Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,883,371 T→C Y223H (TAC→CAC)  yidC → 60 KD inner‑membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,883,3710TC99.4% 1560.7 / ‑3.6 491Y223H (TAC→CAC) yidC60 KD inner‑membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (183/305);  total (184/307)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 512 total aligned reads displayed.

TTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGG  >  NC_000913/3883281‑3883460
                                                                                          |                                                                                         
tttGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCAc                                                                                         >  1:9522948/1‑93 (MQ=255)
  tGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTc                                                                                       <  2:144360/93‑1 (MQ=255)
   ggTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTcc                                                                                      >  2:2687038/1‑93 (MQ=255)
    gTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCa                                                                                     <  2:9688044/93‑1 (MQ=255)
    gTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCa                                                                                     <  2:3190647/93‑1 (MQ=255)
    gTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCa                                                                                     <  1:8651613/93‑1 (MQ=255)
     tCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCAc                                                                                    <  1:3531229/93‑1 (MQ=255)
       aGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGc                                                                                  <  2:5242836/93‑1 (MQ=255)
       aGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGc                                                                                  >  1:4611365/1‑93 (MQ=255)
        gTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGACGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGcc                                                                                 >  2:1760306/1‑93 (MQ=255)
        gTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGcc                                                                                 >  1:1673732/1‑93 (MQ=255)
         ttGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCt                                                                                >  2:5439745/1‑93 (MQ=255)
           gAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGa                                                                              <  1:2645171/93‑1 (MQ=255)
           gAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGa                                                                              >  2:9372812/1‑93 (MQ=255)
           gAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGa                                                                              <  2:9021897/93‑1 (MQ=255)
             aGCAATCCATCCCTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACg                                                                            <  2:2217213/93‑1 (MQ=255)
             aGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACATTCCGTGGCGCGACGCACTCCACGCCTGACg                                                                            >  2:1491395/1‑93 (MQ=255)
              gCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACga                                                                           <  1:1046516/93‑1 (MQ=255)
               cAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACgag                                                                          >  2:7123826/1‑93 (MQ=255)
                aaTCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACgaga                                                                         >  1:6832971/1‑93 (MQ=255)
                aaTCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACgaga                                                                         <  1:3723315/93‑1 (MQ=255)
                 aTCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGaa                                                                        <  1:199089/93‑1 (MQ=255)
                  tCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAg                                                                       >  1:4337251/1‑93 (MQ=255)
                  tCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAg                                                                       <  1:6388849/93‑1 (MQ=255)
                   ccATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGt                                                                      <  2:595923/93‑1 (MQ=255)
                   ccATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGt                                                                      <  2:3053159/93‑1 (MQ=255)
                   ccATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGt                                                                      <  2:8368849/93‑1 (MQ=255)
                    cATCCCTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTa                                                                     <  1:3041474/93‑1 (MQ=255)
                      tCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATg                                                                   <  2:2501212/93‑1 (MQ=255)
                       cACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATga                                                                  <  1:503060/93‑1 (MQ=255)
                       cACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATga                                                                  >  1:1191370/1‑93 (MQ=255)
                       cACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATga                                                                  >  1:2532018/1‑93 (MQ=255)
                        aCTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATgag                                                                 >  1:5976429/1‑93 (MQ=255)
                          tctGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGaa                                                               >  2:789415/1‑93 (MQ=255)
                           ctGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGaaa                                                              >  1:3945141/1‑93 (MQ=255)
                            tGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAAt                                                             >  2:7391272/1‑93 (MQ=255)
                             gCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATa                                                            >  2:3545936/1‑93 (MQ=255)
                             gCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATa                                                            <  1:2112495/93‑1 (MQ=255)
                              ccACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAAtgc                                                           >  1:4429454/1‑91 (MQ=255)
                                 ccGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAg                                                        >  2:9621591/1‑93 (MQ=255)
                                 ccGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAg                                                        >  1:4877889/1‑93 (MQ=255)
                                 ccGCATCTCGAGACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGGGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAg                                                        <  1:5096216/93‑1 (MQ=255)
                                    cATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTc                                                     <  1:1511136/93‑1 (MQ=255)
                                    cATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTc                                                     <  1:2795052/93‑1 (MQ=255)
                                     aTCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCg                                                    >  1:3174980/1‑93 (MQ=255)
                                      tctcGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGa                                                   >  2:4102576/1‑93 (MQ=255)
                                      actcGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGa                                                   <  1:7123582/92‑1 (MQ=255)
                                       ctcGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGAt                                                  <  1:5607855/93‑1 (MQ=255)
                                       ctcGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGAt                                                  <  2:8658641/93‑1 (MQ=255)
                                          gATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATAcc                                               <  1:2303882/93‑1 (MQ=255)
                                            tACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCAt                                             <  1:7695226/93‑1 (MQ=255)
                                             aCCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCAtt                                            >  1:9368316/1‑93 (MQ=255)
                                             aCCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCAtt                                            <  1:8065951/93‑1 (MQ=255)
                                               cGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGc                                          <  1:613429/93‑1 (MQ=255)
                                                ggAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGcc                                         <  2:6702695/93‑1 (MQ=255)
                                                 gAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCg                                        >  1:7256184/1‑93 (MQ=255)
                                                   agcagcAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAt                                      >  2:7611753/1‑93 (MQ=255)
                                                   agcagcAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAt                                      <  2:6544493/93‑1 (MQ=255)
                                                   agcagcAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAt                                      >  1:7822527/1‑93 (MQ=255)
                                                   agcagcAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACACCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAt                                      <  1:182883/93‑1 (MQ=255)
                                                    gcagcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATa                                     <  2:5607967/93‑1 (MQ=255)
                                                    gcagcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATa                                     <  2:3931308/93‑1 (MQ=255)
                                                     cagcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATaa                                    <  2:6863436/93‑1 (MQ=255)
                                                      agcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAAc                                   <  2:5075896/93‑1 (MQ=255)
                                                        caACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGa                                 <  2:1885525/93‑1 (MQ=255)
                                                         aaCTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGaa                                <  2:8127454/93‑1 (MQ=255)
                                                           cTTCGCACTGCACACCTTCCGTGGCGCGGCGCGCTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGaaaa                              <  2:3309296/93‑1 (MQ=255)
                                                           cTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGaaaa                              >  2:1219718/1‑93 (MQ=255)
                                                            ttCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAAc                             >  1:3347928/1‑93 (MQ=255)
                                                            ttCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAAc                             >  1:5742356/1‑93 (MQ=255)
                                                               gCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTg                          <  2:4225964/93‑1 (MQ=255)
                                                                cACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGa                         <  1:9208179/93‑1 (MQ=255)
                                                                  cTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAAc                       <  2:952634/93‑1 (MQ=255)
                                                                   tGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACa                      >  2:4810046/1‑93 (MQ=255)
                                                                    gCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAt                     >  1:5288833/1‑93 (MQ=255)
                                                                      acacCTGCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtct                   <  1:6993916/93‑1 (MQ=255)
                                                                       cacCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtctc                  <  1:2938013/93‑1 (MQ=255)
                                                                       cacCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtctc                  >  1:5416286/1‑93 (MQ=255)
                                                                        acCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATctct                 >  1:9716963/1‑93 (MQ=255)
                                                                        acCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATctct                 <  2:238171/93‑1 (MQ=255)
                                                                        acCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATctct                 <  1:6322829/93‑1 (MQ=255)
                                                                        acCTTCCGTGGCGCGGCGCACTCCACGCATGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATctct                 >  1:6612132/1‑93 (MQ=255)
                                                                         ccTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCtt                >  1:8304766/1‑93 (MQ=255)
                                                                         ccTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCtt                <  1:4178018/93‑1 (MQ=255)
                                                                         ccTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCtt                <  1:65205/93‑1 (MQ=255)
                                                                          cTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTc               <  1:9575922/93‑1 (MQ=255)
                                                                           ttCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCg              <  2:377912/93‑1 (MQ=255)
                                                                           ttCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCg              >  2:281265/1‑93 (MQ=255)
                                                                           ttCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCg              >  2:7703989/1‑93 (MQ=255)
                                                                              cGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaaa           <  1:6173712/93‑1 (MQ=255)
                                                                              cGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaaa           <  2:799065/93‑1 (MQ=255)
                                                                              cGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaaa           <  1:1396889/93‑1 (MQ=255)
                                                                               gTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATTTCTTCGAAAg          >  2:4401927/1‑93 (MQ=255)
                                                                               gTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAg          <  2:5351474/93‑1 (MQ=255)
                                                                                 ggcgcggcgcACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAggg        >  2:4327330/1‑92 (MQ=255)
                                                                                   cgcgGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAggtgg      <  1:9917238/93‑1 (MQ=255)
                                                                                    gcggcgCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAggtggt     <  2:3671248/93‑1 (MQ=255)
                                                                                     cggcgCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGACCATCTCTTCGAAAGGTGGtt    <  1:8867644/93‑1 (MQ=255)
                                                                                     cggcgCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGtt    <  2:8493743/93‑1 (MQ=255)
                                                                                      ggcgCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTg   >  1:6918176/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGG  >  NC_000913/3883281‑3883460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 290 total aligned reads displayed.
CGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGG  >  NC_000913/3883279‑3883464
                                                                                            |                                                                                             
CGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCA                                                                                              >  SRR4302540.2507113/1‑94 (MQ=60)
 GTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCAC                                                                                             >  SRR4302540.2359162/1‑94 (MQ=60)
 GTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCAC                                                                                             <  SRR4302540.1929750/94‑1 (MQ=60)
 GTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCAC                                                                                             >  SRR4302540.9635711/1‑94 (MQ=60)
 GTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCAC                                                                                             <  SRR4302540.3302106/94‑1 (MQ=60)
 GTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCAC                                                                                             <  SRR4302540.1534983/94‑1 (MQ=60)
 GTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCAC                                                                                             <  SRR4302540.7235084/94‑1 (MQ=60)
   TGGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTC                                                                                           <  SRR4302540.7789388/94‑1 (MQ=60)
     GGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCA                                                                                         >  SRR4302540.4465715/1‑94 (MQ=60)
     GGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCA                                                                                         >  SRR4302540.5058399/1‑94 (MQ=60)
     GGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCA                                                                                         <  SRR4302540.7874361/94‑1 (MQ=60)
      GTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCAC                                                                                        <  SRR4302540.8752116/94‑1 (MQ=60)
      GTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCAC                                                                                        <  SRR4302540.2664311/94‑1 (MQ=60)
       TCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACG                                                                                       >  SRR4302540.3664728/1‑94 (MQ=60)
        CAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGC                                                                                      >  SRR4302540.3010686/1‑94 (MQ=60)
        CAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGC                                                                                      >  SRR4302540.4660857/1‑94 (MQ=60)
         AGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCC                                                                                     >  SRR4302540.1691917/1‑94 (MQ=60)
           TTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTG                                                                                   <  SRR4302540.3190971/94‑1 (MQ=60)
           TTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTG                                                                                   <  SRR4302540.2152165/94‑1 (MQ=60)
           TTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTG                                                                                   <  SRR4302540.7208171/94‑1 (MQ=60)
           TTGAAGCAATCCATCACCCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTG                                                                                   <  SRR4302540.7785585/94‑1 (MQ=60)
            TGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGA                                                                                  >  SRR4302540.1142387/1‑94 (MQ=60)
             GAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGAC                                                                                 <  SRR4302540.2673868/94‑1 (MQ=60)
             GAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGAC                                                                                 <  SRR4302540.6541154/94‑1 (MQ=60)
             GAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGAC                                                                                 <  SRR4302540.4084277/94‑1 (MQ=60)
              AAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACG                                                                                <  SRR4302540.4557603/94‑1 (MQ=60)
                GCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCTGCGCACTCCACGCCTGACGAG                                                                              >  SRR4302540.440781/1‑94 (MQ=60)
                GCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAG                                                                              <  SRR4302540.6350638/94‑1 (MQ=60)
                GCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAG                                                                              <  SRR4302540.1851741/94‑1 (MQ=60)
                GCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAG                                                                              <  SRR4302540.1057151/94‑1 (MQ=60)
                GCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAG                                                                              <  SRR4302540.3260678/94‑1 (MQ=60)
                ACAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAG                                                                              <  SRR4302540.6350637/94‑1 (MQ=60)
                 CAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGA                                                                             >  SRR4302540.6908647/1‑94 (MQ=60)
                 CAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGA                                                                             <  SRR4302540.3518615/94‑1 (MQ=60)
                  AATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAA                                                                            >  SRR4302540.3788346/1‑94 (MQ=60)
                  AATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAA                                                                            <  SRR4302540.1315269/94‑1 (MQ=60)
                   ATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAG                                                                           >  SRR4302540.4384470/1‑94 (MQ=60)
                   ATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAG                                                                           <  SRR4302540.5333764/94‑1 (MQ=60)
                   ATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAG                                                                           <  SRR4302540.5300475/94‑1 (MQ=60)
                    TCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGT                                                                          <  SRR4302540.6459967/94‑1 (MQ=60)
                    GCCATCACTCTCCCACCGCAGCTCGATGCCGGAAGCAGCCACTTCGCACTGCACACCTGCCGGGGCGCGGCGCACTCCACGCCTGACGAGAAGT                                                                          <  SRR4302540.1015215/94‑1 (MQ=60)
                     CCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTA                                                                         <  SRR4302540.6928604/94‑1 (MQ=60)
                      CATCCCTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTAT                                                                        <  SRR4302540.3074851/94‑1 (MQ=60)
                      CATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTAT                                                                        <  SRR4302540.1672578/94‑1 (MQ=60)
                      CATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTAT                                                                        <  SRR4302540.9142182/94‑1 (MQ=60)
                      CATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTAT                                                                        <  SRR4302540.371129/94‑1 (MQ=60)
                       ATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATG                                                                       <  SRR4302540.10091317/94‑1 (MQ=60)
                        TCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGA                                                                      >  SRR4302540.1203650/1‑94 (MQ=60)
                        TCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGA                                                                      >  SRR4302540.2559336/1‑94 (MQ=60)
                         CACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAG                                                                     >  SRR4302540.6042587/1‑94 (MQ=60)
                         CACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAG                                                                     >  SRR4302540.9856430/1‑94 (MQ=60)
                         CACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAG                                                                     >  SRR4302540.9008697/1‑94 (MQ=60)
                         CACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAG                                                                     >  SRR4302540.185098/1‑94 (MQ=60)
                         CACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAG                                                                     <  SRR4302540.508453/94‑1 (MQ=60)
                           CTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAA                                                                   >  SRR4302540.9600550/1‑94 (MQ=60)
                           CTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAA                                                                   <  SRR4302540.5444076/94‑1 (MQ=60)
                           CTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAA                                                                   <  SRR4302540.1165253/94‑1 (MQ=60)
                             CTGCCACCGCATCTCGATACCGGAAGCAGCACCTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAAT                                                                 <  SRR4302540.2727959/94‑1 (MQ=60)
                             CTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAAT                                                                 <  SRR4302540.467655/94‑1 (MQ=60)
                             CTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAAT                                                                 <  SRR4302540.6223504/94‑1 (MQ=60)
                             CTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAAT                                                                 <  SRR4302540.6223503/94‑1 (MQ=60)
                              TGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATA                                                                <  SRR4302540.9669358/94‑1 (MQ=60)
                              TGCCACCGCATCTCGATACAGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATA                                                                >  SRR4302540.7975856/1‑94 (MQ=60)
                               GCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGTGAAGTATGAGAAAGAC                                                               >  SRR4302540.5025202/1‑94 (MQ=60)
                               GCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATGC                                                               >  SRR4302540.4477448/1‑94 (MQ=60)
                               GCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATAC                                                               <  SRR4302540.4443315/94‑1 (MQ=60)
                               GCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATAC                                                               <  SRR4302540.2135168/94‑1 (MQ=60)
                                CCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACA                                                              >  SRR4302540.6658477/1‑94 (MQ=60)
                                 CACCGCATCTCGATGCCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAA                                                             <  SRR4302540.5709150/94‑1 (MQ=60)
                                  CCCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAG                                                            <  SRR4302540.6899001/94‑1 (MQ=60)
                                  CCCGCACCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCTGGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAG                                                            <  SRR4302540.3162217/94‑1 (MQ=60)
                                  ACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAG                                                            >  SRR4302540.4930257/1‑94 (MQ=60)
                                   CCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGT                                                           <  SRR4302540.8240527/94‑1 (MQ=60)
                                   CCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGT                                                           <  SRR4302540.4171972/94‑1 (MQ=60)
                                   CCGCATCTCGAGACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGGGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGT                                                           <  SRR4302540.5151360/94‑1 (MQ=60)
                                    CGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTT                                                          >  SRR4302540.9550168/1‑94 (MQ=60)
                                    CGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTT                                                          <  SRR4302540.706677/94‑1 (MQ=60)
                                    CGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTT                                                          <  SRR4302540.5373847/94‑1 (MQ=60)
                                     GCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTC                                                         <  SRR4302540.7008911/94‑1 (MQ=60)
                                     GCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTC                                                         <  SRR4302540.2398075/94‑1 (MQ=60)
                                      CATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCG                                                        <  SRR4302540.3703325/94‑1 (MQ=60)
                                      CATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCG                                                        <  SRR4302540.1527834/94‑1 (MQ=60)
                                      CATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCG                                                        <  SRR4302540.2825228/94‑1 (MQ=60)
                                       ATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGA                                                       >  SRR4302540.9331042/1‑94 (MQ=60)
                                        TCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGAT                                                      <  SRR4302540.7157224/94‑1 (MQ=60)
                                        TCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGAT                                                      <  SRR4302540.9701534/94‑1 (MQ=60)
                                        TCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGAT                                                      <  SRR4302540.5093956/94‑1 (MQ=60)
                                        ACTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGAT                                                      <  SRR4302540.7202910/94‑1 (MQ=60)
                                         CTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATA                                                     <  SRR4302540.5669553/94‑1 (MQ=60)
                                           CGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACC                                                   >  SRR4302540.2092577/1‑94 (MQ=60)
                                           CGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACC                                                   <  SRR4302540.6941809/94‑1 (MQ=60)
                                           CGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACC                                                   >  SRR4302540.9664408/1‑94 (MQ=60)
                                           CGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACC                                                   <  SRR4302540.8871853/94‑1 (MQ=60)
                                           CGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACC                                                   >  SRR4302540.8214838/1‑94 (MQ=60)
                                            GATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCA                                                  >  SRR4302540.3012389/1‑94 (MQ=60)
                                             ATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCAT                                                 <  SRR4302540.4341692/94‑1 (MQ=60)
                                             ATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCAT                                                 <  SRR4302540.8657921/94‑1 (MQ=60)
                                             ATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCAT                                                 <  SRR4302540.9635686/94‑1 (MQ=60)
                                              TACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATT                                                <  SRR4302540.3854539/94‑1 (MQ=60)
                                              TACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATT                                                <  SRR4302540.7782886/94‑1 (MQ=60)
                                              TACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATT                                                <  SRR4302540.210918/94‑1 (MQ=60)
                                              TACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATT                                                <  SRR4302540.6403783/94‑1 (MQ=60)
                                               ACCGGAAGCAGCAACTTCGCACTGCACTCCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTG                                               <  SRR4302540.7722456/94‑1 (MQ=60)
                                               ACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTG                                               <  SRR4302540.7722457/94‑1 (MQ=60)
                                               ACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTG                                               <  SRR4302540.1439316/94‑1 (MQ=60)
                                               ACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTG                                               <  SRR4302540.5641339/94‑1 (MQ=60)
                                               ACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTG                                               <  SRR4302540.8159555/94‑1 (MQ=60)
                                                  GGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCG                                            >  SRR4302540.7337151/1‑94 (MQ=60)
                                                  GGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCG                                            <  SRR4302540.9443082/94‑1 (MQ=60)
                                                    AAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCcaa                                          >  SRR4302540.2209343/1‑91 (MQ=60)
                                                    AAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAT                                          >  SRR4302540.5869109/1‑94 (MQ=60)
                                                    AAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAT                                          <  SRR4302540.6845061/94‑1 (MQ=60)
                                                    AAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAT                                          <  SRR4302540.2080842/94‑1 (MQ=60)
                                                    AAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAT                                          <  SRR4302540.4914236/94‑1 (MQ=60)
                                                    AAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAT                                          >  SRR4302540.7913941/1‑94 (MQ=60)
                                                    AAGCAGCAACTCCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGAT                                          <  SRR4302540.6605112/94‑1 (MQ=60)
                                                     AGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATA                                         <  SRR4302540.7154405/94‑1 (MQ=60)
                                                     AGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATA                                         <  SRR4302540.6429160/94‑1 (MQ=60)
                                                     AGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATA                                         <  SRR4302540.1903437/94‑1 (MQ=60)
                                                       CAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAAC                                       <  SRR4302540.1790673/94‑1 (MQ=60)
                                                       CAGCAACTTCGCACAGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAAC                                       <  SRR4302540.6440927/94‑1 (MQ=60)
                                                        AGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACG                                      <  SRR4302540.5748988/94‑1 (MQ=60)
                                                        AGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACG                                      <  SRR4302540.6549979/94‑1 (MQ=60)
                                                        AGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACG                                      <  SRR4302540.279816/94‑1 (MQ=60)
                                                         GCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGA                                     >  SRR4302540.10000890/1‑94 (MQ=60)
                                                         GCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGA                                     >  SRR4302540.4023277/1‑94 (MQ=60)
                                                         GCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGA                                     >  SRR4302540.4501165/1‑94 (MQ=60)
                                                         GCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGAATGAGAAATACAAGTTCGATACCCTTGCCGATAAAGA                                     >  SRR4302540.3412868/1‑94 (MQ=60)
                                                           AACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAA                                   >  SRR4302540.6322960/1‑94 (MQ=60)
                                                           AACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAA                                   <  SRR4302540.288837/94‑1 (MQ=60)
                                                            ACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAA                                  >  SRR4302540.9017720/1‑94 (MQ=60)
                                                            ACTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAA                                  >  SRR4302540.9513681/1‑94 (MQ=60)
                                                             CTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAAC                                 >  SRR4302540.5805659/1‑94 (MQ=60)
                                                             CTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAAC                                 >  SRR4302540.3384642/1‑94 (MQ=60)
                                                             CTTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAAC                                 >  SRR4302540.6174889/1‑94 (MQ=60)
                                                              TTCGCACTGCACACCTTCCGTGGCGCGGCGCGCTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACC                                <  SRR4302540.8627648/94‑1 (MQ=60)
                                                              TTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACC                                <  SRR4302540.946372/94‑1 (MQ=60)
                                                              TTCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACC                                <  SRR4302540.2594008/94‑1 (MQ=60)
                                                               TCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACNAAAACCT                               <  SRR4302540.666592/94‑1 (MQ=60)
                                                               TCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCT                               <  SRR4302540.9938776/94‑1 (MQ=60)
                                                               TCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCT                               <  SRR4302540.6049188/94‑1 (MQ=60)
                                                               TCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCT                               <  SRR4302540.6206728/94‑1 (MQ=60)
                                                               TCGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCT                               <  SRR4302540.3398793/94‑1 (MQ=60)
                                                                CGCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTG                              <  SRR4302540.7484112/94‑1 (MQ=60)
                                                                 GCACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGA                             <  SRR4302540.6394345/94‑1 (MQ=60)
                                                                  CACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAA                            <  SRR4302540.9318671/94‑1 (MQ=60)
                                                                  CACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGACGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAA                            >  SRR4302540.5893824/1‑94 (MQ=60)
                                                                   CCTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAAC                           <  SRR4302540.2958130/94‑1 (MQ=60)
                                                                   ACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAAC                           >  SRR4302540.877691/1‑94 (MQ=60)
                                                                   ACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAAC                           >  SRR4302540.1297309/1‑94 (MQ=60)
                                                                   ACTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAAC                           <  SRR4302540.4679662/94‑1 (MQ=60)
                                                                    CTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACA                          >  SRR4302540.1205559/1‑94 (MQ=60)
                                                                    CTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACA                          <  SRR4302540.3215479/94‑1 (MQ=60)
                                                                    CTGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACA                          >  SRR4302540.9768291/1‑94 (MQ=60)
                                                                     TGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAT                         >  SRR4302540.1563731/1‑94 (MQ=60)
                                                                     TGCACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAT                         >  SRR4302540.5346638/1‑94 (MQ=60)
                                                                       CACACCTTCCGTGGCGCTGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCT                       >  SRR4302540.9773933/1‑94 (MQ=60)
                                                                        ACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTC                      <  SRR4302540.7071541/94‑1 (MQ=60)
                                                                        ACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTC                      >  SRR4302540.5475844/1‑94 (MQ=60)
                                                                        ACACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTC                      <  SRR4302540.7966730/94‑1 (MQ=60)
                                                                         CACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCT                     >  SRR4302540.5572387/1‑94 (MQ=60)
                                                                         CACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCT                     >  SRR4302540.3982415/1‑94 (MQ=60)
                                                                         CACCTTCCGTGGCGCGGCGCACTCCACGCATGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCT                     >  SRR4302540.6685348/1‑94 (MQ=60)
                                                                          ACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTT                    >  SRR4302540.8368575/1‑94 (MQ=60)
                                                                          ACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTT                    >  SRR4302540.8401201/1‑94 (MQ=60)
                                                                          ACCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTT                    <  SRR4302540.3419122/94‑1 (MQ=60)
                                                                           CCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTC                   <  SRR4302540.4223378/94‑1 (MQ=60)
                                                                           CCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTC                   <  SRR4302540.65862/94‑1 (MQ=60)
                                                                           CCTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTC                   >  SRR4302540.251553/1‑94 (MQ=60)
                                                                            CTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCG                  <  SRR4302540.9689021/94‑1 (MQ=60)
                                                                            CTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCG                  <  SRR4302540.5131632/94‑1 (MQ=60)
                                                                            CTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCG                  >  SRR4302540.5141391/1‑94 (MQ=60)
                                                                            CTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCG                  <  SRR4302540.7056373/94‑1 (MQ=60)
                                                                            CTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCG                  >  SRR4302540.2567326/1‑94 (MQ=60)
                                                                            CTTCCGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCG                  <  SRR4302540.6895617/94‑1 (MQ=60)
                                                                                CGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAG              <  SRR4302540.1411880/94‑1 (MQ=60)
                                                                                CGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAG              <  SRR4302540.3571561/94‑1 (MQ=60)
                                                                                CGTGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAG              <  SRR4302540.6242501/94‑1 (MQ=60)
                                                                                  TGGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGT            <  SRR4302540.4319531/94‑1 (MQ=60)
                                                                                   GGCGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATCCCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTG           <  SRR4302540.5467216/94‑1 (MQ=60)
                                                                                     CGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGT         <  SRR4302540.10033056/94‑1 (MQ=60)
                                                                                     CGCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGT         <  SRR4302540.6139157/94‑1 (MQ=60)
                                                                                      GCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTT        <  SRR4302540.2915039/94‑1 (MQ=60)
                                                                                      GCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTT        <  SRR4302540.5689740/94‑1 (MQ=60)
                                                                                      GCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTT        <  SRR4302540.7255558/94‑1 (MQ=60)
                                                                                      GCGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTT        <  SRR4302540.1783547/94‑1 (MQ=60)
                                                                                      GCCGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTT        <  SRR4302540.3095182/94‑1 (MQ=60)
                                                                                       CGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTG       >  SRR4302540.6995157/1‑94 (MQ=60)
                                                                                       CGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTG       <  SRR4302540.227201/94‑1 (MQ=60)
                                                                                       CGGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTG       <  SRR4302540.8818603/94‑1 (MQ=60)
                                                                                        GGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGG      <  SRR4302540.1836473/94‑1 (MQ=60)
                                                                                        GGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGG      <  SRR4302540.1442404/94‑1 (MQ=60)
                                                                                        GGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGG      <  SRR4302540.5162691/94‑1 (MQ=60)
                                                                                        GGCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGG      >  SRR4302540.3007054/1‑94 (MQ=60)
                                                                                         GCGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGG     >  SRR4302540.7878381/1‑94 (MQ=60)
                                                                                          CGCACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGT    <  SRR4302540.7855163/94‑1 (MQ=60)
                                                                                            CACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGG  >  SRR4302540.1610820/1‑94 (MQ=60)
                                                                                            CACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGG  <  SRR4302540.3960571/94‑1 (MQ=60)
                                                                                            CACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGG  >  SRR4302540.3808942/1‑94 (MQ=60)
                                                                                            CACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGG  >  SRR4302540.3216328/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGG  >  NC_000913/3883279‑3883464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑