Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,893,993 A→G A82A (GCT→GCC)  yieG ← putative membrane/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,893,9930AG99.8% 1480.1 / ‑5.3 452A82A (GCT→GCC) yieGputative membrane/transport protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (231/220);  total (232/220)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

Only 100 of 471 total aligned reads displayed.

GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAG  >  NC_000913/3893903‑3894082
                                                                                          |                                                                                         
gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGc                                                                                         >  1:5622705/1‑93 (MQ=255)
 aCCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCa                                                                                        <  1:3388988/93‑1 (MQ=255)
   cTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCaaa                                                                                      >  1:8522383/1‑93 (MQ=255)
   cTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCaaa                                                                                      >  1:2844328/1‑93 (MQ=255)
   cTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCaaa                                                                                      >  1:2614168/1‑93 (MQ=255)
    tGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAAc                                                                                     >  2:1086872/1‑93 (MQ=255)
      ccACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAg                                                                                   >  2:4717132/1‑93 (MQ=255)
      ccACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAg                                                                                   <  2:9199330/93‑1 (MQ=255)
        aCGGCAAGCCCATCGCTTGTACAATGACAGAAGCGAAGAACGCATTCAGGCCCATAGCGTGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTc                                                                                 <  1:5577034/93‑1 (MQ=255)
        aCGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTc                                                                                 <  2:6465636/93‑1 (MQ=255)
        aCGGCAAGCACATCGCCTGTACAAGGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTc                                                                                 <  1:4871926/93‑1 (MQ=255)
          gtcAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTccc                                                                               >  1:7973347/3‑93 (MQ=255)
           gCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCca                                                                              >  1:7102049/1‑93 (MQ=255)
             aaGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatc                                                                            <  1:5767159/93‑1 (MQ=255)
              aGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACCGTCCcatca                                                                           >  2:2584398/1‑93 (MQ=255)
              aGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatca                                                                           >  2:5670686/1‑93 (MQ=255)
              aGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatca                                                                           <  1:3594729/93‑1 (MQ=255)
               ccccATCGCCTGAACAACAAAAAAAGCAAAGAACGCATTCAGGCCCATGGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatcat                                                                          <  2:6599336/92‑1 (MQ=255)
                 ccATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataa                                                                        >  1:4701415/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                       >  2:4399845/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                       >  2:3310700/1‑93 (MQ=255)
                   aTCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataata                                                                      >  1:10009714/1‑93 (MQ=255)
                     cGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACt                                                                    <  2:3232193/93‑1 (MQ=255)
                      gCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTg                                                                   >  2:2405911/1‑93 (MQ=255)
                        cTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGcc                                                                 <  1:2544731/93‑1 (MQ=255)
                        cTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGcc                                                                 <  2:3536848/93‑1 (MQ=255)
                         tGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCg                                                                >  1:965718/1‑93 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                              <  1:4356137/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                             <  2:377451/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                             >  2:4091853/1‑93 (MQ=255)
                             cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATg                                                            <  1:7745333/93‑1 (MQ=255)
                               aCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCa                                                          <  1:1670806/93‑1 (MQ=255)
                               aCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGACAAACAGTCCCATCATAATACTGCCGAATGCa                                                          <  1:5937765/93‑1 (MQ=255)
                                cGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAACCAGTCCCATCATAATACTGCCGAATGCAg                                                         <  2:2105928/93‑1 (MQ=255)
                                 gACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGc                                                        <  1:3734894/93‑1 (MQ=255)
                                   cAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGa                                                      >  2:5260286/1‑93 (MQ=255)
                                    aaaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACATTCCCATCATAATACTGCCGAATGCAGCGAt                                                     <  1:8862014/93‑1 (MQ=255)
                                    aaaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAt                                                     <  1:657460/93‑1 (MQ=255)
                                    aaaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAt                                                     >  1:3081258/1‑93 (MQ=255)
                                    aaaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAt                                                     <  2:7204746/93‑1 (MQ=255)
                                    aaaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAt                                                     >  2:6148917/1‑93 (MQ=255)
                                       aGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAg                                                  <  2:2302769/93‑1 (MQ=255)
                                        gCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGa                                                 <  2:7032763/93‑1 (MQ=255)
                                        gCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGa                                                 <  2:8549177/93‑1 (MQ=255)
                                        gCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGa                                                 <  1:205735/93‑1 (MQ=255)
                                        aagaagaaCGCATTCAGGCCAATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGa                                                 <  2:7402028/91‑1 (MQ=255)
                                          gaagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACa                                               >  1:9438977/1‑93 (MQ=255)
                                          gaagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACa                                               <  2:3103619/93‑1 (MQ=255)
                                              aaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                           <  2:9278942/93‑1 (MQ=255)
                                               aCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAg                                          <  2:5859872/93‑1 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       <  2:1643116/93‑1 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       >  1:1503075/1‑93 (MQ=255)
                                                   aTTCAGGCGCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAc                                      <  2:7605543/93‑1 (MQ=255)
                                                   aTTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAc                                      >  2:5099054/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                     >  1:4279655/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                     >  2:1981514/1‑93 (MQ=255)
                                                     tCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGa                                    >  1:9171179/1‑93 (MQ=255)
                                                     tCAGGCCCATAGCGGGTGCCAGTGCAACTGGAAGGTGGGAAAAAAGTCCCATCATAAACCTGCCGAATGCAGGGATCAGCCAGGAGGGGACGa                                    >  1:9314493/1‑93 (MQ=255)
                                                       aGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAg                                  <  2:2984663/93‑1 (MQ=255)
                                                       aGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAg                                  <  1:7397014/93‑1 (MQ=255)
                                                       aGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAg                                  <  1:6531089/93‑1 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 <  1:4137347/93‑1 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 <  2:6886057/93‑1 (MQ=255)
                                                         gCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAc                                <  1:4336462/93‑1 (MQ=255)
                                                          cccATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACg                               <  1:2138871/93‑1 (MQ=255)
                                                           ccATAGCAGGTGCCAGTGCAGCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCGGAATGCAGCGATCAGACAGGTAGTGACGAAGACgt                              <  2:1929891/93‑2 (MQ=255)
                                                            cATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgtc                             >  1:632141/1‑91 (MQ=255)
                                                              tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                           >  1:2373088/1‑93 (MQ=255)
                                                              tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                           >  1:3040283/1‑93 (MQ=255)
                                                                gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                         >  2:5355510/1‑93 (MQ=255)
                                                                  gggTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGt                       >  1:7194245/1‑93 (MQ=255)
                                                                  gggTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGt                       <  1:5253332/93‑1 (MQ=255)
                                                                   ggTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTa                      >  2:579669/1‑93 (MQ=255)
                                                                    gTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAt                     >  2:1435540/1‑93 (MQ=255)
                                                                    gTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAt                     >  1:110175/1‑93 (MQ=255)
                                                                     tGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATc                    >  1:9552421/1‑93 (MQ=255)
                                                                     tGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATc                    <  1:1894225/93‑1 (MQ=255)
                                                                       ccAGTGCAACTGTCAGGTTGGCAAACAGCCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                  <  2:8065407/93‑1 (MQ=255)
                                                                       ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                  <  2:6001300/93‑1 (MQ=255)
                                                                        cAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAt                 >  1:8179658/1‑93 (MQ=255)
                                                                          gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTCTCCATGc               >  1:6744614/1‑93 (MQ=255)
                                                                          gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGc               <  1:6881305/93‑1 (MQ=255)
                                                                           tGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGcc              >  2:6355212/1‑93 (MQ=255)
                                                                            gCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCa             >  1:9820827/1‑93 (MQ=255)
                                                                            gCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCa             <  2:9093337/93‑1 (MQ=255)
                                                                             cagcTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAg            <  1:4569886/90‑1 (MQ=255)
                                                                             cAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAg            <  1:1320961/93‑1 (MQ=255)
                                                                               aCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCa          >  2:9604582/1‑93 (MQ=255)
                                                                               aCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCa          >  1:7907408/1‑93 (MQ=255)
                                                                                 tGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAc        >  1:251939/1‑93 (MQ=255)
                                                                                   gCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGc      <  2:9779159/93‑1 (MQ=255)
                                                                                    cAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGcc     >  2:5556445/1‑93 (MQ=255)
                                                                                     aGGTTGGCAAACAGTCCCATCATAATGCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa    <  1:4972322/93‑1 (MQ=255)
                                                                                     aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa    >  2:270803/1‑93 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa   >  2:7867963/1‑93 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa   <  1:6268077/93‑1 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa   >  1:838782/1‑93 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa   >  2:10016234/1‑93 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa   >  2:4810069/1‑93 (MQ=255)
                                                                                       gTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAg  >  2:881091/1‑93 (MQ=255)
                                                                                          |                                                                                         
GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAG  >  NC_000913/3893903‑3894082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 261 total aligned reads displayed.
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  NC_000913/3893900‑3894086
                                                                                             |                                                                                             
CCCGCCCTGCCACGGCAAGTCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               <  SRR4302540.6947086/94‑1 (MQ=60)
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               <  SRR4302540.1177696/94‑1 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                             >  SRR4302540.5684574/1‑94 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                             >  SRR4302540.8102558/1‑94 (MQ=60)
   GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCA                                                                                            >  SRR4302540.4551662/1‑94 (MQ=60)
    ACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAA                                                                                           >  SRR4302540.2618201/1‑94 (MQ=60)
    ACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAA                                                                                           <  SRR4302540.3425967/94‑1 (MQ=60)
     CCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAA                                                                                          >  SRR4302540.2875107/1‑94 (MQ=60)
     CCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAA                                                                                          >  SRR4302540.8621421/1‑94 (MQ=60)
      CTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAAC                                                                                         <  SRR4302540.9445623/94‑1 (MQ=60)
      CTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGCGCCAGTGCAACTGGCAGGTTGGCAAAC                                                                                         >  SRR4302540.3681749/1‑94 (MQ=60)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                        >  SRR4302540.7530485/1‑94 (MQ=60)
       TGCCACGGCAAGCCCATCGCCGGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                        <  SRR4302540.9378707/94‑1 (MQ=60)
       CGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                        <  SRR4302540.1365335/94‑1 (MQ=60)
         CCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGT                                                                                      >  SRR4302540.880598/1‑94 (MQ=60)
         CCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGT                                                                                      >  SRR4302540.503537/1‑94 (MQ=60)
         CCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGT                                                                                      >  SRR4302540.7246110/1‑94 (MQ=60)
         CCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGT                                                                                      <  SRR4302540.7239400/94‑1 (MQ=60)
          CACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTC                                                                                     <  SRR4302540.6942436/94‑1 (MQ=60)
          CACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTC                                                                                     >  SRR4302540.9451070/1‑94 (MQ=60)
           ACGGCAAGCCCATCGCTTGTACAATGACAGAAGCGAAGAACGCATTCAGGCCCATAGCGTGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCC                                                                                    <  SRR4302540.5638434/94‑1 (MQ=60)
           ACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCC                                                                                    <  SRR4302540.6621218/94‑1 (MQ=60)
            CGTCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCC                                                                                   >  SRR4302540.8066244/1‑94 (MQ=60)
            CGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCC                                                                                   <  SRR4302540.4815173/94‑1 (MQ=60)
               CAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATC                                                                                <  SRR4302540.8540112/94‑1 (MQ=60)
                AAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCA                                                                               <  SRR4302540.5830844/94‑1 (MQ=60)
                AAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCA                                                                               >  SRR4302540.1632733/1‑94 (MQ=60)
                AAGCCCATCGCCTGGACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCA                                                                               >  SRR4302540.6793508/1‑94 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                              >  SRR4302540.1029750/1‑94 (MQ=60)
                  GCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATA                                                                             >  SRR4302540.9416312/1‑94 (MQ=60)
                   CCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAA                                                                            >  SRR4302540.2591881/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           >  SRR4302540.8318695/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           <  SRR4302540.1515907/94‑1 (MQ=60)
                     CATCGCCTGTACAACGACACAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATA                                                                          >  SRR4302540.3974312/1‑94 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAC                                                                         >  SRR4302540.4837977/1‑94 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAC                                                                         <  SRR4302540.3588176/94‑1 (MQ=60)
                       TCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACT                                                                        <  SRR4302540.3494235/94‑1 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTG                                                                       >  SRR4302540.8761180/1‑94 (MQ=60)
                         GCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGC                                                                      <  SRR4302540.4202517/94‑1 (MQ=60)
                         GCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGC                                                                      >  SRR4302540.561864/1‑94 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCTGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     <  SRR4302540.37929/94‑1 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     <  SRR4302540.7378695/94‑1 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                    <  SRR4302540.2341323/94‑1 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                    <  SRR4302540.517662/94‑1 (MQ=60)
                            TGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGA                                                                   >  SRR4302540.4939522/1‑94 (MQ=60)
                             GTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAA                                                                  >  SRR4302540.5786142/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 >  SRR4302540.2416324/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 >  SRR4302540.1131889/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302540.4403518/94‑1 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302540.9220626/94‑1 (MQ=60)
                              GACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302540.2692849/94‑1 (MQ=60)
                                CAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGC                                                               >  SRR4302540.2432199/1‑94 (MQ=60)
                                CAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGC                                                               >  SRR4302540.9407750/1‑94 (MQ=60)
                                CAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGC                                                               <  SRR4302540.7835769/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              <  SRR4302540.3919568/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              <  SRR4302540.3068428/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              >  SRR4302540.3241826/1‑94 (MQ=60)
                                  ccgccaaatgcgacGAACGCATTCAGGCCCATAGCCGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAG                                                             <  SRR4302540.988522/80‑1 (MQ=60)
                                  ACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAG                                                             <  SRR4302540.4715810/94‑1 (MQ=60)
                                  ACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAG                                                             <  SRR4302540.1688977/94‑1 (MQ=60)
                                  ACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAG                                                             <  SRR4302540.4715811/94‑1 (MQ=60)
                                  ACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGACAAACAGTCCCATCATAATACTGCCGAATGCAG                                                             <  SRR4302540.6003403/94‑1 (MQ=60)
                                   CGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGC                                                            >  SRR4302540.694444/1‑94 (MQ=60)
                                   CGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGC                                                            <  SRR4302540.4910672/94‑1 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302540.9628230/94‑1 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           >  SRR4302540.9872164/1‑94 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           >  SRR4302540.2946202/1‑94 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302540.2901802/94‑1 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302540.3775644/94‑1 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302540.2643013/94‑1 (MQ=60)
                                      CAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAT                                                         >  SRR4302540.3115071/1‑94 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACATTCCCATCATAATACTGCCGAATGCAGCGATC                                                        <  SRR4302540.8964562/94‑1 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        <  SRR4302540.6609100/94‑1 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        <  SRR4302540.6609099/94‑1 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        <  SRR4302540.664473/94‑1 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        <  SRR4302540.1180339/94‑1 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        >  SRR4302540.9856344/1‑94 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        >  SRR4302540.4389576/1‑94 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                       <  SRR4302540.1227573/94‑1 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGCGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                       <  SRR4302540.9741524/94‑1 (MQ=60)
                                         AAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAG                                                      >  SRR4302540.4767439/1‑94 (MQ=60)
                                           GCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAC                                                    <  SRR4302540.5643672/94‑1 (MQ=60)
                                           GCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAC                                                    <  SRR4302540.207750/94‑1 (MQ=60)
                                           GCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAC                                                    <  SRR4302540.207751/94‑1 (MQ=60)
                                            CGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACA                                                   >  SRR4302540.9551067/1‑94 (MQ=60)
                                             GAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAG                                                  <  SRR4302540.4378907/94‑1 (MQ=60)
                                              AAGAACGCATTCAGGCCGATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGG                                                 <  SRR4302540.4989433/94‑1 (MQ=60)
                                               AGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGT                                                <  SRR4302540.1947637/94‑1 (MQ=60)
                                                GAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTA                                               >  SRR4302540.7077539/1‑94 (MQ=60)
                                                 AACTCATTCAGGCGCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                              <  SRR4302540.7345290/94‑1 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                              <  SRR4302540.9677771/94‑1 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                              >  SRR4302540.425406/1‑94 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                              <  SRR4302540.6164491/94‑1 (MQ=60)
                                                  ACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGT                                             <  SRR4302540.9643938/94‑1 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            >  SRR4302540.2516135/1‑94 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            <  SRR4302540.5925189/94‑1 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            <  SRR4302540.1093011/94‑1 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            <  SRR4302540.8190177/94‑1 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            >  SRR4302540.1953639/1‑94 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            <  SRR4302540.9143071/94‑1 (MQ=60)
                                                    GCATTCAGGCCCATAGCGTGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           <  SRR4302540.7359955/94‑1 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           >  SRR4302540.5360725/1‑94 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           >  SRR4302540.1519647/1‑94 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           >  SRR4302540.3296612/1‑94 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAC                                          >  SRR4302540.799962/1‑94 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAG                                          >  SRR4302540.4385228/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGggaca                                         >  SRR4302540.3076433/1‑89 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302540.4590159/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302540.788512/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         <  SRR4302540.1033193/94‑1 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302540.8335861/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302540.4326204/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302540.1781932/1‑94 (MQ=60)
                                                       TTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGA                                        >  SRR4302540.2609752/1‑94 (MQ=60)
                                                       TTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGA                                        >  SRR4302540.9281194/1‑94 (MQ=60)
                                                       TTCAGGCCCATAGCGGGTGCCAGTGCAACTGGAAGGTGGGAAAAAAGTCCCATCATAAACCTGCCGAATGCAGGGATCAGccaggaggggacga                                        >  SRR4302540.9425662/1‑80 (MQ=39)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     <  SRR4302540.6603596/94‑1 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     <  SRR4302540.322101/94‑1 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     <  SRR4302540.7479430/94‑1 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                    <  SRR4302540.6357305/94‑1 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                    <  SRR4302540.4182113/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302540.4383669/94‑1 (MQ=60)
                                                             CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                  <  SRR4302540.2161854/94‑1 (MQ=60)
                                                             CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                  >  SRR4302540.2673430/1‑94 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 <  SRR4302540.7000533/94‑1 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 >  SRR4302540.146752/1‑94 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 >  SRR4302540.6890677/1‑94 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 >  SRR4302540.638862/1‑94 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTGGGCAAACGGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 <  SRR4302540.210966/94‑1 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                <  SRR4302540.7727604/94‑1 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                >  SRR4302540.5444963/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGgcacgcagccatcgc                               >  SRR4302540.1227356/1‑79 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                               >  SRR4302540.5678232/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                               >  SRR4302540.2398656/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                               >  SRR4302540.3073650/1‑94 (MQ=60)
                                                                 TCGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAAACGTCGCT                              <  SRR4302540.5906303/94‑1 (MQ=60)
                                                                 TAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCT                              >  SRR4302540.5729476/1‑94 (MQ=60)
                                                                 TAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCT                              <  SRR4302540.5906301/94‑1 (MQ=60)
                                                                   GCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGG                            <  SRR4302540.4240459/94‑1 (MQ=60)
                                                                    CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                           >  SRR4302540.3408282/1‑94 (MQ=60)
                                                                    CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGC                           >  SRR4302540.6175338/1‑94 (MQ=60)
                                                                     GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                          >  SRR4302540.6233030/1‑94 (MQ=60)
                                                                     GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                          <  SRR4302540.6985767/94‑1 (MQ=60)
                                                                     GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                          <  SRR4302540.5310633/94‑1 (MQ=60)
                                                                     GGGGGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGTA                          <  SRR4302540.6985771/94‑1 (MQ=60)
                                                                      GGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAT                         >  SRR4302540.8389972/1‑94 (MQ=60)
                                                                       GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                        >  SRR4302540.9665408/1‑94 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                       <  SRR4302540.10059105/94‑1 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                       <  SRR4302540.4333665/94‑1 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                       <  SRR4302540.1914956/94‑1 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                      >  SRR4302540.2563356/1‑94 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                      >  SRR4302540.7962923/1‑94 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAAACGTCGCTGGTATCCA                      >  SRR4302540.2563355/1‑94 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATATTACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302540.9691864/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302540.2558075/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     >  SRR4302540.3705131/1‑94 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302540.8529569/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     >  SRR4302540.8274666/1‑94 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302540.3577259/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302540.1698561/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     >  SRR4302540.7869092/1‑94 (MQ=60)
                                                                           CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAGGAAGACGTCGCTGGTATCCATG                    >  SRR4302540.4972815/1‑94 (MQ=60)
                                                                           CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                    <  SRR4302540.4708077/94‑1 (MQ=60)
                                                                            AGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTCTCCATGC                   >  SRR4302540.6819138/1‑94 (MQ=60)
                                                                            AGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGC                   >  SRR4302540.6819137/1‑94 (MQ=60)
                                                                             GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                  <  SRR4302540.1869218/94‑1 (MQ=60)
                                                                             GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                  <  SRR4302540.6383571/94‑1 (MQ=60)
                                                                              TGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCA                 <  SRR4302540.6407345/94‑1 (MQ=60)
                                                                              TGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCA                 <  SRR4302540.4419188/94‑1 (MQ=60)
                                                                              TGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCA                 >  SRR4302540.6569794/1‑94 (MQ=60)
                                                                               GCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAG                <  SRR4302540.8916800/94‑1 (MQ=60)
                                                                                CAGCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               <  SRR4302540.4619213/94‑1 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               <  SRR4302540.3770817/94‑1 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               <  SRR4302540.1334724/94‑1 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               <  SRR4302540.7261426/94‑1 (MQ=60)
                                                                                 AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA              >  SRR4302540.7999618/1‑94 (MQ=60)
                                                                                 AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA              >  SRR4302540.2191613/1‑94 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302540.10087085/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302540.7500366/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            >  SRR4302540.2127515/1‑94 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            >  SRR4302540.254376/1‑94 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            >  SRR4302540.5251047/1‑94 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGACGACGTCGCTGGTATCCATGCCAGCAACG           >  SRR4302540.4370348/1‑94 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           >  SRR4302540.5805463/1‑94 (MQ=60)
                                                                                       CAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCA        >  SRR4302540.311163/1‑94 (MQ=60)
                                                                                       CAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGGATCCATGCCAGAAACGCCA        >  SRR4302540.6492601/1‑94 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA       <  SRR4302540.10071081/94‑1 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA       >  SRR4302540.847679/1‑94 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302540.10022284/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302540.8272908/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302540.6337806/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302540.9572628/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302540.9146231/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      >  SRR4302540.2384656/1‑94 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAA      >  SRR4302540.8027283/1‑94 (MQ=60)
                                                                                          GTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGA     >  SRR4302540.1573518/1‑94 (MQ=60)
                                                                                          GTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGA     >  SRR4302540.2655832/1‑94 (MQ=60)
                                                                                           TTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAA    >  SRR4302540.5119085/1‑94 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT   <  SRR4302540.1570826/94‑1 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT   <  SRR4302540.9311609/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  NC_000913/3893900‑3894086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑