Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,905,748 A→G S83P (TCT→CCT)  pstB ← ATP‑binding component of high‑affinity phosphate‑specific transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,905,7480AG99.8% 1788.0 / NA 554S83P (TCT→CCT) pstBATP‑binding component of high‑affinity phosphate‑specific transport system
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (285/268);  total (285/269)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 567 total aligned reads displayed.

AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA  >  NC_000913/3905658‑3905837
                                                                                          |                                                                                         
aaGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGt                                                                                         >  1:5419615/1‑93 (MQ=255)
aaGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGt                                                                                         >  1:340840/1‑93 (MQ=255)
 aGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGtt                                                                                        >  2:1420487/1‑93 (MQ=255)
   cGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTgg                                                                                      >  1:5031346/1‑93 (MQ=255)
    gATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGt                                                                                     >  1:6502884/1‑93 (MQ=255)
     aTGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTc                                                                                    >  1:62009/1‑93 (MQ=255)
      tgttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCa                                                                                   <  1:247254/93‑1 (MQ=255)
        ttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAgg                                                                                 >  1:691987/1‑93 (MQ=255)
          gtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAt                                                                               <  1:5794002/93‑1 (MQ=255)
           tCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtg                                                                              >  2:9731686/1‑93 (MQ=255)
           tCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtg                                                                              >  1:978578/1‑93 (MQ=255)
           tCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtg                                                                              <  2:2273544/93‑1 (MQ=255)
             gTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgtt                                                                            >  1:4133259/1‑93 (MQ=255)
                gATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTc                                                                         >  2:9075738/1‑93 (MQ=255)
                  tGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGc                                                                       >  2:5004408/1‑93 (MQ=255)
                     aCATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCAt                                                                    <  2:5679038/93‑1 (MQ=255)
                      cATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATc                                                                   <  2:8252900/93‑1 (MQ=255)
                        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCaa                                                                 >  2:2760122/1‑93 (MQ=255)
                        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCaa                                                                 >  2:7311174/1‑93 (MQ=255)
                          ggAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAga                                                               >  2:8749750/1‑92 (MQ=255)
                           gAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                              >  2:3262215/1‑93 (MQ=255)
                            aaaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAg                                                             <  1:592393/93‑1 (MQ=255)
                             aaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                            <  1:1390219/93‑1 (MQ=255)
                              aCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGaa                                                           >  2:6321423/1‑93 (MQ=255)
                                ggCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                                         >  2:7911953/1‑93 (MQ=255)
                                ggCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                                         >  2:1105027/1‑93 (MQ=255)
                                  cGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTc                                                       <  1:3110850/93‑1 (MQ=255)
                                  cGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTc                                                       <  1:10012777/93‑1 (MQ=255)
                                  cGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTc                                                       <  1:7596864/93‑1 (MQ=255)
                                   gTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCa                                                      >  1:7958500/1‑93 (MQ=255)
                                   gTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCa                                                      >  1:9130394/1‑93 (MQ=255)
                                     cGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAcc                                                    >  2:2843493/1‑93 (MQ=255)
                                     cGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAcc                                                    >  1:1635198/1‑93 (MQ=255)
                                       gTTTCTGGAACGCCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCtt                                                  <  2:4630782/93‑1 (MQ=255)
                                       gTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCtt                                                  >  1:5672554/1‑93 (MQ=255)
                                        tttCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                                 >  2:6953600/1‑93 (MQ=255)
                                         ttCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTcc                                                <  1:4694914/93‑1 (MQ=255)
                                         ttCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTcc                                                >  2:1339352/1‑93 (MQ=255)
                                          tCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCg                                               >  2:6856454/1‑93 (MQ=255)
                                            tGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCa                                             >  1:2841679/1‑93 (MQ=255)
                                             ggAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                            <  2:7437851/93‑1 (MQ=255)
                                             ggAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                            >  1:4474141/1‑93 (MQ=255)
                                             ggAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                            >  2:4358791/1‑93 (MQ=255)
                                             ggAACACCATGCCCACTTTCGCACGCAGCACTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                            >  1:8219150/1‑93 (MQ=255)
                                                 cacCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctg                                        >  2:4168103/1‑93 (MQ=255)
                                                  acCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgc                                       >  1:4579715/1‑93 (MQ=255)
                                                   ccATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgct                                      <  2:9579236/93‑1 (MQ=255)
                                                   ccATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgct                                      <  2:7848027/93‑1 (MQ=255)
                                                    cATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                     >  2:4810681/1‑93 (MQ=255)
                                                    cATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                     <  2:2140919/93‑1 (MQ=255)
                                                     aTGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTcc                                    <  1:4865830/93‑1 (MQ=255)
                                                      tGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCg                                   >  1:4030505/1‑93 (MQ=255)
                                                      tGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCg                                   >  1:864767/1‑93 (MQ=255)
                                                      tGCCCACTTTCGCACACAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCg                                   >  1:4953432/1‑93 (MQ=255)
                                                       gCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCgg                                  >  2:6519789/1‑93 (MQ=255)
                                                       gCCCACTTGCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCgg                                  <  2:7695832/93‑1 (MQ=255)
                                                         ccACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGt                                <  2:446616/93‑1 (MQ=255)
                                                         ccACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGt                                >  2:9351002/1‑93 (MQ=255)
                                                          cACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                               >  2:4093655/1‑93 (MQ=255)
                                                          cACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGATGCTCCGGGTa                               >  1:7166319/1‑93 (MQ=255)
                                                           aCTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAc                              <  1:8770729/93‑1 (MQ=255)
                                                            cTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                             <  2:3353394/93‑1 (MQ=255)
                                                            cTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                             <  2:7625060/93‑1 (MQ=255)
                                                             tttCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                            <  1:778497/93‑1 (MQ=255)
                                                             tttCGCACGCAGCAGTGCGATATCCTGAGGGTTGGACAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                            <  2:3560413/93‑1 (MQ=255)
                                                             tttCGCACGCAGCAGCGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                            >  1:94619/1‑93 (MQ=255)
                                                               tCGCACGCAGCAGTGCGATATCCTGAGGGTTGGGCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGtt                          <  1:9676093/93‑1 (MQ=255)
                                                                cgcacgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCTCCTTCCGCACGCTGCTCCGGGTACAGTTc                         <  1:2149646/93‑1 (MQ=255)
                                                                cgcacgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTc                         <  2:5568128/93‑1 (MQ=255)
                                                                  cacgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaa                       <  1:9241397/93‑1 (MQ=255)
                                                                   ccgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaaa                      <  1:738897/92‑1 (MQ=255)
                                                                   acgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaaa                      >  2:1924911/1‑93 (MQ=255)
                                                                    cgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAc                     >  1:5320663/1‑93 (MQ=255)
                                                                      cagcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAt                   >  2:5238993/1‑93 (MQ=255)
                                                                       agcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACTCTGCTCCGGGTACAGTTCAAACAtt                  >  2:6194422/1‑93 (MQ=255)
                                                                       agcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAtt                  <  2:6725797/93‑1 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 >  2:2090101/1‑93 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 >  2:5887534/1‑93 (MQ=255)
                                                                         cagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAtttt                <  2:3025354/93‑1 (MQ=255)
                                                                          agTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttg               >  1:2322135/1‑93 (MQ=255)
                                                                           gTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgt              <  2:2674901/93‑1 (MQ=255)
                                                                             gCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAGACATTttgttg            <  1:1920496/93‑1 (MQ=255)
                                                                             gCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgttg            <  2:853796/93‑1 (MQ=255)
                                                                               gATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGaa          >  1:3696657/1‑93 (MQ=255)
                                                                               gATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGaa          <  2:7123779/93‑1 (MQ=255)
                                                                                atatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAg         <  1:3597050/93‑1 (MQ=255)
                                                                                atatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAg         <  1:8573170/93‑1 (MQ=255)
                                                                                 tatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAgg        >  2:159266/1‑93 (MQ=255)
                                                                                 tatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAgg        >  2:85757/1‑93 (MQ=255)
                                                                                  atCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGt       <  1:9361991/93‑1 (MQ=255)
                                                                                  atCCTGAGGGTTGGTCAGGACGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGt       <  2:3880376/93‑1 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      <  1:2634164/93‑1 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      <  2:1745179/93‑1 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      <  2:8920197/93‑1 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      <  1:1532420/93‑1 (MQ=255)
                                                                                    ccTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAc     >  1:4402402/1‑93 (MQ=255)
                                                                                    ccTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAc     <  1:8062228/93‑1 (MQ=255)
                                                                                     cTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCGCCTTCCGCACGCTGCTCCGGGTACAGTTCAAATATTTTGTTGAAGGTACg    >  1:8960312/1‑93 (MQ=255)
                                                                                     cTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACg    >  1:1120949/1‑93 (MQ=255)
                                                                                       gaggGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACgca  <  1:9048381/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA  >  NC_000913/3905658‑3905837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 306 total aligned reads displayed.
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  >  NC_000913/3905655‑3905841
                                                                                             |                                                                                             
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               >  SRR4302540.3043518/1‑94 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               >  SRR4302540.5518189/1‑94 (MQ=60)
 AAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGG                                                                                              >  SRR4302540.3011148/1‑94 (MQ=60)
  AAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGT                                                                                             >  SRR4302540.1226078/1‑94 (MQ=60)
   AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTT                                                                                            <  SRR4302540.921800/94‑1 (MQ=60)
   AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTT                                                                                            <  SRR4302540.1669208/94‑1 (MQ=60)
   AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTT                                                                                            >  SRR4302540.7667918/1‑94 (MQ=60)
    ATCGATGTTGTCGTAGATGGACATCGGAACCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTG                                                                                           <  SRR4302540.8132522/94‑1 (MQ=60)
    AGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTG                                                                                           <  SRR4302540.8734604/94‑1 (MQ=60)
    AGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTG                                                                                           >  SRR4302540.4465283/1‑94 (MQ=60)
     GCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGG                                                                                          >  SRR4302540.5085791/1‑94 (MQ=60)
     GCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGG                                                                                          <  SRR4302540.9842137/94‑1 (MQ=60)
      CGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGT                                                                                         <  SRR4302540.7940579/94‑1 (MQ=60)
      CGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGT                                                                                         <  SRR4302540.379746/94‑1 (MQ=60)
      CGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGT                                                                                         >  SRR4302540.6575169/1‑94 (MQ=60)
      CGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTGTCTGGAACAACATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGT                                                                                         <  SRR4302540.7940577/94‑1 (MQ=60)
      CGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGCTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGT                                                                                         >  SRR4302540.7775429/1‑94 (MQ=60)
       GATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTC                                                                                        >  SRR4302540.62634/1‑94 (MQ=60)
       GATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTC                                                                                        >  SRR4302540.8822155/1‑94 (MQ=60)
       GATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTC                                                                                        >  SRR4302540.1111719/1‑94 (MQ=60)
         TGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAG                                                                                      <  SRR4302540.249647/94‑1 (MQ=60)
         TGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAG                                                                                      <  SRR4302540.249646/94‑1 (MQ=60)
         TGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAG                                                                                      >  SRR4302540.8903979/1‑94 (MQ=60)
         TGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAG                                                                                      <  SRR4302540.1598221/94‑1 (MQ=60)
         TGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAG                                                                                      >  SRR4302540.2010294/1‑94 (MQ=60)
          GTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGG                                                                                     <  SRR4302540.5661171/94‑1 (MQ=60)
          GTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGG                                                                                     <  SRR4302540.4897751/94‑1 (MQ=60)
          GTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGG                                                                                     >  SRR4302540.6185716/1‑94 (MQ=60)
            TGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAT                                                                                   <  SRR4302540.3492811/94‑1 (MQ=60)
             GTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATG                                                                                  <  SRR4302540.5858024/94‑1 (MQ=60)
             GTCGTAGAGGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATG                                                                                  <  SRR4302540.7726997/94‑1 (MQ=60)
               CGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTT                                                                                >  SRR4302540.4284511/1‑94 (MQ=60)
                  AGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             >  SRR4302540.6316640/1‑94 (MQ=60)
                  AGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             >  SRR4302540.2348298/1‑94 (MQ=60)
                  AGATCGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             <  SRR4302540.5047703/94‑1 (MQ=60)
                   GATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCG                                                                            >  SRR4302540.6832772/1‑94 (MQ=60)
                   GATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCG                                                                            <  SRR4302540.9178354/94‑1 (MQ=60)
                    ATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGC                                                                           >  SRR4302540.4120660/1‑94 (MQ=60)
                     TGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCC                                                                          <  SRR4302540.4957573/94‑1 (MQ=60)
                      GGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCA                                                                         >  SRR4302540.8114682/1‑94 (MQ=60)
                       GACATGGGAAACGGCGGCGTGTTCTGGCACACCATGCCCACTGTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCAT                                                                        <  SRR4302540.306047/94‑1 (MQ=60)
                       GACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATTTTGTCGCCAT                                                                        <  SRR4302540.10106184/94‑1 (MQ=60)
                       GACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCAT                                                                        >  SRR4302540.3161837/1‑94 (MQ=60)
                         CATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCA                                                                      >  SRR4302540.5523987/1‑94 (MQ=60)
                         CATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCA                                                                      >  SRR4302540.9696639/1‑94 (MQ=60)
                         CATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCA                                                                      >  SRR4302540.528274/1‑94 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     <  SRR4302540.1942606/94‑1 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     >  SRR4302540.2321628/1‑94 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     <  SRR4302540.9794870/94‑1 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     >  SRR4302540.7041936/1‑94 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     >  SRR4302540.9636766/1‑94 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     <  SRR4302540.8791789/94‑1 (MQ=60)
                           ACGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAG                                                                    <  SRR4302540.3403357/94‑1 (MQ=60)
                            CGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGC                                                                   <  SRR4302540.4596225/94‑1 (MQ=60)
                            CGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGC                                                                   <  SRR4302540.3561706/94‑1 (MQ=60)
                             GGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCA                                                                  >  SRR4302540.7436932/1‑94 (MQ=60)
                             GGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCA                                                                  <  SRR4302540.4194473/94‑1 (MQ=60)
                             GGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCA                                                                  <  SRR4302540.4746298/94‑1 (MQ=60)
                              GAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAG                                                                 <  SRR4302540.7769257/94‑1 (MQ=60)
                              GAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAG                                                                 >  SRR4302540.1928202/1‑94 (MQ=60)
                              GAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAG                                                                 <  SRR4302540.9108504/94‑1 (MQ=60)
                               AAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGA                                                                >  SRR4302540.5976225/1‑94 (MQ=60)
                                AACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAA                                                               <  SRR4302540.1405099/94‑1 (MQ=60)
                                AACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAA                                                               >  SRR4302540.418339/1‑94 (MQ=60)
                                AACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAA                                                               <  SRR4302540.10021758/94‑1 (MQ=60)
                                 ACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAT                                                              >  SRR4302540.3324008/1‑94 (MQ=60)
                                  CGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATT                                                             >  SRR4302540.5772199/1‑94 (MQ=60)
                                   GGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTT                                                            >  SRR4302540.3678791/1‑94 (MQ=60)
                                   GGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTT                                                            <  SRR4302540.5648957/94‑1 (MQ=60)
                                     CGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCA                                                          >  SRR4302540.9240130/1‑94 (MQ=60)
                                     CGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCA                                                          >  SRR4302540.8051261/1‑94 (MQ=60)
                                     CGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCA                                                          <  SRR4302540.3144879/94‑1 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         >  SRR4302540.7312224/1‑94 (MQ=60)
                                       TCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACC                                                        >  SRR4302540.1653087/1‑94 (MQ=60)
                                       TCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACC                                                        >  SRR4302540.7294524/1‑94 (MQ=60)
                                        CGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCT                                                       <  SRR4302540.1202228/94‑1 (MQ=60)
                                        CGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCT                                                       <  SRR4302540.4029261/94‑1 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      >  SRR4302540.1933012/1‑94 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      >  SRR4302540.5735013/1‑94 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      >  SRR4302540.4670724/1‑94 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      >  SRR4302540.606817/1‑94 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      >  SRR4302540.171698/1‑94 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      <  SRR4302540.7570567/94‑1 (MQ=60)
                                           TTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCC                                                    >  SRR4302540.3683094/1‑94 (MQ=60)
                                            TTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCG                                                   >  SRR4302540.1470502/1‑94 (MQ=60)
                                              CTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCA                                                 >  SRR4302540.9398167/1‑94 (MQ=60)
                                              CTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCA                                                 >  SRR4302540.2872430/1‑94 (MQ=60)
                                               TGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCCTCAAGCAGAATTTCACCTTCCGCAC                                                >  SRR4302540.986998/1‑94 (MQ=60)
                                               TGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAC                                                >  SRR4302540.4522673/1‑94 (MQ=60)
                                               TGGAACACCATGCCCACTTTCGCACGCAGCACTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAC                                                >  SRR4302540.8314681/1‑94 (MQ=60)
                                                 GAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGC                                              >  SRR4302540.8524221/1‑94 (MQ=60)
                                                 GAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGC                                              >  SRR4302540.5333286/1‑94 (MQ=60)
                                                     ACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCT                                          <  SRR4302540.3032585/94‑1 (MQ=60)
                                                     ACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCT                                          >  SRR4302540.5911721/1‑94 (MQ=60)
                                                      CCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTC                                         >  SRR4302540.6711428/1‑94 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        >  SRR4302540.8974160/1‑94 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        <  SRR4302540.6543511/94‑1 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        >  SRR4302540.8346737/1‑94 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       >  SRR4302540.6490558/1‑94 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       >  SRR4302540.4073963/1‑94 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       >  SRR4302540.5991129/1‑94 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       >  SRR4302540.873950/1‑94 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       >  SRR4302540.8677779/1‑94 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       <  SRR4302540.4918015/94‑1 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       >  SRR4302540.8598327/1‑94 (MQ=60)
                                                        ATGCCCACTTTCGCACACAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       >  SRR4302540.5006955/1‑94 (MQ=60)
                                                          GCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGG                                     <  SRR4302540.4757552/94‑1 (MQ=60)
                                                          GCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGG                                     >  SRR4302540.3485703/1‑94 (MQ=60)
                                                          GCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGG                                     >  SRR4302540.1443150/1‑94 (MQ=60)
                                                          GCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCGGCTCGGGG                                     >  SRR4302540.4805425/1‑94 (MQ=60)
                                                            CCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCCCCTTCCGCACGCTGCTCCGGGTA                                   <  SRR4302540.2733287/94‑1 (MQ=60)
                                                            CCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTA                                   >  SRR4302540.4421978/1‑94 (MQ=60)
                                                            CCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTA                                   >  SRR4302540.156171/1‑94 (MQ=60)
                                                            CCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTA                                   >  SRR4302540.3719318/1‑94 (MQ=60)
                                                             CACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAC                                  >  SRR4302540.7168187/1‑94 (MQ=60)
                                                             CACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAC                                  >  SRR4302540.8979130/1‑94 (MQ=60)
                                                             CACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAC                                  >  SRR4302540.9450721/1‑94 (MQ=60)
                                                             CACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCNTCCGCACGCTGCNCNGNGNAC                                  >  SRR4302540.785630/1‑94 (MQ=60)
                                                             CACTGTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTGCCGCACGCTGCTCCGGGTAC                                  <  SRR4302540.6580142/94‑1 (MQ=60)
                                                              ACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACA                                 >  SRR4302540.760308/1‑94 (MQ=60)
                                                              ACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACA                                 >  SRR4302540.5466117/1‑94 (MQ=60)
                                                              ACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACA                                 >  SRR4302540.5466116/1‑94 (MQ=60)
                                                               cttcgcgACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGCTGCCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAG                                <  SRR4302540.4632766/87‑1 (MQ=60)
                                                               CTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAG                                >  SRR4302540.4920952/1‑94 (MQ=60)
                                                               CTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAG                                <  SRR4302540.5065623/94‑1 (MQ=60)
                                                               CTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCAGCAAGCAGAATTTCCCCTTCCGCACGCTGCTCCGGGTACAG                                <  SRR4302540.685838/94‑1 (MQ=60)
                                                               CTTTCGCACGCAGCAGCGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAG                                >  SRR4302540.95623/1‑94 (MQ=60)
                                                                TTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGT                               >  SRR4302540.9937649/1‑94 (MQ=60)
                                                                TTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGT                               <  SRR4302540.786796/94‑1 (MQ=60)
                                                                   CGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCTCCTTCCGCACGCTGCTCCGGGTACAGTTCA                            <  SRR4302540.2172761/94‑1 (MQ=60)
                                                                    GCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAA                           >  SRR4302540.5976194/1‑94 (MQ=60)
                                                                    GCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAA                           <  SRR4302540.4091467/94‑1 (MQ=60)
                                                                     CACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAA                          >  SRR4302540.2513220/1‑94 (MQ=60)
                                                                     CACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAA                          <  SRR4302540.9352086/94‑1 (MQ=60)
                                                                      CCGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAC                         <  SRR4302540.746784/94‑1 (MQ=60)
                                                                      ACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAC                         <  SRR4302540.880884/94‑1 (MQ=60)
                                                                      ACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAC                         >  SRR4302540.5378926/1‑94 (MQ=60)
                                                                       CGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACA                        >  SRR4302540.4478717/1‑94 (MQ=60)
                                                                        GCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAT                       >  SRR4302540.9500846/1‑94 (MQ=60)
                                                                         CAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATT                      >  SRR4302540.2462565/1‑94 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     <  SRR4302540.5293352/94‑1 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     >  SRR4302540.8178733/1‑94 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     <  SRR4302540.2418408/94‑1 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     >  SRR4302540.8178735/1‑94 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     <  SRR4302540.7220862/94‑1 (MQ=60)
                                                                           GCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTT                    <  SRR4302540.9193169/94‑1 (MQ=60)
                                                                           GCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTT                    <  SRR4302540.7071701/94‑1 (MQ=60)
                                                                           GCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTT                    <  SRR4302540.4528854/94‑1 (MQ=60)
                                                                            CAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG                   <  SRR4302540.4088316/94‑1 (MQ=60)
                                                                            CAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG                   >  SRR4302540.4744456/1‑94 (MQ=60)
                                                                            CAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG                   >  SRR4302540.8284424/1‑94 (MQ=60)
                                                                            CAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGGCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG                   >  SRR4302540.2830683/1‑94 (MQ=60)
                                                                             AGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGT                  >  SRR4302540.101460/1‑94 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 >  SRR4302540.4562761/1‑94 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 >  SRR4302540.2240643/1‑94 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 >  SRR4302540.9272710/1‑94 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 >  SRR4302540.9570512/1‑94 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 <  SRR4302540.5822742/94‑1 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 >  SRR4302540.8923980/1‑94 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 <  SRR4302540.5822743/94‑1 (MQ=60)
                                                                                GCGATATCCTGAGGGGTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCGCGCTGCTCCGGGTACAGTTCAAACATTTTGTTGA               <  SRR4302540.2675028/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302540.3345809/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302540.9494887/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              >  SRR4302540.3737015/1‑94 (MQ=60)
                                                                                  GATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAG             <  SRR4302540.7716/94‑1 (MQ=60)
                                                                                  GATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAG             <  SRR4302540.2654447/94‑1 (MQ=60)
                                                                                  GATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAG             >  SRR4302540.9127424/1‑94 (MQ=60)
                                                                                   ATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGG            <  SRR4302540.507083/94‑1 (MQ=60)
                                                                                   ATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGG            <  SRR4302540.3636352/94‑1 (MQ=60)
                                                                                   ATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGG            >  SRR4302540.4715878/1‑94 (MQ=60)
                                                                                   ATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGG            >  SRR4302540.1179465/1‑94 (MQ=60)
                                                                                    TATCCTGAGGGTTGGTCGGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGT           >  SRR4302540.3818596/1‑94 (MQ=60)
                                                                                    TATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGT           <  SRR4302540.2179581/94‑1 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          <  SRR4302540.8703463/94‑1 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          <  SRR4302540.9473475/94‑1 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          <  SRR4302540.1502743/94‑1 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          <  SRR4302540.2072550/94‑1 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          >  SRR4302540.9298368/1‑94 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAGTTTGTTGAAGGTA          >  SRR4302540.9081042/1‑94 (MQ=60)
                                                                                      TCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAC         <  SRR4302540.2662684/94‑1 (MQ=60)
                                                                                      TCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAC         <  SRR4302540.1549387/94‑1 (MQ=60)
                                                                                       TCTGAGGGTTGGTCAGGATGGTGTCGCCCGCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302540.5383547/94‑1 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302540.8155806/94‑1 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        >  SRR4302540.9343815/1‑94 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        >  SRR4302540.1132582/1‑94 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302540.7994691/94‑1 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302540.4458057/94‑1 (MQ=60)
                                                                                        CTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGGACAGTTCAAACATTTTGTTGAAGGTACGC       >  SRR4302540.9733260/1‑94 (MQ=60)
                                                                                          GAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAG     <  SRR4302540.9157004/94‑1 (MQ=60)
                                                                                           AGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGC    >  SRR4302540.9742189/1‑94 (MQ=60)
                                                                                           AGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGC    >  SRR4302540.10140665/1‑94 (MQ=60)
                                                                                           AGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGC    >  SRR4302540.4478107/1‑94 (MQ=60)
                                                                                           AGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGC    >  SRR4302540.8502925/1‑94 (MQ=60)
                                                                                            GGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCA   <  SRR4302540.4715312/94‑1 (MQ=60)
                                                                                            GGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCA   >  SRR4302540.207511/1‑94 (MQ=60)
                                                                                            GGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCA   <  SRR4302540.1107018/94‑1 (MQ=60)
                                                                                            GGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCA   >  SRR4302540.2856282/1‑94 (MQ=60)
                                                                                             GGTTGGTCAGGATTTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  <  SRR4302540.1794908/94‑1 (MQ=60)
                                                                                             GGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  <  SRR4302540.6596654/94‑1 (MQ=60)
                                                                                             GGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  >  SRR4302540.988033/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  >  NC_000913/3905655‑3905841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑