         >  2:2031742/1‑93 (MQ=255)
                                                                                 tGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAcc        >  1:263721/1‑93 (MQ=255)
                                                                                 tGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAcc        >  1:1001882/1‑93 (MQ=255)
                                                                                  ggCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg       >  1:820025/1‑93 (MQ=255)
                                                                                  ggCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg       >  2:1025248/1‑93 (MQ=255)
                                                                                   gCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgc      >  1:4621426/1‑93 (MQ=255)
                                                                                    cAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgcc     <  2:3458325/93‑1 (MQ=255)
                                                                                     aaCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgccg    >  2:5358803/1‑93 (MQ=255)
                                                                                     aaCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgccg    >  2:1225805/1‑93 (MQ=255)
                                                                                      aCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACcgccgc   >  2:3334018/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  >  NC_000913/3849575‑3849760
                                                                                             |                                                                                            
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATA                                                                                              >  SRR4302533.2623141/1‑94 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                             <  SRR4302533.88300/94‑1 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                             <  SRR4302533.2949024/94‑1 (MQ=60)
  ACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACC                                                                                            >  SRR4302533.3446202/1‑94 (MQ=60)
   CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCC                                                                                           <  SRR4302533.4021691/94‑1 (MQ=60)
   CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACACAGCGACAACGGATGCGCTTTTGGCAACATACCC                                                                                           >  SRR4302533.4168313/1‑94 (MQ=60)
    AAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCA                                                                                          <  SRR4302533.309559/94‑1 (MQ=60)
    AAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCA                                                                                          >  SRR4302533.3252498/1‑94 (MQ=60)
     AATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAACCAG                                                                                         >  SRR4302533.2769358/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                        >  SRR4302533.2991701/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                        <  SRR4302533.2476726/94‑1 (MQ=60)
       TCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCG                                                                                       <  SRR4302533.2302240/94‑1 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                     >  SRR4302533.6170034/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                     >  SRR4302533.6104773/1‑94 (MQ=60)
          GCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCA                                                                                    >  SRR4302533.5596330/1‑94 (MQ=60)
           CCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAT                                                                                   >  SRR4302533.3930979/1‑94 (MQ=60)
           CCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAT                                                                                   <  SRR4302533.3586483/94‑1 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                 >  SRR4302533.3684748/1‑94 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                 <  SRR4302533.1437166/94‑1 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                 >  SRR4302533.3947270/1‑94 (MQ=60)
              CCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAA                                                                                >  SRR4302533.1216266/1‑94 (MQ=60)
              CCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAA                                                                                >  SRR4302533.4706791/1‑94 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTCAA                                                                               >  SRR4302533.67189/1‑94 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAA                                                                               <  SRR4302533.5853590/94‑1 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAA                                                                               <  SRR4302533.5990315/94‑1 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                              >  SRR4302533.6077589/1‑94 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                              >  SRR4302533.1849075/1‑94 (MQ=60)
                   AAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCA                                                                           >  SRR4302533.3848826/1‑94 (MQ=60)
                   AAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCA                                                                           <  SRR4302533.4341227/94‑1 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                         >  SRR4302533.1419980/1‑94 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                         >  SRR4302533.5851165/1‑94 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                        <  SRR4302533.3934009/94‑1 (MQ=60)
                       TATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGT                                                                       >  SRR4302533.5731074/1‑94 (MQ=60)
                       TATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGT                                                                       >  SRR4302533.5765672/1‑94 (MQ=60)
                       TATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGG                                                                       >  SRR4302533.1173447/1‑94 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      >  SRR4302533.5197455/1‑94 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      >  SRR4302533.2588512/1‑94 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                     >  SRR4302533.2346004/1‑94 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                     <  SRR4302533.3127550/94‑1 (MQ=60)
                          AGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGT                                                                    >  SRR4302533.791329/1‑94 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                  <  SRR4302533.5863638/94‑1 (MQ=60)
                             TGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGG                                                                 <  SRR4302533.3812351/94‑1 (MQ=60)
                               GTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCA                                                               <  SRR4302533.3334655/94‑1 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                              >  SRR4302533.4909857/1‑94 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                              >  SRR4302533.3518647/1‑94 (MQ=60)
                                 GCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGT                                                             <  SRR4302533.638041/94‑1 (MQ=60)
                                 GCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGT                                                             <  SRR4302533.2195314/94‑1 (MQ=60)
                                  CTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTT                                                            <  SRR4302533.4955466/94‑1 (MQ=60)
                                     CGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCG                                                         <  SRR4302533.1028486/94‑1 (MQ=60)
                                     CGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCG                                                         <  SRR4302533.1257021/94‑1 (MQ=60)
                                      GCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGC                                                        >  SRR4302533.5361045/1‑94 (MQ=60)
                                      GCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGC                                                        >  SRR4302533.1536464/1‑94 (MQ=60)
                                      GCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGC                                                        >  SRR4302533.2439980/1‑94 (MQ=60)
                                       CACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCT                                                       <  SRR4302533.3118697/94‑1 (MQ=60)
                                       CACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCT                                                       >  SRR4302533.4823730/1‑94 (MQ=60)
                                       CACGCCGTGCAGCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCT                                                       <  SRR4302533.582675/94‑1 (MQ=60)
                                        ACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTT                                                      <  SRR4302533.436105/94‑1 (MQ=60)
                                        ACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTT                                                      >  SRR4302533.6077162/1‑94 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTT                                                     >  SRR4302533.1485751/1‑94 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTT                                                     <  SRR4302533.4678834/94‑1 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTT                                                     <  SRR4302533.2303878/94‑1 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                   <  SRR4302533.5612342/94‑1 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                  >  SRR4302533.5668429/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                  >  SRR4302533.2832008/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                  >  SRR4302533.2788058/1‑94 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                 <  SRR4302533.624079/94‑1 (MQ=60)
                                              TGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTCAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCC                                                >  SRR4302533.3927512/1‑94 (MQ=60)
                                              TGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCG                                                >  SRR4302533.1089360/1‑94 (MQ=60)
                                                CATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCC                                              >  SRR4302533.2023495/1‑94 (MQ=60)
                                                CATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCC                                              >  SRR4302533.1311973/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTTGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                             >  SRR4302533.2685385/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                             >  SRR4302533.3961697/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                             >  SRR4302533.5078583/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGGGGTAGGAAGTTGCGCTTTCTCCGCCA                                             >  SRR4302533.5190794/1‑94 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTGCAGTCATGGTGGTAGGCAGTTGCGCTTTGTCCGCCAGT                                           >  SRR4302533.856970/1‑94 (MQ=60)
                                                     CCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTC                                         >  SRR4302533.3974265/1‑94 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                       >  SRR4302533.1997303/1‑94 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACG                                      <  SRR4302533.1040475/94‑1 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                    >  SRR4302533.4928973/1‑94 (MQ=60)
                                                            ACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACC                                  <  SRR4302533.2328255/94‑1 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                 >  SRR4302533.3354651/1‑94 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                 <  SRR4302533.666618/94‑1 (MQ=60)
                                                              CCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCG                                <  SRR4302533.1534970/94‑1 (MQ=60)
                                                               CAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGT                               >  SRR4302533.326046/1‑94 (MQ=60)
                                                               CAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGT                               <  SRR4302533.678306/94‑1 (MQ=60)
                                                                AGCGACATCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                              <  SRR4302533.4776009/94‑1 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                              <  SRR4302533.4782052/94‑1 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                              <  SRR4302533.1354061/94‑1 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                              >  SRR4302533.1790181/1‑94 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                              >  SRR4302533.1570887/1‑94 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGTTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                              >  SRR4302533.6236536/1‑94 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                            <  SRR4302533.1425846/94‑1 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                            <  SRR4302533.833302/94‑1 (MQ=60)
                                                                     CAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGC                         <  SRR4302533.5239246/94‑1 (MQ=60)
                                                                      AACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCG                        <  SRR4302533.3292111/94‑1 (MQ=60)
                                                                      AACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCG                        >  SRR4302533.2532435/1‑94 (MQ=60)
                                                                        CGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCA                      >  SRR4302533.3280334/1‑94 (MQ=60)
                                                                         CGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                     <  SRR4302533.1839751/94‑1 (MQ=60)
                                                                          GATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATT                    <  SRR4302533.5888822/94‑1 (MQ=60)
                                                                           ATGGGGTTTTGGCAACATTCCCAGCGCCATTAAAGTCAAGGTGGTAAGCAATTGCGCCTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                   <  SRR4302533.2171749/94‑1 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                   >  SRR4302533.2035676/1‑94 (MQ=60)
                                                                            TGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGA                  <  SRR4302533.952170/94‑1 (MQ=60)
                                                                            TGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGA                  >  SRR4302533.3593232/1‑94 (MQ=60)
                                                                             GCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGAT                 <  SRR4302533.1012128/94‑1 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                >  SRR4302533.496376/1‑94 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                >  SRR4302533.1546589/1‑94 (MQ=60)
                                                                               GCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCA               <  SRR4302533.831838/94‑1 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC              >  SRR4302533.1613380/1‑94 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC              >  SRR4302533.3108276/1‑94 (MQ=60)
                                                                                  TTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAC            <  SRR4302533.4797697/94‑1 (MQ=60)
                                                                                  TTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAC            >  SRR4302533.296909/1‑94 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC           >  SRR4302533.1007732/1‑94 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC           >  SRR4302533.265321/1‑94 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC           <  SRR4302533.3088998/94‑1 (MQ=60)
                                                                                    TGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCG          >  SRR4302533.824833/1‑94 (MQ=60)
                                                                                     GGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGC         >  SRR4302533.4649435/1‑94 (MQ=60)
                                                                                     GGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGC         >  SRR4302533.4799509/1‑94 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG       >  SRR4302533.855929/1‑94 (MQ=60)
                                                                                        AACATACCCAGCGCCATTAAAGCCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGC      >  SRR4302533.762158/1‑94 (MQ=60)
                                                                                          CATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCC    >  SRR4302533.2466096/1‑94 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  <  SRR4302533.4037425/94‑1 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  <  SRR4302533.5270312/94‑1 (MQ=60)
                                                                                             |                                                                                            
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  >  NC_000913/3849575‑3849760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑