Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,938,723 C→T R28R (CGG→CGA)  yiePs ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,938,7230CT99.5% 2150.4 / ‑5.4 584R28R (CGG→CGA) yiePshypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base T (316/265);  total (316/268)
Fisher's exact test for biased strand distribution p-value = 9.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 598 total aligned reads displayed.

AAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAA  >  NC_000913/3938631‑3938812
                                                                                            |                                                                                         
  gTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCg                                                                                         >  1:5717950/1‑93 (MQ=255)
   tGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGa                                                                                        >  1:4705334/1‑93 (MQ=255)
      tCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTa                                                                                     <  1:664603/93‑1 (MQ=255)
        gATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTAcc                                                                                   >  2:9467083/1‑93 (MQ=255)
        gATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTAcc                                                                                   >  2:7588848/1‑93 (MQ=255)
         aTGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCa                                                                                  >  2:5224868/1‑93 (MQ=255)
           gACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAAt                                                                                <  1:1137961/93‑1 (MQ=255)
              cTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcg                                                                             >  1:931898/1‑93 (MQ=255)
               tgatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgc                                                                            >  1:2849789/1‑93 (MQ=255)
               tgatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgc                                                                            >  1:3747270/1‑93 (MQ=255)
                ggtgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcg                                                                           <  2:8522200/91‑1 (MQ=255)
                gatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcg                                                                           >  1:2686532/1‑93 (MQ=255)
                gatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcg                                                                           >  2:2182785/1‑93 (MQ=255)
                 atgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcgg                                                                          <  1:8779030/93‑1 (MQ=255)
                  tgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggt                                                                         >  1:867484/1‑93 (MQ=255)
                  tgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggt                                                                         >  1:6500978/1‑93 (MQ=255)
                  tgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCCATTGTGGCATGACTCGAGTACCAATtcgcggt                                                                         <  2:8415008/93‑1 (MQ=255)
                   gaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggtc                                                                        >  2:6633062/1‑93 (MQ=255)
                       gTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggtcgcgg                                                                    >  2:3363849/1‑93 (MQ=255)
                         tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTa                                                                  <  1:5331834/93‑1 (MQ=255)
                         tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTa                                                                  >  1:4452631/1‑93 (MQ=255)
                         tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTa                                                                  >  2:411615/1‑93 (MQ=255)
                          ctctTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaa                                                                 >  2:4622941/1‑93 (MQ=255)
                          ctctTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaa                                                                 <  1:3991862/93‑1 (MQ=255)
                          ctctTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaa                                                                 >  1:3002420/1‑93 (MQ=255)
                            cttctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaaaa                                                               >  1:6095040/1‑93 (MQ=255)
                            cttctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaaaa                                                               >  2:9593421/1‑93 (MQ=255)
                             ttctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAc                                                              >  2:3573185/1‑93 (MQ=255)
                              tctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAcc                                                             <  1:8261931/93‑1 (MQ=255)
                                tGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAt                                                           >  1:8005516/1‑93 (MQ=255)
                                 gTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATc                                                          <  2:9383114/93‑1 (MQ=255)
                                   cATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATccc                                                        <  2:8197420/93‑1 (MQ=255)
                                   cATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATccc                                                        >  1:2285911/1‑93 (MQ=255)
                                    aTCCCCCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCt                                                       <  2:1909522/93‑1 (MQ=255)
                                    aTCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCt                                                       <  1:3466581/93‑1 (MQ=255)
                                     tCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCtt                                                      >  2:2309940/1‑93 (MQ=255)
                                     tCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCtt                                                      <  1:4347587/93‑1 (MQ=255)
                                      ccaccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCttt                                                     <  2:8037599/93‑1 (MQ=255)
                                      ccaccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCttt                                                     <  1:7666733/93‑1 (MQ=255)
                                        accaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTg                                                   >  1:4507285/1‑93 (MQ=255)
                                        accaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTg                                                   >  1:10005800/1‑93 (MQ=255)
                                         ccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGc                                                  <  2:7792630/93‑1 (MQ=255)
                                          caGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGcc                                                 <  1:5545900/93‑1 (MQ=255)
                                           aGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCg                                                <  2:6405699/93‑1 (MQ=255)
                                             gTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGtt                                              <  1:1556637/93‑1 (MQ=255)
                                             gTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGtt                                              >  1:6296704/1‑93 (MQ=255)
                                              tAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTa                                             <  1:4613549/93‑1 (MQ=255)
                                              tAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTa                                             >  1:2184294/1‑93 (MQ=255)
                                               aaGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTaa                                            >  2:6729944/1‑93 (MQ=255)
                                                aGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAAc                                           >  2:6142823/1‑93 (MQ=255)
                                                  cAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGt                                         <  1:9770282/93‑1 (MQ=255)
                                                  cAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGt                                         >  2:5492127/1‑93 (MQ=255)
                                                   aaTTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGtt                                        >  1:6949301/1‑93 (MQ=255)
                                                    aTTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGttt                                       <  2:486297/93‑1 (MQ=255)
                                                    aTTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGttt                                       <  1:362294/93‑1 (MQ=255)
                                                     ttCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGTCGTTAACGtttt                                      >  2:7504668/1‑93 (MQ=255)
                                                     ttCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGtttt                                      <  2:8798381/93‑1 (MQ=255)
                                                     ttCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGtttt                                      >  2:6953190/1‑93 (MQ=255)
                                                     ttCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGCTAACGtttt                                      >  1:1869979/1‑93 (MQ=255)
                                                      tCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTg                                     <  2:3010159/93‑1 (MQ=255)
                                                      tCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTg                                     >  2:6536529/1‑93 (MQ=255)
                                                       ccTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGa                                    >  2:1193817/1‑93 (MQ=255)
                                                       ccTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGa                                    <  2:4271760/93‑1 (MQ=255)
                                                          gATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCg                                 <  2:2474167/93‑1 (MQ=255)
                                                          gATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCg                                 >  2:8664388/1‑93 (MQ=255)
                                                           aTCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGc                                <  2:9723735/93‑1 (MQ=255)
                                                            tCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                               >  1:4897723/1‑93 (MQ=255)
                                                            tCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                               <  1:4114342/93‑1 (MQ=255)
                                                             cAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCtt                              <  1:7572438/93‑1 (MQ=255)
                                                             cAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCtt                              >  2:5656040/1‑93 (MQ=255)
                                                              aaGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTc                             <  2:3785213/93‑1 (MQ=255)
                                                                gAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTcgc                           <  1:2007213/93‑1 (MQ=255)
                                                                   aaTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTa                        <  1:5948812/93‑1 (MQ=255)
                                                                    aTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAc                       >  1:9096699/1‑93 (MQ=255)
                                                                    aTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAc                       <  2:5130259/93‑1 (MQ=255)
                                                                    aTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAc                       <  1:1672620/93‑1 (MQ=255)
                                                                     ttCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAcc                      <  1:7123098/93‑1 (MQ=255)
                                                                      tCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCg                     >  2:9017936/1‑93 (MQ=255)
                                                                       ccAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGc                    <  2:1355945/93‑1 (MQ=255)
                                                                       caaGTTCAATTGTGGCATGATTAGTGAACCAATTCTCAGTCGCGGTAAAACCATCCCTTTTGTCGCTAACGCTTTGCCAGATTAGCGTATCgt                    >  2:1604039/3‑92 (MQ=255)
                                                                        cAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCt                   <  1:9529637/93‑1 (MQ=255)
                                                                          gTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTACAACCATCCCTTTTGCCGTTCACGTTTTGACCGCTTCGCGTACCGCTGt                 >  2:7217270/1‑93 (MQ=255)
                                                                          gTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGt                 <  1:2568991/93‑1 (MQ=255)
                                                                           ttCGATTGTGGCATGGCTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTa                <  1:3239293/93‑1 (MQ=255)
                                                                           ttCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTa                <  2:7276896/93‑1 (MQ=255)
                                                                            tCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTAc               <  2:5884315/93‑1 (MQ=255)
                                                                            tCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTAc               <  2:1656718/93‑1 (MQ=255)
                                                                              gATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGa             <  1:7274986/93‑1 (MQ=255)
                                                                                ttGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGact           >  2:2859341/1‑93 (MQ=255)
                                                                                ttGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACCact           >  1:8450410/1‑93 (MQ=255)
                                                                                  gtgGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGactca         <  1:7425126/93‑1 (MQ=255)
                                                                                   tgGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGactcac        >  2:4184857/1‑93 (MQ=255)
                                                                                    ggCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGactccct       >  1:5133099/1‑93 (MQ=255)
                                                                                    ggCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGactcact       >  2:9132553/1‑93 (MQ=255)
                                                                                       aTGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCa    >  2:4756050/1‑93 (MQ=255)
                                                                                        tGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCaa   <  1:7785166/93‑1 (MQ=255)
                                                                                        tGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCaa   >  2:451539/1‑93 (MQ=255)
                                                                                        tGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCaa   >  2:9810457/1‑93 (MQ=255)
                                                                                         gACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCaaa  <  2:2719398/93‑1 (MQ=255)
                                                                                         gACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCaaa  <  2:4002582/93‑1 (MQ=255)
                                                                                            |                                                                                         
AAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAA  >  NC_000913/3938631‑3938812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 312 total aligned reads displayed.
AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGC  >  NC_000913/3938630‑3938816
                                                                                             |                                                                                             
AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACT                                                                                               >  SRR4302540.4729462/1‑94 (MQ=60)
AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACC                                                                                               >  SRR4302540.9234198/1‑94 (MQ=60)
 AAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTC                                                                                              >  SRR4302540.7449363/1‑94 (MQ=60)
 AAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTC                                                                                              >  SRR4302540.6960960/1‑94 (MQ=60)
   GTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGA                                                                                            >  SRR4302540.6584050/1‑94 (MQ=60)
   GTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGA                                                                                            >  SRR4302540.4755613/1‑94 (MQ=60)
    TGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAG                                                                                           <  SRR4302540.2718570/94‑1 (MQ=60)
    TGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAG                                                                                           >  SRR4302540.3656588/1‑94 (MQ=60)
    TGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAG                                                                                           <  SRR4302540.9364900/94‑1 (MQ=60)
     GATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGT                                                                                          >  SRR4302540.8211825/1‑94 (MQ=60)
      ATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGATCGATTGTGGCATGACTCGAGTA                                                                                         >  SRR4302540.3819337/1‑94 (MQ=60)
       TCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTAC                                                                                        <  SRR4302540.671730/94‑1 (MQ=60)
        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                       >  SRR4302540.1617480/1‑94 (MQ=60)
        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                       <  SRR4302540.4734336/94‑1 (MQ=60)
         GATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCA                                                                                      <  SRR4302540.9306130/94‑1 (MQ=60)
          ATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGATCGATTGTGGCATGACTCGAGTACCAA                                                                                     >  SRR4302540.3194528/1‑94 (MQ=60)
           TGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAAT                                                                                    >  SRR4302540.1032040/1‑94 (MQ=60)
           TGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAAT                                                                                    >  SRR4302540.4144415/1‑94 (MQ=60)
            GACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATT                                                                                   >  SRR4302540.2780057/1‑94 (MQ=60)
             ACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTC                                                                                  <  SRR4302540.4802944/94‑1 (MQ=60)
             ACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTC                                                                                  <  SRR4302540.5131082/94‑1 (MQ=60)
             ACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTC                                                                                  <  SRR4302540.4802943/94‑1 (MQ=60)
             ACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTC                                                                                  <  SRR4302540.4439322/94‑1 (MQ=60)
               CTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGC                                                                                >  SRR4302540.6346599/1‑94 (MQ=60)
               CTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGC                                                                                >  SRR4302540.5873315/1‑94 (MQ=60)
                TGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCG                                                                               <  SRR4302540.4463646/94‑1 (MQ=60)
                TGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCG                                                                               >  SRR4302540.7331492/1‑94 (MQ=60)
                TGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCG                                                                               <  SRR4302540.10067373/94‑1 (MQ=60)
                 GATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGG                                                                              >  SRR4302540.6718950/1‑94 (MQ=60)
                  ATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGT                                                                             >  SRR4302540.876687/1‑94 (MQ=60)
                  ATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGT                                                                             <  SRR4302540.8880687/94‑1 (MQ=60)
                  ATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGT                                                                             >  SRR4302540.6573248/1‑94 (MQ=60)
                  ATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGT                                                                             >  SRR4302540.4744506/1‑94 (MQ=60)
                  ATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGC                                                                             >  SRR4302540.7826367/1‑94 (MQ=60)
                   TGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTC                                                                            >  SRR4302540.7344902/1‑94 (MQ=60)
                   TGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTC                                                                            <  SRR4302540.580424/94‑1 (MQ=60)
                    GAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCG                                                                           <  SRR4302540.5590740/94‑1 (MQ=60)
                    GAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCG                                                                           <  SRR4302540.6740237/94‑1 (MQ=60)
                     AAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGC                                                                          >  SRR4302540.8930047/1‑94 (MQ=60)
                      AAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCG                                                                         <  SRR4302540.4053713/94‑1 (MQ=60)
                      AAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCG                                                                         <  SRR4302540.968930/94‑1 (MQ=60)
                       AGTTCTCTTCTGTCATCCACCATTTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGG                                                                        >  SRR4302540.1782147/1‑94 (MQ=60)
                       AGTTCTCTTCTGTCATCCACCAGGTAAGCAATTTCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGG                                                                        >  SRR4302540.7285894/1‑94 (MQ=60)
                       AGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGG                                                                        >  SRR4302540.3548473/1‑94 (MQ=60)
                        GTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGT                                                                       <  SRR4302540.278441/94‑1 (MQ=60)
                        GTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGT                                                                       >  SRR4302540.7535674/1‑94 (MQ=60)
                         TTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTA                                                                      <  SRR4302540.2114245/94‑1 (MQ=60)
                          TCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAA                                                                     <  SRR4302540.5390253/94‑1 (MQ=60)
                          TCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAA                                                                     >  SRR4302540.3035484/1‑94 (MQ=60)
                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                    <  SRR4302540.7666097/94‑1 (MQ=60)
                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                    <  SRR4302540.4034826/94‑1 (MQ=60)
                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                    >  SRR4302540.1847315/1‑94 (MQ=60)
                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                    >  SRR4302540.261435/1‑94 (MQ=60)
                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                    <  SRR4302540.7866347/94‑1 (MQ=60)
                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                    >  SRR4302540.9357741/1‑94 (MQ=60)
                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                    >  SRR4302540.9500464/1‑94 (MQ=60)
                            TCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAA                                                                   >  SRR4302540.7655344/1‑94 (MQ=60)
                            TCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAA                                                                   <  SRR4302540.762888/94‑1 (MQ=60)
                            TCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAA                                                                   >  SRR4302540.6162880/1‑94 (MQ=60)
                            TCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAA                                                                   >  SRR4302540.808289/1‑94 (MQ=60)
                            TCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAA                                                                   >  SRR4302540.8760167/1‑94 (MQ=60)
                             aaccgctcttccgatctagcgcttATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAC                                                                  <  SRR4302540.4679858/70‑1 (MQ=60)
                             CTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAC                                                                  >  SRR4302540.4867726/1‑94 (MQ=60)
                             CTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAC                                                                  >  SRR4302540.8140076/1‑94 (MQ=60)
                              TTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACC                                                                 <  SRR4302540.7075475/94‑1 (MQ=60)
                              TTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACC                                                                 >  SRR4302540.4546878/1‑94 (MQ=60)
                              TTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGCCGCGGTAAAACC                                                                 >  SRR4302540.2592348/1‑94 (MQ=60)
                               TCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCA                                                                >  SRR4302540.1685035/1‑94 (MQ=60)
                               TCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCA                                                                <  SRR4302540.8357936/94‑1 (MQ=60)
                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                               >  SRR4302540.6054127/1‑94 (MQ=60)
                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                               >  SRR4302540.4390752/1‑94 (MQ=60)
                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                               >  SRR4302540.8098675/1‑94 (MQ=60)
                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                               <  SRR4302540.1512210/94‑1 (MQ=60)
                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                               <  SRR4302540.2074676/94‑1 (MQ=60)
                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                               <  SRR4302540.8494731/94‑1 (MQ=60)
                                 TGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATC                                                              <  SRR4302540.2927373/94‑1 (MQ=60)
                                 TGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATC                                                              >  SRR4302540.7947600/1‑94 (MQ=60)
                                 TGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATC                                                              >  SRR4302540.4602366/1‑94 (MQ=60)
                                  GTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCC                                                             >  SRR4302540.3710281/1‑94 (MQ=60)
                                  GGCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCC                                                             <  SRR4302540.8541631/94‑1 (MQ=60)
                                   TCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCC                                                            >  SRR4302540.2310487/1‑94 (MQ=60)
                                     ATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTT                                                          >  SRR4302540.60765/1‑94 (MQ=60)
                                     ATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTT                                                          >  SRR4302540.9441699/1‑94 (MQ=60)
                                     ATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTT                                                          >  SRR4302540.6421795/1‑94 (MQ=60)
                                     ATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTT                                                          <  SRR4302540.3504318/94‑1 (MQ=60)
                                      TCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTT                                                         <  SRR4302540.4394889/94‑1 (MQ=60)
                                      TCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTT                                                         <  SRR4302540.5090054/94‑1 (MQ=60)
                                       CCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTT                                                        <  SRR4302540.3141223/94‑1 (MQ=60)
                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                       >  SRR4302540.5706494/1‑94 (MQ=60)
                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                       >  SRR4302540.9646152/1‑94 (MQ=60)
                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                       >  SRR4302540.508126/1‑94 (MQ=60)
                                         CCCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGC                                                      <  SRR4302540.7716798/94‑1 (MQ=60)
                                          CCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACAATCCCTTTTGCC                                                     >  SRR4302540.8790832/1‑94 (MQ=60)
                                           CAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCG                                                    <  SRR4302540.5606994/94‑1 (MQ=60)
                                            AGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGT                                                   >  SRR4302540.6584579/1‑94 (MQ=60)
                                             GGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTT                                                  >  SRR4302540.6366818/1‑94 (MQ=60)
                                             GGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTT                                                  <  SRR4302540.2712699/94‑1 (MQ=60)
                                             GGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTT                                                  >  SRR4302540.7432703/1‑94 (MQ=60)
                                              GTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTA                                                 <  SRR4302540.1573750/94‑1 (MQ=60)
                                              GTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTA                                                 <  SRR4302540.2016546/94‑1 (MQ=60)
                                              GTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTA                                                 >  SRR4302540.2207870/1‑94 (MQ=60)
                                              GTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTA                                                 >  SRR4302540.3778468/1‑94 (MQ=60)
                                               TAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAA                                                <  SRR4302540.4663051/94‑1 (MQ=60)
                                                AAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGTGGTAAAACCATCCCTTTTGCCGTTAAC                                               <  SRR4302540.141392/94‑1 (MQ=60)
                                                AAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAAG                                               >  SRR4302540.8851390/1‑94 (MQ=60)
                                                 AGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACG                                              <  SRR4302540.868087/94‑1 (MQ=60)
                                                 AGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACG                                              <  SRR4302540.10160255/94‑1 (MQ=60)
                                                 AGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGCGGCATGACTCGAGGACCAATTCGCGGTCGCGGTAAAACCCTCCCTTTTGCCGTTAACG                                              >  SRR4302540.7556825/1‑94 (MQ=60)
                                                  GCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGT                                             >  SRR4302540.4122310/1‑94 (MQ=60)
                                                   CAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTT                                            >  SRR4302540.7026631/1‑94 (MQ=60)
                                                   CAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTT                                            <  SRR4302540.9884838/94‑1 (MQ=60)
                                                   CAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTT                                            >  SRR4302540.2282243/1‑94 (MQ=60)
                                                   CAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTT                                            <  SRR4302540.2724792/94‑1 (MQ=60)
                                                   CAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTT                                            >  SRR4302540.3778480/1‑94 (MQ=60)
                                                    AATTTCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTT                                           <  SRR4302540.7034557/94‑1 (MQ=60)
                                                    AATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTT                                           >  SRR4302540.5335570/1‑94 (MQ=60)
                                                    AATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGGACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTT                                           >  SRR4302540.7960313/1‑94 (MQ=60)
                                                     ATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTT                                          <  SRR4302540.366015/94‑1 (MQ=60)
                                                     ATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTT                                          <  SRR4302540.6995391/94‑1 (MQ=60)
                                                     ATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGAGGTAAAACCATCCCTTCTAGCGTTAACGTTTT                                          >  SRR4302540.7420668/1‑94 (MQ=60)
                                                      TTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTG                                         <  SRR4302540.1753802/94‑1 (MQ=60)
                                                      TTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTG                                         <  SRR4302540.6142432/94‑1 (MQ=60)
                                                       TCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGA                                        <  SRR4302540.3527162/94‑1 (MQ=60)
                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAC                                       >  SRR4302540.5711489/1‑94 (MQ=60)
                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAC                                       <  SRR4302540.3982323/94‑1 (MQ=60)
                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAC                                       >  SRR4302540.4536773/1‑94 (MQ=60)
                                                         CTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACC                                      <  SRR4302540.2955889/94‑1 (MQ=60)
                                                         CTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACC                                      >  SRR4302540.1906563/1‑94 (MQ=60)
                                                          TGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCG                                     >  SRR4302540.199419/1‑94 (MQ=60)
                                                          TGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCG                                     >  SRR4302540.2201318/1‑94 (MQ=60)
                                                           GATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGC                                    <  SRR4302540.2239341/94‑1 (MQ=60)
                                                           GATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGGAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGC                                    >  SRR4302540.1921345/1‑94 (MQ=60)
                                                            ATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCT                                   >  SRR4302540.4950409/1‑94 (MQ=60)
                                                             TCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTT                                  <  SRR4302540.4158764/94‑1 (MQ=60)
                                                               AAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCG                                >  SRR4302540.1636059/1‑94 (MQ=60)
                                                               AAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCG                                <  SRR4302540.4322158/94‑1 (MQ=60)
                                                               AAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCG                                >  SRR4302540.9075057/1‑94 (MQ=60)
                                                               AAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCG                                <  SRR4302540.2886521/94‑1 (MQ=60)
                                                               AAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCCGTAAACCCATCCATTTTGCCGTTAACGTTTTGACCGCTTCG                                >  SRR4302540.878272/1‑94 (MQ=60)
                                                                AGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGC                               >  SRR4302540.7502086/1‑94 (MQ=60)
                                                                 GAANATTNCNGTTCGATNGTGNNNTNNCTCGAGTACCAATTCGCGNTCGCGGTAAAANCATCCNTNTTGCCGTTAACGTTTTGACCGCTTCGCG                              <  SRR4302540.5281432/94‑1 (MQ=60)
                                                                  AAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGT                             >  SRR4302540.3474034/1‑94 (MQ=60)
                                                                   AAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTA                            >  SRR4302540.3340819/1‑94 (MQ=60)
                                                                   AAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTA                            >  SRR4302540.7718047/1‑94 (MQ=60)
                                                                   AAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTA                            >  SRR4302540.3340513/1‑94 (MQ=60)
                                                                    AATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAC                           <  SRR4302540.6014592/94‑1 (MQ=60)
                                                                    AATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAC                           <  SRR4302540.1232957/94‑1 (MQ=60)
                                                                    AATTCCAGTTCGAGTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAC                           <  SRR4302540.1077942/94‑1 (MQ=60)
                                                                     ATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACC                          <  SRR4302540.3839541/94‑1 (MQ=60)
                                                                     ATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACC                          <  SRR4302540.1690799/94‑1 (MQ=60)
                                                                     ATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACC                          <  SRR4302540.5682000/94‑1 (MQ=60)
                                                                      TTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCG                         <  SRR4302540.7202416/94‑1 (MQ=60)
                                                                        CCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCT                       >  SRR4302540.6253365/1‑94 (MQ=60)
                                                                        CCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCT                       <  SRR4302540.8581375/94‑1 (MQ=60)
                                                                        CCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCT                       <  SRR4302540.4651192/94‑1 (MQ=60)
                                                                         CAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTG                      >  SRR4302540.4463363/1‑94 (MQ=60)
                                                                         CAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTG                      <  SRR4302540.9642462/94‑1 (MQ=60)
                                                                          AGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGT                     >  SRR4302540.2364482/1‑94 (MQ=60)
                                                                          AGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGT                     >  SRR4302540.95248/1‑94 (MQ=60)
                                                                          AGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGT                     >  SRR4302540.623647/1‑94 (MQ=60)
                                                                          AGTTCGATTGTGGCAGGACTCGCGTACCAATGCGCGGGCGCGGTACCACCATCCCTTTTGCCGTTAGCGGTTTGACCGCTTCGCGCACCGCTGA                     >  SRR4302540.6366160/1‑94 (MQ=40)
                                                                           GTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTA                    <  SRR4302540.2664711/94‑1 (MQ=60)
                                                                            TTCGATTGTGGCATGGCTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTAC                   <  SRR4302540.3274537/94‑1 (MQ=60)
                                                                            TTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTAC                   <  SRR4302540.2259951/94‑1 (MQ=60)
                                                                             TCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACG                  <  SRR4302540.3234555/94‑1 (MQ=60)
                                                                              CGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGC                 >  SRR4302540.8360139/1‑94 (MQ=60)
                                                                              CGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGA                 >  SRR4302540.5024990/1‑94 (MQ=60)
                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC                <  SRR4302540.4606230/94‑1 (MQ=60)
                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC                <  SRR4302540.7356162/94‑1 (MQ=60)
                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC                >  SRR4302540.5990670/1‑94 (MQ=60)
                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC                <  SRR4302540.2353819/94‑1 (MQ=60)
                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC                >  SRR4302540.7484324/1‑94 (MQ=60)
                                                                                ATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACT               <  SRR4302540.4504890/94‑1 (MQ=60)
                                                                                ATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACT               >  SRR4302540.4515552/1‑94 (MQ=60)
                                                                                ATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACCACT               >  SRR4302540.8548517/1‑94 (MQ=60)
                                                                                  TGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCA             <  SRR4302540.2100850/94‑1 (MQ=60)
                                                                                  TGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCA             >  SRR4302540.4289357/1‑94 (MQ=60)
                                                                                   GTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCAC            <  SRR4302540.6469183/94‑1 (MQ=60)
                                                                                   GTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCAC            <  SRR4302540.5277263/94‑1 (MQ=60)
                                                                                   GTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCAC            <  SRR4302540.7197426/94‑1 (MQ=60)
                                                                                    TGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCCCT           >  SRR4302540.5188690/1‑94 (MQ=60)
                                                                                      GCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCC         >  SRR4302540.5492660/1‑94 (MQ=60)
                                                                                      GCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCC         <  SRR4302540.7658253/94‑1 (MQ=60)
                                                                                      GCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGCTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCC         >  SRR4302540.5492661/1‑94 (MQ=60)
                                                                                       CATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGCACCGCTGTACCACTCACTCCA        >  SRR4302540.39289/1‑94 (MQ=60)
                                                                                        ATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAA       >  SRR4302540.8327797/1‑94 (MQ=60)
                                                                                        ATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAA       >  SRR4302540.8997533/1‑94 (MQ=60)
                                                                                        ATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAA       >  SRR4302540.7754710/1‑94 (MQ=60)
                                                                                        ATGACTCGAGAACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTAGCGTACCGCTGTCAGACTCACTCCAA       >  SRR4302540.2641403/1‑94 (MQ=60)
                                                                                         TGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAA      <  SRR4302540.7876164/94‑1 (MQ=60)
                                                                                           ACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATT    <  SRR4302540.9841357/94‑1 (MQ=60)
                                                                                           ACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATT    <  SRR4302540.10022754/94‑1 (MQ=60)
                                                                                            CTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTG   >  SRR4302540.117580/1‑94 (MQ=60)
                                                                                            CTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTG   >  SRR4302540.7898035/1‑94 (MQ=60)
                                                                                            CTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTG   >  SRR4302540.1062174/1‑94 (MQ=60)
                                                                                            CTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTG   >  SRR4302540.9739642/1‑94 (MQ=60)
                                                                                            CTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTG   >  SRR4302540.9782737/1‑94 (MQ=60)
                                                                                            CTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTCTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTG   >  SRR4302540.6710918/1‑94 (MQ=60)
                                                                                             TCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGC  >  SRR4302540.5207558/1‑94 (MQ=60)
                                                                                             TCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGC  >  SRR4302540.5842492/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGC  >  NC_000913/3938630‑3938816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑