Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,893,993 A→G A82A (GCT→GCC)  yieG ← putative membrane/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,893,9930AG100.0% 916.5 / NA 280A82A (GCT→GCC) yieGputative membrane/transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (141/139);  total (141/139)

Only 100 of 287 total aligned reads displayed.

GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAG  >  NC_000913/3893903‑3894082
                                                                                          |                                                                                         
gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGc                                                                                         >  1:4802107/1‑93 (MQ=255)
gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGc                                                                                         <  2:4335485/93‑1 (MQ=255)
    tGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAAc                                                                                     <  1:4342827/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                    >  1:1246410/1‑93 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                    <  2:505919/93‑1 (MQ=255)
      ccACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAg                                                                                   >  1:3072728/1‑93 (MQ=255)
       cACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGt                                                                                  >  2:1431621/1‑93 (MQ=255)
         cGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTcc                                                                                >  2:1033671/1‑93 (MQ=255)
          ggCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTccc                                                                               >  1:2029353/1‑93 (MQ=255)
            cAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcat                                                                             <  1:4179410/93‑1 (MQ=255)
            cAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcat                                                                             >  1:4149828/1‑93 (MQ=255)
            cAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcat                                                                             <  1:3595520/93‑1 (MQ=255)
              aGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatca                                                                           <  1:2514429/93‑1 (MQ=255)
               gCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatcat                                                                          <  2:4092658/93‑1 (MQ=255)
               gCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatcat                                                                          >  1:2605028/1‑93 (MQ=255)
                 ccATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataa                                                                        >  1:4974311/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                       >  1:268019/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                       >  1:5212508/1‑93 (MQ=255)
                   aTCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCATGTTGGCAAACAGTCCCATCataata                                                                      <  1:3480674/93‑1 (MQ=255)
                    tCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAc                                                                     >  1:4010969/1‑93 (MQ=255)
                    tCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAc                                                                     <  2:256228/93‑1 (MQ=255)
                     cGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACt                                                                    <  1:1511710/93‑1 (MQ=255)
                      gCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTg                                                                   >  1:5788773/1‑93 (MQ=255)
                        cTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGcc                                                                 >  2:4479965/1‑93 (MQ=255)
                        cTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGcc                                                                 >  1:5041236/1‑93 (MQ=255)
                        cTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGcc                                                                 >  1:4652440/1‑93 (MQ=255)
                          gTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGa                                                               <  2:1195576/93‑1 (MQ=255)
                          gTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGa                                                               >  2:5612468/1‑93 (MQ=255)
                          gTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGa                                                               <  2:5365216/93‑1 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                              <  2:332182/93‑1 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                              <  2:846645/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                             <  2:6202732/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                             >  1:3943180/1‑93 (MQ=255)
                             cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATg                                                            >  2:6275813/1‑93 (MQ=255)
                             cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATg                                                            <  1:752066/93‑1 (MQ=255)
                              aaCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGc                                                           >  2:3003143/1‑93 (MQ=255)
                               aCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCa                                                          >  1:3686619/1‑93 (MQ=255)
                                 gACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGc                                                        <  2:3971599/93‑1 (MQ=255)
                                 gACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGc                                                        <  2:758421/93‑1 (MQ=255)
                                   cAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGa                                                      <  1:6212513/93‑1 (MQ=255)
                                    aaaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAt                                                     >  2:3199514/1‑93 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                    <  2:270351/93‑1 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                    <  2:4893826/93‑1 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                    >  2:5305078/1‑93 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                   <  2:2055148/93‑1 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                   >  2:1285629/1‑93 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                   >  2:5831603/1‑93 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                   >  1:1003584/1‑93 (MQ=255)
                                       aGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAg                                                  <  2:3846583/93‑1 (MQ=255)
                                          gaagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACa                                               <  1:1065607/93‑1 (MQ=255)
                                           aagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAg                                              <  1:676414/93‑1 (MQ=255)
                                           aagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAg                                              <  2:683338/93‑1 (MQ=255)
                                           aagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAg                                              <  2:3987928/93‑1 (MQ=255)
                                             gaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGt                                            <  1:2101651/93‑1 (MQ=255)
                                              aaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                           <  1:5243802/93‑1 (MQ=255)
                                               aCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAg                                          <  1:2986814/93‑1 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       <  2:1364167/93‑1 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       >  1:3120251/1‑93 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       <  1:5938445/93‑1 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       >  1:5179960/1‑93 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       <  2:2897444/93‑1 (MQ=255)
                                                   aTTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAc                                      <  1:2745043/93‑1 (MQ=255)
                                                   aTTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAc                                      >  1:3400216/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                     >  1:1148319/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                     >  1:3907016/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                     >  1:6007911/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATACAGCGATCAGACAGGTAGTGACg                                     >  2:3091710/1‑93 (MQ=255)
                                                       aGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAg                                  <  1:148118/93‑1 (MQ=255)
                                                       aGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAg                                  >  1:6264928/1‑93 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 >  1:1625427/1‑93 (MQ=255)
                                                         gCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCTCATCCTAATTTTGCCGAGTGCAGCGATCAGACAGGTGGTGACGAAGAc                                >  1:320208/1‑93 (MQ=255)
                                                           ccATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgt                              <  2:3111321/93‑2 (MQ=255)
                                                            cATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgtc                             <  2:3892169/93‑3 (MQ=255)
                                                             aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                            >  1:5451344/1‑93 (MQ=255)
                                                             aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                            >  1:2447824/1‑93 (MQ=255)
                                                             aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                            >  2:1630292/1‑93 (MQ=255)
                                                             aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                            <  2:5544791/93‑1 (MQ=255)
                                                             aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                            >  1:324586/1‑93 (MQ=255)
                                                             aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                            >  1:4391109/1‑93 (MQ=255)
                                                              tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                           >  2:3699976/1‑93 (MQ=255)
                                                               aGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCt                          <  2:3607796/93‑1 (MQ=255)
                                                                gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                         <  2:2103841/93‑1 (MQ=255)
                                                                gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                         >  1:6128816/1‑93 (MQ=255)
                                                                gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                         >  2:4983273/1‑93 (MQ=255)
                                                                    gTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAt                     >  2:3433591/1‑93 (MQ=255)
                                                                       ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                  >  2:1954045/1‑93 (MQ=255)
                                                                       ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                  >  2:4199372/1‑93 (MQ=255)
                                                                       ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                  >  2:2484701/1‑93 (MQ=255)
                                                                        cAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAt                 >  2:1764384/1‑93 (MQ=255)
                                                                        cAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAt                 >  2:4039736/1‑93 (MQ=255)
                                                                                 tGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAc        >  1:2167730/1‑93 (MQ=255)
                                                                                  ggCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGGATCCACGCCAGCAACg       >  1:841573/1‑93 (MQ=255)
                                                                                   gCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGc      <  1:197042/93‑1 (MQ=255)
                                                                                   gCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGc      >  1:3719146/1‑93 (MQ=255)
                                                                                     aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa    >  2:4781636/1‑93 (MQ=255)
                                                                                     aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa    >  2:5866313/1‑93 (MQ=255)
                                                                                     aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa    >  2:2976581/1‑93 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa   >  2:45589/1‑93 (MQ=255)
                                                                                       gTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAg  <  1:2292888/93‑1 (MQ=255)
                                                                                       gTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAg  >  2:573346/1‑93 (MQ=255)
                                                                                          |                                                                                         
GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAG  >  NC_000913/3893903‑3894082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAAT  >  NC_000913/3893900‑3894085
                                                                                             |                                                                                            
CCCGCCCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                              <  SRR4302533.5513120/94‑1 (MQ=60)
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                              <  SRR4302533.5404478/94‑1 (MQ=60)
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                              <  SRR4302533.3842029/94‑1 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                            <  SRR4302533.2948754/94‑1 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                            >  SRR4302533.4831194/1‑94 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                            <  SRR4302533.2571172/94‑1 (MQ=60)
   GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCA                                                                                           <  SRR4302533.82053/94‑1 (MQ=60)
    ACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAA                                                                                          >  SRR4302533.3110391/1‑94 (MQ=60)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                       >  SRR4302533.1253851/1‑94 (MQ=60)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                       <  SRR4302533.1079021/94‑1 (MQ=60)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                       <  SRR4302533.4368999/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                      <  SRR4302533.4055224/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                      <  SRR4302533.5048069/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                      <  SRR4302533.5212042/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                      >  SRR4302533.3091591/1‑94 (MQ=60)
         CCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGT                                                                                     <  SRR4302533.3922268/94‑1 (MQ=60)
          CACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTC                                                                                    >  SRR4302533.214633/1‑94 (MQ=60)
          CACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTC                                                                                    >  SRR4302533.2352595/1‑94 (MQ=60)
            CGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCC                                                                                  >  SRR4302533.2041878/1‑94 (MQ=60)
              GCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCAT                                                                                >  SRR4302533.4174969/1‑94 (MQ=60)
               CAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATC                                                                               <  SRR4302533.3617641/94‑1 (MQ=60)
               CAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATC                                                                               <  SRR4302533.161698/94‑1 (MQ=60)
               CAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATC                                                                               <  SRR4302533.4204725/94‑1 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                             <  SRR4302533.3070393/94‑1 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                             <  SRR4302533.4850354/94‑1 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                             >  SRR4302533.2621030/1‑94 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                             <  SRR4302533.2529922/94‑1 (MQ=60)
                   CCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAA                                                                           >  SRR4302533.5004472/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGTCAGTGCAACTGGCAGGTTGGGAAACAGTACCATCATAAT                                                                          >  SRR4302533.6129541/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                          >  SRR4302533.269649/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                          <  SRR4302533.5284546/94‑1 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                          >  SRR4302533.3885773/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                          >  SRR4302533.5243898/1‑94 (MQ=60)
                    CCAACGCCAGTACAACGACAAAAGCGGAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                          <  SRR4302533.1989085/94‑1 (MQ=60)
                     CATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATA                                                                         <  SRR4302533.1519926/94‑1 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCATGTTGGCAAACAGTCCCATCATAATAC                                                                        <  SRR4302533.3502146/94‑1 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAC                                                                        >  SRR4302533.4035389/1‑94 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTG                                                                      >  SRR4302533.5823750/1‑94 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTG                                                                      <  SRR4302533.1520821/94‑1 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTG                                                                      >  SRR4302533.4752313/1‑94 (MQ=60)
                         GCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGC                                                                     <  SRR4302533.338967/94‑1 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                    >  SRR4302533.5071747/1‑94 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                    >  SRR4302533.4680633/1‑94 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                   >  SRR4302533.1210134/1‑94 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                   >  SRR4302533.1511982/1‑94 (MQ=60)
                             GTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAA                                                                 >  SRR4302533.3219922/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                >  SRR4302533.3967236/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                >  SRR4302533.394799/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                >  SRR4302533.2898543/1‑94 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAATAGTCCCATCATAATACTGCCGAATG                                                               <  SRR4302533.5571365/94‑1 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                               <  SRR4302533.4407389/94‑1 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                               >  SRR4302533.3427274/1‑94 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                               <  SRR4302533.4897296/94‑1 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAATGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                               >  SRR4302533.3680120/1‑94 (MQ=60)
                                CAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGC                                                              >  SRR4302533.3102096/1‑94 (MQ=60)
                                CAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGC                                                              <  SRR4302533.756497/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                             >  SRR4302533.3709278/1‑94 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                             >  SRR4302533.4486127/1‑94 (MQ=60)
                                   CGACAAAAGCGAAGAACGCATTCAGGCCCATAGTGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGC                                                           <  SRR4302533.5348216/94‑1 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                          <  SRR4302533.3515140/94‑1 (MQ=60)
                                      CAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAT                                                        <  SRR4302533.6249764/94‑1 (MQ=60)
                                      CAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAT                                                        <  SRR4302533.1143731/94‑1 (MQ=60)
                                      CAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAT                                                        >  SRR4302533.1174622/1‑94 (MQ=60)
                                      CAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAT                                                        <  SRR4302533.6210366/94‑1 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                      >  SRR4302533.1009451/1‑94 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                      >  SRR4302533.2788990/1‑94 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                      <  SRR4302533.3174624/94‑1 (MQ=60)
                                         AAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAG                                                     >  SRR4302533.126729/1‑94 (MQ=60)
                                         AAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAG                                                     >  SRR4302533.3241503/1‑94 (MQ=60)
                                          AGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGA                                                    >  SRR4302533.634390/1‑94 (MQ=60)
                                            CGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACA                                                  >  SRR4302533.5913888/1‑94 (MQ=60)
                                             GAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAG                                                 <  SRR4302533.1071865/94‑1 (MQ=60)
                                             GAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAG                                                 <  SRR4302533.1890812/94‑1 (MQ=60)
                                             GAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAG                                                 <  SRR4302533.5272659/94‑1 (MQ=60)
                                              AAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGG                                                <  SRR4302533.680411/94‑1 (MQ=60)
                                                GAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTA                                              <  SRR4302533.882640/94‑1 (MQ=60)
                                                GAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTA                                              <  SRR4302533.2114611/94‑1 (MQ=60)
                                                GAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTA                                              <  SRR4302533.2573146/94‑1 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                             <  SRR4302533.4369561/94‑1 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                             >  SRR4302533.3288926/1‑94 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                             <  SRR4302533.5275320/94‑1 (MQ=60)
                                                  ACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGT                                            <  SRR4302533.3005087/94‑1 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                           >  SRR4302533.5085280/1‑94 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                           >  SRR4302533.5085278/1‑94 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                          >  SRR4302533.3139440/1‑94 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                          <  SRR4302533.6118141/94‑1 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                          >  SRR4302533.5211242/1‑94 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAC                                         <  SRR4302533.2166833/94‑1 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAC                                         <  SRR4302533.5974350/94‑1 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAC                                         >  SRR4302533.128453/1‑94 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAC                                         >  SRR4302533.3421211/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                        >  SRR4302533.106545/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                        >  SRR4302533.6044312/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                        >  SRR4302533.1155175/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                        <  SRR4302533.2761870/94‑1 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                        >  SRR4302533.3930880/1‑94 (MQ=60)
                                                        TCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAA                                      >  SRR4302533.6223016/1‑94 (MQ=60)
                                                        TCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAA                                      <  SRR4302533.3986869/94‑1 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                     >  SRR4302533.6302369/1‑94 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                     <  SRR4302533.1669778/94‑1 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                     <  SRR4302533.2239441/94‑1 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                    >  SRR4302533.231153/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                    >  SRR4302533.799976/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                    >  SRR4302533.1635273/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                    <  SRR4302533.149065/94‑1 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                    >  SRR4302533.2082237/1‑94 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCTCATCCTAATTTTGCCGAGTGCAGCGATCAGACAGGTGGTGACGAAGAC                                   >  SRR4302533.322168/1‑94 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                   <  SRR4302533.4695648/94‑1 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                   >  SRR4302533.3431569/1‑94 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                   <  SRR4302533.3700071/94‑1 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                   <  SRR4302533.3728089/94‑1 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                   <  SRR4302533.2106170/94‑1 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                >  SRR4302533.750536/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                               >  SRR4302533.4417617/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                               >  SRR4302533.2462929/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                               >  SRR4302533.5484183/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                               >  SRR4302533.1620968/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                               >  SRR4302533.326567/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                              >  SRR4302533.3086865/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                              >  SRR4302533.5004821/1‑94 (MQ=60)
                                                                  AGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                            >  SRR4302533.6165749/1‑94 (MQ=60)
                                                                    CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                          >  SRR4302533.5994261/1‑94 (MQ=60)
                                                                    CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                          <  SRR4302533.4166446/94‑1 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                      <  SRR4302533.2641169/94‑1 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                      >  SRR4302533.6274325/1‑94 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                      >  SRR4302533.3240903/1‑94 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                      >  SRR4302533.2501488/1‑94 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                     >  SRR4302533.3711272/1‑94 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                     <  SRR4302533.710294/94‑1 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                     <  SRR4302533.5275009/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                    <  SRR4302533.93464/94‑1 (MQ=60)
                                                                           CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                   <  SRR4302533.3971799/94‑1 (MQ=60)
                                                                             GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                 <  SRR4302533.5996646/94‑1 (MQ=60)
                                                                                 AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA             <  SRR4302533.2642398/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATCATGCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC           <  SRR4302533.2700046/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC           >  SRR4302533.2181063/1‑94 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATatacagcaga          <  SRR4302533.420543/94‑11 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG          <  SRR4302533.3958395/94‑1 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG          <  SRR4302533.4481432/94‑1 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG          >  SRR4302533.4200620/1‑94 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGGATCCACGCCAGCAACG          >  SRR4302533.846493/1‑94 (MQ=60)
                                                                                     GGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGC         >  SRR4302533.3741974/1‑94 (MQ=60)
                                                                                      GCAGGTTGGCAAACAGTCGCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCC        <  SRR4302533.5787521/94‑1 (MQ=60)
                                                                                      GCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCC        <  SRR4302533.198285/94‑1 (MQ=60)
                                                                                      GCAAGTTGGCAAACAGTCCCATCATAATCCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCC        <  SRR4302533.4240049/94‑1 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA      >  SRR4302533.2245894/1‑94 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA      <  SRR4302533.4240041/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG     <  SRR4302533.643754/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG     >  SRR4302533.251235/1‑94 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG     >  SRR4302533.2545257/1‑94 (MQ=60)
                                                                                         GGTTGGCAAACAGTCACATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG     >  SRR4302533.5894790/1‑94 (MQ=60)
                                                                                          GTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGA    <  SRR4302533.2307083/94‑1 (MQ=60)
                                                                                           TTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAA   >  SRR4302533.4455623/1‑94 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT  >  SRR4302533.675948/1‑94 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT  <  SRR4302533.5363428/94‑1 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT  >  SRR4302533.5084416/1‑94 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT  >  SRR4302533.484533/1‑94 (MQ=60)
                                                                                             |                                                                                            
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAAT  >  NC_000913/3893900‑3894085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑