Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,905,748 A→G S83P (TCT→CCT)  pstB ← ATP‑binding component of high‑affinity phosphate‑specific transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,905,7480AG100.0% 809.8 / NA 252S83P (TCT→CCT) pstBATP‑binding component of high‑affinity phosphate‑specific transport system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (125/127);  total (125/127)

Only 100 of 257 total aligned reads displayed.

AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA  >  NC_000913/3905658‑3905837
                                                                                          |                                                                                         
aaGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGt                                                                                         >  2:3876484/1‑93 (MQ=255)
  gCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTg                                                                                       >  1:4123183/1‑93 (MQ=255)
   cGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTgg                                                                                      <  2:5355906/93‑1 (MQ=255)
      tgttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCa                                                                                   >  2:5489465/1‑93 (MQ=255)
       gttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAg                                                                                  >  1:6175836/1‑93 (MQ=255)
        ttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAgg                                                                                 <  2:4189353/93‑1 (MQ=255)
        ttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAgg                                                                                 >  1:1240850/1‑93 (MQ=255)
         tgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGa                                                                                <  1:5046739/93‑1 (MQ=255)
         tgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGa                                                                                <  2:185688/93‑1 (MQ=255)
            cGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgt                                                                             <  1:1395743/93‑1 (MQ=255)
            cGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgt                                                                             <  2:4472871/93‑1 (MQ=255)
             gTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGGTATCCTGAGGGTTGGTCAGGAtgtt                                                                            >  1:6056746/1‑93 (MQ=255)
             gTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgtt                                                                            <  2:3281445/93‑1 (MQ=255)
              tAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttg                                                                           <  1:899838/93‑1 (MQ=255)
               aGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttgt                                                                          <  2:2880855/93‑1 (MQ=255)
               aGATGGACAGCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttgt                                                                          <  2:3565660/93‑1 (MQ=255)
                  tGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGc                                                                       >  2:3817720/1‑93 (MQ=255)
                   ggACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGcc                                                                      <  2:2746134/93‑1 (MQ=255)
                   ggACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGcc                                                                      <  1:473093/93‑1 (MQ=255)
                    gACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCa                                                                     >  2:5194833/1‑93 (MQ=255)
                     aCATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCAt                                                                    >  2:3982194/1‑93 (MQ=255)
                        tCGGAAACGGCGTCGGTTTCTGGAACTCCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCaa                                                                 <  1:4975665/93‑1 (MQ=255)
                        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCaa                                                                 <  2:2765835/93‑1 (MQ=255)
                          ggAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGc                                                               <  1:6045021/93‑1 (MQ=255)
                          ggAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGc                                                               <  2:5889207/93‑1 (MQ=255)
                          ggAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGc                                                               <  2:3345401/93‑1 (MQ=255)
                           gAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                              <  2:298957/93‑1 (MQ=255)
                           gAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                              >  1:926257/1‑93 (MQ=255)
                            aaaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAg                                                             >  1:2761867/1‑93 (MQ=255)
                             aaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                            >  1:531476/1‑93 (MQ=255)
                              aCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGaa                                                           <  2:5424911/93‑1 (MQ=255)
                               cGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAt                                                          >  2:151652/1‑93 (MQ=255)
                                ggCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                                         >  1:3800031/1‑93 (MQ=255)
                                 gCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAttt                                                        <  1:2016294/93‑1 (MQ=255)
                                 gCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAttt                                                        >  1:2120760/1‑93 (MQ=255)
                                    tCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAc                                                     >  1:4776008/1‑93 (MQ=255)
                                    tCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAc                                                     >  1:3980702/1‑93 (MQ=255)
                                    tCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAc                                                     >  1:2476073/1‑93 (MQ=255)
                                      ggTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCt                                                   >  1:4429298/1‑93 (MQ=255)
                                      ggTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCt                                                   >  2:1943607/1‑93 (MQ=255)
                                      ggTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCt                                                   >  2:117112/1‑93 (MQ=255)
                                      ggTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCt                                                   >  2:2167462/1‑93 (MQ=255)
                                      ggTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCt                                                   <  1:5217107/93‑1 (MQ=255)
                                       gTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCtt                                                  >  1:5842720/1‑93 (MQ=255)
                                       gTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCtt                                                  <  1:721233/93‑1 (MQ=255)
                                        tttCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                                 >  1:100704/1‑93 (MQ=255)
                                         ttCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTcc                                                >  1:1484117/1‑93 (MQ=255)
                                         ttCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTcc                                                >  2:3444843/1‑93 (MQ=255)
                                          tCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCg                                               >  1:2839900/1‑93 (MQ=255)
                                            tGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCa                                             <  1:3619002/93‑1 (MQ=255)
                                            tGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCa                                             >  1:2000603/1‑93 (MQ=255)
                                            tGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCa                                             >  2:6190661/1‑93 (MQ=255)
                                            tGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCa                                             >  2:657853/1‑93 (MQ=255)
                                               aaCACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgc                                          >  2:4655845/1‑93 (MQ=255)
                                               aaCACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgc                                          <  1:987513/93‑1 (MQ=255)
                                                acacCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgct                                         <  2:4802815/93‑1 (MQ=255)
                                                acacCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgct                                         <  2:1337522/93‑1 (MQ=255)
                                                 cacCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctg                                        >  1:484487/1‑93 (MQ=255)
                                                  acCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgc                                       <  1:1052690/93‑1 (MQ=255)
                                                   ccATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgct                                      >  2:1634187/1‑93 (MQ=255)
                                                     aTGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTcc                                    >  1:321471/1‑93 (MQ=255)
                                                      tGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCg                                   <  2:2122762/93‑1 (MQ=255)
                                                       gCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCATAATTTCACCTTCCGCACGCTGCTCCgg                                  <  2:1592563/93‑1 (MQ=255)
                                                        cccACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCggg                                 <  1:2194037/93‑1 (MQ=255)
                                                          cACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                               >  2:1399244/1‑93 (MQ=255)
                                                          cACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                               >  2:2623319/1‑93 (MQ=255)
                                                          cACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                               >  1:3638447/1‑93 (MQ=255)
                                                            cTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                             >  2:2540755/1‑93 (MQ=255)
                                                            cTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                             >  2:4276564/1‑93 (MQ=255)
                                                            cTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                             <  2:3190990/93‑1 (MQ=255)
                                                                   acgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaaa                      >  2:4409485/1‑93 (MQ=255)
                                                                   acgcaGCAGTGCGATATCCTGAGGGGTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaaa                      >  1:3105393/1‑93 (MQ=255)
                                                                    cgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAc                     <  1:3780739/93‑1 (MQ=255)
                                                                     gcagcaGTGCGATATTCTGAGGGTTTTTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTGCGCACGCTGCTCCGGGTACAGTTCAAACa                    >  2:2487589/1‑93 (MQ=255)
                                                                       agcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAtt                  <  1:1724496/93‑1 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 <  1:4711053/93‑1 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 <  2:5752859/93‑1 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 >  1:5194610/1‑93 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 >  2:2074597/1‑93 (MQ=255)
                                                                         cagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCTGGGTACAGTTCAAACAtttt                <  2:5143014/93‑1 (MQ=255)
                                                                         cagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAtttt                <  2:5170832/93‑1 (MQ=255)
                                                                          agTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttg               >  1:779878/1‑93 (MQ=255)
                                                                           gTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgt              <  1:3460735/93‑1 (MQ=255)
                                                                           gTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgt              <  1:1859169/93‑1 (MQ=255)
                                                                              ctatatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGa           <  1:3028120/91‑1 (MQ=255)
                                                                              cGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGa           <  2:2615924/93‑1 (MQ=255)
                                                                              cGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGa           >  1:262687/1‑93 (MQ=255)
                                                                               gATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGaa          <  2:5098137/93‑1 (MQ=255)
                                                                                atatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAg         >  1:5318969/1‑93 (MQ=255)
                                                                                 tatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAgg        >  1:2923673/1‑93 (MQ=255)
                                                                                 tatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAgg        <  2:6101581/93‑1 (MQ=255)
                                                                                  atCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGt       <  1:2504248/93‑1 (MQ=255)
                                                                                  atCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGt       >  1:117771/1‑93 (MQ=255)
                                                                                  atCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGt       <  2:3079270/93‑1 (MQ=255)
                                                                                  atCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGt       <  2:2346334/93‑1 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      >  2:3630056/1‑93 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      <  1:1567644/93‑1 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      >  2:4910185/1‑93 (MQ=255)
                                                                                    ccTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAc     <  1:5680727/93‑1 (MQ=255)
                                                                                      tGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACgc   >  1:3327921/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA  >  NC_000913/3905658‑3905837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  >  NC_000913/3905655‑3905841
                                                                                             |                                                                                             
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               <  SRR4302533.1365124/94‑1 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               <  SRR4302533.4362792/94‑1 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               <  SRR4302533.5830106/94‑1 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               >  SRR4302533.1547383/1‑94 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               <  SRR4302533.2153618/94‑1 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               <  SRR4302533.4191024/94‑1 (MQ=60)
 AAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGG                                                                                              >  SRR4302533.5733577/1‑94 (MQ=60)
    AGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTG                                                                                           >  SRR4302533.4148217/1‑94 (MQ=60)
     GCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGG                                                                                          <  SRR4302533.3851829/94‑1 (MQ=60)
      CGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGC                                                                                         >  SRR4302533.2017198/1‑94 (MQ=60)
       GATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTC                                                                                        <  SRR4302533.5274981/94‑1 (MQ=60)
        ATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCA                                                                                       <  SRR4302533.5219813/94‑1 (MQ=60)
         TGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAG                                                                                      <  SRR4302533.874644/94‑1 (MQ=60)
         TGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAG                                                                                      >  SRR4302533.6212942/1‑94 (MQ=60)
          GTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGG                                                                                     >  SRR4302533.1248264/1‑94 (MQ=60)
           TTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATACCCACTGTCGCACGCAGCAGTGCGATATCCTGAGGGTTCGTCAGGT                                                                                    >  SRR4302533.5938726/1‑94 (MQ=60)
            TGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAT                                                                                   <  SRR4302533.5077267/94‑1 (MQ=60)
             GTCGGAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATG                                                                                  >  SRR4302533.3809784/1‑94 (MQ=60)
               CGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGGTATCCTGAGGGTTGGTCAGGATGTT                                                                                >  SRR4302533.6093421/1‑94 (MQ=60)
               CGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTT                                                                                >  SRR4302533.2231101/1‑94 (MQ=60)
               CGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTT                                                                                >  SRR4302533.1678254/1‑94 (MQ=60)
               CGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTT                                                                                <  SRR4302533.1404156/94‑1 (MQ=60)
                 TAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGT                                                                              <  SRR4302533.905118/94‑1 (MQ=60)
                 TAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGT                                                                              >  SRR4302533.3566496/1‑94 (MQ=60)
                  AGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             >  SRR4302533.2845912/1‑94 (MQ=60)
                  AGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             >  SRR4302533.766319/1‑94 (MQ=60)
                  AGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             <  SRR4302533.3066507/94‑1 (MQ=60)
                   GATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCG                                                                            >  SRR4302533.476545/1‑94 (MQ=60)
                   GATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCG                                                                            >  SRR4302533.88727/1‑94 (MQ=60)
                   GATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCG                                                                            <  SRR4302533.3414475/94‑1 (MQ=60)
                    ATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGC                                                                           >  SRR4302533.6081745/1‑94 (MQ=60)
                      GGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCA                                                                         <  SRR4302533.475921/94‑1 (MQ=60)
                        ACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATC                                                                       <  SRR4302533.4358126/94‑1 (MQ=60)
                         CATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCA                                                                      >  SRR4302533.6048409/1‑94 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     >  SRR4302533.4577972/1‑94 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAA                                                                     >  SRR4302533.940215/1‑94 (MQ=60)
                           TCGGAAACGGCGTCGGTTTCTGGAACTCCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAG                                                                    <  SRR4302533.5005831/94‑1 (MQ=60)
                           TCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAG                                                                    <  SRR4302533.2087550/94‑1 (MQ=60)
                           TCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAG                                                                    <  SRR4302533.4839869/94‑1 (MQ=60)
                            CGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGC                                                                   >  SRR4302533.2323466/1‑94 (MQ=60)
                            CGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGC                                                                   >  SRR4302533.3296281/1‑94 (MQ=60)
                             GGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCA                                                                  <  SRR4302533.6081640/94‑1 (MQ=60)
                             GGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCA                                                                  >  SRR4302533.931692/1‑94 (MQ=60)
                              GAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAG                                                                 <  SRR4302533.3423571/94‑1 (MQ=60)
                              GAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAG                                                                 >  SRR4302533.2778800/1‑94 (MQ=60)
                              GAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAG                                                                 >  SRR4302533.5722430/1‑94 (MQ=60)
                               AAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGA                                                                >  SRR4302533.534637/1‑94 (MQ=60)
                                AACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGCTATCCTGAGGGTTGGTCtgactgttgtcgccctccacacgaa                                                               >  SRR4302533.2975873/1‑69 (MQ=60)
                                AACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAA                                                               >  SRR4302533.2201803/1‑94 (MQ=60)
                                  CGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATT                                                             <  SRR4302533.1498792/94‑1 (MQ=60)
                                  CGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATT                                                             >  SRR4302533.3823290/1‑94 (MQ=60)
                                  CGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATT                                                             >  SRR4302533.5438399/1‑94 (MQ=60)
                                   GGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTT                                                            >  SRR4302533.2133835/1‑94 (MQ=60)
                                    GCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTC                                                           <  SRR4302533.546665/94‑1 (MQ=60)
                                    GCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTC                                                           >  SRR4302533.905303/1‑94 (MQ=60)
                                    GCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTC                                                           >  SRR4302533.5036923/1‑94 (MQ=60)
                                    GCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTC                                                           <  SRR4302533.2028716/94‑1 (MQ=60)
                                     CGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCA                                                          >  SRR4302533.3820987/1‑94 (MQ=60)
                                     CGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCA                                                          >  SRR4302533.3062985/1‑94 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         >  SRR4302533.4004938/1‑94 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         >  SRR4302533.4804942/1‑94 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         >  SRR4302533.5347951/1‑94 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         >  SRR4302533.1967366/1‑94 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         >  SRR4302533.2491345/1‑94 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         >  SRR4302533.2915960/1‑94 (MQ=60)
                                       TCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACC                                                        >  SRR4302533.1795197/1‑94 (MQ=60)
                                        CGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCT                                                       >  SRR4302533.4266887/1‑94 (MQ=60)
                                        CGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCT                                                       >  SRR4302533.4456074/1‑94 (MQ=60)
                                        CGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCT                                                       >  SRR4302533.1152758/1‑94 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      >  SRR4302533.5878043/1‑94 (MQ=60)
                                         GGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTT                                                      <  SRR4302533.5248515/94‑1 (MQ=60)
                                          GTTTCTGGCACACCATCCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTC                                                     <  SRR4302533.1311023/94‑1 (MQ=60)
                                          GTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTC                                                     <  SRR4302533.725489/94‑1 (MQ=60)
                                          GTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTC                                                     >  SRR4302533.101331/1‑94 (MQ=60)
                                           TTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCC                                                    <  SRR4302533.665764/94‑1 (MQ=60)
                                           TTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCC                                                    <  SRR4302533.2589032/94‑1 (MQ=60)
                                           TTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCC                                                    >  SRR4302533.1493074/1‑94 (MQ=60)
                                           TTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCC                                                    <  SRR4302533.3646593/94‑1 (MQ=60)
                                           TTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCC                                                    <  SRR4302533.3641488/94‑1 (MQ=60)
                                            TTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCG                                                   >  SRR4302533.2857377/1‑94 (MQ=60)
                                            TTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCG                                                   >  SRR4302533.1430403/1‑94 (MQ=60)
                                            TTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCG                                                   <  SRR4302533.206536/94‑1 (MQ=60)
                                              CTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCA                                                 >  SRR4302533.2012919/1‑94 (MQ=60)
                                              CTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCA                                                 <  SRR4302533.3752573/94‑1 (MQ=60)
                                               TGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAC                                                <  SRR4302533.3641260/94‑1 (MQ=60)
                                               TGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAC                                                >  SRR4302533.3175431/1‑94 (MQ=60)
                                                GGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACG                                               <  SRR4302533.1831102/94‑1 (MQ=60)
                                                  AACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCT                                             <  SRR4302533.2495040/94‑1 (MQ=60)
                                                  AACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCT                                             <  SRR4302533.4759165/94‑1 (MQ=60)
                                                  AACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCT                                             <  SRR4302533.993292/94‑1 (MQ=60)
                                                   ACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTG                                            >  SRR4302533.487383/1‑94 (MQ=60)
                                                     ACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCT                                          <  SRR4302533.1058889/94‑1 (MQ=60)
                                                     ACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCT                                          >  SRR4302533.4963366/1‑94 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        >  SRR4302533.323436/1‑94 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        <  SRR4302533.2767557/94‑1 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        <  SRR4302533.6089772/94‑1 (MQ=60)
                                                        ATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCG                                       <  SRR4302533.1313767/94‑1 (MQ=60)
                                                           CCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGT                                    <  SRR4302533.2207560/94‑1 (MQ=60)
                                                           CCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGT                                    >  SRR4302533.329180/1‑94 (MQ=60)
                                                           CCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGT                                    <  SRR4302533.410271/94‑1 (MQ=60)
                                                            CCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTA                                   >  SRR4302533.3660816/1‑94 (MQ=60)
                                                               CTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAG                                <  SRR4302533.5946188/94‑1 (MQ=60)
                                                               CTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAG                                <  SRR4302533.5236927/94‑1 (MQ=60)
                                                                TTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGT                               <  SRR4302533.2958713/94‑1 (MQ=60)
                                                                   CGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCA                            <  SRR4302533.4744670/94‑1 (MQ=60)
                                                                     CACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAA                          >  SRR4302533.1243555/1‑94 (MQ=60)
                                                                     CACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAA                          <  SRR4302533.5252490/94‑1 (MQ=60)
                                                                     CACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAA                          >  SRR4302533.4837642/1‑94 (MQ=60)
                                                                     CACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAA                          >  SRR4302533.2721522/1‑94 (MQ=60)
                                                                     CACGCAGCAGTGCGATATCCTGAGGGGTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAA                          >  SRR4302533.3124482/1‑94 (MQ=60)
                                                                       CGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACA                        <  SRR4302533.3803899/94‑1 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     >  SRR4302533.5225941/1‑94 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     <  SRR4302533.1734958/94‑1 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     >  SRR4302533.6173010/1‑94 (MQ=60)
                                                                           GCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTT                    <  SRR4302533.5026386/94‑1 (MQ=60)
                                                                           GCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTT                    <  SRR4302533.4739605/94‑1 (MQ=60)
                                                                            CAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG                   >  SRR4302533.784474/1‑94 (MQ=60)
                                                                             AGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGT                  <  SRR4302533.522029/94‑1 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 <  SRR4302533.1870508/94‑1 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 <  SRR4302533.3482097/94‑1 (MQ=60)
                                                                                GCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGA               >  SRR4302533.264278/1‑94 (MQ=60)
                                                                                 CTATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302533.3046651/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              >  SRR4302533.976183/1‑94 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302533.1955464/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302533.2873513/94‑1 (MQ=60)
                                                                                  GATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAG             <  SRR4302533.1028697/94‑1 (MQ=60)
                                                                                  GATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAG             >  SRR4302533.5350846/1‑94 (MQ=60)
                                                                                   ATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGG            >  SRR4302533.2941572/1‑94 (MQ=60)
                                                                                    TATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGT           >  SRR4302533.118520/1‑94 (MQ=60)
                                                                                    TATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGT           <  SRR4302533.1107057/94‑1 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          >  SRR4302533.2545192/1‑94 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          <  SRR4302533.2519688/94‑1 (MQ=60)
                                                                                      TCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAC         <  SRR4302533.1577085/94‑1 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302533.5715038/94‑1 (MQ=60)
                                                                                        CTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGC       >  SRR4302533.3348486/1‑94 (MQ=60)
                                                                                        CTGAGGGTTGGTCAGGATGTTGTCGCCAGCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGC       <  SRR4302533.1567744/94‑1 (MQ=60)
                                                                                         TGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA      >  SRR4302533.6259250/1‑94 (MQ=60)
                                                                                         TGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA      >  SRR4302533.4124676/1‑94 (MQ=60)
                                                                                            GGGTTGATCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCA   <  SRR4302533.5076239/94‑1 (MQ=60)
                                                                                             GGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  <  SRR4302533.6176547/94‑1 (MQ=60)
                                                                                             GGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  <  SRR4302533.2231461/94‑1 (MQ=60)
                                                                                             GGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  <  SRR4302533.5293115/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  >  NC_000913/3905655‑3905841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑